Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献

{"title":"(Na+, K+)- ATPase kinetics in Macrobrachium pantanalense: highlighting intra- and interspecific variation within the Macrobrachium amazonicum complex","authors":"Leonardo M. Fabri ,&nbsp;Cintya M. Moraes ,&nbsp;Marina Calixto-Cunha ,&nbsp;Ariadine C. Almeida ,&nbsp;Rogério O. Faleiros ,&nbsp;Daniela P. Garçon ,&nbsp;John C. McNamara ,&nbsp;Samuel C. Faria ,&nbsp;Francisco A. Leone","doi":"10.1016/j.cbpb.2024.110987","DOIUrl":"10.1016/j.cbpb.2024.110987","url":null,"abstract":"<div><p>The <em>Macrobrachium amazonicum</em> complex is composed of at least the <em>Macrobrachium amazonicum</em> and <em>Macrobrachium pantanalense</em> species, with the latter described from specimens originally identified as part of an endemic <em>M. amazonicum</em> population in the Brazilian Pantanal region. While there may be a reproductive barrier between these two <em>Macrobrachium</em> species, both are phylogenetically close, with small genetic distance. However, there is currently no available biochemical information of <em>Macrobrachium pantanalense</em> (Na⁺, K⁺)-ATPase. Here, we report the kinetic characteristics of the gill (Na⁺, K⁺)-ATPase in two populations of <em>M. pantanalense</em> from Baiazinha Lagoon (Miranda, MS, Brazil) and Araguari River (Uberlândia, MG, Brazil), and compare them with <em>Macrobrachium amazonicum</em> populations from the Paraná-Paraguay River Basin. (Na⁺, K⁺)-ATPase activities were 67.9 ± 3.4 and 93.3 ± 4.1 nmol Pi min⁻¹ mg⁻¹ protein for the Baiazinha Lagoon and Araguari River populations, respectively. Two ATP hydrolyzing sites were observed for the Araguari River population while a single ATP site was observed for the Baiazinha Lagoon shrimps. Compared to the Araguari River population, a 3-fold greater apparent affinity for Mg²⁺ and Na⁺ was estimated for the Baiazinha Lagoon population, but no difference in K⁺ affinity and ouabain inhibition was seen. The kinetic differences observed in the gill (Na⁺, K⁺)-ATPase between the two populations of <em>M. pantanalense</em>, compared with those of various <em>M. amazonicum</em> populations, highlight interspecific divergence within the <em>Macrobrachium</em> genus<em>,</em> now examined from a biochemical perspective.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Star1 gene mutation reveals the essentiality of 11-ketotestosterone and glucocorticoids for male fertility in Nile Tilapia (Oreochromis niloticus)","authors":"Yun Su ,&nbsp;You Wu ,&nbsp;Maolin Ye ,&nbsp;Chenhua Zhao ,&nbsp;Lu Li ,&nbsp;Jing Cai ,&nbsp;Tapas Chakraborty ,&nbsp;Lanying Yang ,&nbsp;Deshou Wang ,&nbsp;Linyan Zhou","doi":"10.1016/j.cbpb.2024.110985","DOIUrl":"10.1016/j.cbpb.2024.110985","url":null,"abstract":"<div><p>Steroidogenic acute regulatory protein (Star) plays an essential role in the biosynthesis of corticosteroids and sex steroids by mediating the transport of cholesterol from the outer to the inner membrane of mitochondria. Two duplicated <em>Star</em> genes, namely <em>star1</em> and <em>star2</em>, have been identified in non-mammalian vertebrates. To investigate the roles of <em>star</em> genes in fish steriodogenesis, we generated two mutation lines of <em>star1</em>^{<em>−/−</em>} and <em>star1</em>^{<em>−/−</em>}<em>/star2</em>^{<em>−/−</em>} in Nile tilapia (<em>Oreochromis niloticus</em>). Previous studies revealed that deficiency of <em>star2</em> gene caused delayed spermatogenesis, sperm apoptosis and sterility in male tilapia. Our present data revealed that mutation of <em>star</em> genes impaired male fertility. Disordered seminiferous lobules and spermatic duct obstruction were found in the testis of both types of mutants. Moreover, significant decline in semen volume, sperm abnormality and impaired fertility were also detected in <em>star1</em>^{<em>−/−</em>} and <em>star1</em>^{<em>−/−</em>}<em>/star2</em>^{<em>−/−</em>} males. In <em>star1</em>^{<em>−/−</em>} male fish, lipid accumulation, up-regulation of steroidogenic enzymes, and significant decline of androgens were found. Additionally, hyperplasic interrenal cells, elevated steroidogenic gene expression level and decline of serum glucocorticoids were detected in <em>star1</em> mutants. Intriguingly, either 11-KT or cortisol supplementation successfully rescued the impaired fertility of the <em>star1</em>^{<em>−/−</em>} mutants. Taken together, these results further indicate that Star1 might play critical roles in the production of both 11-KT and glucocorticoids, which are indispensable for the maintenance of male fertility in fish.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of multidomain monothiol glutaredoxin 3 from diploblastic Hydra","authors":"Nusrat Perween ,&nbsp;Komal Pekhale ,&nbsp;Gauri Haval ,&nbsp;Gargi Sirkar ,&nbsp;Ganesh S. Bose ,&nbsp;Smriti P.K. Mittal ,&nbsp;Surendra Ghaskadbi ,&nbsp;Saroj S. Ghaskadbi","doi":"10.1016/j.cbpb.2024.110986","DOIUrl":"10.1016/j.cbpb.2024.110986","url":null,"abstract":"<div><p>Intracellular antioxidant glutaredoxin controls cell proliferation and survival. Based on the active site, structure, and conserved domain motifs, it is classified into two classes. Class I contains dithiol Grxs with two cysteines in the consensus active site sequence CXXC, while class II has monothiol Grxs with one cysteine residue in the active site. Monothiol Grxs can also have an additional N-terminal thioredoxin (Trx)-like domain. Previously, we reported the characterization of Grx1 from <em>Hydra vulgaris</em> (<em>Hv</em>Grx1), which is a dithiol isoform. Here, we report the molecular cloning, expression, analysis, and characterization of another isoform of Grx, which is the multidomain monothiol glutaredoxin-3 from <em>Hydra vulgaris</em> (<em>Hv</em>Grx3). It encodes a protein with 303 amino acids and is significantly larger and more divergent than <em>Hv</em>Grx1. <em>In-silico</em> analysis revealed that Grx1 and Grx3 have 22.5% and 9.9% identical nucleotide and amino acid sequences, respectively. <em>Hv</em>Grx3 has two glutaredoxin domains and a thioredoxin-like domain at its amino terminus, unlike <em>Hv</em>Grx1, which has a single glutaredoxin domain. Like other monothiol glutaredoxins, <em>Hv</em>Grx3 failed to reduce glutathione-hydroxyethyl disulfide. In the whole <em>Hydra</em>, <em>Hv</em>Grx3 was found to be expressed all over the body column, and treatment with H₂O₂ led to a significant upregulation of <em>Hv</em>Grx3. When transfected in HCT116 (human colon cancer cells) cells, <em>Hv</em>Grx3 enhanced cell proliferation and migration, indicating that this isoform could be involved in these cellular functions. These transfected cells also tolerate oxidative stress better.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term dietary teprenone improved thermal tolerance and mitigated liver damage caused by heat stress in juvenile largemouth bass (Micropterus salmoides)","authors":"Hongyun Li ,&nbsp;Feifei Chen ,&nbsp;Mu Qin ,&nbsp;Chenlei Liao ,&nbsp;Yaqi Shi ,&nbsp;Sihan Wu ,&nbsp;Keming Rong ,&nbsp;Xuezhen Zhang","doi":"10.1016/j.cbpb.2024.110984","DOIUrl":"10.1016/j.cbpb.2024.110984","url":null,"abstract":"<div><p>Heat stress seriously threatens fish survival and health, demanding immediate attention. Teprenone is a gastric mucosal protective agent that can induce heat shock protein expression. This research investigated the effects of teprenone on largemouth bass (<em>Micropterus salmoides</em>) subjected to heat stress. Juvenile fish were assigned to different groups: group C (control group, 0 mg teprenone/kg diet), T₀, T₂₀₀, T₄₀₀, and T₈₀₀ (0, 200, 400, and 800 mg teprenone/kg diet, respectively), which were fed for 3 days, followed by a day without the diet. All groups except group C were subjected to acute heat stress (from 24 °C to 35 °C at 1 °C per hour and then maintained at 35 °C for 3 h). The results were as follows: The critical thermal maxima were significantly higher in the T₂₀₀, T₄₀₀, and T₈₀₀ groups compared with the T₀ group (<em>P</em> &lt; 0.05). Heat stress caused severe damage to the tissue morphology of the liver, while teprenone significantly reduced this injury (<em>P</em> &lt; 0.05). Serum cortisol concentration decreased gradually as teprenone concentration increased, and the lowest concentration was observed in the T₈₀₀ group (<em>P</em> &lt; 0.05). Compared with the T₀ group, the serum activities of aspartate aminotransferase, alanine aminotransferase, and gamma-glutamyl transferase were significantly lower in the T₂₀₀, T₄₀₀, and T₈₀₀ groups (<em>P</em> &lt; 0.05). The liver activities of catalase, total superoxide dismutase, and peroxidase were significantly higher in the T₂₀₀ group than in the T₀ group (<em>P &lt;</em> 0.05). Transcript levels of the heat shock proteins (<em>hsp90</em>, <em>hsp70</em>, <em>hspa5</em>, and <em>hsf1</em>) and caspase family (<em>caspase3</em> and <em>caspase9</em>) in the liver of the T₂₀₀ group were significantly higher than those of the T₀ group (<em>P</em> &lt; 0.05). Western blot results showed that HSP70 and HSPA5 in the liver were significantly upregulated in the T₂₀₀ group compared with the T₀ group (<em>P</em> &lt; 0.05). In summary, dietary teprenone improved thermal tolerance, alleviated heat stress damage in the liver, enhanced antioxidant capacity, and upregulated heat shock proteins in juvenile largemouth bass. This study offers theoretical support for applying teprenone in aquaculture to reduce financial losses caused by abiotic factors.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous glands of the striped toad, Rhinella crucifer (Wied-Neuwied, 1821) (Amphibia: Bufonidae): Histological study and bioactivities of glandular secretions","authors":"Géssica Gomes Barbosa ,&nbsp;Tulíbia Laurindo Silva ,&nbsp;Ana Patrícia Silva de Oliveira ,&nbsp;Thâmarah de Albuquerque Lima ,&nbsp;Pollyanna Michelle da Silva ,&nbsp;Carlos José Correia de Santana ,&nbsp;Jeymesson Raphael Cardoso Vieira ,&nbsp;Dyeime Ribeiro de Sousa ,&nbsp;Francisco Assis Leite Souza ,&nbsp;Rafael Pereira ,&nbsp;Russolina Benedeta Zingali ,&nbsp;Romero Marcos Pedrosa Brandão Costa ,&nbsp;Patrícia Maria Guedes Paiva ,&nbsp;Gilberto Gonçalves Rodrigues ,&nbsp;Mariana S. Castro ,&nbsp;Thiago Henrique Napoleão","doi":"10.1016/j.cbpb.2024.110983","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110983","url":null,"abstract":"<div><p>This study investigated the morphology of <em>Rhinella crucifer</em> cutaneous glands, as well as the protein/peptide profiles and bioactivities of body gland secretions (BGS) and parotoid macrogland secretions (PS). The parotoid as well as dorsal and ventral skin fragments of male and female individuals were processed for histological analysis. The protein and peptide profiles of male and female gland secretions were evaluated. Male secretions were also assessed for proteolytic, trypsin inhibiting, hemagglutinating, hemolytic, antimicrobial, and anticoagulant activities. The <em>R. crucifer</em> skin structure presented protuberances that are clearly visible and formed by the integument, which has cutaneous glands throughout the body. An average of 438 and 333 glands were identified in males in females, respectively. No significant differences were observed in the distribution of glands across the body as well as for area and perimeter of glands. Differences were observed in protein composition between the PS and BGS from males and females, and secretions from animals collected from undisturbed and anthropogenically disturbed areas. Proteins with similarities to catalase and elongation factor 1-alpha were detected in the PS. Zymography revealed proteolytic activity in both male BGS and PS. Male BGS showed antibacterial activity against <em>Enterococcus faecalis</em> and <em>Escherichia coli</em> and anticoagulant activity, being able to prolong prothrombin time by 6.34-fold and activated partial thromboplastin time by 2.17-fold. Finally, male PS and BGS caused a maximum hemolysis degree of 1.4%. The data showed that the cutaneous secretions of <em>R. crucifer</em> are potentially promising for biotechnological prospecting.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140824850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sub-chronic polystyrene nanoplastics exposure on hematology, histology, and endoplasmic reticulum stress-related protein expression in Nile tilapia (Oreochromis niloticus)","authors":"Vadavanath Prabhakaran Vineetha ,&nbsp;Kummari Suresh ,&nbsp;Devika Pillai","doi":"10.1016/j.cbpb.2024.110982","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110982","url":null,"abstract":"<div><p>Nanoplastics (NPs) are one of the most hazardous marine litters, having the potential to cause far-reaching impacts on the environment and humankind. The effect of NPs on fish health has been studied, but their impact on the subcellular organelles remains unexplored. The present investigation studied the possible implications of polystyrene-nanoplastics (PS-NPs) on the hematology, tissue organization, and endoplasmic reticulum (ER) stress-related proteins in Nile tilapia <em>(Oreochromis niloticus).</em> Fish were exposed to ∼100 nm PS-NPs at environmentally relevant (0.1 mg/L), and sublethal (1, 10 mg/L) concentrations for 14 days through water exposure. The growth performance and hematological parameters such as erythrocytes, hemoglobin, hematocrit, and leucocytes decreased, while thrombocytes increased with PS-NPs dose-dependently. The gills, liver, kidney, and heart tissues displayed increasing degrees of pathology with increased concentrations of PS-NPs. The gills showed severe epithelial hyperplasia and lamellar fusion. The liver had an abstruse cellular framework, membrane breakage, and vacuolation. While glomerular and tubular atrophy was the most prominent pathology in the kidney tissue, the heart displayed extensive myofibrillar loss and disorderly arranged cardiac cells. The ER-stress-related genes such as <em>bip, atf6, ire1, xbp1, pkr,</em> and apoptotic genes such as <em>casp3a,</em> and <em>bax</em> were over-expressed, while, the anti-apoptotic <em>bcl2</em> was under-expressed with increasing concentrations of PS-NPs. Immunohistochemistry and blotting results of GRP78, CHOP, EIF2S, and ATF6 in gills, liver, kidney, and heart tissues affirmed the translation to ER stress proteins. The results revealed the sub-lethal adverse effects and the activation of the ER-stress pathway in fish with sub-chronic exposure to PS-NPs.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140823291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphisms in the cysteine dioxygenase gene and their association with taurine content in the Pacific oyster Crassostrea gigas","authors":"Xin Qiao ,&nbsp;Ning Kong ,&nbsp;Shiqing Sun ,&nbsp;Xiang Li ,&nbsp;Chunyu Jiang ,&nbsp;Cong Luo ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.cbpb.2024.110981","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110981","url":null,"abstract":"<div><p>The Pacific oyster <em>Crassostrea gigas</em> is rich in taurine, which is crucial for its adaptation to the fluctuating intertidal environment and presents significant potential in improving taurine nutrition and boosting immunity in humans. Cysteine dioxygenase (CDO) is a key enzyme involved in the initial step of taurine biosynthesis and plays a crucial role in regulating taurine content in the body. In the present study, polymorphisms of CDO gene in <em>C. gigas</em> (<em>Cg</em>CDO) and their association with taurine content were evaluated in 198 individuals. A total of 24 single nucleotide polymorphism (SNP) loci were identified in the exonic region of <em>Cg</em>CDO gene by direct sequencing. Among these SNPs, c.279G&gt;A and c.287C&gt;A were found to be significantly associated with taurine content, with the GG and AA genotype at the two loci exhibiting enhanced taurine accumulation (<em>p</em> &lt; 0.05). Haplotype analysis revealed that the 279GG/287AA haplotype had the highest taurine content of 29.24 mg/g, while the 279AA/287CC haplotype showed the lowest taurine content of 21.19 mg/g. These results indicated that the SNPs of <em>Cg</em>CDO gene could influence the taurine content in <em>C. gigas</em> and have potential applications in the selective breeding of high-taurine varieties.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140638723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: The boring sponge (Pione vastifica, Hancock, 1849) induces oxidative stress in the Pacific oyster (Magallana gigas, Thunberg, 1793)","authors":"Elina S. Chelebieva,&nbsp;Daria S. Lavrichenko,&nbsp;Olga L. Gostyukhina,&nbsp;Maria S. Podolskaya,&nbsp;Ekaterina S. Kladchenko","doi":"10.1016/j.cbpb.2024.110980","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110980","url":null,"abstract":"<div><p>Boring sponge infection affects growth, development and reduces the soft tissue weight of oysters. In this study, we investigated the effects of boring sponge on the activity of three antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GP)) in the mantle, and the production of reactive oxygen species (ROS) and potential genotoxicity in hemocytes of the Pacific oyster <em>Magallana gigas</em>. Our results showed a significant increase in ROS production and DNA damage in hemocytes. Notably, the activity of SOD, CAT, and GP in the mantle was not significantly affected by boring sponge infection. Collectively, these results suggest that sponge invasion may cause oxidative stress in Pacific oyster hemocytes through ROS overproduction.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140631431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus × female Channa maculata) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)","authors":"Huiling Deng ,&nbsp;Shucheng Zheng ,&nbsp;Yingying Li ,&nbsp;Xubing Mo ,&nbsp;Jian Zhao ,&nbsp;Jiyuan Yin ,&nbsp;Cunbin Shi ,&nbsp;Qing Wang ,&nbsp;Yingying Wang","doi":"10.1016/j.cbpb.2024.110971","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110971","url":null,"abstract":"<div><p>Hybrid snakehead (male <em>Channa argus</em> × female <em>Channa maculata</em>) is an emerging fish breed with increasing production levels. However, infection with hybrid snakehead rhabdovirus (HSHRV) critically affects hybrid snakehead farming. In this study, a fish cell line called CAMK, derived from the kidneys of hybrid snakehead, was established and characterized. CAMK cells exhibited the maximum growth rate at 28 °C in Leibovitz's-15 medium supplemented with 10% fetal bovine serum(FBS). Karyotyping revealed diploid chromosomes in 54% of the cells at the 50th passage (2n = 66), and 16S rRNA sequencing validated that CAMK cells originated fromhybrid snakehead, and the detection of kidney-specific antibodies suggested that it originated from kidney. <em>.</em>The culture was free from mycoplasma contamination, and the green fluorescent protein gene was effectively transfected into CAMK cells, indicating their potential use for in vitro gene expression investigations. Furthermore, qRT-PCR and immunofluorescence analysis revealed that HSHRV could replicate in CAMK cells, indicating that the cells were susceptible to the virus. Transmission electron microscopy revealed that the viral particles had bullet-like morphology. The replication efficiency of HSHRV was 10^7.33 TCID₅₀/mL. Altogether, we successfully established and characterized a kidney cell line susceptible to the virus. These findings provide a valuable reference for further genetic and virological studies.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140645247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of transcription factor CREB3L2 and CREB3L3 and their role in melanogenesis in Pacific oysters (Crassostrea gigas)","authors":"Kunyin Jiang ,&nbsp;Hong Yu ,&nbsp;Lingfeng Kong ,&nbsp;Shikai Liu ,&nbsp;Qi Li","doi":"10.1016/j.cbpb.2024.110970","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110970","url":null,"abstract":"<div><p>Colorful shells in mollusks are commonly attributable to the presence of biological pigments. In Pacific oysters, the inheritance patterns of several shell colors have been investigated, but little is known about the molecular mechanisms of melanogenesis and pigmentation. cAMP-response element binding proteins (CREB) are important transcription factors in the cAMP-mediated melanogenesis pathway. In this study, we characterized two <em>CREB</em> genes (<em>CREB3L2</em> and <em>CREB3L3</em>) from Pacific oysters. Both of them contained a conserved DNA-binding and dimerization domain (a basic-leucine zipper domain). <em>CREB3L2</em> and <em>CREB3L3</em> were expressed highly in the mantle tissues and exhibited higher expression levels in the black-shell oyster than in the white. Masson-Fontana melanin staining and immunofluorescence analysis showed that the location of CREB3L2 protein was generally consistent with the distribution of melanin in oyster edge mantle. Dual-luciferase reporter assays revealed that CREB3L2 and CREB3L3 could activate the <em>microphthalmia-associated transcription factor</em> (<em>MITF</em>) promoter and this process was regulated by the level of cAMP. Additionally, we found that cAMP regulated melanogenic gene expression through the CREB-MITF-TYR axis. These results implied that <em>CREB3L2</em> and <em>CREB3L3</em> play important roles in melanin synthesis and pigmentation in Pacific oysters.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140545769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}