Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"The ontogeny of white leghorn chicken (Gallus domesticus) blood chemistry changes in response to acute exposure to 10 % O2","authors":"Dane A. Crossley II,&nbsp;James W. Hicks","doi":"10.1016/j.cbpa.2024.111736","DOIUrl":"10.1016/j.cbpa.2024.111736","url":null,"abstract":"<div><p>The embryonic chicken is a valuable model for studying the maturation of cardiovascular physiology and the responses of this organ system to environmental manipulations such as acute hypoxia. Hypoxia determines not only the general cardiovascular response but also is a tool to determine the system's maturation of reflexive control. Several studies suggest embryonic chicken's regulation of the cardiovascular response to hypoxia, but no studies have measured the blood chemistry changes that accompany these responses. To clarify the changes in blood parameters accompanying cardiovascular function changes during acute hypoxia, we designed a study to investigate the blood chemistry (pO₂, pCO₂, pH, lactate, glucose, and blood ions) in developing embryos during acute hypoxia (O₂ = 10 %). Embryos ranging from day 13 to 21 of incubation were sampled during a control period and at the end of a 5-min of hypoxia. Hypoxia caused bradycardia on all days of incubation. The maximal blood hypoxic response occurred on day 15, with lactate increasing 7-fold (2.5 to 16.6 mmol/l) while glucose levels decreased by 50 % (136 to 63 mg/dl). Furthermore, hypoxia reduced pH (7.40 to 7.26), which peaked on day 15. These data indicate that a 5-min exposure to 10 % O₂ is sufficient to induce dramatic changes in blood chemistry however chorioallantoic arterial blood pO₂ was unchanged on most days of the study. Therefore, given the cardiovascular response to hypoxia and the increase in blood lactate prior to airbreathing in the chicken embryo, the embryonic tissues experienced an acute stress that may be the basis for the change in cardiovascular function during the exposure.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"298 ","pages":"Article 111736"},"PeriodicalIF":2.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of oxidative status, detoxification capacity and immune responsiveness in honey bees with ageing","authors":"Jelena Spremo ,&nbsp;Jelena Purać ,&nbsp;Tatjana Čelić ,&nbsp;Srđana Đorđievski ,&nbsp;Ivan Pihler ,&nbsp;Danijela Kojić ,&nbsp;Elvira Vukašinović","doi":"10.1016/j.cbpa.2024.111735","DOIUrl":"10.1016/j.cbpa.2024.111735","url":null,"abstract":"<div><p>The honey bee (<em>Apis mellifera</em> L.), as an eusocial insect species, is an important model organism in research focusing on ageing and longevity, due to prominent seasonal lifespan plasticity within the worker caste (summer and winter worker bees). In this study, we employed a screening approach to evaluate several molecular parameters, providing comprehensive insights into the antioxidative (superoxide dismutase and catalase activity, reduced glutathione and sulfhydryl group content, total antioxidative capacity), detoxifying (glutathione <em>S</em>-transferase and acetylcholinesterase activity), and immune (phenol oxidase and glucose oxidase activity) status, as well as vitellogenin content, in the summer and winter generation of honey bees, across ageing stages and in two body compartments: the whole abdomen and the head. Summer worker bees were collected weekly for six weeks, while winter bees were collected monthly for five months. The results of our study clearly indicate a reduced overall antioxidative capacity of older groups of worker bees from both generations, while the parameters of immune responsiveness mostly contributed to the separation between the two generations based on season rather than age categories. Detoxification ability appeared to be more susceptible to environmental factors. An age-dependent increase in vitellogenin content was recorded in the abdomen, but without seasonal differences. These findings provide an excellent starting point for further investigations into age-related changes, particularly within the context of honey bee sociality.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"298 ","pages":"Article 111735"},"PeriodicalIF":2.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001624/pdfft?md5=369e7c638b385a71d168efb6cc49d070&pid=1-s2.0-S1095643324001624-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia-acclimation adjusts skeletal muscle anaerobic metabolism and burst swim performance in a marine fish","authors":"Benjamin Negrete Jr ,&nbsp;Kerri Lynn Ackerly ,&nbsp;Andrew J. Esbaugh","doi":"10.1016/j.cbpa.2024.111734","DOIUrl":"10.1016/j.cbpa.2024.111734","url":null,"abstract":"<div><p>Red drum, <em>Sciaenops ocellatus</em>, are a marine teleost native to the Gulf of Mexico that routinely experiences periods of low oxygen (hypoxia). Recent work has demonstrated this species has the capacity to improve aerobic performance in hypoxia through respiratory acclimation. However, it remains unknown how hypoxia acclimation impacts anaerobic metabolism in red drum, and the consequences of exhaustive exercise and recovery. Juvenile fish were acclimated to normoxia (<em>n</em> = 15, DO 90.4 ± 6.42 %) or hypoxia (n = 15, DO 33.6 ± 7.2 %) for 8 days then sampled at three time points: at rest, after exercise, and after a 3 h recovery period. The resting time point was used to characterize the acclimated phenotype, while the remaining time points demonstrate how this phenotype responds to exhaustive exercise. Whole blood, red muscle, white muscle, and heart tissues were sampled for metabolites and enzyme activity. The resting phenotype was characterized by lower pH_e and changes to skeletal muscle ATP. Exhaustive exercise increased muscle lactate, and decreased phosphocreatine and ATP with no effect of acclimation. Interestingly, hypoxia-acclimated fish had higher pH_e and pH_i than control in all exercise time points. Red muscle ATP was lower in hypoxia-acclimated fish versus control at each sample period. Moreover, acclimated fish increased lactate dehydrogenase activity in the red muscle. Hypoxia acclimation increased white muscle ATP and hexokinase activity, a glycolytic enzyme. In a gait-transition swim test, hypoxia-acclimated fish recruited anaerobic-powered burst swimming at lower speeds in normoxia compared to control fish. These data suggest that acclimation increases reliance on anaerobic metabolism, and does not benefit recovery from exhaustive exercise.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111734"},"PeriodicalIF":2.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001612/pdfft?md5=ac010c18efcaf63bfa338f59a9c706db&pid=1-s2.0-S1095643324001612-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature effects on blood gases in embryonic American alligators (Alligator mississippiensis)","authors":"Dane A. Crossley II ,&nbsp;Janna L. Crossley ,&nbsp;Justin L. Conner ,&nbsp;Brandt Smith ,&nbsp;Ruth Elsey ,&nbsp;Derek Nelson ,&nbsp;Tobias Wang","doi":"10.1016/j.cbpa.2024.111733","DOIUrl":"10.1016/j.cbpa.2024.111733","url":null,"abstract":"<div><p>Numerous studies report on the influence of temperature on blood gases in ectothermic vertebrates, but there is merely a cursory understanding of these effects in developing animals. Animals that develop in eggs are at the mercy of environmental temperature and are expected to lack the capacity to regulate gas exchange and may regulate blood gases by means of altered conductance for gas exchange. We, therefore, devised a series of studies to characterize the developmental changes in blood gases when embryonic alligators were exposed to 25, 30 and 35 °C. To determine how blood parameters were impacted by changes in embryonic temperature, blood was sampled from the chorioallantoic membrane artery. The blood in the chorioallantoic membrane artery is a mixture of oxygen-poor and oxygen-rich blood, which based on the embryonic vascular anatomy may reflect blood that perfuses the chemoreceptors of the developing animal. Our findings indicate that following a 48 h exposure to 25 °C or 35 °C, there was a positive relationship between CAM artery blood PO₂, PCO₂ and glucose. However, blood pH suggests embryonic alligators lack an acute regulatory mechanism for adjusting blood pH.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111733"},"PeriodicalIF":2.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistent interactive effects of developmental salinity and temperature in Atlantic killifish (Fundulus heteroclitus)","authors":"Madison L. Earhart ,&nbsp;Myra Thapar ,&nbsp;Tessa S. Blanchard ,&nbsp;William S. Bugg ,&nbsp;Patricia M. Schulte","doi":"10.1016/j.cbpa.2024.111732","DOIUrl":"10.1016/j.cbpa.2024.111732","url":null,"abstract":"<div><p>Climate change alters multiple abiotic environmental factors in aquatic environments but relatively little is known about their interacting impacts, particularly in developing organisms where these exposures have the potential to cause long-lasting effects. To explore these issues, we exposed developing killifish embryos (<em>Fundulus heteroclitus</em>) to 26 °C or 20 °C and 20 ppt or 3 ppt salinity in a fully-factorial design. After hatching, fish were transferred to common conditions of 20 °C and 20 ppt to assess the potential for persistent developmental plasticity. Warm temperature increased hatching success and decreased hatch time, whereas low salinity negatively affected hatching success, but this was only significant in fish developed at 20 °C. Temperature, salinity, or their interaction affected mRNA levels of genes typically associated with thermal and hypoxia tolerance (<em>hif1a, hsp90b, hsp90a, hsc70,</em> and <em>hsp70.2</em>) across multiple developmental timepoints. These differences were persistent into the juvenile stage, where the fish that developed at 26 °C had higher expression of <em>hif1a, hsp90b, hsp90a,</em> and <em>hsp70.2</em> than fish developed at 20 °C, and this was particularly evident for the group developed at both high temperature and salinity. There were also long-lasting effects of developmental treatments on body size after four months of rearing under common conditions. Fish developed at low salinity or temperature were larger than fish developed at high temperature or salinity, but there was no interaction between the two factors. These data highlight the complex nature of the developmental effects of interacting stressors which has important implications for predicting the resilience of fishes in the context of climate change.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111732"},"PeriodicalIF":2.1,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001594/pdfft?md5=69c6309ef36f01d0d137820ea5c1a53f&pid=1-s2.0-S1095643324001594-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: An alternative pathway for melatonin synthesis in the skin of European flounder (Platichthys flesus)","authors":"Magdalena Gozdowska ,&nbsp;Joanna Stoń-Egiert ,&nbsp;Ewa Kulczykowska","doi":"10.1016/j.cbpa.2024.111731","DOIUrl":"10.1016/j.cbpa.2024.111731","url":null,"abstract":"<div><p>The classic melatonin biosynthesis pathway (Mel; <em>N</em>-acetyl-5-methoxytryptamine) involves two consecutive enzymatic steps that are decisive in hormone production: conversion of serotonin (5-hydroxytryptamine; 5-HT) to <em>N</em>-acetylserotonin (NAS) and the methylation of the last compound to Mel. This pathway requires the activity of the enzymes: the first is of the category of <em>N</em>-acetyltransferases (AANAT, SNAT, or NAT) and the second is <em>N</em>-acetylserotonin <em>O</em>-methyltransferase (ASMT; also known as HIOMT). However, quite recently, new information has been provided on the possibility of an alternative Mel synthesis pathway; it would include a two-step action by these enzymes, but in reverse order, where ASMT (or ASMTL, the enzyme related to ASMT) methylates 5-HT to 5-methoxytryptamine (5-MT), and then the last compound is acetylated by an enzyme of the category of <em>N</em>-acetyltransferases to Mel. In our study on the activity of enzymes in the Mel biosynthesis pathway in flounder skin, we have found an increase in 5-MT level, as a result of the increase in 5-HT concentration, which is followed by a growing concentration of Mel. However, we have not found any increase in Mel concentration, despite an increase in NAS in the samples. Our data strongly suggest an alternative way of Mel production in flounder skin in which 5-HT is first methylated to 5-MT, which is then acetylated to Mel.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111731"},"PeriodicalIF":2.1,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001582/pdfft?md5=de7ed18ba362c8eb57e3503b0409ae25&pid=1-s2.0-S1095643324001582-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditions","authors":"Anamarija Žagar ,&nbsp;Urban Dajčman ,&nbsp;Rodrigo Megía-Palma ,&nbsp;Tatjana Simčič ,&nbsp;Frederico M. Barroso ,&nbsp;Senka Baškiera ,&nbsp;Miguel A. Carretero","doi":"10.1016/j.cbpa.2024.111729","DOIUrl":"10.1016/j.cbpa.2024.111729","url":null,"abstract":"<div><p>Aerobic respiration is the main energy source for most eukaryotes, and efficient mitochondrial energy transfer greatly influences organismal fitness. To survive environmental changes, cells have evolved to adjust their biochemistry. Thus, measuring energy metabolism at the subcellular level can enhance our understanding of individual performance, population dynamics, and species distribution ranges. We investigated three important metabolic traits at the subcellular level in five lacertid lizard species sampled from different elevations, from sea level up to 2000 m. We examined hemoglobin concentration, two markers of oxidative stress (catalase activity and carbonyl concentration) and maximum rate of metabolic respiration at the subcellular level (potential metabolic activity at the electron transport system). The traits were analysed in laboratory acclimated adult male lizards to investigate the adaptive metabolic responses to the variable environmental conditions at the local sampling sites. Potential metabolic activity at the cellular level was measured at four temperatures – 28 °C, 30 °C, 32 °C and 34 °C – covering the range of preferred body temperatures of the species studied. Hemoglobin content, carbonyl concentration and potential metabolic activity did not differ significantly among species. Interspecific differences were found in the catalase activity, Potential metabolic activity increased with temperature in parallel in all five species. The highest response of the metabolic rate with temperature (Q₁₀) and Arrhenius activation energy (E_a) was recorded in the high-mountain species <em>Iberolacerta monticola</em>.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111729"},"PeriodicalIF":2.1,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1095643324001569/pdfft?md5=491f3fca2d61ec57ba5674a2e3ed5346&pid=1-s2.0-S1095643324001569-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: Differential expression of piwi1 and piwi2 genes in tissues of tambacu and zebrafish: A possible relationship with the indeterminate muscle growth","authors":"Érika Stefani Perez ,&nbsp;Tassiana Gutierrez de Paula ,&nbsp;Bruna Tereza Thomazini Zanella ,&nbsp;Leonardo Nazário de Moraes ,&nbsp;Bruno Oliveira da Silva Duran ,&nbsp;Maeli Dal-Pai-Silva","doi":"10.1016/j.cbpa.2024.111730","DOIUrl":"10.1016/j.cbpa.2024.111730","url":null,"abstract":"<div><p>Fish skeletal muscle is a component of the human diet, and understanding the mechanisms that control muscle growth can contribute to improving production in this sector and benefits the human health. In this sense, fish such as tambacu can represent a valuable source for exploring muscle growth regulators due to the indeterminate muscle growth pattern. In this context, the genes responsible for the indeterminate and determinate muscle growth pattern of fish are little explored, with <em>piwi</em> genes being possible candidates involved with these growth patterns. Piwi genes are associated with the proliferation and self-renewal of germ cells, and there are descriptions of these same functions in somatic cells from different tissues. However, little is known about the function of these genes in fish somatic cells. Considering this, our objective was to analyze the expression pattern of <em>piwi</em> 1 and 2 genes in cardiac muscle, skeletal muscle, liver, and gonad of zebrafish (species with determinate growth) and tambacu (species with indeterminate growth). We observed a distinct expression of <em>piwi1</em> and <em>piwi2</em> between tambacu and zebrafish, with both genes more expressed in tambacu in all tissues evaluated. <em>Piwi</em> genes can represent potential candidates involved with indeterminate muscle growth control.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111730"},"PeriodicalIF":2.1,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coping with dry spells: Investigating oxidative balance and metabolic responses in the subtropical tree frog Boana pulchella (Hylidae) during dehydration and rehydration exposure","authors":"Marjoriane de Amaral ,&nbsp;Maiza Cristina Von Dentz ,&nbsp;Gustavo Kasper Cubas ,&nbsp;Diogo Reis de Oliveira ,&nbsp;Leonardo Airton Ressel Simões ,&nbsp;Jorge Felipe Argenta Model ,&nbsp;Guendalina Turcato Oliveira ,&nbsp;Luiz Carlos Kucharski","doi":"10.1016/j.cbpa.2024.111728","DOIUrl":"10.1016/j.cbpa.2024.111728","url":null,"abstract":"<div><p>In the face of climate change, understanding the metabolic responses of vulnerable animals to abiotic stressors like anurans is crucial. Water restriction and subsequent dehydration is a condition that can threaten populations and lead to species decline. This study examines metabolic variations in the subtropical frog <em>Boana pulchella</em> exposed to dehydration resulting in a 40% loss of body water followed by 24 h of rehydration. During dehydration, the scaled mass index decreases, and concentrations of metabolic substrates alter in the brain and liver. The activity of antioxidant enzymes increases in the muscle and heart, emphasizing the importance of catalase in the rehydration period. Glycogenesis increases in the muscle and liver, indicating a strategy to preserve tissue water through glycogen storage. These findings suggest that <em>B. pulchella</em> employs specific metabolic mechanisms to survive exposure to water restriction, highlighting tissue-specific variations in metabolic pathways and antioxidant defenses. These findings contribute to a deeper understanding of anuran adaptation to water stress and emphasize the importance of further research in other species to complement existing knowledge and provide physiological tools to conservation.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111728"},"PeriodicalIF":2.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different approaches to understanding methodological adequacy in ecophysiological studies on small ectotherms","authors":"Rafaella Silva Roseno ,&nbsp;Leildo Machado Carilo Filho ,&nbsp;Luisa Maria Diele-Viegas ,&nbsp;Bruno Teixeira de Carvalho ,&nbsp;Mirco Solé","doi":"10.1016/j.cbpa.2024.111726","DOIUrl":"10.1016/j.cbpa.2024.111726","url":null,"abstract":"<div><p>Body temperature (Tb) variation and environmental temperature gradients are more intense in small individuals because their body size allows for a more intimate relationship between Tb and the environment. To contribute to a methodological consensus on the ecophysiology of small ectotherms, we aimed to investigate whether different approaches and methodological techniques affect the measurement of critical temperatures in a small lizard (<em>Coleodactylus meridionalis, Sphaerodactylidae</em>) from the Atlantic Forest of southern Bahia, Brazil, and subsequently its vulnerability assessment. We measured two metrics of thermal physiology: critical thermal minimum (CTmin) and critical thermal maximum (CTmax). In total, four types of temperature measurements (protocols) were defined. In the first protocol, we estimated CTmax/CTmin without heating/cooling rate by directly measuring the lizard's midbody temperature. In the other three protocols, we used a ramping assay with a heating/cooling rate to estimate CTmax/CTmin in the chamber (height: 11.3 cm), substrate, and Tb of the lizard, respectively. In total 116 individuals of <em>Coleodactylus meridionalis</em> were collected, of which 177 CTmax and 131 CTmin were performed. <em>C. meridionalis</em> showed a mean CTmax of 41 °C and a mean CTmin of 8.9 °C when considering the Tb protocol, which is intermediate compared to the other protocols. The substrate temperature protocol was the closest to Tb, and for this, the best method for the small lizards using an infrared thermometer.</p></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"297 ","pages":"Article 111726"},"PeriodicalIF":2.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141914639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}