Brazilian Journal of Biology最新文献

{"title":"Synergistic impact of integrated mechanical, physical, and chemical disinfection on microbial ecology and morphophysiological development in dairy calves.","authors":"K Shaikenova, S Issabekova, M Sadenova, K Omarova, R Uskenov","doi":"10.1590/1519-6984.295880","DOIUrl":"10.1590/1519-6984.295880","url":null,"abstract":"<p><p>The article presents the results of studies of the influence of the complex process of cleaning and disinfection of the dispensary using mechanical, physical and chemical methods on the growth and development of calves of the dairy period. The dispensary for calves is divided into two rooms, where there were animals of the control and experimental groups selected by the method of pairs of analogues in the same feeding and maintenance conditions. Studies before the treatment of rooms for calves showed a massive growth of bacilli and bacteria - 100%, mold fungi - 80%, yeast - 20%, actinomycetes - 80%. After processing the room for calves of the experimental group, the number of microorganisms during mechanical treatment, microbial contamination decreased by 31%, during physical treatment by 62%, and during chemical treatment by 95%. Monitoring of live weight, exterior features of calves showed that the live weight of calves of the experimental group of monthly calves averaged 58.9 kg, and the control group 58.6 kg, respectively. In the second month, the live weight of the experimental was 7.5 kg or 9.1% more than the control. And also for all body measurements, the experimental group exceeded the control group by an average of 10%. The results of the study of hematological parameters showed that in the experimental group they are all within the normal range, whereas in the control group the content of leukocytes is 13.2 * 109 liters, lymphocytes are 7.7 * 109 liters higher than normal, which indicates inflammatory processes in the body of calves.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295880"},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144838614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant related to nutrition, stomatal density, and yield in cilantro (Coriandrum sativum L.) nourished with leachate from compost based on vegetable waste.","authors":"D D Cruz Nieto, N E Muguruza Crispín, J U Romero Menacho, O O Osso Arriz, B L Mamani Salcedo, F V Luperdi Rios, C P Alvarez Quinteros, J O Nicho Vargas, M I Martinez Loli, R W Dextre Mendoza, M N Morales Alberto, C M Caira Mamani, R Aguilar Anccota, G P Lacherre Rodriguez, J R Ramos Vera, M V Bautista Espinoza, G C Pesantes Arriola, J R Reaño Rivera","doi":"10.1590/1519-6984.293174","DOIUrl":"10.1590/1519-6984.293174","url":null,"abstract":"<p><p>The food crisis in Peru has intensified due to the increase in the costs of raw materials, such as energy sources, fertilizers, and other industrial inputs, as a result of global socioeconomic instability. For this reason, research was conducted on the antioxidant in relation to nutrition, stomatal density, and yield in cilantro nourished with compost leachate based on vegetable waste (CLPW). The objective was to analyze the antioxidants in relation to nutrition, stomatal density, and the yield of cilantro nourished with CLPW. The methodology was based on an applied experimental approach, so the statistical model of completely randomized block design was employed, which consisted of 3 blocks and 5 treatments: T1 with 0, T2 with 2, T3 with 3, T4 with 4, and T5 with 5 liters of CLPW per 200 liters of water. The doses were applied 15 and 30 days after planting. The data obtained from the physical characteristics of cilantro, such as total length, plant weight, number of leaves, and commercial yield, were processed using analysis of variance and the Duncan test. Additionally, the concentration of nutrients (nitrogen, potassium, phosphorus, calcium, magnesium, sulfur, molybdenum, iron, manganese, copper, zinc, boron, chlorides, and sodium), antioxidant capacity, stomatal density in leaves, and profitability were evaluated. The results determined that T5 stood out in total length with 55.23 cm, plant weight with 79.63 g, number of leaves with 162, and commercial yield with 27.017 t/ha. Regarding nutrient concentration, T5 stood out in calcium, magnesium, iron, boron, and chlorides, with a profitability of 334%. T4 stood out in antioxidant capacity with 4638.1 μmol Trolox/100 g sample, while T1 showed the highest stomatal density with 143 stomata/mm2. In conclusion, T4, with 4638.1 μmol Trolox/100 g sample, exhibited the highest antioxidant capacity. However, T5, with 4212.8 μmol Trolox/100 g sample, promoted greater nutrient absorption, lower stomatal density, and other biochemical reactions, which resulted in strengthening against environmental stress and, consequently, higher yield.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293174"},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144838651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-expression of the ICE1 gene in transgenic rice improves cold tolerance.","authors":"Z Kanagatov, G Alpamyssova, A Nagiyeva, A Yeginbay, A Alpamyssova, A Abubakirova","doi":"10.1590/1519-6984.296200","DOIUrl":"10.1590/1519-6984.296200","url":null,"abstract":"<p><p>Cold stress significantly challenges agricultural productivity, particularly in regions susceptible to low temperatures. In response to this challenge, the current study investigates the effects of over-expressing the ICE1 gene in rice plants on cold tolerance. Through constructing the pCAMBIA3301-ICE1 vector, transgenic rice lines with increased ICE1 expression were generated and investigated for their response to chilling stress. Upon subjecting the transgenic rice plants to chilling stress conditions, notable improvements were observed compared to wild-type (WT) plants. Specifically, the transgenic lines exhibited a substantial 62% reduction in visual damage severity, highlighting their enhanced resilience to cold-induced damage. Additionally, the transgenic lines showed a significant 30% increase in proline concentrations, indicative of enhanced stress tolerance mechanisms. Further analyses revealed a 50% reduction in malondialdehyde (MDA) levels and a 38% increase in peroxidase (POX) activity in the transgenic rice plants compared to the WT plants. These findings suggest improved antioxidant defense mechanisms and reduced oxidative damage, contributing to the overall cold stress tolerance of the transgenic lines. Interestingly, differential expression patterns of the ICE1 gene were observed between the leaves and roots of the transgenic plants under cold stress. Roots consistently exhibited higher levels of ICE1 expression, highlighting potential organ-specific responses to cold stress. In conclusion, the study demonstrates that over-expression of the ICE1 gene significantly enhances cold stress tolerance in rice plants; offering promising avenues for developing stress-resistant crop varieties that can better withstand the adverse effects of chilling stress on agricultural production.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e296200"},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144838652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and bioactive potential of a nanoemulsion containing bacaba peel extract.","authors":"A L Raiser, T T Hoshino, W L M Prando, M de P R Torres, B W Debiasi, A C de M Cotrim, E B Ribeiro, D M S Valladão","doi":"10.1590/1519-6984.292718","DOIUrl":"https://doi.org/10.1590/1519-6984.292718","url":null,"abstract":"<p><p>Bacaba produces fruit that are rich in bioactive compounds such as flavonoids and anthocyanins, which present antioxidant action. The processing of these fruits generates residues, like the peel, which can be used in the development of new products. Therefore, the aim was to develop a new product incorporating the bacaba peel extract in a nanoemulsified system containing munguba butter and to evaluate the stability, potential phagocytic and microbicidal activities, and cell viability. The extract was obtained by the ultrasound assisted method and was incorporated into the nanoemulsified system containing munguba butter. The formulation was evaluated for its hydrodynamic particle diameter, polydispersity index, Zeta potential, stability assays, biological assays of cell viability and phagocytosis, and microbicidal activities. The developed nanoemulsion showed to be unstable at high temperatures (45 ± 2 °C) and when exposed to light radiation and remained stable at room temperature and refrigeration for 90 days. In the biological assays, the formulation showed biocompatibility, with cell viability above 90%, on average, in addition to high phagocytic (95%) and microbicidal activities (90%), due to the presence of the extract, suggesting greater immune efficiency. Thus, the potential of using residues rich in bioactive compounds for the development of sustainable nanotechnological products, with benefits for the health of the population, is highlighted.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292718"},"PeriodicalIF":0.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of bronchoalveolar lavage in the diagnosis of pulmonary diseases and its potential clinical-laboratory applications: a review.","authors":"J M L Maux, H G Silva, M E T A Silva, G S Albuquerque, G J M Leão, I C Silva, E N T Arruda, T C N Matos, C F C Caribé, J C Silva-Neto","doi":"10.1590/1519-6984.292731","DOIUrl":"https://doi.org/10.1590/1519-6984.292731","url":null,"abstract":"<p><p>Bronchoalveolar lavage (BAL) is a non-invasive bronchoscopic technique used to aspirate cells from the pulmonary interstitium, initially applied to detect neoplastic cells but later expanded for microbiological studies, leukocyte counts, molecular analyses, and immunophenotyping. This broader approach has linked BAL to the study of interstitial lung diseases (ILD). Despite its use since the 1970s, standardization of BAL cytopathological analysis is lacking, hindering its routine clinical application. The limited number of studies, particularly in Southern Hemisphere countries, reflects challenges in refining laboratory procedures and cytological findings. This article discusses the diagnostic potential of BAL when combined with clinical and radiological data. Integrating cytomorphologic findings with molecular techniques enhances its accuracy, particularly for cases with undefined etiopathogenesis, offering improved diagnostic and follow-up capabilities. The results make it clear that BALF cytology helps tell the difference between different types of ILDs. They also show that it is a useful tool for monitoring and diagnosing lung diseases.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292731"},"PeriodicalIF":0.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of complex microbial preparation of free-living and symbiotic nitrogen-fixing bacteria for agricultural crops.","authors":"V Goginyan, S Harutyunyan, T Stepanyan, G Khachatryan, V Bagiyan, R Hovhannisyan, B Harutyunyan, M Kinosyan, N Ghazanchyan, T Davidyan, A Verdyan, K Chitchyan","doi":"10.1590/1519-6984.292171","DOIUrl":"https://doi.org/10.1590/1519-6984.292171","url":null,"abstract":"<p><p>The aim of this work was to study the newly developed biofertilizer and plant growth-promoting activity of a consortium of bacterial strains Azotobacter chroococcum MDC 6111, Paenibacillus polymyxa MDC 280 and symbiotic nitrogen-fixing bacteria Rhizobium leguminosarum MDC 5609, Mesorhizobium ciceri MDC 6048 and Bradyrhizobium japonicum MDC 5789. It should be noted that in the biofertilizer formula, the strains A. chroococcum and P. polymyxa are unaltered components, and the nodule bacteria are included depending on the type of crop, taking into account the specificity of these bacteria to plants. In this case, both the nitrogen-fixing capacity and the phosphate-mobilizing activity of these bacteria were taken into account. It was found that the increase in the organic layer content from 2.73 to 5.79% occurs with the active participation of microbial strains introduced into the soil. During the plant growth period, an increase in mobile nitrogen forms (NO3-) to 71.9 mg-eq/100 g and soil saturation with molecular nitrogen to 16.2 mg/100 g were detected. A decrease in the content of phosphorus salts (Р2О5, РО43-) and potassium (K+) to 2.86, 3.82 and 5.86 mg-eq/100 g, respectively, was shown. Thus, the content of the immobile form of potassium (K2O) decreases approximately 3 times, and the amount of immobile phosphorus (P2O5) - 3.63 times. These processes in the soil are due to the active activity of nitrogen-fixing and phosphate-solubilizing bacteria, as well as the use of these ions by plants. At the same time, the nitrogen (N) content increases by 2.38 times, and its mineralized form - NO3- by 3.0 times, respectively. As a result of the field experiments, it was found that the tested soil rhizobacteria actively adhere to seeds and seedlings, spreading in the rhizosphere of plants, contributing to the effective action of the biopreparation, thereby fixing more nitrogen. Microorganisms in the process of metabolism increase the efficiency of obtaining soluble salts of phosphorus, potassium and calcium by plants. In general, it was found that joint inoculation of rhizobacteria strains demonstrated higher growth parameters and plant biomass, and crop ripening is achieved 12-20 days earlier compared to the control options. The use of biopreparation in agriculture allows to improve the content of the organic component of the soil, thereby contributing to the restoration of the ecological balance of the soil.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292171"},"PeriodicalIF":0.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD4+/CD8+ lymphocyte ratio as a biomarker of morbidity and severity in patients with moderate and severe COVID-19.","authors":"D Torres-Gonzales, E Colona-Vallejos, L Alzamora-Gonzales, G Tineo Pozo, E Chamorro Chirinos, C Lorenzo Quito, M Aliaga Zamudio, E Cambillo Moyano, E E Aguirre-Siancas, E Ruiz Ramirez","doi":"10.1590/1519-6984.289932","DOIUrl":"https://doi.org/10.1590/1519-6984.289932","url":null,"abstract":"<p><p>The assessment of lymphocyte populations allows the monitoring, follow-up and treatment of patients with various infectious diseases. Acute respiratory syndrome caused by coronavirus (SARS-CoV-2), can lead to a decrease in circulating lymphocytes and cause disease progression and severity. We evaluated the association of CD4+/CD8+ T lymphocyte with COVID-19 disease severity in moderate and severe patients. 136 patients with COVID-19 from the moderate group (MG) and severe group (SG) were compared with a healthy group or control group (CG). CD3+, CD4+ and CD8+ T lymphocytes were quantified by flow cytometry and correlated to CD4+/CD8+ with sex, age, markers of inflammation (ferritin, C-reactive protein and D-dimer), lung damage and oxygen saturation. CD4+ and CD8+ T lymphocytes were significantly decreased in MG and SG (p<0.0001). The CD4+/CD8+ ratio showed association in MG with age (r=0.31; p=0.056), with lung damage (r=0.4; p=0.03) and in SG with total proteins (r=0.59; p=0.04) and D-dimer (r=0.57; p=0.03). The CD4+/CD8+ ratio is associated with disease severity, significantly affecting the cellular immune response.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e289932"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biometric and biochemical characterization for potential uses of Euterpe edulis (Martius) seeds.","authors":"L H G Mengarda, G B Canal, R A Braz, A Ferreira, M F S Ferreira","doi":"10.1590/1519-6984.289553","DOIUrl":"https://doi.org/10.1590/1519-6984.289553","url":null,"abstract":"<p><p>Sustainable use of Euterpe edulis for fruit pulp production is an alternative to illegal cutting of the plant. The use of its seeds, a by-product of pulp extraction, may add value to fruit production. However, characterization of the seeds is necessary to guide this use. This study characterized the fruit and seeds of E. edulis in a pulp production management area in the state of Espírito Santo, Brazil. The fruit was evaluated regarding size, volume, and color and was then pulped, quantifying the pulp yield, which was a mean of 21%. The seeds mostly consisted of crude fiber (58.9%) and lipids (18.1%). Low soluble (2.11%) and insoluble (11.15%) lignin content and high energy potential (16.25 MJ kg-1) were observed. From initial biochemical sampling, the composition of the seeds was determined by near infrared (NIR) spectroscopy for all the plants. The sugar, starch, lipid, and protein contents was more variable than the biometric profile (diameter, weight, and volume). The largest variation was observed for soluble sugar content, whereas the crude fiber content of the seeds showed little variation. The high content of fiber, as well as carbohydrates and lipids, suggests that juçara seeds can be used for producing meal for human and animal consumption. More studies are recommended on the use of oil from the seeds. The physical hardness of the seeds, the difficulty of grinding them, and their low lignin content and high energy potential indicate the use of intact seeds as biochar.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e289553"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a digital health application to support tuberculosis treatment adherence.","authors":"C Andrade-Sales, A P Simões-Castro, P A Mendonça-Cavalcante, M P Moreira-Sena, G A Tavares-Cohén, B C Brito-Alves, U G El-Awar, K Y Pacheco-Lima, C H M Azevedo-Ribeiro, J L Fernandes-Vieira, L W Pereira-Sena","doi":"10.1590/1519-6984.296271","DOIUrl":"https://doi.org/10.1590/1519-6984.296271","url":null,"abstract":"<p><p>Tuberculosis remains a serious public health challenge in Brazil, particularly among socially vulnerable populations. This study presents the development and content validation of\"Controlling the TB\", a digital prototype designed to support treatment adherence among patients undergoing tuberculosis therapy within the Brazilian Unified Health System. The tool was developed based on the Theory of Planned Behavior and User-Centered Design principles, comprising four modules: sociodemographic data, disease history, clinical guidelines, and treatment adherence. Content validation was conducted by expert professionals using a structured instrument and descriptive analysis. All modules met the minimum acceptability criteria, with notable highlights for applicability, accessible language, and clinical coherence. The results demonstrate the prototype's potential as a supportive technology for continuity of care, with national scalability and international adaptability in settings facing similar challenges in treatment adherence and digital monitoring for neglected diseases.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e296271"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contracaecum sp. larvae (Nematoda: Anisakidae) parasitizing Astyanax lacustris (Lütken, 1875) (Characiformes, Characidae) and Geophagus brasiliensis (Quoy & Gaimard, 1824) (Cichliformes, Cichlidae) in the Pardo River, São Paulo State, southeast Brazil.","authors":"H L Rocha, J C C Aguiar, R B Narciso, C Ferreira-Silva, D H M D Vieira, R J Silva","doi":"10.1590/1519-6984.290054","DOIUrl":"https://doi.org/10.1590/1519-6984.290054","url":null,"abstract":"<p><p>Astyanax lacustris (n = 26) and Geophagus brasiliensis (n = 28) were collected from two sampling sites along the Pardo River, in Botucatu, São Paulo, Brazil, and examined for parasites. Nematodes were found encysted in the intestinal serosa, heart, and fatty tissue. Morphological analysis identified the helminths as L3 larvae of Contracaecum, which was further confirmed by molecular analyses using cytochrome c oxidase subunit 2 (COX II) and large subunit ribosomal RNA (28S) genes. Phylogenetic inferences placed these larvae within a well-supported clade of Neotropical and Panamanian Contracaecum, containing sequences of Contracaecum jorgei (Argentina and Colombia) and other sequences identified as Contracaecum sp. and Contracaecum multipapillatum (Mexico, Costa Rica and Guatemala). Molecular distance analyses suggest that this Neotropical/Panamanian lineage of Contracaecum represents a single species, highlighting the fact that C. multipapillatum is currently non-monophyletic, with divergent lineages distributed across the Nearctic, Palearctic, Oriental, and Neotropical regions. Haplotype analysis supports this hypothesis with evidence of recent speciation events or ongoing gene flow within these Neotropical/Panamanian Contracaecum lineages. This new record of Contracaecum sp. larvae infecting fish in the Pardo River, Brazil, provides valuable insight into the phylogeny of this genus, which is suspected to have zoonotic potential, though no documented human infections have been reported in the Americas.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e290054"},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}