Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"High temperature induces the upward shift of the thermal neutral zone and decreases metabolic capacity in zebra finches.","authors":"Jing Wang, Ming-Hui Shao, Juan Li, Jia-Qi Liu, Xin-Yi Xu, Ming-Ru Xu, Ming Li, Jin-Song Liu","doi":"10.1016/j.cbpa.2024.111788","DOIUrl":"10.1016/j.cbpa.2024.111788","url":null,"abstract":"<p><p>The thermal neutral zone (TNZ) represents a fundamental concept in the thermal physiology of homeothermic organisms. TNZ is characterized as a specific range of environmental temperatures within which the metabolic rate remains at its basal level. The ambient temperature is regarded as a critical environmental factor that affects an animal's thermoregulation and propels the development of various morphological, physiological, and behavioral adaptations. In the present investigation, we assessed the influence of environmental temperature on various physiological parameters, including body mass, metabolic rate, thermoneutral zone (TNZ), state 4 respiration (S4R), cytochrome c oxidase (CCO) activity, body fat content, triglyceride content, free fatty acid content, β-hydroxyacyl Co-A dehydrogenase (HOAD) and citrate synthase (CS) activities, AMPK and PGC-1α mRNA levels, and triiodothyronine (T₃) and tetraiodothyronine (T₄) concentrations in zebra finches acclimated to 25 °C or 38 °C. zebra finches were found to have a TNZ of 32-42 °C when acclimated to 25 °C and a TNZ of 34-42 °C when acclimated to 38 °C. Acclimation to a high temperature led to an increase in the lower critical temperature (LCT), consequently resulting in a narrower TNZ. Zebra finches acclimated to 38 °C for a duration of four weeks exhibited a notable reduction in both body mass and basal metabolic rate as opposed to individuals maintained at 25 °C. Additionally, finches that were acclimatized to 38 °C exhibited a reduction in liver mass and a lower S4R level in both the liver and kidneys. Furthermore, these finches showed decreased CCO activity in the pectoral muscle and liver and lower avian uncoupling protein expression in the pectoral muscle compared with the birds acclimated to 25 °C. The T₃ level in the serum was lower in the 38 °C-acclimated finches than the 25 °C-acclimated finches. These findings suggested that the shift in the LCT of TNZ in zebra finches may possibly be associated with their metabolic capacity as well as their T₃ levels at a different ambient temperature. The changes in LCT of TNZ could be an important strategy in adapting to variations in ambient temperature in zebra finches.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111788"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does intestine length explain digesta retention times in birds and mammals?","authors":"María J Duque-Correa, Marcus Clauss, Carlo Meloro, Andrew J Abraham","doi":"10.1016/j.cbpa.2024.111789","DOIUrl":"10.1016/j.cbpa.2024.111789","url":null,"abstract":"<p><p>Previous studies have indicated that across birds and mammals, body mass is a comparatively poor predictor of the time digesta is retained in the digestive tract (mean retention time, MRT). Rather, MRT might be determined by gastrointestinal anatomy, which can differ considerably within and between trophic guilds. Here, we used two recent literature compilations on the intestine length and the MRT in birds (n = 33 species) and mammals (n = 149) and applied comparative statistical approaches to assess whether intestine length is more closely correlated with MRT than body mass. Regardless of the statistical model used, whether small species (< 120 g and any larger bats) were included or not, or whether birds and mammals were assessed together or separately, intestinal length generally yielded a better model fit to MRT than body mass, supporting the general concept. Nevertheless, data scatter was substantial, indicating that intestine length, though better than body mass, is still a limited explanatory factor for MRT. The intestine length-MRT relationship is an example of the direct statistical assessment of a generally presumed form-function relationship that is typically represented as a narrative. In the comparative literature, such assessments are rare.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111789"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of mitochondrial dynamics genes and mtDNA during embryonic development and under UVB exposure.","authors":"Thaline de Quadros, Michael Lorenz Jaramillo, Cairé Barreto, Rafael Diego da Rosa, Madson Silveira de Melo, Evelise Maria Nazari","doi":"10.1016/j.cbpa.2024.111790","DOIUrl":"10.1016/j.cbpa.2024.111790","url":null,"abstract":"<p><p>Studies using the embryos of the freshwater prawn Macrobrachium olfersii have reported changes in embryonic cells after exposure to ultraviolet B (UVB) radiation, such as DNA damage and apoptosis activation. Considering the importance of mitochondria in embryonic cells, this study aimed to characterize the aspects of mitochondrial morphofunctionality in M. olfersii embryos and mitochondrial responses to UVB radiation exposure. The coding sequences of genes Tfam, Nrf1, Mfn1, and Drp1 were identified from the transcriptome of M. olfersii embryos. The phylogenetic relationship showed strong amino acid identity and a highly conserved nature of the sequences. Additionally, the number of mitochondrial DNA (mtDNA) copies were higher in the early embryonic days. The results showed that the expression of the analyzed genes was highly regulated during embryonic development, increasing their levels near hatching. Furthermore, when embryos were exposed to UVB radiation, mitochondrial biogenesis was activated, recognized by higher levels of transcripts of genes Tfam and Nrf1, accompanied by mitochondrial fission. Additionally, these mitochondrial events were supported by an increase of mtDNA copies. Our results showed that UVB radiation was able to change the mitochondrial morphofunctionality, and under the current knowledge, certainly compromise embryonic cellular integrity. Additionally, mitochondria is an important cellular target of this radiation and its responses can be used to assess environmental stress caused by UVB radiation in embryos of aquatic species.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111790"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HSP70 is upregulated after heat but not freezing stress in the freeze-tolerant cricket Gryllus veletis.","authors":"Victoria E Adams, Maranda L van Oirschot, Jantina Toxopeus","doi":"10.1016/j.cbpa.2024.111791","DOIUrl":"10.1016/j.cbpa.2024.111791","url":null,"abstract":"<p><p>Heat shock proteins (HSPs) are well known to prevent and repair protein damage caused by various abiotic stressors, but their role in low temperature and freezing stress is not well-characterized in insects compared to other thermal challenges such as heat stress. Ice formation in and around cells is hypothesized to cause protein damage, yet many species of insects can survive freezing, suggesting HSPs may be an important mechanism in freeze tolerance. Here, we studied HSP70 in a freeze-tolerant cricket Gryllus veletis to better understand the role of HSPs in this phenomenon. We measured expression of one heat-inducible HSP70 isoform at the mRNA level (using RT-qPCR), as well as the relative abundance of total HSP70 protein (using semi-quantitative Western blotting), in five tissues from crickets exposed to a survivable heat treatment (2 h at 40 °C), a 6-week fall-like acclimation that induces freeze tolerance, and a survivable freezing treatment (1.5 h at -8 °C). While HSP70 expression was upregulated by heat at the mRNA or protein level in all tissues studied (fat body, Malphigian tubules, midgut, femur muscle, nervous system ganglia), no tissue exhibited HSP70 upregulation within 2-24 h following a survivable freezing stress. During fall-like acclimation to mild low temperatures, we only saw moderate upregulation of HSP70 at the protein level in muscle, and at the RNA level in fat body and nervous tissue. Although HSP70 is important for responding to a wide range of stressors, our work suggests that this chaperone may be less critical in the preparation for, and response to, moderate freezing stress.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111791"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic comparisons of jaw opening, jaw closing and locomotor muscles.","authors":"Clinton J Moran, Shannon P Gerry","doi":"10.1016/j.cbpa.2024.111782","DOIUrl":"10.1016/j.cbpa.2024.111782","url":null,"abstract":"<p><p>Understanding contraction dynamics of skeletal muscle is critically important to appreciate performance capabilities of skeletal structures, especially for structures responsible for feeding and/or locomotion. Furthermore, it is important to understand how temperature can impact contraction dynamics in vertebrates that are regularly exposed to fluctuations in temperature. We aimed to address differences between jaw opening (sternhyoideus), jaw closing (adductor mandibulae) and locomotor (abductor superficialis) muscle contraction dynamics in a labrid fish. Additionally, we aimed to understand how temperature changes impact contraction kinetics in these muscles. To address these aims we collected cunner (Tautogolabrus adspersus) from Long Island Sound and removed their primary locomotor and jaw opening/closing muscles. Following dissection, the muscles were placed on a muscle ergometer that controlled length, stimulus duration and stimulation intensity. Muscles were exposed to 7, 15 and 22 °C for all experiments. We found that the swimming muscle was slower to contract and relax than both feeding muscles. Swimming muscle produced more power than feeding muscles when tested at 8 Hz and 15 °C. Jaw closing muscle produced more force than the other two muscle types when tested at22 °C. Despite these differences, muscle contraction kinetics were remarkably similar across the three muscle types and temperatures. To our knowledge this is the first study to measure in vitro contraction dynamics of fish jaw opening and closing muscle. This advances the understanding of the physiological capabilities of these muscles. Additionally, differences in contraction dynamics can further our understanding of the physiological limits temperature impart on whole muscle contraction.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111782"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolactin locally mediates follicular atresia in hyperprolactinemic vizcachas (Rodentia, Chinchillidae).","authors":"Santiago A Cortasa, Luisa A Quiroga-Baeza, Alejandro R Schmidt, Sofía Proietto, Maria C Corso, Alfredo D Vitullo, Verónica B Dorfman, Julia Halperin","doi":"10.1016/j.cbpa.2024.111787","DOIUrl":"10.1016/j.cbpa.2024.111787","url":null,"abstract":"<p><p>Infertility in hyperprolactinemic females is attributed to the dysregulation of GnRH release, subsequently affecting gonadotropin levels, and ultimately leading to anovulation. However, in addition to the hypothalamus, prolactin receptor (PRLR) is expressed in ovaries as well, suggesting potential local effects of PRL in cases of hyperprolactinemia. We have developed an experimental model of sulpiride (SPD)-induced hyperprolactinemia using a wild rodent, the plains vizcacha, and studied the implications of pharmacological PRL levels on folliculogenesis and steroid production. Ovaries of SPD females showed a striking number of atretic follicles along with a reduction in the collective number of viable follicles leading to a higher atretic/viable follicle ratio compared to that of control females (CTL) (P < 0.05). In terms of sensitivity to the hormonal environment, SPD ovaries substantially changed their potential responsiveness to pituitary PRL, as evidenced by the three-fold increase in PRLR expression alongside heightened expression of both gonadotropic receptors in comparison to CTL ovaries (P < 0.05). Circulating estradiol (E2) values doubled post-treatment in the SPD females, which also showed higher expressions of aromatase and 17β-hydroxysteroid dehydrogenase, along with both E2 receptors, ERα and ERβ than the CTL group (P < 0.05). Our findings strongly suggest that hyperprolactinemia-dependent dysregulation of ovarian function can be explained at least partially, by PRL direct actions facilitated by the heightened expression of PRLR in follicles and corpora lutea. Possibly these PRL actions synergize with those triggered by gonadotropic hormones ultimately leading to alteration of the steroidogenic pathway, folliculogenesis disruption and increased atresia.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111787"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of severity and region of gill tissue resection on regeneration in Atlantic salmon (Salmo salar).","authors":"Ensiyeh Ghanizadeh-Kazerouni, Dean J Yoo, Simon R M Jones, Colin J Brauner","doi":"10.1016/j.cbpa.2025.111815","DOIUrl":"https://doi.org/10.1016/j.cbpa.2025.111815","url":null,"abstract":"<p><p>In a previous study, we demonstrated successful regeneration of Atlantic salmon gill tissue following up to 50 % filament resection. The present study explored 1) the capacity of gill tissue to regenerate following more severe trauma, 2) if regeneration potential varies across regions of the arch, and 3) how tissue loss impacts the physiology of neighboring unresected filaments. Fish were divided between two resected groups and a control non-resected one. In resection group-1, fish underwent 50 % and 75 % resection in the ventral and medial-dorsal regions of the first arch, while in resection group-2, the location of resection levels were reversed. The degree of filament regeneration and physiology of unresected filaments were measured at 4, 12 and 20 weeks-post-resection (WPR). Overall, the degree of regeneration was significantly higher in 50 % resected filaments relative to 75 % resected filaments. The degree of regeneration did not differ significantly between the resected groups for either of resection levels, suggesting negligible impacts of filament location on arch on regeneration. The concentration of oxidized glutathione (GSSG), total glutathione (GSH), and citrate synthase activity (CSA) in intact filaments were comparable between resected and control fish at both 4 and 20 WPR. However, GSH concentration varied among resected fish with those exhibited higher GSH in intact filaments showed lower regeneration of 50 % resected filaments at 20 WPR. Our results indicate that gill tissue loss exceeding 50 % may significantly impair regeneration and that this level of tissue loss is not associated with a compensatory response (e.g. GSSG, GSH, CSA) of neighboring gill tissue.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111815"},"PeriodicalIF":2.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and lipid metabolic differences between migratory and Egyptian-domesticated Mallard ducks (Anas platyrhynchos).","authors":"Basma Sheta, Asmaa Hassan, Alaa El-Din Sallam, Lotfy Habbak, Ayman Hyder","doi":"10.1016/j.cbpa.2025.111814","DOIUrl":"https://doi.org/10.1016/j.cbpa.2025.111814","url":null,"abstract":"<p><p>Although a giant Egyptian domestic non-migratory duck breed is phenotypically identical to the migratory Mallard, yet it is three times larger. The current study sought to determine the genetic and metabolic differences between this duck and Mallard, which arrives in Egypt in September for wintering and departs in March. Mitochondrial DNA control region (D-loop) was extracted, amplified, sequenced, and analyzed in both ducks. Both ducks were given a high-fat diet (HFD) for 6 weeks to assess their metabolic response to this diet. Polymorphism results indicated that the D-loop is highly variable and both populations expansion is balanced. The hierarchical analysis of molecular variants (AMOVA) and interpopulation difference parameters revealed significant genetic differentiation and minimal gene flow between migrant and resident populations. Phylogeny and Network analyses revealed that domestic ducks are a distinct group that separated from mallards. Physiologically, domestic duck blood and adipose tissue had a higher level of triglycerides and adipocyte volume than that of the depleting arriving migratory Mallard ducks, while leaving Mallard parameters were the highest, suggesting a high level of preparatory fat deposition and utilization before starting the trip. In response to HFD, the expression of FA uptake genes cd36, fabp1 was upregulated similarly in livers of domestic and migratory Mallard ducks, while the expression of lipid accumulation genes dgat2 and plin2 was higher in domestic than in migratory Mallards. However, the highest body mass and adipocytes volume gain was observed in the arriving migratory Mallards. In pectoral muscle, the expression of cd36 and fabp3 was higher in domestic than in leaving ducks, while in arriving Mallards, both genes were not upregulated in response to HFD. Dgat2 was upregulated only in domestic muscle, while lipid oxidation genes cpt1, lpl, and the controlling ppara were more upregulated in leaving Mallard. In conclusion, both ducks can be genetically and metabolically differentiated. Migratory mallards are more flexible and efficient in lipid metabolism than domestic ducks.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111814"},"PeriodicalIF":2.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food intake and the HPT axis in the cichlid fish: The implications of the gut-brain peptide cholecystokinin.","authors":"Mallikarjun Gouda, C B Ganesh","doi":"10.1016/j.cbpa.2025.111813","DOIUrl":"https://doi.org/10.1016/j.cbpa.2025.111813","url":null,"abstract":"<p><p>This work aimed to investigate the response of cholecystokinin (CCK) to starvation and its impact on food intake and the reproductive axis of the tilapia Oreochromis mossambicus. The fish subjected to 21 days of starvation showed a significant decrease in CCK immunoreactivity in the hypothalamus, pituitary gland, and intestine. The administration of injections of 0.5 and 5 μg of sulfated CCK-8 (CCK-8S) over 21 days resulted in a significant, dose-dependent decrease in food consumption. Administration of a high dose of CCK-8S (5 μg) resulted in a substantial decrease in the number of various spermatogenic cells, the intensity of androgen receptor immunoreactivity in the testis, and the percentage area of GnRH-immunoreactive fibres in the pars distalis of the pituitary gland (PPD), concurrent with a significant decline in serum levels of luteinizing hormone (LH) and 11-ketotestosterone (11-KT). Moreover, CCK-8S treatment markedly reduced the in vitro testicular 11-KT level. The results indicate for the first time that CCK influences hypothalamic GnRH release into the pituitary gland, resulting in the inhibition of LH release from the pituitary and affecting testicular spermatogenesis, androgen receptor protein expression, and steroidogenesis in teleosts. In addition, CCK may also directly affect testicular steroidogenesis.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111813"},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of olfactory-related neuropeptide transcripts in male Macrobrachium rosenbergii in correlation to pheromone perception from molting females.","authors":"Thanapong Kruangkum, Kornchanok Jaiboon, Rapeepun Vanichviriyakit, Prasert Sobhon, Charoonroj Chotwiwatthanakun","doi":"10.1016/j.cbpa.2025.111812","DOIUrl":"10.1016/j.cbpa.2025.111812","url":null,"abstract":"<p><p>Our previous studies revealed a mating attractant or possibly a pheromone released from molting reproductive mature female prawns, Macrobrachium rosenbergii, stimulates the expression of insulin-like androgenic gland hormones in a co-culture system. The released attractant is perceived by olfactory receptors with setae located on the short lateral antennules (slAn), which connect to the olfactory neuropil in the central nervous system (CNS) of male prawns. This neural signaling propagating through the CNS is mediated by at least four neuropeptides, namely neuropeptide F (NPF), short NPF (sNPF), tachykinin (TK), and allatostatin-A (ATS-A) whose transcripts have been detected in the present study. These deduced sequences, along with their conserved domains, serve as signatures of the identified neuropeptides, which were then compared with those found in other crustaceans and insects, whose nucleotide sequences were obtained from the nucleotide database. RT-PCR identified the expressions of the transcripts encoding these neuropeptides in the CNS. In situ hybridization specifically localized these transcripts in olfactory-associated neurons of cluster 9/11 of the deutocerebrum. Quantitative real-time PCR was used to quantify the expressions of the transcripts in response to the female attractants under different co-culture conditions: males with molting females (G1), males with intermolt females (G2), and slAn ablated males with molting females (G3). The transcripts were significantly increased on days 4-8 in the brain (Br) of males in G1 but not in G2 and G3. This suggests that expressions of the transcripts encoding the neuropeptides are associated with the perception of female mating pheromones through the slAn. This study is the first to show that female mating chemicals regulate the expressions and abundance of the olfactory neuropeptides, thus providing valuable insights for manipulation of mating of this species in aquaculture production.</p>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":" ","pages":"111812"},"PeriodicalIF":2.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}