Journal of Heredity最新文献

Phylogenetic relationships and species delimitation of bunchgrass lizards of the genus Sceloporus from Mexico with the description of a new species.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-04-03 DOI: 10.1093/jhered/esaf020

Tristan Charran, Jesús A Loc-Barragan, Rafael Alejandro Calzada-Arciniega, Liz A Alfaro-Juantorena, Raúl Cueva Del Castillo, Christopher Blair, Víctor H Jiménez-Arcos

{"title":"Phylogenetic relationships and species delimitation of bunchgrass lizards of the genus Sceloporus from Mexico with the description of a new species.","authors":"Tristan Charran, Jesús A Loc-Barragan, Rafael Alejandro Calzada-Arciniega, Liz A Alfaro-Juantorena, Raúl Cueva Del Castillo, Christopher Blair, Víctor H Jiménez-Arcos","doi":"10.1093/jhered/esaf020","DOIUrl":"https://doi.org/10.1093/jhered/esaf020","url":null,"abstract":"Molecular data continue to serve as an invaluable tool to assess species limits and patterns of cryptic diversity, particularly in areas harboring high levels of biodiversity. The Sceloporus scalaris group, known as bunchgrass lizards, contains 13 described and one undescribed species distributed throughout high elevation biotas of Mexico. However, recent studies suggest that diversity in the group may be substantially underestimated. We collect new sequence data from four independent loci to determine the evolutionary distinctiveness and phylogenetic relationships of an isolated population of bunchgrass lizard in the Sierra San Juan (SSJ) in the Mexican state of Nayarit. Concatenation and coalescent-based phylogenetic and species delimitation analyses suggest that this population represents a distinct evolutionary lineage allied to the S. scalaris group. We describe the new SSJ population as a new species to better account for its morphological and evolutionary distinctiveness. Divergence within the species occurred during the Neogene and Quaternary, most likely due to range shifts associated with mountain uplift and glacial-interglacial cycles. Our results provide additional evidence that supports the importance of the Mexican highlands as a biodiversity hotspot which maintains cryptic lineages awaiting discovery.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143774983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carotenoids, instead of pteridines, determine color of xanthophores and erythrophores in tilapia.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-25 DOI: 10.1093/jhered/esaf017

Guangyuan Liang, Jiawen Yao, Jingzhi Wu, Xiayue Liu, Zilong Wen, Hao Liu, Baoyue Lu, Chenxu Wang, Xingyong Liu, Deshou Wang

{"title":"Carotenoids, instead of pteridines, determine color of xanthophores and erythrophores in tilapia.","authors":"Guangyuan Liang, Jiawen Yao, Jingzhi Wu, Xiayue Liu, Zilong Wen, Hao Liu, Baoyue Lu, Chenxu Wang, Xingyong Liu, Deshou Wang","doi":"10.1093/jhered/esaf017","DOIUrl":"https://doi.org/10.1093/jhered/esaf017","url":null,"abstract":"There has been controversy over whether the chromogenic substances of xanthophores and erythrophores are pteridines or carotenoids in fish. In this study, we first extracted and quantified carotenoids and pteridines from erythrophores and xanthophores in tilapia, respectively. The results showed that the carotenoids and pteridines in erythrophores were significantly higher than those of xanthophores, and the carotenoids were significantly higher than the pteridines in both types of cells. Then, we established homozygous mutant lines of the key genes for pteridine synthesis (xdh) and carotenoid metabolism (plin6) in Nile tilapia. Compared with the wild type (WT), there were no significant changes in the body color of xdh-/-, while the pigmentation, fluorescence intensity and size of both xanthophores and erythrophores were significantly reduced in plin6-/-. The pteridines were significantly decreased in xdh-/-, but not in plin6-/-, while the carotenoids were significantly decreased in plin6-/-, but not in xdh-/-. To confirm these results, we further established homozygous mutant lines for another two key genes involved in pteridine synthesis (gch2) and carotenoid absorption (scarb1). Consistently, no significant body color changes were observed in gch2-/-, while no pigmented xanthophores and erythrophores were observed in scarb1-/-. The pteridines were significantly reduced in gch2-/-, but not in scarb1-/-, while almost no carotenoids were detected in scarb1-/-, but carotenoids remained unchanged in gch2-/- compared with WT. Taken together, these results, combined with existing findings in cyprinid fish, demonstrate that the color of xanthophores and erythrophores is determined by carotenoids rather than pteridines in fish.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First reference genomes for two mesophotic, reef-building coral species: Leptoseris cf. scabra and Montipora cf. grisea.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-22 DOI: 10.1093/jhered/esaf010

Veronica Z Radice, Johanna C Gijsbers, Silvia Vimercati, Daniel J Barshis

{"title":"First reference genomes for two mesophotic, reef-building coral species: Leptoseris cf. scabra and Montipora cf. grisea.","authors":"Veronica Z Radice, Johanna C Gijsbers, Silvia Vimercati, Daniel J Barshis","doi":"10.1093/jhered/esaf010","DOIUrl":"https://doi.org/10.1093/jhered/esaf010","url":null,"abstract":"Coral mortality is occurring worldwide at an alarming rate. Despite the immense and underestimated biodiversity of reef-building corals, very few genomes are available. Further, almost all genomic resources originate from shallow water corals even though photosynthetic, symbiotic corals occur at mesophotic depths deeper than 30 m and even >100 m. We present annotated, de novo genomes for two mesophotic, scleractinian (reef-building) corals Montipora cf. grisea and Leptoseris cf. scabra from American Sāmoa, the latter being the first genome for the widespread genus Leptoseris. We used PacBio continuous long reads and Omni-C data to assemble chromosome-level reference genomes. For Montipora cf. grisea, the final genome size was 1.3 Gb with a completeness level (BUSCO) of 99.9% and 97.2% against the eukaryotic and metazoan databases, respectively. The M. cf. grisea genome had a N50 of 50.2 Mb and the annotation predicted 41,981 genes. For Leptoseris cf. scabra, the final genome size was 794 Mb with a BUSCO of 99.2% and 96.1% against the eukaryotic and metazoan databases, respectively. The L. cf. scabra genome had a N50 of 45.2 Mb and 35,741 predicted genes. These genomes serve as critical references for the analysis of coral gene expression responses to climate change such as ocean warming (i.e., coral bleaching) and ocean acidification impacts. The genomes can be used to investigate the genetic diversity and adaptive divergence of shallow vs. mesophotic coral populations to understand reef resilience and guide conservation strategies.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first de novo genome assembly and annotation of a green-blooded skink (Prasinohaema aff. flavipes) from a historic museum sample. 首次从历史悠久的博物馆样本中对绿血石龙子（Prasinohaema aff.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-22 DOI: 10.1093/jhered/esaf014

Taylor S Probst, Daniel D Davis, Alison S Whiting, Paul B Frandsen

{"title":"The first de novo genome assembly and annotation of a green-blooded skink (Prasinohaema aff. flavipes) from a historic museum sample.","authors":"Taylor S Probst, Daniel D Davis, Alison S Whiting, Paul B Frandsen","doi":"10.1093/jhered/esaf014","DOIUrl":"https://doi.org/10.1093/jhered/esaf014","url":null,"abstract":"The scincid genus Prasinohaema represents the only group of amniotes to possess green blood, resulting from the accumulation of the bile pigment biliverdin. Recent phylogenetic analyses have shown Prasinohaema to be polyphyletic with 4 independent origins for green blood. It is unknown why this trait has evolved in multiple instances and how Prasinohaema species are able to tolerate such high concentrations of a cytotoxic bile pigment. Here, we report the first de novo genome assembly and annotation for a green blooded skink, Prasinohaema aff. flavipes, which was sequenced from an ethanol preserved specimen that was collected more than 20 years ago. Our assembly comprises 907 contigs spanning 1.52 Gbp with contig N50 of 3.2 Mbp. The genome is highly complete, with a BUSCO completeness score of 97.76%. Phylogenetic analysis confirmed that our specimen is sister to P. flavipes with high support; however, the two specimens exhibit deep genetic divergence, perhaps supporting their distinctiveness. Searches for the gene encoding serum albumin, a common blood transport protein known to bind biliverdin, suggested it is absent from our P. aff. flavipes genome. Yet, we found a tandem duplication of the serum albumin paralog alpha-fetoprotein, which may play a role in biliverdin retention. BUSTED analysis of alpha-fetoprotein sequences across reptiles revealed that both copies of alpha-fetoprotein from our Prasinohaema genome are under diversifying selection. We envision this new genome assembly as a valuable source in examining the key genes, regulatory mechanisms, and functional mutations involved in the elevated biliverdin levels.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into longhorn beetle (Cerambycidae) evolution from comparative analyses of the red-headed ash borer (Neoclytus acuminatus acuminatus) genome.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-22 DOI: 10.1093/jhered/esaf016

Terrence Sylvester, Richard Adams, Robert F Mitchell, Ann M Ray, Rongrong Shen, Na Ra Shin, Kasuni Daundasekara, Duane D McKenna

{"title":"Insights into longhorn beetle (Cerambycidae) evolution from comparative analyses of the red-headed ash borer (Neoclytus acuminatus acuminatus) genome.","authors":"Terrence Sylvester, Richard Adams, Robert F Mitchell, Ann M Ray, Rongrong Shen, Na Ra Shin, Kasuni Daundasekara, Duane D McKenna","doi":"10.1093/jhered/esaf016","DOIUrl":"https://doi.org/10.1093/jhered/esaf016","url":null,"abstract":"Neoclytus acuminatus acuminatus (NACU), the red-headed ash borer, is a wood-boring longhorn beetle (Cerambycidae: Cerambycinae) native to North America and introduced in Eurasia and South America. Its larvae develop in dying or recently dead hardwood trees, including ecologically and economically significant species of ash, hickory, and oak. We sequenced, assembled, and annotated the genome of a female NACU, and compared it to the publicly available genomes of other cerambycid species. The 508 Mb NACU genome assembly spanned 20 contigs (19 nuclear + 1 mitochondrial), with an N50 of 52.59 Mb and largest contig of 61.20 Mb. A moderately high fraction of the genome (62.63%) comprised repetitive sequences, with nearly all (99.4%) expected orthologous genes (BUSCOs) present and fully assembled. We identified two contigs as fragments of the NACU sex chromosome. Genome annotation identified 12,899 genes, including 109 putative horizontally transferred loci. Synteny analysis identified well-conserved blocks of collinearity between the NACU genome and other Cerambycidae. The genome contains a similar number of genes encoding putative plant cell wall degrading enzymes as other Cerambycidae. The NACU genome provides new insights into genome evolution in the family Cerambycidae, known for its rich diversity of xylophagous species, and provides a new viewpoint from which to study the evolution and genomic basis of traits such as wood-feeding and olfaction in beetles and other insects.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenomic analysis in Seriemas (Cariamidae): Insights into an Atypical Avian Karyotype. Seriemas (Cariamidae) 的细胞基因组分析：对非典型禽类核型的见解。

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-12 DOI: 10.1093/jhered/esaf012

Guilherme Mota Souza, Jhon Alex Dziechciarz Vidal, Ricardo Utsunomia, Geize Aparecida Deon, Edivaldo Herculano Correa de Oliveira, Raqueli Teresinha Franca, Fabio Porto-Foresti, Thomas Liehr, Fernando Henrique Santos de Souza, Rafael Kretschmer, Marcelo de Bello Cioffi

{"title":"Cytogenomic analysis in Seriemas (Cariamidae): Insights into an Atypical Avian Karyotype.","authors":"Guilherme Mota Souza, Jhon Alex Dziechciarz Vidal, Ricardo Utsunomia, Geize Aparecida Deon, Edivaldo Herculano Correa de Oliveira, Raqueli Teresinha Franca, Fabio Porto-Foresti, Thomas Liehr, Fernando Henrique Santos de Souza, Rafael Kretschmer, Marcelo de Bello Cioffi","doi":"10.1093/jhered/esaf012","DOIUrl":"https://doi.org/10.1093/jhered/esaf012","url":null,"abstract":"Contrasting with most bird species that present an ancestral-like karyotype (with 2n = 80), the only extant Cariamidae birds, the Red-legged (Cariama cristata) and Black-legged (Chunga burmeisteri) Seriemas, have high 2n and atypically large Z chromosomes. This study combined cytogenetic, bioinformatic, and genomic analyses to examine the distinctive characteristics of an unusual bird karyotype, with a focus on repetitive elements and sex chromosomes. Whole-genome alignments and chromosomal painting with a Z-chromosome-specific probe were also performed against the emu (a species with an ancestral-like karyotype). The satellitomes of C. cristata and C. burmeisteri were composed of only four and 6 long satDNAs, respectively. These satDNAs showed similarity with other repetitive sequences, mostly transposable elements, and were mapped in the pericentromeric regions of several chromosome pairs. CcrSat02-1104 mostly covered the Z and W sex chromosomes, besides being spread throughout additional chromosomes. Interstitial telomeric sites were not detected, even in the Z chromosome, and none of the 16 microsatellites tested showed positive signals on the C. cristata chromosomes. The genome alignments showed that the karyotype evolution that occurred in C. cristata may have involved significant chromosomal reshuffling, particularly fission. Notwithstanding certain internal inversions, the Z chromosome retained homology with that of the emu. However, repetitive sequences also accumulated on the Z chromosome, contributing to its enlargement relative to the pattern observed in ancestral avian groups.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive sampling reveals landscape genetic structure in the threatened ghost bat (Macroderma gigas) in an ore-rich region of Western Australia.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-11 DOI: 10.1093/jhered/esaf011

Linette Umbrello, Rujiporn Thavornkanlapachai, Shelley McArthur, Diana Prada, Chris Knuckey, Robyn Shaw, Peter Spencer, Kym Ottewell

{"title":"Non-invasive sampling reveals landscape genetic structure in the threatened ghost bat (Macroderma gigas) in an ore-rich region of Western Australia.","authors":"Linette Umbrello, Rujiporn Thavornkanlapachai, Shelley McArthur, Diana Prada, Chris Knuckey, Robyn Shaw, Peter Spencer, Kym Ottewell","doi":"10.1093/jhered/esaf011","DOIUrl":"https://doi.org/10.1093/jhered/esaf011","url":null,"abstract":"Bat species are expected to exhibit low genetic structuring due to their high mobility. Thus, habitat connectivity is important to maintain gene flow and genetic diversity to retain evolutionary potential. The ghost bat (Macroderma gigas) is a large carnivorous bat endemic to Australia. Listed as Vulnerable, the species has a disjunct distribution across northern Australia and is patchily distributed at local scales due to limited roost habitat availability and anthropogenic impacts. Here, we survey the genetic diversity and structure of M. gigas in the isolated, arid Pilbara bioregion in Western Australia, primarily using non-invasively collected faecal DNA samples obtained from roosts. Faecal and tissue samples, representing 399 individuals, were genotyped using an optimised autosomal marker panel, with a subset also being sequenced at the mitochondrial D-Loop region to investigate historical gene flow. Spatially-explicit Bayesian clustering analyses of autosomal markers revealed low genetic structure and high levels of gene flow amongst the two Pilbara subregions, with some further structuring evident within the Hamersley Ranges. Mitochondrial DNA sequencing showed strong geographic structuring of haplotypes between the subpopulations, with only a small number of shared haplotypes indicating low levels of maternal gene flow. Such patterns across the two marker types are consistent with maternal philopatry and male-mediated gene flow that has previously been described for this species. Conservation actions for the ghost bat in the Pilbara should therefore recognise maintenance of connectivity between roosts and subregions is important to maintain gene flow for this threatened species in the face of anthropogenic threats.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for the existence of the distinct Alia camel breed contributes to the conservation of dromedary camels in Europe.

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-05 DOI: 10.1093/jhered/esaf009

Marcel Smits

引用次数: 0

Absence of heterosis for hypoxia tolerance in F1 hybrids of Tigriopus californicus. Tigriopus californicus 的 F1 代杂交种在耐缺氧性方面缺乏异质性。

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-01 DOI: 10.1093/jhered/esae061

Aimee Deconinck, Olivia F Madalone, Christopher S Willett

{"title":"Absence of heterosis for hypoxia tolerance in F1 hybrids of Tigriopus californicus.","authors":"Aimee Deconinck, Olivia F Madalone, Christopher S Willett","doi":"10.1093/jhered/esae061","DOIUrl":"10.1093/jhered/esae061","url":null,"abstract":"Hybridization produces a range of outcomes from advantageous to disadvantageous, and a goal of genetic research is to understand the gene interactions that generate these outcomes. Interactions between cytoplasmic elements, such as mitochondria, and the nucleus may be particularly vulnerable to accruing disadvantageous combinations as a result of their different rates of evolution. Consequently, mitonuclear incompatibilities may play an important role in hybrid outcomes even if their negative impacts could be masked for some fitness measures by heterosis in first-generation (F1) hybrids. We used Tigriopus californicus, a model system for mitonuclear incompatibilities that is also known for exhibiting heterosis in the F1 generation and outbreeding depression in later generations, to test whether heterosis or outbreeding depression would occur when mitonuclear mismatch was paired with a stress that heavily impacts mitochondrial processes-specifically, hypoxia. We generated 284 parental and 436 F1 hybrids from four population crosses (720 total) and compared parental and F1 populations for hypoxia tolerance. We observed that, on average, F1 hybrids were less likely to survive a hypoxia stress test than parental populations, although we did not detect a statistically significant trend (P = 0.246 to 0.614). This suggests that hypoxia may be a particularly intense stressor for mitonuclear coordination and hybridization outcomes vary by trait.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"149-158"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal outbreeding is shaped during larval life history in the splash pool copepod Tigriopus californicus. 溅池桡足类（Tigriopus californicus）在幼虫生活史中形成了最佳的外繁殖。

IF 3 2区生物学

Journal of Heredity Pub Date : 2025-03-01 DOI: 10.1093/jhered/esae039

Kevin C Olsen, Luis D Escareno Medina, Felipe S Barreto, Suzanne Edmands, Ronald S Burton

{"title":"Optimal outbreeding is shaped during larval life history in the splash pool copepod Tigriopus californicus.","authors":"Kevin C Olsen, Luis D Escareno Medina, Felipe S Barreto, Suzanne Edmands, Ronald S Burton","doi":"10.1093/jhered/esae039","DOIUrl":"10.1093/jhered/esae039","url":null,"abstract":"Inbreeding and outbreeding depression are dynamic forms of selection critical to mating system evolution and the efficacy of conservation biology. Most evidence on how the relative severity and timing of these forces are shaped is confined to self-fertilization, distant outcrossing, and intermediate \"optimal outcrossing\" in hermaphrodites. We tested the notion that closed population demographics may reduce and delay the costs of inbreeding relative to distant outbreeding in an intertidal copepod with separate sexes and a biphasic larval/post-metamorphic life history (Tigriopus californicus). At three lifecycle stages (fecundity, metamorphosis, and post-metamorphosis), we quantified the effects of inbreeding and outbreeding in crosses with varying degrees of recent common ancestry. Although inbreeding and outbreeding depression have distinct genetic mechanisms, both manifested the same stage-specific consequences for fitness. Inbreeding and outbreeding depression were not apparent for fecundity, post-metamorphic survival, sex ratio, or the ability to acquire mates, but inbreeding between full siblings and outbreeding between interpopulation hybrids reduced the fraction of offspring that completed metamorphosis by 32% and 47%, respectively. On average, the effects of inbreeding on metamorphic rate were weaker and nearly twice as variable among families than those of outbreeding, suggesting genetic load was less pervasive than the incompatibilities accrued between divergent populations. Overall, our results indicate the transition from larval to juvenile life stages is markedly susceptible to both inbreeding and outbreeding depression in T. californicus. We suggest stage-specific selection acting concurrently with the timing of metamorphosis may be an instrumental factor in shaping reproductive optima in species with complex life histories.","PeriodicalId":54811,"journal":{"name":"Journal of Heredity","volume":" ","pages":"159-169"},"PeriodicalIF":3.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141762718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0