Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Characterization of Transient Receptor Potential Cation Channel subfamily Vanilloid 4 (TRPV4) in chub mackerel: Role in low-salinity acclimation and reproductive implications","authors":"Kyle Dominic Barnuevo ,&nbsp;Nguyen The Vuong ,&nbsp;Sipra Mohapatra ,&nbsp;Oga Sato ,&nbsp;Hironori Katoh ,&nbsp;Takehiko Itoh ,&nbsp;Naoki Nagano ,&nbsp;Michiya Matsuyama ,&nbsp;Tapas Chakraborty ,&nbsp;Kohei Ohta","doi":"10.1016/j.cbpa.2025.111879","DOIUrl":"10.1016/j.cbpa.2025.111879","url":null,"abstract":"<div><div>Salinity plays a crucial role in fish physiology, including osmoregulation, metabolism, growth, reproduction, and disease resistance. In this study, we characterized the Transient Receptor Potential Cation Channel Subfamily Vanilloid 4 (<em>trpv4</em>) gene and investigated the effects of low-salinity conditions on its expression in various tissues of chub mackerel (<em>Scomber japonicus</em>), an economically important aquaculture species. The <em>trpv4</em> open reading frame (ORF) consists of 2616 nucleotides and encodes an 872-amino acid protein. It shares up to 94.01 % phylogenetic similarity with <em>Thunnus maccoyii</em> and possesses 6 transmembrane domains highly conserved with human TRPV4. <em>Trpv4</em> was widely distributed across most organs and showed significant sex-biased expression. <em>In situ</em> hybridization data showed that <em>trpv4</em> was explicitly expressed in various parts of the pituitary, from <em>pars intermedia</em> (PI) to <em>rostral pars distalis</em> (RPD) where it colocalized with <em>prolactin 1</em> (<em>prl1</em>) in RPD. Furthermore, exposure to decreasing salinity from 35 ppt to 10 ppt demonstrated a significant upregulation of <em>trpv4</em> transcription in the pituitary, gill, skin, and gonad under low-salinity conditions. Although <em>prl1</em> was upregulated, <em>follicle-stimulating hormone beta subunit</em> (<em>fshb</em>) expression was decreased in the pituitaries of low-salinity groups. These findings suggest that <em>trpv4</em> is regulated by salinity in chub mackerel and may play a role in reproductive responses to salinity changes. Overall, these results highlight the characterization of <em>trpv4</em> in marine fish and its potential roles in osmoregulation, as well as in transmitting low-salinity signals to reproduction-related organs. The involvement of the brain–pituitary–gonad axis in this response warrants further investigation.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111879"},"PeriodicalIF":2.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal regulation and PIP2 dependence of inwardly rectifying potassium channels (Kir) in retinal horizontal cells of goldfish (Carassius auratus)","authors":"Shilo L. Rousseau ,&nbsp;Michael G. Jonz","doi":"10.1016/j.cbpa.2025.111877","DOIUrl":"10.1016/j.cbpa.2025.111877","url":null,"abstract":"<div><div>Many species face extreme changes in their environment throughout the year, during which time they must modify their neuronal activity to survive. Horizontal cells (HCs) are interneurons in the goldfish (<em>Carassius auratus</em>) retina that demonstrate seasonal changes in membrane structure and excitability. Excitability in neurons is strongly influenced by inwardly rectifying potassium channels (K_ir), suggesting that K_ir in HCs may be a candidate for modulating seasonal changes. K_ir channel function depends on the plasma membrane phospholipid, PIP₂, to which it is bound. However, it remains unclear whether the PIP₂-K_ir channel interaction contributes to regulation of K_ir in goldfish HCs. Using perforated patch voltage-clamp recording, we identified K_ir current by inhibition with cesium (Cs⁺) and compared peak inward K_ir current density in goldfish HCs isolated during the summer and winter months. Significantly more current was inhibited by Cs⁺ in winter HCs, suggesting more open K_ir channels. To examine the role of PIP₂ in regulating K_ir, tamoxifen or activation of phospholipase C by m-3M3FBS were used to disrupt the PIP₂-K_ir channel interaction and subsequently reduced K_ir current. Spermine, through strengthening the PIP₂-K_ir interaction, reduced the inhibitory effects of tamoxifen. Additionally, in current-clamp recordings, HCs treated with m-3M3FBS displayed a depolarized membrane potential (V_m) and a decrease in spontaneous Ca²⁺-based action potentials. We demonstrate that K_ir activity is seasonally regulated in goldfish HCs and that K_ir is dependent upon PIP₂. Our results suggest PIP₂ as a potential target for coordinating seasonal changes in the activity of neurons in the goldfish retina.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111877"},"PeriodicalIF":2.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anorexigenic and anxiogenic effects of the plasticiser DEHP (di-2-ethylhexyl phthalate) in goldfish: Involvement of PPAR signalling and feeding-related neuropeptides","authors":"Lisbeth Herrera-Castillo,&nbsp;Claudia Hernández-Villasevil,&nbsp;André Barany,&nbsp;Miguel Gómez-Boronat,&nbsp;Esther Isorna,&nbsp;Nuria de Pedro","doi":"10.1016/j.cbpa.2025.111878","DOIUrl":"10.1016/j.cbpa.2025.111878","url":null,"abstract":"<div><div>Di-2-ethylhexyl phthalate (DEHP), a widely used plasticiser, is a pervasive environmental contaminant with potential detrimental effects on aquatic organisms. The objective of this study was to provide an integrative analysis of how DEHP alters energy balance, temporal homeostasis and fish welfare — interrelated aspects critical to animal survival — to address critical gaps in our understanding of its toxicological effects. Goldfish (<em>Carassius auratus</em>) were chronically (14 days) treated with DEHP. Energy balance was assessed through locomotor activity, metabolic rate, feed intake, and growth indices. Daily of locomotor and metabolic rate rhythms were examined to explore potential circadian disruptions. Anxiety-like behaviours were also examined to assess welfare. DEHP decreased feed intake and food-anticipatory activity (FAA), suggesting an anorexigenic effect, which may have been mediated by increased expression of anorexigenic genes in the hypothalamus and liver, along with decreased expression of orexigenic <em>npy</em> (neuropeptide Y) gene in the hypothalamus. Growth parameters remained unchanged, probably due to compensatory reductions in energy expenditure, as indicated by decreased locomotor activity and metabolic rate. Daily rhythms in these two parameters were preserved, suggesting no disruption in temporal homeostasis. DEHP increased hepatic expression of peroxisome proliferator-activated receptor (PPAR)-related genes, suggesting that PPARs activation is a potential mode of action for DEHP in fish. Anxiety levels were elevated, as evidenced by increased thigmotaxis and scototaxis in behavioural tests, which may be mediated by changes in hypothalamic neuropeptides. These findings highlight the adverse effects of DEHP on energy regulation and animal welfare, providing novel insights into its broader physiological consequences in fish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111878"},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complementary DNA (cDNA) cloning and expression analysis of selenium glutathione peroxidase (Se-GPx) and glutathione S-transferases (GST) 1 in red claw crayfish (Cherax quadricarinatus)","authors":"Jia-Han Liu ,&nbsp;Jun-Tao Li ,&nbsp;Yao-Peng Lu ,&nbsp;Pei-Hua Zheng ,&nbsp;Ze-Long Zhang ,&nbsp;Xiu-Xia Zhang ,&nbsp;Chi Xu ,&nbsp;Zi-Hang Yu ,&nbsp;Xiu-Ying Yang ,&nbsp;Hui Guo ,&nbsp;Jian-An Xian","doi":"10.1016/j.cbpa.2025.111876","DOIUrl":"10.1016/j.cbpa.2025.111876","url":null,"abstract":"<div><div>Selenium glutathione peroxidase (Se-GPx) and Glutathione S-transferases (GST) are crucial parts of cellular detoxification systems that protect cells from reactive oxygen species (ROS). In this study, cDNA sequences of CqGST1 and CqSe-GPx were cloned from red claw crayfish (<em>Cherax quadricarinatus</em>). The molecular masses of the predicted protein are 25.36 and 21.49 kDa, respectively. CqGST1 and CqSe-GPx have open reading frames (ORFs) of 657 bp and 570 bp and encode peptides of 218 and 189 amino acids, respectively. Sequence alignment showed that the amino acid sequence of CqSe-GPx identity with that of the <em>Exopalaemon carinicauda</em>, <em>Penaeus japonicus</em>, <em>Macrobrachium rosenbergii</em>, <em>Penaeus monodon</em>, <em>Litopenaeus vannamei</em>, <em>Macrobrachium nipponense</em> and <em>Scylla paramamosai</em>. The amino acid sequence of CqGST1 identity with <em>Procambarus clarkii</em>, <em>Stegastes partitus</em>, <em>Gavialis gangeticus</em>, <em>Alligator mississippiensis,</em> and L. <em>vannamei</em>. The qRT-PCR results showed that CqSe-GPx and CqGST1 were found in a variety of tissues, with the highest hemolymph expression and the weakest expression in muscle, eye stalk, and heart. The expression levels of CqSe-GPx and CqGST1 in the hepatopancreas and gills of crayfish were detected under ammonia-N and microcystins-LR stresses. The expression of CqSe-GPx was induced in the hepatopancreas of crayfish during MC-LR stress, and gill CqSe-GPx expression levels were induced at 6 and 12 h. Hepatopancreas and gill CqSe-GPx expression levels were significantly up-regulated during ammonia-N at 24 and 48 h, respectively. The CqGST1 expression levels were significantly elevated in the hepatopancreas and gill during MC-LR or ammonia-N stress. Under ammonia stress, hepatopancreas and gill GST expression levels of crayfish showed a decreasing and then increasing trend. Hepatopancreas and gill CqGST1 expression levels of crayfish were significantly up-regulated at 12, 24, and 48 h of ammonia-N stress. These results suggest that CqSe-GPx and CqGST1 play an important role in protecting <em>C. quadricarinatus</em> from ammonia-N and MC-LR stress and provide a theoretical basis for further study on the molecular mechanism of response to environmental stress on crayfish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111876"},"PeriodicalIF":2.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acidification affects the early development of Colombian endemic fish Prochilodus magdalenae","authors":"Villamizar Natalia ,&nbsp;Guardiola Jose Luis ,&nbsp;Cruz-Botto Sara ,&nbsp;Rodríguez Hector Alejandro ,&nbsp;Reyes-Bermúdez Alejandro ,&nbsp;Noisette Fanny","doi":"10.1016/j.cbpa.2025.111875","DOIUrl":"10.1016/j.cbpa.2025.111875","url":null,"abstract":"<div><div>There is a significant knowledge gap regarding the acidification of freshwater ecosystems and its effects on biological systems. The bocachico (<em>Prochilodus magdalenae</em>), an endemic and migratory species vital to Colombia's inland fisheries, is currently classified as vulnerable. This study evaluated the impact of different pH levels (6.2, 7.2, and 7.6), previously recorded in the species' natural habitat, on its early development. Using an automated IKS Aquastar system, embryo incubation and larval maintenance were monitored from 0 to 5 days post-fertilization, assessing development, hatching, and survival at both organismal and transcriptional levels. Embryos exposed to pH 6.2 showed delayed development within 4 h post-fertilization, the lowest hatching rate (68.33 ± 3.13 %), and survival (23.88 ± 4.53 %), along with the highest incidence of malformations (37.80 ± 4.4 %). The pH 7.6 group also showed adverse effects, but to a lesser extent. Transcriptome analysis revealed a distinct molecular response in the pH 6.2 group, identifying 1214 differentially expressed genes related to early development, ossification, organ formation, sensory systems, and cellular processes. The findings indicate that pH fluctuations previously observed in the species' natural environment significantly affect <em>P. magdalenae</em> during early life stages, which raises serious concerns about the long-term viability of this endemic species and the sustainability of the artisanal fisheries that depend on it.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111875"},"PeriodicalIF":2.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of Atlantic salmon to long-term sustained aerobic training at suboptimum elevated temperature: Cardiac anatomy, aerobic performance, and growth implications","authors":"Marco Foddai ,&nbsp;Chris G. Carter ,&nbsp;Andrew T. Wood ,&nbsp;Kelli C. Anderson ,&nbsp;Jayson M. Semmens","doi":"10.1016/j.cbpa.2025.111874","DOIUrl":"10.1016/j.cbpa.2025.111874","url":null,"abstract":"<div><div>Sustained aerobic training is suggested to enhance cardiac performance and growth in farmed salmonids, but its effects under suboptimum elevated temperatures remain unclear. This study examined whether continuous training at different temperatures could improve growth and whether it influenced cardiac performance at a suboptimum elevated temperature in a context relevant to offshore aquaculture. Atlantic salmon were reared for 90 days at 15 °C (control temperature) and 20 °C (suboptimum elevated temperature), with either continuous aerobic swimming (trained: 0.45 m.s⁻¹) or standard conditions (untrained: 0.18 m.s⁻¹). Growth and feed intake were assessed at both temperatures, while cardiac and metabolic parameters were measured only at 20 °C.</div><div>At 15 °C, trained fish exhibited increased feed intake, but this did not translate into improved growth. At 20 °C, neither feed intake nor growth improved with training. Swim-tunnel respirometry at 20 °C revealed no significant differences in aerobic performance between trained and untrained fish, although trained fish exhibited lower interindividual variability in metabolic and swimming parameters. While training increased relative ventricular mass (RVM), indicating potential cardiac remodelling, this did not result in improved metabolic performance.</div><div>These findings underscore the complexity of physiological responses to exercise and temperature in salmon aquaculture. While continuous aerobic training induced minor cardiac adaptations, its impact on growth and performance was limited, particularly at suboptimum elevated temperatures. This research provides valuable insights into how Atlantic salmon may respond to offshore farming environments, with specific relevance to Tasmania's aquaculture industry.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111874"},"PeriodicalIF":2.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous water loss and epidermal lipid content in lanceheads (Crotalinae: Bothrops)","authors":"Clara Carrero C. Casetta ,&nbsp;Yanca A. Salomoni ,&nbsp;Nathie M. Batista ,&nbsp;Sávio S. Sant' Anna ,&nbsp;Daniel R. Stuginski","doi":"10.1016/j.cbpa.2025.111872","DOIUrl":"10.1016/j.cbpa.2025.111872","url":null,"abstract":"<div><div>Cutaneous evaporation is the primary route of water loss in snakes. Species from arid environments typically show lower cutaneous water loss (CWL) rates due in part to higher epidermal lipid content. However, past comparative studies have used distantly related taxa or lacked phylogenetic comparative methods, limiting their conclusions. We measured CWL and lipid content (LC) in shed skins of eight lancehead species (<em>Bothrops</em>) from habitats with varying canopy coverage. Using phylogenetic comparative methods, we examined the effects of canopy coverage, snout-vent length (SVL), sex, and species on CWL and assessed its correlation with LC. Our results show that canopy coverage significantly influences CWL, independent of phylogeny. Snakes from open habitats exhibited lower CWL rates than those restricted to forests, which showed greater individual variation. Species from the most xeric habitat (Caatinga) displayed the lowest CWL. Neither sex nor SVL affected CWL, and phylogenetic signal was negligible (λ ≈ 0), suggesting weak phylogenetic constraints. CWL and LC were inversely correlated, with LC higher in open-habitat species. These findings indicate that habitat type, particularly canopy coverage, drives interspecific variation in CWL among closely related species, while phylogenetic effects are more relevant at higher taxonomic levels. Lipid content in the epidermis plays a critical role in regulating CWL, though differences in lipid composition may also contribute.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111872"},"PeriodicalIF":2.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasting elicits immune modulation and leukocyte redistribution in bullfrogs (Lithobates catesbeianus)","authors":"Aymam C. de Figueiredo ,&nbsp;Débora M.A. Prado ,&nbsp;Felipe R. Floreste ,&nbsp;Patrício G. Garcia Neto ,&nbsp;Fernando R. Gomes ,&nbsp;José E. de Carvalho","doi":"10.1016/j.cbpa.2025.111873","DOIUrl":"10.1016/j.cbpa.2025.111873","url":null,"abstract":"<div><div>An integrated range of behavioral, physiological, and biochemical adjustments reduces metabolic expenditure and energy utilization during fasting to maintain homeostasis. In order to cope with these adjustments, several vertebrates can modulate immune function and corticosterone secretion while fasting to save energy. However, in ectothermic animals that can fast for longer periods due to their low metabolic rate, the underlying corticosterone and immune modulation is still not well understood. This study aimed to investigate corticosterone and immune modulation during fasting in the bullfrog (<em>Lithobates catesbeianus</em>). Bullfrogs were divided into two treatments: a ‘control group’, fed twice a week with fish feed for 31 days; and a ‘fasting group’, which remained fasting for 31 days. On the 31st day of experiment, blood was collected to measure corticosterone plasma levels and immune function (neutrophil/lymphocyte ratio, plasma bacterial killing ability - BKA, and hemagglutination) variables. Fasting did not affect corticosterone plasma levels but increased neutrophil/lymphocyte ratio and hemagglutination, while BKA was decreased. In this way, fasting modulates the innate immune function in male bullfrogs by increasing the NL ratio and hemagglutination activity while reducing BKA, without affecting plasma CORT levels or fat body mass content. Thus, 31 days of fasting induces blood immune cell redistribution and an immunomodulatory response without significant activation of the HPI axis or depletion of energy stores.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111873"},"PeriodicalIF":2.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in thermal preference, respiratory and metabolic patterns in Tropidurus torquatus: Population responses from two distinct Brazilian biomes","authors":"Sofia L. B. da S. Matos ,&nbsp;Lays de Souza Porto ,&nbsp;Melissa Bars-Closel ,&nbsp;Fábio Cury de Barros ,&nbsp;Tiana Kohlsdorf ,&nbsp;Kenia C. Bícego ,&nbsp;Luciane H. Gargaglioni","doi":"10.1016/j.cbpa.2025.111871","DOIUrl":"10.1016/j.cbpa.2025.111871","url":null,"abstract":"<div><div>Variations in structural and climatic conditions of habitats, particularly in environmental temperatures, emerge as a pivotal determinant exerting broad influence on physiological processes, such as modifications in ventilation and metabolism within certain species. We investigated the thermal preference, ventilatory, and metabolic responses to acclimation in two Brazilian populations of the lizard <em>Tropidurus torquatus</em> — one population from the Pampa biome and the other from the restinga (sandbank) ecosystems in the Atlantic Forest biome. We divided lizards into three distinct acclimation temperatures (20 °C – ‘cold’, 25 °C – ‘warm’, and 30 °C – ‘hot’ regimes) and, after three weeks, recorded their preferred temperatures. Subsequently, we measured ventilation and metabolism in the same individuals at 20 °C, 25 °C, and 30 °C. Both populations exhibited similar responses to acclimation in thermal variables. However, in the extreme acclimation regimes, the Pampa population selects colder temperatures more frequently than the Restinga population. We did not detect any effect of acclimation or population on ventilation. In contrast, we identified changes in respiratory frequency likely related to changes in acclimation temperature regimes for both populations. In terms of metabolism, we recorded lower oxygen consumption in the Pampa population at warm and hot acclimation regimes. Our findings suggest that physiological responses to acclimation in <em>T. torquatus</em> may be intricately molded by the specific environmental contexts in which populations are situated. Animals originating from the southern population exhibit increased thermal responses – like the reduction of O₂ consumption when faced with 30 °C experimental temperature – potentially attributed to their exposure to more variable temperature regimes in their native habitat.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111871"},"PeriodicalIF":2.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyamine dynamics and transcriptomic changes in honey bees (Apis mellifera, L.) during aging","authors":"Jelena Purać ,&nbsp;Elvira Vukašinović ,&nbsp;Marko Kebert ,&nbsp;Tatjana Čelić ,&nbsp;Srđana Đorđievski ,&nbsp;Jelena Spremo ,&nbsp;Ivan Pihler ,&nbsp;Danijela Kojić","doi":"10.1016/j.cbpa.2025.111870","DOIUrl":"10.1016/j.cbpa.2025.111870","url":null,"abstract":"<div><div>Honey bees (<em>Apis mellifera,</em> L.) play an important role in ecosystems due to their pollination, which directly impacts biodiversity and agricultural production. Understanding the biological mechanisms that determine bee aging is important for conserving bee populations and the ecosystems they serve. This study explored the dynamics of polyamine level changes and transcriptomic alterations during the aging of summer and winter worker bees. The present study's results indicate that the polyamine content in summer bees decreased overall with age, while winter bees exhibited a biphasic pattern, with an initial decrease followed by an increase in older individuals. These differences likely reflect distinct physiological needs during summer (work-intensive) and winter (survival-focused) periods. Transcriptomic analyses identified age-related changes, with key driver gene analysis highlighting critical genes associated with cuticle formation, venom activity, and polyamine metabolism, indicating structural and metabolic adaptations with age. KEGG pathway analysis identified enrichments in pathways related to peroxisome function, lipid metabolism, glycan degradation, lysosomal activity, and others underscoring shifts in cellular maintenance and energy regulation. This study underscores the complexity of polyamine dynamics and molecular adaptations in honey bee aging and provides a foundation for conserving bee populations and better understanding longevity across species.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"306 ","pages":"Article 111870"},"PeriodicalIF":2.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}