Current Topics in Developmental Biology最新文献_第4页

Protein synthesis during mouse spermatogenesis. 小鼠精子发生过程中的蛋白质合成。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-01-22 DOI: 10.1016/bs.ctdb.2026.01.001

Lele Yang, Kun Hou, Tin-Lap Lee, Huayu Qi

{"title":"Protein synthesis during mouse spermatogenesis.","authors":"Lele Yang, Kun Hou, Tin-Lap Lee, Huayu Qi","doi":"10.1016/bs.ctdb.2026.01.001","DOIUrl":"10.1016/bs.ctdb.2026.01.001","url":null,"abstract":"Spermatogenesis is composed of three consecutive stages, mitosis, meiosis and spermiogenesis, during which spermatogenic cells undergo continuous molecular and cellular transformation. Regulation of gene expression has been underlying major molecular mechanisms that govern the progressive cell fate determination during spermatogenesis. Besides epigenetic and transcriptional controls, the un-coupled transcription and translation is a common phenomenon conserved across phyla during spermatogenesis. Translational regulation determines the dynamic proteomic landscapes in spermatogenic cells. Aberrant protein synthesis caused by mutations in RNA binding proteins (RBPs) and translation regulators often cast detrimental effects on spermatogenesis in a stage-specific manner, leading to male infertility. Regulation of gene expression at the post-transcriptional and translational levels bear advantages of fast and flexible responses to changing environment, coordination of cellular states including energy and nutrient availability, preservation of genomic fidelity and quantitative and qualitative control of proteins, the functional units of the cell. How cell type-specific translation is regulated during spermatogenesis is largely unclear. In this review, we will first introduce general features of protein synthesis and what have been revealed during mouse spermatogenesis when aberrant protein synthesis occurred. We will then analyze the differential signaling pathways and intracellular factors that cooperatively regulate proteomic landscapes in spermatogenic cells at various stages of spermatogenesis. Protein synthesis is a fundamental mechanism underlying cell fate determination. Taking advantage of current advancements in methodology, future research in this area will unveil the design principles that govern how cells program and maintain functional proteome during development and disease.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"168 ","pages":"371-428"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From syncytium to sperm: Coordinated membrane and cytoskeletal remodeling in Drosophila spermiogenesis. 从合胞体到精子：果蝇精子发生中协调的膜和细胞骨架重塑。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-03-27 DOI: 10.1016/bs.ctdb.2026.03.005

Carihann Dominicci-Cotto, Andreas Jenny

{"title":"From syncytium to sperm: Coordinated membrane and cytoskeletal remodeling in Drosophila spermiogenesis.","authors":"Carihann Dominicci-Cotto, Andreas Jenny","doi":"10.1016/bs.ctdb.2026.03.005","DOIUrl":"10.1016/bs.ctdb.2026.03.005","url":null,"abstract":"Male infertility has increased in the recent past, with declining sperm counts affecting many couples. Critically, its genetic and cellular causes remain only partially understood. Contributing to male sterility are defects in sperm production, structure, function, and seminal fluid quality, with sperm abnormalities also negatively impacting pregnancy and fetal development. Spermatogenesis is a highly complex, multistep process requiring germline-somatic interactions and the coordination of cell division with the remodeling of the cytoskeleton and with enormous growth of membranes. Regulated by a plethora of genes, spermatogenesis is particularly vulnerable to genetic and epigenetic disruptions. These complexities make the identification and functional validation of genes orchestrating male gametogenesis particularly challenging. Model organisms such as Drosophila melanogaster provide powerful systems to study gene function, and this review thus focuses on spermiogenesis in flies, the post-meiotic process with the most extensive structural changes necessary for the production of functional sperm.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"168 ","pages":"317-370"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell competition. 干细胞竞争。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-03-30 DOI: 10.1016/bs.ctdb.2026.03.001

Erika A Bach

引用次数: 0

Simply complex - the structure of the cerebellar neuronal lineage. 简单的复杂——小脑神经元谱系的结构。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-02-14 DOI: 10.1016/bs.ctdb.2026.01.012

Thomas Butts, Richard James T Wingate

{"title":"Simply complex - the structure of the cerebellar neuronal lineage.","authors":"Thomas Butts, Richard James T Wingate","doi":"10.1016/bs.ctdb.2026.01.012","DOIUrl":"10.1016/bs.ctdb.2026.01.012","url":null,"abstract":"With its limited diversity of neuronal types and stereotyped cellular organisation, the cerebellum is an excellent model for complex brain development. It exemplifies how simple patterning rules can give rise to complex neural circuits. The entirety of populations of excitatory and inhibitory neurons is characterised by the transient expression of either Atonal1 (Atoh1) or Ptf1a, respectively, and derived from a spatially defined population of Sox2-positive precursors. We present a model where the decision to make Atoh1 over Ptf1a lineage neurons is dictated by inductive cell-cell interactions at the posterior boundary with non-neural roof plate cells at the rhombic lip. The type of Atoh1+ve or Ptf1a+ve cell generated is dictated by a shared temporal code invested in the Sox2-expressing progenitor pool in the ventricular zone of dorsal rhombomere 1. An additional long-lived pool of Sox2 progenitors in the prospective white matter gives rise to glial cells (astrocytes) and later born interneurons, the latter of which also transiently express Ptf1a. Temporal patterning of progenitors generates neuronal diversity and offers a potent substrate for adaptation. In particular, fine-grained temporal patterning of progenitors feeding early rhombic lip derivatives dictates the connections of the cerebellum through specifying cerebellar nucleus output neurons which influence the scaling of the cortex of the cerebellum. In the human cerebellum, scaling involves species-specific adaptations that co-evolved within the human cerebral cortex.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"167 ","pages":"1-31"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mediator at the Helm: Coordinating transcription and biomolecular condensates in hematopoiesis. 掌舵的介质：造血过程中协调转录和生物分子凝聚。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-08-26 DOI: 10.1016/bs.ctdb.2026.06.002

Shicong Zhu, Yiyang Zhang, Hsiang-Ying Lee

{"title":"Mediator at the Helm: Coordinating transcription and biomolecular condensates in hematopoiesis.","authors":"Shicong Zhu, Yiyang Zhang, Hsiang-Ying Lee","doi":"10.1016/bs.ctdb.2026.06.002","DOIUrl":"https://doi.org/10.1016/bs.ctdb.2026.06.002","url":null,"abstract":"Hematopoiesis relies on precisely coordinated transcriptional programs that balance stem cell self-renewal, lineage commitment, and terminal differentiation. Central to this regulation is the Mediator complex, a large multi-subunit transcriptional co-regulator that integrates signals from transcription factors and chromatin regulators to control RNA polymerase Ⅱ (Pol Ⅱ) activity. The dynamic and modular composition of Mediator enables context-dependent transcriptional outputs, while individual subunits can exert specialized regulatory functions during hematopoietic lineage specification, thereby contributing to cell-fate-specific transcriptional outputs. Recent advances further reveal that transcriptional regulation is shaped by the spatial organization of regulatory machinery with biomolecular condensates formed through liquid-liquid phase separation (LLPS), particularly at super-enhancers. In this emerging framework, Mediator functions not only as a transcriptional integrator but also as a key coordinator of transcriptional machinery within condensates at cell-fate-related gene loci. In this chapter, we summarize how distinct Mediator subunits confer specific modes of transcriptional regulation and discuss how the interplay between Mediator and phase-separated condensates shapes transcriptional control during hematopoiesis. We highlight how specific subunits, including MED1 and MED26, participate in distinct regulatory modes in erythropoiesis, spanning super-enhancer-driven transcriptional activation, progenitor expansion, and condensate-associated mechanisms that influence Pol Ⅱ pausing and global transcription repression during terminal differentiation. Together, these findings support a model in which Mediator integrates transcriptional regulation with nuclear organization through condensate-mediated mechanisms, providing a conceptual framework for understanding hematopoietic cell fate decisions and transcriptional dysregulation in hematological diseases.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"170 ","pages":"87-123"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148992268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaling and regeneration in the developing cerebellum. 发育中的小脑的鳞片和再生。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-02-09 DOI: 10.1016/bs.ctdb.2026.01.006

Jens Bager Christensen, Giada Vanacore, Niamh Divers, N Sumru Bayin

{"title":"Scaling and regeneration in the developing cerebellum.","authors":"Jens Bager Christensen, Giada Vanacore, Niamh Divers, N Sumru Bayin","doi":"10.1016/bs.ctdb.2026.01.006","DOIUrl":"10.1016/bs.ctdb.2026.01.006","url":null,"abstract":"The cerebellum is a hindbrain structure that houses the majority of neurons in the mammalian brain and is involved in motor coordination, balance and higher cognitive processes. Compared to the rest of the brain, cerebellar development is protracted, with neurogenesis continuing postnatally. While the local circuitry of the cerebellum is relatively simple, having the correct proportions of its various cell types is essential for cerebellar function. Remarkably, the neonatal mouse cerebellum is highly regenerative upon injury at birth, utilizing context- and age-dependent regenerative strategies to ensure robust and efficient repair. Here, we provide an overview of how different lineages within the cerebellum interact during development and discuss the mechanisms of scaling, where the earlier-born neurons regulate the survival and proportional expansion of other cell types. We give examples of disorders where the scaling could be disrupted, perinatal injuries that affect the cerebellum and potential regenerative mechanisms in the brain. Furthermore, we discuss how the neonatal cerebellum regenerates the correct cell types in the appropriate proportions. Finally, we summarize the cell-intrinsic and -extrinsic mechanisms that regulate progenitor behaviors during postnatal cerebellar development and regeneration, specifically focusing on the nestin-expressing progenitors of the postnatal cerebellum.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"167 ","pages":"267-299"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synaptonemal complex: The structural basis for meiosis I. 突触复合体：减数分裂的结构基础。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-02-20 DOI: 10.1016/bs.ctdb.2026.01.014

Hanwei Jiang, Suixing Fan, Qinghua Shi

{"title":"Synaptonemal complex: The structural basis for meiosis I.","authors":"Hanwei Jiang, Suixing Fan, Qinghua Shi","doi":"10.1016/bs.ctdb.2026.01.014","DOIUrl":"10.1016/bs.ctdb.2026.01.014","url":null,"abstract":"As a specialized form of cell division for the generation of haploid gametes, meiosis is characterized by the separation of homologous chromosomes in meiosis I. Two key events take place in meiotic prophase I to ensure the proper segregation of homologous chromosomes, including the formation of the synaptonemal complex (SC) and the initiation and repair of programmed DNA double-strand breaks (DSBs). Both of these events are essential for the generation of crossovers. The SC serves as the structural basis of all the chromosome behaviors in meiotic prophase I. It not only reorganizes the chromosomes into a highly ordered stem-loop structure and holds the homologous chromosomes together when DSBs are being repaired, but also works as a platform for the recruitment of proteins playing essential roles in the formation and repair of DSBs, as well as the checkpoint monitoring the proper meiosis progression. Given the critical role of the SC in meiosis, questions concerning its structure and function are well studied in the field of meiosis research. Here, we not only reviewed the studies concerning the assembly of the SC, the function of the SC during male meiosis, and how mutations related to the SC coding genes affect male fertility, but also summarized the critical questions to be solved in future research.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"168 ","pages":"245-279"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current understanding of adult stem cells in Leydig cell biology. 间质细胞生物学对成体干细胞的最新认识。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2026-03-31 DOI: 10.1016/bs.ctdb.2026.03.002

Yiyan Wang, Xiaoheng Li, Huitao Li, Ren-Shan Ge

{"title":"Current understanding of adult stem cells in Leydig cell biology.","authors":"Yiyan Wang, Xiaoheng Li, Huitao Li, Ren-Shan Ge","doi":"10.1016/bs.ctdb.2026.03.002","DOIUrl":"10.1016/bs.ctdb.2026.03.002","url":null,"abstract":"Adult Leydig cells (ALCs) are specialized androgen-producing cells that originate from stem Leydig cells (SLCs) during pubertal development. SLCs reside in the testicular interstitial compartment, either centrally or near peritubular myoid cells and vasculature. While their precise origin remains under investigation, prevailing evidence suggests SLCs derive from mesonephros-derived mesenchymal cells, though contributions from neural crest or coelomic epithelium lineages have been proposed. Luteinizing hormone (LH) is a critical regulator of Leydig cell maturation and steroidogenic function, yet the mechanisms enabling SLCs to acquire responsiveness to LH/growth factor signals remain incompletely characterized. Current models suggest paracrine factors from Sertoli cells, peritubular myoid cells, immune cells, or germ cells may orchestrate the early SLC-to-Leydig cell transition prior to LH sensitivity. Fetal Leydig cell-derived androgens may also prime ALC development. Postnatally, LH signaling drives both proliferative expansion (yielding ∼25 million Leydig cells in adult rats) and terminal differentiation of intermediate progenitor stages, ultimately establishing the testosterone-secreting ALC population essential for male reproductive function.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"168 ","pages":"163-209"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the intestine: The role of cell morphology and spatial dynamics in development. 肠的形成：细胞形态和空间动力学在发育中的作用。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 Epub Date: 2025-11-14 DOI: 10.1016/bs.ctdb.2025.10.002

Yuying Wang, Zhang Wen, Kaelyn Sumigray

{"title":"Shaping the intestine: The role of cell morphology and spatial dynamics in development.","authors":"Yuying Wang, Zhang Wen, Kaelyn Sumigray","doi":"10.1016/bs.ctdb.2025.10.002","DOIUrl":"10.1016/bs.ctdb.2025.10.002","url":null,"abstract":"To optimize nutrient absorption and protect against physical, chemical, and microbial threats, the small intestine undergoes extensive remodeling during embryogenesis and postnatal development to establish peristalsis, expand absorptive surface area, and modulate epithelial turnover. Each developmental stage demands precise spatiotemporal regulation of cell fate specification and positioning while simultaneously coordinating cell shape changes to drive organogenesis. This chapter examines mammalian small intestinal morphogenesis from late embryogenesis through postnatal maturation, highlighting the interplay between epithelial-mesenchymal signaling, intrinsic actomyosin dynamics, and external mechanical forces in gut tube elongation, smooth muscle patterning, villus morphogenesis, and crypt formation. Once the crypt-villus axis is established, epithelial cells employ dynamic extracellular matrix interactions and cytoskeletal reorganization to migrate toward the villus tip, where they are extruded to maintain high turnover. Insights from animal models and in vitro organoid systems reveal how tissue architecture not only emerges from but reinforces epithelial maturation and functional specialization.","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"166 ","pages":"67-99"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147488706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. 前言。

2区生物学

Current Topics in Developmental Biology Pub Date : 2026-01-01 DOI: 10.1016/S0070-2153(26)00061-X

Huayu Qi

引用次数: 0