Brazilian Journal of Biology最新文献

{"title":"First record of Crepidobothrium sp. (Cestoda, Proteocephalidae) in Bothrops atrox (Serpentes, Viperidae) in Amazonian Brazil.","authors":"A C O Sarmento, D Chagas-de-Souza, T Alves-Coêlho, L L Corrêa, A P Santos-Jr","doi":"10.1590/1519-6984.291999","DOIUrl":"https://doi.org/10.1590/1519-6984.291999","url":null,"abstract":"<p><p>The objective of this study was to document the infection by cestodes Crepidobothrium sp. in Bothrops atrox, which are from the Tapajós National Forest, located in the western part of Pará state, Brazil. Thirty-six specimens of B. atrox were examined, of which 13 individuals (36%) were infected by Crepidobothrium sp. and a total of 147 parasites were collected from these hosts. The mean abundance of these endoparasites was 4.1 and the mean infection intensity was 11.3. In relation to parasitic infection sites, 88.4% of these cestodes were found in the small intestine, 9.52% in the large intestine and 2% in the stomach of the hosts. This was the first report of Crepidobothrium sp. in B. atrox in the Brazilian Amazon.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291999"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of fungal species in bats from Brazil's Atlantic Forest.","authors":"B G Furtado, G D Savi-Bortolotto, T S Soto, L O Rocha, K P Supi, E Angioletto, F Carvalho","doi":"10.1590/1519-6984.293859","DOIUrl":"https://doi.org/10.1590/1519-6984.293859","url":null,"abstract":"<p><p>Molecular identification of fungal species associated with insectivorous bats provides critical insights into their role as reservoirs of fungi, including potential pathogens. This is the first study to performed molecular identification of fungal species in insectivorous bats captured from the Atlantic Forest of southern Brazil. Aspergillus sydowii and Penicillium crustosum were frequently isolated from Myotis sp., with P. crustosum also dominating samples from Molossus molossus, alongside Aspergillus subalbidus. Cladosporium spp. were prevalent in Eptesicus diminutus, while Sarocladium was uniquely associated with Tryroptera tricolor. Notably, several identified species are known pathogens of humans, animals, and plants. Fungal species in bats remain poorly understood, representing a significant gap in the field of fungal ecology. This gap is particularly notable in Brazilian biomes, where research on this topic is still scarce. Efforts should focus on minimizing habitat loss for bats and mitigating forest disturbances to reduce their interactions with humans, crops, and other animals. Such measures are essential to prevent the dispersal of fungal pathogen spores and safeguard ecological and public health.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293859"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary composition on the development and longevity of Lasioderma serricorne (Fabricius, 1792): implications for pest management in stored products.","authors":"G S Oliveira, D Cortese, M G Fernandes","doi":"10.1590/1519-6984.285151","DOIUrl":"https://doi.org/10.1590/1519-6984.285151","url":null,"abstract":"<p><p>Lasioderma serricorne (Fabricius, 1792) (Coleoptera: Anobiidae) is a cosmopolitan pest of stored products. Its development and longevity are highly influenced by diet, making the understanding of this relationship critical for the development of effective pest control strategies. This study explores the effects of different dietary substrates on the development and longevity of L. serricorne, using eight food substrates of both economic and nutritional significance. These substrates include rice, popcorn, rice flour, oats, wheat, corn, wheat flour, and a flour mix of cornmeal, wheat germ, and brewer's yeast in a 5:2:1 ratio. In the experiment, 50 g of each substrate were infested with ten pairs of adult insects. Cultures were maintained under controlled conditions with daily monitoring of insect emergence for 90 days. Adult longevity was assessed after emergence by transferring individuals to Eppendorf tubes, where they were monitored for survival. Results showed significant differences in insect emergence and longevity across substrates, with rice and wheat flours supporting the highest emergence rates and wheat providing the longest adult longevity. The flour mix was found to be the least suitable for insect development. These findings underscore the importance of dietary composition in the biology of L. serricorne and provide insights for the development of targeted pest management strategies for stored products.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e285151"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discrimination between wild type and heterozygous piebald ball python (Python regius) by PCR and qPCR.","authors":"R Kumsiri, N N Yin, P Kanchanaphum","doi":"10.1590/1519-6984.293718","DOIUrl":"https://doi.org/10.1590/1519-6984.293718","url":null,"abstract":"<p><p>The ball python, also known as Python regius, is a popular exotic pet due to its beautiful colors and patterns, particularly the piebald variety. However, it can be challenging to distinguish between the wild type and heterozygous piebald (het piebald) phenotypes. This study employs PCR and qPCR techniques to differentiate between these two genotypes. The PCR product size for both the wild type and het piebald was 255 bp. However, the intensity of the PCR product from the wild type was twice that of the het piebald, and no PCR product was detected in the piebald individuals. Additionally, the Ct value for the wild type was lower than that of the het piebald. The qPCR analysis revealed a 15.45% difference between the two groups. Therefore, PCR and qPCR effectively distinguish between the wild type and het piebald genotypes of ball pythons.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293718"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics analysis and chemical profiling of a decoction Tristaniopsis Merguensis leaf as bio supplement for antioxidant, and anti-cholesterols.","authors":"S Agustini, L P Manalu, N Lestari, W Purwanto, Nasruddin, H Widodo, A Asmaliyah, S H Saputra, Nilawati, G Cahya","doi":"10.1590/1519-6984.291658","DOIUrl":"https://doi.org/10.1590/1519-6984.291658","url":null,"abstract":"<p><p>Hyper cholesterol and oxidative stress are major risk factors for the onset of cardiovascular diseases. The T. merguensis has been stated to comprise a superior antioxidant substance that could stipulate beneficial advantages, and traditionally applied as herbal medicine for hyper cholesterols. The aim of the study was to evaluate of decoction leaf extracts from T. merguensis for their cholesterol reduction effect in vitro, anti-microbial activity, antioxidant activity, flavonoids and total phenolics contents and its metabolite composition. The test results show that the water decoction extracts have a high value of total phenolic content, and flavonoids. Metabolome analysis and chemical profiling by using liquid chromatography-high resolution mass spectroscopy (LC-HRMS) leads to 30 compounds are identified in the extracts. The compounds contained in the extracts are non-flavonoid polyphenols, including 18 compounds (66.75%), followed by flavonoid polyphenols (4 compounds/17.20%), alkaloids (3 compounds/5.77%), and terpenes (1 compound/5.54%), and one protein recombinant (NP-003141/4.74%).The major compounds are recognized as quinic acid, 1,6-bis-O-(3,4,5-trihydroxybenzoyl) hexopyranose, myricitrin, citric acid, (-)-Caryophyllene oxide, NP-003141, myricetin, 2-Amino-1,3,4-octadecanetriol, ellagic acid, and sedanolide. The decoction leaf extracts show robust antioxidant activity in DPPH assays, with IC50 values of 15.64 µg/g. It also shows appreciable ferric-reducing activity and ABTS antioxidant capacity of 118.9 mM Fe (II)/mg and 7463.3 mM TE/ g. It also possesses anti-cholesterols properties in vitro; antimicrobial activity on S. aureus and S. epidermis at concentration of 15,000 ppm. This study concludes that decoction leaf extracts of T. merguensis is suggested as an organic bioactive basis, with anti-cholesterol and antioxidant capabilities. In addition, in vitro and in vivo studies are needed to confirm this work.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291658"},"PeriodicalIF":0.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological development, calcium dynamic and metabolite profile of stress-induced tobacco (Nicotiana tabacum L.) microspore under calcium chloride treatment.","authors":"C T Rahmawati, D K Wahyuni, H Purnobasuki, N K K Ikram, S Subramaniam, S P O Werbrouck, Hamidah","doi":"10.1590/1519-6984.291533","DOIUrl":"https://doi.org/10.1590/1519-6984.291533","url":null,"abstract":"<p><p>Exogenous calcium is pivotal in activating microspore embryogenesis via calcium signaling. Altering the external calcium levels in the medium has been documented to impact the success rate of microspore embryogenesis initiation. However, the cellular effects on the formation of embryogenic tobacco microspore and its cellular changes during stress treatment remain elusive. We examined microspore cytological development, specifically vacuolation types and first division pattern. We also used Fluo 3-AM staining to observe the calcium dynamics, including accumulation and distribution of intracellular calcium ions (Ca2+) under fluorescent microscope. Metabolomic analysis of induced microspores was carried out using gas chromatography-mass spectrometry (GC-MS). The result showed that at 2.0 mM CaCl2.2H2O treatment, 57.06 ± 6.95% of microspores exhibited star-like structures, and 47.39 ± 8.84% underwent symmetric first division, twice as effective as 1.0 mM. Fluo 3-AM staining revealed increased Ca2+ fluorescence on day one, persisting in 2.0 mM treatment by day three. By day six, fluorescence in 1.0 mM and 2.0 mM treatments significantly differed. Intracellular Ca2+ signals rose with 1.0 mM CaCl2.2H2O and fell with 2.0 mM CaCl2.2H2O. The metabolomic analysis detected 34 metabolites, including amino acids, organic acids, and fatty acids, with predominant oleic acid, palmitic acid, and stearic acids. We conclude that two millimolar of calcium chloride was optimum for inducing embryogenic microspore formation in tobacco. Our findings underscore the significant role of exogenous calcium in microspore embryogenesis, elucidating its impact on cellular dynamics and metabolite profiles. These insights contribute to hold potential implications for enhancing crop breeding strategies.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291533"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing growth and survival rate of Asian Redtail Catfish Hemibagrus nemurus (Valenciennes 1840) Larvae through MinaPro probiotic soaking.","authors":"S Sukendi, R M Putra, E Efriyeldi, D Andriani, R K Sinaga","doi":"10.1590/1519-6984.291745","DOIUrl":"https://doi.org/10.1590/1519-6984.291745","url":null,"abstract":"<p><p>Asian redtail catfish (Hemibagrus nemurus) is a freshwater fish commodity with high economic value. However, the growth of the catfish larvae into fry is relatively slow. The administration of probiotics as a solution can increase the growth of fish larvae. Therefore, this research aimed to analyze the use of probiotics with a larval immersion method on the growth and survival of Asian redtail catfish larvae. A completely randomized design was adopted with four treatments and three replicates. Immersion treatment using probiotics consisted of P0 (control treatment without probiotic immersion), P1 (immersion treatment with a dose of 2.0 ml.L-1 of water), P2 (immersion treatment with a dose of 4.0 ml.L-1 of water), and P3 (immersion treatment with a dose of 6.0 ml.L-1 of water), with a stocking density of 50 fish/aquarium. The immersion was carried out every 10 days for 10 minutes in a basin container using 1 liter of water according to the specified dose. The results showed that the immersion larvae in probiotics significantly affected the biometric growth and survival (p<0.05). The highest biometric growth and survival was in P3- the group of immersion treatment with a dose of 6.0 ml.L-1 of water. This obtained absolute weight, length, and specific growth of 7.04 g, 8.81 cm, and 11.37% per day, as well as cannibalism type A and B of 8.00% and 0.00%, with cannibalism index, normal mortality, survival rate of 8.00%, 3.33%, and 88.67%, respectively.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291745"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanins in relation to nutrients, stomatal density, and yield of purple lettuce (Lactuca sativa L.) fertilized with compost based on bleaching earth.","authors":"D D Cruz Nieto, N E Muguruza Crispín, E T Castañeda Chirre, J U Romero Menacho, M I Martinez Loli, C P Alvarez Quinteros, J C Cabanillas Sulca, O O Osso Arriz, F V Luperdi Rios, P M Quispe Barrantes, M V Bautista Espinoza, J R Reaño Rivera, G C Pesantes Arriola, C M Caira Mamani, M N Morales Alberto, A Chiclla Salazar","doi":"10.1590/1519-6984.291967","DOIUrl":"https://doi.org/10.1590/1519-6984.291967","url":null,"abstract":"<p><p>In Peru, in recent years, the gastronomic boom has stood out globally, which has increased the production of edible oil in the oil industries, generating too much bleaching land. This study investigated anthocyanins in relation to nutrients, stomata density and yield of purple lettuce nourished with bleaching soil-based compost. The objective was to analyze the concentration of anthocyanins as a function of nutrients, stomata density and yield of purple lettuce nourished with such compost. It is based on a methodology applied with an experimental approach; for which the statistical model of the Completely Randomized Block Design was used, consisting of 3 blocks and 5 treatments which were T1 with 0, T2 with 6, T3 with 8, T4 with 10 and T5 with 12 t/ha of compost based on bleaching soil and the doses were applied 15 days after transplanting. Total plant length, plant height, plant weight, commercial yield, and equatorial diameter were evaluated. Additionally, the concentration of nutrients in leaves (Nitrogen, potassium, phosphorus, calcium, magnesium, sulfur, molybdenum, iron, manganese, copper, zinc, boron, chlorides, and sodium), anthocyanin, stomatal density, and profitability. The results determined that T5 stood out in total plant length with 32 cm, plant height with 27 cm, plant weight with 200.27 g, commercial yield with 23.57 t/ha and equatorial diameter with 16.17 cm. Total nitrogen supply was 255.1 kg/ha. Nutrient concentrations in leaves were phosphorus, sulfur, molybdenum, manganese, copper, zinc and boron. Stomata density in leaves with 118 stomata/mm2. Profitability with 129% and anthocyanin concentration in T2 leaves with 89.25 mg/100g. It is concluded that adequate anthocyanin concentration in T5, with 62.86 mg/100 g, influenced the concentration of most nutrients and greater density of stomata in the leaves, acting as a defense mechanism against nutritional and environmental stress, obtaining higher yields, 30% higher compared to the control.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291967"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complementary effect of melatonin in accelerating ovulation and spawning of the Javaen barb Systomus rubripinnis (Valenciennes 1842).","authors":"A O Sudrajat, D Radona, A Alimuddin, W Manalu, O Carman, R R S P S Dewi, I I Kusmini, T H Prihadi, B Pantjara, E K Kontara","doi":"10.1590/1519-6984.291024","DOIUrl":"https://doi.org/10.1590/1519-6984.291024","url":null,"abstract":"<p><p>The Javaen barb Systomus rubripinnis is an endemic fish not domesticated in Indonesia. This study aims to evaluate the effectiveness of gonadotropin and melatonin hormones in inducing ovulation of female Javaen barb. A total of 12 female fish (BW: 142.12 ± 18.08 g; egg diameter 1.0-1.2 mm) were selected to be injected with a combination of different hormones. The treatments were ovaprimTM at a dose of 0.6 mL/kg without melatonin (L0.6M0), ovaprimTM at 0.3 mL/kg with 0.25 mg/kg melatonin (L0.3M0.25), 500 IU/kg of human chorionic gonadotropin with 0.25 mg/kg melatonin (H500M0.25), and 0.25 mg/kg melatonin (M0.25). The L0.6M0 and L0.3M0.25 treatments were injected twice. The first injection was 40% of the total dose, while the rest (60%) was injected 6 hours after the first injection. Melatonin injection was carried out at the same time as the first injection. In the H500M0.25 treatment, melatonin injection was carried out 24 hours after HCG injection. Fish injected with ovaprimTM with and without melatonin had the fastest latency period, and ovulation occurred in all fish. The H500M0.25 treatment had an ovulation rate of 66.7%, while those injected with only melatonin (M0.25) did not ovulate. The number of ovulated eggs, fertilization and hatching rate from ovaprimTM injected broodstock were higher than those of HCG. In contrast, the larvae's survival rate, body weight, and length were similar. In conclusion, ovaprimTM is practical in inducing ovulation of Javaen barb, and melatonin has a complementary effect on Javaen barb ovulation.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291024"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon footprint assessment of livestock farms under tropical conditions: first approximation.","authors":"S A Guamán-Rivera, F M Carrillo Riofrío, M E Jativa-Brito, L E Chuqui-Puma, G M Soldado Soldado, L D Cabezas Andrade, H R Sanchez Quispe, A A Casierra Cardenas, J M Mira Naranjo, J P Santillán Aguirre, C D Congo-Yépez","doi":"10.1590/1519-6984.293349","DOIUrl":"https://doi.org/10.1590/1519-6984.293349","url":null,"abstract":"<p><p>This study evaluates the carbon footprint (CF) of livestock farms in the Orellana Province of the Ecuadorian Amazon, focusing on emissions from enteric fermentation, manure management, and energy use. Utilizing Tier I and Tier II methodologies outlined by the IPCC (2006), the research quantifies greenhouse gas (GHG) emissions across three distinct typologies of Agricultural Productive Units (APUs): Conventional Systems (Type 1), Efficient Resource Use (Type 2), and Subsistence and Dependency Systems (Type 3). Results show that the average CF ranged from 14.5 to 18.3 kg CO2-eq per kg of live weight, with enteric fermentation accounting for 60.2% of emissions, followed by manure management (25.4%) and energy use (14.4%). Type 1 farms demonstrated the highest total CF (2.400 kg CO2-eq/farm), primarily due to larger herd sizes (9.8 TLU) and less efficient practices. Type 2 farms, characterized by rotational grazing and improved resource use, showed a reduced CF of 1.860 kg CO2-eq/farm. Type 3 farms, though smaller in scale, exhibited a CF of 1.810 kg CO2-eq/farm, with higher emissions intensity per animal (60.3 kg CO2-eq/animal) compared to Type 1 and 2 (53.2 kg CO2-eq/animal on average). Across all typologies, methane (CH4) from enteric fermentation remains the largest emission source. The findings underscore the critical need for sustainable interventions, such as silvopastoral systems, to mitigate GHG emissions while improving productivity. This research provides baseline data for designing region-specific mitigation strategies, contributing to global efforts to reduce the environmental impact of livestock farming.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293349"},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}