Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Functional enzymatic characterisation and microbiome analysis of the digestive tract of the green sea urchin Strongylocentrotus droebachiensis","authors":"Marie Koch ,&nbsp;Sylke Wohlrab ,&nbsp;Reinhard Saborowski","doi":"10.1016/j.cbpa.2025.111907","DOIUrl":"10.1016/j.cbpa.2025.111907","url":null,"abstract":"<div><div>The digestive system of sea urchins is well-described, but the biochemical properties of the individual organs are only insufficiently characterized. The digestive tract begins in the buccal cavity, surrounded by a biting apparatus called Aristoteles' lantern. It then forms a tubular structure, which runs two times around the body wall, ending at the aboral side of the animal. This main part of the digestive tube has been described histologically as the stomach (first loop, anticlockwise) and the intestine (second loop, clockwise). We characterized the enzymatic profile as well as the microbial composition of the stomach and the intestine of the green sea urchin <em>Strongylocentrotus droebachiensis</em>, an ecological and economical important arctic-boreal grazer. Using qualitative and quantitative enzyme activity assays on tissue of the stomach and intestine, we identified differences in the biochemical processes, primarily concerning the protein and lipid metabolism. The stomach expressed higher esterase and esterase/lipase activity, while the intestine showed predominantly high exopeptidase activity. Low carbohydrate degrading enzyme activity suggests that polysaccharide digestion, despite their abundance in the species' diet, may primarily occur extracellularly in the lumen of the digestive tube. Our enzymatic findings are complimented by metabarcoding of the microbial community and analysis of functional properties: We found microbial taxa possibly associated with nitrogen fixation and carbohydrate degradation. Nevertheless, the composition and potential functional genes of the microbial community were similar between the two organs, suggesting that they do not play an organ-specific role in digestive processes.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"308 ","pages":"Article 111907"},"PeriodicalIF":2.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival and acute osmoregulatory response of grass carp under salinity stress","authors":"Zhu Zhu ,&nbsp;Shengjie Li ,&nbsp;Caixia Lei ,&nbsp;Tao Zhu ,&nbsp;Jing Tian ,&nbsp;Jinxing Du ,&nbsp;Shina Wei ,&nbsp;Hongmei Song","doi":"10.1016/j.cbpa.2025.111905","DOIUrl":"10.1016/j.cbpa.2025.111905","url":null,"abstract":"<div><div>Inland saline waters, widely distributed globally, impose stringent constraints on aquaculture owing to their high osmotic pressure, preventing most aquatic organisms from surviving. As the most extensively farmed freshwater fish worldwide, the grass carp (<em>Ctenopharyngodon idella</em>) is confined to freshwater habitats. The adaptation of this species to saline aquaculture holds significant ecological and economic potential; however, the mechanisms underlying its osmotic regulation in hypersaline environments remain poorly understood. In this study, juvenile grass carp with a mean body weight of 15.42 ± 0.96 g were subjected to acute salinity tolerance tests across six gradients (0, 4, 7, 10, 13, and 16 ppt). The 96 h median lethal salinity concentration (LC50) was first determined, followed by the evaluation of osmoregulatory dynamics through physiological-biochemical profiling and targeted quantification of salinity-responsive gene expression under acute stress conditions. Results showed mortality exhibited salinity-dependent escalation, and the LC50–96 h was 10.58 ppt. Under 24 h salinity exposure (0, 4, 7, and 10 ppt), grass carp exhibited salinity-dependent increases in serum electrolytes (Na⁺, Cl⁻, K⁺) and cortisol (<em>P</em> &lt; 0.05). Serum osmolality remained stable at 4 ppt and 7 ppt but increased significantly at 10 ppt. Serum prolactin showed progressive decline from 7 ppt. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the liver, kidney, and gills showed salinity-stimulated upregulation (significant at 7 and 10 ppt). Immune-related enzymes, acid phosphatase (ACP) and alkaline phosphatase (AKP), increased at 4 and 7 ppt, whereas AKP activity declined at 10 ppt. Gill Na⁺/K⁺-ATPase (NKA) activity was significantly elevated at 10 ppt (<em>P</em> &lt; 0.01). Moreover, both gill and kidney structures exhibited significant alterations under 7 ppt and 10 ppt salinity stress. Specifically, the gill showed cracks in the filaments, chloride cell hyperplasia, and detachment of flattened epithelial cells; renal tubules were atrophic. The expression of ion transport-related genes <em>NKA</em> and solute carrier family 12 member 2 (<em>SLC12A2</em>) in the gills increased with increasing salinity; both genes showed significant differences at 7 ppt and 10 ppt, indicating their role in regulating osmotic pressure balance. This study provides a theoretical basis for the saline aquaculture technology and salt-tolerant variety development in grass carp.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"308 ","pages":"Article 111905"},"PeriodicalIF":2.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia impairs survival and alters immune and iron metabolism gene expression during shark early ontogeny","authors":"Sandra Martins ,&nbsp;Jaquelino Varela ,&nbsp;Rute Felix ,&nbsp;Catarina Pereira Santos ,&nbsp;José Ricardo Paula ,&nbsp;Deborah M. Power ,&nbsp;Rui Rosa","doi":"10.1016/j.cbpa.2025.111904","DOIUrl":"10.1016/j.cbpa.2025.111904","url":null,"abstract":"<div><div>The global oxygen inventory has been declining worldwide, primarily due to climate change. The importance of oxygen for aerobic respiration and its homeostasis makes declining oxygen levels a concern, particularly during energy demanding lifecycle stages. The effects of low oxygen levels on neuroendocrine responses and immune competence of developing sharks were studied in the head and trunk tissues of early (EE; before pre-hatching) and late embryos (LE) of small-spotted catshark (<em>Scyliorhinus canicula</em>) under six days of deoxygenation (93 % O₂ of air saturation) and hypoxic conditions (26 % O₂). Catshark embryos were resilient to deoxygenation, with only a 10 % decline in survival compared to the control, and only the gene expression of melanotransferrin changed. Under hypoxia, growth was unaffected, but survival decreased by 31 % compared to the control in LE, highlighting an inadequate physiological response. Developmental stage affected the expression of hypoxia-inducible factor 1 alpha (<em>hif1a</em>), iron metabolism and immune-related genes, pointing to critical response mechanisms. The EE stage had an optimised stress response under hypoxia compared to LE, with the upregulation of the <em>hif1a</em> gene. The lack of a protective response and compromised immune-related functions under hypoxia in LE raise concerns about species survival under climate change. These findings highlight the need for further research on the likely resilience of sharks to environmental challenges.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"308 ","pages":"Article 111904"},"PeriodicalIF":2.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics-based study on metabolic-physiological response to heat stress in rainbow trout (Oncorhynchus mykiss)","authors":"Yang Yang ,&nbsp;Yingjie Liu ,&nbsp;Wenzhi Liu ,&nbsp;Lin Han ,&nbsp;Fangying Yuan ,&nbsp;Jing Wang ,&nbsp;Song Wu ,&nbsp;Yanchun Sun ,&nbsp;Shicheng Han","doi":"10.1016/j.cbpa.2025.111903","DOIUrl":"10.1016/j.cbpa.2025.111903","url":null,"abstract":"<div><div>Global warming poses a critical survival challenge for cold-water economic fish, necessitating elucidation of their physiological and metabolic responses to heat stress. This study established a rainbow trout heat stress model using a gradual temperature increase protocol (from 16 °C to 20 °C over 8 days, followed by maintenance at 20 °C for 22 days) and investigated the adverse effects and adaptive strategies of rainbow trout under heat stress. Extensive congestion and steatosis observed in liver tissues suggested that heat stress induced inflammatory responses and dysregulation of lipid metabolism. Significant increases in antioxidant enzyme activities and reactive oxygen species levels (1.29-fold) indicated activation of the antioxidant defense system, and this response was insufficient to alleviate oxidative damage. Integrative transcriptomic and metabolomic analyses revealed that lipid metabolism was the most significantly altered biological process. Elevated prostaglandin D2 (1.64-fold change), driven by disruption of arachidonic acid metabolism, triggered inflammatory responses. Adaptive upregulation of linoleic acid and α-linolenic acid metabolism likely maintained membrane fluidity, enhancing the capacity for heat stress adaptation. Concurrently, adaptive activation of both <em>GPX4A</em> (6.73-fold change) and the heat shock proteins (<em>LOC118938277</em> and <em>hsp47</em>) likely mitigated oxidative damage associated with heat stress in the liver. Overall, this study advances the understanding of pathological injuries and adaptive strategies in rainbow trout under heat stress and identifies candidate genes for breeding heat-tolerant strains.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"308 ","pages":"Article 111903"},"PeriodicalIF":2.1,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term exposure to dim light at night affects reproduction and metabolism-linked processes in adult male tree sparrows (Passer montanus)","authors":"Subu Yatung,&nbsp;Amit Kumar Trivedi","doi":"10.1016/j.cbpa.2025.111902","DOIUrl":"10.1016/j.cbpa.2025.111902","url":null,"abstract":"<div><div>Anthropogenic factors, particularly urbanization, have immense implications for the natural light-dark cycle of the species. The present study deliberates on the effects of dim light at night on the transcript expression of reproductive, steroidogenic, and metabolic gene markers in adult male tree sparrows (<em>Passer montanus</em>). Adult male birds were procured locally and were divided into two groups (<em>n</em> = 6 birds/group). Both groups were exposed to 12 h of light and 12 h of darkness, but group two (dLAN group) with a constant dim light (10 lx) during the dark hours. The experiment was run for 2 weeks. After that, birds were sampled, and the hypothalamus, liver, and gonads were harvested and used for gene expression analysis. Blood plasma was used for hormonal and biochemical assays. The findings suggest that 2 weeks of exposure did not significantly change the body mass, cholesterol, glucose, and testosterone assay. However, an increase in testicular volume was observed in dLAN-treated birds. Furthermore, elevation in hypothalamic transcripts (<em>Tshβ</em>, <em>Dio2</em>, <em>GnRH</em>, and <em>Eya3)</em> involved in the seasonal reproduction, along with an increase in steroidogenic transcripts (<em>StAr</em>, <em>Scp2</em>, <em>Srd5a1</em>, <em>Hsd11b2</em>, and <em>Er</em>) in the testis was observed; besides, liver metabolic transcript levels (<em>Acaca</em>, <em>Fasn</em>, <em>Hmcg</em>, <em>Idh2</em>, <em>Sdhaf4</em>, <em>Sdhaf2</em>, <em>Sdhc</em>, <em>Fh</em>, <em>Mdh</em>, <em>Foxo1</em>, and <em>Vip</em>) were also elevated in the dLAN-treated group compared to the control group. Overall, the study shows that even a short-term exposure to the lower intensity of dim light at night of 2 weeks of duration can stimulate the hypothalamic gonadal axis and liver metabolism in tree sparrows. These results could play a role in understanding the effect of light at night on the physiology of diurnal avian species.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111902"},"PeriodicalIF":2.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abnormalities in trehalose homeostasis reduce autophagy, antioxidant and response to air exposure stress in Eriocheir sinensis","authors":"Anjun Xiang ,&nbsp;Jiayi Chen ,&nbsp;Chengyi Weng ,&nbsp;Chenxu Liu ,&nbsp;Jie Zhang ,&nbsp;Qing He ,&nbsp;Yongxu Cheng ,&nbsp;Xiaozhen Yang","doi":"10.1016/j.cbpa.2025.111898","DOIUrl":"10.1016/j.cbpa.2025.111898","url":null,"abstract":"<div><div>Trehalose, a key carbohydrate in crustaceans and insects, is important in enhancing stress resistance. However, its regulatory mechanisms and functions in Chinese mitten crabs (<em>Eriocheir sinensis</em>) remain unclear. This study was designed to explore the regulation mechanisms of trehalose anabolism by synthesizing dsRNA interference fragments to target trehalose-6-phosphate synthase (<em>TPS</em>) and trehalose hydrolase (<em>TREH</em>) genes, investigating the effects of endogenous trehalose anabolism, antioxidant function, and autophagy in crabs under air exposure stress (AES). First, dsRNAs were synthesized, and interference fragments (EGFP, dsTPS1, and dsTREH2) and the optimal interference duration (48 h) were screened by in vitro culture. Further in vivo experiments demonstrated that interference with <em>TPS</em> and <em>TREH</em> genes led to abnormal trehalose anabolism in crabs subjected to AES; following interference, the expression of <em>TPS</em> gene was significantly down-regulated, while that of trehalose transporters (<em>TRET</em>) genes was significantly up-regulated; in the hepatopancreas, the levels of trehalose and glucose (Glu) were notably increased, whereas their levels in the hemolymph were significantly decreased. Abnormalities in trehalose homeostasis led to down-regulated expression of autophagy genes (<em>ATG4B</em>, <em>ATG7</em>, <em>ATG13</em>, <em>Beclin1</em>) and its activation pathway, MAPK signaling pathway genes (<em>ERK</em>, <em>JNK</em>, <em>p38</em>); this was accompanied by a decrease in p38 protein level, which ultimately led to decreased antioxidant capacity, increased vacuolated cells, and disturbed cellular arrangement in hepatopancreatic tissues of crabs under AES; there was an up-regulation of the expression of genes such as catalase (<em>CAT</em>), superoxide dismutase (<em>SOD</em>), and glutathione peroxidase (<em>GSH-PX</em>), which resulted in significant increase in the cumulative mortality rates.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"308 ","pages":"Article 111898"},"PeriodicalIF":2.1,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the IGFBP-rP cDNA and its associations with growth traits in Macrobrachium rosenbergii","authors":"Qianqian Ding,&nbsp;Yifan He,&nbsp;Weifeng Gao,&nbsp;Xiaojian Gao,&nbsp;Yao Zhang,&nbsp;Xiaojun Zhang,&nbsp;Qun Jiang","doi":"10.1016/j.cbpa.2025.111901","DOIUrl":"10.1016/j.cbpa.2025.111901","url":null,"abstract":"<div><div>Insulin-like growth factor binding proteins (IGFBPs) are key regulators of insulin-like growth factors (IGFs), which play essential roles in growth and development. In this study, a IGFBP related protein cDNA from <em>Macrobrachium rosenbergii</em> (designated <em>Mr-IGFBP-rP</em>) was identified. The full-length cDNA of <em>Mr-IGFBP-rP</em> is 1494 base pairs (bp) including a 22 bp 5′-untranslated region (UTR), a 707 bp 3’-UTR, and a 765 bp open reading frame (ORF) encoding a 254-amino acid protein. The Mr-IGFBP-rP protein contains three conserved domains: an IGF-binding domain (amino acids 34–89), a Kazal-type serine protease inhibitor domain (102–125), and an immunoglobulin-like C2-type domain (147–245). Sequence analysis revealed that Mr-IGFBP-rP shares 75.74 % identity with the IGFBP-rP1 of <em>Palaemon carinicauda</em> and 63.26 % with that of <em>Penaeus chinensis</em>. Expression analysis showed that <em>Mr-IGFBP-rP</em> is most highly expressed in the abdominal ganglia, followed by gill, heart, and brain. Eleven single nucleotide polymorphisms (SNPs) were identified within <em>Mr-IGFBP-rP</em>. Of these, three SNPs showed significant associations with growth traits in <em>M. rosenbergii</em>. Specifically, individuals with the AA genotype at the 5263-G/A locus exhibited greater body weight. Those with the TT genotype at the 5302-G/T locus showed increased body weight, cephalothorax height, and abdominal segment 1 height. In contrast, the CC genotype at the 5743-G/C locus was associated with reduced cephalothorax length, cephalothorax height, abdominal segment 1 height, and abdominal length. This study lays a foundation for understanding the role of <em>Mr-IGFBP-rP</em> in growth regulation in <em>M. rosenbergii</em>. The identified SNPs hold potential as candidate markers for marker-assisted selection in this species.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111901"},"PeriodicalIF":2.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water pH alters acid-base compensatory responses in white sturgeon (Acipenser transmontanus) exposed to modest and severe environmental hypercapnia","authors":"Ryan B. Shartau ,&nbsp;Daniel W. Baker","doi":"10.1016/j.cbpa.2025.111900","DOIUrl":"10.1016/j.cbpa.2025.111900","url":null,"abstract":"<div><div>Elevated CO₂ (hypercapnia) in fishes induces a respiratory acidosis, which is compensated by branchial acid-base relevant ion transfer. This organismal compensation is hypothesized to be affected by water pH, but assessing this effect is difficult when examining fish species (e.g., rainbow trout <em>Oncorhynchus mykiss</em>, Japanese flounder, <em>Paralichthys olivaceus</em>) that rely on extracellular pH regulation (pHe) to drive recovery as when it fails, mortality may occur quickly. Examining fishes that both perform pHe compensation, but do not require it for survival due to preferential intracellular pH (pH_i) regulation (PPR) such as does the white sturgeon (<em>Acipenser transmontanus</em>), allows us to determine the effect of water pH on acid-base compensation during hypercapnia. White sturgeon were exposed to 48 h of elevated CO₂ tensions while manipulating water pH to determine how water pH can alter patterns of acid-base compensation. Our findings demonstrate that water pH manipulation can have significant effects on the pHe compensatory response. For example, complete pHe compensation was observed in fish exposed to water equilibrated with 1 kPa CO₂, but reducing water pH by ∼1 pH unit prevented complete compensation. Alternately, when sturgeon were exposed to alkalized water equilibrated with 4 kPa CO₂ (i.e., pH of 6.3 compared to 5.7 for water with 4 kPa CO₂), pH compensation was more rapid. Water pH and pHe compensation rates had no effect on pH_i regulation. Our findings demonstrate that water pH can aid or assist pHe compensatory rates at a range of hypercapnic challenges, and so may have contributed to the origin of PPR as a primary strategy for some fish species to protect critical tissues largely independent of environmental conditions.</div></div><div><h3>Summary</h3><div>White sturgeon were exposed to two levels of elevated CO₂ (1 and 4 kPa) in water of two different pH values, and we tracked pH compensatory responses over the following two days. Acidifying water at modest CO₂ levels within the limit for complete compensation reduced the rate at which blood pH recovered. Alkalizing water during severe hypercapnia typically beyond that limit increased both the magnitude and the rate of organismal pH compensation. Intracellular pH protection was unaffected by any treatment.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111900"},"PeriodicalIF":2.1,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of individual differences in anxiety on anti-predator responses, thermal and hypoxia tolerance in zebrafish (Danio rerio)","authors":"Xiao-hong Li,&nbsp;Cheng Fu,&nbsp;Xue-ting Tan,&nbsp;Shi-jian Fu","doi":"10.1016/j.cbpa.2025.111899","DOIUrl":"10.1016/j.cbpa.2025.111899","url":null,"abstract":"<div><div>Anxiety, as a crucial stress response to potential threats in animals, exhibits significant individual differences, with high-anxiety individuals typically allocating greater attentional resources to threat detection. We hypothesized that such individuals would demonstrate enhanced threat sensitivity and anti-predator responses, but compromised stress tolerance due to constrained energy allocation. Using the novel tank diving test, we screened zebrafish (<em>Danio rerio</em>) with high- and low-anxiety phenotypes and compared their anti-predator responses (anxiety-like behaviors, metabolic rates, and predator avoidance behaviors) under predator chemical or visual cues, along with their thermal and hypoxia tolerance capacities under baseline conditions. The results demonstrate that high-anxiety zebrafish exhibit enhanced anti-predator responses in threatening environments, while showing no compromised stress tolerance at baseline. These findings demonstrate the adaptive superiority of the high-anxiety phenotype in high-predation-risk environments, providing theoretical foundations for understanding the evolutionary role of anxiety in fish.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111899"},"PeriodicalIF":2.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Temporal changes in PTTH/Egf signaling and ERK target gene expressions during chilling-induced diapause termination in Bombyx mori eggs” [Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Volume 307, September 2025, 111,884].","authors":"Shi-Hong Gu","doi":"10.1016/j.cbpa.2025.111895","DOIUrl":"10.1016/j.cbpa.2025.111895","url":null,"abstract":"","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"307 ","pages":"Article 111895"},"PeriodicalIF":2.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}