Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献

{"title":"Purslane leaf powder dietary supplementation rescues cadmium-induced disruption of behavior, antioxidant status, and expression of tight junction genes, in the brain of Nile tilapia (Oreochromis niloticus)","authors":"Nawal Alsubaie ,&nbsp;Yasmina M. Abd-Elhakim ,&nbsp;Amany Abdel-Rahman Mohamed ,&nbsp;Rowida E. Ibrahim ,&nbsp;Mohamed M.M. Metwally ,&nbsp;Tarek Khamis ,&nbsp;Alaa S. Alhegaili ,&nbsp;Abd Elhakeem El-Murr ,&nbsp;Badriyah S. Alotaibi ,&nbsp;Ahmed Abdulwahab Bawahab","doi":"10.1016/j.cbpb.2025.111086","DOIUrl":"10.1016/j.cbpb.2025.111086","url":null,"abstract":"<div><div>This study examined the effects of supplementing the Nile tilapia (<em>Oreochromis niloticus)</em> diet with purslane (<em>Portulaca oleracea</em> L.) leaf powder (PLNP, 10 g/kg) on neurobehavioral performance, brain oxidative status, tight junction mRNA expression, and brain histology in fish exposed to waterborne cadmium (Cd, 50 μg /L) for 60 days. Adding PLNP to the diet ameliorated the Cd-induced decline in ingestive behavior and swimming behavior, and reversed the Cd-induced increase in aggressive behavior. The significant decrease in the non-enzymatic (reduced glutathione) and enzymatic (catalase and superoxide dismutase) brain antioxidants detected in Cd-exposed fish was eliminated by dietary PLNP. PLNP supplementation also led to a decrease in brain malondialdehyde content, which was elevated by Cd exposure. In addition, dietary PLNP increased brain acetylcholinesterase content, upregulated mRNA expression of tight junction (<em>zo-2</em>, <em>claudin-4</em>, and <em>zo-1</em>) and oxidative stress genes (<em>sod-2</em>, <em>gpx</em>, and <em>nrf-2</em>), and downregulated apoptotic genes (<em>p53</em>, <em>caspase-9</em>, <em>caspase-8</em>, and <em>caspase-3</em>) in the brain, relative to the alterations in these parameters caused by Cd exposure. Furthermore, the Cd-induced histological changes in the Nile tilapia brain were ameliorated by PLNP dietary supplementation. In light of these findings, PLNP may be a useful dietary supplement for reducing the harmful effects of Cd on the brain and behavior of Nile tilapia.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111086"},"PeriodicalIF":1.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Docosapentaenoic (22:5 n-6) and docosahexaenoic (22:6 n-3) acids exhibit highly lipogenic properties in rainbow trout preadipocytes.","authors":"Gilles Tinant, Ineke Neefs, Alice De Groote, Melissa M Page, Jean-François Rees, Yvan Larondelle, Cathy Debier","doi":"10.1016/j.cbpb.2025.111087","DOIUrl":"https://doi.org/10.1016/j.cbpb.2025.111087","url":null,"abstract":"<p><p>Dietary polyunsaturated fatty acids are essential for fish health. Adipose tissue is the major tissue for fatty acid storage in rainbow trout (Oncorhynchus mykiss), and its development and function can be impacted by the fatty acids themselves. In the present study, the effects of seven fatty acids, oleic (OA, 18:1 n-9), α-linolenic (ALA, 18:3 n-3), eicosapentaenoic (EPA, 20:5 n-3), docosahexaenoic (DHA, 22:6 n-3), linoleic (LA, 18:2 n-6), arachidonic (AA, 20:4 n-6), and docosapentaenoic (DPA, 22:5 n-6) acids, on adipogenesis were investigated in primary cultures of rainbow trout preadipocytes. In terms of lipid accumulation, DPA and DHA appeared to be the most lipogenic fatty acids, while all treatments modified the fatty acid composition of the cellular phospholipids and neutral lipids. The fatty acid of interest added to the culture medium was the most abundant in preadipocytes, while the first bioconversion products were detected in lower amounts. In terms of transcriptional effects, DPA increased the expression of the early transcription factor CCAAT/enhancer binding protein δ, while DHA upregulated the expression of genes involved in neutral lipid synthesis, notably lipoprotein lipase, fatty acid transport protein 1 and glycerol-3-phosphate dehydrogenase. Both fatty acids decreased the expression of fatty acid synthase. These results highlight that DPA and DHA exert a significant effect on lipid deposition in rainbow trout preadipocytes, potentially through different pathways, and confirm that fatty acids have major impacts on preadipocyte lipid metabolism and adipogenesis.</p>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":" ","pages":"111087"},"PeriodicalIF":1.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary fatty acids on growth performance, oxidation status, fatty acid and phospholipid profiles of razor clam Sinonovacula constricta","authors":"Hailong Huang ,&nbsp;Yuxiang Zhu ,&nbsp;Kai Liao ,&nbsp;Yang Liu ,&nbsp;Yang Zhang ,&nbsp;Deshui Chen ,&nbsp;Bin Ma ,&nbsp;Jilin Xu","doi":"10.1016/j.cbpb.2025.111078","DOIUrl":"10.1016/j.cbpb.2025.111078","url":null,"abstract":"<div><div>Bivalves are a good source of lipid such as fatty acids and phospholipids, but little is known about how dietary fatty acids affect the fatty acid and phospholipid profiles of bivalves. In this study, the effects of dietary fatty acids on growth, oxidation status, fatty acid and phospholipid profiles of razor clams were investigated by employing microencapsulated feeds containing different oils (fish oil, FO; soybean oil, SO; flaxseed oil, FLO; coconut oil, CO; arachidonic acid-enriched oil, AO; olive oil, OO; and palm oil, PO). The results showed that the final weight of the razor clams fed FO enriched with eicosapentaenoic acid (EPA, C22:5n-3) and docosahexaenoic acid (DHA, C22:6n-3) was significantly higher than that of all other treatment groups. The final weight of the razor clams in the SO group was significantly higher than that in AO, OO and PO groups. The superoxide dismutase and catalase activity of the razor clams in the AO group was significantly higher than that in the OO group. Fatty acid profiles of the razor clams reflected dietary fatty acid composition. A total of 410 phospholipids were found in razor clams. Among them, phosphatidylcholine and phosphatidylethanolamine were the most abundant phospholipids. In addition, dietary fatty acids significantly altered the fatty acid composition of phospholipids. Our findings revealed that addition of certain amounts of EPA and DHA to the feed significantly promoted the growth rate of the razor clams. Moreover, the EPA and DHA content in the razor clams and their proportion in phospholipids increased significantly.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"278 ","pages":"Article 111078"},"PeriodicalIF":1.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The heat shock cognate protein 70 (HSC70) gene in the echiuran worm Urechis unicinctus: Cloning, tissue expression and response to sulfide exposure","authors":"Long Zhu ,&nbsp;Sijie Wang ,&nbsp;Ziyan Zhao ,&nbsp;Baiyu Li ,&nbsp;Chenxiao Xi ,&nbsp;Xinghong Xu","doi":"10.1016/j.cbpb.2025.111077","DOIUrl":"10.1016/j.cbpb.2025.111077","url":null,"abstract":"<div><div>To investigate the molecular characteristics of the heat shock cognate protein 70 (<em>HSC70</em>) gene in the echiuran worm <em>Urechis unicinctus</em>, the full-length complementary DNA (cDNA) sequence of <em>HSC70</em> in <em>U. unicinctus</em> was cloned and characterized. Additionally, the expression of <em>HSC70</em> in different tissues of <em>U. unicinctus</em> was detected after exposure to 0.25, 0.75, and 1.25 mg L⁻¹ Na₂S for 3, 6, 12, 24, 48, and 72 h, respectively. Results showed that the full-length cDNA of the <em>HSC70</em> gene was 2305 bp and encoded a deduced 658-amino acid (aa) protein. Three characteristic structural features of the <em>HSP70</em> gene family: IVLVGGSTRIPKIQK (residues 334–348), IFDLGGGTFDVSV (residues 197–210), and IDLGTTYSCV (residues 9–18), were identified in the HSC70 amino acid sequence. Multiple sequence alignment showed that <em>HSC70</em> was 87.25 % identical to <em>Meretrix meretrix</em> at the amino acid level, and phylogenetic analyses suggested that the <em>HSC70</em> gene of <em>U. unicinctus</em> was most closely grouped with <em>Urechis caupo</em>. Additionally, <em>HSC70</em> was expressed in all tested tissues, with the highest expression in hemolymph. <em>HSC70</em> mRNA expression was significantly increased after sulfide exposure, reaching a peak 3–6 h into the exposure, suggesting that <em>HSC70</em> expression was sensitive to environmental factors. Na₂S exposure for 12 h significantly increased the muscle content of HSC70, while a significant decrease was observed after Na₂S exposure for 72 h. Taken together, our study findings suggest that the <em>HSC70</em> gene is rapidly induced in <em>U. unicinctus</em> in response to sulfide exposure.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111077"},"PeriodicalIF":1.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Rhodopseudomonas palustris and composite probiotics on growth performance, intestinal health, and non-specific immunity of sea urchin (Strongylocentrotus intermedius)","authors":"Yuntian Zhang ,&nbsp;Rongwei Zhang ,&nbsp;Zhixu Guo ,&nbsp;Yi Chen ,&nbsp;Xiangyu Meng ,&nbsp;Yuzhe Han ,&nbsp;Xiaoran Zhao ,&nbsp;Tongjun Ren","doi":"10.1016/j.cbpb.2024.111058","DOIUrl":"10.1016/j.cbpb.2024.111058","url":null,"abstract":"<div><div>In recent decades, antibiotics have been widely used in sea urchin aquaculture to prevent diseases and improve water quality. However, their use leads to the emergence of resistant strains and environmental problems. Probiotics can serve as a green and pollution-free alternative. In this study, we mixed probiotics (<em>Rhodopseudomonas palustris</em>) (3 × 10⁸ CFU/mL) and composite probiotics (5 × 10⁸ CFU/mL) in a 1:1 ratio, establishing four supplementation levels: 0 % (FC, control group), 0.5 % (FL group), 1 % (FM group), and 2 % (FH group). At the end of the 50-day experiment, compared to the control group (FC), supplementation with <em>R. palustris</em> and composite probiotics (containing 60.13 % Lactobacillaceae and 20.79 % Acetobacteraceae) in the FL, FM, and FH groups significantly improved the growth performance of sea urchins (<em>Strongylocentrotus intermedius</em>), including final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), and gonadosomatic index (GSI). Additionally, antioxidant indicators (e.g., total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and catalase (CAT)), immune indicators (e.g., acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LZM)), and digestive enzyme activity were significantly increased in FM and FH groups. Probiotic supplementation also increased colonization of beneficial bacteria (Rhodobacteraceae) and enhanced microbiota diversity. The FM group, in particular, showed significantly upregulated expression of heat shock protein 70 (<em>HSP70</em>), glutathione peroxidase (<em>GPX</em>), glutathione (<em>GST</em>), toll-like receptor (<em>TLR</em>), and lysozyme (<em>LYZ</em>) genes. Overall, 1 % supplementation significantly enhanced growth, antioxidant capacity, digestive ability, microbiota stability, and immune-related gene expression in <em>S. intermedius</em>.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"276 ","pages":"Article 111058"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and functional analysis of a novel goose-type lysozyme from teleost Sebastes schlegelii with implications for antibacterial defense and immune cell modulation","authors":"Hao Jing ,&nbsp;Xue Yan ,&nbsp;Yue Wang ,&nbsp;Kai Yang ,&nbsp;Zi-yue Chen ,&nbsp;Guang-Hua Wang ,&nbsp;Min Zhang","doi":"10.1016/j.cbpb.2024.111060","DOIUrl":"10.1016/j.cbpb.2024.111060","url":null,"abstract":"<div><div>Lysozymes are crucial enzymes involved in the innate immune response against bacterial pathogens. In this study, we identified and characterized a goose-type lysozyme gene (<em>SsLyG</em>) from the black rockfish <em>Sebastes schlegelii</em>, an economically important aquaculture species. The deduced amino acid sequence of <em>SsLyG</em> contains 495 residues, which inculded a signal peptide, an immunoglobulin domain, and a goose egg-white lysozyme (GEWL) domain. Tissue expression analysis revealed the highest <em>SsLyG</em> levels in blood, and its transcription was significantly upregulated in the spleen and kidney upon bacterial and polyI:C challenges. Recombinant SsLyGE (rSsLyGE) exhibited lytic activity against <em>Micrococcus lysodeikticus</em> and concentration-dependent binding ability to <em>Staphylococcus aureus</em> and <em>Micrococcus luteus</em>. Furthermore, rSsLyGE promoted peripheral blood lymphocyte proliferation, enhanced macrophage respiratory burst activity, and increased reactive oxygen species production. RNA interference-mediated knockdown of <em>SsLyG</em> resulted in higher bacterial loads in the liver and spleen after <em>Listonella anguillarum</em> challenge, suggesting its role in early antibacterial defense. Collectively, these findings provide insights into the immune function of <em>SsLyG</em> and its potential application in developing antimicrobial strategies for aquaculture.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"276 ","pages":"Article 111060"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mitochondrial membrane potential and the sources of reactive oxygen species in the hemocytes of the ark clam Anadara kagoshimensis under hypoosmotic stress","authors":"Daria S. Lavrichenko,&nbsp;Elina S. Chelebieva,&nbsp;Ekaterina S. Kladchenko","doi":"10.1016/j.cbpb.2024.111057","DOIUrl":"10.1016/j.cbpb.2024.111057","url":null,"abstract":"<div><div>To compensate for changes in cell volume caused by changes in salt concentration, mollusks use regulatory mechanisms such as the regulation of volume decrease (RVD). This may increase the rate of aerobic metabolism and lead to an increase in reactive oxygen species (ROS). This study examined the production of ROS in the mitochondria of <em>Anadara kagoshiensis</em> hemocytes, the effect of mitochondrial inhibitors on osmotic stability in hemocytes, and the dynamics of changes in ROS levels and mitochondrial membrane potential when RVD is activated under hypo-osmotic conditions. Hemocytes maintained at a control osmolarity of 460 mOsm l⁻¹ showed significant decreases in ROS production following incubation with complex III inhibitors (S3QEL). Hypoosmotic shock stimulated RVD in all experimental groups. The cell volume increased by about 70 % immediately after osmolarity was reduced, and then decreased by about 40 % over the next 30 min. A reduction in osmolarity from about 460 to 200 mOsm l⁻¹ significantly decreased ROS and mitochondrial potentials in <em>A. kashimensis</em> hemocyctes. Inhibitors of mitochondrial complexes did not affect changes in ROS or mitochondria potentials in <em>A kashimiensis</em> hemocytes under hypoosmotic conditions or in hemocyte volume regulation mechanisms.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"276 ","pages":"Article 111057"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hermetia illucens larvae oil as an alternative lipid source: Effects on immune function, antioxidant activity, and inflammatory response in gilthead seabream juveniles","authors":"Sara Moutinho ,&nbsp;Helena Peres ,&nbsp;Filipa Fontinha ,&nbsp;Tássia Estevão-Rodrigues ,&nbsp;Óscar Monroig ,&nbsp;Rui Magalhães ,&nbsp;Lina Pulido-Rodríguez ,&nbsp;Giuliana Parisi ,&nbsp;Aires Oliva-Teles","doi":"10.1016/j.cbpb.2024.111059","DOIUrl":"10.1016/j.cbpb.2024.111059","url":null,"abstract":"<div><div><em>Hermetia illucens</em> larvae oil (HIO) is a promising new ingredient that can potentially be an alternative lipid source in aquafeeds. To assess its viability in gilthead seabream juvenile diets, a 10-week feeding trial was performed, and the effects on antioxidant, immune, and inflammatory responses were evaluated. Four diets were formulated to include HIO at increasing levels: 0, 4, 7.9, and 9.5 %, replacing a vegetable oil mix at 0, 42 %, 84 %, and 100 %, respectively. At the end of the trial, no significant changes were detected in the plasma immune humoral parameters, except for a linear increase in plasma peroxidase activity. Hepatic lipid peroxidation (LPO) remained unchanged, while the activity of antioxidant enzymes showed a linear increase corresponding to the level of dietary HIO inclusion. Fish fed the HIO diets exhibited lower intestinal LPO, and no differences between groups were observed in the activity of the oxidative stress-related enzymes. Regarding the inflammation-related genes, the different diets did not affect interleukin-1β and transforming growth factor β expressions in the intestine. In contrast, upregulation of tumor necrosis factor α and interleukin-10 was observed, being higher in fish fed the diet with total vegetable oil replacement than the others. In conclusion, these findings suggest that <em>Hermetia illucens</em> larvae oil can be included at levels up to 7.9 % of gilthead seabream juvenile diets without compromising their immune, antioxidant, and inflammatory responses while enhancing intestinal LPO.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"276 ","pages":"Article 111059"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporation of red macroalgae (Galaxaura oblongata) in roach (Rutilus rutilus) fingerling diet: Effects on growth, immunity, oxidative status and intestinal health.","authors":"Seyed Hossein Hoseinifar ,&nbsp;Mohammad Arghideh ,&nbsp;Fatemeh Hosseinpour Delavar ,&nbsp;Marina Paolucci ,&nbsp;Metin Yazici ,&nbsp;Mahsan Bayani ,&nbsp;Hien Van Doan","doi":"10.1016/j.cbpb.2025.111076","DOIUrl":"10.1016/j.cbpb.2025.111076","url":null,"abstract":"<div><div>This study investigates the effects of the red macroalgae <em>Galaxaura oblongata</em> diet supplementation on roach (<em>Rutilus rutilus</em>) fingerlings growth and gene expression related to immunity, the intestinal barrier, and antioxidant status. Roach fingerlings (2.26 ± 0.04 g) were fed a basic diet supplemented with three different percentages of <em>G. oblongata</em> powder: 0.25 % (G1), 0.50 % (G2), and 1.0 % (G3) over 8 weeks, with a control group (C) receiving no supplementation. The study found that growth parameters significantly increased in the G1 and G2 groups compared to the C and G3 groups (<em>P</em> &lt; 0.05). Immune responses, measured by total immunoglobulin (Ig) and lysozyme activity, showed a significant increase in the whole-body extract of the G2 group (P &lt; 0.05) and the skin mucus of all treated groups compared to the control (P &lt; 0.05). <em>G. oblongata</em> supplementation did not significantly affect catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities in the whole-body extract, although GPx activity in skin mucus was significantly higher in the supplemented groups. Additionally, the gene expression of interleukin 1-beta (<em>il-1β)</em>, <em>occludin</em>, <em>gpx</em> and <em>sod</em>, but not <em>Toll-like receptor</em> increased in <em>G. oblongata</em> treated groups. These results suggest that <em>G. oblongata</em> can serve as a beneficial feed additive in the culture of roach fingerlings, enhancing growth and immune function.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111076"},"PeriodicalIF":1.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, molecular characterization and expression patterns of Cathepsin L in Yesso scallop (Patinopecten yessoensis) shell-infested by Polydora","authors":"Yunna Hang ,&nbsp;Hongyan Sun ,&nbsp;Anqi Tang,&nbsp;Xinxin Fan ,&nbsp;Ying Tian,&nbsp;Xubo Wang,&nbsp;Chen Jiang,&nbsp;Junxia Mao,&nbsp;Zhenlin Hao,&nbsp;Jun Ding,&nbsp;Yaqing Chang","doi":"10.1016/j.cbpb.2025.111075","DOIUrl":"10.1016/j.cbpb.2025.111075","url":null,"abstract":"<div><div><em>Patinopecten yessoensis</em> (Yesso scallop), one of the most important aquaculture molluscs in China, has recently suffered severe <em>Polydora</em> disease, causing economic losses. Cathepsin L (CatL), a cysteine protease, has important functions in immune responses in vertebrates and invertebrates. However, little is known regarding the structure and function of <em>CatL</em> in scallops. In this study, a <em>CatL</em> gene named <em>PyCatL</em> was first identified in the genome of <em>P. yessoensis</em>. Gene structure analysis of <em>PyCatL</em> revealed it had 8 exons and 7 introns and a full length of 7916 bp. The gene sequence was analysed, and typically conserved functional domains (signal peptide, inhibitor I29 domain, and peptidase C1 domain) and motifs (ERWNIN, GNYD and GCXGG) of CatL were all predicted in PyCatL, confirming the sequence as belonging to a <em>CatL</em> gene. Phylogenetic analysis showed the evolutionary status of CatL was consistent with the species taxonomy. <em>PyCatL</em> was expressed ubiquitously in all the tested tissues in this study, suggesting its involvement in a wide range of physiological processes. After <em>Polydora</em> infestation, <em>PyCatL</em> exhibited significant upregulation in various mantle regions at both mRNA and protein levels, contrasting with a notable decrease in gene expression in hemocytes. Additionally, the enzyme activity of PyCatL showed a significant increase in the mantle of diseased <em>P. yessoensis</em>. The results suggested a role for mantle tissue in response to <em>Polydora</em> infestation by upregulating expression of <em>PyCatL</em>. The study offers novel insights into the function of <em>CatL</em> in innate immunity in scallops.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"277 ","pages":"Article 111075"},"PeriodicalIF":1.9,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}