Cytogenomic analysis in Seriemas (Cariamidae): Insights into an Atypical Avian Karyotype.

IF 3 2区 生物学 Q2 EVOLUTIONARY BIOLOGY
Guilherme Mota Souza, Jhon Alex Dziechciarz Vidal, Ricardo Utsunomia, Geize Aparecida Deon, Edivaldo Herculano Correa de Oliveira, Raqueli Teresinha Franca, Fabio Porto-Foresti, Thomas Liehr, Fernando Henrique Santos de Souza, Rafael Kretschmer, Marcelo de Bello Cioffi
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引用次数: 0

Abstract

Contrasting with most bird species that present an ancestral-like karyotype (with 2n = 80), the only extant Cariamidae birds, the Red-legged (Cariama cristata) and Black-legged (Chunga burmeisteri) Seriemas, have high 2n and atypically large Z chromosomes. This study combined cytogenetic, bioinformatic, and genomic analyses to examine the distinctive characteristics of an unusual bird karyotype, with a focus on repetitive elements and sex chromosomes. Whole-genome alignments and chromosomal painting with a Z-chromosome-specific probe were also performed against the emu (a species with an ancestral-like karyotype). The satellitomes of C. cristata and C. burmeisteri were composed of only four and 6 long satDNAs, respectively. These satDNAs showed similarity with other repetitive sequences, mostly transposable elements, and were mapped in the pericentromeric regions of several chromosome pairs. CcrSat02-1104 mostly covered the Z and W sex chromosomes, besides being spread throughout additional chromosomes. Interstitial telomeric sites were not detected, even in the Z chromosome, and none of the 16 microsatellites tested showed positive signals on the C. cristata chromosomes. The genome alignments showed that the karyotype evolution that occurred in C. cristata may have involved significant chromosomal reshuffling, particularly fission. Notwithstanding certain internal inversions, the Z chromosome retained homology with that of the emu. However, repetitive sequences also accumulated on the Z chromosome, contributing to its enlargement relative to the pattern observed in ancestral avian groups.

Seriemas (Cariamidae) 的细胞基因组分析:对非典型禽类核型的见解。
与大多数具有祖先样核型(2n = 80)的鸟类不同,仅存的红腿鸟(Cariama cristata)和黑腿鸟(Chunga burmeisteri) seremas具有高2n和非典型大Z染色体。本研究结合细胞遗传学、生物信息学和基因组学分析来研究一种不寻常的鸟类核型的独特特征,重点关注重复元素和性染色体。对鸸鹋(一种具有祖先样核型的物种)也进行了全基因组比对和z染色体特异性探针的染色体绘制。柽柳和柽柳的卫星体分别由4个和6个长卫星dna组成。这些satdna显示出与其他重复序列的相似性,主要是转座元件,并被定位在几对染色体的中心点周围区域。CcrSat02-1104除了分布在其他染色体上外,主要覆盖Z和W性染色体。即使在Z染色体上,也未检测到间质端粒位点,16个微卫星在冠棘染色体上均未显示阳性信号。基因组比对结果表明,鸡冠花的核型进化可能涉及染色体重组,特别是裂变。尽管有某些内部反转,但Z染色体与鸸鹋的染色体保持同源性。然而,重复序列也在Z染色体上积累,导致其相对于在祖先鸟类群体中观察到的模式扩大。
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来源期刊
Journal of Heredity
Journal of Heredity 生物-遗传学
CiteScore
5.20
自引率
6.50%
发文量
63
审稿时长
6-12 weeks
期刊介绍: Over the last 100 years, the Journal of Heredity has established and maintained a tradition of scholarly excellence in the publication of genetics research. Virtually every major figure in the field has contributed to the journal. Established in 1903, Journal of Heredity covers organismal genetics across a wide range of disciplines and taxa. Articles include such rapidly advancing fields as conservation genetics of endangered species, population structure and phylogeography, molecular evolution and speciation, molecular genetics of disease resistance in plants and animals, genetic biodiversity and relevant computer programs.
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