The energy metabolism of the freshwater leech Whitmania pigra in response to fasting

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-07 DOI:10.1016/j.cbpb.2024.110999

Jiali Liu , Panpan Jin , Mingyue Li , Xiaozhe Yi , Yu Tian , Zhaolei Zhang , Jinxin Liu , Linchun Shi

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引用次数: 0

Abstract

Non-blood-feeding leeches, Whitmania pigra, have evolved unique digestive structures and physiological mechanisms to cope with fasting. However, the metabolic changes and molecular mechanisms induced by fasting remain unclear. Therefore, this study recorded the weights of leeches during the fasting process. The weight changes were divided into two stages: a rapid decline period (1–9 weeks) and a fluctuating decline period (9–24 weeks). Leeches fasted for 4 (H4), 11 (H11), and 24 (H24) weeks were selected for transcriptome sequencing. Compared to the control group (H0), 436, 1157, and 337 differentially expressed genes (DEGs) were identified, which were mainly related to glycolysis/gluconeogenesis, amino acid metabolism, and the lipid metabolism pathway. The 6-phosphofructokinase (Pfk), pyruvate kinase (PK), and phosphoenolpyruvate carboxykinase (Pck) transcription levels revealed glycolysis/gluconeogenesis activation during the early stage of fasting and peaked at 11 weeks. Decreased expression of the rate-limiting enzyme acetyl-CoA carboxylase (ACC) in fatty acid synthesis during fasting may impede fatty acid synthesis. These results indicated that the nutrient storage and energy-supplying pathways in W. pigra were modified to improve fasting resistance. The findings of this study provided guidance for exploring the mechanism underlying fasting metabolism and laid a foundation for artificial breeding to improve the resistance of leeches.

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淡水水蛭 Whitmania pigra 对禁食的能量代谢反应。

不吸血的水蛭（Whitmania pigra）进化出了独特的消化结构和生理机制来应对禁食。然而，禁食引起的代谢变化和分子机制仍不清楚。因此，本研究记录了水蛭在禁食过程中的体重。体重变化分为两个阶段：快速下降期（1-9 周）和波动下降期（9-24 周）。选择禁食 4 周（H4）、11 周（H11）和 24 周（H24）的水蛭进行转录组测序。与对照组（H0）相比，分别发现了 436、1157 和 337 个差异表达基因（DEGs），这些基因主要与糖酵解/糖元生成、氨基酸代谢和脂质代谢途径有关。6-磷酸果糖激酶（Pfk）、丙酮酸激酶（PK）和磷酸烯醇丙酮酸羧激酶（Pck）的转录水平显示，糖酵解/糖元生成在禁食早期被激活，并在11周时达到峰值。禁食期间，脂肪酸合成的限速酶乙酰-CoA羧化酶（ACC）的表达量减少，可能会阻碍脂肪酸的合成。这些结果表明，猪笼草营养储存和能量供应途径的改变提高了猪笼草的耐空腹性。该研究结果为探索水蛭禁食代谢机制提供了指导，并为人工繁殖提高水蛭抗性奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.