Brazilian Journal of Biology最新文献_第8页

Sanitary and physiological quality of Delonix regia seeds inoculated with Trichoderma asperellum and Saccharomyces cerevisiae. 曲霉木霉和酿酒酵母接种龙葵种子的卫生品质和生理品质。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.297829

I N B M Moura, J N Silva, K D S V Messias, M A C Lima, G L F Souza, J M F L Cruz, J B Lira, O R Farias, G V G Pádua, M H B S Rodrigues, A G Souza, E U Alves, L C Nascimento

{"title":"Sanitary and physiological quality of Delonix regia seeds inoculated with Trichoderma asperellum and Saccharomyces cerevisiae.","authors":"I N B M Moura, J N Silva, K D S V Messias, M A C Lima, G L F Souza, J M F L Cruz, J B Lira, O R Farias, G V G Pádua, M H B S Rodrigues, A G Souza, E U Alves, L C Nascimento","doi":"10.1590/1519-6984.297829","DOIUrl":"10.1590/1519-6984.297829","url":null,"abstract":"Delonix regia is an ornamental tree species that is widely used in Brazilian landscaping. It is propagated by seeds, the quality of which can be compromised by the presence of pathogenic fungi. The use of biological agents, such as Trichoderma asperellum and Saccharomyces cerevisiae, in seed treatment has proven to be a promising alternative to the use of synthetic fungicides, promoting phytosanitary and physiological benefits to seeds. Therefore, the objective of this work was to determine the antagonistic effects of the agents T. asperellum and S. cerevisiae on the sanitary and physiological quality of D. regia seeds. The experimental design used was completely randomized, with treatments distributed in a 2 × 5 + 1 factorial scheme, with two biocontrol agents (T. asperellum and S. cerevisiae), five concentrations (0.0; 0.5; 1.0; 1.5 and 2.0 mL) + one additional treatment (seeds treated with fungicide). Seed health, germination, emergence and initial seedling development were evaluated. The fungi associated with the seeds were from the genera Fusarium sp., Cladosporium sp., Chaetomium sp., Aspergillus sp., Lasiodiplodia sp. and Rhizoctonia sp. The use of T. asperellum is effective in controlling Fusarium sp. in D. regia seeds, although its use may compromise physiological aspects of the seeds, such as germination and vigor. On the other hand, the application of S. cerevisiae was promising for reducing the incidence of microorganisms and, simultaneously, promoting improvements in the physiological quality of seeds. These findings indicate the potential of using biological agents, especially S. cerevisiae, as a sustainable strategy for the treatment of forest seeds.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e297829"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly cleanup: the power of nano-bioremediation in microplastic management. 生态友好型清洁：纳米生物修复在微塑料管理中的作用。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.302241

I Hammami, H Almahasheer, N Alahmady, D S Al-Qahtani, S H Alharoon, R S Alghamdi, N M Al-Qahtani, S M Moqbel, N I Al-Dabbous

{"title":"Eco-friendly cleanup: the power of nano-bioremediation in microplastic management.","authors":"I Hammami, H Almahasheer, N Alahmady, D S Al-Qahtani, S H Alharoon, R S Alghamdi, N M Al-Qahtani, S M Moqbel, N I Al-Dabbous","doi":"10.1590/1519-6984.302241","DOIUrl":"10.1590/1519-6984.302241","url":null,"abstract":"Microplastic (MP) pollution has emerged as a defining environmental challenge of the 21st century, threatening ecosystems, food security, and human health at an unprecedented scale. Conventional remediation methods-such as filtration, coagulation, and advanced oxidation-remain inefficient, energy-intensive, and incapable of addressing nanoscale fragments or preventing secondary contamination. This review provides a comprehensive and forward-looking synthesis ofnanoparticle-assisted bioremediation, an emerging paradigm that integrates microbial enzymatic degradation with engineered and green-synthesized nanomaterials to achieve sustainable plastic depolymerization. We critically examine how magnetic nanoparticles enable rapid adsorption and recovery, how photocatalytic metal oxides (TiO2, ZnO, Fe2O3, CeO2) and plasmonic hybrids generate reactive oxygen species to oxidatively cleave polymer chains, and how nano-bio hybrid systems stabilize enzymes, enhance substrate accessibility, and accelerate mineralization into CO2, H2O, and biomass. Special emphasis is placed on green-synthesized nanoparticles derived from plants and microbes, which offer enhanced environmental compatibility and catalytic efficiency. The review also evaluates the mechanistic underpinnings, kinetic parameters, and techno-economic feasibility of these systems, identifying critical challenges related to nanoparticle aggregation, enzyme instability, and ecological safety. By uniting insights from nanotechnology, microbiology, and environmental engineering, this work delineates a strategic roadmap toward biodegradable, multifunctional nanomaterials and biofilm-enhanced hybrid reactors capable of large-scale deployment. Nano-bioremediation represents not merely an incremental advance but a transformative step toward a circular, low-carbon bioeconomy, offering a realistic and scalable pathway to mitigate global microplastic pollution and restore environmental integrity.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e302241"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and toxicological evaluation of (E)-2-((3,7-dimethylocta-2,6-dien-1-yl)oxy)ethyl acetate. (E)-2-（（3,7-二甲基-2,6-二烯-1-基）氧）乙酸乙酯的抗菌及毒理学评价

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.302367

R O Nóbrega, G N Alves, D A Santos, A M A Moura, N A Melo, L A Ramalho, J G F M Galvão, F S V Lins, C I S Medeiros, J C R Freitas, E O Lima, A A Oliveira Filho

{"title":"Antibacterial and toxicological evaluation of (E)-2-((3,7-dimethylocta-2,6-dien-1-yl)oxy)ethyl acetate.","authors":"R O Nóbrega, G N Alves, D A Santos, A M A Moura, N A Melo, L A Ramalho, J G F M Galvão, F S V Lins, C I S Medeiros, J C R Freitas, E O Lima, A A Oliveira Filho","doi":"10.1590/1519-6984.302367","DOIUrl":"10.1590/1519-6984.302367","url":null,"abstract":"Klebsiella pneumoniae is a Gram-negative bacterium of great clinical relevance, responsible for severe infections and frequently associated with multidrug resistance. In this context, the synthesis of derivatives from natural products emerges as a promising alternative for the development of new compounds with biological and pharmacological potential. In the search for new compounds, the present study aimed to evaluate the antibacterial activity of (E)-2-((3,7-dimethylocta-2,6-dien-1-yl)oxy)ethyl acetate against K. pneumoniae strains. To achieve this goal, in silico methodologies were employed using the software PASS online®, Molinspiration®, admetSAR®, and molecular docking. For the in vitro studies, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using 96-well plates, along with combination tests with conventional synthetic antibacterials through disk diffusion. The main results of this study indicated that TSM-17 exhibited low theoretical toxicity. Through molecular docking, it was possible to observe the interaction of TSM-17 with the enzymatic active site of topoisomerase IV. The MIC of TSM-17 ranged from 250 µg/mL to 500 µg/mL against K. pneumoniae strains, indicating good antimicrobial activity, and it also showed bactericidal activity against most strains. Furthermore, the combination of TSM-17 with conventional antimicrobials, especially tetracycline, gentamicin, and cephalothin, resulted in synergistic effects, enhancing its efficacy. Thus, this study highlights the potential of the TSM-17 compound as a promising candidate for the development of new antibacterial agents against K. pneumoniae and reinforces the relevance of natural product-derived compounds in the search for new therapeutic alternatives to combat antimicrobial resistance.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e302367"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of soybean genotypes resistant to stink bug complex and key agronomic traits for selection. 大豆抗臭虫复合体基因型鉴定及关键农艺性状选择。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.302518

E L Grolli, J S Souza, C V G Azevedo, B H P Val, F L C Mingotte, J B Pinheiro, S H Unêda-Trevisoli

{"title":"Identification of soybean genotypes resistant to stink bug complex and key agronomic traits for selection.","authors":"E L Grolli, J S Souza, C V G Azevedo, B H P Val, F L C Mingotte, J B Pinheiro, S H Unêda-Trevisoli","doi":"10.1590/1519-6984.302518","DOIUrl":"10.1590/1519-6984.302518","url":null,"abstract":"The stink bug complex is characterized as an important soybean pest that demands the development of cultivars resistant to the attack of these insects to reduce losses in soybean quality and productivity and, therefore, develop a more sustainable agriculture. This study aimed at selecting soybean genotypes with good agronomic traits, including resistance to stink bug complex and identify the key traits that assist in this selection. 256 genotypes obtained by crossing IAC-100 (resistant) × CD-215 (susceptible) were evaluated under natural field infestation, in the 2014/15 harvest using the16x16alpha-lattice design. The multivariate statistical analysis of principal components and non-hierarchical K-means clustering method tested 11 agronomic traits of interest. The genotypes selected for resistance to stink bugs complex were IAC 100, 2, 18, 22, 25, 38, 48, 69, 71, 73, 110, 121, 132, 133, 149, 150, 171, 178, 181, 202, 214, 217, 219, 241 and 242. The studied traits as yield, good seed weight, leaf retention, and 100 seed weight can be used to select resistant genotypes.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e302518"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspects of the biology of Mugil curema (Osteichthyes, Mugilidae) in the Amazon coast, Brazil. 巴西亚马逊河沿岸木鱼（木鱼科）的生物学特征。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.299258

M B Figueiredo, M L F Melo, C A F Fernandes, L N da Silva

{"title":"Aspects of the biology of Mugil curema (Osteichthyes, Mugilidae) in the Amazon coast, Brazil.","authors":"M B Figueiredo, M L F Melo, C A F Fernandes, L N da Silva","doi":"10.1590/1519-6984.299258","DOIUrl":"10.1590/1519-6984.299258","url":null,"abstract":"Mugil curema is an estuarine and coastal fish species widely distributed in tropical and subtropical regions. It is an important resource for fisheries in Maranhão, among the most exploited and commercialized species. Given its relevance, studies on its reproductive dynamics are essential for effective stock management. This study aimed to analyze the reproductive aspects of M. curema in an estuarine area of the Northeast region of Brazil. Specimens were collected monthly from January to December 2021 in the estuary of the Paciência River, Maranhão, and morphometric data were analyzed to determine maturation stages, reproductive indices, and the size at first maturation. A total of 942 samples of M. curema were analyzed (827 female and 115 male). Females were more abundant (7.19F:1M), with a significantly larger mean size (t-test, p<0.05). The mean total length and mean total weight were 20.44 (± 3.38 cm) and 82.29 g (± 32.20 g), respectively. The weight-length relationship revealed distinct growth patterns: females exhibited positive allometry, whereas males exhibited negative allometry. A higher proportion of juveniles was recorded, indicating that the area functions as a nursery ground. Based on the gonadosomatic index (GSI), peak reproductive activity occurred in May and July; however, females with hydrated oocytes were also observed in other months. A correlation was observed between precipitation and reproductive rates. Histological analysis indicated total spawning, with synchronous development of two oocyte groups. The estimated length at first sexual maturity for both sexes was 19.04 cm. The reproductive aspects of M. curema appear closely linked to environmental conditions and seasonal cycles, highlighting the need for targeted management strategies to ensure the sustainability and conservation of this key fishery resource.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e299258"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico analysis of gene family and ribosome-inactivating proteins (RIPs) from Jatropha curcas L. 麻疯树基因家族及核糖体失活蛋白的计算机分析。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.304102

F C Oliveira, S A Silva, J A Castro, R J S Silva, A G Prazeres, I B S Jesus, H Y G Albuquerque, C S S Filadelfo, D F M Cortes, R M F Silva, R S Santos, H C P Moura, R F C Moreira, C R Filadelfo

{"title":"In silico analysis of gene family and ribosome-inactivating proteins (RIPs) from Jatropha curcas L.","authors":"F C Oliveira, S A Silva, J A Castro, R J S Silva, A G Prazeres, I B S Jesus, H Y G Albuquerque, C S S Filadelfo, D F M Cortes, R M F Silva, R S Santos, H C P Moura, R F C Moreira, C R Filadelfo","doi":"10.1590/1519-6984.304102","DOIUrl":"10.1590/1519-6984.304102","url":null,"abstract":"Ribossome-inactivating proteins (RIPs) are found in several plant, fungal, and bacterial species and play an important role in the plant defense system against phytopathogens. The present study aimed to characterize, in silico, the RIP gene and protein family in Jatropha curcas L. Genome annotation in the Jatropha curcas Database (JCDB) enabled the identification of a total of twelve RIP genes. The genes JcRIP1 to JcRIP11 encode curcin, a type I RIP found in physic nut, whereas JcRIP12 is a gene similar to those encoding ricin, a type II RIP present in Ricinus communis L. Light-responsive, MYB, and MYC cis-elements were identified in all promoter regions of the JcRIP genes. To a lesser extent, cis-elements responsive to biotic and abiotic stresses and phytohormones were also detected. JcRIP3 showed the highest expression among the analyzed tissues. In addition to seeds and leaves, RIP gene expression was also observed in stems, roots, and flowers. The number of amino acid residues of physic nut RIP proteins ranged from 291 (JcRIP8) to 551 (JcRIP12). Eight of the twelve JcRIPs exhibited a well-conserved EAAR motif (Glu185-Ala186-Ala187-Arg188). The YFF motif (Tyr99-Phe100-Phe101) was conserved in all JcRIPs analyzed in this study. The results of this work provide knowledge about conditions and locations of JcRIP gene expression, as well as the characteristics of corresponding proteins. This work can contribute to future genetic improvement studies focused on biotic stress, molecular docking analyses aimed at reducing/inhibiting harmfulness, and in vitro tests against phytopathogens.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e304102"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity and control of infections caused by motile Aeromonas in freshwater fish: immunostimulation strategies. 淡水鱼运动气单胞菌感染的致病性和控制：免疫刺激策略。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.296959

P R Santos, J P F Rufino, B A S Cavero, F D F Farias, A C N Alves, K R S Barbosa, P H R Aride, A T Oliveira

{"title":"Pathogenicity and control of infections caused by motile Aeromonas in freshwater fish: immunostimulation strategies.","authors":"P R Santos, J P F Rufino, B A S Cavero, F D F Farias, A C N Alves, K R S Barbosa, P H R Aride, A T Oliveira","doi":"10.1590/1519-6984.296959","DOIUrl":"10.1590/1519-6984.296959","url":null,"abstract":"In Brazil, despite favorable edaphoclimatic and hydrobiological conditions, the consolidation of the aquaculture sector is constrained by significant sanitary challenges, particularly bacterial infections caused by Aeromonas spp., which adversely affect fish farming, especially native species. In response, the widespread and often indiscriminate use of antimicrobials has contributed to the emergence and spread of multidrug-resistant strains. This study presents a narrative review addressing the clinical aspects, sanitary challenges associated with Aeromonas infections, and prophylactic strategies, with an emphasis on immunostimulation in freshwater fish, focusing on products derived from Saccharomyces cerevisiae, such as nucleotides and mannan-oligosaccharides. Intrinsic and extrinsic factors, including the cultivated species, ontogenetic stage, environmental conditions, and supplementation protocols, modulate the immunophysiological responses to these bioactive compounds and require specific scientific validation for each species. Therefore, there is an urgent need to advance research focused on Neotropical ichthyofauna, with particular emphasis on economically critical native species such as Colossoma macropomum, Arapaima gigas, Brycon amazonicus, Piaractus mesopotamicus, Rhamdia quelen, among others.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e296959"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147790125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How do plant growth-promoting bacteria and auxin influence the nutrition and morphophysiology of soybean? 植物促生菌和生长素如何影响大豆的营养和形态生理？

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.299482

J P de C Souza, L G R Macedo, C C Santos, D Pereira, R A L Janse, A S Dias, S P Q Scalon

{"title":"How do plant growth-promoting bacteria and auxin influence the nutrition and morphophysiology of soybean?","authors":"J P de C Souza, L G R Macedo, C C Santos, D Pereira, R A L Janse, A S Dias, S P Q Scalon","doi":"10.1590/1519-6984.299482","DOIUrl":"10.1590/1519-6984.299482","url":null,"abstract":"Co-inoculation of plant growth-promoting bacteria and the application of hormones can be practical for soybeans. We aimed to evaluate the effect of co-inoculation methods of Azospirillum brasilense + Pseudomonas fluorescens and auxin application on the nutrition and morphophysiology of soybeans. The co-inoculation methods were: control; inoculation with Bradyrhizobium spp. only; and co-inoculation with Bradyrhizobium spp. and a commercial source containing A. brasilense + P. fluorescens applied via seed, via foliar at stage V3, or via seed + foliar, associated or not with the application of auxin at stage V4. The application of auxin had little influence on photochemical yield. The best values of photochemical activity in the reaction centers of photosystem II occurred in plants co-inoculated via seed + foliar. Photosynthesis was higher in plants co-inoculated via seed and via seed + foliar, as were intrinsic carboxylation and water use efficiency. The highest nitrogen contents were observed in the control treatment and in the seed + foliar co-inoculation, regardless of auxin application. The control plants showed higher potassium, magnesium, and sulfur contents, while auxin application increased calcium content in plants. The application of auxin contributed to an increase in leaf area and biomass of leaves, roots, and nodules. Co-inoculation via foliar and seed + foliar routes anticipated flowering and increased the number of pods per plant. Co-inoculation of A. brasilense + P. fluorescens via seed + foliar routes promoted improvements in soybean morphophysiology, while auxin application contributed positively to soybean morphophysiology and nutrition.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e299482"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147790028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary study on genetic diversity and phylogenetic relationships of Teropong Temanggung sheep based on mitochondrial cytochrome b sequences. 基于线粒体细胞色素b序列的Teropong Temanggung绵羊遗传多样性及系统发育关系的初步研究。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.301893

M R Ridlo, F Ariyanti, A G As Syauqi, A P Z N L Sari, A Haryanto, N Assihhah, Panjono, D Ermawati, B A Atmoko, A Ibrahim

{"title":"Preliminary study on genetic diversity and phylogenetic relationships of Teropong Temanggung sheep based on mitochondrial cytochrome b sequences.","authors":"M R Ridlo, F Ariyanti, A G As Syauqi, A P Z N L Sari, A Haryanto, N Assihhah, Panjono, D Ermawati, B A Atmoko, A Ibrahim","doi":"10.1590/1519-6984.301893","DOIUrl":"10.1590/1519-6984.301893","url":null,"abstract":"The Teropong Temanggung sheep, an indigenous Indonesian breed from Central Java, represents an important genetic resource for smallholder farmers. However, molecular genetic information on this population remains limited. To clarify its maternal genetic background, this preliminary study analyzed the mitochondrial cytochrome b (Cyt b) gene sequence. Blood samples from six (n = 6) Teropong Temanggung sheep were collected, DNA was extracted, and a complete Cyt b gene fragment was amplified and sequenced. Three single-nucleotide polymorphism sites produced two haplotypes. Analysis using MEGA software showed low haplotype diversity (Hd = 0.3333) and nucleotide diversity (Pi = 0.00088). Phylogenetic reconstruction revealed that five samples clustered within haplogroup B and one within haplogroup A, suggesting a mixed maternal lineage origin. These findings provide preliminary baseline information on maternal genetic variation in Teropong Temanggung sheep; however, given the limited sample size and the use of a single mitochondrial marker, they should not be interpreted as evidence of genome-wide genetic distinctiveness. The results serve as a foundation for future studies incorporating larger sample sizes and nuclear genetic markers to better characterize this local sheep population.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e301893"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine amnion membrane-hydroxyapatite composite enhances alveolar bone regeneration by modulating osteoclast activity and woven bone formation. 牛羊膜-羟基磷灰石复合材料通过调节破骨细胞活性和编织骨形成促进牙槽骨再生。

Brazilian Journal of Biology Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1590/1519-6984.296925

E Munadziroh, A Yuliati, T H Agustantina, R K Maharani, A Halizah, Y A Azzaim, M D C Surboyo, N Mohd

{"title":"Bovine amnion membrane-hydroxyapatite composite enhances alveolar bone regeneration by modulating osteoclast activity and woven bone formation.","authors":"E Munadziroh, A Yuliati, T H Agustantina, R K Maharani, A Halizah, Y A Azzaim, M D C Surboyo, N Mohd","doi":"10.1590/1519-6984.296925","DOIUrl":"10.1590/1519-6984.296925","url":null,"abstract":"Tooth extraction without proper socket prevention will lead to bone loss. Applying bovine amnion membrane-hydroxyapatite (BAM-HA composite) can maintain bone structure through osteoclast and woven bone formation. The purpose of the study to evaluate the different compositions of BAM-HA composite as socket preservation by analyzing osteoclast and woven bone formation. Thirty male Sprague-Dawley rats underwent lower anterior teeth extraction. Each socket treated by BAM-HA composite with different ratio or composition 30:70, 35:65, 40:60 and control group treated with BioOss collagen. Fourteen and twent-eight-days after treatment, the mandible was collected for histological analysis for osteoclast and woven bone formation using hematoxyline-eosine staining. Statistical analysis was performed using two-way ANOVA and Tukey's post-hoc test with p<0.05 consider as statistical differences. BAM-HA composite treated groups showed a reduction in osteoclast numbers compared to the control and BioOss groups (p<0.05), indicating a suppression of excessive bone resorption. Concurrently, BAM-HA composite-treated groups, particularly composition 30:70 and 35:65, exhibited the highest levels of woven bone formation (p<0.05), suggesting enhanced bone regeneration. The application of the BAM-HA composite enhances alveolar bone regeneration in Sprague-Dawley rats tooth extraction sockets by reducing osteoclast number and promoting woven bone formation, suggesting its potential as an effective bone graft material to maintain the alveolar bone height.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e296925"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0