Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"The effects of individual differences in anxiety on anti-predator responses, thermal and hypoxia tolerance in zebrafish (Danio rerio)","authors":"Xiao-hong Li,&nbsp;Cheng Fu,&nbsp;Xue-ting Tan,&nbsp;Shi-jian Fu","doi":"10.1016/j.cbpa.2025.111899","DOIUrl":"10.1016/j.cbpa.2025.111899","url":null,"abstract":"<div><div>Anxiety, as a crucial stress response to potential threats in animals, exhibits significant individual differences, with high-anxiety individuals typically allocating greater attentional resources to threat detection. We hypothesized that such individuals would demonstrate enhanced threat sensitivity and anti-predator responses, but compromised stress tolerance due to constrained energy allocation. Using the novel tank diving test, we screened zebrafish (<em>Danio rerio</em>) with high- and low-anxiety phenotypes and compared their anti-predator responses (anxiety-like behaviors, metabolic rates, and predator avoidance behaviors) under predator chemical or visual cues, along with their thermal and hypoxia tolerance capacities under baseline conditions. The results demonstrate that high-anxiety zebrafish exhibit enhanced anti-predator responses in threatening environments, while showing no compromised stress tolerance at baseline. These findings demonstrate the adaptive superiority of the high-anxiety phenotype in high-predation-risk environments, providing theoretical foundations for understanding the evolutionary role of anxiety in fish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111899"},"PeriodicalIF":2.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Temporal changes in PTTH/Egf signaling and ERK target gene expressions during chilling-induced diapause termination in Bombyx mori eggs” [Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Volume 307, September 2025, 111,884].","authors":"Shi-Hong Gu","doi":"10.1016/j.cbpa.2025.111895","DOIUrl":"10.1016/j.cbpa.2025.111895","url":null,"abstract":"","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111895"},"PeriodicalIF":2.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the fatty acid composition of Pacific bluefin tuna Thunnus orientalis larvae by body part and depending on growth rate","authors":"Yuko Hiraoka ,&nbsp;Hiroshi Hashimoto ,&nbsp;Tetsuya Takatsu ,&nbsp;Yuki Koide ,&nbsp;Yosuke Tanaka ,&nbsp;Yasuhiro Ando","doi":"10.1016/j.cbpa.2025.111897","DOIUrl":"10.1016/j.cbpa.2025.111897","url":null,"abstract":"<div><div>In this study, we aimed to elucidate the characteristics of faster-growing Pacific bluefin tuna (<em>Thunnus orientalis)</em> larvae by focusing on the localization of long-chain polyunsaturated fatty acids in different body parts and at various days post-hatch (dph). To accomplish this, we compared low- and high-growth-rate groups of larvae under mass-rearing conditions. Multivariate analysis revealed that the first axis separated the dph and growth groups, and the high-growth-rate group tended to be distributed on the same side at 20 dph. This suggests that the faster-growing larvae had switched their diet sooner after the change in feeding regime, either from rotifers to yolk-sac larvae of the spangled emperor (<em>Lethrinus nebulosus</em>) or from yolk-sac larvae to a commercial diet. However, the fatty acid compositions differed significantly between the two growth groups at 10 dph, before the change in feeding regime (PERMANOVA; <em>P</em> &lt; 0.001). Notably, %DHA was higher in the head (mean: 24.4 %) and eyes (29.2 %) of the high-growth group, while %DHA levels in the trunk remained similar between groups (range: 23.1 %–23.7 %). These findings suggest that early accumulation of DHA (approximately 30 %) in the eyes, in contrast to the lower requirement in trunk tissues, may be critical for sustaining rapid growth in Pacific bluefin larvae during the pre-diet-shift period.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111897"},"PeriodicalIF":2.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual differences in response to a chronic high-fat diet in striped hamsters (Cricetulus barabensis)","authors":"Wenting Li ,&nbsp;Binxin Yin ,&nbsp;Xinyuan Dong ,&nbsp;Boyang Ding ,&nbsp;Jing Wen","doi":"10.1016/j.cbpa.2025.111896","DOIUrl":"10.1016/j.cbpa.2025.111896","url":null,"abstract":"<div><div>Adult male striped hamsters (<em>Cricetulus barabensis</em>) were fed an HFD for 12 weeks to examine individual differences in response to chronic exposure to a high-fat diet (HFD). The hamsters were subsequently assigned to three experimental groups according to those that exhibited body weight gain (GW), a stable body weight (SW), or body weight loss (LW). The GW group showed a profile of HFD-induced obesity, with the highest levels of energy intake, fat content, brown adipose tissue (BAT) mass, <em>UCP1</em> gene expression in the BAT, and lipid metabolism. The SW group was resistant to HFD, showing lower levels of the above-mentioned markers than those of the GW group. In addition, the LW group appeared to be aversive to HFD, displaying a negative body mass gain compared to the other two groups. Group differences in the relative abundance of several strains of the gut microbiota were also observed, suggesting the potential involvement of the gut microbiota in individual variation in the sensitivity, resistance, and aversive responses to HFD-induced obesity. These data demonstrate the utility of the striped hamster model in functional studies of the mechanisms underlying HFD-induced obesity and its potential treatment and prevention.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111896"},"PeriodicalIF":2.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Melatonin modulates autophagy, mitochondria and antioxidant in the liver and brain of Perccottus glenni during recovery from freezing” [Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 303, 2025, 111824].","authors":"Cunrun Ye,&nbsp;Zhaoyang Ning,&nbsp;Tingting Hu,&nbsp;Xiaoyu Zhao,&nbsp;Weijie Mu","doi":"10.1016/j.cbpa.2025.111894","DOIUrl":"10.1016/j.cbpa.2025.111894","url":null,"abstract":"","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111894"},"PeriodicalIF":2.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of acute environmental challenges on the expression of peptide hormone-encoding genes in the zebrafish caudal neurosecretory system","authors":"Bérénice Bichon,&nbsp;Gladys Alfama,&nbsp;Anne-Laure Gaillard,&nbsp;Hervé Tostivint,&nbsp;Guillaume Pézeron","doi":"10.1016/j.cbpa.2025.111893","DOIUrl":"10.1016/j.cbpa.2025.111893","url":null,"abstract":"<div><div>The caudal neurosecretory system (CNSS) is a neuroendocrine complex unique to fish, first described in 1955. Since then, it has been hypothesized to contribute to several physiological processes, but its real functions remain largely unclear. However, so far, the many studies devoted to it agree that it could play an important role in osmoregulation. More recently, it has also been suggested that it could be involved in thermal adaptation. The zebrafish (<em>Danio rerio</em>) is a well-established model organism for functional studies. Yet so far, the functions of the CNSS have not been thoroughly investigated in this species. As a first step in such investigations, the present study aimed to identify environmental factors whose variations induce changes in CNSS endocrine activity. For this purpose, juvenile zebrafish were submitted to acute (2, 8, and 24 h) pH, salinity, and temperature challenges. As indicators of the CNSS endocrine activity, the expression levels of peptide hormone-encoding genes known or suspected to be synthesized in the CNSS were measured using quantitative PCR. The genes selected for this study were as follows: <em>corticotropin-releasing hormone b</em> (<em>crhb</em>), oxytocin (<em>oxt</em>), <em>proenkephalin</em> (<em>penka</em> and <em>penkb</em>), <em>parathyroid hormone-like hormone</em> (<em>pthlha</em> and <em>pthlhb</em>), <em>stanniocalcin 2</em> (<em>stc2a</em> and <em>stc2b</em>), <em>urotensin 1</em> (<em>uts1</em>) and <em>urotensin 2</em> (<em>uts2a</em> and <em>uts2b</em>). Our findings revealed that the pH challenge affected the expression of three genes – <em>crhb</em>, <em>penka,</em> and <em>stc2b -</em> and the salinity challenge altered four genes - <em>oxt, uts1, uts2a</em>, and <em>uts2b</em> - while the temperature challenge modified the expression of all genes of our panel. These results indicated that the zebrafish CNSS is sensitive to changes in these environmental parameters and support the use of the zebrafish as a relevant model for studying the functions of the CNSS.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111893"},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth rate is associated with reduced oxidative stress and this effect is modulated by the degree of social dominance in males of an African cichlid fish","authors":"Peter D. Dijkstra","doi":"10.1016/j.cbpa.2025.111892","DOIUrl":"10.1016/j.cbpa.2025.111892","url":null,"abstract":"<div><div>Attaining large body size has several selective benefits, however, increased growth rate has potential costs that can constrain investment in other life history traits, such as reproductive output and territorial defense. Oxidative stress can both constrain and result from growth, potentially mediating life history trade-offs between growth rate and other life history traits. Studies on the oxidative cost of growth have provided mixed evidence, in part because components of oxidative balance, including oxidative damage and antioxidant function, is influenced by investment in other activities in a tissue-specific manner. Here, I examined how among-individual variation in growth rate is linked to oxidative stress, and how this relationship is influenced by markers of social dominance (aggressiveness and relative gonad size) in males of the cichlid fish <em>Astatotilapa burtoni</em>. To this end, 7 markers of oxidative damage and antioxidant function in various tissue types (total of 14 measurements) were assessed in dominant and subordinate males. I found that dominant males grew faster than subordinate males. However, increased growth was linked to reduced oxidative stress. This effect was independent of social status but modulated by the degree of social dominance. Overall, the results are consistent with oxidative stress mediating the link between growth and other life history traits. However, my findings challenge the idea that increased growth rate results in elevated oxidative stress, perhaps due to effective protective mechanisms that can neutralize the oxidative challenge of growth.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111892"},"PeriodicalIF":2.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal changes in PTTH/Egf signaling and ERK target gene expressions during chilling-induced diapause termination in Bombyx mori eggs","authors":"Shi-Hong Gu","doi":"10.1016/j.cbpa.2025.111884","DOIUrl":"10.1016/j.cbpa.2025.111884","url":null,"abstract":"<div><div>Activation of extracellular signal-regulated kinase (ERK) signaling is related to chilling-induced diapause termination in <em>Bombyx mori</em> eggs. However, the changes in expressions of upstream signaling and downstream target genes are not very clear. In the present study, I investigated temporal changes in gene expressions of the prothoracicotropic hormone (PTTH) and epidermal growth factor (Egf) signaling pathways, which are the major stimulators of ERK signaling in insect systems, during a long chilling period. My results showed that when chilling was conducted from 2 days post-oviposition, expressions of PTTH/Egf signaling pathway genes were maintained at relatively higher levels, compared to those kept at 25 °C. In eggs exposed to 5 °C from 15 days post-oviposition, expression of the <em>PTTH</em> gene had dramatically increased by day 45 after chilling. Egf signaling pathway genes, including <em>spitz</em> (<em>spi</em>), <em>vein</em> (<em>vn</em>), <em>rhomboid</em> (<em>rho</em>), and <em>Egf receptor</em> (<em>Egfr</em>), showed gene-specific increasing patterns during the chilling period. I further showed that expression of the <em>pointed</em> (an ERK target) gene was maintained at a relatively higher when chilling was conducted from 2 days post-oviposition. Expression of the <em>pointed</em> gene had dramatically increased by day 45 in eggs chilled beginning after 15 days post-oviposition. Additionally, dechorionation increased <em>pointed</em> gene expression. LY294002 (a specific phosphatidylinositol 3-kinase (PI3K) inhibitor) and U0126 (an ERK inhibitor) inhibited <em>pointed</em> gene expression in dechorionated eggs, indicating that both PI3K and ERK are involved in increased <em>pointed</em> gene expression. When eggs that had been kept at 5 °C for 30 days were transferred to 25 °C, expression levels of <em>PTTH</em> and <em>pointed</em> genes decreased after transfer, clearly indicating that continuous chilling for 45 days is necessary to sustain the higher expressions of these genes. To the best of my knowledge, this study presents the first comprehensive analysis of the transcriptional regulation of both upstream and downstream genes in the ERK signaling pathway during a chilling period, offering valuable insights into the mechanisms underlying chilling-induced diapause termination.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111884"},"PeriodicalIF":2.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic changes associated with live transport stress of Haliotis rubra abalone","authors":"Nguyen Van Thao ,&nbsp;Jaime McAllister ,&nbsp;Andrea C. Alfaro ,&nbsp;Nha T. Ngo ,&nbsp;Craig Mundy","doi":"10.1016/j.cbpa.2025.111883","DOIUrl":"10.1016/j.cbpa.2025.111883","url":null,"abstract":"<div><div>Transport stress significantly influences the well-being and survival of abalone, yet the underlying metabolic changes and physiological responses associated with this stress remain poorly understood. To gain comprehensive insights into the metabolic changes and physiological responses of abalone (<em>Haliotis rubra</em>) under transport stress, we conducted a targeted liquid chromatography–mass spectrometry (LC–MS) metabolomics investigation on haemolymph and gill samples collected at different time points, including post-harvesting (pre-transport), post-transport and post-immersion (water holding period) and post-live exporting transport. The results revealed 143 and 141 metabolites that were significantly different among sampling times in haemolymph and gill, respectively. Notably, most of the metabolite differences occurred between the post-transport and post-immersion times, which indicate the strong impacts of transport stress on abalone metabolism. Interestingly, certain metabolites, such as lactic acid, succinic acid, L-hydroxyglutaric acid, uric acid, and myo-inositol, showed time-dependent increases during transport, suggesting their potential as stress biomarkers in abalone. Moreover, abalone that were acclimatized in holding tanks exhibited lesser metabolic changes compared to non-acclimatized, despite both groups being transported with oxygen supply. This highlights the significance of acclimatization and oxygen supply in reducing stress for abalone during transport. Enrichment analysis on abalone samples at 24 h post-transport compared to 96 h post-immersion identified 12 significantly impacted pathways in haemolymph and 34 pathways in gill tissues, indicating a range of metabolic disturbances in transported abalone, such as energy-related pathways, amino acid metabolisms, carbohydrate metabolisms, vitamin metabolisms, oxidative stress, and others. These results offer valuable insights into the physiological responses of abalone to transport stress, guiding improved transport practices to ensure good quality of products arriving at their market destinations.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111883"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacology reveals neuropeptide Y as a novel differential regulator of adrenal steroids in the globally invasive red-eared slider turtle (Trachemys scripta elegans)","authors":"Yoke Tassent,&nbsp;H. Bobby Fokidis","doi":"10.1016/j.cbpa.2025.111882","DOIUrl":"10.1016/j.cbpa.2025.111882","url":null,"abstract":"<div><div>The regulation of adrenal steroid secretion during acute stress has been poorly explored in reptiles. Using pharmacological manipulations of adrenocorticotropic hormone (ACTH), dexamethasone (DEX), neuropeptide Y (NPY), and NPY receptor antagonists, we examined glucocorticoid and DHEA responses to identify pathways potentially linking stress adaptation and energy homeostasis in the red-eared slider (<em>Trachemys scripta elegans</em>). Acute handling stress increased corticosterone concentrations as did injection with ACTH, but NPY and receptor antagonist treatments prevented this endogenous increase in corticosterone. In contrast, cortisol, and DHEA were not impacted by handling stress or ACTH, but both steroids increased in response to injection with NPY and with an established Y2 receptor antagonist. Together these data reveal the possibility that both ACTH and NPY act to regulate adrenal steroid synthesis, through a complicated and currently poorly understood mechanism. As red-eared sliders are highly invasive on a global scale, these insights can enhance our understanding of reptilian stress physiology to inform management strategies as well as bridge the gap between fundamental biology and applied conservation efforts, demonstrating the broader value of integrative research in addressing ecological and biomedical challenges.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111882"},"PeriodicalIF":2.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}