Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献_第10页

Identification and organ-specific patterns of expression of two metallothioneins in the sentinel species Gammarus fossarum 两种金属硫蛋白在哨点物种斑马鱼中的鉴定和器官特异性表达模式。

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-10-10 DOI: 10.1016/j.cbpb.2023.110907

Davide Degli Esposti , Auréline Lalouette , Karen Gaget , Louveline Lepeule , Zineb Chaabi , Maxime Leprêtre , Anabelle Espeyte , Nicolas Delorme , Hervé Quéau , Laura Garnero , Federica Calevro , Arnaud Chaumot , Olivier Geffard

{"title":"Identification and organ-specific patterns of expression of two metallothioneins in the sentinel species Gammarus fossarum","authors":"Davide Degli Esposti , Auréline Lalouette , Karen Gaget , Louveline Lepeule , Zineb Chaabi , Maxime Leprêtre , Anabelle Espeyte , Nicolas Delorme , Hervé Quéau , Laura Garnero , Federica Calevro , Arnaud Chaumot , Olivier Geffard","doi":"10.1016/j.cbpb.2023.110907","DOIUrl":"10.1016/j.cbpb.2023.110907","url":null,"abstract":"<div>Metal pollution is a major concern for aquatic environments. Widespread contamination by various trace metal ions has been described in freshwater streams as well as their subsequent bioaccumulation, potentially leading to toxicity and trophic transfer. Metallothioneins constitute an evolutionary conserved family of low molecular weight, cysteine-rich, metal-chelating proteins, whose known physiological functions are the maintenance of the homeostasis of essential metals, the detoxification of non-essential metals, and the protection against oxidative stress and free radicals.In this study, we identified two metallothionein-coding transcripts, mt1 and mt2, in the transcriptome of the amphipod Gammarus fossarum, a sentinel species widely used to assess the quality of watersheds. For the first time, we investigated the organ-specific patterns of expression of these two mt transcripts at the individual level in the gills and the caeca of this small crustacean. In silico analysis and experimental exposures to environmentally relevant concentrations of cadmium, zinc and silver showed that G. fossarum mt1 induction is stronger after Cd exposure compared to the other tested metals. G. fossarum mt1 was more significantly induced in the caeca than in the gills of exposed organisms for any metal exposure, while G. fossarum mt2 was, at least at the individual level, more inducible in the gills than in the caeca of G. fossarum exposed to Cd and Zn.Our results provide new genetic resources that will help to improve the understanding of metal homeostasis in this sentinel species.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41220759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of digestive enzyme profiles in newly-hatched (Zoea I) larvae of the mud crab Scylla serrata to intermittent food availability and food deprivation 锯缘青蟹新孵化幼虫的消化酶谱对间歇性食物供应和食物剥夺的反应。

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-10-08 DOI: 10.1016/j.cbpb.2023.110906

Jerome Genodepa , Chaoshu Zeng , Thane A. Militz , Paul C. Southgate

{"title":"Responses of digestive enzyme profiles in newly-hatched (Zoea I) larvae of the mud crab Scylla serrata to intermittent food availability and food deprivation","authors":"Jerome Genodepa , Chaoshu Zeng , Thane A. Militz , Paul C. Southgate","doi":"10.1016/j.cbpb.2023.110906","DOIUrl":"10.1016/j.cbpb.2023.110906","url":null,"abstract":"<div>Activities (mU larva−1) of enzymes critical to digestion were examined to better understand how newly-hatched (Zoea I) larvae of the mud crab Scylla serrata respond to intermittent food availability and food deprivation. Specifically, this study examined the activities of trypsin-like proteases, nonspecific esterases, and α-amylase across three experiments that simulated scenarios in which larvae hatch and experience rearing conditions where food was either: (1) continuously available or unavailable; (2) initially unavailable, but subsequently available; or (3) initially available, but subsequently unavailable. Results showed that food availability exerts a significant influence on enzyme profiles in newly-hatched larvae, with nutritional history influencing their response to food deprivation. When food was unavailable from hatch, there was no significant change in larval enzyme activities between 6 and 78 h post-hatch. If food became available at any point during this period, however, newly-hatched larvae were capable of rapidly (within 12–24 h) adjusting enzyme activities in response. Furthermore, a short (36 h) period of food availability appears sufficient to permit continuous substrate utilization during subsequent food deprivation of equivalent duration. Such flexibility is an important physiological strategy allowing newly-hatched larvae of S. serrata to adapt and thrive in challenging tropical oceanic environments and provides a basis for optimizing protocols for hatchery production of this species.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41220760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diacylglycerol acyltransferase (DGAT) in Crangon crangon and Pandalus montagui (Decapoda, Caridea) - Implications for lipid storage capacities and life history traits 桂根和熊猫的二酰基甘油酰基转移酶(DGAT) -对脂质储存能力和生活史性状的影响

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-10-01 DOI: 10.1016/j.cbpb.2023.110878

Marie Koch , Jan Tebben , Reinhard Saborowski

{"title":"Diacylglycerol acyltransferase (DGAT) in Crangon crangon and Pandalus montagui (Decapoda, Caridea) - Implications for lipid storage capacities and life history traits","authors":"Marie Koch , Jan Tebben , Reinhard Saborowski","doi":"10.1016/j.cbpb.2023.110878","DOIUrl":"10.1016/j.cbpb.2023.110878","url":null,"abstract":"<div>Lipids play essential roles in cell-structuring, cell-signaling, and as efficient metabolic energy stores. Lipid storage capacities determine life history traits of organisms and, thus, their ecological function. Among storage lipids, triacylglycerols (TAGs) are widespread in marine invertebrates. However, abilities to accumulate TAGs can vary even between closely related species, such as the caridean shrimps Crangon crangon and Pandalus montagui. The first species shows low TAG levels throughout the year in the main storage organ, the midgut gland, while the latter accumulates high TAG-levels, peaking in summer. TAGs synthesis is facilitated by the terminal step of the Kennedy-pathway, where the enzyme diacylglycerol-acyltransferase (DGAT) catalyzes the esterification of diacylglycerols with activated fatty acids. We investigated DGAT activity in the midgut gland using a fluorescent enzyme assay. Sequence information was extracted from whole transcriptome shotgun assembly data, that is publicly available on NCBI, and catalytic properties were deduced from molecular structure analysis. C. crangon showed significantly lower TAG synthesis rates than P. montagui, which explains the native TAG levels. Transcriptome data yielded several isoforms of DGAT enzymes in both species. C. crangon DGAT showed point mutations, which are capable of obstructing the catalytic capacity. The consequences are limited starvation resistance and, thus, presumably restricting C. crangon to a habitat with year-round sufficient food. In contrast, higher TAG synthesis rates presumably enable P. montagui to extend into northern subarctic habitats with limited food availability in winter. Moreover, the limited TAG synthesis and accumulation in the midgut gland may force C. crangon to direct energy into the ovaries, which results in multiple spawnings.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9978823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role of miR-8159-x in heat stress response in rainbow trout (Oncorhynchus mykiss) miR-8159-x在虹鳟(Oncorhynchus mykiss)热应激反应中的潜在作用

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-10-01 DOI: 10.1016/j.cbpb.2023.110877

Guiyan Zhao, Zhe Liu, Jinqiang Quan, Jun Sun, Lanlan Li, Junhao Lu

{"title":"Potential role of miR-8159-x in heat stress response in rainbow trout (Oncorhynchus mykiss)","authors":"Guiyan Zhao, Zhe Liu, Jinqiang Quan, Jun Sun, Lanlan Li, Junhao Lu","doi":"10.1016/j.cbpb.2023.110877","DOIUrl":"10.1016/j.cbpb.2023.110877","url":null,"abstract":"<div>Rainbow trout (Oncorhynchus mykiss) is a representative species of cold-water fish. Elevated temperatures during summer often result in significant high mortality rates. MicroRNAs (miRNAs) are class of small non-coding RNAs that play a crucial role as post-transcriptional regulators in various biological processes. Emerging evidence suggests that miRNAs are important regulators role during heat stress. Analyzing previously obtained miRNA-sequencing data, we observed substantial down regulation of miR-8159-x in the liver tissue of heat stressed rainbow trout. In this study, we conducted a dual luciferase reporter assay to validate that miR-8159-x target, a key gene involved in heat stress in rainbow trout. By examining the expression patterns of miR-8159-x and hsp90a1 in the liver tissue at 18 °C (CG) and 24 °C (HS) groups, we propose that miR-8159-x may negatively regulate hsp90a1. Furthermore, in vitro hepatocyte assay, transfection with miR-8159-x mimics significantly reduced the expression level of hsp90a1, whereas transfection with a miR-8159-x inhibitor yielded the opposite effect. Additionally, overexpression of miR-8159-x inhibited cell proliferation and induced apoptosis in normal rainbow trout hepatocytes. We further investigated the effects of miR-8159-x overexpression or inhibition on the mRNA and protein levels of the target gene hsp90a1 under heat stress conditions. In conclusion, our findings suggest that miR-8159-x participates in the biological response to heat stress by targeting hsp90a1. These results contribute to a better understanding of the molecular mechanisms underlying heat stress in rainbow trout and provide valuable insights for future research.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9972299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A kinetic characterization of the gill V(H+)-ATPase from two hololimnetic populations of the Amazon River shrimp Macrobrachium amazonicum 亚马逊河对虾两个全基因组群体鳃V（H+）-ATP酶的动力学特征

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-10-01 DOI: 10.1016/j.cbpb.2023.110880

Leonardo M. Fabri , Daniela P. Garçon , Cintya M. Moraes , Marcelo R. Pinto , John C. McNamara , Francisco A. Leone

{"title":"A kinetic characterization of the gill V(H+)-ATPase from two hololimnetic populations of the Amazon River shrimp Macrobrachium amazonicum","authors":"Leonardo M. Fabri , Daniela P. Garçon , Cintya M. Moraes , Marcelo R. Pinto , John C. McNamara , Francisco A. Leone","doi":"10.1016/j.cbpb.2023.110880","DOIUrl":"https://doi.org/10.1016/j.cbpb.2023.110880","url":null,"abstract":"<div>This investigation examines the kinetic characteristics and effect of acclimation to a brackish medium (21 ‰S) on gill V(H+)-ATPase activity in two hololimnetic populations of M. amazonicum. We also investigate the cellular immunolocalization of the enzyme. Immunofluorescence findings demonstrate that the V(H+)-ATPase c-subunit is distributed in the apical pillar cells of shrimps in fresh water but is absent after acclimation to 21 ‰S for 10 days. V(H+)-ATPase activity from the Tietê River population is ≈50% greater than the Grande River population, comparable to a wild population from the Santa Elisa Reservoir, but is 2-fold less than in cultivated shrimps. V(H+)-ATPase activity in the Tietê and the Grande River shrimps is abolished after 21 ‰S acclimation. The apparent affinities of the V(H+)-ATPase for ATP (0.27 ± 0.04 and 0.16 ± 0.03 mmol L−1, respectively) and Mg2+ (0.28 ± 0.05 and 0.14 ± 0.02 mmol L−1, respectively) are similar in both populations. The absence of V(H+)-ATPase activity in salinity-acclimated shrimps and its apical distribution in shrimps in fresh water underpins the importance of the crustacean V(H+)-ATPase for ion uptake in fresh water.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49794606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and functional characterization of two granulocyte colony stimulating factors in goldfish (Carassius auratus L.) 金鱼两种粒细胞集落刺激因子的分子及功能研究

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-10-01 DOI: 10.1016/j.cbpb.2023.110879

Moussa Gouife , Feiyang Wang , Ziqi Ban , Xinyuan Yue , Jianhu Jiang , Jiasong Xie

{"title":"Molecular and functional characterization of two granulocyte colony stimulating factors in goldfish (Carassius auratus L.)","authors":"Moussa Gouife , Feiyang Wang , Ziqi Ban , Xinyuan Yue , Jianhu Jiang , Jiasong Xie","doi":"10.1016/j.cbpb.2023.110879","DOIUrl":"10.1016/j.cbpb.2023.110879","url":null,"abstract":"<div>Granulocyte colony-stimulating factor (GCSF) is a member of the hematopoietic growth factor family that acts primarily on neutrophils and neutrophilic precursors to promote cell proliferation and differentiation. Although multiple GCSF genes have been found in teleosts, knowledge of their functions during fish hematopoietic development is still limited. Here, we report for the first time the molecular and functional characterization of two goldfish GCSFs (gfGCSF-a and gfGCSF-b). The open reading frame (ORF) of the gfGCSF-a and gfGCSF-b cDNA transcript consisted respectively of 624 bp and 678 bp with its ORF encoding 207 and 225 amino acids (aa), with a 17 aa signal peptide for each gene and a conserved domain of the IL-6 superfamily. Treatment of goldfish head kidney leukocytes (HKLs) with LPS increased gfGCSF-a and gfGCSF-b mRNA expression levels, also exposure of HKLs to either heat-killed or live A. hydrophila, induced transcriptional upregulation of gfGCSF-a and gfGCSF-b levels. Recombinant gfGCSF-a and gfGCSF-b protein (rgGCSF-a and rgGCSF-b) induced a dose-dependent production of TNFα and IL-1β from goldfish neutrophils. In vitro experiments showed rgGCSF-a and rgGCSF-b differentially promoted the proliferation and differentiation of leukocytes in goldfish. Furthermore, treatment of HKLs with rgGCSF-a showed significant upregulation of mRNA levels of the hematopoietic transcription factor GATA2, Runx1, MafB, and cMyb, while gfGCSF-b induces not only all four transcriptional factors mentioned above but also CEBPα. Our results indicate that goldfish GCSF-a and GCSF-b are important regulators of neutrophil proliferation and differentiation, which could stimulate different stages and lineages of hematopoiesis.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9972323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal expressions of nerve growth factor (NGF), and its receptor TrkA and p75 in the scent glands of muskrats (Ondatra zibethicus) 神经生长因子（NGF）及其受体TrkA和p75在大鼠气味腺中的季节性表达。

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-09-27 DOI: 10.1016/j.cbpb.2023.110905

Wenqian Xie, Qingjing Gao, Maria Daniela Artigas Ramirez, Haolin Zhang, Yuning Liu, Qiang Weng

{"title":"Seasonal expressions of nerve growth factor (NGF), and its receptor TrkA and p75 in the scent glands of muskrats (Ondatra zibethicus)","authors":"Wenqian Xie, Qingjing Gao, Maria Daniela Artigas Ramirez, Haolin Zhang, Yuning Liu, Qiang Weng","doi":"10.1016/j.cbpb.2023.110905","DOIUrl":"10.1016/j.cbpb.2023.110905","url":null,"abstract":"<div>NGF, also known as nerve growth factor, is crucial for the survival and differentiation of the nervous system, in addition to being involved in a number of non-neuronal systems. The aim of this work was to investigate the immunolocalization and expression patterns of NGF, its receptor, tyrosine kinase receptor A (TrkA), and p75 in the scent glands of muskrats (Ondatra zibethicus) throughout the breeding and non-breeding seasons. The scent gland mass showed considerable seasonal variations, with higher values during the breeding season and comparatively lower levels during the non-breeding season. While no immunostaining was observed in the interstitial cells, NGF, TrkA, and p75 were immunolocalized in the scent glandular cells and epithelial cells during both breeding and non-breeding seasons. NGF, TrkA, and p75 protein and mRNA expression levels were higher in the scent glands during breeding season compared to the non-breeding season. Circulating levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), and T in the scent gland were all significantly higher throughout the breeding season. The relative levels of the hormones in the plasma and the scent glands as well as NGF, TrkA, and p75 were positively associated with each other. Additionally, transcriptome analysis of the scent glands revealed that differentially expressed genes may be linked to steroid biosynthesis, the estrogen signaling pathway, and neurotransmitter transmembrane transporter function. These results suggest a potential role for NGF, TrkA, and p75 in controlling seasonal variations in the muskrats' scent gland functioning.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and expression analysis of Dmrt gene family and their role in gonad development of Pacific oyster (Crassostrea gigas) Dmrt基因家族的全基因组鉴定和表达分析及其在太平洋牡蛎性腺发育中的作用。

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-09-24 DOI: 10.1016/j.cbpb.2023.110904

Yetao Zeng , Haiqian Zheng , Cheng He , Chuanxu Zhang , Hongkuan Zhang , Huaiping Zheng

{"title":"Genome-wide identification and expression analysis of Dmrt gene family and their role in gonad development of Pacific oyster (Crassostrea gigas)","authors":"Yetao Zeng , Haiqian Zheng , Cheng He , Chuanxu Zhang , Hongkuan Zhang , Huaiping Zheng","doi":"10.1016/j.cbpb.2023.110904","DOIUrl":"10.1016/j.cbpb.2023.110904","url":null,"abstract":"<div>Doublesex and Mab-3-related transcription factor (Dmrt) is a type of transcription factor with a zinc-finger DM structural domain, which plays a significant role in sex determination and differentiation in animals. Although Dmrt has been reported in many vertebrates and invertebrates, it has rarely been studied in bivalves. In this study, a total of three members of the Dmrt gene family were identified and characterized in Crassostrea gigas, and all these CgDmrt genes contained a conserved DM domain. Analysis of the phylogenetic tree and gene structure revealed that Dmrt genes clustered on one branch may have similar functions in bivalves. Expression profiling of CgDmrt mRNA in different tissues and stages of gonad development indicated that CgDmrt3 exhibited sexually dimorphic expression and played an important role in the development of the male gonad in C. gigas. Furthermore, analysis of CgDmrt mRNA expression between fertile triploids and sterile triploids showed that CgDmrt3 may be involved in sperm production. Collectively, the systematic analysis of the CgDmrt genes will provide potential insights into the function of these genes in gonadal development.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, tissue specificity and regulation of expression of genes of four key enzymes related to long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis by ambient salinity during embryogenesis in the marine teleost Siganus guttatus 海洋硬骨鱼胚胎发生过程中环境盐度对长链多不饱和脂肪酸（LC-PUFA）生物合成相关四种关键酶基因的克隆、组织特异性和表达调控。

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-09-16 DOI: 10.1016/j.cbpb.2023.110903

Cuihong You , Shuangshuang Miao , Zhiyong Xie , Siyuan Lin , Shuqi Wang , Cuiying Chen , Li Lin , Yanhua Huang , Meng Zhou , Yewei Dong , Yuanyou Li , Ping Zhuang

{"title":"Cloning, tissue specificity and regulation of expression of genes of four key enzymes related to long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis by ambient salinity during embryogenesis in the marine teleost Siganus guttatus","authors":"Cuihong You , Shuangshuang Miao , Zhiyong Xie , Siyuan Lin , Shuqi Wang , Cuiying Chen , Li Lin , Yanhua Huang , Meng Zhou , Yewei Dong , Yuanyou Li , Ping Zhuang","doi":"10.1016/j.cbpb.2023.110903","DOIUrl":"10.1016/j.cbpb.2023.110903","url":null,"abstract":"<div>The rabbitfish Siganus canaliculatus was the first marine teleost reported to possess long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic ability, and the related molecular mechanisms have been well clarified. Here, we investigated the LC-PUFA biosynthesis capability of the congeneric rabbitfish S. guttatus. First, cDNAs of genes for four key enzymes related to LC-PUFA biosynthesis, namely Δ6Δ5 fatty acyl desaturase (fads2) (1335 bp; 445 aa), Δ4 fads2 (1335 bp; 445 aa), and elongation of very long chain fatty acid proteins (elovl5) (873 bp; 291 aa) and elovl4 (906 bp; 302 aa) were cloned from the liver of S. guttatus. The Δ6Δ5 fads2, Δ4 fads2 and elovl5 genes showed high expression in brain, liver, spleen, gallbladder, and intestine but relatively low expression in eye, whereas the elovl4 gene showed specific and high expression in eye. During embryogenesis, mRNA expression of Δ4 fads2 and elovl4 was detected from 8 h post-fertilization (hpf) and then maintained a high level to 24 hpf, while mRNA expression of Δ6Δ5 fads2 and elovl5 reached a peak at 14 hpf but then declined. In addition, ambient salinity (32 ppt and 20 ppt) exerted some regulatory influence on the expression of the four genes during embryogenesis. The levels of C18 PUFA precursors and, especially, PUFA and DHA of the embryos, decreased from 17 hpf to 24 hpf. These results suggested that S. guttatus, similar to the congeneric S. canaliculatus, would have capability for LC-PUFA biosynthesis, which is still not activated at the fertilized egg stage.</div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10634096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “pH evaluation in the digestive tract of the pygmy octopus, Paroctopus digueti” [Comparative Biochemistry and Physiology, Part B 269 (2024) 110881] “侏儒章鱼消化道pH值评估”的勘误表[比较生物化学和生理学，B部分269 (2024)110881]

IF 2.2 3区生物学

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology Pub Date : 2023-09-16 DOI: 10.1016/j.cbpb.2023.110897

N. Perales-García , D. Tovar-Ramírez , M.G. Martínez-Morales , B.P. Ceballos-Vázquez , D.A. Corona-Rojas , M.A. Salcedo-Meza , A. Garrido-Mora , F. Vega-Villasante , H. Nolasco-Soria

引用次数: 0