Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Plasma steroid hormones concentrations and their relevance in the reproductive cycle of female Mountain Horned Lizard, Phrynosoma orbiculare","authors":"Laura Elena Hernández-Hernández ,&nbsp;Daniel Sánchez-Manjarrez ,&nbsp;Oswaldo Hernández-Gallegos ,&nbsp;Arturo Salame-Méndez ,&nbsp;Maricela Villagrán-Santa Cruz ,&nbsp;Kevin M. Gribbins ,&nbsp;Gisela Granados-González","doi":"10.1016/j.cbpa.2025.111833","DOIUrl":"10.1016/j.cbpa.2025.111833","url":null,"abstract":"<div><div>Reproduction is a fundamental trait in the life history of a species; therefore, it is of great interest at all levels within a species, including the individual, ecological and conservational scope. Hormonal studies in reproduction have become relevant because of their implications on the regulation of reproductive physiology. Herein we examined hormonal profiles of progesterone (P4), estradiol (E2) and testosterone (T) of adult female Mountain Horned Lizards, <em>Phrynosoma orbiculare</em>, from the Parque de la Ciencia Sierra Morelos, State of Mexico, Mexico. Hormonal concentrations were measured in the plasma of adult females monthly throughout a year and linked to the reproductive cycle of the species and environmental factors [mean monthly temperature (T°), total monthly rainfall, and daylight hours representing photoperiod]. We used direct ELISA technique to assess hormonal concentrations. We report that P4 is linked to pregnancy period, as maximum values of P4 are observed during the early gestational period and decrease to minimum levels during the late pregnancy phase and near the time of birth. An increase in E2 concentration occurs after pregnancy and parallels vitellogenesis in this species. Testosterone concentrations are at maximum levels during breeding and abruptly decrease to a minimum during late pregnancy. The correlation between P4 and T is positive while the relationship between P4 and E2 is negative, but no significant correlation between E2 and T is observed. Temperature and photoperiod have a negative correlation with P4 and a positive correlation with E2, while rainfall is not supported as an important environmental factor in the regulation of these hormones. Also, T does not seem to be affected by environmental factors revised in this study. A seasonal annual steroidogenic pattern is observable in female <em>P. orbiculare</em> that parallels its reproductive cycle characteristics and suggests an endogenous control of reproduction. However, it appears that certain environmental conditions, such as temperature and photoperiod, may influence the reproductive rhythmicity observed in female <em>P. orbiculare.</em></div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"304 ","pages":"Article 111833"},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological response and lipid metabolism induced by re-feeding in Jian carp (Cyprinus carpio var. Jian) subjected to short-term starvation","authors":"Yu Zhang ,&nbsp;Gang Jiang ,&nbsp;Wenrong Feng ,&nbsp;Yuanfeng Xu ,&nbsp;Jianlin Li ,&nbsp;Zhihua Zhang ,&nbsp;Yongkai Tang","doi":"10.1016/j.cbpa.2025.111832","DOIUrl":"10.1016/j.cbpa.2025.111832","url":null,"abstract":"<div><div>The effects of starvation and re-feeding on Jian carp (<em>Cyprinus carpio</em> var. <em>Jian</em>) remain incompletely understood. This study investigated changes in growth performance, liver antioxidant capacity, intestinal morphology, fatty acid profile, and expression of genes related to lipid metabolism. Juvenile <em>C. carpio</em> var. <em>Jian</em>, with initial body weight of 29.50 ± 2.00 g, were reared in 15 cylindrical culture barrels (200<em>L</em>) at a stocking density of 10 fish per barrel. These fish were subjected to five feeding regimes: a continuous feeding group (control group, treatment A), complete fasting (treatment B), 1, 2, and 3 days starvation within one week, followed by re-feeding 6 days (treatment C), 5 days (treatment D), and 4 days (treatment E). The results indicated significantly lower growth performance in <em>C. carpio</em> var. <em>Jian</em> in treatments B and E compared to the control and treatment C (<em>P</em> &lt; 0.05). Intestinal length (only 627 ± 13.14 μm in treatment B) was significantly reduced, and an increase in vacuoles was observed in <em>C. carpio</em> var. <em>Jian</em> with the prolonged starvation. Antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were significantly (<em>P</em> &lt; 0.05) improved in treatment C when compared with treatments B, D and E.. In contrast, malondialdehyde (MDA) value was lowest (12.62 ± 0.60 nmol/mL) in treatment B. Furthermore, continuous starvation significantly decreased the total lipid and fatty acids contents in the muscle (<em>P</em> &lt; 0.05). Compared with the control group, the reduction of total lipid and fatty acids contents was 31.53 % and 4.57 %, respectively, particularly affecting n3PUFA and n6PUFA. However, after one-day re-feeding, these contents resumed. Lipid metabolism is closely related to fish health, while in the current study, the genes related to lipid metabolism including lipoprotein lipase (<em>LPL</em>), 6-phosphogluconate dehydrogenase (<em>G6PD</em>), and peroxisome proliferator-activated receptor alpha (<em>PPARα</em>) did not differ significantly in treatment C compared to the control group (<em>P</em> &gt; 0.05). In contrast, expressions in treatments B, D, and E were significantly reduced (<em>P</em> &lt; 0.05). Taken together, prolonged starvation (&gt;one day per week) not only affected the growth, which may further disrupt the intestinal structure, weaken antioxidant capacity, but also attenuate lipid deposition.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"304 ","pages":"Article 111832"},"PeriodicalIF":2.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of ocean warming and elevated CO2 on the feeding behavior and physiology of two sympatric mesograzers","authors":"Ana Paula Ferreira ,&nbsp;Glauco Barreto de Oliveira Machado ,&nbsp;Juan Carlos Farias Pardo ,&nbsp;Alessandra Augusto ,&nbsp;Tânia Marcia Costa ,&nbsp;Fosca Pedini Pereira Leite","doi":"10.1016/j.cbpa.2025.111831","DOIUrl":"10.1016/j.cbpa.2025.111831","url":null,"abstract":"<div><div>Atmospheric CO₂ concentrations have increased significantly since pre-industrial times, leading to ocean warming and acidification. These environmental changes affect the physiology of marine organisms as they modify metabolic processes. Despite the critical role of temperature and pH in marine biology, studies of their combined effects are limited. This study investigated the interactive effects of ocean warming and acidification on the feeding behavior and physiology of two sympatric amphipods, <em>Hyale niger</em> and <em>Cymadusa filosa</em>. Using an orthogonal experimental design with two temperatures (27 °C and 30 °C) and two pH levels (7.8 and 7.5), we assessed feeding rates, respiration rates, ammonia excretion, and O/N ratios. Results indicated that <em>C. filosa</em> was less tolerant to these stressors than <em>H. niger</em>. While <em>H. niger</em> showed no significant changes between treatments, <em>C. filosa</em> showed reduced feeding rates and altered physiological responses to elevated temperature and decreased pH. Reducing the feeding rate of <em>C. filosa</em> may favor macroalgal biomass and strengthen bottom-up control in phytal communities. In addition, increased ammonia excretion in <em>C. filosa</em> suggests increased protein catabolism to meet energy demands at higher temperatures, despite reduced oxygen consumption. This indicates a compromised metabolism and a reduction in circulating oxygen capacity for <em>C. filosa</em>. The study shows heterogeneous responses to climate change, highlighting the need to assess combined environmental stressors in different species to accurately understand the impacts of climate change.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"304 ","pages":"Article 111831"},"PeriodicalIF":2.1,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing glucose acquisition strategies between two ancient fish species: Lake sturgeon (Acipenser fulvescens) and North Pacific spiny dogfish (Squalus suckleyi)","authors":"Jenna M. Drummond ,&nbsp;W. Gary Anderson ,&nbsp;Alyssa M. Weinrauch","doi":"10.1016/j.cbpa.2025.111829","DOIUrl":"10.1016/j.cbpa.2025.111829","url":null,"abstract":"<div><div>We assessed the functional role of the spiral valve in carbohydrate digestion and glucose acquisition during different feeding states in <em>Acipenser fulvescens</em> and <em>Squalus suckleyi</em>. For <em>S. suckleyi</em>, maltase activity was highest in the anterior and mid spiral valve, while sodium-glucose linked transporter 1 (<em>sglt1</em>) transcripts peaked in the mid spiral valve. Alongside these metrics, glucose tissue uptake demonstrated decreased maximal transport rates from 24 h to 7+ days post feeding, demonstrating a putative means for energy conservation. <em>A. fulvescens</em> showed the highest maltase activity and <em>sglt1</em> abundance in regions anterior to the spiral valve (pyloric ceca and anterior intestine). Additionally, glucose transport did not reach saturation in the spiral valve and anterior intestine of <em>A. fulvescens</em> over the measured concentrations, suggestive of a heightened capacity in these regions for glucose uptake. Overall, the spiral valve played a primary role in glucose digestion and transport in <em>S. suckleyi</em>, whereas <em>A. fulvescens</em> utilized the anterior intestine in addition to the spiral valve. Combined, these results also suggest regional functionality of carbohydrate acquisition within the spiral intestine in <em>S. suckleyi</em> but not in <em>A. fulvescens.</em> This demonstrates that the spiral valve may not always be the primary region of nutrient absorption in all species as suggested in the literature, highlighting the importance of comparing morphological and functional studies.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111829"},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From sensory organs to internal pathways: A comprehensive review of amino acid sensing in Drosophila","authors":"Quan Yi,&nbsp;Liangyao Xiong","doi":"10.1016/j.cbpa.2025.111828","DOIUrl":"10.1016/j.cbpa.2025.111828","url":null,"abstract":"<div><div>Organisms require various nutrients to provide energy, support growth, and maintain metabolic balance. Amino acid is among the most basic nutrients, serving as fundamental building blocks for protein synthesis while playing vital roles in growth, development, and reproduction. Understanding the mechanisms by which organisms perceive amino acids is key to unraveling how they select appropriate food sources and adapt to environmental challenges. The fruit fly, <em>Drosophila melanogaster</em>, serves as a powerful model for understanding fundamental genetic and physiological processes. This review focuses on recent advances in amino acid sensing mechanisms in <em>Drosophila melanogaster</em> and their relevance to feeding behavior, nutrient homeostasis, and adaptive responses, and integrates insights into peripheral sensory systems, such as the legs and proboscis, as well as internal regulatory mechanisms within the gut, fat body, and brain. It highlights key molecular players, including ionotropic receptors, gut-derived hormones, neuropeptides, and the microbiome-gut-brain axis. Additionally, the manuscript identifies knowledge gaps and proposes directions for future research, providing a comprehensive overview of this dynamic field.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111828"},"PeriodicalIF":2.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and physiological characterizations of razor clam (Sinonovacula constricta) aquaporin genes AQP4 and AQP10 in response to low-salinity tolerance","authors":"Geqi Gao ,&nbsp;Dong Yang ,&nbsp;Linyun Hu ,&nbsp;Liang Jia ,&nbsp;Donghong Niu","doi":"10.1016/j.cbpa.2025.111827","DOIUrl":"10.1016/j.cbpa.2025.111827","url":null,"abstract":"<div><div>Aquaporins (AQPs) are a family of membrane proteins responsible for the selective transport of water molecules and other neutral metabolic substances across cell membranes. These proteins play a crucial role in osmoregulation, enabling marine bivalves to accommodate salinity fluctuations. However, the regulatory mechanism of <em>AQPs</em> in the razor clam (<em>Sinonovacula constricta</em>) under salinity stress remain unclear. In this study, we investigated the roles of two classical <em>AQP</em> genes, Classical aquaporins <em>ScAQP4</em> and aquaglyceroporin <em>ScAQP10</em>, in response to hypotonic stress in <em>S. constricta</em>. <em><em>ScAQP4</em></em> and <em><em>ScAQP10</em></em> are hydrophobic proteins with six transmembrane domains and a highly conserved MIP structural motif. Upon acute hyposaline challenges, the expression of ScAQP4 and ScAQP10 in gills exhibited a significant increase in responses to low-salinity stress initially, followed by a gradual osmotic rebalance. To further investigate their biological functions, we conducted dsRNA interference to knockdown the expression levels of <em>ScAQP4</em> and <em>ScAQP10</em> in gill tissues and assessed the following physiological alternations. The knockdown of <em>ScAQP4</em> and <em>ScAQP10</em> resulted in a significant increase in heart rate and apoptosis and severe cellular damage of gills. These findings highlighted the critical roles of <em>ScAQP4</em> and <em>ScAQP10</em> in maintaining the osmotic balance of <em>S. constricta</em>. Collectively, these results propose a mechanism by which <em>S. constricta</em> regulates the expression of <em>AQPs</em> to accommodate salinity variations in the natural habitat.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111827"},"PeriodicalIF":2.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropeptide Y at the crossroads of male reproductive functions in a seasonally breeding reptile, Hemidactylus flaviviridis","authors":"Vishesh Chauhan ,&nbsp;Umesh Rai ,&nbsp;Mamta Tripathy ,&nbsp;Sunil Kumar","doi":"10.1016/j.cbpa.2025.111826","DOIUrl":"10.1016/j.cbpa.2025.111826","url":null,"abstract":"<div><div>Neuropeptide Y (NPY) is known to be a key regulator of reproductive functions across vertebrates. Its role in the modulation of gonadotropin releasing hormone (GnRH) has been extensively explored in mammals and fishes. However, no such report exists in aves, amphibians, or reptiles. Hence, the present study aimed to develop an insight into reproductive phase-dependent expression and role of NPY in the diencephalon region of male wall lizards. Expression of ligand, <em>npy,</em> and its receptor, <em>npyr</em> varied across the annual reproductive cycle of <em>Hemidactylus flaviviridis</em> with the highest expression of both observed during recrudescence. Further, the diencephalon region of recrudescent wall lizards treated with NPY demonstrated an increased mRNA level of <em>gnrh</em> and its receptor, <em>gnrhr</em>. In addition, the current study also elucidates hormonal regulation of diencephalonic <em>npy</em> and <em>npyr</em> wherein neuropeptides like kisspeptin and substance P, the gonadotropin, FSH, as well as sex steroids, dihydrotestosterone (DHT) and 17β-estradiol (E₂) inhibited <em>npy</em> and <em>npyr</em> expression in the diencephalon region of wall lizards. With regard to adipokines, leptin stimulated while nesfatin-1 inhibited diencephalonic <em>npy</em> and <em>npyr</em> expression. In conclusion, the current study is the first to present a comprehensive picture of reproductive phase-specific expression pattern, role, and hormonal regulation of neuropeptide Y in the diencephalon region of the male wall lizard, <em>H. flaviviridis</em>.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111826"},"PeriodicalIF":2.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The brine shrimp Artemia franciscana as a model for astrobiological studies: Physiological adaptations to Mars-like atmospheric pressure conditions","authors":"Maria Teresa Muscari Tomajoli ,&nbsp;Paola Di Donato ,&nbsp;Vincenzo Della Corte ,&nbsp;Giovanni Covone ,&nbsp;Gianluca Fasciolo ,&nbsp;Eugenio Geremia ,&nbsp;Adriana Petito ,&nbsp;Luca Tonietti ,&nbsp;Laura Inno ,&nbsp;Alessandra Rotundi ,&nbsp;Paola Venditti ,&nbsp;Gaetana Napolitano","doi":"10.1016/j.cbpa.2025.111825","DOIUrl":"10.1016/j.cbpa.2025.111825","url":null,"abstract":"<div><div>Understanding the adaptation mechanisms of extremophiles to extreme environments is fundamental to predicting organisms' capacity to survive in space and plan future space exploration missions. This study explores the physiological and metabolic adaptations of nauplii of a eukaryotic organism, <em>Artemia franciscana</em>, hatched from cysts exposed to Mars-like pressure conditions (6 mbar) by analyzing aerobic and anaerobic metabolism, mitochondrial function, and oxidative stress in nauplii. Mars-like pressure did not inhibit nauplii's hatching or <em>in vivo</em> respiration, indicating that the fundamental metabolic functions are preserved but affected cellular metabolism. The lower lactate levels suggested reduced anaerobic metabolism, and the reduction in the activity of Complex I of the electron transport chain, resulting in reduced <em>in vitro</em> respiration supported by pyruvate plus malate, suggested an effect on aerobic metabolism. However, the succinate-supported respiration remained stable according to unchanged Complex II activity. Changes in aerobic metabolism could affect Reactive Oxygen Species (ROS) production and management. We did not observe changes in ROS levels according to the unchanged activity of NADPH oxidase, a source of ROS in the early development stages of nauplii. A total antioxidant capacity reduction and increased susceptibility to oxidants were observed despite this. However, lipid and protein oxidative stress markers levels remained unchanged, likely due to the increased activity of antioxidant enzymes. Our results underscore the resilience of the cysts to Mars-like pressure conditions, indicating the potential of <em>Artemia franciscana</em> as a model organism in astrobiological research, opening new avenues for exploration in astrobiology.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111825"},"PeriodicalIF":2.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin modulates autophagy, mitochondria and antioxidant in the liver and brain of Perccottus glenni during recovery from freezing","authors":"Cunrun Ye,&nbsp;Zhaoyang Ning,&nbsp;Tingting Hu,&nbsp;Xiaoyu Zhao,&nbsp;Weijie Mu","doi":"10.1016/j.cbpa.2025.111824","DOIUrl":"10.1016/j.cbpa.2025.111824","url":null,"abstract":"<div><div>The Amur sleeper (<em>Percottus glenii</em>), a freeze tolerant fish species, can survive being frozen in ice, survival after recovery from freezing. This study investigated the role of melatonin in the recovery of <em>Perccottus glenni</em> following freezing. The fish were categorized into the following groups: non-treated control (Con), pinealectomy (Px), melatonin injection (Mlt), sham control (Sham), and saline injection control (Sal). The results revealed that the melatonin levels were affected by pinealectomy and melatonin injection. The liver and brain of the Px and Mlt groups exhibited autophagy after 4 h, along with the differential expression of endoplasmic reticulum stress-related genes. Furthermore, the activities of the mitochondrial complex initially increased at 4 h and then decreased by 12 h in the Px and Mlt groups, while antioxidant enzyme activities varied across groups and time points. These findings indicated that melatonin plays a key role in the recovery of <em>P. glenni</em> in a time-dependent manner, affecting autophagy, mitochondrial function, and antioxidant capacity. This study is the first to demonstrate melatonin's time-dependent role in facilitating the recovery of <em>P. glenni</em> after freezing, highlighting its critical involvement in modulating autophagy, mitochondrial function, and antioxidant processes. These findings shed light on the physiological mechanisms underlying freeze tolerance and recovery in fish, offering valuable insights for understanding and potentially enhancing the recovery processes in other species.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111824"},"PeriodicalIF":2.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting the effects of immobilisation and anaesthesia on the stress physiology and behaviour of juvenile lake sturgeon (Acipenser fulvescens)","authors":"Connor H. Reid ,&nbsp;Raegan Davis ,&nbsp;Kathleen M. Gilmour ,&nbsp;Cheryl N. Klassen ,&nbsp;James A. Crossman ,&nbsp;Steven J. Cooke","doi":"10.1016/j.cbpa.2025.111823","DOIUrl":"10.1016/j.cbpa.2025.111823","url":null,"abstract":"<div><div>Anaesthesia and/or immobilisation are typically required to facilitate experimental procedures on fishes such as the surgical implantation of electronic tags. Yet, optimal anaesthetic or immobilisation methods have primarily been developed and tested in teleosts, with relevant information on efficacy and suitability of these methods lacking for basal ray-finned fishes such as imperilled sturgeons (Acipenseridae). Here, we investigated the behavioural and physiological responses of hatchery-origin juvenile lake sturgeon (<em>Acipenser fulvescens</em>) to four immobilisation or anaesthetic methods. We conducted an experiment wherein fish were held in a fashion designed to induce tonic immobility, exposed to electroanaesthesia with a TENS unit, anaesthetised with MS-222, or anaesthetised with clove oil, and then subjected to a simulated intracoelomic tagging surgery. Following reflex indicator scoring, fish were “released” into a circular tank arena for behaviour trials and blood sampling at 0.5, 2, and 4 h post-release. Both tonic immobility and electroanaesthesia were ineffective at immobilising fish, and surgery under these methods led to relatively little behavioural impairment but the highest plasma cortisol peaks. Fish anaesthetised with MS-222 or clove oil had greater post-release behavioural impairment but lower cortisol levels. We discuss the implications of our findings for the practical application of these methods, and the challenges with identifying the most suitable method for immobilising or anaesthetising sturgeons in laboratory and field settings.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"303 ","pages":"Article 111823"},"PeriodicalIF":2.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}