Journal of Heredity最新文献_第6页

Divergent ecological selection maintains species boundaries despite gene flow in a rare endemic tree, Quercus acerifolia (maple-leaf oak). 尽管枫叶栎（Quercus acerifolia）是一种罕见的特有树种，但在其基因流动过程中，不同的生态选择仍能维持物种边界。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-08-20 DOI: 10.1093/jhered/esae033

Yingtong Wu, Alexander G Linan, Sean Hoban, Andrew L Hipp, Robert E Ricklefs

{"title":"Divergent ecological selection maintains species boundaries despite gene flow in a rare endemic tree, Quercus acerifolia (maple-leaf oak).","authors":"Yingtong Wu, Alexander G Linan, Sean Hoban, Andrew L Hipp, Robert E Ricklefs","doi":"10.1093/jhered/esae033","DOIUrl":"10.1093/jhered/esae033","url":null,"abstract":"Strong gene flow from outcrossing relatives tends to blur species boundaries, while divergent ecological selection can counteract gene flow. To better understand how these two forces affect the maintenance of species boundaries, we focused on a species complex including a rare species, maple-leaf oak (Quercus acerifolia), which is found in only four disjunct ridges in Arkansas. Its limited range and geographic proximity to co-occurring close relatives create the possibility for genetic swamping. In this study, we gathered genome-wide single nucleotide polymorphisms (SNPs) using restriction-site-associated DNA sequencing (RADseq) from 190 samples of Q. acerifolia and three of its close relatives, Q. shumardii, Q. buckleyi, and Q. rubra. We found that Q. shumardii and Q. acerifolia are reciprocally monophyletic with low support, suggesting incomplete lineage sorting, introgression between Q. shumardii and Q. acerifolia, or both. Analyses that model allele distributions demonstrate that admixture contributes strongly to this pattern. Populations of Q. acerifolia experience gene flow from Q. shumardii and Q. rubra, but we found evidence that divergent selection is likely maintaining species boundaries: 1) ex situ collections of Q. acerifolia have a higher proportion of hybrids compared to the mature trees of the wild populations, suggesting ecological selection against hybrids at the seed/seedling stage; 2) ecological traits co-vary with genomic composition; and 3) Q. acerifolia shows genetic differentiation at loci hypothesized to influence tolerance of radiation, drought, and high temperature. Our findings strongly suggest that in maple-leaf oak, selection results in higher divergence at regions of the genome despite gene flow from close relatives.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"575-587"},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141332532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic assessment within a species complex of a subterranean rodent (Geomys bursarius) with conservation implications for isolated subspecies. 地下啮齿动物（Geomys bursarius）物种群内部的系统发育评估，以及对孤立亚种的保护意义。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-08-20 DOI: 10.1093/jhered/esae035

Nathan Alexander, Alida de Flamingh, Bradley J Cosentino, Robert L Schooley

{"title":"Phylogenetic assessment within a species complex of a subterranean rodent (Geomys bursarius) with conservation implications for isolated subspecies.","authors":"Nathan Alexander, Alida de Flamingh, Bradley J Cosentino, Robert L Schooley","doi":"10.1093/jhered/esae035","DOIUrl":"10.1093/jhered/esae035","url":null,"abstract":"Range contraction and expansion from glaciation have led to genetic divergence that may be particularly pronounced in fossorial species with low dispersal. The plains pocket gopher (Geomys bursarius) is a fossorial species that ranges widely across North America but has a poorly understood phylogeny. We used mitogenomes (14,996 base pairs) from 56 individuals across seven subspecies, plus two outgroup species, to assess genetic divergence from minimum spanning trees, measure genetic distances, and infer phylogenetic trees using BEAST. We found G. b. wisconsinensis was monophyletic with recent divergence. Further assessment is needed for G. b. major because it was paraphyletic and exhibited inconsistent groupings with other clades. Importantly, we identified G. b. illinoensis as being genetically distinct and monophyletic likely due to a unique colonization event eastward across the Mississippi River. Because G. b. illinoensis faces continued pressures from niche reduction and habitat loss, we recommend that G. b. illinoensis be considered an evolutionary significant unit warranting conservation actions to promote connectivity and restore suitable habitat. Such conservation efforts should benefit other grassland species including those originating from clades west of the Mississippi River that may also be evolutionary significant units.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"565-574"},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genome assembly of the American black bear, Ursus americanus, from California. 加利福尼亚州美洲黑熊的基因组组装。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-08-20 DOI: 10.1093/jhered/esae037

Megan A Supple, Merly Escalona, Jillian Adkins, Michael R Buchalski, Nicolas Alexandre, Ruta M Sahasrabudhe, Oanh Nguyen, Samuel Sacco, Colin Fairbairn, Eric Beraut, William Seligmann, Richard E Green, Erin Meredith, Beth Shapiro

{"title":"A genome assembly of the American black bear, Ursus americanus, from California.","authors":"Megan A Supple, Merly Escalona, Jillian Adkins, Michael R Buchalski, Nicolas Alexandre, Ruta M Sahasrabudhe, Oanh Nguyen, Samuel Sacco, Colin Fairbairn, Eric Beraut, William Seligmann, Richard E Green, Erin Meredith, Beth Shapiro","doi":"10.1093/jhered/esae037","DOIUrl":"10.1093/jhered/esae037","url":null,"abstract":"The American black bear, Ursus americanus, is a widespread and ecologically important species in North America. In California, the black bear plays an important role in a variety of ecosystems and serves as an important species for recreational hunting. While research suggests that the populations in California are currently healthy, continued monitoring is critical, with genomic analyses providing an important surveillance tool. Here we report a high-quality, near chromosome-level genome assembly from a U. americanus sample from California. The primary assembly has a total length of 2.5 Gb contained in 316 scaffolds, a contig N50 of 58.9 Mb, a scaffold N50 of 67.6 Mb, and a BUSCO completeness score of 96%. This U. americanus genome assembly will provide an important resource for the targeted management of black bear populations in California, with the goal of achieving an appropriate balance between the recreational value of black bears and the maintenance of viable populations. The high quality of this genome assembly will also make it a valuable resource for comparative genomic analyses among black bear populations and among bear species.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"498-506"},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analyses of the banded alder borer (Rosalia funebris) and Asian longhorned beetle (Anoplophora glabripennis) genomes reveal significant differences in genome architecture and gene content among these and other Cerambycidae. 对带状桤木螟（Rosalia funebris）和亚洲长角甲虫（Anoplophora glabripennis）基因组的比较分析表明，这些甲虫和其他角虫的基因组结构和基因含量存在显著差异。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-08-20 DOI: 10.1093/jhered/esae021

Terrence Sylvester, Richard Adams, Robert F Mitchell, Ann M Ray, Rongrong Shen, Na Ra Shin, Duane D McKenna

{"title":"Comparative analyses of the banded alder borer (Rosalia funebris) and Asian longhorned beetle (Anoplophora glabripennis) genomes reveal significant differences in genome architecture and gene content among these and other Cerambycidae.","authors":"Terrence Sylvester, Richard Adams, Robert F Mitchell, Ann M Ray, Rongrong Shen, Na Ra Shin, Duane D McKenna","doi":"10.1093/jhered/esae021","DOIUrl":"10.1093/jhered/esae021","url":null,"abstract":"Rosalia funebris (RFUNE; Cerambycidae), the banded alder borer, is a longhorn beetle whose larvae feed on the wood of various economically and ecologically significant trees in western North America. Adults are short-lived and not known to consume plant material substantially. We sequenced, assembled, and annotated the RFUNE genome using HiFi and RNASeq data. We documented genome architecture and gene content, focusing on genes putatively involved in plant feeding (phytophagy). Comparisons were made to the well-studied genome of the Asian longhorned beetle (AGLAB; Anoplophora glabripennis) and other Cerambycidae. The 814 Mb RFUNE genome assembly was distributed across 42 contigs, with an N50 of 30.18 Mb. Repetitive sequences comprised 60.27% of the genome, and 99.0% of expected single-copy orthologous genes were fully assembled. We identified 12,657 genes, fewer than in the four other species studied, and 46.4% fewer than for Aromia moschata (same subfamily as RFUNE). Of the 7,258 orthogroups shared between RFUNE and AGLAB, 1,461 had more copies in AGLAB and 1,023 had more copies in RFUNE. We identified 240 genes in RFUNE that putatively arose via horizontal transfer events. The RFUNE genome encoded substantially fewer putative plant cell wall degrading enzymes than AGLAB, which may relate to the longer-lived plant-feeding adults of the latter species. The RFUNE genome provides new insights into cerambycid genome architecture and gene content and provides a new vantage point from which to study the evolution and genomic basis of phytophagy in beetles.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"516-523"},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into the critically endangered King Island scrubtit. 从基因组学角度了解极度濒危的国王岛恙虫病。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-08-20 DOI: 10.1093/jhered/esae029

Ross Crates, Brenton von Takach, Catherine M Young, Dejan Stojanovic, Linda E Neaves, Liam Murphy, Daniel Gautschi, Carolyn J Hogg, Robert Heinsohn, Phil Bell, Katherine A Farquharson

{"title":"Genomic insights into the critically endangered King Island scrubtit.","authors":"Ross Crates, Brenton von Takach, Catherine M Young, Dejan Stojanovic, Linda E Neaves, Liam Murphy, Daniel Gautschi, Carolyn J Hogg, Robert Heinsohn, Phil Bell, Katherine A Farquharson","doi":"10.1093/jhered/esae029","DOIUrl":"10.1093/jhered/esae029","url":null,"abstract":"Small, fragmented, or isolated populations are at risk of population decline due to fitness costs associated with inbreeding and genetic drift. The King Island scrubtit Acanthornis magna greeniana is a critically endangered subspecies of the nominate Tasmanian scrubtit A. m. magna, with an estimated population of <100 individuals persisting in three patches of swamp forest. The Tasmanian scrubtit is widespread in wet forests on mainland Tasmania. We sequenced the scrubtit genome using PacBio HiFi and undertook a population genomic study of the King Island and Tasmanian scrubtits using a double-digest restriction site-associated DNA (ddRAD) dataset of 5,239 SNP loci. The genome was 1.48 Gb long, comprising 1,518 contigs with an N50 of 7.715 Mb. King Island scrubtits formed one of four overall genetic clusters, but separated into three distinct subpopulations when analyzed independently of the Tasmanian scrubtit. Pairwise FST values were greater among the King Island scrubtit subpopulations than among most Tasmanian scrubtit subpopulations. Genetic diversity was lower and inbreeding coefficients were higher in the King Island scrubtit than all except one of the Tasmanian scrubtit subpopulations. We observed crown baldness in 8/15 King Island scrubtits, but 0/55 Tasmanian scrubtits. Six loci were significantly associated with baldness, including one within the DOCK11 gene which is linked to early feather development. Contemporary gene flow between King Island scrubtit subpopulations is unlikely, with further field monitoring required to quantify the fitness consequences of its small population size, low genetic diversity, and high inbreeding. Evidence-based conservation actions can then be implemented before the taxon goes extinct.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"552-564"},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141181523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Limited evidence for extensive genetic differentiation between X and Y chromosomes in Hybognathus amarus (Cypriniformes: Leuciscidae). 更正：鲤形目：鯈鱼X和Y染色体广泛遗传分化的有限证据。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-08-20 DOI: 10.1093/jhered/esae038

引用次数: 0

Quantitative trait loci concentrate in specific regions of the Mexican cavefish genome and reveal key candidate genes for cave-associated evolution. 定量性状位点集中在墨西哥洞穴鱼基因组的特定区域，揭示了洞穴相关进化的关键候选基因。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-07-30 DOI: 10.1093/jhered/esae040

Jonathan Wiese, Emilie Richards, Johanna E Kowalko, Suzanne E McGaugh

{"title":"Quantitative trait loci concentrate in specific regions of the Mexican cavefish genome and reveal key candidate genes for cave-associated evolution.","authors":"Jonathan Wiese, Emilie Richards, Johanna E Kowalko, Suzanne E McGaugh","doi":"10.1093/jhered/esae040","DOIUrl":"10.1093/jhered/esae040","url":null,"abstract":"A major goal of modern biology is connecting phenotype with its underlying genetic basis. The Mexican cavefish (Astyanax mexicanus), a characin fish species comprised of a surface ecotype and a cave-derived ecotype, is well suited as a model to study the genetic mechanisms underlying adaptation to extreme environments. Here we map 206 previously published quantitative trait loci (QTL) for cave-derived traits in A. mexicanus to the newest version of the surface fish genome assembly, AstMex3. These analyses revealed that QTL cluster in the genome more than expected by chance, and this clustering is not explained by the distribution of genes in the genome. To investigate whether certain characteristics of the genome facilitate phenotypic evolution, we tested whether genomic characteristics associated with increased opportunities for mutation, such as highly mutagenic CpG sites, are reliable predictors of the sites of trait evolution but did not find any significant trends. Finally, we combined the QTL map with previously collected expression and selection data to identify 36 candidate genes that may underlie the repeated evolution of cave phenotypes, including rgrb, which is predicted to be involved in phototransduction. We found this gene has disrupted exons in all non-hybrid cave populations but intact reading frames in surface fish. Overall, our results suggest specific regions of the genome may play significant roles in driving adaptation to the cave environment in Astyanax mexicanus and demonstrate how this compiled dataset can facilitate our understanding of the genetic basis of repeated evolution in the Mexican cavefish.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141857224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal outbreeding is shaped during larval life-history in the splash pool copepod Tigriopus californicus. 溅池桡足类（Tigriopus californicus）在幼虫生活史中形成了最佳的外繁殖。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-07-26 DOI: 10.1093/jhered/esae039

Kevin C Olsen, Luis D Escareno Medina, Felipe S Barreto, Suzanne Edmands, Ronald S Burton

{"title":"Optimal outbreeding is shaped during larval life-history in the splash pool copepod Tigriopus californicus.","authors":"Kevin C Olsen, Luis D Escareno Medina, Felipe S Barreto, Suzanne Edmands, Ronald S Burton","doi":"10.1093/jhered/esae039","DOIUrl":"https://doi.org/10.1093/jhered/esae039","url":null,"abstract":"Inbreeding and outbreeding depression are dynamic forms of selection critical to mating system evolution and the efficacy of conservation biology. Most evidence on how the relative severity and timing of these forces are shaped is confined to self-fertilization, distant outcrossing, and intermediate 'optimal outcrossing' in hermaphrodites. We tested the notion that closed population demographics may reduce and delay the costs of inbreeding relative to distant outbreeding in an intertidal copepod with separate sexes and a biphasic larval / post-metamorphic life-history (Tigriopus californicus). At three lifecycle stages (fecundity, metamorphosis, and post-metamorphosis), we quantified the effects of inbreeding and outbreeding in crosses with varying degrees of recent common ancestry. Although inbreeding and outbreeding depression have distinct genetic mechanisms, both manifested the same stage-specific consequences for fitness. Inbreeding and outbreeding depression were not apparent for fecundity, post-metamorphic survival, sex ratio, or the ability to acquire mates, but inbreeding between full siblings and outbreeding between interpopulation hybrids reduced the fraction of offspring that completed metamorphosis by 32% and 47%, respectively. On average, the effects of inbreeding on metamorphic rate were weaker and nearly twice as variable among families than those of outbreeding, suggesting genetic load was less pervasive than the incompatibilities accrued between divergent populations. Overall, our results indicate the transition from larval to juvenile life stages is markedly susceptible to both inbreeding and outbreeding depression in T. californicus. We suggest stage-specific selection acting concurrently with the timing of metamorphosis may be an instrumental factor shaping reproductive optima in species with complex life-histories.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141762718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference genome for the Mojave poppy bee (Perdita meconis), a specialist pollinator of conservation concern. 莫哈韦罂粟蜂（Perdita meconis）的参考基因组，这是一种受保护的专业授粉动物。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-07-10 DOI: 10.1093/jhered/esad076

Rena M Schweizer, Colleen G Meidt, Ligia R Benavides, Joseph S Wilson, Terry L Griswold, Sheina B Sim, Scott M Geib, Michael G Branstetter

{"title":"Reference genome for the Mojave poppy bee (Perdita meconis), a specialist pollinator of conservation concern.","authors":"Rena M Schweizer, Colleen G Meidt, Ligia R Benavides, Joseph S Wilson, Terry L Griswold, Sheina B Sim, Scott M Geib, Michael G Branstetter","doi":"10.1093/jhered/esad076","DOIUrl":"10.1093/jhered/esad076","url":null,"abstract":"The Mojave poppy bee, Perdita meconis Griswold (Hymenoptera: Anthophila: Andrenidae), is a species of conservation concern that is restricted to the eastern Mojave Desert of North America. It is a specialist pollinator of two poppy genera, Arctomecon and Argemone (Papaveraceae), and is being considered for listing under the US Endangered Species Act along with one of its pollinator hosts, the Las Vegas bearpoppy (Arctomecon californica). Here, we present a near chromosome-level genome of the Mojave poppy bee to provide a genomic resource that will aid conservation efforts and future research. We isolated DNA from a single, small (<7 mm), male specimen collected using non-ideal preservation methods and then performed whole-genome sequencing using PacBio HiFi technology. After quality and contaminant filtering, the final draft genome assembly is 327 Mb, with an N50 length of 17.5 Mb. Annotated repetitive elements compose 37.3% of the genome, although a large proportion (24.87%) of those are unclassified repeats. Additionally, we annotated 18,245 protein-coding genes and 19,433 transcripts. This genome represents one of only a few genomes from the large bee family Andrenidae and one of only a few genomes for pollinator specialists. We highlight both the potential of this genome as a resource for future research, and how high-quality genomes generated from small, non-ideal (in terms of preservation) specimens could facilitate biodiversity genomics.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"470-479"},"PeriodicalIF":3.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11235129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An inside "beak": Molecular analysis of swab samples reveals the seabird diet of invasive Barn Owls in Hawai'i. 内部“喙”：对拭子样本的分子分析揭示了夏威夷入侵仓猫头鹰的海鸟饮食。

IF 3 2区生物学

Journal of Heredity Pub Date : 2024-07-10 DOI: 10.1093/jhered/esad068

Joanna W Elmore, Taylor M Wilcox, Alex E Dutcher, Yuki Reiss, Michael K Schwartz

{"title":"An inside \"beak\": Molecular analysis of swab samples reveals the seabird diet of invasive Barn Owls in Hawai'i.","authors":"Joanna W Elmore, Taylor M Wilcox, Alex E Dutcher, Yuki Reiss, Michael K Schwartz","doi":"10.1093/jhered/esad068","DOIUrl":"10.1093/jhered/esad068","url":null,"abstract":"Predation is an important species interaction to monitor when assessing an invasive species' impact on a particular ecosystem, but it can be difficult to observe and thus, fully understand. On Kaua'i island, invasive Barn Owls (Tyto alba) predate native seabirds, but difficult terrain in this region and the cryptic nature of owl predation make traditional monitoring of predation quite challenging. Using Barn Owls collected as part of removal efforts on Kaua'i and Lehua islands, we conducted DNA metabarcoding of owl digestive tracts to detect and determine seabird species they predate. We used a seabird-targeted 12s marker to sequence 112 swabs from 55 owls and detected 6 seabird taxa, including 2 ESA-listed seabirds-Hawaiian Petrel (Pterodroma sandwichensis) and Newell's Shearwater (Puffinus newelli), in 12 swabs from 11 owls (20% of sampled owls). Corresponding morphological assessment of owl stomach contents detected seabird species as prey items in only 2% (1/55) of sampled owls, highlighting the utility of molecular approaches for detecting diet items, especially degraded or visually absent items. Additionally, this approach has proven very useful in revealing cryptic trophic interactions in inaccessible seabird populations. For the most comprehensive analysis of diet, the use of both esophageal and cloacal swabs for metabarcoding is recommended. Supplementing metabarcoding with other methods that can provide complementary prey information, such as stable isotope analysis, would help to characterize trophic interactions more fully. The method described here has proven to be a reliable tool for investigating diet in invasive owls and may be used to investigate cryptic predation in living birds as a minimally invasive technique, as well.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"432-443"},"PeriodicalIF":3.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72016200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0