Biofilm最新文献

{"title":"Dual roles of the conditional extracellular vesicles derived from Pseudomonas aeruginosa biofilms: Promoting and inhibiting bacterial biofilm growth","authors":"Marwa Gamal Saad,&nbsp;Haluk Beyenal,&nbsp;Wen-Ji Dong","doi":"10.1016/j.bioflm.2024.100183","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100183","url":null,"abstract":"<div><p>Antibiotic-resistant biofilm infections have emerged as public health concerns because of their enhanced tolerance to high-dose antibiotic treatments. The biofilm life cycle involves multiple developmental stages, which are tightly regulated by active cell-cell communication via specific extracellular signal messengers such as extracellular vesicles. This study was aimed at exploring the roles of extracellular vesicles secreted by <em>Pseudomonas aeruginosa</em> at different developmental stages in controlling biofilm growth. Our results show that extracellular vesicles secreted by <em>P. aeruginosa</em> biofilms during their exponential growth phase (G-EVs) enhance biofilm growth. In contrast, extracellular vesicles secreted by <em>P. aeruginosa</em> biofilms during their death/survival phase (D-EVs) can effectively inhibit/eliminate <em>P. aeruginosa</em> PAO1 biofilms up to 4.8-log₁₀ CFU/cm². The inhibition effectiveness of D-EVs against <em>P. aeruginosa</em> biofilms grown for 96 h improved further in the presence of 10–50 μM Fe³⁺ ions. Proteomic analysis suggests the inhibition involves an iron-dependent ferroptosis mechanism. This study is the first to report the functional role of bacterial extracellular vesicles in bacterial growth, which depends on the developmental stage of the parent bacteria. The finding of D-EV-activated ferroptosis-based bacterial death may have significant implications for preventing antibiotic resistance in biofilms.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100183"},"PeriodicalIF":6.8,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259020752400008X/pdfft?md5=159d0d8522a5a1c09e88ea8c53c7b493&pid=1-s2.0-S259020752400008X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139726151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Space biofilms – An overview of the morphology of Pseudomonas aeruginosa biofilms grown on silicone and cellulose membranes on board the international space station","authors":"Pamela Flores ,&nbsp;Jiaqi Luo ,&nbsp;Daniel Wyn Mueller ,&nbsp;Frank Muecklich ,&nbsp;Luis Zea","doi":"10.1016/j.bioflm.2024.100182","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100182","url":null,"abstract":"<div><p>Microorganisms’ natural ability to live as organized multicellular communities – also known as biofilms – provides them with unique survival advantages. For instance, bacterial biofilms are protected against environmental stresses thanks to their extracellular matrix, which could contribute to persistent infections after treatment with antibiotics. Bacterial biofilms are also capable of strongly attaching to surfaces, where their metabolic by-products could lead to surface material degradation. Furthermore, microgravity can alter biofilm behavior in unexpected ways, making the presence of biofilms in space a risk for both astronauts and spaceflight hardware. Despite the efforts to eliminate microorganism contamination from spacecraft surfaces, it is impossible to prevent human-associated bacteria from eventually establishing biofilm surface colonization. Nevertheless, by understanding the changes that bacterial biofilms undergo in microgravity, it is possible to identify key differences and pathways that could be targeted to significantly reduce biofilm formation. The bacterial component of Space Biofilms project, performed on the International Space Station in early 2020, contributes to such understanding by characterizing the morphology and gene expression of bacterial biofilms formed in microgravity with respect to ground controls. <em>Pseudomonas aeruginosa</em> was used as model organism due to its relevance in biofilm studies and its ability to cause urinary tract infections as an opportunistic pathogen. Biofilm formation was characterized at one, two, and three days of incubation (37 °C) over six different materials. Materials reported in this manuscript include catheter grade silicone, selected due to its medical relevance in hospital acquired infections, catheter grade silicone with ultrashort pulsed direct laser interference patterning, included to test microtopographies as a potential biofilm control strategy, and cellulose membrane to replicate the column and canopy structure previously reported from a microgravity study. We here present an overview of the biofilm morphology, including 3D images of the biofilms to represent the distinctive morphology observed in each material tested, and some of the key differences in biofilm thickness, mass, and surface area coverage. We also present the impact of the surface microtopography in biofilm formation across materials, incubation time, and gravitational conditions.</p><p>The Space Biofilms project (bacterial side) is supported by the <span>National Aeronautics and Space Administration</span> under Grant No. 80NSSC17K0036 and 80NSSC21K1950.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100182"},"PeriodicalIF":6.8,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000078/pdfft?md5=c2d605d4c37c6e75fd4d5159a94909d9&pid=1-s2.0-S2590207524000078-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139719592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant protein production in Pseudoalteromonas haloplanktis TAC125 biofilm","authors":"Marzia Calvanese ,&nbsp;Caterina D'Angelo ,&nbsp;Concetta Lauro ,&nbsp;Maria Luisa Tutino ,&nbsp;Ermenegilda Parrilli","doi":"10.1016/j.bioflm.2024.100179","DOIUrl":"10.1016/j.bioflm.2024.100179","url":null,"abstract":"<div><p>Biofilms have great potential for producing valuable products, and recent research has been performed on biofilms for the production of compounds with biotechnological and industrial relevance. However, the production of recombinant proteins using this system is still limited. The recombinant protein production in microbial hosts is a well-established technology and a variety of expression systems are available. Nevertheless, the production of some recombinant proteins can result in proteolyzed, insoluble, and non-functional forms, therefore it is necessary to start the exploration of non-conventional production systems that, in the future, could be helpful to produce some “difficult” proteins. Non-conventional production systems can be based on the use of alternative hosts and/or on non-conventional ways to grow recombinant cells. In this paper, the use of the Antarctic marine bacterium <em>Pseudoalteromonas haloplanktis</em> TAC125 grown in biofilm conditions was explored to produce two fluorescent proteins, GFP and mScarlet. The best conditions for the production were identified by working on media composition, and induction conditions, and by building a new expression vector suitable for the biofilm conditions. Results reported demonstrated that the optimized system for the recombinant protein production in biofilm, although it takes longer than planktonic production, has the same potentiality as the classical planktonic approach with additional advantages since it needs a lower concentration of the carbon sources and doesn't require antibiotic addition. Moreover, in the case of mScarlet, the production in biofilm outperforms the planktonic system in terms of a better quality of the recombinant product.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100179"},"PeriodicalIF":6.8,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000042/pdfft?md5=5ffc584d4ab994c41eca6d9c81d0fe45&pid=1-s2.0-S2590207524000042-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139638062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal evaluation of drug antibacterial activity reveals cinnamaldehyde analog anti-biofilm effects against Haemophilus influenzae","authors":"Javier Asensio-López ,&nbsp;María Lázaro-Díez ,&nbsp;Tania M. Hernández-Cruz ,&nbsp;Núria Blanco-Cabra ,&nbsp;Ioritz Sorzabal-Bellido ,&nbsp;Eva M. Arroyo-Urea ,&nbsp;Elena Buetas ,&nbsp;Ana González-Paredes ,&nbsp;Carlos Ortiz de Solórzano ,&nbsp;Saioa Burgui ,&nbsp;Eduard Torrents ,&nbsp;María Monteserín ,&nbsp;Junkal Garmendia","doi":"10.1016/j.bioflm.2024.100178","DOIUrl":"10.1016/j.bioflm.2024.100178","url":null,"abstract":"<div><p>Biofilm formation by the pathobiont <em>Haemophilus influenzae</em> is associated with human nasopharynx colonization, otitis media in children, and chronic respiratory infections in adults suffering from chronic respiratory diseases such as chronic obstructive pulmonary disease (COPD). β-lactam and quinolone antibiotics are commonly used to treat these infections. However, considering the resistance of biofilm-resident bacteria to antibiotic-mediated killing, the use of antibiotics may be insufficient and require being replaced or complemented with novel strategies. Moreover, unlike the standard minimal inhibitory concentration assay used to assess antibacterial activity against planktonic cells, standardization of methods to evaluate anti-biofilm drug activity is limited. In this work, we detail a panel of protocols for systematic analysis of drug antimicrobial effect on bacterial biofilms, customized to evaluate drug effects against <em>H. influenzae</em> biofilms. Testing of two cinnamaldehyde analogs, (<em>E</em>)-trans-2-nonenal and (<em>E</em>)-3-decen-2-one, demonstrated their effectiveness in both <em>H. influenzae</em> inhibition of biofilm formation and eradication or preformed biofilms. Assay complementarity allowed quantifying the dynamics and extent of the inhibitory effects, also observed for ampicillin resistant clinical strains forming biofilms refractory to this antibiotic. Moreover, cinnamaldehyde analog encapsulation into poly(lactic-<em>co</em>-glycolic acid) (PLGA) polymeric nanoparticles allowed drug vehiculization while maintaining efficacy. Overall, we demonstrate the usefulness of cinnamaldehyde analogs against <em>H. influenzae</em> biofilms, present a test panel that can be easily adapted to a wide range of pathogens and drugs, and highlight the benefits of drug nanoencapsulation towards safe controlled release.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100178"},"PeriodicalIF":6.8,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000030/pdfft?md5=17c58e04e417eb43a680ed6a84da5a57&pid=1-s2.0-S2590207524000030-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-species biofilms of environmental microbiota isolated from fruit packing facilities promoted tolerance of Listeria monocytogenes to benzalkonium chloride","authors":"M. Laura Rolon ,&nbsp;Olena Voloshchuk ,&nbsp;Katelyn V. Bartlett ,&nbsp;Luke F. LaBorde ,&nbsp;Jasna Kovac","doi":"10.1016/j.bioflm.2024.100177","DOIUrl":"https://doi.org/10.1016/j.bioflm.2024.100177","url":null,"abstract":"<div><p><em>Listeria monocytogenes</em> may survive and persist in food processing environments due to formation of complex multi-species biofilms of environmental microbiota that co-exists in these environments. This study aimed to determine the effect of selected environmental microbiota on biofilm formation and tolerance of <em>L. monocytogenes</em> to benzalkonium chloride in formed biofilms. The studied microbiota included bacterial families previously shown to co-occur with <em>L. monocytogenes</em> in tree fruit packing facilities, including <em>Pseudomonadaceae, Xanthomonadaceae, Microbacteriaceae</em>, and <em>Flavobacteriaceae.</em> Biofilm formation ability and the effect of formed biofilms on the tolerance of <em>L. monocytogenes</em> to benzalkonium chloride was measured in single- and multi-family assemblages. Biofilms were grown statically on polystyrene pegs submerged in a R2A broth. Biofilm formation was quantified using a crystal violet assay, spread-plating, confocal laser scanning microscopy, and its composition was assessed using amplicon sequencing. The concentration of <em>L. monocytogenes</em> in biofilms was determined using the most probable number method. Biofilms were exposed to the sanitizer benzalkonium chloride, and the death kinetics of <em>L. monocytogenes</em> were quantified using a most probable number method. A total of 8, 8, 6, and 3 strains of <em>Pseudomonadaceae, Xanthomonadaceae, Microbacteriaceae</em>, and <em>Flavobacteriaceae</em>, respectively, were isolated from the environmental microbiota of tree fruit packing facilities and were used in this study. Biofilms formed by <em>Pseudomonadaceae, Xanthomonadaceae</em>, and all multi-family assemblages had significantly higher concentration of bacteria, as well as <em>L. monocytogenes,</em> compared to biofilms formed by <em>L. monocytogenes</em> alone. Furthermore, multi-family assemblage biofilms increased the tolerance of <em>L. monocytogenes</em> to benzalkonium chloride compared to <em>L. monocytogenes</em> mono-species biofilms and planktonic multi-family assemblages.</p><p>These findings suggest that <em>L. monocytogenes</em> control strategies should focus not only on assessing the efficacy of sanitizers against <em>L. monocytogenes,</em> but also against biofilm-forming microorganisms that reside in the food processing built environment, such as <em>Pseudomonadaceae</em> or <em>Xanthomonadaceae</em>.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100177"},"PeriodicalIF":6.8,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000029/pdfft?md5=bd7100c2d3cc75c253f70bf8a0353113&pid=1-s2.0-S2590207524000029-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139549364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating a polymicrobial biofilm model for structural components by co-culturing Komagataeibacter hansenii produced bacterial cellulose with Pseudomonas aeruginosa PAO1","authors":"Usha Rani Mahadevaswamy ,&nbsp;Sudarsan Mugunthan ,&nbsp;Thomas Seviour ,&nbsp;Staffan Kjelleberg ,&nbsp;Sierin Lim","doi":"10.1016/j.bioflm.2024.100176","DOIUrl":"10.1016/j.bioflm.2024.100176","url":null,"abstract":"<div><p>A polymicrobial biofilm model of <em>Komagataeibacter hansenii</em> and <em>Pseudomonas aeruginosa</em> was developed to understand whether a pre-existing matrix affects the ability of another species to build a biofilm. <em>P. aeruginosa</em> was inoculated onto the preformed <em>K. hansenii</em> biofilm consisting of a cellulose matrix. <em>P. aeruginosa</em> PAO1 colonized and infiltrated the <em>K. hansenii</em> bacterial cellulose biofilm (BC), as indicated by the presence of cells at 19 μm depth in the translucent hydrogel matrix. Bacterial cell density increased along the imaged depth of the biofilm (17-19 μm). On day 5, the average bacterial count across sections was 67 ± 4 % <em>P</em>. <em>aeruginosa</em> PAO1 and 33 ± 6 % <em>K. hansenii</em>. Biophysical characterization of the biofilm indicated that colonization by <em>P. aeruginosa</em> modified the biophysical properties of the BC matrix, which inlcuded increased density, heterogeneity, degradation temperature and thermal stability, and reduced crystallinity, swelling ability and moisture content. This further indicates colonization of the biofilm by <em>P. aeruginosa.</em> While eDNA fibres - a key viscoelastic component of <em>P. aeruginosa</em> biofilm - were present on the surface of the co-cultured biofilm on day 1, their abundance decreased over time, and by day 5, no eDNA was observed, either on the surface or within the matrix. <em>P. aeruginosa</em>-colonized biofilm devoid of eDNA retained its mechanical properties. The observations demonstrate that a pre-existing biofilm scaffold of <em>K. hansenii</em> inhibits <em>P. aeruginosa</em> PAO1 eDNA production and suggest that eDNA production is a response by <em>P. aeruginosa</em> to the viscoelastic properties of its environment.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100176"},"PeriodicalIF":6.8,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000017/pdfft?md5=40722a72395d4bc5bddc6111baf065e3&pid=1-s2.0-S2590207524000017-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139396045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics","authors":"Xiying Wu ,&nbsp;Huan Wang ,&nbsp;Juan Xiong ,&nbsp;Guo-Xun Yang ,&nbsp;Jin-Feng Hu ,&nbsp;Quangang Zhu ,&nbsp;Zhongjian Chen","doi":"10.1016/j.bioflm.2023.100175","DOIUrl":"10.1016/j.bioflm.2023.100175","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> can readily form biofilm which enhances the drug-resistance, resulting in life-threatening infections involving different organs. Biofilm formation occurs due to a series of developmental events including bacterial adhesion, aggregation, biofilm maturation, and dispersion, which are controlled by multiple regulatory systems. Rapidly increasing research and development outcomes on natural products targeting <em>S. aureus</em> biofilm formation and/or regulation led to an emergent application of active phytochemicals and combinations. This review aimed at providing an in-depth understanding of biofilm formation and regulation mechanisms for <em>S. aureus</em>, outlining the most important antibiofilm strategies and potential targets of natural products, and summarizing the latest progress in combating <em>S. aureus</em> biofilm with plant-derived natural products. These findings provided further evidence for novel antibiofilm drugs research and clinical therapies.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100175"},"PeriodicalIF":6.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000722/pdfft?md5=de95160fca89faa521330c2223df4f04&pid=1-s2.0-S2590207523000722-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of DNase inhibition of early biofilm formation Pseudomonas aeruginosa or Staphylococcus aureus: A transcriptome analysis","authors":"Wusheng Deng ,&nbsp;Chuanlin Zhou ,&nbsp;Jiaoxia Qin ,&nbsp;Yun Jiang ,&nbsp;Dingbin Li ,&nbsp;Xiujia Tang ,&nbsp;Jing Luo ,&nbsp;Jinliang Kong ,&nbsp;Ke Wang","doi":"10.1016/j.bioflm.2023.100174","DOIUrl":"10.1016/j.bioflm.2023.100174","url":null,"abstract":"<div><p><em>In vitro</em> studies show that DNase can inhibit <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> biofilm formation. However, the underlying molecular mechanisms remain poorly understood. This study used an RNA-sequencing transcriptomic approach to investigate the mechanism by which DNase I inhibits early <em>P. aeruginosa</em> and <em>S. aureus</em> biofilm formation on a transcriptional level, respectively. A total of 1171 differentially expressed genes (DEGs) in <em>P. aeruginosa</em> and 1016 DEGs in <em>S. aureus</em> enriched in a variety of biological processes and pathways were identified, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the DEGs were primarily involved in <em>P. aeruginosa</em> two-component system, biofilm formation, and flagellar assembly and in <em>S. aureus</em> biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and biosynthesis of amino acids, respectively. The transcriptional data were validated using quantitative real-time polymerase chain reaction (RT-qPCR), and the expression profiles of 22 major genes remained consistent. These findings suggested that DNase I may inhibit early biofilm formation by downregulating the expression of <em>P. aeruginosa</em> genes associated with flagellar assembly and the type VI secretion system, and by downregulating <em>S. aureus</em> capsular polysaccharide and amino acids metabolism gene expression, respectively. This study offers insights into the mechanisms of DNase treatment-based inhibition of early <em>P. aeruginosa</em> and <em>S. aureus</em> biofilm formation.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100174"},"PeriodicalIF":6.8,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000710/pdfft?md5=74589b71e11946c4375f79d94cc5af0a&pid=1-s2.0-S2590207523000710-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139190125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling of foodborne pathogen biofilms on stainless steel by bacteriophages: A systematic review and meta-analysis","authors":"Rahim Azari ,&nbsp;Mohammad Hashem Yousefi ,&nbsp;Aziz A. Fallah ,&nbsp;Arezoo Alimohammadi ,&nbsp;Nastaran Nikjoo ,&nbsp;Jeroen Wagemans ,&nbsp;Enayat Berizi ,&nbsp;Saeid Hosseinzadeh ,&nbsp;Mohammad Ghasemi ,&nbsp;Amin Mousavi Khaneghah","doi":"10.1016/j.bioflm.2023.100170","DOIUrl":"10.1016/j.bioflm.2023.100170","url":null,"abstract":"<div><p>This study investigates the potential of using bacteriophages to control foodborne pathogen biofilms on stainless steel surfaces in the food industry. Biofilm-forming bacteria can attach to stainless steel surfaces, rendering them difficult to eradicate even after a thorough cleaning and sanitizing procedures. Bacteriophages have been proposed as a possible solution, as they can penetrate biofilms and destroy bacterial cells within, reducing the number of viable bacteria and preventing the growth and spread of biofilms. This systematic review and meta-analysis evaluates the potential of bacteriophages against different biofilm-forming foodborne bacteria, including <em>Cronobacter sakazakii</em>, <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, <em>Pseudomonas fluorescens</em>, <em>Pseudomonas aeruginosa</em> and <em>Listeria monocytogenes</em>. Bacteriophage treatment generally causes a significant average reduction of 38 % in biofilm formation of foodborne pathogens on stainless steel. Subgroup analyses revealed that phages are more efficient in long-duration treatment. Also, applying a cocktail of phages is 1.26-fold more effective than applying individual phages. Phages at concentrations exceeding 10⁷ PFU/ml are significantly more efficacious in eradicating bacteria within a biofilm. The antibacterial phage activity decreases substantially by 3.54-fold when applied at 4 °C compared to temperatures above 25 °C. This analysis suggests that bacteriophages can be a promising solution for controlling biofilms in the food industry.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100170"},"PeriodicalIF":6.8,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000679/pdfft?md5=c368579415c79e3f41ca68cd79619c68&pid=1-s2.0-S2590207523000679-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis interaction with Candida albicans allows for aerobic escape, virulence and adherence","authors":"Caroline A. de Jongh ,&nbsp;Floris J. Bikker ,&nbsp;Teun J. de Vries ,&nbsp;Arie Werner ,&nbsp;Susan Gibbs ,&nbsp;Bastiaan P. Krom","doi":"10.1016/j.bioflm.2023.100172","DOIUrl":"10.1016/j.bioflm.2023.100172","url":null,"abstract":"<div><p>In the oral cavity <em>Candida albicans</em> interacts with many oral bacteria, including <em>Porphyromonas gingivalis</em>, both physically and metabolically. The aim of this <em>in vitro</em> study was to characterize these interactions and study their effects on the survival of <em>P. gingivalis</em>. First, metabolic interactions were evaluated by counting the colony forming units (CFU) after co-culturing. The results indicated that the anaerobic bacterium <em>P. gingivalis</em> survives under aerobic conditions when co-cultured with <em>C. albicans</em>. This is due to the oxygen consumption by <em>C. albicans</em> as determined by a reduction in survival upon the addition of Antimycin A. By measuring the protease activity, it was found that the presence of <em>C. albicans</em> induced gingipain activity by <em>P. gingivalis</em>, which is an important virulence factor. Adherence of <em>P. gingivalis</em> to hyphae of <em>C. albicans</em> was observed with a dynamic flow system. Using various <em>C. albicans</em> mutants, it was shown that the mechanism of adhesion was mediated by the cell wall adhesins, members of the agglutinin-like sequence (Als) family: Als3 and Als1. Furthermore, the two microorganisms could be co-cultured into forming a biofilm in which <em>P. gingivalis</em> can survive under aerobic culturing conditions, which was imaged using scanning electron microscopy. This study has further elucidated mechanisms of interaction, virulence acquisition and survival of <em>P. gingivalis</em> when co-cultured with <em>C. albicans</em>. Such survival could be essential for the pathogenicity of <em>P. gingivalis</em> in the oxygen-rich niches of the oral cavity. This study has emphasized the importance of interaction between different microbes in promoting survival, virulence and attachment of pathogens, which could be essential in facilitating penetration into the environment of the host.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100172"},"PeriodicalIF":6.8,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000692/pdfft?md5=cc574f1ced78a44065341f6675fc1e1b&pid=1-s2.0-S2590207523000692-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}