Brazilian Journal of Biology最新文献

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Stimulation of the production of proteases from Penicillium verrucosum isolated from Egyptian soil. 从埃及土壤中分离的疣状青霉对蛋白酶产生的刺激作用。
Brazilian Journal of Biology Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.296244
A A Garziz, M Helal, A M Younis, M A El-Badry, M Yosri
{"title":"Stimulation of the production of proteases from Penicillium verrucosum isolated from Egyptian soil.","authors":"A A Garziz, M Helal, A M Younis, M A El-Badry, M Yosri","doi":"10.1590/1519-6984.296244","DOIUrl":"10.1590/1519-6984.296244","url":null,"abstract":"<p><p>The uses of proteolytic enzymes in manufacturing procedures are numerous. Researchers are investigating a number of strategies to find, rework, or artificially produce enzymes with improved suitability for manufacturing processes in light of the growing needs and uses. Alkaline protease production was assessed in fungal strains that were obtained from soil using the serial dilution technique. Using molecular techniques, the isolate with the greatest potential for producing enzymes was identified. Gel filtering was used to purify the enzyme. In order to enhance fugus ability to generate protease under various growth conditions, this investigation explains how to optimize a culture medium. The fungus with the highest probability was identified as Penicillium verrucosum with accession number PV529631. The purified homodimer protein for purified protease was assessed to have two peaks at 42.5 kDa and 83.7 kDa, consequently upon screening at 280 nm. The optimal culture conditions have been detected upon culture for the isolated fungus at pH =9, temperature = 35 °C, using sucrose as a carbon source, insertion of peptone as a nitrogen and using Soy cake as waste material for culture conditions. The results of the research demonstrate the P. verrucosum strain's capacity to produce the protease enzyme. To fully use the possibilities of these substrates and promote the creation of superior and effective hydrolytic enzymes, it is imperative to keep investigating and refining these approaches.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e296244"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The impact of the COVID-19 pandemic on home gardening practices in Irbid District, Jordan. 2019冠状病毒病大流行对约旦伊尔比德地区家庭园艺实践的影响
Brazilian Journal of Biology Pub Date : 2025-08-29 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.295378
R W Tahtamouni, R A Shibli, T S Al-Qudah, S Saifan, L S Younes
{"title":"The impact of the COVID-19 pandemic on home gardening practices in Irbid District, Jordan.","authors":"R W Tahtamouni, R A Shibli, T S Al-Qudah, S Saifan, L S Younes","doi":"10.1590/1519-6984.295378","DOIUrl":"10.1590/1519-6984.295378","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Home gardening has been instrumental in enhancing the self-sufficiency initiatives of families across the globe. Numerous researched emphasized the positive impacts of practicing home gardening throughout COVID-19. In Jordan, few studies were conducted to examine the influence of COVID-19 on various facets of life, including employment and the livelihoods of Jordanians, but no research had discussed the pandemic impacts on home gardening. To address this research gap, a quantitative survey was conducted to assess the attitudinal, behavioral, and emotional responses related to home gardening among individuals residing in the Irbid District, both prior to and during the COVID-19 pandemic. The survey instrument comprised 11 statements, with responses measured on a 5-point Likert scale, ranging from 1 (very low extent) to 5 (very high extent). A total of 374 Jordanian participants from the Irbid District completed the questionnaire. Furthermore, the study aimed to ascertain whether demographic variables, specifically age and educational background, significantly influenced these trends during the pandemic. Analysis of the collected data revealed several key findings, as participants reported a moderate level of importance attributed to home gardening during the COVID-19 pandemic, a notable increase from the low importance perceived prior to the pandemic. Also, a significant increase in the time dedicated to home gardening activities was observed during the pandemic compared to pre-pandemic periods. Moreover, the respondents indicated that home gardening during the pandemic was associated with increased happiness and a substantial reduction in stress levels, while the perceived contribution of home gardening to household food security rose to a moderate extent during the pandemic, contrasting with a pre-pandemic rating of low. Also, a shift in preference towards cultivating edible plants over ornamental varieties was evident during the COVID-19 period. According to the results, a substantial proportion of participants (49.2%) utilized internet and social media platforms for acquiring home gardening information, while reading books was the least preferred method. Also, statistically significant differences were identified in home gardening trends during and prior to the COVID-19 pandemic concerning age and academic qualifications. These differences were more pronounced among the youngest age cohort (18-30 years) and individuals with lower educational attainment. The findings of this study indicate that the COVID-19 pandemic significantly influenced home gardening practices in the Irbid district, demonstrating a positive shift in citizen perceptions and engagement. The increased importance and engagement in home gardening during the pandemic highlight its adaptive capacity in supporting both individual well-being and household food security during a public health crisis. Future research is recommended to investigate the long-term sustainability of","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295378"},"PeriodicalIF":0.0,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expansion of the geographic distribution and morphological aspects of Braga patagonica (Isopoda: Cymothoidae) to Eastern Amazon, Brazil. 巴西东亚马逊地区巴塔哥尼加的地理分布和形态特征的扩展(等足目:银足科)。
Brazilian Journal of Biology Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.293786
I C S Oliveira, L Cardoso, I C M Lima, N J Pereira, M M Santos, M J Machado, G T Jerônimo, D M S Santos, E C Fraga
{"title":"Expansion of the geographic distribution and morphological aspects of Braga patagonica (Isopoda: Cymothoidae) to Eastern Amazon, Brazil.","authors":"I C S Oliveira, L Cardoso, I C M Lima, N J Pereira, M M Santos, M J Machado, G T Jerônimo, D M S Santos, E C Fraga","doi":"10.1590/1519-6984.293786","DOIUrl":"10.1590/1519-6984.293786","url":null,"abstract":"<p><p>The Amazon rainforest and its rivers host a vast biodiversity, including many unique yet undiscovered species, particularly among Amazonian fish. These organisms can be susceptible to ectoparasites, such as isopod crustaceans. This study describes the presence of the isopod Braga patagonica parasitizing Hoplias malabaricus in the state of Maranhão, located in the eastern Amazon region of Brazil. Isopod specimens were collected from the fish bodies in two sampling periods conducted between 2021 and 2022 and identified using dichotomous keys based on morphological analysis and measurements of the parasites. Furthermore, parasitological indices were calculated, including prevalence rate (P%), mean intensity (MI), and parasite abundance (PA). A total of 25 parasites, including females and males, were found fixed in the gill cavity, on the integument, and near the pectoral and pelvic fins. During the first collection period, the hosts exhibited a prevalence rate of 100%, with MI and PA of 1.0 ± 0.00. In contrast, during the second collection period, the prevalence rate decreased to 66.67%, with MI of 1.0 ± 0.04 and PA of 0.64 ± 0.49. Females were larger, measuring approximately 2.09 cm in length and 1.09 cm in width, while males reached 1.96 cm in length and 0.86 cm in width, and displayed a lighter coloration compared to the females in both collections. This study establishes a new geographical occurrence of Braga patagonica in the state of Maranhão, in Northeast Brazil, providing a comprehensive analysis of the morphological characteristics of this isopod. The results provide valuable insights into the distribution of the Cymothoidae family in the Eastern Amazon.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293786"},"PeriodicalIF":0.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gas exchange and bioactivity in yellow passion fruit seedlings cv. Rubi do Cerrado under organic substrates and water levels. 百香果幼苗气体交换及生物活性研究。Rubi do Cerrado在有机基质和水位下。
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.292697
L A P Silva-Filho, E F Mesquita, C S Sousa, J P C Diniz, S N Silva
{"title":"Gas exchange and bioactivity in yellow passion fruit seedlings cv. Rubi do Cerrado under organic substrates and water levels.","authors":"L A P Silva-Filho, E F Mesquita, C S Sousa, J P C Diniz, S N Silva","doi":"10.1590/1519-6984.292697","DOIUrl":"https://doi.org/10.1590/1519-6984.292697","url":null,"abstract":"<p><strong>Background: </strong>Fertilization and water supply are indispensable factors in the initial growth of passion fruit and, consequently, in its production in the field. The aim of this study was to evaluate the influence of organic substrates and water levels on gas exchange and bioactive compounds in passion fruit seedlings (cv. BRS Rubi do Cerrado). The treatments consisted of a 5 × 2 factorial arrangement, comprising five organic substrates: S1= 100% soil, S2= 75% soil and 25% cattle manure, S3= 75% soil and 25% goat manure, S4= 75% soil and 25% worm humus, S5= 75% soil and 25% organic compost and two water levels: L1 50% and L2 100% of water available in the substrate, with six replicates. Gas exchange was analyzed: stomatal conductance - gs, transpiration - E, CO2 assimilation rate - A and internal CO2 concentration - Ci. From the data obtained, the instantaneous water use efficiency - EiUA and the instantaneous carboxylation efficiency - EiCi were estimated, as well as the bioactive compounds: total carotenoids, chlorophyll a and b, phenolic compounds and proline. When the seedlings were irrigated with 100% available water, they showed higher stomatal conductance, transpiration, CO2 assimilation rate and internal CO2 concentration, resulting in greater carboxylation. Seedlings grown in substrates containing cattle manure and worm humus showed better responses to water deficit. There was an influence of the different substrates and water levels on the gas exchange and bioactive compounds of passion fruit seedlings cv. Rubi do Cerrado.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292697"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical, laboratory and epidemiological aspects of Cryptosporidium parvum and Cryptosporidium hominis infection: a integrative review. 细小隐孢子虫和人隐孢子虫感染的临床、实验室和流行病学方面:综合综述。
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.283306
R L M Mendonça, M S Miranda, A C Guimarães, A P Queiroga, H F M Câmara, P H L T S Anjos, J V Ferro, H B G Messias, T L G Q Maranhão, A C S Omena, A C Silva, C J Santos Júnior, B C Omena, M G S Cavalcanti, T J M Rocha, G C Ferreira Júnior
{"title":"Clinical, laboratory and epidemiological aspects of Cryptosporidium parvum and Cryptosporidium hominis infection: a integrative review.","authors":"R L M Mendonça, M S Miranda, A C Guimarães, A P Queiroga, H F M Câmara, P H L T S Anjos, J V Ferro, H B G Messias, T L G Q Maranhão, A C S Omena, A C Silva, C J Santos Júnior, B C Omena, M G S Cavalcanti, T J M Rocha, G C Ferreira Júnior","doi":"10.1590/1519-6984.283306","DOIUrl":"https://doi.org/10.1590/1519-6984.283306","url":null,"abstract":"<p><strong>Background: </strong>Cryptosporidium parvum and Cryptosporidium hominis infection causes cryptosporidiosis, which in humans can result in watery diarrhea that is usually self-limited in immunocompetent individuals, but can be chronic and fatal in immunocompromised individuals. To understand and expose current clinical, laboratory and epidemiological data, as well as characteristics of cryptosporidiosis worldwide. This is a literature review by the integrative review method, qualitative and exploratory through the publications selected in the databases: BVS, ScieLO, MEDLINE and LILACS. Inclusion criteria were: publications from the last 6 years and articles in Portuguese, English and Spanish. Exclusion criteria were: research not evidenced in humans and duplicate articles, as well as articles that were not available in full and free of charge. The variables analyzed were cases by regions of the world, transmission mechanisms and symptoms. In this study, 40 articles were submitted. Regarding transmission, the most commonly reported route was the anthroponotic route, being mentioned in 40% of them (16); the zoonotic route was also prominent, being mentioned in 22.5% (9) of the articles. As for the symptoms, diarrhea was the most present, being mentioned in 82.5% (33 articles), other symptoms mentioned were headache 12.5% (5), joint pain 5% (2) and eye pain 5% (2). With regard to laboratory diagnosis, the most commonly used method was polymerase chain reaction (PCR) 75% (30) and modified Ziehl Neelsen staining 22.5% (9). Therefore, it is noticeable that the main complication of cryptosporidiosis is gastroenteritis, whose manifestations range from diarrhea to abdominal cramps. This parasite is widely distributed throughout the world, mainly in developing and underdeveloped countries, transmitted by the oral-fecal route. In addition, it is notable that PCR was the most commonly used laboratory method for diagnosis, which shows that the disease can be easily detected once the symptoms have manifested themselves. In this sense, knowledge about the parasite is necessary for prevention and thus avoid major complications.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e283306"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro digestibility evaluation of Axonopus Scoparius under Ecuadorian conditions: baseline results. 厄瓜多尔条件下东棘爪虫体外消化率评价:基线结果。
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.296065
M P P Orozco, L S A Alemán, M M F Ramos, E F Lozada
{"title":"In vitro digestibility evaluation of Axonopus Scoparius under Ecuadorian conditions: baseline results.","authors":"M P P Orozco, L S A Alemán, M M F Ramos, E F Lozada","doi":"10.1590/1519-6984.296065","DOIUrl":"https://doi.org/10.1590/1519-6984.296065","url":null,"abstract":"<p><strong>Background: </strong>Axonopus scoparius, a native forage grass species of the Ecuadorian Amazon, holds significant potential as a sustainable feed resource for ruminant production systems, yet its nutritional characteristics remain insufficiently studied. This research aimed to evaluate the in vitro dry matter digestibility (IVDMD) of A. scoparius collected from six ecological zones with altitudes ranging from 280 to 945 meters above sea level. The study assessed digestibility across three incubation periods (24, 48, and 72 hours) using the established Tilley and Terry two-stage technique to simulate ruminal fermentation and post-ruminal digestion. Samples were systematically collected to represent diverse altitudinal and ecological conditions, providing insights into how environmental factors influence forage quality. Statistical analyses included analysis of variance (ANOVA), Tukey's multiple comparison tests, and regression modeling to examine the effects of incubation time and altitude on digestibility outcomes. Results demonstrated a significant increase (P < 0.01) in IVDMD with longer incubation, with mean values rising from 22.46% at 24 hours, to 30.74% at 48 hours, and reaching 37.81% after 72 hours of fermentation. Altitude was a significant factor affecting digestibility (P = 0.004); notably, samples from mid-altitude zones (600-750 m.a.s.l.) showed the highest digestibility levels, up to 42.4%, whereas lower altitude zones exhibited values as low as 26.8%. Regression analysis revealed a positive correlation between incubation time and digestibility at 72 hours (r = 0.5289, R2 = 0.2797, p = 0.0079), highlighting the importance of extended fermentation periods to capture the full nutritional potential of this grass species. While A. scoparius exhibits moderate digestibility compared to improved tropical forages, its natural adaptability to poor soils, resilience to overgrazing, and minimal input requirements position it as a valuable forage option for extensive tropical livestock systems. These preliminary findings advocate for the integration of A. scoparius into forage diversification strategies and suggest further research focusing on agronomic practices, supplementation, and genetic improvement to enhance its nutritive value.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e296065"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective application of malathion: effect on boll weevil, non-target insects and natural enemies. 马拉硫磷的选择性施用:对棉铃象鼻虫、非目标昆虫和天敌的影响。
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.292174
S F Camelo, C A D Silva, J C Zanuncio
{"title":"Selective application of malathion: effect on boll weevil, non-target insects and natural enemies.","authors":"S F Camelo, C A D Silva, J C Zanuncio","doi":"10.1590/1519-6984.292174","DOIUrl":"https://doi.org/10.1590/1519-6984.292174","url":null,"abstract":"<p><strong>Background: </strong>Non-selective chemical insecticides are the main method to manage the major insect pest of cotton in the Americas, the boll weevil Anthonomus grandis (Boheman, 1843) (Coleoptera: Curculionidae). The objective was to evaluate the effect of selective application of malathion on the boll weevil and non-target insects and natural enemies. The experimental design was in randomized blocks with the treatments control, without spraying (T1) and with all cotton plants in the rows sprayed, sequentially, after the appearance of cotton squares or all (T3) or alternated (T4), respectively, when the number of them with cotton squares with oviposition punctures by the boll weevil reached 10%. Spraying was done with malathion (1,000 g.i.a. L-1). The insecticide malathion sprayed on alternate rows of cotton is as effective in controlling the boll weevil as when sprayed on all rows. However, this insecticide applied to alternate rows controls aphid populations because it is ecologically selective to the parasitoid B. vulgaris and the predators, Coccinellidae, Syrphidae, and Araneae.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292174"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soil morphological and chemical characteristics in the habitats of the rare, endemic plant Spiraeanthus schrenkianus in Karatau Mountains, Kazakhstan. 哈萨克斯坦卡拉托山珍稀特有植物schrenkianus螺旋藻生境土壤形态与化学特征
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.295015
A Muratbayeva, А Nurmahanova, K Kulymbet, A Myltykbayeva, A Akhmetova, B Abdullayeva, S Atabayeva, G Baitasheva, E Kyrbassova, Z Omarova
{"title":"Soil morphological and chemical characteristics in the habitats of the rare, endemic plant Spiraeanthus schrenkianus in Karatau Mountains, Kazakhstan.","authors":"A Muratbayeva, А Nurmahanova, K Kulymbet, A Myltykbayeva, A Akhmetova, B Abdullayeva, S Atabayeva, G Baitasheva, E Kyrbassova, Z Omarova","doi":"10.1590/1519-6984.295015","DOIUrl":"https://doi.org/10.1590/1519-6984.295015","url":null,"abstract":"<p><strong>Background: </strong>The article presents the results of the study of ecological characteristics of soils of three populations on the territory of Karatau. The populations were located in different geographical points with differences in soil types, depth of horizons and content of chemical elements. The study evaluated the main soil parameters including humus content, pH, CO2, macro- and microelements, and salinity level. It was found that population-1 (mountain gray-brown soils) was characterized by the highest content of humus and mobile nitrogen, indicating better availability of nutrients to plants. population-2 (mountain southern serozems) has higher potassium content, which is favourable for plant growth. population-3 (mountain gray-brown soils) showed the lowest content of mobile phosphorus and copper compared to other populations. Analysis of heavy metal concentrations showed that zinc exceeded the maximum permissible concentration only in population-2. At the same time, soil salinization at all three sites remains at a low level and does not pose a threat to the ecosystem. These findings are ecologically significant as they reveal the critical role of soil fertility and contamination in supporting the distribution and survival of the rare endemic species Spiraeanthus schrenkianus. These results provide new information for assessing the fertility and sustainability of land resources.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295015"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of irrigation with wastewater on soil fertility and growth of forage palm (Opuntia tuna) and sabiá (Mimosa caesalpiniifolia Benth) under degraded soil in semi-arid Brazil. 巴西半干旱退化土壤下废水灌溉对饲用棕榈和含羞草<e:1>土壤肥力和生长的影响
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.291665
D A Meireles, K A A Carneiro, F A Silva, V S Fraga, B O Dias, D S Batista, R V Santos, A F L Lima, M C C Campos
{"title":"Effects of irrigation with wastewater on soil fertility and growth of forage palm (Opuntia tuna) and sabiá (Mimosa caesalpiniifolia Benth) under degraded soil in semi-arid Brazil.","authors":"D A Meireles, K A A Carneiro, F A Silva, V S Fraga, B O Dias, D S Batista, R V Santos, A F L Lima, M C C Campos","doi":"10.1590/1519-6984.291665","DOIUrl":"https://doi.org/10.1590/1519-6984.291665","url":null,"abstract":"<p><strong>Background: </strong>Water scarcity is growing every day, especially in semi-arid regions. Consequently, the amount of groundwater used in agriculture is strictly limited, and it has become necessary to explore alternative water sources. This study aimed to evaluate the potential of wastewater in restoring the fertility of degraded soil and in the vegetative growth of forage palm (Opuntia tuna) and sabiá (Mimosa caesalpiniifolia Benth). The experiment was conducted in the semi-arid region of Paraíba in a randomized block design arranged in a 3x2 factorial arrangement, with three types of irrigation water: i) irrigation with a volume of 500 ml plant/week with water supply; ii) irrigation with a volume of 500 ml plant/week of domestic sewage wastewater; iii) irrigation with a volume of 1000 ml plant/week of domestic sewage wastewater/ and two plant species: i) forage palm and ii) sabiá, with 10 replications. The biometric variables of the forage palm and sabiá plants were assessed. Soil samples were taken before and after irrigation to characterize soil fertility. It was found that wastewater changed pH in water, cation exchange capacity, base saturation, organic carbon, Ntotal, and available phosphorus. The treatment with the highest level of wastewater favored the vegetative growth of the forage palm, while the sabiá plants showed no significant response.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e291665"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison between different protocols for DNA extraction for Hypostomus affinis. 拟合下口蝇DNA提取不同方法的比较。
Brazilian Journal of Biology Pub Date : 2025-08-18 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.292421
M Rodrigues-Zeferino, S Oliveira-Olimpio, P C Costa-Pierro, M Fanttini-Polese, J D Souza-Neto, M Moreira-Moulin, P Pierro-Mendonça
{"title":"Comparison between different protocols for DNA extraction for Hypostomus affinis.","authors":"M Rodrigues-Zeferino, S Oliveira-Olimpio, P C Costa-Pierro, M Fanttini-Polese, J D Souza-Neto, M Moreira-Moulin, P Pierro-Mendonça","doi":"10.1590/1519-6984.292421","DOIUrl":"https://doi.org/10.1590/1519-6984.292421","url":null,"abstract":"<p><strong>Background: </strong>The sand catfish or painted catfish (Hypostomus affinis) is a species native to Brazil and has some peculiar characteristics: a flattened body covered by bony plates and fins with spikes for fixing on rocks. Molecular studies have been carried out to better understand this and other species of fish. In this study, a comparison was made between DNA extraction methodologies to define the best extraction protocol for this species. For the study, approximately two grams of the caudal fin of twenty adult specimens of Hypostomus affinis were used for DNA extraction. For this, three extraction protocols were performed. The quantification of the genetic material was carried out in a NanoDrop 2000c Spectrophotometer, thus obtaining the DNA concentration in ng/µL and purity of each sample (A260/A280 and 230/260 ratio). Protocol I, based on Barrero, was the one that presented the best result of DNA concentration of 2846.28 ng/µL. Showing no difference from the III NaCL, which showed 2385.69 ng/µL of DNA. It is concluded that the protocol I presented the best results in terms of quantity and purity.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292421"},"PeriodicalIF":0.0,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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