Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献

{"title":"Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal","authors":"Osman Sabri Kesbiç ,&nbsp;Ümit Acar ,&nbsp;Fevziye Işıl Kesbiç ,&nbsp;Sevdan Yılmaz","doi":"10.1016/j.cbpb.2024.110968","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110968","url":null,"abstract":"<div><p>The need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, <em>Oncorhynchus mykiss</em>. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, and immune (TNF-α, IL1-ß and Il-8) and growth-related (GH and IGF<img>I) gene expression were investigated. Five experimental diets (45% protein and 20% lipid) differed in replacement level of FM by PP at rates of 0% (control (PP0)), 25% (PP25), 50%(PP50), 75%(PP75) and 100%(PP100). Fish were fed with experimental diets in triplicate twice daily. The best growth performance was obtained in PP0 and PP25 groups. While fat ratios of fish fillets significantly differed (<em>p</em> &lt; 0.05), there was no significant effects on protein ratios (<em>p</em> &lt; 0.05). There was no significant change in the hematological values of fish, except those fed the PP100 diets, which displayed a reduction in eyrthocyte counts, hemoglobin content and hematocrit. As PP supplementation increased fish showed elevated serum glucose, total protein, cholesterol and myeloperoxidase activity and decreased glutamic pyruvic transaminase and alkaline phosphatase activity. Fish fed diets with between 25 and 75% replacement showed a decline in lactic acid bacteria in the gut. Significant increases in expression were observed in the liver of the PP25 fish relative to the 0% control for all immune and growth-related genes except for IL1-ß. These data suggest that up to 25% of FM can be replaced by PP without any adverse effects on rainbow trout.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140548638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum","authors":"Marcela C. Sena ,&nbsp;Fábio C. Sterzelecki ,&nbsp;Paola Fabiana F. Gomes ,&nbsp;Helen Cristiane A. Souza ,&nbsp;Nuno Filipe A. Correia de Melo ,&nbsp;J. Natividade ,&nbsp;B. Baldisserotto ,&nbsp;Glauber David A. Palheta ,&nbsp;Simone do Socorro D. Santos","doi":"10.1016/j.cbpb.2024.110969","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110969","url":null,"abstract":"<div><p>Blood analysis is an important tool for monitoring the health status of fish, but the time between collection and analysis can affect the outcome of the analysis. This study sought to determine the maximum time refrigerated blood and frozen plasma samples of the tambaqui, <em>Colossoma macropomum</em>, can be stored without affecting analysis. Samples from 12 fish were obtained, stored under refrigeration at 4 °C and evaluated after 0, 24, 48, 72, and 96 h, while samples from 14 fish were centrifuged, and the resulting plasma was frozen at −20 °C and then evaluated after 0, 8, 12, 16 and 20 weeks. The parameters analyzed were hematocrit (Ht), hemoglobin content (Hb), total erythrocytes (RBC), total (WBC) and differential leukocytes, total thrombocytes (TC), glucose content (Glc), total protein (TP), triglyceride content (TG), total cholesterol (CoT), and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). For refrigerated whole blood samples, mean corpuscular hemoglobin content (MCHC) showed a transient decline in 24 h, and there were decreases in WBC, TC, Glc and TG that persisted until the 72 h sample point (for Glc and TG) or persisted until the 96 h sample point (for WBC and TC). A decrease in RBC was noted from 48 h on, while ALT was significantly higher in the 96 h sample. Significant decreases in lymphocytes, monocytes, neutrophils and eosinophils were noted from 48 h of storage on, while a significant decline in basophil counts were noted over the last two sampled timepoints. The coefficient of variation was greatest at the 96 h timepoint, indicating increased variability in measured parameters after 4 d of refrigeration. Plasma samples frozen at −20 °C showed a significant variation in ALT after 8 weeks, and increases in TP and TG after 20 weeks. Therefore, it is recommended that refrigerated tambaqui whole blood samples be analyzed within 24 h and frozen tambaqui plasma samples analyzed within 8 weeks.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140546046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA miR-7-5p targets MARK2 to control metamorphosis in Galeruca daurica","authors":"Hai-Chao Wang ,&nbsp;Ling Li ,&nbsp;Jing-Hang Zhang ,&nbsp;Zhi-Han Yao ,&nbsp;Bao-Ping Pang","doi":"10.1016/j.cbpb.2024.110967","DOIUrl":"10.1016/j.cbpb.2024.110967","url":null,"abstract":"<div><p>The <em>MARK2</em> gene, coding microtubule affinity-regulating kinase or serine/threonine protein kinase, is an important modulator in organism microtubule generation and cell polarity. However, its role in the metamorphosis of insects remains unknown. In this study, we found a conserved miRNA, <em>miR-7-5p</em>, which targets <em>MARK2</em> to participate in the regulation of the larval-pupal metamorphosis in <em>Galeruca daurica</em>. The dual luciferase reporter assay showed that <em>miR-7-5p</em> interacted with the 3’ UTR of <em>MARK2</em> and repressed its expression. The expression profiling of <em>miR-7-5p</em> and <em>MARK2</em> displayed an opposite trend during the larval-adult development process. In <em>in-vivo</em> experiments, overexpression of <em>miR-7-5p</em> by injecting <em>miR-7-5p</em> agomir in the final instar larvae down-regulated <em>MARK2</em> and up-regulated main ecdysone signaling pathway genes including <em>E74</em>, <em>E75</em>, <em>ECR</em>, <em>FTZ-F1</em> and <em>HR3</em>, which was similar to the results from knockdown of <em>MARK2</em> by RNAi. In contrast, repression of <em>miR-7-5p</em> by injecting <em>miR-7-5p</em> antagomir obtained opposite effects. Notably, both overexpression and repression of <em>miR-7-5p</em> in the final instar larvae caused abnormal molting and high mortality during the larval-pupal transition, and high mortality during the pupal-adult transition. The 20-hydroxyecdysone (20E) injection experiment showed that 20E up-regulated <em>miR-7-5p</em> whereas down-regulated <em>MARK2</em>. This study reveals that the accurate regulation of miRNAs and their target genes is indispensable for insect metamorphosis.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140195073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of adenosine monophosphate deaminase 1 and the correlation analysis between its mRNA expression levels and inosine monophosphate content in large yellow croaker (Larimichthys crocea)","authors":"Liangyin He ,&nbsp;Xiaoli Shi ,&nbsp;Kunhuang Han ,&nbsp;Weiqing Huang ,&nbsp;Delong Chen ,&nbsp;Zhidi Lian ,&nbsp;Shaojiang Ruan","doi":"10.1016/j.cbpb.2024.110966","DOIUrl":"10.1016/j.cbpb.2024.110966","url":null,"abstract":"<div><p>Declining flesh quality has drawn considerable attention in the farmed large yellow croaker (LYC; <em>Larimichthys crocea</em>) industry. Inosine monophosphate (IMP) is the primary flavor substance in aquatic animals. Adenosine monophosphate deaminase 1 (AMPD1) plays a critical role in IMP formation by catalyzing the deamination of AMP to IMP in the purine nucleotide cycle. To further evaluate the correlation between <em>ampd1</em> mRNA expression levels and IMP content in the LYC muscle tissue, the relevant open reading frame (ORF) of <em>L. crocea</em> (<em>Lcampd1</em>) was cloned, and the IMP content and <em>Lcampd1</em> mRNA expression in the muscles of LYCs of different sizes were examined. The ORF cDNA of <em>Lcampd1</em> was 2211 bp in length and encoded a polypeptide of 736 amino acids (AAs). The deduced protein, LcAMPD1, possesses conserved AMPD active regions (SLSTDDP) and shows high homology with <span><math><mtext>AMPD proteins</mtext></math></span> of other teleost fishes. The genomic DNA sequence of <em>Lcampd1</em> exhibits a high degree of evolutionary conservation in terms of structural organization among species. Phylogenetic analysis of the deduced AA sequence revealed that teleost fish and mammalian AMPD1 were separate from each other and formed a cluster with AMPD3, suggesting that AMPD1 and AMPD3 arose by duplication of a common primordial gene. In healthy LYC, <em>Lcampd1</em> mRNA was expressed only in the muscle tissue. The IMP content in the muscle of LYCs with different average body weights was measured by high-performance liquid chromatography; the results showed that the IMP content in the muscle of LYCs with greater body weight was significantly higher than that in LYC with lower body weight. Moreover, a similar trend in <em>Lcampd1</em> expression was observed in these muscle tissues. The Pearson correlation analysis further showed that the <em>Lcampd1</em> mRNA expression was positively correlated with IMP content in the muscles of different-sized LYCs. These results suggest the potential function of <em>Lcampd1</em> in determining the IMP content in LYC and provide a theoretical basis for flesh quality improvement, as well as a scientific basis for the development of the molecular breeding of LYC.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary type (carnivore, herbivore and omnivore) and animal species modulate the nutritional metabolome of terrestrial species.","authors":"Lola Llobat ,&nbsp;Pilar Soriano ,&nbsp;Francesco Bordignon ,&nbsp;Trinidad de Evan ,&nbsp;Torben Larsen ,&nbsp;Pablo Jesús Marín-García","doi":"10.1016/j.cbpb.2024.110965","DOIUrl":"10.1016/j.cbpb.2024.110965","url":null,"abstract":"<div><p>Ecometabolomics could be implemented as a powerful tool in molecular ecology studies, but it is necessary to know the baseline of certain metabolites and understand how different traits could affect the metabolome of the animals. Therefore, the main objective of this study was to provide values for the nutritional metabolome profile of different diet groups and animal species, as well as to study the differences in the metabolomic profile due to the effect of diet type and species. To achieve this goal, blood samples were taken from healthy animals (<em>n</em> = 43) of different species: lion (<em>Panthera leo)</em>, jaguar (<em>Panthera onca)</em>, chimpanzee (<em>Pan troglodytes)</em>, bison (<em>Bison bison)</em>, gazelle (<em>Gazella cuvieri)</em> and fallow deer (<em>Dama dama</em>), and with different types of diet (carnivore, herbivore and omnivore). Each blood sample was analysed to determine nutritional metabolites. The main results this study provides are the nutritional metabolic profile of these animals based on the type of diet and the animal species. A significant effect of the dietary type was found on nutritional metabolite levels, with those metabolites related to protein metabolism (total protein and creatine) being higher in carnivores. There is also an effect of the species on nutritional metabolites, observing a metabolome differentiation between lion and jaguar. In the case of herbivores, bison showed higher levels of uric acid and cholesterol, and lower urea levels than gazelle and fallow deer<em>.</em> More molecular ecology studies are needed to further the knowledge of the metabolism of these animals.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1096495924000320/pdfft?md5=e9dbaf5bbaf85db062dd5cf64e3a551c&pid=1-s2.0-S1096495924000320-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet with different concentrations of lychee peel flour modulates oxidative stress parameters and antioxidant activity in zebrafish","authors":"João Paulo Lima de Oliveira ,&nbsp;William Franco Carneiro ,&nbsp;Kiara Cândido Duarte da Silva ,&nbsp;Moises Silvestre de Azevedo Martins ,&nbsp;Stefania Priscilla de Souza ,&nbsp;Bárbara do Carmo Rodrigues Virote ,&nbsp;Isaac Filipe Moreira Konig ,&nbsp;Eduardo Valério de Barros Vilas Boas ,&nbsp;Luis David Solis Murgas ,&nbsp;Elisângela Elena Nunes Carvalho","doi":"10.1016/j.cbpb.2024.110964","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110964","url":null,"abstract":"<div><p>The agri-food industry generates substantial waste, leading to significant environmental impacts. Lychee (<em>Litchi chinensis</em> Sonnerat), which is rich in bioactive compounds in its peel, pulp, and seeds, offers an opportunity for waste use. This study aimed to evaluate the effects of supplementing a high-carbohydrate diet with varying levels of lychee peel flour on lipid metabolism biomarkers and oxidative stress in a zebrafish (<em>Danio rerio</em>) model. A total of 225 zebrafish, approximately four months old, were divided into five groups: control, high-carbohydrate (HC), HC2%, HC4%, and HC6%. The study did not find significant differences in the growth performance of zebrafish in any group. However, the HC6% group exhibited a significant decrease in glucose and triglyceride levels compared with the HC group. Furthermore, this group showed enhanced activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), along with reduced levels of malondialdehyde (MDA). Increased antioxidant activity was also evidenced by DPPH⁻, ABTS⁺, and β-carotene/Linoleic acid assays in the HC6% group. A positive correlation was identified between SOD/CAT activity and in vitro antioxidant assays. These findings suggest that dietary supplementation with 6% lychee peel flour can significantly modulate glucose homeostasis, lipid metabolism, and antioxidant activity in zebrafish.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140016312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary: Analyzing invertebrate bitopic cadherin G protein-coupled receptors that bind Cry toxins of Bacillus thuringiensis","authors":"Li Liu,&nbsp;Lee A. Bulla Jr","doi":"10.1016/j.cbpb.2024.110963","DOIUrl":"https://doi.org/10.1016/j.cbpb.2024.110963","url":null,"abstract":"","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1096495924000307/pdfft?md5=258c999b6ca27762ac1b6c36079e7b50&pid=1-s2.0-S1096495924000307-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140016311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal dietary lipid levels alleviated adverse effects of high temperature on growth, lipid metabolism, antioxidant and immune responses in juvenile turbot (Scophthalmus maximus L.)","authors":"Wencong Zhang ,&nbsp;Zhijie Dan ,&nbsp;Jichang Zheng ,&nbsp;Jianlong Du ,&nbsp;Yongtao Liu ,&nbsp;Zengqi Zhao ,&nbsp;Ye Gong ,&nbsp;Kangsen Mai ,&nbsp;Qinghui Ai","doi":"10.1016/j.cbpb.2024.110962","DOIUrl":"10.1016/j.cbpb.2024.110962","url":null,"abstract":"<div><p>Fish physiological health is often negatively impacted by high-temperature environments and there are few studies on how dietary lipids affect fish growth and physiology when exposed to heat stress. The main objective of this research was to examine the impact of dietary lipid levels on growth and physiological status of juvenile turbot (<em>Scophthalmus maximus</em> L.) and determine if dietary lipid concentration could alleviate the possible adverse effects of heat stress. Five diets containing 6.81%, 9.35%, 12.03%, 14.74%, and 17.08% lipid, respectively, were formulated and fed to turbot (initial weight 5.13 ± 0.02 g) under high-temperature conditions (24.0–25.0 °C). Meanwhile, the diet with 12.03% lipid (considered by prior work to be an optimal dietary lipid level) was fed to turbot of the same size at normal temperature. Results suggested that, among the different dietary lipid levels under high-temperature conditions, fish fed the optimal lipid (12.03%) exhibited better growth compared to non-optimal lipid groups, as evidenced by higher weight gain and specific growth rate. Simultaneously, the optimal lipid diet may better maintain lipid homeostasis, as attested by lower liver and serum lipid, along with higher liver mRNA levels of lipolysis-related genes (<em>pgc1α</em>, <em>lipin1</em>, <em>pparα</em>, <em>lpl</em> and <em>hl</em>) and lower levels of synthesis-related genes (<em>lxr</em>, <em>fas</em>, <em>scd1</em>, <em>pparγ</em>, <em>dgat1</em> and <em>dgat2</em>). Also, the optimal lipid diet might mitigate oxidative damage by improving antioxidant enzyme activity, decreasing malondialdehyde levels, and up-regulating oxidation-related genes (<em>sod1</em>, <em>sod2</em>, <em>cat</em>, <em>gpx</em> and <em>ho-1</em>). Furthermore, the optimal lipid may enhance fish immunity, as suggested by the decrease in serum glutamic-oxalacetic/pyruvic transaminase activities, down-regulation of pro-inflammatory genes and up-regulation of anti-inflammation genes. Correspondingly, the optimal lipid level suppressed MAPK signaling pathway via decreased phosphorylation levels of p38, JNK and ERK proteins in liver. In summary, the optimal dietary lipid level facilitated better growth and physiological status in turbot under thermal stress.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary l-glutamine supplementation on the intestinal physiology and growth during Solea senegalensis larval development","authors":"Ana Catarina Matias ,&nbsp;Ana Rita Viegas ,&nbsp;Ana Couto ,&nbsp;Cátia Lourenço-Marques ,&nbsp;Cláudia Aragão ,&nbsp;Sara Castanho ,&nbsp;Margarida Gamboa ,&nbsp;Ana Candeias-Mendes ,&nbsp;Florbela Soares ,&nbsp;Teresa Modesto ,&nbsp;Pedro Pousão-Ferreira ,&nbsp;Laura Ribeiro","doi":"10.1016/j.cbpb.2024.110961","DOIUrl":"10.1016/j.cbpb.2024.110961","url":null,"abstract":"<div><p>The maturation of the intestinal digestive and absorptive functions might limit the amount of absorbed nutrients to fulfil the high requirements of the fast-growing marine fish larva. Glutamine (Gln) has been described to improve intestinal epithelium functions, due to its involvement in energy metabolism and protein synthesis. The purpose of this study was to evaluate dietary 0.2% Gln supplementation on aspects of intestinal physiology, protein metabolism and growth-related genes expression in Senegalese sole larvae. Experiment was carried out between 12 and 33 days post hatching (DPH) and fish were divided into two experimental groups, one fed <em>Artemia</em> spp. (CTRL) and the other fed <em>Artemia</em> spp. supplemented with Gln (GLN). GLN diet had two times more Gln than the CTRL diet. Samples were collected at 15, 19, 26 and 33 DPH for biometry, histology, and digestive enzymes activity, and at 33 DPH for gene expression, protein metabolism and AA content determination. Growth was significantly higher for Senegalese sole fed GLN diet, supported by differences on protein metabolism and growth-related gene expression. Slight differences were observed between treatments regarding the intestinal physiology. Overall, GLN diet seems to be directed to enhance protein metabolism leading to higher larval growth.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139926965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dramatic genome-wide reprogramming of mRNA in hypometabolic muscle","authors":"Nicholas J. Hudson ,&nbsp;Rebecca L. Cramp ,&nbsp;Craig E. Franklin","doi":"10.1016/j.cbpb.2024.110952","DOIUrl":"10.1016/j.cbpb.2024.110952","url":null,"abstract":"<div><p>In response to seasonal droughts, the green striped burrowing frog <em>Cyclorana alboguttata</em> enters a reversible hypometabolic state called aestivation where heart rate and oxygen consumption can be reduced despite warm (&gt;25C°) ambient temperatures. With a view to understanding molecular mechanisms we profiled aestivating versus control gastrocnemius muscle using mRNA sequencing. This indicated an extensive metabolic reprogramming, with nearly a quarter of the entire transcriptome (3996 of 16,960 mRNA) exhibiting a nominal &gt;2-fold change. Consistent with a physiological adaptation to spare carbohydrate reserves, carbohydrate catabolism was systemically downregulated. A 630-fold downregulation of <em>ENO3</em> encoding the enolase enzyme was most striking. The 590 frog orthologs of mRNA encoding the mitoproteome were, viewed as a population, significantly downregulated during aestivation, although not to the same extent as mRNA encoding carbohydrate catabolism. Prominent examples include members of the TCA cycle (<em>IDH2</em>), electron transport chain (<em>NDUFA6</em>), the ATP synthase complex (<em>ATP5F1B</em>) and ADP/ATP intracellular transport (<em>SLC25A4</em>). Moreover, mRNA derived from the mt genome itself (e.g. <em>mt-ND1</em>) were also downregulated. Most prominent among the upregulated mRNA are those encoding aspects of regulated proteolysis including the proteosome (e.g. <em>PSME4L</em>), peptidases (<em>USP25</em>), atrogins (<em>FBXO32</em>) and ubiquitination (<em>VCP</em>). Finally, we note the ∼5-fold upregulation of the mRNA <em>EIFG3</em> that encodes part of the EIF4F complex. This possesses global control of protein synthesis. Given protein synthesis is repressed in aestivating frogs this indicates the skeletal musculature is poised for accelerated translation of mRNA upon emergence, supporting a strategy to rapidly restore function when the summer rains come.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139736830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}