Brazilian Journal of Biology最新文献

{"title":"Preparation technology of encapsulated homogenate of drone brood in the Republic of Kazakhstan.","authors":"K Zhumanov, R Shimelkova, B Seisenov, K Tajiyev, F A Chaudhary, I Demidova","doi":"10.1590/1519-6984.293764","DOIUrl":"10.1590/1519-6984.293764","url":null,"abstract":"<p><p>Drone brood homogenate is a little-studied bee product used in medicine, veterinary medicine and cosmetology for treatment and prophylaxis. It is a very nutritious substance with a high content of active components: proteins, lipids, fatty acids, carbohydrates, vitamins (A, B, E and D) and minerals. The aim of this study was to obtain homogenate of drone larvae by different methods and its encapsulation. The results of the study of encapsulated drone homogenate as a biologically active supplement are presented. The technology of encapsulation of homogenate of drone brood has been developed. The mixture with the homogenate of drone brood, polymer and modified corn starch is successfully homogenized, and calcium chloride is used to harden the capsules. The optimum composition for encapsulation of drone brood homogenate was selected. The physicochemical, mineral, amino acid and vitamin composition of the product was analyzed, confirming its high nutritional value. It was found that the homogenate is rich in carbohydrates (82.13%), proteins (2.4%), calcium (434.2 mg/100 g), magnesium (222.7 mg/100 g) and B vitamins. The encapsulation method with using sodium alginate allowed to ensure the stability and long storage of the product. It was found that bee families with laying queens older than two years are the most productive for obtaining drone brood. The results confirm the effectiveness of encapsulated homogenate as a biological active additive increase productivity and reproductive function of animals.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293764"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of enteroparasitosis in users of the clinical analysis laboratory of a municipality of the agreste region of Alagoas.","authors":"W S Passos Neto, L C A Novais, L R L Siqueira, M N Machado, A A M Mousinho, A L N Marques, T L G Q Maranhão, H B G Messias, R C F Lemos, J A Farias, M G S Cavalcanti, T J M Rocha","doi":"10.1590/1519-6984.290948","DOIUrl":"https://doi.org/10.1590/1519-6984.290948","url":null,"abstract":"<p><p>Parasitic diseases represent a significant challenge in the field of global public health, being closely linked to precarious sociodemographic and environmental conditions, which are more common in tropical and subtropical countries. This study aimed to describe the epidemiological profile of enteroparasitosis in the population attended by a private clinical analysis laboratory in the Agreste region of Alagoas. A retrospective cross-sectional descriptive study was conducted in a clinical analysis laboratory located in the city of Arapiraca-AL, using the results of fecal examinations from 01/01/2023 to 12/31/2023, among individuals aged 1 to 99 years, totaling 5,871 analyzed tests. The Hoffman, Pons, and Janer or Lutz Method, also known as the spontaneous sedimentation method, was used for the examination. As a result, 7% (431) of cases were positive for at least one intestinal parasite, with the most prevalent being the protozoa Entamoeba coli 33.26% (152), Giardia lamblia 16.47% (71), Entamoeba histolytica/Entamoeba dispar 11.60% (50), and Endolimax nana 0.93% (4). Regarding helminth infections, the most prevalent was Ascaris lumbricoides at 24.12% (104), followed by Enterobius vermicularis 3.71% (16), Hymenolepis nana 1.85% (8), Schistosoma mansoni 1.85% (8), Trichuris trichiura 0.92% (4), and Ancylostomatidae 0.92% (4). Thus, due to the significant portion of the population infected, the importance of implementing public policies aimed at improving sanitary education is evident.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e290948"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in green extraction technologies for pectin enhancing efficiency, sustainability, and functional properties: a systematic review.","authors":"R J Malpartida Yapias, F Ore Areche, J P Echevarria Victorio, J Paucarchuco Soto, G R Lobato Calderon, E R Palomino Santos, J M Montalvo Otivo, C Flores-Miranda, A Ruiz Rodriguez","doi":"10.1590/1519-6984.287792","DOIUrl":"10.1590/1519-6984.287792","url":null,"abstract":"<p><p>Plants produce pectin as a versatile biopolymer stemming from their cell walls where it shows importance in food together with pharmaceuticals and cosmetics because of its gel-forming ability and thickening and stabilizing traits. The extraction techniques for pectin traditionally demand high amounts of power alongside hazardous solvents while needing extended processing durations. The development of emerging green extraction technologies (ET) provides sustainable and efficient extraction methodologies. This analysis conducts a systematic assessment of different ETs for pectin extraction by examining UAE alongside MAE and includes evaluation of supercritical CO2, HPP, and PEF extraction methods. These methods present advantages such as higher extraction performance with lowered energy usage as well as better pectin rheological properties that enhance both gel strength and viscosity. ETs effectively maintain or magnify functional properties in pectin which increases its potential for high-end uses in food items and supplements and cosmetics. These technologies provide an environmentally beneficial method through their reduced reliance on solvents and reduced creation of waste products. Research alongside technological progress hopes to solve existing hurdles associated with equipment costs and scalability because these hurdles currently prevent widespread adoption of these methods, but the solutions will enable industrial-scale use. There exists an opportunity through green extraction technologies to create a sustainable future for industrial pectin production.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e287792"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Film coating of Enterolobium schomburgkii (Benth.) Benth seeds.","authors":"M M R Borges, C F Lisboa, D G Araújo, I R Teixeira, A M Antunes, H S D Barros, P H C Castro, C S Silva, C R A Dias","doi":"10.1590/1519-6984.292835","DOIUrl":"https://doi.org/10.1590/1519-6984.292835","url":null,"abstract":"<p><p>The growing demand for native seeds in forest restoration projects highlights the importance of technical and technological knowledge in the propagation and maintenance of forest species. Seed coating can facilitate direct seeding of these species. However, although the polymer polyvinyl alcohol (PVA) and the plasticizer glycerol have been studied for coating agricultural seeds, there is a lack of information about their behavior in forest species. Therefore, the objective of this research is to evaluate the applicability of film coating using polyvinyl alcohol and glycerol on forest seeds, specifically Enterolobium schomburgkii, and evaluate its influence on germination. The first experiment determined a suitable formulation for coating, for which three concentrations of PVA (1%, 3% and 5%) and glycerol (10%, 20% and 30%) were evaluated. To evaluate the effect of coatings on germination, a control was included, totaling 40 plots. Thickness, water vapor permeability, solubility and germination tests were carried out. The combination of 5% PVA and 10% glycerol was appropriate for coating E. schomburgkii seeds due to its properties and lack of deleterious effect on germination.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292835"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and partial characterization of a new fibrinolytic protease from salmon oyster mushroom from Amazonia.","authors":"E E P Barbosa, L Pimenta, K S Araújo, A K P Brito, S C P Batista, S R Martim, W R Gomes, M F S Teixeira","doi":"10.1590/1519-6984.289933","DOIUrl":"https://doi.org/10.1590/1519-6984.289933","url":null,"abstract":"<p><p>Edible mushrooms are excellent sources of enzymes, especially fibrinolytic proteases, which work to dissolve blood clots and can be obtained through different fermentative processes. This research evaluated the production of fibrinolytic protease from a specie of edible mushroom in different formulations of liquid cultures. Pleurotus ostreatoroseus was provided by the DPUA culture collection at the Federal University of Amazonas and cultivated on PDA agar supplemented with 0.5% (w/v) yeast extract. Liquid fermentation was carried out in an Erlenmeyer flask in 50 mL of GYP, MGYP, or Malt medium under stirring at 150 rpm at 30 °C, eight days. The extracts were recovered and dialyzed, and the liquid culture medium was selected based on the activity of fibrinolytic enzymes. In the recovered extracts, qualitative activity (fibrin plate) and quantitative activity of fibrinolytic proteases were determined, and the effect of pH, temperature, stability, ions, and inhibitors on enzymatic activity was evaluated. Pleurotus ostreatoroseus excreted proteases in all culture media tested. However, the translucent halo (12.59 ± 0.7 mm) and significant activity of fibrinolytic enzymes (449.32 ± 0.01 U/mL) was determined in GYP. In the dialyzed extract, P. ostreatoroseus had an increase in the excretion of fibrinolytic proteases (1,361.73 ± 0.09). Results indicated that in GYP extracts, proteases showed optimal activity at pH 8.0 and at 30 °C of the serine and metallo protease types. Thus, these biocatalysts have strong potential for use in the pharmaceutical, detergent sectors and food industries.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e289933"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryos extract from germinated yellow soybean (Glycine max [L.] Merrill) inhibits promastigotes form of Leishmania amazonensis.","authors":"P M Pedrote, D M D Bouts, C L A Passos, J D Inácio, L F O Gervazoni, R Zingali, M S Almeida, K M S Cabral, C Follmer, E E Almeida-Amaral, E Fialho","doi":"10.1590/1519-6984.293237","DOIUrl":"https://doi.org/10.1590/1519-6984.293237","url":null,"abstract":"<p><p>We investigated the effect of extracts of the germinated seeds of yellow soybean cultivar BRS 258 (Glycine max [L.] Merrill) against Leishmania amazonensis promastigotes. The yellow soybean embryos extract (YSEE) was fractionated on a Sephacryl S-100 gel filtration column coupled to AKTA. Then, the fractions were applied on a 1D SDS-PAGE gels and analyzed using a Micromass ESI-Q-ToF mass spectrometer coupled to a NanoUPLC. The anti-Leishmania properties of the soybean and the Jack bean (Canavalia ensiformis) urease were evaluated by measuring promastigotes mitochondrial activity using the MTT method. The YSEE reduced significantly L. amazonensis promastigotes cell viability (94.9%) and this inhibition can be related to an embryo-specific urease. We suggest that this enzyme was able to reduce the cell viability of L. amazonensis, since an anti-Leishmania activity was confirmed with an isolated urease of the legume Jack bean. The results suggest a possible relationship of soybean embryos with urease and leishmanicidal activities. Up to now, no data were found in the literature that demonstrates an anti-Leishmaniaactivity from an embryo-specific urease of soybean.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293237"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External and internal architecture of nests of fungus-growing ants of the genera Cyphomyrmex, Mycetarotes, Mycocepurus, Myrmicocrypta, and Trachymyrmex (Hymenoptera: Formicidae).","authors":"A A Moreira, O C Bueno, R S Camargo, M A Castellani, S A Leite, J C Zanuncio, J A Sabattini, R C A L Paula, R N S Lemos, B S Coelho, N C Oliveira","doi":"10.1590/1519-6984.294155","DOIUrl":"https://doi.org/10.1590/1519-6984.294155","url":null,"abstract":"<p><p>Studying the nest structures of fungus-growing ants of the genera Cyphomyrmex, Mycetarotes, Mycocepurus, Myrmicocrypta, and Trachymyrmex is essential to understanding their ecological role. Structural variations affect adaptation of these ants and their impact on agriculture and forest ecosystems. The internal and external structures of 49 fungus-growing ant nests were marked over three months, with four, seven, 31, four, and three nests, respectively, of the genera Cyphomyrmex, Mycetarotes, Mycocepurus, Myrmicocrypta, and Trachymyrmex (Hymenoptera: Formicidae: Attini) were described. The study were studied in the João Dias da Silveira Campus (Bela Vista) of the São Paulo State University (UNESP) in Rio Claro, São Paulo state, Brazil (22°23'73\" S and 47°32'53\" W). Forty-nine nests were marked over three months, with four, seven, 31, four, and three nests, respectively, of the genera Cyphomyrmex, Mycetarotes, Mycocepurus, Myrmicocrypta, and Trachymyrmex Four spherical chambers were observed in Cyphomyrmex nests, with height, width, length, and depth, respectively, of 2.8 cm, 2.5 cm, and 2.1 cm, and 15 cm to 30 cm. A single spherical chamber at a depth from 10 to 25 cm was observed in a Mycetarotes sp. nest and in each of the 31 nests of Mycocepurus sp. and Myrmicocrypta sp., with height, width, length, and depth, respectively, of 3.6 cm, 4.5 cm, and 4.1 cm, and 10 to 110 cm; and 5 cm, 5.9 cm, 6.3 cm, and 10 cm to 72 cm. The number of fungus chambers ranged from one to four per Trachymyrmex sp. nest, at depths between 15 cm and 205 cm and height, width, and length, respectively, of 3.9 cm, 7.2 cm, and 7.9 cm. Variations in the internal and external structures of Attini ant nests indicate that they should be considered to understand the ecology of these insects.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e294155"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollination efficiency by bee guilds (Hymenoptera: Apoidea) to annatto flowers, Bixa orellana L.1753 (Bixaceae).","authors":"K S Arteman, M A Uchoa, V V Alves-Junior, E A B Almeida, M R Maestre","doi":"10.1590/1519-6984.292494","DOIUrl":"https://doi.org/10.1590/1519-6984.292494","url":null,"abstract":"<p><p>Insect's pollination is essential in the maintaining of species abundance and diversity in the biomes. Bees are the most common pollinators of flowering plants, being their services very important in the conservation of biotope and biodiversity. The aims of this paper, are: to know the bee diversity and their population patterns over annatto flowers in the orchard; evaluate seed production in annatto plants with and without entomophilous pollination; to compare whether body size and intertegular distance of bees visiting annatto flowers correlates, and whether size of floral visitor bees in annatto influence in the pollination and productivity. During a flowering season in Dourados, Mato Grosso do Sul state, Brazil, 240 flower buds were chosen from five different plants and divided into six groups. In each group had 40 flower buds in the pre-anthesis phase (treatments): T1 (control), T2 (natural self-pollination), T3 (Anemophilous pollination), T4 (Big bees), T5 (Medium bees) and T6 (Small bees). Bee species were identified and measured by two metrics: body size (BS) and intertegular distance (ITD) to categorize their guilds. The treatments considered in the population's analyses were the following: species richness, constancy, frequency, dominance of each bee species, and if bee size would influence in the process of annatto pollination. The analysis of variance was determined by the Tukey and Kruskal-Wallis tests (P < 0.05) for the population parameters. Floral visitor bee species in B. orellana were divided into two groups according to the periods of their activity: 6h-9h and 14h-18h, and in four guilds, based in their body size. Bees recorded from 6h to 9h were responsible for higher efficiency in the pollination rate; represented by the following species: Bombus morio, Centris sponsa, Centris fuscata; Epicharis flava, Epicharis affinis, Epicharis analis, Euglossa sp., Eulaema nigrita, Geotrigona mombuca, Melipona quadrifasciata, Hylaeus sp., and Pseudaugochlora graminea. Seed production was higher in the control (free access to all combined floral visitor), followed by big bees, and medium bees. The two metrics: (BS and ITD) has significant positive correlation. Big body species were more successful in the pollination of B. orellana.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292494"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment of wheat flour with microelements and bran: improving the quality of bakery products and enhancing their nutritional value.","authors":"Zh Ussenova, A Tulekbaeva, A Chulenyov","doi":"10.1590/1519-6984.292477","DOIUrl":"https://doi.org/10.1590/1519-6984.292477","url":null,"abstract":"<p><p>This research aims to develop recommendations for enriching wheat flour with micronutrients to improve the nutritional value of the product and contribute to population health. To achieve this goal, testing was conducted on flour enriched with micronutrients such as iron, potassium iodide, folic acid, and Vitamin B12, and with the addition of finely dispersed bran (experimental sample) as well as non-enriched flour (control sample). The results indicate that the enrichment of flour with micronutrients and bran leads to significant changes in its composition and characteristics. The experimental flour sample was found to be richer in gluten by 10.7% compared to the control sample. The nutritional value of enriched flour also increased, surpassing the nutritional value indicators of regular flour by 6.5%. The organoleptic analysis of bread made from this flour showed positive ratings in terms of appearance, aroma, taste, and crumb structure, making such products more appealing to consumers and providing a rich and diverse taste. Thus, the enrichment of flour with micronutrients and bran presents an interesting perspective for creating products with enhanced nutritional value and unique organoleptic characteristics, but it requires careful control and adaptation in the cooking process.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292477"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofertilization with beneficial microorganisms and low rates of chemical fertilization in potatoes (Solanum tuberosum L.) management for sustainable agriculture.","authors":"R H Tirado-Malaver, R Tirado-Lara","doi":"10.1590/1519-6984.286059","DOIUrl":"https://doi.org/10.1590/1519-6984.286059","url":null,"abstract":"<p><p>Potato is considered one of the most important crops in the world. However, biotic and abiotic factors can considerably affect the production of this crop and increase production costs, the biofertilizers are environmentally friendly alternatives that could reduce these problems. The objective was to determine the biofertilizer activity of Trichoderma harzianum and Bacillus subtilis as a sustainable alternative to reduce chemical fertilization in potato productivity. In this research, two potato varieties (commercial variety Yungay and the improved variety 302295.32), two beneficial microorganisms (Trichoderma harzianum and Bacillus subtilis both at a dose of 1 kg ha-1) and three doses of chemical fertilization (20, 50 and 100% NPK) and a control (commercial variety Yungay at a dose of 100% NPK) were used in a 2x2x3+1 factorial scheme. The findings obtained reported that the use of the variety CIP302295.32 biofertilized with T. harzianum can obtain similar results using less chemical fertilizers compared to high NPK doses. The highest biometric parameters in potato were achieved in the treatments, variety CIP 302290.11 inoculated with T. harzianum at doses of 50 and 100% of NPK, so that at a dose 50% less fertilizer produced an increase of 24. 39% in the number of tubers per plant compared to the control and in the number of commercial tubers per plant the increase was 50.31%. This same treatment had a significant effect on yield parameters, increasing by 42.17% in total yield and 49.09% in commercial yield compared to the control. Therefore, this research demonstrated that biofertilization of the improved variety 302295.32 with T. harzianum at a dose of 50% NPK is a valuable alternative to increase the yield of sustainable potato cultivation in Peruvian Andean.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e286059"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}