F X Quintino Neta, W P Macedo, F G Jesus, E C Rocha, C M Silva-Neto, M S Araújo
{"title":"Bioactivity and selectivity of novel aminoaryl-phosphoramidates against target (Spodoptera frugiperda) and non-target insects (Polybia occidentalis).","authors":"F X Quintino Neta, W P Macedo, F G Jesus, E C Rocha, C M Silva-Neto, M S Araújo","doi":"10.1590/1519-6984.301647","DOIUrl":"10.1590/1519-6984.301647","url":null,"abstract":"<p><p>Phosphoramidates are a class of chemical compounds still widely used for insect pest control; therefore, in this group, there is an underlying need to investigate novel insecticide compounds. This study evaluated the insecticidal efficacy and physiological selectivity of three novel aminoaryl-phosphoramidate compounds under controlled laboratory conditions. The biological models were larvae of the phytophagous Spodoptera frugiperda and adult workers of the predatory Polybia occidentalis. The S. frugiperda larvae used for the bioassays were reared on an artificial diet, while P. occidentalis adults were collected from field colonies. The acute larvicidal efficacy of the novel phosphoramidate compounds against S. frugiperda larvae was lower than that of the control treatment (a commercial product of the same chemical class); however, these same compounds exhibited significant acute toxicity to workers of P. occidentalis. Following topical application of phosphoramidate compounds, insects were provided an ad libitum feeding diet, and toxicity associated with potential delayed neurotoxic effects was assessed under these conditions. The LD50 (lethal dose killing 50% of the tested population) of the three aminoaryl-phosphoramidate compounds ranged from 28.10 to 99.94 μg mg-1 for S. frugiperda larvae and from 54.86 to 68.71 μg mg-1 for adult P. occidentalis adults. The synthesized compound exhibits more pronounced acute and chronic effects on the non-target insect (P. occidentalis) and a less pronounced rapid lethal effect on S. frugiperda compared to commercial insecticides, indicating undesirable properties. However, its notable delayed neurotoxic effect to S. frugiperda, which is understudied for compounds of this nature, could be further explored as an additional tool for pest control.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e301647"},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147693854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D R Silva, M A A Medeiros, A R S Rodrigues, V G S Rêgo, R M Anjos, A A Oliveira Filho
{"title":"Antibacterial potential of Citrus sinensis essential oil against foodborne pathogens: a systematic review.","authors":"D R Silva, M A A Medeiros, A R S Rodrigues, V G S Rêgo, R M Anjos, A A Oliveira Filho","doi":"10.1590/1519-6984.304325","DOIUrl":"10.1590/1519-6984.304325","url":null,"abstract":"<p><p>Bacteria are single-celled, prokaryotic organisms that can be present in contaminated food, causing various waterborne and foodborne diseases (WBD), which generate high morbidity rates and, in more severe cases, mortality in various regions of the world. The growing increase in bacterial resistance to antimicrobials used in the food industry and public health represents a serious health challenge, making the search for new microbial control strategies urgent. In this context, natural products, such as essential oils, have gained prominence for their bioactive compounds with significant antimicrobial potential, such as the terpenes found in Citrus sinensis (sweet orange) essential oil, which have been shown to be effective against various pathogenic bacteria involved in WFDIs. The objective of the study was to conduct a systematic review to evaluate the therapeutic potential of Citrus sinensis essential oil against foodborne pathogenic bacteria. The search covered documents in Portuguese and English published between 2019 and 2024. Data were collected through CAPES electronic journal platforms, using the Embase, PubMed, and Web of Science databases. The studies were organized and managed with the help of Mendeley software. Initially, 1,562 articles were found. After applying exclusion criteria and refining the search, 12 studies were selected for this review. It was observed that Citrus sinensis essential oil, both alone and in combination with other compounds, demonstrated significant antibacterial activity against gram-positive and gram-negative strains. Citrus sinensis essential oil appears to be a promising alternative in combating pathogenic bacteria, although further studies are needed to elucidate its mechanism of action and broaden its spectrum of application.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e304325"},"PeriodicalIF":0.0,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial bioactive groups for the improved and stable functionality of nanoparticles for biomedical application.","authors":"Suhailah S Aljameel","doi":"10.1590/1519-6984.295953","DOIUrl":"10.1590/1519-6984.295953","url":null,"abstract":"<p><p>Across the world, metal nanoparticles (NPs) have received immense attention due to their vast array of applications in the biomedical field. This developing branch of nanotechnology, where the particles expand in nano size, is evolving for the pharmaceutical field in a more responsive manner. In recent years, green synthesis/nanotechnology has developed as a watchword for the fabrication and synthesis of metal NPs. In the present article, the perspective and progress achieved in recent years on the role of bacterial bioactive functional molecules during biosynthesis have been discussed. The cascades for NPs synthesis, manipulation of the number of biological metabolites as the reducing and stabilizing agents, like enzymes, proteins, redox mediators, and exopolysaccharides, have been presented. Therefore, the article elucidates the involvement of microbial metabolites to harmonize the biosynthetic NPs and the possible mechanism leading to biosynthesis of commonly studied and utilized metal NPs like silver, gold, zinc, copper, magnetite, palladium, etc., and their immense potential in biomedical applications.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295953"},"PeriodicalIF":0.0,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Bunsueb, C Taoto, S Tongpan, C Chaimontri, S Iamsaard
{"title":"Restorative effects of Thai Mucuna pruriens seed extract on reproductive, hormonal, and fertility impairments in letrozole-induced PCOS rats.","authors":"S Bunsueb, C Taoto, S Tongpan, C Chaimontri, S Iamsaard","doi":"10.1590/1519-6984.303146","DOIUrl":"10.1590/1519-6984.303146","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) is a complex endocrine disorder, leading cause of female infertility, disrupted estrous cycles, hormonal dysregulation, and abnormal ovarian morphology. This study aimed to evaluate the restorative effects of Thai Mucuna pruriens (TMP) seed extract on reproductive, hormonal, and fertility impairments in a PCOS rat model induced by letrozole. Following PCOS induction, female rats received TMP extract at 150, 300, and 600 mg/kg BW for 30 consecutive days. Untreated PCOS rats displayed persistent diestrus, hormonal dysregulation, and polycystic ovaries, and no pregnancies were observed. After TMP treatment, especially at 300 mg/kg BW, significantly reversed these impairments: estrous cyclicity normalized, hormonal profiles improved (increasing FSH and estradiol, decreasing LH and testosterone levels), and ovarian histology showed active folliculogenesis and reappearance of multiple corpus lutea. In addition, TMP could reduce the body weight and downregulate the ovarian androgen receptor (AR), estrogen receptor alpha (ERα), and tumor necrosis factor-alpha (TNF-α) protein expression. Moreover, TMP restored the fertility index and promoted embryo implantation, comparable to control animals. In conclusion, Thai Mucuna pruriens seed extract, particularly at 300 mg/kg, effectively reversed the reproductive, endocrine, and fertility impairments in letrozole-induced PCOS animal model, suggesting its promising potential as a phytotherapeutic agent for managing PCOS-associated reproductive dysfunction.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e303146"},"PeriodicalIF":0.0,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Ametov, M Dauletbayeva, M Aytzhanova, Z Rakhimova, A Sarsembayeva, U Erezhetova, Zh Turgara, G Bolatbekova, A Bekenova, G Assan
{"title":"Comparative phytochemical and GC-MS analysis of leaves, stems, and roots of Euphorbia yaroslavii Poljakov.","authors":"A Ametov, M Dauletbayeva, M Aytzhanova, Z Rakhimova, A Sarsembayeva, U Erezhetova, Zh Turgara, G Bolatbekova, A Bekenova, G Assan","doi":"10.1590/1519-6984.304072","DOIUrl":"10.1590/1519-6984.304072","url":null,"abstract":"<p><p>Euphorbia yaroslavii Poljakov. is a rare plant species endemic to Kazakhstan whose phytochemical composition has remained largely unexplored. This study presents the first organ-specific phytochemical and GC-MS characterization of the leaves, stems, and roots of this species. Quantitative analyses indicated pronounced metabolic differentiation among plant organs. Roots were characterized by high contents of total phenolics (58.2 ± 2.5 mg GAE/g DW), coumarins (2.48%), and mineral components (total ash 13.22%), while leaves exhibited the highest tannin content (2.35%). The maximum flavonoid content was observed in roots (0.27%), whereas stems contained the highest level of ascorbic acid (0.70 mg/g DW). GC-MS profiling tentatively identified 68 compounds in leaf extracts, 65 in stem extracts, and 39 in root extracts. Root extracts were dominated by fatty acids and triterpenoid-sterol constituents, including octadecanoic acid (18.9%), hexadecanoic acid (17.5%), β-amyrin (5.2%), and lanosterol (4.9%). Overall, the results indicate organ-specific metabolic specialization in Euphorbia yaroslavii Poljakov. and provide a reliable phytochemical baseline for future bioactivity-oriented and conservation-related studies.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e304072"},"PeriodicalIF":0.0,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E M Sousa, N D Alves, M S Araujo, A S Pimenta, E C Silva Junior, C S Santos, F M C Feijó
{"title":"Sanitization of eggs using eucalyptus wood vinegar.","authors":"E M Sousa, N D Alves, M S Araujo, A S Pimenta, E C Silva Junior, C S Santos, F M C Feijó","doi":"10.1590/1519-6984.295526","DOIUrl":"10.1590/1519-6984.295526","url":null,"abstract":"<p><p>Disinfection is essential for egg production. Bacterial resistance to conventional disinfectants such as chlorine has led to research into alternative substances such as eucalyptus wood vinegar. Thus, the objectives are to characterize the physical, chemical, and microbiological aspects of chicken eggs when using 1% eucalyptus wood vinegar. The study analyzed eggs submitted to three treatments (water, 1% chlorinated solution, and 1% wood vinegar). The quality of the eggs was evaluated through physical-chemical analyses, observing the internal and external aspects of quality (Haugh unit, albumen, yolk, shell, and weight). In the microbiological analysis, we used a sample of Salmonella enterica serovar Enteritidis ATCC® 13076 in eggs and passers at UFERSA, including students, employees, and professors, to verify the taste of the eggs when using the 1% eucalyptus wood vinegar. The analyses showed that using the eucalyptus wood vinegar did not interfere with any physical aspects apart from specific gravity and decreased the number of bacteria in eggs. In conclusion, wood vinegar's antimicrobial action enables its application to sanitize eggs, replacing chemical products with a high environmental impact.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295526"},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F A M Corrêa, F R C da Silva, A E Piazentine, A L Bacha, P L C A Alves
{"title":"Sugarcane's chemical ripeners can stimulate growth of young coffee plants in a drift scenario.","authors":"F A M Corrêa, F R C da Silva, A E Piazentine, A L Bacha, P L C A Alves","doi":"10.1590/1519-6984.300084","DOIUrl":"10.1590/1519-6984.300084","url":null,"abstract":"<p><p>Due to the proximity between sugarcane and coffee areas in Brazil, studies are needed to evaluate the effect of different chemical ripeners in a possible drift scenario. Two open-area experiments were carried out to evaluate the effects of sugarcane's sector commonly used ripeners on young coffee plants. In the first experiment, four ripeners were tested: glyphosate, sulfometuron-methyl, trinexapac-ethyl and ethephon, plus a control without application. The second experiment was conducted with increasing doses of the one that caused the greatest damage to the crop (glyphosate). Coffee growth and physiological parameters were assessed. For the first experiment, glyphosate (180 g a.e. ha-1) provided the greatest negative effects on coffee growth. The application of 250 g a.i. ha-1 of trinexapac-ethyl caused a positive effect on plant growth. Complete hormesis was observed on coffee plants, in which glyphosate doses up to 60 g a.e. ha-1 stimulated growth and higher doses caused deleterious effects. Our results shade light into the effect of sugarcane chemical ripeners in coffee plants, especially for supporting the choice of safe ripeners to be used in areas close to newly established coffee orchards.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e300084"},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W Muslihatin, D N Rizky, T Nurhidayati, N Jadid, K I Purwani, A R Amna
{"title":"Gender determination of date palm (Phoenix dactylifera) seedlings through phenotypic characteristics and SRY gene with ISSR marker as positive control.","authors":"W Muslihatin, D N Rizky, T Nurhidayati, N Jadid, K I Purwani, A R Amna","doi":"10.1590/1519-6984.302811","DOIUrl":"10.1590/1519-6984.302811","url":null,"abstract":"<p><p>The date palm (Phoenix dactylifera), a member of the family Arecaceae, is a dioecious plant in which male and female flowers occur on separate individuals. Sex determination in date palms is typically possible only at the flowering stage, which occurs at reproductive maturity between four and five years. Consequently, sex cannot be determined at the early seedling stage. This study aimed to determine the sex of date palm seedlings through phenotypic characterization and molecular analysis of the SRY gene, validated using ISSR markers. A total of 10 seedlings from each of two cultivars-KL.1 and Barhee-were used. In vivo propagation method enabled phenotypic characterization based on radicle structure (spiraled or non-spiraled) and leaf primordium morphology (curved or straight). Molecular determination of sex was conducted using SRY gene-specific primers (SRY-date F-R) and ISSR markers (IS_A02 and IS_A71). Phenotypic characterization of KL.1 resulted in the identification of seven male seedlings (K1, K3, K4, K5, K12, K14, K15) and three female seedlings (K2, K6, K9). For Barhee, phenotypic analysis indicated seven male seedlings (B1, B2, B5, B8, B11, B12, B15) and three female seedlings (B4, B6, B10). Molecular analysis of KL.1 validated the phenotypic results, with identical sex indications. In Barhee, molecular analysis identified five male and five female seedlings, showing discrepancies with the phenotypic results for two seedlings (B12 and B15). Despite these inconsistencies, Chi-square and Cramer's V tests revealed that the p-values for KL.1 (0.012) and Barhee (0.048) indicated strong and statistically significant associations between phenotypic and molecular sex determination.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"86 ","pages":"e302811"},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R N Sousa, G G Santos, G C Fernandes, S A Figueredo, L Tchaicka, J R S Barros
{"title":"DNA Barcoding applied to the identification of Meliponini in the Lençóis Maranhenses microregion, Brazil.","authors":"R N Sousa, G G Santos, G C Fernandes, S A Figueredo, L Tchaicka, J R S Barros","doi":"10.1590/1519-6984.295935","DOIUrl":"10.1590/1519-6984.295935","url":null,"abstract":"<p><p>Stingless bees (Meliponini) are essential pollinators widely distributed in tropical regions, playing a crucial role in ecosystem maintenance. In Brazil, they are found throughout the national territory. DNA barcoding has emerged as an effective tool for species identification and genetic diversity assessment, especially in areas affected by anthropogenic activities. This study aimed to identify and characterize local stingless bee species at the molecular level. A total of 32 specimens were collected from three communities in Tutóia, Maranhão (MA). Samples were processed at the LabWick/UEMA, with DNA extracted using the phenol-chloroform method and amplification of the COI region via PCR. Sequence analyses were performed using dedicated software. The mitochondrial COI marker enabled the identification of eight stingless bee species distributed across seven distinct genera. The DNA fragments analyzed averaged 450 bp in length, with a predominance of adenine (A) and thymine (T). Sequence similarity with the NCBI database ranged from 96.33% to 98.93%, with Melipona fuliginosa showing the highest match. A total of 25 haplotypes were identified, 10 belonging to the Melipona genus and 15 to other genera. Phylogenetic analysis revealed four groupings within Melipona and six among the remaining genera. The geographic isolation of the sampled communities may have limited species distribution across the study area. Despite the effectiveness of COI, the absence of a clear barcode gap and the scarcity of reference data in databases such as GenBank limit identification accuracy for certain genera. This study underscores the importance of DNA barcoding in contributing genetic data to repositories like NCBI and enhancing the known species diversity in the Lençóis Maranhenses microregion.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295935"},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L G Silva, S Carvalho-Neto, M C Souza, S M Silva, R E O Jeronimo, H F Silva, E C Silva, J G Silva, R L A Bruno, L C Nascimento
{"title":"Essential oils in the seed quality of Myracrodruon urundeuva Fr. Allemão.","authors":"L G Silva, S Carvalho-Neto, M C Souza, S M Silva, R E O Jeronimo, H F Silva, E C Silva, J G Silva, R L A Bruno, L C Nascimento","doi":"10.1590/1519-6984.299994","DOIUrl":"10.1590/1519-6984.299994","url":null,"abstract":"<p><p>Aroeira-do-sertão is a native species of the Brazilian semiarid region, with pharmacological properties and potential for reforestation of degraded areas, making the use of healthy seeds essential for seedling production. In the absence of registered chemical products for the treatment of native forest seeds, the development of effective alternative methods for pathogen control, such as the use of essential oils, becomes indispensable. The present study aimed to evaluate the effectiveness of essential oils in reducing pathogen incidence and improving the physiological quality of aroeira seeds. The experiment was conducted at the Phytopathology Laboratory of the Federal University of Paraíba. Seeds of aroeira obtained from the Seed Network of the São Francisco Integration Project were used, and their moisture content was determined. Seeds were treated with essential oils at a concentration of 1%. Treatments consisted of T1: Control; T2: Captan; T3: Cinnamon leaf; T4: Ginger; T5: Palmarosa; T6: Thyme; T7: Sicilian lemon; T8: Peppermint; T9: Oregano; and T10: Tangerine. The seeds were subjected to a health test, using ten replications of ten seeds, and to germination and emergence tests, with four replications of 25 seeds. The experiment was carried out in a completely randomized design. Cinnamon leaf and thyme essential oils reduced the incidence of the fungal genera Aspergillus, Cladosporium, Alternaria, Ulocladium, Pestalotia, and Curvularia in M. urundeuva seeds and did not affect seed physiological quality.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e299994"},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}