Biofilm最新文献

{"title":"Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus","authors":"Xue Li ,&nbsp;Xiaobai Zhang ,&nbsp;Miaomiao Zhang ,&nbsp;Xi Luo ,&nbsp;Tingting Zhang ,&nbsp;Xianjin Liu ,&nbsp;Renfei Lu ,&nbsp;Yiquan Zhang","doi":"10.1016/j.bioflm.2024.100194","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100194","url":null,"abstract":"<div><p><em>Vibrio parahaemolyticus</em> is widely distributed in marine ecosystems. Magnesium ion (Mg²⁺) is the second most abundant metal cation in seawater, and plays important roles in the growth and gene expression of <em>V</em>. <em>parahaemolyticus</em>, but lacks the detailed mechanisms. In this study, the RNA sequencing data demonstrated that a total of 1494 genes was significantly regulated by Mg²⁺. The majority of the genes associated with lateral flagella, exopolysaccharide, type III secretion system 2, type VI secretion system (T6SS) 1, T6SS2, and thermostable direct hemolysin were downregulated. A total of 18 genes that may be involved in c-di-GMP metabolism and more than 80 genes encoding putative regulators were also significantly and differentially expressed in response to Mg²⁺, indicating that the adaptation process to Mg²⁺ stress may be strictly regulated by complex regulatory networks. In addition, Mg²⁺ promoted the proliferative speed, swimming motility and cell adhesion of <em>V. parahaemolyticus</em>, but inhibited the swarming motility, biofilm formation, and c-di-GMP production. However, Mg²⁺ had no effect on the production of capsular polysaccharide and cytoxicity against HeLa cells. Therefore, Mg²⁺ had a comprehensive impact on the physiology and gene expression of <em>V. parahaemolyticus</em>.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100194"},"PeriodicalIF":6.8,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000194/pdfft?md5=b295f021312469d61bab120357e131d9&pid=1-s2.0-S2590207524000194-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140330579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the development of cooperative anode-biofilm-structures","authors":"Edina Klein ,&nbsp;René Wurst ,&nbsp;David Rehnlund ,&nbsp;Johannes Gescher","doi":"10.1016/j.bioflm.2024.100193","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100193","url":null,"abstract":"<div><p>Microbial electrochemical systems are a highly versatile platform technology with a particular focus on the interplay of chemical and electrical energy conversion and offer immense potential for a sustainable bioeconomy. The industrial realization of this potential requires a critical focus on biofilm optimization if performance is to be controlled over a long period of time. Moreover, the aspect and influence of cooperativity has to be addressed as many applied anodic bioelectrochemical systems will most likely be operated with a diversity of interacting microbial species. Hence, the aim of this study was to analyze how interspecies dependence and cooperativity of a model community influence the development of anodic biofilms. To investigate biofilm activity in a spatially resolved manner, a microfluidic bioelectrochemical flow cell was developed that can be equipped with user-defined electrode materials and operates under laminar flow conditions. With this infrastructure, the development of single and co-culture biofilms of the two model organisms <em>Shewanella oneidensis</em> and <em>Geobacter sulfurreducens</em> on graphite electrodes was monitored by optical coherence tomography analysis. The interdependence in the co-culture biofilm was achieved by feeding the community with lactate, which is converted by <em>S. oneidensis</em> into acetate, which in turn serves as substrate for <em>G. sulfurreducens</em>. The results show that co-cultivation resulted in the formation of denser biofilms than in single culture. Moreover, we hypothesize that <em>S. oneidensis</em> in return utilizes the conductive biofilm matrix build by <em>G. sulfurreducens</em> for direct interspecies electron transfer (DIET) to the anode. FISH analysis revealed that the biofilms consisted of approximately two-thirds <em>G. sulfurreducens</em> cells, which most likely formed a conductive 3D network throughout the biofilm matrix, in which evenly distributed tubular <em>S. oneidensis</em> colonies were embedded without direct contact to the anode surface. Live/dead staining shows that the outermost biofilm contained almost exclusively dead cells (98 %), layers near the anode contained 45–56 % and the entire biofilm contained 82 % live cells. Our results exemplify how the architecture of the exoelectrogenic biofilm dynamically adapts to the respective process conditions.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100193"},"PeriodicalIF":6.8,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000182/pdfft?md5=f7579a7a5626990c87322a47e6c0fbae&pid=1-s2.0-S2590207524000182-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140350389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoparticles synthesized from Pseudomonas aeruginosa pyoverdine: Antibiofilm and antivirulence agents","authors":"Nazia Tabassum ,&nbsp;Fazlurrahman Khan ,&nbsp;Geum-Jae Jeong ,&nbsp;Du-Min Jo ,&nbsp;Young-Mog Kim","doi":"10.1016/j.bioflm.2024.100192","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100192","url":null,"abstract":"<div><p>The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one of the major issues in the healthcare sector. In the present study, nanomaterial-based controlling the biofilm and virulence properties has been considered an alternative approach. Pyoverdine (PVD) isolated from the <em>Pseudomonas aeruginosa</em> was utilized as a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful in a targeted action to microbial pathogens due to the recognition of the corona of the nanoparticles by the pathogenic membrane. Synthesized PVD-AgNPs were spherical to irregular, with an average size value of 251.87 ± 21.8 nm and zeta potential with a value of −36.51 ± 0.69 mV. The MIC value of PVD-AgNPs towards <em>P. aeruginosa</em>, <em>Listeria monocytogenes</em>, <em>Staphylococcus aureus</em>, <em>Streptococcus mutans</em>, <em>Escherichia coli</em>, and <em>Candida albicans</em> in the standard and host-mimicking media were observed in decreasing order in a multi-fold, such as standard growth media &gt; sputum &gt; synthetic human urine &gt; saliva. Both the initial stage and the well-established biofilms of these microbial pathogens have been effectively inhibited and eradicated by PVD-AgNPs. PVD-AgNPs increase the susceptibility of tetracycline, PVD, and amphotericin B towards established mature mono- and mixed-species biofilms of <em>S. aureus</em> and <em>C. albicans</em>. Additionally, PVD-AgNPs attenuate several virulence properties, such as inhibition of protease activity, motility, and PVD and pyocyanin production in <em>P. aeruginosa</em>. The inhibition of gene expression of biofilm and virulence-associated genes in <em>P. aeruginosa</em> validates its phenotypic effects.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100192"},"PeriodicalIF":6.8,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000170/pdfft?md5=ed142768e733aae0e35a68f8769b2249&pid=1-s2.0-S2590207524000170-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140162988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"cmpX overexpression in Pseudomonas aeruginosa affects biofilm formation and cell morphology in response to shear stress","authors":"Audrey David ,&nbsp;Mélissande Louis ,&nbsp;Ali Tahrioui ,&nbsp;Sophie Rodrigues ,&nbsp;Clarisse Labbé ,&nbsp;Olivier Maillot ,&nbsp;Magalie Barreau ,&nbsp;Olivier Lesouhaitier ,&nbsp;Pierre Cornelis ,&nbsp;Sylvie Chevalier ,&nbsp;Emeline Bouffartigues","doi":"10.1016/j.bioflm.2024.100191","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100191","url":null,"abstract":"<div><p><em>Pseudomonas aeruginosa</em> is an opportunistic pathogen causing chronic infections that are related to its ability to form biofilms. Mechanosensitive ion channels (Mcs) are cytoplasmic membrane proteins whose opening depends on a mechanical stress impacting the lipid bilayer. CmpX is a homologue of the small conductance MscS of <em>Escherichia coli</em>. The <em>cmpX</em> gene is part of a transcriptional <em>cfrX-cmpX</em> unit that is under the control of the cell envelope stress response ECF sigma factor SigX. CmpX was shown to regulate the activity of the hybrid sensor kinase PA1611 involved in the regulation of transition from a planktonic to a biofilm lifestyle. The deletion of <em>cmpX</em> leads to increased biofilm formation under static conditions. Herein, the effect of <em>cmpX</em> overexpression was investigated by confocal laser scanning microscopy in terms of biofilm formation and architecture, and matrix components production, in dynamic conditions. We show that overexpression of <em>cmpX</em> in <em>P. aeruginosa</em> leads to enhanced and altered biofilm architecture that seems to be associated to increased matrix components and the emergence of filamentous cells. These phenotypic alterations might occur potentially through a shear stress induced by the medium flow rate.</p></div><div><h3>Importance</h3><p>CmpX is involved in biofilm formation and cell filamentation with regards to the medium flow.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100191"},"PeriodicalIF":6.8,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000169/pdfft?md5=824802a5c37d46f46940b17da41b5ed2&pid=1-s2.0-S2590207524000169-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140160786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic avian mycoplasmas show phenotypic differences in their biofilm forming ability compared to non-pathogenic species in vitro","authors":"Salvatore Catania ,&nbsp;Marco Bottinelli ,&nbsp;Alice Fincato ,&nbsp;Annalucia Tondo ,&nbsp;Andrea Matucci ,&nbsp;Giorgia Nai ,&nbsp;Verdiana Righetti ,&nbsp;Francesco Abbate ,&nbsp;Ana S. Ramírez ,&nbsp;Federica Gobbo ,&nbsp;Marianna Merenda","doi":"10.1016/j.bioflm.2024.100190","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100190","url":null,"abstract":"<div><p>Mycoplasmas are known as the minimalist microorganisms in the microbes’ world. Their minimalist nature makes them highly sensitive to the environmental conditions and limits their ability to survive for extended periods outside their animal host. Nevertheless, there are documented instances of mycoplasma transmission over significant distances and this phenomenon may be linked to relatively unexplored abilities of mycoplasmas, such as their capacity to synthesize biofilm—the predominant mode of bacterial growth in nature. The authors decided to establish a method aimed at inducing the clustering of mycoplasma planktonic cells within a biofilm <em>in vitro</em> and subsequently assess the capacity of certain avian mycoplasmas to synthesize a biofilm. A total of 299 avian mycoplasma isolates were included in the study, encompassing both pathogenic (<em>Mycoplasma gallisepticum</em>, <em>M. synoviae</em>, <em>M. meleagridis</em>, <em>M. iowae</em>) and non-pathogenic species (<em>M. gallinaceum</em>, <em>M. gallinarum</em>, <em>M. iners</em> and <em>M. pullorum)</em>. The authors successfully demonstrated the feasibility of inducing avian mycoplasmas to synthetize <em>in vitro</em> a biofilm, which can be visually quantified. The only species that did not produce any biofilm was <em>M. iowae</em>. In general, the pathogenic mycoplasmas produced greater quantities of biofilm compared to the non-pathogenic ones. Furthermore, it was observed that the ability to produce biofilm appeared to vary, both qualitatively and quantitatively, not only among different species but also among isolates of a single species. Future studies will be necessary to determine whether biofilm production plays a pivotal epidemiological role for the pathogenic avian mycoplasmas.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100190"},"PeriodicalIF":6.8,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000157/pdfft?md5=416919fe0e92b4d2df0ea5758a570080&pid=1-s2.0-S2590207524000157-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140134162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A normalized parameter for comparison of biofilm dispersants in vitro","authors":"Shuang Tian ,&nbsp;Linqi Shi ,&nbsp;Yijin Ren ,&nbsp;Henny C. van der Mei ,&nbsp;Henk J. Busscher","doi":"10.1016/j.bioflm.2024.100188","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100188","url":null,"abstract":"<div><p>Dispersal of infectious biofilms increases bacterial concentrations in blood. To prevent sepsis, the strength of a dispersant should be limited to allow the immune system to remove dispersed bacteria from blood, preferably without antibiotic administration. Biofilm bacteria are held together by extracellular polymeric substances that can be degraded by dispersants. Currently, comparison of the strength of dispersants is not possible by lack of a suitable comparison parameter. Here, a biofilm dispersal parameter is proposed that accounts for differences in initial biofilm properties, dispersant concentration and exposure time by using PBS as a control and normalizing outcomes with respect to concentration and time. The parameter yielded near-identical values based on dispersant-induced reductions in biomass or biofilm colony-forming-units and appeared strain-dependent across pathogens. The parameter as proposed is largely independent of experimental methods and conditions and suitable for comparing different dispersants with respect to different causative strains in particular types of infection.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100188"},"PeriodicalIF":6.8,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000133/pdfft?md5=c72c8fc107d3d2c9b9ce5030de4009a2&pid=1-s2.0-S2590207524000133-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140063130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of rifampicin combination therapy against MRSA prosthetic vascular graft infections in a rat model","authors":"Mikkel Illemann Johansen ,&nbsp;Maiken Engelbrecht Petersen ,&nbsp;Emma Faddy ,&nbsp;Anders Marthinsen Seefeldt ,&nbsp;Alexander Alexandrovich Mitkin ,&nbsp;Lars Østergaard ,&nbsp;Rikke Louise Meyer ,&nbsp;Nis Pedersen Jørgensen","doi":"10.1016/j.bioflm.2024.100189","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100189","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> is a major cause of prosthetic vascular graft or endograft infections (VGEIs) and the optimal choice of antibiotics is unclear. We investigated various antibiotic choices as either monotherapy or combination therapy with rifampicin against MRSA <em>in vitro</em> and <em>in vivo</em>.</p><p>Fosfomycin, daptomycin and vancomycin alone or in combination with rifampicin was used against MRSA USA300 FPR3757. Each antibiotic was tested for synergism or antagonism with rifampicin <em>in vitro,</em> and all antibiotic regimens were tested against actively growing bacteria in media and non-growing bacteria in buffer, both as planktonic cells and in biofilms. A rat model of VGEI was used to quantify the therapeutic efficacy of antibiotics <em>in vivo</em> by measuring bacterial load on grafts and in spleen, liver and kidneys.</p><p><em>In vitro,</em> rifampicin combinations did not reveal any synergism or antagonism in relation to growth inhibition. However, quantification of bactericidal activity revealed a strong antagonistic effect, both on biofilms and planktonic cells. This effect was only observed when treating active bacteria, as all antibiotics had little or no effect on inactive cells. Only daptomycin showed some biocidal activity against inactive cells. <em>In vivo</em> evaluation of therapy against VGEI contrasted the <em>in vitro</em> results<em>.</em> Rifampicin significantly increased the efficacy of both daptomycin and vancomycin. The combination of daptomycin and rifampicin was by far the most effective, curing 8 of 13 infected animals.</p><p>Our study demonstrates that daptomycin in combination with rifampicin shows promising potential against VGEI caused by MRSA. Furthermore, we show how <em>in vitro</em> evaluation of antibiotic combinations in laboratory media does not predict their therapeutic effect against VGEI <em>in vivo</em>, presumably due to a difference in the metabolic state of the bacteria.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100189"},"PeriodicalIF":6.8,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000145/pdfft?md5=e95db5a749838693e3087c7d7413e390&pid=1-s2.0-S2590207524000145-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV emitting glass: A promising strategy for biofilm inhibition on transparent surfaces","authors":"Leila Alidokht ,&nbsp;Katrina Fitzpatrick ,&nbsp;Caitlyn Butler ,&nbsp;Kelli Z. Hunsucker ,&nbsp;Cierra Braga ,&nbsp;William A. Maza ,&nbsp;Kenan P. Fears ,&nbsp;Marieh Arekhi ,&nbsp;Mariana Lanzarini-Lopes","doi":"10.1016/j.bioflm.2024.100186","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100186","url":null,"abstract":"<div><p>Marine biofouling causes serious environmental problems and has adverse impacts on the maritime industry. Biofouling on windows and optical equipment reduces surface transparency, limiting their application for on-site monitoring or continuous measurement. This work illustrates that UV emitting glasses (UEGs) can prevent the establishment and growth of biofilm on the illuminated surfaces. Specifically, this paper describes how UEGs are enabled by innovatively modifying the surfaces of the glass with light scattering particles. Modification of glass surface with silica nanoparticles at a concentration 26.5 μg/cm² resulted in over ten-fold increase in UV irradiance, while maintaining satisfactory visible and IR transparency metrics of over 99 %. The UEG reduced visible biological growth by 98 % and resulted in a decrease of 1.79 log in detected colony forming units when compared to the control during a 20 day submersion at Port Canaveral, Florida, United States. These findings serve as strong evidence that UV emitting glass should be explored as a promising approach for biofilm inhibition on transparent surfaces.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100186"},"PeriodicalIF":6.8,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259020752400011X/pdfft?md5=e4deb4ca639f1e52c5c299dc254470d1&pid=1-s2.0-S259020752400011X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140069468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical analysis of methods to determine growth, control and analysis of biofilms for potential non-submerged antibiofilm surfaces and coatings","authors":"J. Redfern ,&nbsp;A.J. Cunliffe ,&nbsp;D.M. Goeres ,&nbsp;N.F. Azevedo ,&nbsp;J. Verran","doi":"10.1016/j.bioflm.2024.100187","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100187","url":null,"abstract":"<div><p>The potential uses for antibiofilm surfaces reach across different sectors with significant resultant economic, societal and health impact. For those interested in using antibiofilm surfaces in the built environment, it is important that efficacy testing methods are relevant, reproducible and standardised where possible, to ensure data outputs are applicable to end-use, and comparable across the literature. Using pre-defined keywords, a review of literature reporting on antimicrobial surfaces (78 articles), within which a potential application was described as non-submerged/non-medical surface or coating with antibiofilm action, was undertaken. The most used methods utilized the growth of biofilm in submerged and static systems. Quantification varied (from most to least commonly used) across colony forming unit counts, non-microscopy fluorescence or spectroscopy, microscopy analysis, direct agar-contact, sequencing, and ELISA. Selection of growth media, microbial species, and incubation temperature also varied. In many cases, definitions of biofilm and attempts to quantify antibiofilm activity were absent or vague. Assessing a surface after biofilm recovery or assessing potential regrowth of a biofilm after initial analysis was almost entirely absent. It is clear the field would benefit from widely agreed and adopted approaches or guidance on how to select and incorporate end-use specific conditions, alongside minimum reporting guidelines may benefit the literature.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100187"},"PeriodicalIF":6.8,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000121/pdfft?md5=a01ed8c7a2d67bcedbaee54280da22ab&pid=1-s2.0-S2590207524000121-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of biomimetic surfaces to reduce single- and dual-species biofilms of Escherichia coli and Pseudomonas putida","authors":"Rita Teixeira-Santos ,&nbsp;Ana Azevedo ,&nbsp;Maria J. Romeu ,&nbsp;Cristina I. Amador ,&nbsp;Luciana C. Gomes ,&nbsp;Kathryn A. Whitehead ,&nbsp;Jelmer Sjollema ,&nbsp;Mette Burmølle ,&nbsp;Filipe J. Mergulhão","doi":"10.1016/j.bioflm.2024.100185","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100185","url":null,"abstract":"<div><p>The ability of bacteria to adhere to and form biofilms on food contact surfaces poses serious challenges, as these may lead to the cross-contamination of food products. Biomimetic topographic surface modifications have been explored to enhance the antifouling performance of materials. In this study, the topography of two plant leaves, <em>Brassica oleracea</em> var. <em>botrytis</em> (cauliflower, CF) and <em>Brassica oleracea capitate</em> (white cabbage, WC), was replicated through wax moulding, and their antibiofilm potential was tested against single- and dual-species biofilms of <em>Escherichia coli</em> and <em>Pseudomonas putida</em>. Biomimetic surfaces exhibited higher roughness values (<em>S</em>_<em>a</em> _WC = 4.0 ± 1.0 μm and <em>S</em>_<em>a</em> _CF = 3.3 ± 1.0 μm) than the flat control (<em>S</em>_<em>a</em> _F = 0.6 ± 0.2 μm), whilst the CF surface demonstrated a lower interfacial free energy (<em>ΔG</em>_<em>iwi</em>) than the WC surface (−100.08 mJ m⁻² and −71.98 mJ m⁻², respectively). The CF and WC surfaces had similar antibiofilm effects against single-species biofilms, achieving cell reductions of approximately 50% and 60% for <em>E. coli</em> and <em>P. putida</em>, respectively, compared to the control. Additionally, the biomimetic surfaces led to reductions of up to 60% in biovolume, 45% in thickness, and 60% in the surface coverage of single-species biofilms. For dual-species biofilms, only the <em>E. coli</em> strain growing on the WC surface exhibited a significant decrease in the cell count. However, confocal microscopy analysis revealed a 60% reduction in the total biovolume and surface coverage of mixed biofilms developed on both biomimetic surfaces. Furthermore, dual-species biofilms were mainly composed of <em>P. putida</em>, which reduced <em>E. coli</em> growth. Altogether, these results demonstrate that the surface properties of CF and WC biomimetic surfaces have the potential for reducing biofilm formation.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100185"},"PeriodicalIF":6.8,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000108/pdfft?md5=23e4ee41bb43669ceb9cc195da841e72&pid=1-s2.0-S2590207524000108-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}