L S S Ribeiro, D R Xavier, T D S Rosa, A A Macêdo, D L S Ribeiro, F S Paz, E M C Silva, A I L Ribeiro, J R S Torres-Júnior, R B Viana, L Tchaicka, A V Carvalho-Neta
{"title":"Characterization and transcription of non-classical class I major histocompatibility complex (MHC) genes in buffaloes.","authors":"L S S Ribeiro, D R Xavier, T D S Rosa, A A Macêdo, D L S Ribeiro, F S Paz, E M C Silva, A I L Ribeiro, J R S Torres-Júnior, R B Viana, L Tchaicka, A V Carvalho-Neta","doi":"10.1590/1519-6984.281304","DOIUrl":"https://doi.org/10.1590/1519-6984.281304","url":null,"abstract":"<p><p>The objective of the present study was to characterize non-classical class I major histocompatibility complex (MHC) genes in buffaloes and evaluate the expression of these genes in different tissue components of the placenta of buffaloes during pregnancy and in trophoblastic cells after stimulation using lipopolysaccharide (LPS). To do this, DNA was extracted from the blood of buffaloes and was subjected to PCR testing and sequencing of the genes NC3 and MICB. The RNA extracted from the placentome and intercotyledonary region of buffaloes in their first (n = 6), second (n = 6) and third (n = 6) trimesters of gestation was subjected to real-time PCR. Explants were created using the chorioallantoic membrane and two experimental groups were established: control and stimulated with LPS for four hours to evaluate the gene expression profile. Analysis on the sequences obtained showed that the genes NC3 and MICB of buffaloes were homologous with those of cattle, with high similarity in the analysis on the sequence variation pattern. The gene expression analysis showed that the genes assessed were transcribed at stages and in placental tissue that differed from what was seen in cattle. The transcription of these genes varied in the tissues studied, with greater transcription of MICB in the intercotyledonary region over the first third of gestation, while the genes studied in the placentome presented low rates of transcription. The trophoblastic cells of the chorioallantoic membrane stimulated with LPS for six hours did not present non-classic MFC-I transcription alterations. The present study therefore provides additional knowledge regarding the immune regulation of placental tissues of buffaloes.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e281304"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S P B Franco, J A Silva, I C Barreto, S P M Costa, A L T F Borges, T G Nascimento, J C Silva, A F Santos, L A Rolim, J R G S Almeida
{"title":"Phytochemical constitution and pharmacological effects of Genipa americana L. (Rubiaceae): a review.","authors":"S P B Franco, J A Silva, I C Barreto, S P M Costa, A L T F Borges, T G Nascimento, J C Silva, A F Santos, L A Rolim, J R G S Almeida","doi":"10.1590/1519-6984.286031","DOIUrl":"https://doi.org/10.1590/1519-6984.286031","url":null,"abstract":"<p><p>Genipa americana L., popularly known as \"jenipapo\", is a species easily found, especially in northeastern Brazil. It is remarkable for having iridoids in its composition. Popular for its blue pigment which has been used to manufacture paints and dyes. Furthermore, this plant is utilized for medicinal purposes in addressing a variety of illnesses. This review provide a comprehensive picture of the phytochemical and biological activities characteristics of Genipa americana L. deal with its ethnomedicinal use and botany based on literature reports. These researches pointed out a range of secondary metabolites with iridoids being the most prevalent and abundant. Until now, the biological tests have shown important pharmacological activities, especially the antioxidant, insecticide and antibacterial activities. Besides presenting itself in a very promising way in other areas such as food and cosmetics industry, it is also used in popular medicine to treat several diseases having this potential use disseminated in its various parts. Finally, for a better analysis and validation of its health benefits and properties, extensive research is needed, including clinical trials. The information gathered and approached in this paper might support the planning and discussion of future studies on the topic.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e286031"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R F Oliveira, L O Vieira, D S Campos, A F G Bezerra, M S P Coelho, C T S Filgueira, B N Lima, F P Ottoni
{"title":"Distribution of Boulengerella cuvieri and B. lucius (Characiformes, Ctenoluciidae) on eastern Brazilian Amazon: updating records and discussing conservation status.","authors":"R F Oliveira, L O Vieira, D S Campos, A F G Bezerra, M S P Coelho, C T S Filgueira, B N Lima, F P Ottoni","doi":"10.1590/1519-6984.289048","DOIUrl":"https://doi.org/10.1590/1519-6984.289048","url":null,"abstract":"<p><p>Through field sampling and examination of scientific collections, this study provides new records and confirms the occurrence of Boulengerella cuvieri and B. lucius in the eastern Brazilian Amazon, northern Brazil. The occurrence of Boulengerella lucius for the Guamá River basin is here confirmed, specifically on its upper portion. The occurence of Boulengerella cuvieri is confirmed for: Guamá River basin, Pará state; Gurupi River basin, on the border between Pará and Maranhão states; and the Turiaçu River basin, in the eastern part of Maranhão state. Additionally, photographs and updated data on the distribution, conservation, taxonomy, and morphology of these two species are provided here. The results emphasize the importance of continuous database curation, especially regarding regions that are underrepresented in the scientific literature. Regarding to the species conservation status, a review and improvement of the assessment criteria is here proposed and applied, revealing a scenario worse than previously conceived.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e289048"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R A Pinheiro, M J S Cabral, L F Silva, V T S Aguilar, O R Santos, E V H Marques, S C O Giordani, C B Santos, J C Zanuncio, M A Soares
{"title":"Megalopyge lanata (Stoll, 1780) (Lepidoptera: Megalopygidae): first record of injury to Byrsonima crassifolia (L.) Kunth (Malpighiaceae) in Brazil.","authors":"R A Pinheiro, M J S Cabral, L F Silva, V T S Aguilar, O R Santos, E V H Marques, S C O Giordani, C B Santos, J C Zanuncio, M A Soares","doi":"10.1590/1519-6984.289666","DOIUrl":"https://doi.org/10.1590/1519-6984.289666","url":null,"abstract":"","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e289666"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro detection of antimicrobial impact, organic acids production and phytochemical screening of Musa acuminata wastes after treatment with fungi.","authors":"A A I Mekawy, A H Alqhtani, A Ali, M Yosri","doi":"10.1590/1519-6984.288774","DOIUrl":"https://doi.org/10.1590/1519-6984.288774","url":null,"abstract":"<p><p>The current study was performed to evaluate the bioactivity of banana wastes against some bacterial and fungal isolates, organic acids production, antioxidant and to screen the phytochemicals were present in Musa acuminate (M. acuminate) wastes. The waste was processed by some microbial species. The organic acids were evaluated in the yield using HPLC. Antioxidant impact of the yield was tested by DPPH assay. Qualitative analysis for phytochemicals were done after each treatment. M. acuminate (Banana) extracts showed moderate effect against the growth of tested microorganisms while this effect became much better after treated with fungal species (Fusarium moniliform; Rhizopus stolonifer; Aspergillus parasiticus; Alternaria alternate; Penicillium expansum and Aspergillus flavus). The MIC ranging from 6.25 to 100 mg/ml, MBC and MFC ranging from 3.125 to 250 mg/ml. Citric acid was the most produced organic acid obtained from banana wastes and highly increased after treatment with fungal isolates used. The antioxidant potential of M. acuminata leaves and pseudostem wastes enhanced after treatment with fungal extracts relative to butylated hydroxytoluene (BHT). Phytochemical screening confirmed the presence of active compounds like glycosides, tannins, saponnins, phenols, steroids and flavonoids in the M. acuminate extracts. The treated banana wastes using fungi could be used as a source of in citric acid production, antioxidant source and other chemical production to be applied in many applications.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e288774"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K Raziq, S Q A Shah, M Tariq, S Badshah, U Riaz, E Ibáñez-Arancibia, Patricio R De Los Ríos-Escalante, M M Jalees, F Badshah, F Badshah
{"title":"Prevalence and risk factors of hepatitis B and C in Bahawalpur: a hospital-based study.","authors":"K Raziq, S Q A Shah, M Tariq, S Badshah, U Riaz, E Ibáñez-Arancibia, Patricio R De Los Ríos-Escalante, M M Jalees, F Badshah, F Badshah","doi":"10.1590/1519-6984.288357","DOIUrl":"https://doi.org/10.1590/1519-6984.288357","url":null,"abstract":"<p><p>Hepatitis causes inflammation of the liver. The World Health Organization set a goal to eliminate viral hepatitis by 2030 due to an increase in the number of deaths from liver cirrhosis and hepatocellular carcinoma. The present study aimed to determine the prevalence of hepatitis B and C among the general population of Bahawalpur, along with various recognized risk factors. Samples were collected from December 2021 to May 2022. In total, 263 blood samples were collected from the patients who visited Bahawalpur Victoria Hospital, Bahawalpur Pakistan. A rapid diagnostic test (ICT) was used to diagnose Hepatitis B surface antigen and anti-HCV, followed by an enzyme-linked immunosorbent assay (ELISA). The data were analyzed using IBM SPSS version 22. In total, 263 samples were tested; 5.3% (14/263) tested positive for HBV, 25.4% (67/263) tested positive for HCV, and 0.76% (2/263) were co-infected. The study found a significant association between illiteracy 24% (63/263) and literacy 7.6% (20/263) (P = 0.001), age (31-40 years) 11% (29/263) (P = 0.027), hospitalization 38% (100/263) versus non-hospitalization 62% (163/263) (P = 0.008), and patients who had received any dental treatment 20.9% (55/263) versus those who had not 79.1% (208/263) (P = 0.057). According to the study, people who frequently visited hospitals received no medical care and were unaware of the causes of disease transmission, made them more susceptible to infection. The study will help formulate policies and adopt precautionary measures to control the increasing incidence of hepatitis B and C in Pakistan.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e288357"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Moreira, A C O Meirelles, V L Carvalho, P S Rêgo, J Araripe
{"title":"Molecular evidence supporting a recurrent stranding event in West Indian manatees.","authors":"S Moreira, A C O Meirelles, V L Carvalho, P S Rêgo, J Araripe","doi":"10.1590/1519-6984.285381","DOIUrl":"https://doi.org/10.1590/1519-6984.285381","url":null,"abstract":"","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e285381"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S K Ferreira-Silva, R J R Alexandre, S A Pena, M D L Lucena, T B Vieira, F B R Gomes
{"title":"Associations between morphological attributes and food resources in anurans from the Middle Xingu region, Brazil.","authors":"S K Ferreira-Silva, R J R Alexandre, S A Pena, M D L Lucena, T B Vieira, F B R Gomes","doi":"10.1590/1519-6984.274592","DOIUrl":"https://doi.org/10.1590/1519-6984.274592","url":null,"abstract":"<p><p>Considering the hypothesis that there is a relationship between morphology and the choice or feeding habit in anurans, our objective was to identify the food items present in the anurans' diet; verify the existence of a pattern in the diet of the anurans collected; and show the relationship between diet and species morphology. Sampling was carried out using an active search methodology carried out by three people, lasting approximately three hours per unit. For that work, only species that had an abundance equal to or greater than five individuals were considered. We identified dietary patterns in taxonomic groups and tested their significance, in addition to groupings by family, genus and species and the relationship between morphology and diet. 148 individuals were collected, belonging to 41 anuran species, 16 genera and nine families, 44 items were observed, highlighting the Formicidae family and Tadpoles, with shrimp and crab being the least frequently found items. We concluded that there was a relationship between morphology and diet, in addition to the formation of a guild of insectivorous anurans, indicating a greater frequency of Hymenoptera as a food resource, mainly composed of the genus Rhinella.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e274592"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F V Diniz, J E Scherwinski-Pereira, F H S Costa, C M Carvalho
{"title":"Effects on plant physiology in response to inoculation of growth-promoting bacteria: systematic review.","authors":"F V Diniz, J E Scherwinski-Pereira, F H S Costa, C M Carvalho","doi":"10.1590/1519-6984.287279","DOIUrl":"https://doi.org/10.1590/1519-6984.287279","url":null,"abstract":"<p><p>Changes in physiological mechanisms resulting from the association of plant growth-promoting bacteria as well as the responses generated to stressful factors are of interest for sustainable agriculture. Based on this, the objective of this study was to gather insights from recent years (2012-2022) on the impacts on plant physiology of the use of inoculants from plant growth-promoting bacteria. To do this, the search for articles was done in three different databases, Science Direct, Springer Nature and Google Scholar, using the following descriptors: plant growth promoting bacteria, plant hormones, biological control, photosynthesis and abiotic stress. After selection, the included articles were systematized in the Excel program. Pearson Correlation and Principal Component Analysis were used for comparative analysis of physiological variables. 81 articles were included in the review, where a beneficial association was observed in 45 plant species distributed in 13 Orders and 13 Families, with emphasis on the Families Poaceae, Fabaceae, Solanaceae and Brassicaceae. 47 genera and 98 bacterial species were verified, where Bacillus and Pseudomonas represented 52% of the verified strains, with emphasis on Bacillus subtilis and Pseudomonas fluorescens. The main applications were growth promotion, productivity, control of biotic stress and abiotic stress. Positive regulation of photosynthesis was observed, modulating the gene expression of photosynthetic apparatus proteins, pigments, antioxidant production, increased hormonal and nutritional production, osmolyte content, antimicrobial production and decreased lipid peroxidation. Based on this review, it was possible to understand the multifaceted role of plant growth-promoting bacteria in contributing to the better direction of technology in agriculture.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e287279"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}