Biofilm最新文献

{"title":"Harnessing emergent properties of microbial consortia for Agriculture: Assembly of the Xilonen SynCom","authors":"Gabriela Gastélum ,&nbsp;Bruno Gómez-Gil ,&nbsp;Gabriela Olmedo-Álvarez ,&nbsp;Jorge Rocha","doi":"10.1016/j.bioflm.2025.100284","DOIUrl":"10.1016/j.bioflm.2025.100284","url":null,"abstract":"<div><div>Synthetic communities (SynComs) are valuable tools for addressing microbial community assembly and function, towards their manipulation for clinical, biotechnological and agricultural applications. However, SynCom design is complicated since interactions between microbes cannot be predicted based on their individual properties. Here we aimed to assemble a functionally cohesive SynCom displaying high-order interactions, as a model to study the community-level beneficial functions of seed-endophytic bacteria from native maize landraces<em>,</em> including strains from the Bacilli class, and the <em>Burkholderia</em> and <em>Pseudomonas</em> genera. We developed a partial combinatorial, bottom-up strategy that was followed by the detection of complex colony architecture as an emergent property in co-cultures. Using this simplified approach, we tested less than 400 co-cultures from a pool of 27 strains, resulting in the assembly the <em>Xilonen</em> SynCom, which includes <em>Bacillus pumilus</em> NME155, <em>Burkholderia contaminans</em> XM7 and <em>Pseudomonas</em> sp. GW6. In this community, higher-order interactions result in complex colony architecture, which is considered a proxy of biofilm formation. Additionally, we generated protocols for absolute quantification of each member from a complex mixture. The <em>Xilonen</em> SynCom will serve as a model to study biofilm formation in community settings, and will aid in the study of the molecular and ecological basis mediating maize fertility.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100284"},"PeriodicalIF":5.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning assisted classification of staphylococcal biofilm maturity","authors":"S.C.J. van Dun ,&nbsp;R. Knol ,&nbsp;A.S. Silva-Herdade ,&nbsp;A.S. Veiga ,&nbsp;M.A.R.B. Castanho ,&nbsp;P.H. Nibbering ,&nbsp;B.G.C.W. Pijls ,&nbsp;A.M. van der Does ,&nbsp;J. Dijkstra ,&nbsp;M.G.J. de Boer","doi":"10.1016/j.bioflm.2025.100283","DOIUrl":"10.1016/j.bioflm.2025.100283","url":null,"abstract":"<div><div>An increasing incidence of device-related, biofilm-associated infections has been observed in clinical practice worldwide. <em>In vitro</em> biofilm models are essential to study these burdensome infections and to design and test potential new treatment approaches. However, there is considerable variation in <em>in vitro</em> biofilm models, and a generally accepted systematic description of biofilm maturity – apart from incubation time – is lacking.</div><div>Therefore, we proposed a scheme comprised of 6 different classes based on common topographic characteristics, <em>i.e.</em>, the substrate, bacterial cells and extracellular matrix, identified by atomic force microscopy (AFM), to describe biofilm maturity independent of incubation time. Evaluation of a test set of staphylococcal biofilm images by a group of independent researchers showed that human observers were capable of classifying images with a mean accuracy of 0.77 ± 0.18. However, manual evaluation of AFM biofilm images is time-consuming, and subject to observer bias. To circumvent these disadvantages, a machine learning algorithm was designed and developed to aid in classification of biofilm images.</div><div>The designed algorithm was capable of identifying pre-set characteristics of biofilms and able to discriminate between the six different classes in the proposed framework. Compared to the established ground truth, the mean accuracy of the developed algorithm amounted to 0.66 ± 0.06 with comparable recall, and off-by-one accuracy of 0.91 ± 0.05. This algorithm, which classifies AFM images of biofilms, has been made available as an open access desktop tool.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100283"},"PeriodicalIF":5.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PMA-qPCR to quantify viable cells in multispecies oral biofilm after disinfectant treatments","authors":"Sybille Schwendener,&nbsp;Manuela Flury,&nbsp;Joël Jenzer,&nbsp;Thomas Thurnheer,&nbsp;Lamprini Karygianni","doi":"10.1016/j.bioflm.2025.100281","DOIUrl":"10.1016/j.bioflm.2025.100281","url":null,"abstract":"<div><div>Conventional quantitative real-time PCR (qPCR) amplifies DNA from viable and dead cells, which can lead to an overestimation of live bacteria. Viability qPCR aims to eliminate DNA from membrane-compromised cells through treatment with propidium monoazide (PMA).</div><div>Here, we evaluated PMA-qPCR to enumerate viable cells of <em>Actinomyces oris</em>, <em>Fusobacterium nucleatum</em>, <em>Streptococcus oralis</em>, <em>Streptococcus mutans,</em> and <em>Veillonella dispar</em>. Five-species oral biofilms were grown on hydroxyapatite discs for 64 h. The biofilms were exposed to 0.2 % chlorhexidine (CHX) or 3 % sodium hypochlorite (NaOCl) for 2 min, either once before cell harvest at 64 h or six times during biofilm growth. The total and single species cells were quantified by culture (CFU) and qPCR from samples with and without PMA treatment before DNA extraction. For species-specific qPCR, TaqMan assays were applied. To determine total bacteria counts, a SYBR green qPCR was established using universal degenerative primers for the conserved <em>dnaK</em> gene.</div><div>For biofilms treated once with CHX, the addition of PMA led to a 1 to 1.6 log₁₀ reduction in PCR counts. This closely matched CFU and PMA-qPCR counts for total bacteria and all single species, except for <em>F. nucleatum</em>, where PMA-qPCR detected significantly more bacteria than culture. NaOCl treatment directly affected DNA and inhibited subsequent PCR amplification, even in samples without PMA. Single treatment of biofilms with 3 % NaOCl and six-fold exposure of biofilms to disinfectants resulted in no viable cell detection by culture. However, PMA did not completely prevent PCR amplification, indicating that disinfectant efficacy measured by viability PCR could be underestimated.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":5.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of nitric oxide donors and EDTA against Pseudomonas aeruginosa biofilms: Implications for antimicrobial therapy in chronic wounds","authors":"Aaron Crowther ,&nbsp;Gareth LuTheryn ,&nbsp;Ramón Garcia-Maset ,&nbsp;Maryam Parhizkar ,&nbsp;J. Mark Sutton ,&nbsp;Charlotte Hind ,&nbsp;Dario Carugo","doi":"10.1016/j.bioflm.2025.100280","DOIUrl":"10.1016/j.bioflm.2025.100280","url":null,"abstract":"<div><div>Opportunistic pathogen <em>Pseudomonas aeruginosa</em> plays a crucial role in chronic wound biofilms, increasing infection's morbidity and mortality. In recent years, the signalling molecule nitric oxide (NO) and chelating agent tetrasodium EDTA (T-EDTA) have been applied therapeutically owing to their multifactorial effects including bacterial killing, biofilm dispersal, and wound healing. However, previous studies assessing NO's antibiofilm efficacy have not considered the variable pH and temperature of the wound environment. Here, pH-dependent NO donors <em>N</em>-diazeniumdiolates (NONOates), PAPA NONOate (PA-NO) and Spermine NONOate (SP–NO), and T-EDTA were applied in wound-relevant pH environments (pH 5.5–8.5) and temperatures (32 °C and 37 °C) to <em>P. aeruginosa</em> PAO1 biofilms grown for either 24 or 48 h. At 32 °C and pH 7.5, 250 μM PA-NO reduced 24-h biofilm biomass by 35 %. At 37 °C, 250 μM PA-NO and 4 % w/v T-EDTA caused 21 % and 57 % biomass reduction in 24-h biofilms, respectively. In 48-h biofilms, NONOates did not induce significant biomass reduction, while T-EDTA maintained its efficacy with a 64 % reduction. A subsequent experiment investigated the impact of NONOates and T-EDTA as pre-treatments before exposure to ciprofloxacin. Unexpectedly, NONOate pre-treatment decreased ciprofloxacin's effectiveness, resulting in approximately 1-log increase in viable planktonic and biofilm-residing cells compared to ciprofloxacin alone. It was hypothesized that this protective effect might stem from NO-induced decreased cellular respiration, which inhibits reactive oxygen species (ROS)-mediated bactericidal mechanisms. These findings highlight both the potential and complexities of developing effective antimicrobial strategies for chronic wound infections, emphasizing the need for further research to optimize treatment approaches.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100280"},"PeriodicalIF":5.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling reciprocal adaptation of Staphylococcus aureus and Pseudomonas aeruginosa co-isolates in artificial sputum medium","authors":"Zhifen Wang ,&nbsp;Emily Giedraitis ,&nbsp;Christiane Knoop ,&nbsp;Daniel J. Breiner ,&nbsp;Vanessa V. Phelan ,&nbsp;Françoise Van Bambeke","doi":"10.1016/j.bioflm.2025.100279","DOIUrl":"10.1016/j.bioflm.2025.100279","url":null,"abstract":"<div><div>Co-infections by <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> are frequent in the airways of patients with cystic fibrosis. These co-infections show higher antibiotic tolerance <em>in vitro</em> compared to mono-infections. <em>In vitro</em> models have been developed to study the interspecies interactions between <em>P. aeruginosa</em> and <em>S. aureus</em>. However, these model systems fail to incorporate clinical isolates with diverse phenotypes, do not reflect the nutritional environment of the CF airway mucus, and/or do not model the biofilm mode of growth observed in the CF airways. Here, we established a dual-species biofilm model grown in artificial sputum medium, where <em>S. aureus</em> was inoculated before <em>P. aeruginosa</em> to facilitate the maintenance of both species over time. It was successfully applied to ten pairs of clinical isolates exhibiting different phenotypes. Co-isolates from individual patients led to robust, stable co-cultures, supporting the theory of cross-adaptation <em>in vivo</em>. Investigation into the cross-adaptation of the VBB496 co-isolate pair revealed that both the <em>P. aeruginosa</em> and <em>S. aureus</em> isolates had reduced antagonism, in part due to reduced production of <em>P. aeruginosa</em> secondary metabolites as well as higher tolerance to those metabolites by <em>S. aureus</em>. Together, these results indicate that the two-species biofilm model system provides a useful tool for exploring interspecies interactions of <em>P. aeruginosa</em> and <em>S. aureus</em> in the context of CF airway infections.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":5.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes","authors":"Nelaveni Rupa ,&nbsp;Pragnya Rao Donthineni ,&nbsp;Sayan Basu ,&nbsp;Kotakonda Arunasri","doi":"10.1016/j.bioflm.2025.100278","DOIUrl":"10.1016/j.bioflm.2025.100278","url":null,"abstract":"<div><div>Vernal keratoconjunctivitis (VKC) is a chronic allergic ocular surface disease with seasonal recurrences and severe forms showing vision threatening complications. The purpose of the study is to understand the prevalence and diversity of biofilm-forming bacteria and antimicrobial resistance in VKC compared to healthy individuals (HC). For this, conjunctival swab samples were collected from VKC (n = 26) and HC (n = 23), of which culture positive samples were 77 % and 78.26 % respectively. The 16S rRNA gene sequencing revealed a significant increase in bacterial diversity in VKC compared to HC (p &lt; 0.05), identifying 16 and 9 bacterial species, respectively. <em>Staphylococcus epidermidis</em> emerged as the predominant bacterium in both groups, with relative abundances of 52.8 % in HC and 30.2 % in VKC (p &lt; 0.001). Biofilm formation was observed in 64.15 % of bacterial species in VKC and 31 % in HC (p &lt; 0.001). Scanning electron microscopy analysis confirmed temporal biofilm formation by <em>Staphylococcus</em> spp. in both groups. Minimum inhibitory concentration testing showed that biofilm forming <em>Staphylococcus</em> spp. from VKC exhibited multidrug resistance (&gt;2 antibiotics) more frequently than those from HC. Additionally, <em>Staphylococcus</em> spp. in VKC demonstrated higher resistance to fluoroquinolones compared to HC. These findings indicate a significantly greater prevalence of biofilm-forming and antimicrobial resistant <em>Staphylococcus</em> bacteria in VKC Patients compared with HC.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100278"},"PeriodicalIF":5.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse reactions of aquaculture biofilter biofilms following acute high-dose peracetic acid","authors":"Wanhe Qi ,&nbsp;Sanni L. Aalto ,&nbsp;Peter Vilhelm Skov ,&nbsp;Kim João de Jesus Gregersen ,&nbsp;Lars-Flemming Pedersen","doi":"10.1016/j.bioflm.2025.100277","DOIUrl":"10.1016/j.bioflm.2025.100277","url":null,"abstract":"<div><div>Peracetic acid (PAA) is an effective disinfectant in aquaculture systems to reduce pathogen loads and improve water quality. However, its effectiveness in disinfecting biofilm in recirculating aquaculture systems (RAS) and resetting biofilters between productions remains unknown. This study evaluated the effects of acute PAA exposure on biofilter biofilms from freshwater RAS. Identical types of bioelements were collected from a pilot-scale RAS (without prior PAA treatment) and a commercial RAS (with PAA treatment), and exposed to PAA concentrations of 0, 1, 2, 4, 8, and 16 mg/L for 1 h. Microbial activity and viability of the exposed biofilms were evaluated using respirometry and flow cytometry. Results showed dose-dependent inhibition of biofilm activity and viability in the pilot-scale RAS. Nitrite oxidation was the most sensitive process to PAA, with an IC₅₀ of 1.27 mg/L (the concentration at which PAA inhibited biofilm metabolic activity by 50 %), followed by ammonia oxidation (IC₅₀ = 1.59 mg/L) and endogenous respiration (IC₅₀ = 2.67 mg/L). Microbial activity linked to H₂O₂ decomposition was least affected (IC₅₀ = 4.68 mg/L). Live cell counts decreased from 9.1 × 10⁷ counts/cm² to 2.4 × 10⁷ counts/cm² of bioelement surface, with dead cells proportion increasing from 15 % to 54 %. In contrast, biofilter biofilms from the commercial RAS exhibited significantly lower sensitivity to PAA dosage, with reductions in nitrite oxidation (39 %) and ammonia oxidation (51 %) observed only at 16 mg/L compared to control. These findings suggest that routine PAA exposure, as part of the other operating conditions on the commercial RAS, can enhance the biofilm's sensitivity to PAA. The study provides new insight into the sensitivity of aquaculture biofilm to PAA treatment and its effect on associated microbial processes.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100277"},"PeriodicalIF":5.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial cellulose: Enhancing productivity and material properties through repeated harvest","authors":"N. Rackov ,&nbsp;N. Janßen ,&nbsp;A. Akkache ,&nbsp;B. Drotleff ,&nbsp;B. Beyer ,&nbsp;E. Scoppola ,&nbsp;N.E. Vrana ,&nbsp;R. Hengge ,&nbsp;C.M. Bidan ,&nbsp;S. Hathroubi","doi":"10.1016/j.bioflm.2025.100276","DOIUrl":"10.1016/j.bioflm.2025.100276","url":null,"abstract":"<div><div>Bacterial cellulose (BC), a promising versatile biopolymer produced by bacteria, has an immense potential in various industries. However, large-scale application is hindered by high production costs and low yields. This study introduces an innovative approach combining a prolonged static culturing with intermittent harvesting. This novel strategy resulted in a significant increase in BC productivity, achieving up to a threefold rise in biomass within the first 35 days. Prolonged growth and continuous harvesting not only enhanced productivity but also led to a mutant strain M2 with higher yields and distinct BC architecture. Mechanical and structural analyses revealed that sequential harvest correlated with increasing crystallinity, altered crystallite sizes, and improved stiffness of the dry material during initial cycles, potentially reflecting bacteria adaptation to resources limitations. Genomic analysis identified key mutations in the M2 strain, including one in the RelA/SpoT enzyme, suggesting a reduced stringent response that promotes growth under nutrient-limiting conditions. Untargeted metabolomic profiling revealed deregulation of several metabolites, including a significant difference in fatty acid metabolites that could potentially influence membrane fluidity and BC secretion. Such metabolic and structural adaptations enhance BC production efficiency and material properties. These findings highlight the potential of intermittent harvesting for sustainable BC production and the role of bacterial adaptation in tuning BC properties. Further research will optimize this strategy and expand its applications in developing tailored biomaterials for diverse industries.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":5.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanowire arrays with programmable geometries as a highly effective anti-biofilm surface","authors":"Marina A. George ,&nbsp;David McGiffin ,&nbsp;Anton Y. Peleg ,&nbsp;Roey Elnathan ,&nbsp;David M. Kaye ,&nbsp;Yue Qu ,&nbsp;Nicolas H. Voelcker","doi":"10.1016/j.bioflm.2025.100275","DOIUrl":"10.1016/j.bioflm.2025.100275","url":null,"abstract":"<div><div>Biofilm-related microbial infections are the Achilles’ heel of many implantable medical devices. Surface patterning with nanostructures in the form of vertically aligned silicon (Si) nanowires (VA-SiNWs) holds promise to prevent these often “incurable” infections. In this study, we fabricated arrays of highly ordered SiNWs varying in three geometric parameters, including height, pitch size, and tip diameter (sharpness). Anti-infective efficacies of fabricated SiNW arrays were assessed against representative laboratory reference bacterial strains, <em>Staphylococcus aureus</em> ATCC 25923 and <em>Escherichia coli</em> ATCC 25922, using a modified microwell biofilm assay representing microorganism-implant interactions at a liquid-solid interface. To further understand the role of individual geometric parameters to the SiNW-induced bacterial killing, SiNW arrays with stepwise changes in individual geometric parameters were compared. The force that NWs applied on bacterial cells was mathematically calculated. Our results suggested that NWs with specific geometries were able to kill adherent bacterial cells and prevent further biofilm formation on biomaterial surfaces. Tip diameter and pitch size appeared to be key factors of nanowires predetermining their anti-infectiveness. Mechanistic investigation found that tip diameter and pitch size co-determined the pressure that NWs put on the cell envelope. The most effective anti-infective NWs fabricated in our study (50 nm in tip diameter and 400 nm in pitch size for <em>S. aureus</em> and 50 nm in tip diameter and 800 nm in pitch size for <em>E. coli</em>) put pressures of approximately 2.79 Pa and 8.86 Pa to the cell envelop of <em>S. aureus</em> and <em>E. coli</em>, respectively, and induced cell lyses. In addition, these NWs retained their activities against clinical isolates of <em>S. aureus</em> and <em>E. coli</em> from patients with confirmed device-related infections and showed little toxicity against human fibroblast cells and red blood cells.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100275"},"PeriodicalIF":5.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the impact of interspecies interactions on biofilm matrix components","authors":"Cristina I. Amador ,&nbsp;Henriette L. Røder ,&nbsp;Jakob Herschend ,&nbsp;Thomas R. Neu ,&nbsp;Mette Burmølle","doi":"10.1016/j.bioflm.2025.100271","DOIUrl":"10.1016/j.bioflm.2025.100271","url":null,"abstract":"<div><div>Multispecies biofilms are complex communities where extracellular polymeric substances (EPS) shape structure, adaptability, and functionality. However, characterizing the components of EPS, particularly glycans and proteins, remains a challenge due to the diverse bacterial species present and their interactions within the matrix. This study examined how interactions between different species affect EPS component composition and spatial organization. We analyzed a consortium of four bacterial soil isolates that have previously demonstrated various intrinsic properties in biofilm communities: <em>Microbacterium oxydans</em>, <em>Paenibacillus amylolyticus</em>, <em>Stenotrophomonas rhizophila</em>, and <em>Xanthomonas retroflexus</em>. We used fluorescence lectin binding analysis to identify specific glycan components and <em>meta</em>-proteomics to characterize matrix proteins in mono- and multispecies biofilms. Our results revealed diverse glycan structures and composition, including fucose and different amino sugar-containing polymers, with substantial differences between monospecies and multispecies biofilms. In isolation, <em>M. oxydans</em> produced galactose/N-Acetylgalactosamine network-like structures and influenced the matrix composition in multispecies biofilms. Proteomic analysis revealed presence of flagellin proteins in <em>X. retroflexus</em> and <em>P. amylolyticus</em>, particularly in multispecies biofilms. Additionally, surface-layer proteins and a unique peroxidase were identified in <em>P. amylolyticus</em> multispecies biofilms, indicating enhanced oxidative stress resistance and structural stability under these conditions. This study highlights the crucial role of interspecies interactions in shaping the biofilm matrix, as well as the production of glycans and proteins, thereby enhancing our understanding of biofilm complexity.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"9 ","pages":"Article 100271"},"PeriodicalIF":5.9,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}