Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus

Abstract

Vibrio parahaemolyticus is widely distributed in marine ecosystems. Magnesium ion (Mg²⁺) is the second most abundant metal cation in seawater, and plays important roles in the growth and gene expression of V. parahaemolyticus, but lacks the detailed mechanisms. In this study, the RNA sequencing data demonstrated that a total of 1494 genes was significantly regulated by Mg²⁺. The majority of the genes associated with lateral flagella, exopolysaccharide, type III secretion system 2, type VI secretion system (T6SS) 1, T6SS2, and thermostable direct hemolysin were downregulated. A total of 18 genes that may be involved in c-di-GMP metabolism and more than 80 genes encoding putative regulators were also significantly and differentially expressed in response to Mg²⁺, indicating that the adaptation process to Mg²⁺ stress may be strictly regulated by complex regulatory networks. In addition, Mg²⁺ promoted the proliferative speed, swimming motility and cell adhesion of V. parahaemolyticus, but inhibited the swarming motility, biofilm formation, and c-di-GMP production. However, Mg²⁺ had no effect on the production of capsular polysaccharide and cytoxicity against HeLa cells. Therefore, Mg²⁺ had a comprehensive impact on the physiology and gene expression of V. parahaemolyticus.