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Engineering an electroactive bacterial cellulose-carbon nanotube composite membrane against Staphylococcus aureus 设计抗金黄色葡萄球菌的电活性细菌纤维素-碳纳米管复合膜
IF 5.9
Biofilm Pub Date : 2025-07-19 DOI: 10.1016/j.bioflm.2025.100305
Daniel S. Levin , Camila S. Cué Royo , Denis Johnson , Soumalya Ghosh , Sricharani Rao Balmuri , Huda Usman , Shakira M. Martínez Vásquez , David Kumar Yesudoss , Abdoulaye Djire , Mostafa Bedewy , Tagbo H.R. Niepa
{"title":"Engineering an electroactive bacterial cellulose-carbon nanotube composite membrane against Staphylococcus aureus","authors":"Daniel S. Levin ,&nbsp;Camila S. Cué Royo ,&nbsp;Denis Johnson ,&nbsp;Soumalya Ghosh ,&nbsp;Sricharani Rao Balmuri ,&nbsp;Huda Usman ,&nbsp;Shakira M. Martínez Vásquez ,&nbsp;David Kumar Yesudoss ,&nbsp;Abdoulaye Djire ,&nbsp;Mostafa Bedewy ,&nbsp;Tagbo H.R. Niepa","doi":"10.1016/j.bioflm.2025.100305","DOIUrl":"10.1016/j.bioflm.2025.100305","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is the leading cause of skin infections in the U.S., and its rapid evolution and resistance to antibiotics create a barrier to effective treatment. In this study, we engineered a composite membrane with bacterial cellulose and carbon nanotubes (BC-CNT) as an electroactive dressing to rapidly eradicate vancomycin-intermediate <em>S. aureus</em>. Nonpathogenic <em>Komagataeibacter sucrofermentans</em> produced the BC membrane at an air-liquid interface. Then, carboxyl-functionalized multi-walled CNTs were integrated into decellularized BC to create stable and electrically conductive BC-CNT dressings. The electric potential and ionic flux across BC-CNT were modeled and standardized via chronoamperometry for experimental validation. We found that treatment with electroactive BC-CNT increases <em>S. aureus</em> sensitivity to vancomycin and prevents macro-scale biofilm formation. The bactericidal efficacy of the composite membrane is consistent with electrochemical stress caused by voltage mediated with BC-CNT. After a single hour of combinatorial electrical and drug treatment, biofilm-forming capacity was inhibited by nearly 92 %. These results advance applications of electrochemistry in medicine and create a new direction to overcome <em>S. aureus</em> infections on skin and soft tissues.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100305"},"PeriodicalIF":5.9,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144694323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus 10058-F4介导对多重耐药金黄色葡萄球菌生物膜形成的抑制作用
IF 5.9
Biofilm Pub Date : 2025-07-16 DOI: 10.1016/j.bioflm.2025.100307
Hiren Dodia , Suvendu Ojha , Puja Chatterjee , Tushar Kant Beuria
{"title":"10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus","authors":"Hiren Dodia ,&nbsp;Suvendu Ojha ,&nbsp;Puja Chatterjee ,&nbsp;Tushar Kant Beuria","doi":"10.1016/j.bioflm.2025.100307","DOIUrl":"10.1016/j.bioflm.2025.100307","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is a global concern that undermines microbial disease treatment and prevention. WHO and World Bank's EcoAMR report predicts that AMR could cause 39 million deaths and $3.4 trillion in annual GDP losses by the year 2050. This is particularly critical with <em>S. aureus</em>, a cause of diverse infections like skin abscesses and pneumonia, where antibiotic resistance increases mortality and hinders treatment. Biofilms are one of the major causes of multi-drug resistance in <em>S. aureus,</em> and their inhibition can restore antibiotic sensitivity. In this study, through screening of the LOPAC drug library, we identified several compounds that exhibit biofilm inhibitory properties against multi-drug-resistant <em>S. aureus</em> without affecting its growth. The compound 10058-F4 was found to have the strongest <em>anti</em>-biofilm activity (&gt;70 % inhibition) with minimal antibacterial effects (MIC 256 μg/mL); however, it showed no inhibitory effects on pre-existing biofilm. Further, the 10058-F4 treatment suppressed the expression of <em>sarA,</em> the biofilm master regulator, along with biofilm genes, such as <em>icaA, fnb, nuc, and sspA</em>. Additionally, the results showed that 10058-F4 synergistically enhanced the antibacterial activity of norfloxacin and tetracycline, indicating its potential use as an adjunct to the existing antibiotic treatments. While these findings suggest the potential of 10058-F4 for clinical use, further investigations are necessary to elucidate its mechanism of action and optimize its application in combination therapies.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100307"},"PeriodicalIF":5.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial structure of yeast biofilms and the role of cell adhesion across different media 酵母生物膜的空间结构及细胞在不同介质上的粘附作用
IF 5.9
Biofilm Pub Date : 2025-07-14 DOI: 10.1016/j.bioflm.2025.100306
Vichi Sicha Irianto , Vítězslav Plocek , Rashim Bharti , Jana Maršíková , Libuše Váchová , Zdena Palková
{"title":"Spatial structure of yeast biofilms and the role of cell adhesion across different media","authors":"Vichi Sicha Irianto ,&nbsp;Vítězslav Plocek ,&nbsp;Rashim Bharti ,&nbsp;Jana Maršíková ,&nbsp;Libuše Váchová ,&nbsp;Zdena Palková","doi":"10.1016/j.bioflm.2025.100306","DOIUrl":"10.1016/j.bioflm.2025.100306","url":null,"abstract":"<div><div>The ability of yeast cells to adhere to solid surfaces or even penetrate semi-solid surfaces and form multicellular biofilms are critical factors in infection. This study examines the relationship between cell adhesion capability and the ability to create spatially organized biofilms in selected <em>Saccharomyces cerevisiae</em> strains, including clinical isolates, and five <em>Candida</em> species (<em>C. albicans</em>, <em>C. glabrata</em>, <em>C. krusei</em>, <em>C. parapsilosis</em>, and <em>C. tropicalis</em>). We assessed cell adhesion to polystyrene surface in four media varying in source of carbon and other nutrients. Using microscopy of vertical cell arrangement profiles within yeast populations grown at the solid-liquid interface, we evaluated their internal organization to determine whether the populations exhibit typical biofilm characteristics, such as the spatial organization of distinct cell types. Results indicate that well adherent <em>S. cerevisiae</em> strains form spatial biofilms with typical internal organization, highlighting strain-specific responses to media composition and supporting the use of natural <em>S. cerevisiae</em> strains for biofilm research. Among <em>Candida</em> species, biofilm formation did not consistently align with adhesion efficiency to plastic; while <em>C. albicans</em> and <em>C. krusei</em> formed spatially structured biofilms on media where they adhered well, <em>C. tropicalis</em> and <em>C. glabrata</em> exhibited efficient adhesion without biofilm structuring. Interestingly, <em>C. parapsilosis</em> formed a structured biofilm despite minimal adhesion. These findings emphasize the role of media composition, particularly components of yeast extract and defined medium for mammalian cell growth RPMI, which differentially impacted adhesion and biofilm formation in <em>S. cerevisiae</em> and <em>C. albicans</em>.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100306"},"PeriodicalIF":5.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial efficacy of on-label vs. hand-mixed irrigation solutions against S. aureus biofilms 标签上与手工混合的灌溉溶液对金黄色葡萄球菌生物膜的抗菌效果
IF 5.9
Biofilm Pub Date : 2025-07-08 DOI: 10.1016/j.bioflm.2025.100304
Robert Falconer , Tyler Smith , David Rothberg , Jeremy Gililland , Nicholas Ashton , Dustin Williams
{"title":"Antimicrobial efficacy of on-label vs. hand-mixed irrigation solutions against S. aureus biofilms","authors":"Robert Falconer ,&nbsp;Tyler Smith ,&nbsp;David Rothberg ,&nbsp;Jeremy Gililland ,&nbsp;Nicholas Ashton ,&nbsp;Dustin Williams","doi":"10.1016/j.bioflm.2025.100304","DOIUrl":"10.1016/j.bioflm.2025.100304","url":null,"abstract":"<div><div>Wound irrigation is routinely performed as part of the DAIR procedure and for hardware infections, yet the most effective irrigation solution for reducing bacterial bioburden is unknown. Clinicians can choose on-label, commercially available irrigation solutions or hand-mix preparations off-label on the operating back table. Current methods for evaluating antiseptic efficacy often do not represent the clinical scenario. Here, we present a proof-of-concept <em>ex vivo</em> setup to evaluate whether on-label, commercially available irrigation solutions were superior at reducing bacterial biofilm burden compared to off-label, hand-mixed irrigation solutions. <em>Staphylococcus aureus</em> ATCC 49525 (Xen36) biofilms were grown on Grade 5 titanium simulated fracture fixation plates and secured to sections of autoclaved bovine femur with cortical bone screws. Fourteen irrigation solutions (three commercial, eleven hand-mixed) and one untreated control group were evaluated by irrigating the biofilm-ridden plates and quantifying the remaining bioburden using a 10-fold dilution series to determine the log<sub>10</sub> reduction. None of the fourteen treatments reduced bioburden statistically significantly compared to the untreated control, where no irrigation was performed. Additionally, no treatment achieved the FDA benchmark of a 4 log<sub>10</sub> reduction for antibacterial activity. An off-label, hand-mixed 0.472 % w/v chlorhexidine gluconate solution reduced the greatest bioburden overall, with a 1.43 ± 0.20 log<sub>10</sub> reduction. On-label irrigation products did not reduce bioburden more than off-label, hand-mixed solutions clinicians often prepare in the operating room. Musculoskeletal infections remain a significant clinical challenge and contribute to increasing healthcare costs. The antimicrobial efficacy of irrigation products should be assessed using clinically relevant models.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100304"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial mechanism of in-situ plasma activated water treatment of pathogenic Escherichia coli and Staphylococcus aureus biofilms 原位等离子体活化水处理致病性大肠杆菌和金黄色葡萄球菌生物膜的抑菌机理
IF 5.9
Biofilm Pub Date : 2025-07-07 DOI: 10.1016/j.bioflm.2025.100303
Binbin Xia , Heema Kumari Nilesh Vyas , Scott A. Rice , Timothy P. Newsome , Patrick J. Cullen , Anne Mai-Prochnow
{"title":"Antimicrobial mechanism of in-situ plasma activated water treatment of pathogenic Escherichia coli and Staphylococcus aureus biofilms","authors":"Binbin Xia ,&nbsp;Heema Kumari Nilesh Vyas ,&nbsp;Scott A. Rice ,&nbsp;Timothy P. Newsome ,&nbsp;Patrick J. Cullen ,&nbsp;Anne Mai-Prochnow","doi":"10.1016/j.bioflm.2025.100303","DOIUrl":"10.1016/j.bioflm.2025.100303","url":null,"abstract":"<div><div>This study investigated the efficacy and mechanisms of inactivation against <em>Escherichia coli</em> UTI89 and <em>Staphylococcus aureus</em> NCTC8325 through an <em>in-situ</em> plasma-activated water (PAW) treatment. PAW was prepared by discharging atmospheric pressure cold plasma beneath the surface of sterile distilled water. The study investigated the inactivation of biofilm cells and biofilm matrix. <em>In situ</em> PAW treatment of both <em>E. coli</em> and <em>S. aureus</em> biofilms resulted in significant reduction in viable cells of 6.76 ± 0.01 log CFU/mL and 6.82 ± 0.02 log CFU/mL, respectively. Notably, relative to <em>E. coli</em>, <em>S. aureus</em> biofilms were rapidly inactivated by 11 min of treatment. Mechanistically, we demonstrate how PAW treatment led to significant biofilm structure disruption, inducing a significant reduction in biofilm biomass and extracellular polymer substances (EPS) matrix. We propose that disruption of the EPS, facilitated greater interaction between PAW and the bacterial cells of the treated biofilms, resulting in significant intracellular reactive oxygen and nitrogen species accumulation as well as significant membrane permeability with disruption to membrane structure resulting in rapid cell death. Collectively, these findings indicate that PAW effectively inactivates biofilms by targeting the biofilm EPS matrix and biofilm cells in both Gram-negative and Gram-positive bacteria. Whilst PAW is an emerging technology, our study underscores PAW as an effective strategy to control bacterial biofilms of both Gram-negative and Gram-positive bacteria, with utility in diverse sectors and industries. Advantageously, we highlight the multiple mechanisms of action of this technology, which possesses a capacity to overcome the challenges of antimicrobial resistance given that it targets multiple components of bacteria and their biofilms.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100303"},"PeriodicalIF":5.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria 相关成像和超分辨率显微镜研究揭示了确定细菌活死状态的复杂性
IF 5.9
Biofilm Pub Date : 2025-07-03 DOI: 10.1016/j.bioflm.2025.100302
Jiaqi Luo , Rasmita Raval
{"title":"Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria","authors":"Jiaqi Luo ,&nbsp;Rasmita Raval","doi":"10.1016/j.bioflm.2025.100302","DOIUrl":"10.1016/j.bioflm.2025.100302","url":null,"abstract":"<div><div>Imaging techniques are widely used to determine the physiological state of bacterial cells and provide an important platform for antibacterial evaluation in biofilms research. The commercial counter-staining SYTO 9 – propidium iodine kit is a popular choice for viability studies, with cell membrane damage due to antimicrobial action leading to replacement of the SYTO 9 dye with propidium iodine. This study investigates the live-dead state of cells in early-stage <em>Staphylococcus aureus</em> biofilms using correlative Fluorescence Microscopy (FM), Scanning Electron Microscopy (SEM) and super resolution Structural Illumination Microscopy (SIM). Correlative imaging data obtained at the single-cell level show that the physiological states of individual bacterial cells indicated by the SYTO 9 – propidium iodine counterstain in FM does not correlate directly with the detailed cell morphology observed by SEM. In addition, SIM was used to map sub-cellular distributions of SYTO 9 – propidium iodine dyes within single cells and revealed greater complexity than hitherto assumed, with 4 different cell-states identified, including double-stained ones and those where SYTO-9 is bound to substances at the cell perimeter. With this knowledge, we present ternary plots to illustrate the significant impacts of this complex staining behaviour on underestimation of cell-membrane damage due to antimicrobial actions and, thus, overestimation of bacterial survival rate in biofilms research.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100302"},"PeriodicalIF":5.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Time-resolved dual transcriptomics of Pseudomonas aeruginosa biofilm formation in cystic fibrosis 囊性纤维化中铜绿假单胞菌生物膜形成的时间分辨双转录组学
IF 5.9
Biofilm Pub Date : 2025-07-02 DOI: 10.1016/j.bioflm.2025.100301
Núria Crua Asensio , Javier Macho Rendón , Juan José González López , Sílvia Gartner Tizzano , Maria Teresa Martín Gómez , Marc Torrent Burgas
{"title":"Time-resolved dual transcriptomics of Pseudomonas aeruginosa biofilm formation in cystic fibrosis","authors":"Núria Crua Asensio ,&nbsp;Javier Macho Rendón ,&nbsp;Juan José González López ,&nbsp;Sílvia Gartner Tizzano ,&nbsp;Maria Teresa Martín Gómez ,&nbsp;Marc Torrent Burgas","doi":"10.1016/j.bioflm.2025.100301","DOIUrl":"10.1016/j.bioflm.2025.100301","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> biofilms cause severe infections in the airways of patients suffering from cystic fibrosis (CF) that are difficult to eradicate, even with intensive antibiotic therapy. The main goal of this study was to define the dual transcriptional response associated with the formation of <em>P. aeruginosa</em> biofilms in a polarized lung epithelium monolayer. We analyzed the dual response of healthy and CF epithelium after infection with <em>P. aeruginosa</em> isolates from acute and chronic infections. Our results show that strains of <em>P. aeruginosa</em> isolated from chronic infections specifically increase the expression of secretion systems of type I, III and VI to hijack the host response. Conversely, strains associated with acute illness use ABC transporters to counteract the antimicrobial response. In return, a distinctive expression pattern in the CF epithelium, including a high degree of cytokine secretion and keratinization, is largely observed in acute infections. Our results show that both host and pathogen genomic backgrounds contribute to the outcome of infection and specific transcriptional signatures could be used in the diagnosis, particularly in CF patients.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100301"},"PeriodicalIF":5.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combating biofilm formation and bacterial killing: N-acetylcysteine's efficacy against Pseudomonas aeruginosa in urinary catheters 对抗生物膜的形成和细菌的杀灭:n -乙酰半胱氨酸对导尿管中铜绿假单胞菌的疗效
IF 5.9
Biofilm Pub Date : 2025-06-25 DOI: 10.1016/j.bioflm.2025.100296
Arthika Manoharan , Greg Whiteley , Rajesh Kuppusamy , Slade Jensen , Trevor Glasbey , Zhuoran Chen , Lia Moshkanbaryans , Kate H. Moore , Theerthankar Das , Jim Manos
{"title":"Combating biofilm formation and bacterial killing: N-acetylcysteine's efficacy against Pseudomonas aeruginosa in urinary catheters","authors":"Arthika Manoharan ,&nbsp;Greg Whiteley ,&nbsp;Rajesh Kuppusamy ,&nbsp;Slade Jensen ,&nbsp;Trevor Glasbey ,&nbsp;Zhuoran Chen ,&nbsp;Lia Moshkanbaryans ,&nbsp;Kate H. Moore ,&nbsp;Theerthankar Das ,&nbsp;Jim Manos","doi":"10.1016/j.bioflm.2025.100296","DOIUrl":"10.1016/j.bioflm.2025.100296","url":null,"abstract":"<div><div>Uropathogenic <em>Pseudomonas aeruginosa</em> is a significant contributor to catheter-associated urinary tract infections (CA-UTIs), distinguished by its unique biofilm-forming properties compared to other strains. Despite its clinical significance, optimized strategies for biofilm eradication in the bladder and on catheters remain limited. Thus, the aim of this study was to highlight the potent antibacterial and biofilm-inhibitory effects of N-acetyl cysteine (NAC) against uropathogenic <em>P. aeruginosa</em>. Additionally, we sought to investigate its effect against catheter obstruction caused by <em>P. aeruginosa</em> in a patient, and whether this phenomenon can be replicated <em>in vitro</em> to underscore the urgency of addressing this critical challenge.</div><div>We demonstrated that uropathogenic <em>P. aeruginosa</em> form thick, mucoid biofilms <em>in vitro</em> that can heavily occlude catheters, with bacterial titres of between 10<sup>8</sup> and 10<sup>11</sup> CFU/cm, thus impairing catheter functionality. Furthermore, treatment with NAC significantly reduced viable bacteria by &gt; 4<sub>log10</sub> (p &lt; 0.01), and inhibited biofilm formation and associated obstruction till experiment endpoint (96h). NAC also displayed significant bactericidal activity (p &lt; 0.001) against <em>P. aeruginosa</em> and significantly impeded bacterial attachment and aggregation through modulation of colloidal forces and change in the structure of the bacterial cell surface, thus impairing the bacterium's ability to initiate biofilm development. Mechanistically, NAC alters the bacterial surface structure, disrupting biofilm-associated virulence.</div><div>Hence our study found that NAC treatment physically disrupts uropathogenic <em>P. aeruginosa</em> biofilms and significantly alters its virulence. Our novel findings highlight the dual bactericidal and anti-biofilm properties of NAC <em>in vitro</em>, offering valuable insights into its potential application for preventing <em>P. aeruginosa</em> biofilm formation and catheter blockage in CA-UTI management.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100296"},"PeriodicalIF":5.9,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vaginal Lactobacillus gasseri biosurfactant: a novel bio- and eco-compatible anti-Candida agent 阴道气体乳杆菌生物表面活性剂:一种新型生物和生态兼容的抗念珠菌剂
IF 5.9
Biofilm Pub Date : 2025-06-24 DOI: 10.1016/j.bioflm.2025.100299
Federica Monti , Barbara Giordani , Stefano Fedi , Daniele Ghezzi , Paola Galletti , Laura Mercolini , Roberto Mandrioli , Carola Parolin , Barbara Luppi , Beatrice Vitali
{"title":"Vaginal Lactobacillus gasseri biosurfactant: a novel bio- and eco-compatible anti-Candida agent","authors":"Federica Monti ,&nbsp;Barbara Giordani ,&nbsp;Stefano Fedi ,&nbsp;Daniele Ghezzi ,&nbsp;Paola Galletti ,&nbsp;Laura Mercolini ,&nbsp;Roberto Mandrioli ,&nbsp;Carola Parolin ,&nbsp;Barbara Luppi ,&nbsp;Beatrice Vitali","doi":"10.1016/j.bioflm.2025.100299","DOIUrl":"10.1016/j.bioflm.2025.100299","url":null,"abstract":"<div><div>Vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC), caused by <em>Candida</em> spp. overgrowth, are common and challenging infections of the genital tract. Among <em>Candida</em> virulence factors, the ability to adhere to host epithelium and to form biofilms are frequently retrieved, especially in RVVC. Vaginal pathogen overgrowth is counteracted by resident lactobacilli, which exert a barrier thanks to the production of antimicrobial metabolites, such as biosurfactants (BS).</div><div>BS was recovered from vaginal <em>Lactobacillus gasseri</em> BC12 and its chemical characteristics as well as its ability to lower the surface tension and to emulsify two different immiscible phases were investigated. BS showed the typical features of a lipopeptide with a critical micellar concentration of 1.2 mg/mL. BS showed antibiofilm activity towards various <em>Candida albicans</em> and non-<em>albicans</em> isolates, notably, it was able to prevent biofilm formation and eradicate preformed biofilms. The absence of cytotoxicity of BS and its ability to counteract the adhesion of <em>Candida</em> spp. were highlighted on HeLa cells through MTT and competition/exclusion assays, respectively. The environmental impact of BS was also investigated on a microcosm model (spring water) by culture-based and molecular (16S rRNA-targeted Illumina sequencing) methods, and no remarkable modifications in the taxonomy composition of the bacterial ecosystem were observed.</div><div>To conclude, BS from <em>L. gasseri</em> BC12 appears as a promising, biocompatible and environmentally friendly approach to prevent and treat VVC/RVVC.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100299"},"PeriodicalIF":5.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of multiple-enzyme treatment on in situ oral biofilm formation in healthy participants 多酶治疗对健康受试者口腔原位生物膜形成的影响
IF 5.9
Biofilm Pub Date : 2025-06-21 DOI: 10.1016/j.bioflm.2025.100298
Pernille Dukanovic Rikvold , Andreas Møllebjerg , Eero Juhani Raittio , Signe Maria Nielsen , Karina Kambourakis Johnsen , Marie Braad Lund , Mette Rose Jørgensen , Rikke Louise Meyer , Sebastian Schlafer
{"title":"The effect of multiple-enzyme treatment on in situ oral biofilm formation in healthy participants","authors":"Pernille Dukanovic Rikvold ,&nbsp;Andreas Møllebjerg ,&nbsp;Eero Juhani Raittio ,&nbsp;Signe Maria Nielsen ,&nbsp;Karina Kambourakis Johnsen ,&nbsp;Marie Braad Lund ,&nbsp;Mette Rose Jørgensen ,&nbsp;Rikke Louise Meyer ,&nbsp;Sebastian Schlafer","doi":"10.1016/j.bioflm.2025.100298","DOIUrl":"10.1016/j.bioflm.2025.100298","url":null,"abstract":"<div><div>Novel approaches for the prevention of biofilm-mediated oral diseases aim to control dental biofilms rather than eradicating bacteria in the mouth. One such approach is the use of enzymes that specifically target and degrade the dental biofilm matrix and thereby facilitate biofilm removal. Matrix-degrading enzymes have consistently shown promising results <em>in vitro</em>, but data on <em>in situ</em>-grown oral biofilms are limited. This study aimed to investigate the effect of combined treatment with mutanase, beta-glucanase and DNase on <em>in situ</em> biofilm formation and removal, microbial biofilm composition and biofilm pH.</div><div>Biofilms from healthy participants were grown for 48 or 72 h on lower-jaw splints and enzyme or control-treated during (3x/day, 30 min) or after growth (30 min). Under the tested conditions, enzyme treatment had no significant effect on biofilm formation or removal compared to control, as assessed by optical coherence tomography and confocal microscopy. Likewise, enzymatic treatment did not induce significant changes in the microbial composition of the biofilms that were dominated by <em>Streptococcus</em>, <em>Haemophilus</em>, <em>Neisseria</em>, <em>Veillonella</em> and <em>Fusobacterium</em> species. The biofilm pH response to a sucrose challenge was assessed using confocal microscopy-based pH ratiometry, and the average biofilm pH was not significantly different between the intervention groups. Under the conditions employed in this study, the tested enzymes had no significant impact on <em>in situ</em> grown biofilms. The treatment regimen, the biofilm composition, or the analytical methods employed may explain the difference to previous results. Further studies are warranted to assess the therapeutic potential of multi-enzyme treatment for dental biofilm control.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"10 ","pages":"Article 100298"},"PeriodicalIF":5.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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