Current Topics in Developmental Biology最新文献_第4页

The interplay between retinoic acid binding proteins and retinoic acid degrading enzymes in modulating retinoic acid concentrations. 视黄酸结合蛋白和视黄酸降解酶在调节视黄酸浓度方面的相互作用。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2024-10-24 DOI: 10.1016/bs.ctdb.2024.09.001

Nina Isoherranen, Yue Winnie Wen

{"title":"The interplay between retinoic acid binding proteins and retinoic acid degrading enzymes in modulating retinoic acid concentrations.","authors":"Nina Isoherranen, Yue Winnie Wen","doi":"10.1016/bs.ctdb.2024.09.001","DOIUrl":"10.1016/bs.ctdb.2024.09.001","url":null,"abstract":"The active metabolite of vitamin A, all-trans-retinoic acid (atRA), is critical for maintenance of many cellular processes. Although the enzymes that can synthesize and clear atRA in mammals have been identified, their tissue and cell-type specific roles are still not fully established. Based on the plasma protein binding, tissue distribution and lipophilicity of atRA, atRA partitions extensively to lipid membranes and other neutral lipids in cells. As a consequence, free atRA concentrations in cells are expected to be exceedingly low. As such mechanisms must exist that allow sufficiently high atRA concentrations to occur for binding to retinoic acid receptor (RARs) and for RAR mediated signaling. Kinetic simulations suggest that cellular retinoic acid binding proteins (CRABPs) provide a cytosolic reservoir for atRA to allow high enough cytosolic concentrations that enable RAR signaling. Yet, the different CRABP family members CRABP1 and CRABP2 may serve different functions in this context. CRABP1 may reside in the cytosol as a member of a cytosolic signalosome and CRABP2 may bind atRA in the cytosol and localize to the nucleus. Both CRABPs appear to interact with the atRA-degrading cytochrome P450 (CYP) family 26 enzymes in the endoplasmic reticulum. These interactions, together with the expression levels of the CRABPs and CYP26s, likely modulate cellular atRA concentration gradients and tissue atRA concentrations in a tightly coordinated manner. This review provides a summary of the current knowledge of atRA distribution, metabolism and protein binding and how these characteristics may alter tissue atRA concentrations.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"161 ","pages":"167-200"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early retinoic acid signaling organizes the body axis and defines domains for the forelimb and eye. 早期维甲酸信号组织身体轴并定义前肢和眼睛的区域。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2024-11-08 DOI: 10.1016/bs.ctdb.2024.10.002

Gregg Duester

{"title":"Early retinoic acid signaling organizes the body axis and defines domains for the forelimb and eye.","authors":"Gregg Duester","doi":"10.1016/bs.ctdb.2024.10.002","DOIUrl":"10.1016/bs.ctdb.2024.10.002","url":null,"abstract":"All-trans RA (ATRA) is a small molecule derived from retinol (vitamin A) that directly controls gene expression at the transcriptional level by serving as a ligand for nuclear ATRA receptors. ATRA is produced by ATRA-generating enzymes that convert retinol to retinaldehyde (retinol dehydrogenase; RDH10) followed by conversion of retinaldehyde to ATRA (retinaldehyde dehydrogenase; ALDH1A1, ALDH1A2, or ALDH1A3). Determining what ATRA normally does during vertebrate development has been challenging as studies employing ATRA gain-of-function (RA treatment) often do not agree with genetic loss-of-function studies that remove ATRA via knockouts of ATRA-generating enzymes. In mouse embryos, ATRA is first generated at stage E7.5 by ATRA-generating enzymes whose genes are first expressed at that stage. This article focuses upon what ATRA normally does at early stages based upon these knockout studies. It has been observed that early-generated ATRA performs three essential functions: (1) activation of genes that control hindbrain and spinal cord patterning; (2) repression of Fgf8 in the heart field and caudal progenitors to provide an FGF8-free region in the trunk essential for somitogenesis, heart morphogenesis, and initiation of forelimb fields; and (3) actions that stimulate invagination of the optic vesicle to form the optic cup.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"161 ","pages":"1-32"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterning the nephron: Forming an axial polarity with distal and proximal specialization. 肾元模式：形成远端和近端特化的轴向极性。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-01-28 DOI: 10.1016/bs.ctdb.2025.01.005

Nils Olof Lindström, Jessica May Vanslambrouck

引用次数: 0

Vitamin A supply in the eye and establishment of the visual cycle. 维生素A在眼睛的供应和视觉周期的建立。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2024-10-02 DOI: 10.1016/bs.ctdb.2024.09.003

Sepalika Bandara, Johannes von Lintig

引用次数: 0

Renal ciliopathies. 肾ciliopathies。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-03-18 DOI: 10.1016/bs.ctdb.2025.01.009

Laura A Devlin, Rebecca M Dewhurst, Praveen D Sudhindar, John A Sayer

引用次数: 0

Ion channels and transporters involved in calcium flux regulation in mammalian sperm. 哺乳动物精子中钙通量调节的离子通道和转运体。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-03-13 DOI: 10.1016/bs.ctdb.2025.01.006

Claudia Sánchez-Cárdenas, Enrique I Oliver, Julio C Chávez, Guillermina M Luque, Arturo Hernández-Cruz, Mariano G Buffone, Alberto Darszon, Pablo E Visconti, Ana Romarowski

引用次数: 0

Human kidney organoids for modeling the development of different diseases. 人类肾脏类器官用于模拟不同疾病的发展。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-02-06 DOI: 10.1016/bs.ctdb.2024.12.001

Elena Ceccotti, Armina Semnani, Benedetta Bussolati, Stefania Bruno

{"title":"Human kidney organoids for modeling the development of different diseases.","authors":"Elena Ceccotti, Armina Semnani, Benedetta Bussolati, Stefania Bruno","doi":"10.1016/bs.ctdb.2024.12.001","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2024.12.001","url":null,"abstract":"The increasing incidence of kidney diseases has highlighted the need for in vitro experimental models to mimic disease development and to test new therapeutic approaches. Traditional two-dimensional in vitro experimental models are not fully able to recapitulate renal diseases. Instead, kidney organoids represent three-dimensional models that better mimic the human organ from both structural and functional points of view. Human pluripotent stem cells (PSCs), both embryonic and induced, are ideal sources for generating renal organoids. These organoids contain all renal cell types and the protocols to differentiate PSCs into renal organoids consist of three different stages that recapitulate embryonic development: mesodermal induction, nephron progenitor formation, and nephron differentiation. Recently it has been establish a renal organoid model where collecting ducts are also present. In this case, the presence of ureteric bud progenitor cells is essential. Renal organoids are particularly useful for studying genetic diseases, by introducing the specific mutations in PSCs by genome editing or generating organoids from patient-derived PSCs. Moreover, renal organoids represent promising models in toxicology studies and testing new therapeutic approaches. Renal organoids can be established also from adult stem cells. This type of organoid, named tubuloid, is composed only of epithelial cells and recapitulates the tissue repair process. The tubuloids can be generated from adult stem or progenitor cells, obtained from renal biopsies or urine, and are promising in vitro models for studying tubular functions, diseases, and regeneration. Tubuloids can be derived from patients and permit the study of genetic diseases, performing personalized drug screening and modeling renal pathologies.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"163 ","pages":"364-393"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo. 没有转录，没有问题：蛋白磷酸化的改变和从卵母细胞到胚胎的转变。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-02-18 DOI: 10.1016/bs.ctdb.2025.01.001

Jonathon M Thomalla, Mariana F Wolfner

{"title":"No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo.","authors":"Jonathon M Thomalla, Mariana F Wolfner","doi":"10.1016/bs.ctdb.2025.01.001","DOIUrl":"10.1016/bs.ctdb.2025.01.001","url":null,"abstract":"Although mature oocytes are arrested in a differentiated state, they are provisioned with maternally-derived macromolecules that will start embryogenesis. The transition to embryogenesis, called 'egg activation', occurs without new transcription, even though it includes major cell changes like completing stalled meiosis, translating stored mRNAs, cytoskeletal remodeling, and changes to nuclear architecture. In most animals, egg activation is triggered by a rise in free calcium in the egg's cytoplasm, but we are only now beginning to understand how this induces the egg to transition to totipotency and proliferation. Here, we discuss the model that calcium-dependent protein kinases and phosphatases modify the phosphorylation landscape of the maternal proteome to activate the egg. We review recent phosphoproteomic mass spectrometry analyses that revealed broad phospho-regulation during egg activation, both in number of phospho-events and classes of regulated proteins. Our interspecies comparisons of these proteins pinpoints orthologs and protein families that are phospho-regulated in activating eggs, many of which function in hallmark events of egg activation, and others whose regulation and activity warrant further study. Finally, we discuss key phospho-regulating enzymes that may act apically or as intermediates in the phosphorylation cascades during egg activation. Knowing the regulators, targets, and effects of phospho-regulation that cause an egg to initiate embryogenesis is crucial at both fundamental and applied levels for understanding female fertility, embryo development, and cell-state transitions.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"162 ","pages":"165-205"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From neural crest migration to the onset of gangliogenesis. 从神经嵴迁移到神经节发生的开始。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-03-13 DOI: 10.1016/bs.ctdb.2025.02.003

Hugo A Urrutia, Marianne E Bronner

{"title":"From neural crest migration to the onset of gangliogenesis.","authors":"Hugo A Urrutia, Marianne E Bronner","doi":"10.1016/bs.ctdb.2025.02.003","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2025.02.003","url":null,"abstract":"The neural crest is a highly migratory and multipotent cell population that contributes to many defining features of vertebrates. As a uniquely vertebrate cell type, the neural crest is an excellent model for studying cell lineage and diversification during embryonic development because of its multipotency, motility, and capacity to form a plethora of derivatives. Neural crest cells migrate extensively throughout the body and contribute to many of the defining features of vertebrate embryos, including the craniofacial skeleton, most of the peripheral nervous system and pigmentation of the skin. What guides their migration and subsequent formation of discrete structures? Interactions between neural crest cells and their environment, including other cell types like placode cells, play a major role in guiding their migration and condensation into numerous derivatives. In this review, we discuss aspects of neural crest induction, migration and axial level differences, highlighting what is currently known regarding molecular cues that govern their formation, migratory behavior, and differentiation as they reach their final destinations. We particularly focus on formation of cranial sensory ganglia. New technologies are playing an important role in furthering our understanding of the molecular mechanisms underlying neural crest migration and what leads to cessation of their movement and onset of differentiation.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"164 ","pages":"67-108"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside influences: The impact of extracellular matrix mechanics on cell migration. 外界影响：细胞外基质力学对细胞迁移的影响。

2区生物学

Current Topics in Developmental Biology Pub Date : 2025-01-01 Epub Date: 2025-01-28 DOI: 10.1016/bs.ctdb.2025.01.003

Ronen Zaidel-Bar, Priti Agarwal

{"title":"Outside influences: The impact of extracellular matrix mechanics on cell migration.","authors":"Ronen Zaidel-Bar, Priti Agarwal","doi":"10.1016/bs.ctdb.2025.01.003","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2025.01.003","url":null,"abstract":"\"No cell is an island\" - highlights the interconnectedness of cellular behavior and the extracellular matrix (ECM). Cell migration is inherently contextual, as cells navigate and adapt to their environments, reshaping the ECM while being influenced by its properties. This review focuses on the mechanical characteristics of the ECM-specifically its architecture, porosity, dynamics, and stiffness-and how these attributes affect cell behavior and migration strategies. We discuss how the mechanical properties are modulated by the composition and arrangement of ECM components and the role of enzymatic activities, including crosslinking and matrix metalloproteinases. By presenting examples from vertebrate and invertebrate developmental models, we demonstrate how ECM mechanics dictate cell migration at various biological scales. Additionally, we examine the importance of cell-matrix adhesions in regulating migration speed and direction. While in vitro studies have advanced our understanding of the molecular mechanisms at play, significant questions persist regarding the regulation of cell migration by ECM mechanics in vivo. Ultimately, this synthesis aims to illuminate the complexities of cell-ECM mechanical interactions, pointing the way for future research that may unveil novel insights into how ECM mechanics influences cell migration during development and disease.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"164 ","pages":"29-65"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0