The c.503A>G polymorphism in ZIP1-II of Pacific oyster Crassostrea gigas associated with zinc content

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-18 DOI:10.1016/j.cbpb.2024.110988

Cong Luo , Ning Kong , Xiang Li , Shiqing Sun , Chunyu Jiang , Xin Qiao , Lingling Wang , Linsheng Song

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Abstract

The Pacific oyster Crassostrea gigas is renowned for its high zinc content, but the significant variation among individuals diminishes its value as a reliable source of zinc supplementation. The Zrt/Irt-like protein 1 (ZIP1), a pivotal zinc transporter that facilitates zinc uptake in various organisms, plays crucial roles in regulating zinc content. In the present study, polymorphisms of a ZIP1 gene in C. gigas (CgZIP1-II) were investigated, and their association with zinc content was evaluated through preliminary association analysis in 41 oysters and verification analysis in another 200 oysters. A total of 17 single nucleotide polymorphisms (SNPs) were identified in the exonic region of CgZIP1-II gene, with c.503A>G significantly associated with zinc content. Protein sequence and structure prediction showed that c.503A>G caused a p.Met110Val nonsynonymous mutation located in the metal-binding region of CgZIP1-II, which could influence its affinity for zinc ions, thereby modulating its zinc transport functionality. These results indicate the potential influence of CgZIP1-II polymorphisms on zinc content and provide candidate markers for selecting C. gigas with high zinc content.

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太平洋牡蛎（Crassostrea gigas）ZIP1-II 的 c.503A>G 多态性与锌含量的关系。

太平洋牡蛎（Crassostrea gigas）以锌含量高而闻名，但个体之间的显著差异降低了其作为可靠锌补充来源的价值。Zrt/Irt-like蛋白1（ZIP1）是一种关键的锌转运体，可促进多种生物对锌的吸收，在调节锌含量方面发挥着至关重要的作用。在本研究中，研究人员调查了千岛湖牡蛎 ZIP1 基因（CgZIP1-II）的多态性，并通过对 41 只牡蛎的初步关联分析和对另外 200 只牡蛎的验证分析，评估了它们与锌含量的关联。在 CgZIP1-II 基因的外显子区域共发现了 17 个单核苷酸多态性（SNPs），其中 c.503 A > G 与锌含量显著相关。蛋白质序列和结构预测显示，c.503 A > G导致了位于CgZIP1-II金属结合区的p.Met110Val非同义突变，这可能会影响其对锌离子的亲和力，从而改变其锌转运功能。这些结果表明了 CgZIP1-II 多态性对锌含量的潜在影响，并为挑选高锌含量的千层塔鱼提供了候选标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.