Biofilm最新文献

{"title":"Alteration of oral microbial biofilms by sweeteners","authors":"Geum-Jae Jeong ,&nbsp;Fazlurrahman Khan ,&nbsp;Nazia Tabassum ,&nbsp;Young-Mog Kim","doi":"10.1016/j.bioflm.2023.100171","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100171","url":null,"abstract":"<div><p>There is a growing interest in using sweeteners for taste improvement in the food and drink industry. Sweeteners were found to regulate the formation or dispersal of structural components of microbial biofilms. Dietary sugars may enhance biofilm formation and facilitate the development of antimicrobial resistance, which has become a major health issue worldwide. In contrast, bulk and non-nutritive sweeteners are also beneficial for managing microbial infections. This review discusses the clinical significance of oral biofilms formed upon the administration of nutritive and non-nutritive sweeteners. The underlying mechanism of action of sweeteners in the regulation of mono- or poly-microbial biofilm formation and destruction is comprehensively discussed. Bulk and non-nutritive sweeteners have also been used in conjunction with antimicrobial substances to reduce microbial biofilm formation. Formulations with bulk and non-nutritive sweeteners have been demonstrated to be particularly efficient in this regard. Finally, future perspectives with respect to advancing our understanding of mechanisms underlying biofilm regulation activities of sweeteners are presented as well. Several alternative strategies for the application of bulk sweeteners and non-nutritive sweeteners have been employed to control the biofilm-forming microbial pathogens. Gaining insight into the underlying mechanisms responsible for enhancing or inhibiting biofilm formation and virulence properties by both mono- and poly-microbial species in the presence of the sweetener is crucial for developing a therapeutic agent to manage microbial infections.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"7 ","pages":"Article 100171"},"PeriodicalIF":6.8,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000680/pdfft?md5=a95551568523227db9b616a91d58becb&pid=1-s2.0-S2590207523000680-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138839087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hands On Biofilm! A multidisciplinary public engagement event using kombucha tea pellicle as an accessible example of biofilm","authors":"Joanna Verran ,&nbsp;Jane Wood ,&nbsp;James Redfern ,&nbsp;Haleh Moravej ,&nbsp;Natascha Radclyffe-Thomas","doi":"10.1016/j.bioflm.2023.100169","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100169","url":null,"abstract":"<div><p>Public engagement with science has become increasingly important for the scientific community. There are many documented public engagement events that focus on aspects of microbiology, but relatively few utilise biofilms as a topic, despite their importance. Kombucha tea pellicles are easy to grow biofilms, facilitating their use within the public domain as examples of these complex communities.</p><p>The aim of this work was to deliver a public engagement event that introduced visitors to general concepts about biofilm, and applications around sustainability, using kombucha. The event encouraged visitors to: build a biofilm using model clay; inoculate kombucha tea cultures using different incubation conditions, as part of a citizen science experiment to assess impact on pellicle biofilm yield; create garments and drapes on mini-mannequins using dried kombucha pellicle fabric, and demonstrate the range and importance of fermented foods (including kombucha tea), and ‘good bacteria’. Quantitative and qualitative indicators of engagement were built into the activities.</p><p>More than 1200 visitors, mainly in family groups, visited the event over a 4-h period. Knowledge of biofilms was low at the beginning of the event. Participation in all activities was high. Indicators of quantitative engagement were impressive, but it was difficult to obtain qualitative evidence other than observations from the delivery team (nineteen members) because of the intensity of the event and volume of visitors.</p><p>The event was clearly successful in terms of fulfilment of aims, audience engagement and enthusiasm: the embedded evaluations helped to evidence the impact and reach of the event, enabling confidence in dissemination of good practice in the enhancement of public understanding of the importance of biofilm in general, and kombucha in particular.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100169"},"PeriodicalIF":6.8,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000667/pdfft?md5=3aaced47a72b44580bea5b59759e1659&pid=1-s2.0-S2590207523000667-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138549269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of nontuberculous mycobacteria biofilms induces a highly vulnerable to antibiotic killing phenotype","authors":"Nikola Kurbatfinski ,&nbsp;Preston J. Hill ,&nbsp;Noah Tobin ,&nbsp;Cameron N. Kramer ,&nbsp;Joseph Wickham ,&nbsp;Steven D. Goodman ,&nbsp;Luanne Hall-Stoodley ,&nbsp;Lauren O. Bakaletz","doi":"10.1016/j.bioflm.2023.100166","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100166","url":null,"abstract":"<div><h3>Objectives</h3><p>Structural or mucus hypersecretory pulmonary diseases such as cystic fibrosis (CF), wherein viscous mucus accumulates and clearance functions are impaired, predispose people to lung infection by inhaled bacteria that form biofilm aggregates. Nontuberculous mycobacteria (NTM), primarily <em>Mycobacterium abscessus</em> and <em>Mycobacterium avium,</em> are the growing cause of these lung infections and are extremely challenging to treat due to antibiotic recalcitrance. Better therapeutic approaches are urgently needed. We developed a humanized monoclonal antibody (HuTipMab) directed against a biofilm structural linchpin, the bacterial DNABII proteins, that rapidly disrupts biofilms and generates highly vulnerable <u>n</u>ewly <u>rel</u>eased bacteria (NRel).</p></div><div><h3>Methods</h3><p>HuTipMab's ability to recognize HupB, NTM's DNABII homologue was determined by ELISA. Relative ability of HuTipMab to disrupt biofilms formed by lab-passaged and clinical isolates of NTM was assessed by CLSM. Relative sensitivity of NTM NRel to antibiotic killing compared to when grown planktonically was evaluated by plate count.</p></div><div><h3>Results</h3><p>HuTipMab recognized HupB and significantly disrupted NTM biofilms in a time- and dose-dependent manner. Importantly, NTM NRel of lab-passaged and clinical isolates were now highly sensitive to killing by amikacin and azithromycin.</p></div><div><h3>Conclusions</h3><p>If successful, this combinatorial treatment strategy would empower existing antibiotics to more effectively kill NTM newly released from a biofilm by HuTipMab and thereby both improve clinical outcomes and perhaps decrease length of antibiotic treatment for people that are NTM culture-positive.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100166"},"PeriodicalIF":6.8,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000631/pdfft?md5=13ac21e7eb637e2c48f1de840b7e5cee&pid=1-s2.0-S2590207523000631-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138467457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A microtiter peg lid with ziggurat geometry for medium-throughput antibiotic testing and in situ imaging of biofilms","authors":"Sarah K. Childs ,&nbsp;A-Andrew D. Jones III","doi":"10.1016/j.bioflm.2023.100167","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100167","url":null,"abstract":"<div><p>Bacteria biofilm responses to disinfectants and antibiotics are quantified and observed using multiple methods, though microscopy, particularly confocal laser scanning microscopy (CLSM) is preferred due to speed, a reduction in user error, and <em>in situ</em> analysis. CLSM can resolve biological and spatial heterogeneity of biofilms in 3D with limited throughput. The microplate peg-lid-based assay, described in ASTM E2799-22, is a medium-throughput method for testing biofilms but does not permit <em>in situ</em> imaging. Breaking off the peg, as recommended by the manufacturer, risks sample damage, and is limited to easily accessible pegs. Here we report modifications to the peg optimized for <em>in situ</em> visualization and visualization of all pegs. We report similar antibiotic challenge recovery via colony formation following the ASTM E2799-22 protocol and <em>in situ</em> imaging. We report novel quantifiable effects of antibiotics on biofilm morphologies, specifically biofilm streamers. The new design bridges the MBEC® assays design that selects for biofilm phenotypes with <em>in situ</em> imaging needs.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100167"},"PeriodicalIF":6.8,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000643/pdfft?md5=9f1e8da418a8c5c3c2eff2b251252f80&pid=1-s2.0-S2590207523000643-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138423054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selected flavonoids exhibit antibiofilm and antibacterial effects against Vibrio by disrupting membrane integrity, virulence and metabolic activities","authors":"Olajide Sunday Faleye,&nbsp;Jin-Hyung Lee,&nbsp;Jintae Lee","doi":"10.1016/j.bioflm.2023.100165","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100165","url":null,"abstract":"<div><p><em>Vibrio parahaemolyticus</em> is a high-risk foodborne pathogen associated with raw or undercooked seafoods and its biofilm forming potential has become a threat to food safety and economic values. Hence, this study aims to examine the antibacterial and antibiofilm activities as well as virulence inhibitory effects of selected flavonoids against <em>V. parahaemolyticus</em>. Out of the sixteen flavonoid derivatives, 6-aminoflavone (6-AF), 3,2-dihydroxyflavone (3,2-DHF) and 2,2-dihydroxy-4-methoxybenzophenone (DHMB) were found as active biofilm inhibitors. 3,2-DHF and DHMB had minimum inhibitory concentrations of 20 and 50 μg/mL respectively against <em>Vibrio</em> planktonic cells and displayed superior antibacterial activities to standard controls. Also, they disrupted preformed biofilms and suppressed virulence properties including motilities, cell hydrophobicity and aggregation. They impaired iron acquisition mechanism and hemolysin production at sub-MICs as supported by transcriptomic studies. Interestingly, the flavonoids interfered with the metabolic activity, cell division and membrane permeability to exert antibiofilm and antibacterial activities. 6-AF and 3,2-DHF were non-toxic in the <em>C. elegans</em> model and showed excellent capacity to protect shrimps from biodeterioration. Furthermore, the flavonoids inhibited biofilm formation by <em>V. harveyi</em>, <em>Staphylococcus aureus</em> and <em>Salmonella typhimurium</em> and the mixed-species biofilm with <em>Vibrio</em>. This study discovered flavonoid derivatives, especially 3,2-DHF as potential bioactive compounds capable of offering protection from risks associated with biofilm formation by <em>V. parahaemolyticus</em> and other food pathogens.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100165"},"PeriodicalIF":6.8,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259020752300062X/pdfft?md5=54381abe1cbb1a465874ef2fceea356f&pid=1-s2.0-S259020752300062X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling host-pathogen interactions by biofilm infected human wound models","authors":"Jana Wächter ,&nbsp;Pia K. Vestweber ,&nbsp;Viktoria Planz,&nbsp;Maike Windbergs","doi":"10.1016/j.bioflm.2023.100164","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100164","url":null,"abstract":"<div><p>Approximately 80 % of persistent wound infections are affected by the presence of bacterial biofilms, resulting in a severe clinical challenge associated with prolonged healing periods, increased morbidity, and high healthcare costs. Unfortunately, <em>in vitro</em> models for wound infection research almost exclusively focus on early infection stages with planktonic bacteria. In this study, we present a new approach to emulate biofilm-infected human wounds by three-dimensional human <em>in vitro</em> systems. For this purpose, a matured biofilm consisting of the clinical key wound pathogen <em>Pseudomonas aeruginosa</em> was pre-cultivated on electrospun scaffolds allowing for non-destructive transfer of the matured biofilm to human <em>in vitro</em> wound models. We infected tissue-engineered human <em>in vitro</em> skin models as well as <em>ex vivo</em> human skin explants with the biofilm and analyzed structural tissue characteristics, biofilm growth behavior, and biofilm-tissue interactions. The structural development of biofilms in close proximity to the tissue, resulting in high bacterial burden and <em>in vivo</em>-like morphology, confirmed a manifest wound infection on all tested wound models, validating their applicability for general investigations of biofilm growth and structure. The extent of bacterial colonization of the wound bed, as well as the subsequent changes in molecular composition of skin tissue, were inherently linked to the characteristics of the underlying wound models including their viability and origin. Notably, the immune response observed in viable <em>ex vivo</em> and <em>in vitro</em> models was consistent with previous <em>in vivo</em> reports. While <em>ex vivo</em> models offered greater complexity and closer similarity to the <em>in vivo</em> conditions, <em>in vitro</em> models consistently demonstrated higher reproducibility. As a consequence, when focusing on direct biofilm-skin interactions, the viability of the wound models as well as their advantages and limitations should be aligned to the particular research question of future studies. Altogether, the novel model allows for a systematic investigation of host-pathogen interactions of bacterial biofilms and human wound tissue, also paving the way for development and predictive testing of novel therapeutics to combat biofilm-infected wounds.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100164"},"PeriodicalIF":6.8,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207523000618/pdfft?md5=9f51e1d132f50c453cff2f3eccb73f77&pid=1-s2.0-S2590207523000618-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candida causes recurrent vulvovaginal candidiasis by forming morphologically disparate biofilms on the human vaginal epithelium","authors":"Yihong Pan ,&nbsp;Yao Sun ,&nbsp;Lanqian Chen ,&nbsp;Yali Cheng ,&nbsp;Panpan Jin ,&nbsp;Weidan Zhang ,&nbsp;Lingzhi Zheng ,&nbsp;Junyan Liu ,&nbsp;Tieli Zhou ,&nbsp;Zhenbo Xu ,&nbsp;Cheng Li ,&nbsp;Xenia Kostoulias ,&nbsp;Cathy J. Watson ,&nbsp;David McGiffin ,&nbsp;Anton Y. Peleg ,&nbsp;Yue Qu","doi":"10.1016/j.bioflm.2023.100162","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100162","url":null,"abstract":"<div><h3>Background</h3><p>Recurrent vulvovaginal candidiasis (RVVC) is a recalcitrant medical condition that affects many women of reproductive age. The importance of biofilm formation by <em>Candida</em> in RVVC has been recently questioned. This study aimed to elucidate the fundamental growth modes of <em>Candida</em> in the vagina of patients with RVVC or sporadic vulvovaginal candidiasis (VVC) and to assess their roles in the persistence of RVVC.</p></div><div><h3>Methods</h3><p>Vaginal tissues were sampled from twelve patients clinically and microbiologically diagnosed as RVVC or VVC at a post-antifungal-treatment and asymptomatic period. High-resolution scanning electron microscopy, fluorescence in situ hybridization in combination with <em>Candida</em>-specific 18S rRNA probes and viable fungal burden were used to qualitatively and quantitatively evaluate <em>Candida</em> growth in the human vagina. The presence of <em>Candida</em> biofilm extracellular polymeric substances was examined using confocal laser scanning microscopy and biopsy sections pre-stained with Concanavalin A. Histopathological analysis was carried out on infected vaginal tissues stained with hematoxylin and eosin. Lastly, the susceptibility of epithelium-associated <em>Candida</em> biofilms to fluconazole at the peak serum concentration was evaluated.</p></div><div><h3>Results</h3><p><em>Candida</em> species grew on the vaginal epithelium of RVVC patients as morphologically disparate biofilms including monolayers, microcolonies, and macro-colonies, in addition to sporadic adherent cells. <em>Candida</em> biofilm growth on the vaginal epithelium was associated with mild lymphocytic infiltration of the vaginal mucosa. These epithelium-based <em>Candida</em> biofilms presented an important characteristic contributing to the persistence of RVVC that is the high tolerance to fluconazole.</p></div><div><h3>Conclusions</h3><p>In summary, our study provides direct evidence to support the presence of <em>Candida</em> biofilms in RVVC and an important role of biofilm formation in disease persistence.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100162"},"PeriodicalIF":6.8,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259020752300059X/pdfft?md5=76948a01969b74a6adf6661c7a3940da&pid=1-s2.0-S259020752300059X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of quantification methods for an endoscope lumen biofilm model","authors":"Bruno Haas ,&nbsp;Sarah James ,&nbsp;Albert E. Parker ,&nbsp;Marie-Claude Gagnon ,&nbsp;Noémie Goulet ,&nbsp;Philippe Labrie","doi":"10.1016/j.bioflm.2023.100163","DOIUrl":"10.1016/j.bioflm.2023.100163","url":null,"abstract":"<div><p>Biofilm has been implicated in multi-drug resistant organism outbreaks following endoscopic procedures. Automated Endoscope Reprocessors (AER) are devices validated to clean and disinfect endoscopes per applicable standards. The ISO 15883 part 4 standard guides performance testing validation of AERs, including cleaning performance using a biofilm test soil. The standard recommends assessment of biofilm reduction using protein or carbohydrate quantification methods. The aim of this study was to assess the suitability of various quantification methods using the ISO biofilm model.</p><p>The ISO 15883 part 5 biofilm test soil method was used to grow biofilm within lumens representative of endoscopes channels. The biofilm was then quantified using five methods: Crystal Violet (CV), Colony Forming Units (CFU), Total Organic Carbon (TOC), protein assay with Orthophtalaldehyde (OPA), and protein assay by micro bicinchoninic acid (μBCA). The five methods were statistically analyzed for their ability to assess biofilm reduction on samples accurately and precisely. In addition, the quantification methods were compared to demonstrate statistical equivalency, and thus their suitability for assessing biofilm cleaning performance testing of AERs.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100163"},"PeriodicalIF":6.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71523487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of anode material and dispersal limitation on the performance and biofilm community in microbial electrolysis cells","authors":"Marie Abadikhah ,&nbsp;Ming Liu ,&nbsp;Frank Persson ,&nbsp;Britt-Marie Wilén ,&nbsp;Anne Farewell ,&nbsp;Jie Sun ,&nbsp;Oskar Modin","doi":"10.1016/j.bioflm.2023.100161","DOIUrl":"10.1016/j.bioflm.2023.100161","url":null,"abstract":"<div><p>In a microbial electrolysis cell (MEC), the oxidization of organic compounds is facilitated by an electrogenic biofilm on the anode surface. The biofilm community composition determines the function of the system. Both deterministic and stochastic factors affect the community, but the relative importance of different factors is poorly understood. Anode material is a deterministic factor as materials with different properties may select for different microorganisms. Ecological drift is a stochastic factor, which is amplified by dispersal limitation between communities. Here, we compared the effects of three anode materials (graphene, carbon cloth, and nickel) with the effect of dispersal limitation on the function and biofilm community assembly. Twelve MECs were operated for 56 days in four hydraulically connected loops and shotgun metagenomic sequencing was used to analyse the microbial community composition on the anode surfaces at the end of the experiment. The anode material was the most important factor affecting the performance of the MECs, explaining 54–80 % of the variance observed in peak current density, total electric charge generation, and start-up lag time, while dispersal limitation explained 10–16 % of the variance. Carbon cloth anodes had the highest current generation and shortest lag time. However, dispersal limitation was the most important factor affecting microbial community structure, explaining 61–98 % of the variance in community diversity, evenness, and the relative abundance of the most abundant taxa, while anode material explained 0–20 % of the variance. The biofilms contained nine <em>Desulfobacterota</em> metagenome-assembled genomes (MAGs), which made up 64–89 % of the communities and were likely responsible for electricity generation in the MECs. Different MAGs dominated in different MECs. Particularly two different genotypes related to <em>Geobacter benzoatilyticus</em> competed for dominance on the anodes and reached relative abundances up to 83 %. The winning genotype was the same in all MECs that were hydraulically connected irrespective of anode material used.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100161"},"PeriodicalIF":6.8,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/c9/main.PMC10582064.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-microbe interactions in chronic rhinosinusitis biofilms and models for investigation","authors":"Emily J. Vanderpool ,&nbsp;Kendra P. Rumbaugh","doi":"10.1016/j.bioflm.2023.100160","DOIUrl":"https://doi.org/10.1016/j.bioflm.2023.100160","url":null,"abstract":"<div><p>Chronic rhinosinusitis (CRS) is a debilitating condition characterized by long-lasting inflammation of the paranasal sinuses. It affects a significant portion of the population, causing a considerable burden on individuals and healthcare systems. The pathogenesis of CRS is multifactorial, with bacterial infections playing a crucial role in CRS development and persistence. In recent years, the presence of biofilms has emerged as a key contributor to the chronicity of sinusitis, further complicating treatment and exacerbating symptoms. This review aims to explore the role of biofilms in CRS, focusing on the involvement of the bacterial species <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>, their interactions in chronic infections, and model systems for studying biofilms in CRS. These species serve as an example of how microbial interplay can influence disease progression and exemplify the need for continued investigation and innovation in CRS research.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100160"},"PeriodicalIF":6.8,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50171105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}