Biofilm最新文献

{"title":"Bacillus subtilis NDmed, a model strain for biofilm genetic studies","authors":"Yasmine Dergham ,&nbsp;Dominique Le Coq ,&nbsp;Arnaud Bridier ,&nbsp;Pilar Sanchez-Vizuete ,&nbsp;Hadi Jbara ,&nbsp;Julien Deschamps ,&nbsp;Kassem Hamze ,&nbsp;Ken-ichi Yoshida ,&nbsp;Marie-Françoise Noirot-Gros ,&nbsp;Romain Briandet","doi":"10.1016/j.bioflm.2023.100152","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100152","url":null,"abstract":"<div><p>The <em>Bacillus subtilis</em> strain NDmed was isolated from an endoscope washer-disinfector in a medical environment. NDmed can form complex macrocolonies with highly wrinkled architectural structures on solid medium. In static liquid culture, it produces thick pellicles at the interface with air as well as remarkable highly protruding ‘‘beanstalk-like’’ submerged biofilm structures at the solid surface. Since these mucoid submerged structures are hyper-resistant to biocides, NDmed has the ability to protect pathogens embedded in mixed-species biofilms by sheltering them from the action of these agents. Additionally, this non-domesticated and highly biofilm forming strain has the propensity of being genetically manipulated. Due to all these properties, the NDmed strain becomes a valuable model for the study of <em>B</em>. <em>subtilis</em> biofilms. This review focuses on several studies performed with NDmed that have highlighted the sophisticated genetic dynamics at play during <em>B</em>. <em>subtilis</em> biofilm formation. Further studies in project using modern molecular tools of advanced technologies with this strain, will allow to deepen our knowledge on the emerging properties of multicellular bacterial life.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100152"},"PeriodicalIF":6.8,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α,α-disubstituted β-amino amides eliminate Staphylococcus aureus biofilms by membrane disruption and biomass removal","authors":"Dominik Ausbacher ,&nbsp;Lindsey A. Miller ,&nbsp;Darla M. Goeres ,&nbsp;Philip S. Stewart ,&nbsp;Morten B. Strøm ,&nbsp;Adyary Fallarero","doi":"10.1016/j.bioflm.2023.100151","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100151","url":null,"abstract":"<div><p>Bacterial biofilms account for up to 80% of all infections and complicate successful therapies due to their intrinsic tolerance to antibiotics. Biofilms also cause serious problems in the industrial sectors, for instance due to the deterioration of metals or microbial contamination of products. Efforts are put in finding novel strategies in both avoiding and fighting biofilms. Biofilm control is achieved by killing and/or removing biofilm or preventing transition to the biofilm lifestyle. Previous research reported on the anti-biofilm potency of α,α-disubstituted β-amino amides <strong>A1</strong>, <strong>A2</strong> and <strong>A3</strong>, which are small antimicrobial peptidomimetics with a molecular weight below 500 Da. In the current study it was investigated if these derivatives cause a fast disintegration of biofilm bacteria and removal of <em>Staphylococcus aureus</em> biofilms. One hour incubation of biofilms with all three derivatives resulted in reduced metabolic activity and membrane permeabilization in <em>S. aureus</em> (ATCC 25923) biofilms. Bactericidal properties of these derivatives were attributed to a direct effect on membranes of biofilm bacteria. The green fluorescence protein expressing <em>Staphylococcus aureus</em> strain AH2547 was cultivated in a CDC biofilm reactor and utilized for disinfectant efficacy testing of <strong>A3</strong>, following the single tube method (<em>American Society for Testing and Materials designation number E2871)</em>. <strong>A3</strong> at a concentration of 90 μM acted as fast as 100 μM chlorhexidine and was equally effective. Confocal laser scanning microscopy studies showed that chlorhexidine treatment lead to fluorescence fading indicating membrane permeabilization but did not cause biomass removal. In contrast, <strong>A3</strong> treatment caused a simultaneous biofilm fluorescence loss and biomass removal. These dual anti-biofilm properties make α,α-disubstituted β-amino amides promising scaffolds in finding new control strategies against recalcitrant biofilms.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100151"},"PeriodicalIF":6.8,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octanoic acid promotes clearance of antibiotic-tolerant cells and eradicates biofilms of Staphylococcus aureus isolated from recurrent bovine mastitis","authors":"Wen-Chun Lin ,&nbsp;Kai-Chen Hsu ,&nbsp;Ming-Feng You ,&nbsp;Kuo-Hua Lee ,&nbsp;Chau-Hwa Chi ,&nbsp;Jyh-Yih Chen","doi":"10.1016/j.bioflm.2023.100149","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100149","url":null,"abstract":"<div><p>Antibiotic therapy is the primary treatment for bovine mastitis, but the drawbacks of this strategy include poor cure rate and economic losses from the need to discard milk with antibiotic residues. Unfortunately, few other treatment options are currently available for mastitis. Failure of antibiotic treatments is often attributed to formation of bacterial biofilms and abscesses in the mammary gland tissue, which lead to chronic infections that are difficult to eradicate and drive recurrent disease. A major mastitis-causing pathogen (MCP) associated with biofilms in bovine mastitis is <em>Staphylococcus aureus</em>. In this study, we demonstrate that octanoic acid has broad-spectrum microbicidal activity against MCPs and effectively inhibits <em>S. aureus</em> biofilm formation in milk (&gt;50% inhibition at 3.13 mM). Octanoic acid effectively clears biofilms (95% eradication at 1<em>X</em> minimum bactericidal concentration, MBC) and infrequently induces <em>S. aureus</em> small colony variants (SCVs) that may cause recurrent mastitis. Additionally, octanoic acid rapidly kills persistent biofilm cells and cells with antibiotic tolerance (within 4 h). In contrast, antibiotics treated at &gt;100<em>X</em> MBC cannot eradicate biofilms but do induce SCVs and antibiotic-tolerant cells. These effects may accelerate the transition from biofilm to chronic infection. Thus, octanoic acid exhibits bactericidal action against <em>S. aureus</em> biofilms, and it is less likely than antibiotic therapy to induce persistent cells and pathogen tolerance. Moreover, octanoic acid acts additively with antibiotics against <em>S. aureus</em>, and it attenuates tetracycline-induced virulence factor gene expression in <em>S. aureus</em> cells. According to these data, octanoic acid may prevent the pathological progression of bovine mastitis and offer a new strategy for treating the condition.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100149"},"PeriodicalIF":6.8,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm","authors":"Ergun Akturk ,&nbsp;Luís D.R. Melo ,&nbsp;Hugo Oliveira ,&nbsp;Aurélie Crabbé ,&nbsp;Tom Coenye ,&nbsp;Joana Azeredo","doi":"10.1016/j.bioflm.2023.100147","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100147","url":null,"abstract":"<div><p>Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em>, which are the prevailing bacterial species that co-infect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested the effect of gentamicin as a co-adjuvant of phages in a dual species-biofilm wound model formed on artificial dermis. The biofilm-killing capacity of the tested treatments was significantly increased when phages were combined with gentamicin and applied multiple times as multiple dose (three doses, every 8 h). Our results suggest that gentamycin is an effective adjuvant of phage therapy particularly when applied simultaneously with phages and in three consecutive doses. The multiple and simultaneous dose treatment seems to be essential to avoid bacterial resistance development to each of the antimicrobial agents.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100147"},"PeriodicalIF":6.8,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cladosporium sphaerospermum extract inhibits quorum sensing associated virulence factors of Serratia marcescens","authors":"Dan-Rui Liu ,&nbsp;Qing-Xiang Yan ,&nbsp;Zheng-Biao Zou ,&nbsp;Chun-Lan Xie ,&nbsp;Xian-Wen Yang ,&nbsp;Ai-Qun Jia","doi":"10.1016/j.bioflm.2023.100146","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100146","url":null,"abstract":"<div><p><em>Serratia marcescens</em> is now becoming a propensity for its highly antimicrobial-resistant clinical infections. Currently, it provides a novel strategy to prevent and control microbial infection by regulating <em>S. marcescens</em> quorum sensing (QS). Deep-sea-derived fungi are rich in QS bioactive constituents. In this work, the extracts from <em>Cladosporium sphaerospermum</em> SCSGAF0054 showed potent QS-related virulence factors and biofilm-inhibiting activities against <em>S. marcescens</em> NJ01<em>.</em> The swimming motility and multiple virulence factors such as prodigiosin, exopolysaccharide (EPS), lipase, protease and hemolysin were moderately inhibited by the extracts at varied concentrations. The confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) images revealed that <em>C. sphaerospermum</em> extracts moderately arrested biofilm formation and cell viability. Further, real-time quantitative PCR (RT-qPCR) analysis revealed that expressions of genes associated with virulence factors, <em>flhD</em>, <em>fimA</em>, <em>fimC</em>, <em>bsmA</em>, <em>bsmB</em>, <em>pigA</em>, <em>pigC</em>, and <em>shlA</em>, were significantly down-regulated compared with control. In addition, the extracts combined with imipenem inhibited the QS system of <em>S. marcescens</em> NJ01, disrupted its preformed biofilm, released the intra-biofilm bacteria and killed the bacteria gradually. Therefore, the extracts combined with imipenem can partially restore bacterial drug sensitivity. These results suggest that the extracts from SCSGAF0054 effectively interfere with the QS system to treat <em>S. marcescens</em> infection alone or combining with classical antimicrobial drugs.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100146"},"PeriodicalIF":6.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate","authors":"Elena Yunda,&nbsp;Quynh Nhu Phan Le,&nbsp;Erik Björn,&nbsp;Madeleine Ramstedt","doi":"10.1016/j.bioflm.2023.100144","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100144","url":null,"abstract":"<div><p><em>Geobacter</em> species are common in iron-rich environments and can contribute to formation of methylmercury (MeHg), a neurotoxic compound with high bioaccumulation potential formed as a result of bacterial and archaeal physiological activity. <em>Geobacter sulfurreducens</em> can utilize various electron acceptors for growth including iron hydroxides or fumarate. However, it remains poorly understood how the growth on these compounds affects physiological properties of bacterial cells in biofilms, including the capacity to produce MeHg. The purpose of this study was to determine changes in the biochemical composition of <em>G. sulfurreducens</em> during biofilm cultivation in media containing iron hydroxide or fumarate, and to quantify mercury (Hg) methylation capacity of the formed biofilms. Biofilms were characterized by Fourier-transform infrared spectroscopy in the attenuated total reflection mode (ATR-FTIR), Resonance Raman spectroscopy and confocal laser scanning microscopy. MeHg formation was quantified by mass spectrometry after incubation of biofilms with 100 nM Hg. The results of ATR-FTIR experiments showed that in presence of fumarate, <em>G. sulfurreducens</em> biofilm formation was accompanied by variation in content of the energy-reserve polymer glycogen over time, which could be cancelled by the addition of supplementary nutrients (yeast extract). In contrast, biofilms cultivated on Fe(III) hydroxide did not accumulate glycogen. The ATR-FTIR results further suggested that Fe(III) hydroxide surfaces bind cells via phosphate and carboxylate groups of bacteria that form complexes with iron. Furthermore, biofilms grown on Fe(III) hydroxide had higher fraction of oxidized cytochromes and produced two to three times less biomass compared to conditions with fumarate. Normalized to biofilm volume, the content of MeHg was similar in assays with biofilms grown on Fe(III) hydroxide and on fumarate (with yeast extract and without). These results suggest that <em>G. sulfurreducens</em> biofilms produce MeHg irrespectively from glycogen content and cytochrome redox state in the cells, and warrant further investigation of the mechanisms controlling this process.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100144"},"PeriodicalIF":6.8,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance of aerobic granular sludge microbiomes to periodic loss of biomass","authors":"Raquel Liébana ,&nbsp;Oskar Modin ,&nbsp;Frank Persson ,&nbsp;Malte Hermansson ,&nbsp;Britt-Marie Wilén","doi":"10.1016/j.bioflm.2023.100145","DOIUrl":"10.1016/j.bioflm.2023.100145","url":null,"abstract":"<div><p>Granular sludge is a biofilm process used for wastewater treatment which is currently being implemented worldwide. It is important to understand how disturbances affect the microbial community and performance of reactors. Here, two acetate-fed replicate reactors were inoculated with acclimatized sludge and the reactor performance, and the granular sludge microbial community succession were studied for 149 days. During this time, the microbial community was challenged by periodically removing half of the reactor biomass, subsequently increasing the food-to-microorganism (F/M) ratio. Diversity analysis together with null models show that overall, the microbial communities were resistant to the disturbances, observing some minor effects on polyphosphate-accumulating and denitrifying microbial communities and their associated reactor functions. Community turnover was driven by drift and random granule loss, and stochasticity was the governing ecological process for community assembly. These results evidence the aerobic granular sludge process as a robust system for wastewater treatment.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100145"},"PeriodicalIF":6.8,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10371416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm reactors for the treatment of used water in space:potential, challenges, and future perspectives","authors":"Erika J. Espinosa-Ortiz ,&nbsp;Robin Gerlach ,&nbsp;Brent M. Peyton ,&nbsp;Luke Roberson ,&nbsp;Daniel H. Yeh","doi":"10.1016/j.bioflm.2023.100140","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100140","url":null,"abstract":"<div><p>Water is not only essential to sustain life on Earth, but also is a crucial resource for long-duration deep space exploration and habitation. Current systems in space rely on the resupply of water from Earth, however, as missions get longer and move farther away from Earth, resupply will no longer be a sustainable option. Thus, the development of regenerative reclamation water systems through which useable water can be recovered from “waste streams” (<em>i.e.,</em> used waters) is sorely needed to further close the loop in space life support systems. This review presents the origin and characteristics of different used waters generated in space and discusses the intrinsic challenges of developing suitable technologies to treat such streams given the unique constrains of space exploration and habitation (<em>e.g.</em>, different gravity conditions, size and weight limitations, compatibility with other systems, <em>etc.</em>). In this review, we discuss the potential use of biological systems, particularly biofilms, as possible alternatives or additions to current technologies for water reclamation and waste treatment in space. The fundamentals of biofilm reactors, their advantages and disadvantages, as well as different reactor configurations and their potential for use and challenges to be incorporated in self-sustaining and regenerative life support systems in long-duration space missions are also discussed. <span>Furthermore</span>, we discuss the possibility to recover value-added products (<em>e.g.</em>, biomass, nutrients, water) from used waters and the opportunity to recycle and reuse such products as resources in other life support subsystems (<em>e.g.,</em> habitation, waste, air, <em>etc</em>.).</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100140"},"PeriodicalIF":6.8,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sucrose-mediated formation and adhesion strength of Streptococcus mutans biofilms on titanium","authors":"Laura J. Waldman ,&nbsp;Tony Butera ,&nbsp;James D. Boyd ,&nbsp;Martha E. Grady","doi":"10.1016/j.bioflm.2023.100143","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100143","url":null,"abstract":"<div><p>Biofilms consist of bacterial cells surrounded by a matrix of extracellular polymeric substance (EPS), which protects the colony from many countermeasures, including antibiotic treatments. Growth and formation of bacterial biofilms are affected by nutrients available in the environment. In the oral cavity, the presence of sucrose affects the growth of <em>Streptococcus mutans</em> that produce acids that erode enamel and form dental caries. Biofilm formation on dental implants commonly leads to severe infections and can restrict osseointegration necessary for the implant to be successful. This work determines the effect of sucrose concentration on biofilm EPS formation and adhesion of <em>Streptococcus mutans</em>, a common oral colonizer, to titanium substrates simulating common dental implants. Biofilm formation and profiles are visualized at high magnification with scanning electron microscopy (SEM). Large mounds and complex structures consisting of bacterial cells and EPS can be seen in biofilms at sucrose concentrations that are favorable for biofilm growth. The laser spallation technique is used to apply stress wave loading to the biofilm, causing the biofilm to delaminate at a critical tensile stress threshold. The critical tensile stress threshold is the adhesion strength. Because laser spallation applies the stress loading to the rear of the substrate, bulk adhesion properties of the biofilm can be determined despite the heterogenous composition and low cohesion strength of the biofilm. Statistical analysis reveals that adhesion strength of biofilms initially increase with increasing sucrose concentration and then decrease as sucrose concentration continues to increase. The adhesion strength of bacterial biofilms to the substrate in this study is compared to the adhesion of osteoblast-like cells to the same substrates published previously. When sucrose is present in the biofilm growth environment, <em>S. mutans</em> adhesion is higher than that of the osteoblast-like cells. Results of this study suggest sucrose-mediated <em>S. mutans</em> biofilms may outcompete osteoblasts in terms of adhesion during osseointegration, which could explain higher rates of peri-implant disease associated with high sugar diets. Further studies demonstrating adhesion differentials between biofilms and cells including co-cultures are needed and motivated by the present work.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100143"},"PeriodicalIF":6.8,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ex vivo comparison of V.A.C.® Granufoam Silver™ and V.A.C.® Granufoam™ loaded with a first-in-class bis-dialkylnorspermidine-terphenyl antibiofilm agent","authors":"Kaden B. Rawson ,&nbsp;Travis Neuberger ,&nbsp;Tyler B. Smith ,&nbsp;Isaac J. Bell ,&nbsp;Ryan E. Looper ,&nbsp;Paul R. Sebahar ,&nbsp;Travis J. Haussener ,&nbsp;Hariprasada Reddy Kanna Reddy ,&nbsp;Brad M. Isaacson ,&nbsp;John Shero ,&nbsp;Paul F. Pasquina ,&nbsp;Dustin L. Williams","doi":"10.1016/j.bioflm.2023.100142","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100142","url":null,"abstract":"<div><p>Implementation of negative pressure wound therapy (NPWT) as a standard of care has proven efficacious in reducing both the healing time and likelihood of nosocomial infection among pressure ulcers and traumatic, combat-related injuries. However, current formulations may not target or dramatically reduce bacterial biofilm burden following therapy. The purpose of this study was to determine the antibiofilm efficacy of an open-cell polyurethane (PU) foam (V.A.C.® Granufoam™) loaded with a first-in-class compound (CZ-01179) as the active release agent integrated via lyophilized hydrogel scaffolding. An <em>ex vivo</em> porcine excision wound model was designed to perform antibiofilm efficacy testing in the presence of NPWT. PU foam samples loaded with a 10.0% w/w formulation of CZ-01179 and 0.5% hyaluronic acid were prepared and tested against current standards of care: V.A.C.® Granufoam Silver™ and V.A.C.® Granufoam™. We observed statistically significant reduction of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) and <em>Acinetobacter baumannii</em> biofilms with the CZ-01179 antibiofilm foam in comparison to current standard of care foams. These findings motivate further development of an antibiofilm PU foam loaded with CZ-01179.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100142"},"PeriodicalIF":6.8,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}