Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献

{"title":"Effects of air exposure time on tissue morphology, energy metabolism, and non-specific immunity of the juvenile Chinese mitten crab, Eriocheir sinensis","authors":"Aoxi Zhao ,&nbsp;Qinyuan Zhang ,&nbsp;Yunfei Sun ,&nbsp;Yongxu Cheng","doi":"10.1016/j.cbpb.2025.111090","DOIUrl":"10.1016/j.cbpb.2025.111090","url":null,"abstract":"<div><div>This study investigated the effect of aerial exposure duration (0, 24, 48, and 72 h) on the juvenile Chinese mitten crab, <em>Eriocheir sinensis</em>. The effects on histological structure, energy metabolism, non-specific immunity, and expression of cytochrome P450 (<em>CYP2</em>) and protein kinase B (<em>AKT</em>) genes in the anterior gill and hepatopancreas were analyzed. Longer durations of air exposure led to loss of anterior gill filament shape, and enlargement and deformation of the hepatopancreatic tubules with vacuolization. Pyruvate kinase activity in the hemolymph peaked, and was the lowest in the anterior gill, at 72 h. Lactate dehydrogenase activity and lactic acid content in the hemolymph and anterior gills peaked at 72 h of air exposure. Triglyceride content in hemolymph and anterior gill first increased, then decreased. Superoxide dismutase activity and total antioxidant capacity increased in the hemolymph but initially increased and then decreased in the hepatopancreas, peaking at 24 h. Hemolymph and hepatopancreas acid phosphatase activity peaked at 48 h and in these same tissues lysozyme activity declined with prolonged air exposure. The relative expression of <em>CYP2</em> and <em>AKT</em> increased during exposure to air in both gill and hepatopancreas. Air exposure exceeding 48 h may affect tissue morphology, energy metabolism, oxidative stress, and immunity of juvenile <em>E. sinensis</em>. Juvenile crabs alter their energy utilization, antioxidant, and metabolic capacities to acclimate to air exposure stress. Therefore, the air exposure time for juvenile <em>E. sinensis</em> should be limited to 48 h during transportation.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111090"},"PeriodicalIF":1.9,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purslane leaf powder dietary supplementation rescues cadmium-induced disruption of behavior, antioxidant status, and expression of tight junction genes, in the brain of Nile tilapia (Oreochromis niloticus)","authors":"Nawal Alsubaie ,&nbsp;Yasmina M. Abd-Elhakim ,&nbsp;Amany Abdel-Rahman Mohamed ,&nbsp;Rowida E. Ibrahim ,&nbsp;Mohamed M.M. Metwally ,&nbsp;Tarek Khamis ,&nbsp;Alaa S. Alhegaili ,&nbsp;Abd Elhakeem El-Murr ,&nbsp;Badriyah S. Alotaibi ,&nbsp;Ahmed Abdulwahab Bawahab","doi":"10.1016/j.cbpb.2025.111086","DOIUrl":"10.1016/j.cbpb.2025.111086","url":null,"abstract":"<div><div>This study examined the effects of supplementing the Nile tilapia (<em>Oreochromis niloticus)</em> diet with purslane (<em>Portulaca oleracea</em> L.) leaf powder (PLNP, 10 g/kg) on neurobehavioral performance, brain oxidative status, tight junction mRNA expression, and brain histology in fish exposed to waterborne cadmium (Cd, 50 μg /L) for 60 days. Adding PLNP to the diet ameliorated the Cd-induced decline in ingestive behavior and swimming behavior, and reversed the Cd-induced increase in aggressive behavior. The significant decrease in the non-enzymatic (reduced glutathione) and enzymatic (catalase and superoxide dismutase) brain antioxidants detected in Cd-exposed fish was eliminated by dietary PLNP. PLNP supplementation also led to a decrease in brain malondialdehyde content, which was elevated by Cd exposure. In addition, dietary PLNP increased brain acetylcholinesterase content, upregulated mRNA expression of tight junction (<em>zo-2</em>, <em>claudin-4</em>, and <em>zo-1</em>) and oxidative stress genes (<em>sod-2</em>, <em>gpx</em>, and <em>nrf-2</em>), and downregulated apoptotic genes (<em>p53</em>, <em>caspase-9</em>, <em>caspase-8</em>, and <em>caspase-3</em>) in the brain, relative to the alterations in these parameters caused by Cd exposure. Furthermore, the Cd-induced histological changes in the Nile tilapia brain were ameliorated by PLNP dietary supplementation. In light of these findings, PLNP may be a useful dietary supplement for reducing the harmful effects of Cd on the brain and behavior of Nile tilapia.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111086"},"PeriodicalIF":1.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Docosapentaenoic (22:5 n-6) and docosahexaenoic (22:6 n-3) acids exhibit highly lipogenic properties in rainbow trout preadipocytes","authors":"Gilles Tinant,&nbsp;Ineke Neefs,&nbsp;Alice De Groote,&nbsp;Melissa M. Page,&nbsp;Jean-François Rees,&nbsp;Yvan Larondelle,&nbsp;Cathy Debier","doi":"10.1016/j.cbpb.2025.111087","DOIUrl":"10.1016/j.cbpb.2025.111087","url":null,"abstract":"<div><div>Dietary polyunsaturated fatty acids are essential for fish health. Adipose tissue is the major tissue for fatty acid storage in rainbow trout (<em>Oncorhynchus mykiss</em>), and its development and function can be impacted by the fatty acids themselves. In the present study, the effects of seven fatty acids, oleic (OA, 18:1 n-9), α-linolenic (ALA, 18:3 n-3), eicosapentaenoic (EPA, 20:5 n-3), docosahexaenoic (DHA, 22:6 n-3), linoleic (LA, 18:2 n-6), arachidonic (AA, 20:4 n-6), and docosapentaenoic (DPA, 22:5 n-6) acids, on adipogenesis were investigated in primary cultures of rainbow trout preadipocytes. In terms of lipid accumulation, DPA and DHA appeared to be the most lipogenic fatty acids, while all treatments modified the fatty acid composition of the cellular phospholipids and neutral lipids. The fatty acid of interest added to the culture medium was the most abundant in preadipocytes, while the first bioconversion products were detected in lower amounts. In terms of transcriptional effects, DPA increased the expression of the early transcription factor CCAAT/enhancer binding protein δ, while DHA upregulated the expression of genes involved in neutral lipid synthesis, notably lipoprotein lipase, fatty acid transport protein 1 and glycerol-3-phosphate dehydrogenase. Both fatty acids decreased the expression of fatty acid synthase. These results highlight that DPA and DHA exert a significant effect on lipid deposition in rainbow trout preadipocytes, potentially through different pathways, and confirm that fatty acids have major impacts on preadipocyte lipid metabolism and adipogenesis.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111087"},"PeriodicalIF":1.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The heat shock cognate protein 70 (HSC70) gene in the echiuran worm Urechis unicinctus: Cloning, tissue expression and response to sulfide exposure","authors":"Long Zhu ,&nbsp;Sijie Wang ,&nbsp;Ziyan Zhao ,&nbsp;Baiyu Li ,&nbsp;Chenxiao Xi ,&nbsp;Xinghong Xu","doi":"10.1016/j.cbpb.2025.111077","DOIUrl":"10.1016/j.cbpb.2025.111077","url":null,"abstract":"<div><div>To investigate the molecular characteristics of the heat shock cognate protein 70 (<em>HSC70</em>) gene in the echiuran worm <em>Urechis unicinctus</em>, the full-length complementary DNA (cDNA) sequence of <em>HSC70</em> in <em>U. unicinctus</em> was cloned and characterized. Additionally, the expression of <em>HSC70</em> in different tissues of <em>U. unicinctus</em> was detected after exposure to 0.25, 0.75, and 1.25 mg L⁻¹ Na₂S for 3, 6, 12, 24, 48, and 72 h, respectively. Results showed that the full-length cDNA of the <em>HSC70</em> gene was 2305 bp and encoded a deduced 658-amino acid (aa) protein. Three characteristic structural features of the <em>HSP70</em> gene family: IVLVGGSTRIPKIQK (residues 334–348), IFDLGGGTFDVSV (residues 197–210), and IDLGTTYSCV (residues 9–18), were identified in the HSC70 amino acid sequence. Multiple sequence alignment showed that <em>HSC70</em> was 87.25 % identical to <em>Meretrix meretrix</em> at the amino acid level, and phylogenetic analyses suggested that the <em>HSC70</em> gene of <em>U. unicinctus</em> was most closely grouped with <em>Urechis caupo</em>. Additionally, <em>HSC70</em> was expressed in all tested tissues, with the highest expression in hemolymph. <em>HSC70</em> mRNA expression was significantly increased after sulfide exposure, reaching a peak 3–6 h into the exposure, suggesting that <em>HSC70</em> expression was sensitive to environmental factors. Na₂S exposure for 12 h significantly increased the muscle content of HSC70, while a significant decrease was observed after Na₂S exposure for 72 h. Taken together, our study findings suggest that the <em>HSC70</em> gene is rapidly induced in <em>U. unicinctus</em> in response to sulfide exposure.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111077"},"PeriodicalIF":1.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and functional analysis of a novel goose-type lysozyme from teleost Sebastes schlegelii with implications for antibacterial defense and immune cell modulation","authors":"Hao Jing ,&nbsp;Xue Yan ,&nbsp;Yue Wang ,&nbsp;Kai Yang ,&nbsp;Zi-yue Chen ,&nbsp;Guang-Hua Wang ,&nbsp;Min Zhang","doi":"10.1016/j.cbpb.2024.111060","DOIUrl":"10.1016/j.cbpb.2024.111060","url":null,"abstract":"<div><div>Lysozymes are crucial enzymes involved in the innate immune response against bacterial pathogens. In this study, we identified and characterized a goose-type lysozyme gene (<em>SsLyG</em>) from the black rockfish <em>Sebastes schlegelii</em>, an economically important aquaculture species. The deduced amino acid sequence of <em>SsLyG</em> contains 495 residues, which inculded a signal peptide, an immunoglobulin domain, and a goose egg-white lysozyme (GEWL) domain. Tissue expression analysis revealed the highest <em>SsLyG</em> levels in blood, and its transcription was significantly upregulated in the spleen and kidney upon bacterial and polyI:C challenges. Recombinant SsLyGE (rSsLyGE) exhibited lytic activity against <em>Micrococcus lysodeikticus</em> and concentration-dependent binding ability to <em>Staphylococcus aureus</em> and <em>Micrococcus luteus</em>. Furthermore, rSsLyGE promoted peripheral blood lymphocyte proliferation, enhanced macrophage respiratory burst activity, and increased reactive oxygen species production. RNA interference-mediated knockdown of <em>SsLyG</em> resulted in higher bacterial loads in the liver and spleen after <em>Listonella anguillarum</em> challenge, suggesting its role in early antibacterial defense. Collectively, these findings provide insights into the immune function of <em>SsLyG</em> and its potential application in developing antimicrobial strategies for aquaculture.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"276 ","pages":"Article 111060"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mitochondrial membrane potential and the sources of reactive oxygen species in the hemocytes of the ark clam Anadara kagoshimensis under hypoosmotic stress","authors":"Daria S. Lavrichenko,&nbsp;Elina S. Chelebieva,&nbsp;Ekaterina S. Kladchenko","doi":"10.1016/j.cbpb.2024.111057","DOIUrl":"10.1016/j.cbpb.2024.111057","url":null,"abstract":"<div><div>To compensate for changes in cell volume caused by changes in salt concentration, mollusks use regulatory mechanisms such as the regulation of volume decrease (RVD). This may increase the rate of aerobic metabolism and lead to an increase in reactive oxygen species (ROS). This study examined the production of ROS in the mitochondria of <em>Anadara kagoshiensis</em> hemocytes, the effect of mitochondrial inhibitors on osmotic stability in hemocytes, and the dynamics of changes in ROS levels and mitochondrial membrane potential when RVD is activated under hypo-osmotic conditions. Hemocytes maintained at a control osmolarity of 460 mOsm l⁻¹ showed significant decreases in ROS production following incubation with complex III inhibitors (S3QEL). Hypoosmotic shock stimulated RVD in all experimental groups. The cell volume increased by about 70 % immediately after osmolarity was reduced, and then decreased by about 40 % over the next 30 min. A reduction in osmolarity from about 460 to 200 mOsm l⁻¹ significantly decreased ROS and mitochondrial potentials in <em>A. kashimensis</em> hemocyctes. Inhibitors of mitochondrial complexes did not affect changes in ROS or mitochondria potentials in <em>A kashimiensis</em> hemocytes under hypoosmotic conditions or in hemocyte volume regulation mechanisms.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"276 ","pages":"Article 111057"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of red macroalgae (Galaxaura oblongata) in roach (Rutilus rutilus) fingerling diet: Effects on growth, immunity, oxidative status and intestinal health.","authors":"Seyed Hossein Hoseinifar ,&nbsp;Mohammad Arghideh ,&nbsp;Fatemeh Hosseinpour Delavar ,&nbsp;Marina Paolucci ,&nbsp;Metin Yazici ,&nbsp;Mahsan Bayani ,&nbsp;Hien Van Doan","doi":"10.1016/j.cbpb.2025.111076","DOIUrl":"10.1016/j.cbpb.2025.111076","url":null,"abstract":"<div><div>This study investigates the effects of the red macroalgae <em>Galaxaura oblongata</em> diet supplementation on roach (<em>Rutilus rutilus</em>) fingerlings growth and gene expression related to immunity, the intestinal barrier, and antioxidant status. Roach fingerlings (2.26 ± 0.04 g) were fed a basic diet supplemented with three different percentages of <em>G. oblongata</em> powder: 0.25 % (G1), 0.50 % (G2), and 1.0 % (G3) over 8 weeks, with a control group (C) receiving no supplementation. The study found that growth parameters significantly increased in the G1 and G2 groups compared to the C and G3 groups (<em>P</em> &lt; 0.05). Immune responses, measured by total immunoglobulin (Ig) and lysozyme activity, showed a significant increase in the whole-body extract of the G2 group (P &lt; 0.05) and the skin mucus of all treated groups compared to the control (P &lt; 0.05). <em>G. oblongata</em> supplementation did not significantly affect catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities in the whole-body extract, although GPx activity in skin mucus was significantly higher in the supplemented groups. Additionally, the gene expression of interleukin 1-beta (<em>il-1β)</em>, <em>occludin</em>, <em>gpx</em> and <em>sod</em>, but not <em>Toll-like receptor</em> increased in <em>G. oblongata</em> treated groups. These results suggest that <em>G. oblongata</em> can serve as a beneficial feed additive in the culture of roach fingerlings, enhancing growth and immune function.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111076"},"PeriodicalIF":1.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkalinity exposure induced growth inhibition, intestinal histopathological changes, and down-regulated nutrient transporter expression in Nile Tilapia: The ameliorative role of dietary camel whey protein hydrolysates","authors":"Nawal Alsubaie ,&nbsp;Amany Abdel-Rahman Mohamed ,&nbsp;Mohamed M.M. Metwally ,&nbsp;Tarek Khamis ,&nbsp;Ali Osman ,&nbsp;Badriyah S. Alotaibi ,&nbsp;Areej A. Eskandrani ,&nbsp;Samah S. Abuzahrah ,&nbsp;Yasmina M. Abd-Elhakim ,&nbsp;Abdelhakeem El-Murr ,&nbsp;Rowida E. Ibrahim","doi":"10.1016/j.cbpb.2025.111074","DOIUrl":"10.1016/j.cbpb.2025.111074","url":null,"abstract":"<div><div>Alkaline stress impairs fish productivity and performance and, therefore, is considered one of the major challenges facing aquaculture. In this work, the effects of supplementing diets with camel whey protein hydrolysates (WPH) on growth, digestion, antioxidant capacity, and gene expression were investigated in Nile tilapia (<em>Oreochromis niloticus</em>) under alkaline stress. A total of 160 fish (16.17 ± 0.29 g) were equally assigned into four treatments, with 10 fish in each replicate. The control (C) and WPH groups received the basal diet supplemented with 0 and 75 g/kg WPH, respectively, and were reared in freshwater with an alkalinity of 1.4 mmol NaHCO₃/L. The alkaline-exposed (AK) and AK + WPH groups were subjected to alkaline water (alkalinity = 23.8 mmol NaHCO₃/L) and fed basal and WPH diet, respectively. Alkaline stress depressed the growth performance, digestive enzyme activity, intestinal <em>Lactobacillus</em> count, intestinal morphometrics, growth hormone level, and antioxidant enzyme activity but enhanced leptin hormone level and malondialdehyde (MDA) concentrations in Nile tilapia. Alkaline stress also downregulated the transcription of key intestinal transporter genes. Dietary supplementation with WPH significantly improved growth, digestive enzyme activity, antioxidant capacity, and the gene expression profile of Nile tilapia under alkaline stress. Based on the current results, it was concluded that WPH diet could mitigate negative effects caused by alkaline stress in Nile tilapia, which might support its application as an effective functional protein replacement candidate in aquaculture.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111074"},"PeriodicalIF":1.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fulvic acid on zebrafish (Danio rerio) growth, immunity and antioxidant status","authors":"Seyed Hossein Hoseinifar ,&nbsp;Zohreh Fazelan ,&nbsp;Thora Lieke ,&nbsp;Shiva Nedaei ,&nbsp;Roghieh Safari ,&nbsp;Metin Yazici ,&nbsp;Hien Van Doan","doi":"10.1016/j.cbpb.2025.111073","DOIUrl":"10.1016/j.cbpb.2025.111073","url":null,"abstract":"<div><div>This experiment aimed to determine the efficacy of fulvic acid (FLA) on growth performance, innate immune system, antioxidant parameters, and expression of immune and antioxidant-related genes in zebrafish (<em>Danio rerio</em>). To this end, 12 tanks (3 per group), each containing 50 zebrafish (with an average weight of 85.7 ± 10.05 mg) in 72 L, were assigned to diets containing FLA at four levels: 0 (control), 0.25 (FLA1), 0.5 (FLA2), and 1 (FLA3) g/kg diet. Following an eight-week culture period, no significant differences in growth performance were observed among the treatment groups (<em>P</em> &gt; 0.05). However, lysozyme activity, total immunoglobulin (Ig), and total protein concentrations in whole-body extracts were significantly enhanced in the 0.5–1 g FLA/kg diet groups compared to the other treatments (<em>P</em> &lt; 0.05). No significant differences were observed among the groups in catalase (CAT), glutathione peroxidase (GPx), or superoxide dismutase (SOD) activities (<em>P</em> &gt; 0.05). The supplementation of FLA significantly upregulated the gene expression of interferon-α (IFN-α) and tumor necrosis factor-alpha (TNF-α), with the highest expression observed in the 0.5 g FLA/kg diet group (<em>P</em> &lt; 0.05). Additionally, interleukin 1 (IL-1) expression was markedly elevated in this group in comparison to the other treatments (P &lt; 0.05). While there was a significant increase in GPx gene expression with dietary FLA (P &lt; 0.05), no notable differences were observed among FLA treatments (<em>P</em> &gt; 0.05). CAT gene expression remained consistent across all groups (P &gt; 0.05). In contrast, SOD gene expression significantly increased in response to all FLA-supplemented diets, with the highest level observed in the 0.5 g FLA/kg group (<em>P</em> &lt; 0.05). These findings suggest that FLA may serve as an effective dietary supplement to enhance the immune response and antioxidant capacity in zebrafish.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111073"},"PeriodicalIF":1.9,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanism of Yersinia ruckeri Flagellin C (FliC) induced intestinal inflammation in channel catfish (Ictalurus punctatus)","authors":"Yibin Yang ,&nbsp;Hao He ,&nbsp;Yucong Huang ,&nbsp;Xiaohui Ai ,&nbsp;Xia Zhu ,&nbsp;Fei Yin ,&nbsp;Jingen Xu ,&nbsp;Yuhua Chen","doi":"10.1016/j.cbpb.2025.111072","DOIUrl":"10.1016/j.cbpb.2025.111072","url":null,"abstract":"<div><div><em>Yersinia ruckeri</em> is known to cause enteric red mouth disease (ERM) in channel catfish (<em>Ictalurus punctatus</em>). This study established a model of <em>Y. ruckeri</em>-induced intestinal inflammation in channel catfish. Subsequently, using quantitative polymerase chain reaction (qPCR), gene cloning, recombinant protein expression, protein molecular docking, and tissue pathology techniques, we investigated the role and molecular mechanism of Flagellin C (FliC) from <em>Y. ruckeri</em> in inducing inflammation. The findings indicated that FliC was the main virulence gene in <em>Y. ruckeri</em> responsible for inducing intestinal inflammation. Specifically, FliC bound to the host Toll-like receptor 5 (<em>tlr5</em>), leading to the upregulation of multiple inflammatory factors such as tumor necrosis factor (<em>tnf</em>)<em>-α</em>, interleukin (<em>il</em>)<em>-6</em>, and <em>il-1β</em>, and the activation of the nuclear factor-kappaB (NF-κB) and JAK-STAT signaling pathways, thereby initiating inflammation. The results were validated through experiments conducted both in cellular models and <em>in vivo</em>. In summary, this study identified FliC as a virulence gene in <em>Y. ruckeri</em> infection of channel catfish and elucidated its role in inducing intestinal inflammation.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111072"},"PeriodicalIF":1.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}