Journal of Toxicology and Environmental Health-Part A-Current Issues最新文献_第6页

Do oil droplets and chemical dispersants contribute to uptake of oil compounds and toxicity of crude oil dispersions in cold-water copepods? 油滴和化学分散剂是否有助于冷水桡足类对油化合物的吸收和原油分散体的毒性？

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-17 Epub Date: 2023-10-23 DOI: 10.1080/15287394.2023.2271003

Bjørn Henrik Hansen, Dag Altin, Trond Nordtug

{"title":"Do oil droplets and chemical dispersants contribute to uptake of oil compounds and toxicity of crude oil dispersions in cold-water copepods?","authors":"Bjørn Henrik Hansen, Dag Altin, Trond Nordtug","doi":"10.1080/15287394.2023.2271003","DOIUrl":"10.1080/15287394.2023.2271003","url":null,"abstract":"Accidental crude oil spills to the marine environment cause dispersion of oil into the water column through the actions of breaking waves, a process that can be facilitated using chemical dispersants. Oil dispersions contain dispersed micron-sized oil droplets and dissolved oil components, and the toxicity of oil dispersions has been assumed to be associated primarily with the latter. However, most hydrophobic, bioaccumulative and toxic crude oil components are retained within the droplets which may interact with marine filter-feeders. We here summarize the findings of 15 years of research using a unique methodology to generate controlled concentrations and droplet size distributions of dispersed crude oil to study effects on the filter-feeding cold-water copepod Calanus finmarchicus. We focus primarily on the contribution of chemical dispersants and micron-sized oil droplets to uptake and toxicity of oil compounds. Oil dispersion exposures cause PAH uptake and oil droplet accumulation on copepod body surfaces and inside their gastrointestinal tract, and exposures to high exposure (mg/L range) reduce feeding activity, causes reproductive impairments and mortality. These effects were slightly higher in the presence of chemical dispersants, possibly due to higher filtration of chemically dispersed droplets. For C. finmarchicus, dispersions containing oil droplets caused more severe toxic effects than filtered dispersions, thus, oil droplets contribute to the observed toxicity. The methodology for generating crude oil dispersion is a valuable tool to isolate impacts of crude oil microdroplets and can facilitate future research on oil dispersion toxicity and produce data to improve oil spill models.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"67-84"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of mine tailing exposure on the development of early life stages of the marine copepod Calanus finmarchicus. 尾矿暴露对海洋桡足类鳍脚类Calanus finmarchicus早期生命阶段发育的影响。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-17 Epub Date: 2023-10-30 DOI: 10.1080/15287394.2023.2274935

Julia Farkas, Linn H Svendheim, Ida B Øverjordet, Emlyn J Davies, Dag Altin, Trond Nordtug, Pål A Olsvik, Tjalling Jager, Bjørn Henrik Hansen

{"title":"Effects of mine tailing exposure on the development of early life stages of the marine copepod Calanus finmarchicus.","authors":"Julia Farkas, Linn H Svendheim, Ida B Øverjordet, Emlyn J Davies, Dag Altin, Trond Nordtug, Pål A Olsvik, Tjalling Jager, Bjørn Henrik Hansen","doi":"10.1080/15287394.2023.2274935","DOIUrl":"10.1080/15287394.2023.2274935","url":null,"abstract":"The demand for mineral resources is increasing mining activities worldwide. In Norway, marine tailing disposal (MTD) is practiced, introducing mineral particles into fjord ecosystems. We investigated the effects of two concentrations (high and low) of fine tailings from a CaCO3 processing plant on early life stages of the marine copepod Calanus finmarchicus. Results show that the exposure did not significantly impact hatching success or development in non- and early feeding life stages. However, feeding stage nauplii ingested tailings, which caused a significantly slower development in later nauplii stages in high exposure groups, with most individuals being two stages behind the control group. Further, high mortality occurred in late nauplii and early copepodite stages in low exposure groups, which could be caused by insufficient energy accumulation and depleted energy reserves during development. Individuals exposed to high exposure concentrations seemed to survive by arresting development and potentially by reduced activity, thereby conserving energy reserves. In nature, slower development could affect lipid storage buildup and reproduction.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"85-93"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spawning time in adult polar cod (Boreogadus saida) altered by crude oil exposure, independent of food availability. 原油暴露改变了成年极地鳕鱼的产卵时间，与食物供应无关。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-17 Epub Date: 2023-07-03 DOI: 10.1080/15287394.2023.2228535

Leah C Strople, Ireen Vieweg, Fekadu Yadetie, Derrick Kwame Odei, Anders Thorsen, Odd André Karlsen, Anders Goksøyr, Lisbet Sørensen, Antonio Sarno, Bjørn Henrik Hansen, Marianne Frantzen, Øyvind J Hansen, Velmurugu Puvanendran, Jasmine Nahrgang

{"title":"Spawning time in adult polar cod (Boreogadus saida) altered by crude oil exposure, independent of food availability.","authors":"Leah C Strople, Ireen Vieweg, Fekadu Yadetie, Derrick Kwame Odei, Anders Thorsen, Odd André Karlsen, Anders Goksøyr, Lisbet Sørensen, Antonio Sarno, Bjørn Henrik Hansen, Marianne Frantzen, Øyvind J Hansen, Velmurugu Puvanendran, Jasmine Nahrgang","doi":"10.1080/15287394.2023.2228535","DOIUrl":"10.1080/15287394.2023.2228535","url":null,"abstract":"Fish early life stages are well known for their sensitivity to crude oil exposure. However, the effect of crude oil exposure on adults and their gametes during their spawning period is not well studied. Polar cod, a key arctic fish, may be at risk for crude oil exposure during this potentially sensitive life stage. Additionally, this species experiences lower food availability during their spawning season, with unknown combined consequences. In the present study, wild-caught polar cod were exposed to decreasing levels of a water-soluble fraction (WSF) of crude oil or control conditions and fed either at a low or high feed ration to assess the combined effect of both stressors. Samples were taken during late gonadal development, during active spawning (spawning window), and in the post-spawning period. Histology analysis of gonads from fish sampled during the spawning window showed that oil-exposed polar cod were more likely to have spawned compared to controls. Oil-exposed females had 947 differentially regulated hepatic genes, and their eggs had a higher polycyclic aromatic hydrocarbon body burden compared to controls. Feed ration did not consistently affect polar cod's response to oil exposure for the endpoints measured, however, did alone result in decreases in some sperm motility parameters. These results suggest that polar cod's spawning period is a sensitive life event to crude oil exposure, while feed limitation may play a minor role for this supposedly capital breeder. The effects of adult exposure to crude oil on gamete quality and the next generation warrant further investigation.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"43-66"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9737642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic exposure to low-dose MC-LR induces ileal inflammation in mice through the PI3K/AKT/mTOR pathway. 慢性暴露于低剂量MC-LR通过PI3K/AKT/mTOR通路诱导小鼠回肠炎症。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-10 DOI: 10.1080/15287394.2024.2441294

Mingjie Yang, Shuilin Zheng, Wen Zeng, Chunhua Zhan, Yue Yang, Fei Yang

{"title":"Chronic exposure to low-dose MC-LR induces ileal inflammation in mice through the PI3K/AKT/mTOR pathway.","authors":"Mingjie Yang, Shuilin Zheng, Wen Zeng, Chunhua Zhan, Yue Yang, Fei Yang","doi":"10.1080/15287394.2024.2441294","DOIUrl":"https://doi.org/10.1080/15287394.2024.2441294","url":null,"abstract":"The global phenomenon of cyanobacterial bloom pollution is spreading globally due to climate change and eutrophication. It is well established that harmful cyanobacteria produce a wide range of toxins including microcystin-LR (MC-LR), a cyclic heptapeptide toxin known to damage various organs. The intestinal tract is the main site of MC-LR absorption and one of the targets susceptible to toxicity. Currently, studies on the enterotoxic effects of MC-LR predominantly focused on the colorectum, with limited investigations addressing the impact of microcystins on the small intestine. Therefore, the aim of our study was to examine the impact of chronic 9-month exposure of mice to low-dose 120 μg/L MC-LR in drinking water on ileal inflammation and potential mechanisms underlying these effects. Our findings showed that in mice chronically administered with low-dose MC-LR disorganized intestinal epithelial cells, lymphocytic infiltration and disturbed crypt arrangement were detected. The results of qPCR and Western blot demonstrated that, in comparison to control, the mRNA expression levels of pro-inflammatory factors IL-6, IL-17, IL-18, and IFN-γ were markedly elevated in the ileal tissue of mice treated with MC-LR, associated with significant increases in protein expression levels of p-PI3K, p-AKT, and p-mTOR. Taken together, evidence indicates that MC-LR induces ileal inflammation and histopathological damage involved activation of the PI3K/AKT/mTOR signaling pathway.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-9"},"PeriodicalIF":2.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing coil temperature of a third-generation e-cigarette device modulates C57BL/6 mouse lung immune cell composition and cytokine milieu independently of aerosol dose. 提高第三代电子烟装置的线圈温度可调节 C57BL/6 小鼠肺部免疫细胞的组成和细胞因子环境，而与气溶胶剂量无关。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-02 Epub Date: 2024-11-04 DOI: 10.1080/15287394.2024.2412998

Morgan E Poindexter, Yichen Li, Amy K Madl, Tran B Nguyen, Kent E Pinkerton

{"title":"Increasing coil temperature of a third-generation e-cigarette device modulates C57BL/6 mouse lung immune cell composition and cytokine milieu independently of aerosol dose.","authors":"Morgan E Poindexter, Yichen Li, Amy K Madl, Tran B Nguyen, Kent E Pinkerton","doi":"10.1080/15287394.2024.2412998","DOIUrl":"10.1080/15287394.2024.2412998","url":null,"abstract":"Higher coil temperature in e-cigarette devices increases the formation of aerosols and toxicants, such as carbonyls. At present, the health implications of vaping at higher temperatures, including exacerbation of pulmonary inflammation, are largely unknown when aerosol dose is considered. To isolate the pulmonary effects of coil temperature, C57BL/6 mice were exposed to e-cigarette aerosols generated at lower (190°C) or higher (250°C) temperature for 3 days, while maintaining a similar chamber aerosol concentration. Increasing coil temperature did not markedly alter aerosol mass-normalized emissions of select carbonyls formed from thermal degradation pathways including formaldehyde, acetaldehyde, propionaldehyde, and acetone under the tested environment. Total bronchoalveolar cells, primarily macrophages, were significantly decreased in mice exposed to aerosols generated with higher coil temperatures compared to lower temperature exposures. The gene expression of IFNβ, IL-1β, TNFα, and IL-10 in mouse lung tissue was significantly reduced following e-cigarette exposure under both conditions, compared to filtered air exposure. Higher temperature exposures further exacerbated downregulation of IFNβ and IL-1β. Data suggest that higher temperature vaping might modulate acute pulmonary immune responses, potentially inducing immune suppression, even when normalized for aerosol dose exposure. Coil temperature thus appears to be an important parameter that needs to be regulated to ensure harm reduction for e-cigarette users.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"20-33"},"PeriodicalIF":2.3,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between long-term exposure to fine particulate air pollution and risk of death attributed to esophageal cancer in Taiwan. 台湾长期暴露于微粒空气污染与食道癌死亡风险之间的关系。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-02 Epub Date: 2024-10-16 DOI: 10.1080/15287394.2024.2415318

Chun-Yuh Yang

{"title":"Association between long-term exposure to fine particulate air pollution and risk of death attributed to esophageal cancer in Taiwan.","authors":"Chun-Yuh Yang","doi":"10.1080/15287394.2024.2415318","DOIUrl":"10.1080/15287394.2024.2415318","url":null,"abstract":"The International Agency for Research on Cancer (IARC) classifies exposure to fine particulate matter (PM2.5) air pollution as carcinogenic to humans (Group I), most frequently associated with lung cancer. Airborne air pollutants may be associated with other sites of cancer, although few studies have examined this avenue of research. Esophageal cancer mortality rates vary substantially across townships in Taiwan, a fact that suggests environment influence. Therefore, the aim of this study was to investigate the association between long-term exposure to ambient PM2.5 and deaths attributed to esophageal cancer in 66 municipal areas across Taiwan. To conduct this study, annual PM2.5 levels were determined taking into account age-standardized esophageal cancer mortality rates in male and female residents of these municipalities from 2012 to 2021. The annual PM2.5 levels of each municipality were divided into tertiles and computed adjusted risk ratio (RR) using weighted-multiple regression analyses controlling for municipal lung cancer deaths, urbanization level, and physician density. Men residing in those areas with intermediate PM2.5 tertile levels (18.96-25.19 ug/m3) were found to have an adjusted RR of 1.22 (1.15-1.30) and those of residing areas with the highest tertiles levels (25.20-29.48 ug/m3) exhibited an RR of 1.11 (1.051.18). However, in women in the same municipalities, a significant inverse association was found between PM2.5 levels and mortality attributed to esophageal cancer, 0.82 (95% CI = 0.65-1.04) and 0.61 (95% CI = 0.47-0.79), respectively. These findings suggest that long-term exposure to PM2.5 increases the risk of developing esophageal cancer in men in Taiwan.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"34-42"},"PeriodicalIF":2.3,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using the National Land Cover Database as an indicator of shrub-steppe habitat: comparing two large United States federal lands with surrounding regions. 使用国家土地覆盖数据库作为灌木-干草原栖息地的指标：将两块大型美国联邦土地与周边地区进行比较。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-02 Epub Date: 2024-10-13 DOI: 10.1080/15287394.2024.2412659

Joanna Burger, Michael Gochfeld, Kevin G Brown, Monica Cortes, Kelly Ng, David Kosson

{"title":"Using the National Land Cover Database as an indicator of shrub-steppe habitat: comparing two large United States federal lands with surrounding regions.","authors":"Joanna Burger, Michael Gochfeld, Kevin G Brown, Monica Cortes, Kelly Ng, David Kosson","doi":"10.1080/15287394.2024.2412659","DOIUrl":"10.1080/15287394.2024.2412659","url":null,"abstract":"There is a need to assess whether ecological resources are being protected on large, federal lands. The aim of this study was to present a methodology which consistently and transparently determines whether two large Department of Energy (U.S. DOE) facilities have protected valuable ecological lands on their sites compared to the surrounding region. The National Land Cover Database (2019) was used to examine the % shrub-scrub (shrub-steppe) and other habitats on the DOE's Hanford Site (HS, Washington) and on the Idaho National Laboratory (INL), compared to a 10-km and 30-km diameter band of land surrounding each site. On both sites, over 95% is in shrub-scrub or grassland, compared to the surrounding region. Approximately 70% of 10 km and 30-km bands around INL, and less than 50% of land surrounding HS is located in these two habitat types. INL has preserved a significantly higher % shrub/scrub habitat than HS, but INL allows grazing on 60% of its land. HS has preserved a significantly higher % grassland than INL but no grazing on site is present. The methodology presented may be used to compare key ecological habitat types such as grasslands, forest, and desert among sites in different parts of the country. This methodology enables managers, resource trustees, and the public to (1) make remediation decisions that protect resources, (2) assess whether landowners and managers have adequately characterized and protected environmental resources on their sites, and (3) whether landowners and managers have protected the integrity of that land as well as its climax vegetation.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-19"},"PeriodicalIF":2.3,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple fluorometric test method for assessing skin sensitization potential of chemicals by using N-acetyl-L-cysteine methyl ester in chemico. 用n -乙酰- l-半胱氨酸甲酯评价化学品皮肤致敏电位的简单荧光试验方法。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-01 Epub Date: 2025-03-04 DOI: 10.1080/15287394.2025.2472224

Rahul Upadhyay Nepal, Tae Cheon Jeong

{"title":"A simple fluorometric test method for assessing skin sensitization potential of chemicals by using N-acetyl-L-cysteine methyl ester in chemico.","authors":"Rahul Upadhyay Nepal, Tae Cheon Jeong","doi":"10.1080/15287394.2025.2472224","DOIUrl":"10.1080/15287394.2025.2472224","url":null,"abstract":"The covalent binding of sensitizer to skin proteins is referred to as key event 1 of the adverse outcome pathway in skin sensitization. Recently, N-acetyl-L-cysteine methyl ester (NACME) was demonstrated to react selectively with skin sensitizers in vitro, such that NACME might be applied as an electron donor in developing a spectrophotometric test for determining skin sensitization potential of chemicals. To avoid possible color interference by certain test chemicals, a fluorometric test method was developed using monobromobimane (mBBr), a thiol-reactive fluorescent probe. Similar to previous methods utilizing the reaction of NACME with sensitizers, unreacted NACME occurred which was then measured fluorometrically using mBBr, rather than 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). Following the optimization of test conditions, the same 64 test chemicals used in the previous study were tested to determine the predictive capacity of the current method. Results showed a predictive capacity of 81.1% sensitivity, 81.5% specificity, and 81.3% accuracy with a cutoff NACME depletion of 11.3%. Although these values were relatively lower than the previous test using DTNB, the results were still comparable to OECD-approved test methods and that color interference issues might be ruled out. Data demonstrated that NACME might be viewed as a candidate for identifying reactive skin sensitizers. Further, this method might be considered as a complementary or supportive method to the former DTNB assay as a screening tool for assessing the tendency of a chemical to initiate skin sensitization in case of test chemicals showing color interference.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"589-603"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of polyphenols from Potentilla anserina L. against in rat myocardial ischemia-reperfusion on injury by Wnt/β-catenin pathway. 蕨麻多酚对Wnt/β-连环蛋白通路大鼠心肌缺血再灌注损伤的保护作用。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-01 Epub Date: 2025-03-20 DOI: 10.1080/15287394.2025.2470845

Jing Li, Meng-Yu Liu, Jian-Guo Li, He Zhang, Chun-Jie Zhang, Wei-Yi Ao, Ming Zhang, Xia-Guang Duan, Zhi-Ying Zhao

{"title":"Protective effect of polyphenols from Potentilla anserina L. against in rat myocardial ischemia-reperfusion on injury by Wnt/β-catenin pathway.","authors":"Jing Li, Meng-Yu Liu, Jian-Guo Li, He Zhang, Chun-Jie Zhang, Wei-Yi Ao, Ming Zhang, Xia-Guang Duan, Zhi-Ying Zhao","doi":"10.1080/15287394.2025.2470845","DOIUrl":"10.1080/15287394.2025.2470845","url":null,"abstract":"Myocardial ischemia reperfusion injury (MIRI) remains a major clinical challenge. Polyphenols derived from Potentilla anserina L (PPA) were found to possess anti-hypoxic-ischemic in vitro. The aim of this study was to examine (1) whether this plant exerts a protective effect and (2) whether the underlying mechanisms involving inflammatory responses and the Wnt/β-catenin signaling pathway were involved in rats. Rats were randomly divided as follows (1): Sham operation (2); ischemia-reperfusion (MIRI) (3); polyphenols (PPA) (4); Wnt inhibitor group (XAV939). In cardiomyocytes (1), HE staining was used to examine morphology (2), enzyme-linked immunosorbent assay to determine interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels and (3) Western blot to measure protein expression of Wnt and β-catenin. HE staining showed in MIRI edema of cardiomyocytes, localized inflammatory cell infiltration, unclear outline of cells and cell fragmentation, and some cardiomyocytes were seen to have disordered arrangement of tissues. PPA markedly lowered the morphological alterations in MIRI. The levels of levels of IL-1β and TNF-α in MIRI were significantly elevated; however, PPA reduced these cytokine concentrations compared to MIRI. Western blot analysis demonstrated that MIRI increased the relative protein expression levels of Wnt and β-catenin. Treatment of MIRI with PPA resulted in a significant decrease in protein expression levels of Wnt and β-catenin. The involvement of the Wnt/β-catenin pathway in MIRI was further affirmed with the use Wnt inhibitor group (XAV939) that also diminished protein expression levels of Wnt and β-catenin.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"567-575"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term effects of acute hazardous noise on auditory and non-auditory organs. 急性有害噪声对听觉和非听觉器官的长期影响。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-01-01 Epub Date: 2025-02-22 DOI: 10.1080/15287394.2025.2467177

Li Yang, Richard G Posner, Matthew C Salanga, Tinna Traustadóttir, O'neil W Guthrie

{"title":"Long-term effects of acute hazardous noise on auditory and non-auditory organs.","authors":"Li Yang, Richard G Posner, Matthew C Salanga, Tinna Traustadóttir, O'neil W Guthrie","doi":"10.1080/15287394.2025.2467177","DOIUrl":"10.1080/15287394.2025.2467177","url":null,"abstract":"Hazardous noise is a pervasive environmental pollutant with significant adverse health impacts on auditory and non-auditory organs. It is noteworthy that even acute noise exposure might pose immediate detrimental effects to various organs. However, the long-term effects of acute noise exposure remain largely unknown. This study aimed to explore this gap by randomizing 12 Long-Evans rats into acute noise and control groups. The acute noise regimen was a single three-hr wideband noise (12.5 hz-20 kHz) at 105 dB SPLpeak. Four weeks following exposure cessation, animals from both groups were sacrificed. Genomic DNA and RNA were extracted from the cochlea, brain, heart, and liver. Long-target polymerase assays and real-time quantitative polymerase chain reactions were performed to assess DNA integrity and p53-targeted gene expression, respectively, with results being compared between the two groups. Data demonstrated that noise-induced changes in DNA integrity depended upon organ type, with significant interaction effects between treatment conditions (noise or control) and organ type for nuclear and mitochondrial DNA integrity. In addition, there were significant changes in p53-targeted gene expression between noise-exposed and control in all tested organs. In conclusion, the long-term impact of acute hazardous noise exposure on DNA integrity was complex, highlighting organ-specificity in response to noise. However, such noise significantly altered p53-targeted genes systemically, indicating ongoing cellular stress. Overall, these results suggest that acute exposure to hazardous noise may have potential long-term adverse consequences. Immediate care following exposure might mitigate possible impacts on long-term health.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"519-535"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0