Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology最新文献

{"title":"Essential and nonessential fatty acid composition and use in overwintering monarch butterflies.","authors":"Libesha Anparasan, Matthias Pilecky, M Isabel Ramirez, Keith A Hobson, Martin J Kainz, Leonard I Wassenaar","doi":"10.1007/s00359-024-01727-0","DOIUrl":"https://doi.org/10.1007/s00359-024-01727-0","url":null,"abstract":"<p><p>Monarch butterflies (Danaus plexippus) undertake one of the most remarkable long-distance insect migrations, travelling thousands of kilometres to overwinter in the central trans-volcanic belt of Mexico. This study explored how monarch butterflies use essential fatty acids (EFA) and nonessential fatty acids (NFA) during overwintering. We collected 150 (male/female) butterflies from the Sierra Chincua wintering colony from the time of arrival (December 2022) to before departure (February 2023) and analysed their lipid content. Our findings revealed that although females have a higher mass fraction of lipids, male and female monarch butterflies depleted their lipids similarly over time, resulting in low abdominal lipid mass fractions by late February. NFA, including oleic and palmitic acid, were predominantly used for energy during overwintering by male and female butterflies. In contrast, the EFA alpha-linolenic and linoleic acids, critical for reproductive success and cellular functions, were conserved in both sexes. Males began the overwintering period with a higher mass fraction of EFA in the polar components of the head and thorax, which may impact the degree of cold acclimation of these tissues during this period. Strategic lipid utilisation, prioritising the preservation of EFA over NFA and optimizing overwintering survival probably enhance readiness for spring remigration and reproduction. This differential fatty acid use underscores the delicate balance monarch butterflies maintain to survive overwintering and highlights the potential impacts of environmental changes on their lipid dynamics and survival.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vector-based navigation in desert ants: the significance of path-integration vectors.","authors":"Beatrice Voegeli, Stefan Sommer, Markus Knaden, Rüdiger Wehner","doi":"10.1007/s00359-024-01725-2","DOIUrl":"https://doi.org/10.1007/s00359-024-01725-2","url":null,"abstract":"<p><p>In the longstanding discussion of whether insects, especially central place foragers such as bees and ants, use metric representations of their landmark surroundings (so-called \"cognitive maps\"), the ability to find novel shortcuts between familiar locations has been considered one of the most decisive proofs for the use of such maps. Here we show by channel-based field experiments that desert ants Cataglyphis can travel such shortcuts between locations (defined by memorized goal vectors) just on the basis of path integration. When trained to visit two spatially separated feeders A and B they later travel the hitherto novel route A→B. This behavior may originate from the interaction of goal vectors retrieved from long-term memory and the current vector computed by the continuously running path integrator. Based on former experiments, we further argue that path integration is a necessary requirement also for acquiring landmark information (in form of learned goal-directed views). This emphasizes the paramount importance of path integration in these central place foragers. Finally we hypothesize that the ant's overall system of navigation consists in the optimal combination of path-integration vectors and view-based vectors, and thus handles and uses vectorial information without the need of constructing a \"vector map\", in which vectors are linked to known places in the environment others than to the origin of all journeys, the nest.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do wild-caught fly larvae cooperatively forage?","authors":"Rives Kuhar, Madeline Williamson, Peyton Yee, Guzel Naik, Sean Michael Cursain, Barry Condron","doi":"10.1007/s00359-024-01724-3","DOIUrl":"https://doi.org/10.1007/s00359-024-01724-3","url":null,"abstract":"<p><p>Animals often form organized cooperative foraging groups, where individual members must adhere to specific rules to maintain cohesiveness. These groups face the challenge of managing potential intruders, who may or may not assist in foraging. In semi-liquid food environments, Drosophila larvae learn to synchronize their movements into clusters, which are thought to make feeding more efficient. Individuals who do not synchronize with the group are excluded from the cluster. Whether clustering behavior occurs in wild-caught larvae, and if so, the extent of their selectivity in group membership, remains unknown. Here, we show that clustering occurs across a number of fly species, and the capacity to join different clusters varies both between and within species. We collected and observed a larval cluster from rotting fruit in the field, yielding seven fly species. Subsequent tests for clustering on five lines from this collection and 20 other inbred wild-caught lines revealed that all species, except D. suzukii, exhibit clustering behavior. Each line demonstrates varying capacities to become members of different clusters. This study also indicates that there is high genetic variance in how individual lines cluster with each other that is not explained by cross species features. Additionally, combinations of wild species with lab benchmark strains give varied outcomes in resultant adult fitness. The ability to co-cluster varies between and within species boundaries. However, fly lines that cluster with another tend to impart fitness both to themselves and their host. Our findings demonstrate that multiple species of fly larvae can co-cluster. This behavior tends to confer mutual benefits to cluster members, suggesting significant ecological implications in Drosophila communities.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel nerve regeneration assessment method using adult zebrafish with crush spinal cord injury.","authors":"Hiroaki Motohashi, Satoshi Sugita, Yoshito Hosokawa, Takahiro Hasumura, Shinichi Meguro, Noriyasu Ota, Yoshihiko Minegishi","doi":"10.1007/s00359-024-01723-4","DOIUrl":"https://doi.org/10.1007/s00359-024-01723-4","url":null,"abstract":"<p><p>Zebrafish (Danio rerio), an alternative to rodents, are widely used in neurological, genetic, and toxicology research. The zebrafish larval spinal cord injury model has been used in neural mechanistic analyses owing to its high regenerative capacity and throughput; however, it also had several limitations in imitating rodents. Therefore, we investigated the use of adult zebrafish as an alternative model to rodents for evaluating nerve regeneration. Here, we established a novel spinal cord regeneration evaluation method, which was based on the maximum swimming speed of adult zebrafish in a custom-built hydrodynamic-based aquarium. The spinal cords of adult male zebrafish were crushed using forceps, and maximum swimming speed and histological spinal cord regeneration were evaluated. Spinal cord-injured zebrafish showed a significant decline in motor function, followed by recovery at 3 weeks postoperatively, accompanied by histological regeneration. Spinal cord regeneration can be indirectly assessed by monitoring maximum swimming speed. They were also fed a diet containing fig extract, which can promote peripheral nerve regeneration; they were fed daily starting 1 week before the operation. Maximum swimming speed was measured time-dependently until 3 weeks postoperatively. Fig-consuming fish showed improved recovery of maximum swimming speed compared to the controls, which was consistent with the histological analysis. In summary, we established a spinal cord regeneration assessment system using adult zebrafish in a customized aquarium, which enables researchers to evaluate spinal cord regeneration in adult zebrafish similar to that of rodent experiments, contributing to faster and easier screening of neuroregenerative technology.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shelter selection in females of two scorpion species depends on shelter size and scent.","authors":"Janina Hladik, Yorick Bailer, Harald Wolf, Torben Stemme","doi":"10.1007/s00359-024-01721-6","DOIUrl":"https://doi.org/10.1007/s00359-024-01721-6","url":null,"abstract":"<p><p>Shelter selection is an important task in an animal's life. Concerning scorpions, little is known on the evaluation of potential shelters and the importance of chemosensation. To address these issues, we conducted a two-choice shelter test in rectangular open field arenas to identify properties rendering shelters attractive for female scorpions of the species E. italicus and M. eupeus. Shelters varied in size (large, small) and scent (none, conspecific: male or female, attractive: prey, aversive: rosemary oil). Contact with the shelters was video-recorded under red light for 13 h, including the whole night phase. Results revealed a preference for larger shelters, with conspecific scent having minor or no influence. Striking differences occurred with regard to prey and rosemary oil scents. Prey scent was more attractive to M. eupeus, while rosemary oil did not act as a repellent. E. italicus was not very attracted by prey scent, but was repelled by rosemary oil. These findings might reflect the different habitats, semi-arid vs. Mediterranean climates: prey and rosemary are scarce in the semi-arid climate (habitat of M. eupeus), whereas they are abundant in the Mediterranean climate (habitat of E. italicus). We carried out impairment experiments to identify the main sensory organs responsible for the above observations. These are the pectines and pedipalps which function as mechano- and chemosensors. Scorpions could not detect size nor scent properly when either their pectines or pedipalps were impaired.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A versatile recording device for the analysis of continuous daily external activity in colonies of highly eusocial bees.","authors":"Arthur Roque Justino, Klaus Hartfelder","doi":"10.1007/s00359-024-01709-2","DOIUrl":"10.1007/s00359-024-01709-2","url":null,"abstract":"<p><p>As pollinators, bees are key to maintaining the biodiversity of angiosperm plants, and for agriculture they provide a billion-dollar ecosystem service. But they also compete for resources (primarily nectar and pollen), especially the highly social bees that live in perennial colonies. So, how do they organize their daily temporal activities? Here, we present a versatile, low-cost device for the continuous, automatic recording and data analysis of the locomotor activity in the colony-entrance tube of highly eusocial bees. Consisting of an in-house built block containing an infrared detector, the passage of bees in the colony entrance tunnel is registered and automatically recorded in an Arduino environment, together with concomitant recordings of temperature and relative humidity. With a focus on the highly diverse Neotropical stingless bees (Meliponini), we obtained 10-day consecutive recordings for two colonies each of the species Melipona quadrifasciata and Frieseomelitta varia, and also for the honey bee. The Lomb-Scargle periodogram analysis identified a predominant circadian rhythmicity for all three species, but also indications of ultradian rhythms. For M. quadrifasciata, which is comparable in size to the honey bee, we found evidence for a possibly anticipatory activity already before sunrise. As all three species also presented activity at night in the colony entrance tube, this also raises questions about sleep organization in social insects. The cost and versatility of the device and the open-source options for data analysis make this an attractive system for conducting studies on circadian rhythms in social bees under natural conditions, complementing studies on flower visits by these important pollinators.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":"885-900"},"PeriodicalIF":1.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141428354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of pre-diapause phase in the northern Drosophila species D. ezoana.","authors":"Koustubh M Vaze, Giulia Manoli, Charlotte Helfrich-Förster","doi":"10.1007/s00359-024-01707-4","DOIUrl":"10.1007/s00359-024-01707-4","url":null,"abstract":"<p><p>Drosophila ezoana is a virilis group Drosophila species inhabiting northern latitudes. The flies enter adult reproductive diapause to survive winter upon exposure to short photoperiod conditions (short-day) over several consecutive days. Insect pre-diapause phase - the duration between the beginning of exposure to short days and expression of diapause is thought to be comprised of two distinct phases - (a) photoperiodic time measurement that detects short-days, followed by (b) physiological events leading to the expression of diapause phenotype. A short-day dependent segment of the pre-diapause phase thus approximates the process of photoperiodic time measurement. Continuous darkness has been found to be a neutral condition with respect to diapause regulation in many insect species. The effect of variable number of short-days followed by continuous darkness on diapause incidence thus allows identification of short-day dependent segment of pre-diapause phase thereby mapping the process of photo-periodic time measurement. Although, few weeks of exposure to short-days in adult stage is known to be sufficient for the expression of diapause in D. ezoana, the number of short days required for the completion of photo-periodic time measurement has never been systematically analysed. Our experiments show that continuous darkness is a neutral condition for diapause regulation also in D. ezoana. We utilized the neutral nature of continuous darkness to map the process of photoperiodic time measurement in the D. ezoana strain 124OJ8 which showed that integration of short-day photic cues over the first 10 days of pre-diapause phase is essential for diapause induction.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":"901-908"},"PeriodicalIF":1.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141447644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of doxapram and its potential interactions with K2P channels in experimental model preparations.","authors":"Elizabeth R Elliott, Kaitlyn E Brock, Rachael M Vacassenno, Douglas A Harrison, Robin L Cooper","doi":"10.1007/s00359-024-01705-6","DOIUrl":"10.1007/s00359-024-01705-6","url":null,"abstract":"<p><p>The channels commonly responsible for maintaining cell resting membrane potentials are referred to as K2P (two-P-domain K⁺ subunit) channels. These K⁺ ion channels generally remain open but can be modulated by their local environment. These channels are classified based on pharmacology, pH sensitivity, mechanical stretch, and ionic permeability. Little is known about the physiological nature of these K2P channels in invertebrates. Acidic conditions depolarize neurons and muscle fibers, which may be caused by K2P channels given that one subtype can be blocked by acidic conditions. Doxapram is used clinically as a respiratory aid known to block acid-sensitive K2P channels; thus, the effects of doxapram on the muscle fibers and synaptic transmission in larval Drosophila and crawfish were monitored. A dose-dependent response was observed via depolarization of the larval Drosophila muscle and an increase in evoked synaptic transmission, but doxapram blocked the production of action potentials in the crawfish motor neuron and had a minor effect on the resting membrane potential of the crawfish muscle. This indicates that the nerve and muscle tissues in larval Drosophila and crawfish likely express different K2P channel subtypes. Since these organisms serve as physiological models for neurobiology and physiology, it would be of interest to further investigate what types of K2P channel are expressed in these tissues. (212 words).</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":"869-884"},"PeriodicalIF":1.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141159270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptually salient differences in a species recognition cue do not promote auditory streaming in eastern grey treefrogs (Hyla versicolor).","authors":"Lata Kalra, Shoshana Altman, Mark A Bee","doi":"10.1007/s00359-024-01702-9","DOIUrl":"10.1007/s00359-024-01702-9","url":null,"abstract":"<p><p>Auditory streaming underlies a receiver's ability to organize complex mixtures of auditory input into distinct perceptual \"streams\" that represent different sound sources in the environment. During auditory streaming, sounds produced by the same source are integrated through time into a single, coherent auditory stream that is perceptually segregated from other concurrent sounds. Based on human psychoacoustic studies, one hypothesis regarding auditory streaming is that any sufficiently salient perceptual difference may lead to stream segregation. Here, we used the eastern grey treefrog, Hyla versicolor, to test this hypothesis in the context of vocal communication in a non-human animal. In this system, females choose their mate based on perceiving species-specific features of a male's pulsatile advertisement calls in social environments (choruses) characterized by mixtures of overlapping vocalizations. We employed an experimental paradigm from human psychoacoustics to design interleaved pulsatile sequences (ABAB…) that mimicked key features of the species' advertisement call, and in which alternating pulses differed in pulse rise time, which is a robust species recognition cue in eastern grey treefrogs. Using phonotaxis assays, we found no evidence that perceptually salient differences in pulse rise time promoted the segregation of interleaved pulse sequences into distinct auditory streams. These results do not support the hypothesis that any perceptually salient acoustic difference can be exploited as a cue for stream segregation in all species. We discuss these findings in the context of cues used for species recognition and auditory streaming.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":"853-867"},"PeriodicalIF":1.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Same same, but different: exploring the enigmatic role of the pituitary adenylate cyclase-activating polypeptide (PACAP) in invertebrate physiology.","authors":"Zsolt Pirger, Péter Urbán, Bence Gálik, Bence Kiss, Antal Tapodi, János Schmidt, Gábor K Tóth, Joris M Koene, György Kemenes, Dóra Reglődi, Tibor Kiss, István Fodor","doi":"10.1007/s00359-024-01706-5","DOIUrl":"10.1007/s00359-024-01706-5","url":null,"abstract":"<p><p>Evidence has been accumulating that elements of the vertebrate pituitary adenylate cyclase-activating polypeptide (PACAP) system are missing in non-chordate genomes, which is at odds with the partial sequence-, immunohistochemical-, and physiological data in the literature. Multilevel experiments were performed on the great pond snail (Lymnaea stagnalis) to explore the role of PACAP in invertebrates. Screening of neuronal transcriptome and genome data did not reveal homologs to the elements of vertebrate PACAP system. Despite this, immunohistochemical investigations with an anti-human PAC₁ receptor antibody yielded a positive signal in the neuronal elements in the heart. Although Western blotting of proteins extracted from the nervous system found a relevant band for PACAP-38, immunoprecipitation and mass spectrometric analyses revealed no corresponding peptide fragments. Similarly to the effects reported in vertebrates, PACAP-38 significantly increased cAMP synthesis in the heart and had a positive ionotropic effect on heart preparations. Moreover, it significantly modulated the effects of serotonin and acetylcholine. Homologs to members of Cluster B receptors, which have shared common evolutionary origin with the vertebrate PACAP receptors, PTHRs, and GCGRs, were identified and shown not to be expressed in the heart, which does not support a potential role in the mediation of PACAP-induced effects. Our findings support the notion that the PACAP system emerged after the protostome-deuterostome divergence. Using antibodies against vertebrate proteins is again highlighted to have little/no value in invertebrate studies. The physiological effects of vertebrate PACAP peptides in protostomes, no matter how similar they are to those in vertebrates, should be considered non-specific.</p>","PeriodicalId":54862,"journal":{"name":"Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology","volume":" ","pages":"909-925"},"PeriodicalIF":1.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}