Brazilian Journal of Biology最新文献_第5页

Identification of fava bean genotypes tolerant and susceptible to water deficit. 耐亏水易感蚕豆基因型的鉴定。

Brazilian Journal of Biology Pub Date : 2025-06-06 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.295367

S G Jacinto Junior, L F Pinheiro, V B Ramos, P L M Santiago, A L S L Ribeiro, E M P Lucena

{"title":"Identification of fava bean genotypes tolerant and susceptible to water deficit.","authors":"S G Jacinto Junior, L F Pinheiro, V B Ramos, P L M Santiago, A L S L Ribeiro, E M P Lucena","doi":"10.1590/1519-6984.295367","DOIUrl":"https://doi.org/10.1590/1519-6984.295367","url":null,"abstract":"Of the genus Phaseolus, fava beans are of great socio-economic importance to the Brazilian Northeast due to their nutritional profile and dryland cultivation. Legume cultivation has been threatened by environmental changes and processes of aridity and desertification around the world, especially in the semi-arid region of Brazil, which has high rainfall variability with irregular rainfall throughout the year. This research aimed to identify fava bean genotypes that are tolerant and susceptible to water deficit in seedlings. The trials were carried out at the Plant Biology Laboratory (LABIV) of the State University of Ceará (UECE), in Fortaleza-CE. Sixteen cultivars were selected and parameters related to the physiological quality of the seeds were analyzed under conditions of water stress induced by PEG 6000, with osmotic potentials of 0.0 MPa and -0.3 MPa. A survival curve was used to identify contrasting genotypes. It was observed that the germination test and germination speed index explained approximately 77% of the variability in the data. The genotypes BF 306 and BF 313 presented better performance than the other cultivars, showing tolerance to water deficit, while the genotypes BF 308, BF 302, BF 311, BF 303 and BF 293 obtained the worst values, showing their susceptibility to drought. The Screening Box method showed that genotype BF 301 was susceptible to water deficit and its survival was restricted to 25 days, indicating its greater vulnerability to water deficit in relation to other analyzed cultivars.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295367"},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of Paspalum accessions for use in poorly drained soils. 排水不良土壤中雀稗材料的选择。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.293705

L M Benez, P M Santos, A P Fávero, J C G Santos, G C Ferreira, A A F Garcia

{"title":"Selection of Paspalum accessions for use in poorly drained soils.","authors":"L M Benez, P M Santos, A P Fávero, J C G Santos, G C Ferreira, A A F Garcia","doi":"10.1590/1519-6984.293705","DOIUrl":"https://doi.org/10.1590/1519-6984.293705","url":null,"abstract":"Excess soil water can result from inadequate drainage, seasonal groundwater fluctuations, and extreme rainfall events. Diversifying forage species is a key strategy for mitigating these challenges. This study aimed to evaluate Paspalum germplasm collected from different regions of Brazil and identify accessions with potential for use in poorly drained environments. A total of twenty-three Paspalum accessions were assessed under two water regimes (flooded and non-flooded), alongside Urochloa brizantha cv. Marandu, in a randomized block design with a 24 × 2 factorial arrangement and three replications. The experiment was conducted in a greenhouse using potted plants. The evaluated traits were dry mass of leaves, stems, dead material, aerial biomass, roots, the ratio of dead material dry mass to aerial biomass, leaf area, number of tillers, and leaf elongation. Data were subjected to analysis of variance and a means comparison test at a 5% significance level, followed by biplot analysis. All genotypes were affected by flooding; however, BGP238, BGP112, and BGP410 exhibited superior performance in the assessed growth and biomass production traits compared to U. brizantha cv. Marandu under both flooded and non-flooded conditions. These genotypes can advance in plant breeding studies.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e293705"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconversion of Amazonian vegetables by Pleurotus albidus: production of fibrinolytic enzyme. 白侧耳菇对亚马逊蔬菜的生物转化：纤维蛋白溶酶的产生。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.290015

L Pimenta, E E P Barbosa, K S Araújo, A K P Brito, S C P Batista, S R Martim, W Rocha, M F S Teixeira

{"title":"Bioconversion of Amazonian vegetables by Pleurotus albidus: production of fibrinolytic enzyme.","authors":"L Pimenta, E E P Barbosa, K S Araújo, A K P Brito, S C P Batista, S R Martim, W Rocha, M F S Teixeira","doi":"10.1590/1519-6984.290015","DOIUrl":"https://doi.org/10.1590/1519-6984.290015","url":null,"abstract":"Edible mushrooms are used for a variety of applications, such as the bioconversion of agricultural products to produce fermented foods rich in bioactives of medicinal importance, such as fibrinolytic enzymes. The objective of this study was to evaluate the production of fibrinolytic proteases through the bioconversion of Amazonian vegetables by a species of edible Amazonian mushroom. Pleurotus albidus was provided by the Department of Parasitology, Federal University of Amazonas (DPUA) culture collection at the Federal University of Amazonas. Bioconversion was carried out on the following vegetables: Thorn yam (Dioscorea altissima), legume seed of black-eyed beans (Vigna unguiculata), and wheat grain (Triticum aestivum) at 25 °C. Extraction was carried out in an aqueous medium, the extracts were recovered and dialyzed. P. albidus excreted proteases in all vegetables tested. In the fibrin plaque, a translucent halo of significant value was determined in Black-Eyed Beans (77.33 mm ± 0.13). The maximum activity of the fibrinolytic enzymes synthesized was 850.2 ± 0.01 U/mL, with an increase to 1,694.7 ± 0.10 in the dialyzed extract. Thus, fibrinolytic enzymes through the bioconversion of vegetables by this edible mushroom species proved to be effective in synthesis and the method evaluated for effective purification, as well as being shown as a promising source for the production of new drugs for coagulant diseases.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e290015"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a sustainable exploitation system for stony materials in the special management zone of the Upano River, Province of Morona Santiago. Morona Santiago省Upano河特别管理区石质材料可持续开发系统的设计。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.295259

J C Farez Atiencia, C Lara-Basantes, A R Moyota Paguay, M P Feijoo Alvarez, C Narvaéz-Flores

{"title":"Design of a sustainable exploitation system for stony materials in the special management zone of the Upano River, Province of Morona Santiago.","authors":"J C Farez Atiencia, C Lara-Basantes, A R Moyota Paguay, M P Feijoo Alvarez, C Narvaéz-Flores","doi":"10.1590/1519-6984.295259","DOIUrl":"https://doi.org/10.1590/1519-6984.295259","url":null,"abstract":"This study aims to design a sustainable system for the extraction of stone materials in the Upano River Special Management Zone, incorporating technical, environmental, and social criteria to minimize impacts on a sensitive ecosystem. The exploitation of natural resources, especially in sensitive ecosystems such as river systems, must be approached with a comprehensive and multidisciplinary perspective. This theoretical framework integrates principles from environmental science, sustainable development, resource management, and community engagement to design a system that minimizes environmental degradation while promoting socio-economic benefits. The methodology involved characterizing the riverbed, identifying alluvial deposits, and assessing the natural replenishment capacity of the river. The results showed that the deposits were composed mainly of blocks, gravels (74%), sands (20%), and fines (6%), all exhibiting high mechanical quality for construction. The natural replenishment rates were estimated at 76,101 m3 every six months for zone A (upstream) and 38,867 m3 for zone B (downstream), with daily replenishment rates of 422.79 m3 and 215.89 m3, respectively. Based on these data, sustainable extraction rates were determined to align with the river's natural dynamics. Environmental mitigation strategies included sediment barriers, continuous water quality monitoring, and reforestation with native species. Additionally, topographic and bathymetric technologies were employed to delimit extraction areas, minimizing alterations to the riverbed.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295259"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new semi-synthetic carboximidamide mitigates sickness behavior in mice. 一种新的半合成碳胺减轻小鼠的疾病行为。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.290683

L C R Hilgenberg, L D R Acho, G R Batista, J P Moura-Neto, E S Lima, C C Veloso

{"title":"A new semi-synthetic carboximidamide mitigates sickness behavior in mice.","authors":"L C R Hilgenberg, L D R Acho, G R Batista, J P Moura-Neto, E S Lima, C C Veloso","doi":"10.1590/1519-6984.290683","DOIUrl":"https://doi.org/10.1590/1519-6984.290683","url":null,"abstract":"In the course of an infection, increased pro-inflammatory cytokines cause diseased individuals to develop a set of adaptive behavioral changes, collectively termed as sickness behavior. The administration of the endotoxin LPS, the main component of the outer membrane of Gram-negative bacteria, leads to activation of the immune system, inducing inflammatory process in experimental models. A new carboxymidamide (DCHA), which has potential anti-inflammatory activity, may be a promising candidate in the development of a new drug for inflammatory diseases and control of sickness behavior. This study was conducted to evaluate the effects of a novel carboxymidamide on lipopolysaccharide-induced sickness behavior in mice. The animals were submitted to open field tests and forced swimming two hours after treatment with LPS (100 µg/kg). In addition, the mRNA expression of iNOS and pro-inflammatory cytokines (IL-1β, TNF-α and IL-6) from the blood, liver and kidney of these animals was also evaluated using quantitative real-time PCR. The administration of lipopolysaccharide (LPS, 100 µg/kg, i.p.) induced behavioral changes in the mice, such as decreased locomotor and exploratory activity in the open field test and an increase in the time the animal remained floating in the forced swimming, as well as increased mRNA expression of iNOS and the pro-inflammatory cytokines IL-1β, TNF-α and IL-6. Pretreatment with the new carboxymidamide attenuated the behavioral changes induced by LPS, and significantly reduced the expression of iNOS and pro-inflammatory cytokines in mice. Thus, our experimental results demonstrated that DCHA attenuated the sickness behavior induced by LPS. These results suggest that the observed pharmacological effect is due to the in vivo anti-inflammatory action via reduction of pro-inflammatory cytokines.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e290683"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of SARS-CoV-2 variants associated with reinfection cases in Botucatu, Brazil: a comparative genomic study. 与巴西波图卡图再感染病例相关的SARS-CoV-2变异特征：一项比较基因组研究

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.292021

A M S Kubo, R A Silveira, D B S Figueiredo, L A Umezawa, K I Tasca, C M C B Fortaleza, G T Valente, R M T Grotto

{"title":"Characterization of SARS-CoV-2 variants associated with reinfection cases in Botucatu, Brazil: a comparative genomic study.","authors":"A M S Kubo, R A Silveira, D B S Figueiredo, L A Umezawa, K I Tasca, C M C B Fortaleza, G T Valente, R M T Grotto","doi":"10.1590/1519-6984.292021","DOIUrl":"https://doi.org/10.1590/1519-6984.292021","url":null,"abstract":"The global spread of SARS-CoV-2 triggered the COVID-19 pandemic, resulting in a significant number of deaths. Variants such as B.1.1.7, B.1.351, P.1, and BA.1 distinguished by spike protein mutations arose due to immune pressure and lack of repair mechanisms. These changes amplified infectivity, increasing the chances of reinfection. We retrospectively included patients who have two positive tests for SARS-CoV-2 detected by real-time reverse transcription polymerase chain reaction with an interval of 90 days, or more, between 01 March 2020 and 30 April 2022. SARS-CoV-2 whole genome sequencing was performed for both infections, and the variants were identified using the Phylogenetic Assignment of Named Global Outbreak Lineages (Pangolin) database. This study identified a total of 254 cases of reinfections, 50.8% of the cases caused by the P.1 and BA.1 variants. Compared to the state scenario, a lower detection of the B.1.617.2 variant was identified, highlighting the importance of mass vaccination in the city of Botucatu, Brazil. However, most second infections (98.8%) were identified during the BA.1 wave, showing the effect of viral adaptative mechanisms on infection dynamics even in a vaccinated population. Taken together, our results emphasize the importance of constant monitoring and the maintenance of COVID-19 prevention and control strategies.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e292021"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variants in the neutralization of fish oil to obtain effluent with fertilizing properties. 变体中和鱼油以获得具有施肥特性的流出物。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.290848

J A Legua Cárdenas, F E Airahuacho Bautista, J V Nunja Garcia, H G Villarreal Rodriguez, G M Gavedia Garcia, R J Hijar Guzman, J G Legua Angulo, Y J Vélez Chang

{"title":"Variants in the neutralization of fish oil to obtain effluent with fertilizing properties.","authors":"J A Legua Cárdenas, F E Airahuacho Bautista, J V Nunja Garcia, H G Villarreal Rodriguez, G M Gavedia Garcia, R J Hijar Guzman, J G Legua Angulo, Y J Vélez Chang","doi":"10.1590/1519-6984.290848","DOIUrl":"https://doi.org/10.1590/1519-6984.290848","url":null,"abstract":"Currently, an important trend in the agricultural activity is the development of organic agriculture. In this context, the application of a fertilizer derived from an effluent of the oilseed industry, to beet (Beta vulgaris L.), is evaluated, with the objective of improving the agronomic characteristics of beet and complementing the chemical deficiency of the soil in OM and N. The modified effluent is obtained from fish oil wash water, which contains nutrients, highlighting K, P and N. The experiment was conducted in a crop field, using completely randomized block design, with five treatments: T1, T2, T3, T4 and T5 with fertilizer doses of 0, 2, 3, 3, 4, 5 L/ha, respectively, applied to the beterraga. The results show a modified effluent with fertilizer properties, particularly for its high concentration of N (25.76 g/L) and its content in K and P. By changing the neutralizing agent and other complementary chemicals intended to confer fertilizer properties to the effluent produced in the oil refining process, a fertilizer was obtained that was applied to the beet crop in five treatments, highlighting the fourth treatment T4 with a yield of 23.97 t/ha.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e290848"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Βeta diversity of woody communities along Amazon forest succession: spatial and temporal decomposition. Βeta亚马逊森林演替过程中木本群落的多样性：时空分解。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.294251

R A Ibiapina, A A Conceição, J A A Meira-Neto, J S Costa, A J P Coelho, A C Rodrigues, W S Fonseca, R F Morais, F A Carvalho, L E Fernandes, L M Louzada-Camilo, G A Lima, S V Martins, A V Neri, L S Funch, P M Villa

引用次数: 0

Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses. 受氮钾比和生物刺激素剂量影响的甜椒叶绿素荧光

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.295013

A J Silva, T J Dias, J H B Silva, A C Bezerra, V F O Sousa, A H P C Martins, F S Bezerra, T I Silva, A V Silva, R N M Costa

{"title":"Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses.","authors":"A J Silva, T J Dias, J H B Silva, A C Bezerra, V F O Sousa, A H P C Martins, F S Bezerra, T I Silva, A V Silva, R N M Costa","doi":"10.1590/1519-6984.295013","DOIUrl":"https://doi.org/10.1590/1519-6984.295013","url":null,"abstract":"Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295013"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low sperm quality and increased testicular caspases in chronic stress mice induced by dexamethasone. 地塞米松致慢性应激小鼠精子质量下降和睾丸半胱天冬酶升高。

Brazilian Journal of Biology Pub Date : 2025-06-02 eCollection Date: 2025-01-01 DOI: 10.1590/1519-6984.289850

C Chaimontri, S Arun, T Kamollerd, N Lapyuneyong, C Taoto, S Innoi, T Chawalchitiporn, T Sawatpanich, N Uabundit, S Iamsaard

{"title":"Low sperm quality and increased testicular caspases in chronic stress mice induced by dexamethasone.","authors":"C Chaimontri, S Arun, T Kamollerd, N Lapyuneyong, C Taoto, S Innoi, T Chawalchitiporn, T Sawatpanich, N Uabundit, S Iamsaard","doi":"10.1590/1519-6984.289850","DOIUrl":"https://doi.org/10.1590/1519-6984.289850","url":null,"abstract":"Chronic stress (CS) from physical stressors and glucocorticoid administrations has been shown to induce germ cell apoptosis and low sperm quality. Among CS animal models, the effects of dexamethasone induced CS (DexCS) on testicular apoptosis and epididymal sperm parameters have not been fully demonstrated. This study aimed to investigate the changes of male reproductive system after CS induction by Dex. Adult male mice were divided into control and DexCS groups. Control mice were injected with sodium phosphate while DexCS mice were injected with DEX (4 mg/kg BW) for 21 consecutive days. The stress tests (sucrose preference, tail suspension, and forced swimming) were used to confirm CS behaviors. Sperm concentration, motility, morphology, viability, and acrosome status were assessed using computer-assisted semen analysis (CASA) and special staining. Histopathology of testis, epididymis, and penis was observed. Apoptotic protein expressions (Hsp70, caspases 3 and 9) in the testicular lysate were also determined. The results revealed that DexCS mice had significant increase of the immobility periods with decrease of total sucrose intake. DexCS significantly decreased sperm quality parameters particularly progressive motility. Testicular damages and decreased sperm mass in epididymis were obviously found in DexCS group. The expressions of testicular caspases 3 and 9, but not Hsp 70 were significantly increased in DexCS group compared to the control. It was concluded that DEX is a potential drug to induce chronic stress in mouse model, affecting male reproductive system via testicular histopathology and apoptotic pathway. Such effect may cause sperm physiology impairments like low progressive motile patterns.","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e289850"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0