Journal of Toxicology and Environmental Health-Part A-Current Issues最新文献

{"title":"The analgesic effect of total saponins of Tribulus terrestris on neuropathic pain was studied based on TLR4/NF-κB pathway.","authors":"Wu-Xia Guo, He Zhang, Zhan-Qiang Wang, Jun Mao, Zhi-Ying Zhao, Ming Zhang","doi":"10.1080/15287394.2025.2470843","DOIUrl":"10.1080/15287394.2025.2470843","url":null,"abstract":"<p><p>Neuropathic pain (NP) consists of a range of unpleasant sensations attributed to a lesion or a disease of the somatosensory nervous system. It is important to note that the sensations initiated by NP are debilitating and adversely affect quality of life; however, the underlying mechanisms involved in the occurrence and development of this type of pain remain to be determined. Previously data demonstrated that inhibition of TLR4/NF-κB signaling pathway diminished the adverse consequences attributed to NP. Thus, the purpose of this study was to examine whether saponins derived from <i>Tribulus terrestris</i> might exert an analgesic effect on NP in rats using a chronic constriction injury (CCI) involving sciatic nerve. Male rats were randomly divided into Sham, CCI, low 100 mg/kg <i>T. terrestris</i>, high 200 mg/kg <i>T. terrestris</i>, and compared to pregabalin 10 mg/kg, the recognized first line of defense in NP. Three days after surgery, rats were treated with sham control (water) or drugs. The paw mechanical withdrawal threshold (PMWT) and thermal latency (TL) of rats were measured 1 day before operation and 3, 7, and 10 days after. Nissl staining was used to observe the morphology and Western Blot to detect protein expression of NF-κB and TLR4 on the 10th day after operation. ELISA was employed to detect levels of IL-1β, IL-6, and TNF-ɑ in spinal cord. CCI significantly decreased PMWT and TL. In CCI neurons and glial cells in the spinal dorsal horn were deeply stained with swelling and atrophy observed. The protein expression levels of NF-κB and TLR4 in the spinal dorsal horn of the injured side were significantly increased accompanied by elevated levels of inflammatory mediators IL-1β, IL-6, and TNF-ɑ. Treatment with low 100 mg/kg or high 200 mg/kg <i>T. terrestris</i> or pregabalin reduced effects of CCI on PMW, TL, histopathological changes as well as levels of inflammatory cytokines IL-1β and IL-6 associated with inhibition of TLR4/NF-κB pathway. Data suggest that <i>T. terrest</i> exerts an analgesic effect on peripheral NP which involves TLR4/NF-κB pathway inhibition.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"557-566"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to epoxiconazole induces hyperlocomotion in adult zebrafish.","authors":"Carlos G Reis, Rafael Chitolina, Leonardo M Bastos, Stefani M Portela, Thailana Stahlhofer-Buss, Angelo Piato","doi":"10.1080/15287394.2025.2519847","DOIUrl":"10.1080/15287394.2025.2519847","url":null,"abstract":"<p><p>Global agricultural production is sustained by an elevated use of pesticides. Their application results in environmental contamination, which is demonstrated by widespread detections of these chemicals in water. This presence poses a risk to non-target organisms and ecological balance. Epoxiconazole (EPX) is the active compound of widely employed triazole fungicides, frequently reported as water contaminants. However, the behavioral effects of EPX exposure in non-target organisms, such as fish, remain unexplored. Therefore, we aimed to investigate the effects of EPX (24, 144, and 240 μg/L) exposure on behavioral and neurochemical outcomes in zebrafish, at concentrations based on environmental detection. In the novel tank test, EPX exposure increased distance traveled, crossings, entries in the top area, and mean speed, indicating hyperlocomotion. No significant effects were observed in the social preference test and in the neurochemical analyses. These findings suggest that EPX exposure alters locomotor activity in zebrafish, highlighting the need for further research on its potential ecological impacts.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"967-980"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144303652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexmedetomidine pretreatment alleviates brain injury in middle cerebral artery occlusion (MCAO) model rats by activating PI3K/AKT/NF-κB signaling pathway.","authors":"Wei Gao, Xue Lv, Hao Li, Xu-Sheng Yan, Dong-Sheng Huo, Zhan-Jun Yang, Zhi-Guo Zhang, Jian-Xin Jia","doi":"10.1080/15287394.2025.2469088","DOIUrl":"10.1080/15287394.2025.2469088","url":null,"abstract":"<p><p>Cerebral ischemia-reperfusion injury (CIRI) is a prevalent clinical complication associated with reperfusion following ischemic stroke resulting in neuronal damage and cognitive impairment. Dexmedetomidine (DEX), a highly selective α2-adrenoceptor agonist with sedative, and analgesic properties, is frequently utilized as a sedative anesthetic in clinical surgeries, and believed to play a crucial role in the prognosis of patients suffering from CIRI. However, the mechanism underlying DEX in CIRI remains to be determined. This study aimed to investigate the neuroprotective effects of Dex in rats suffering from CIRI. In the treatment group, DEX (50 µg/kg) was administered intraperitoneally 30 min prior to surgery. Middle cerebral artery occlusion (MCAO) used as a model of CIRI occurred with cerebral artery occlusion for 2 h was followed by reperfusion with blood for 24, 72, 120 or 168 h. Neurological function as assessed by the Longa neurological function score test demonstrated significantly reduced neurological scores and increased % infarct size in MCAO group which was blocked by DEX suggesting that DEX might be effective in treating ischemic stroke. In the MCAO animals, 2,3,5-triphenyltetrazolium chloride (TTC) showed large marked areas of cerebral infarction which were diminished in size by DEX. Using Western blot analysis, results showed that in MCAO rats protein expression levels of TNF-α and IL-6 were increased accompanied by reduced protein expression levels of PI3K/AKT signaling pathway. DEX pretreatment reversed the effects of MCAO as evidenced by decrease in protein expression levels of TNF-α and IL-6 associated with elevated protein expression levels of PI3K/AKT/NF-κB signaling pathway. Data demonstrated that DEX pretreatment improved the neuromotor performance and cognitive functions in animals suffering from consequences of MCAO by diminishing inflammation and activation of the PI3K/AKT/NF-κB signaling pathway.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"546-556"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acteoside inhibits hepatic lipid accumulation and oxidative stress in type 2 diabetic mice via the Nrf2/HO-1 Pathway.","authors":"Dan-Dan Hao, Lei Zhang, Si-Lin Liu, Zhi-Juan Sun, Ri-Gu-Leng Si, Feng-Ning Zhang, Li-Xin Yang, Xiao-Li Jia, Tian-Zhu Li, Lin Ai, Chun-Ying Bai, Kai Gu, Cong-Ying Zhang","doi":"10.1080/15287394.2025.2473558","DOIUrl":"10.1080/15287394.2025.2473558","url":null,"abstract":"<p><p>Acteoside, a naturally occurring compound found in various plants, was found to exert antihyperlipidemic effects; however, the underlying mechanisms in nonalcoholic fatty liver disease associated with type 2 diabetes remain to be elucidated. This study aimed to examine (1) acteoside initiated hepatoprotection in diabetic mice and (2) whether the beneficial actions involved activation of Nrf2/HO-1 signaling pathway. Male mice given a high fat diet were injected with streptozotocin (STZ 50 mg/kg) to initiate type 2 diabetes mellitus (T2DM). Animals were randomly assigned to four groups (10 animals per group): (1) control, (2) diabetes, (3) acteoside (70 mg/kg) and (4) metformin (250 mg/kg). In diabetic mice, a significant increase in plasma levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) was accompanied by a fall in high-density lipoprotein (HDL). In diabetes, malondialdehyde (MDA) content was elevated in serum, accompanied by a decrease in superoxide dismutase (SOD) activity. Treatment with acteoside was found to significantly reduce plasma levels TC, TG, LDL, and VLDL accompanied by elevation in HDL, lowered MDA content accompanied by a rise in SOD activity. The metabolic alterations induced by metformin, the drug of choice in T2DM were similar to those noted for acteoside. Results showed that beneficial effects of acteoside involved activation of the Nrf2/HO-1 pathway. The potent antioxidant properties and lipid-lowering effects attributed to acteoside in T2DM may be considered as a promising therapeutic candidate for diabetic liver disease.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"616-625"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of essential oil from <i>Zingiber officinale</i> Roscoe against <i>Xanthomonas</i> strains: chemical composition and docking study of α-zingiberene.","authors":"Gleiciane C S Pereira, Cassia C Fernandes, Jardel Lopes Pereira, Daniela S Gonçalves, Gabriel G Calefi, Carlos H G Martins, Yan R Robles, Antônio E M Crotti, Erdi Can Aytar, Mayker L D Miranda","doi":"10.1080/15287394.2025.2514533","DOIUrl":"10.1080/15287394.2025.2514533","url":null,"abstract":"<p><p>Ginger rhizomes (<i>Zingiber officinale</i> Roscoe) have been used for treating several diseases including headaches, colds, nausea, and emesis. Considering the agrochemical potential of essential oil from ginger (EO-ZO), this study aimed to chemically characterize and assess its <i>in vitro</i> anti-<i>Xanthomonas</i> spp. activities. <i>Xanthomonas</i> species are plant pathogens capable of inducing certain diseases, such as canker, necrosis, and gall. For the first time, data demonstrated that EO-ZO exhibited high activity against <i>Xanthomonas citri, X. euvesicatoria, X. vasicola</i> and <i>X. campestris</i> since its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranged between 1.25 and 6.5 µg/ml. GC-MS and GC-FID revealed that α-zingiberene (50%), neral (15%), geranial (11%), and 1,8-cineole (9%) are the major constituents. A structure-based virtual screening approach was applied to α-zingiberene to predict antibacterial activity <i>in silico</i> and confirm its activity. In summary, EO-ZO appears to be a promising antimicrobial agent that may be used in sustainable and environmentally friendly agriculture.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"893-905"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aluminum and UV-C light on seed germination and initial growth of white oats.","authors":"Raissa Tainá Puntel, Raquel Stefanello, Wagner Jesus da Silva Garcia, Lucio Strazzabosco Dorneles","doi":"10.1080/15287394.2024.2405720","DOIUrl":"10.1080/15287394.2024.2405720","url":null,"abstract":"<p><p>Aluminum (Al) may be beneficial to crops, but in excess becomes detrimental to the germination and initial development of seedlings. The main determining indicators are the type of crop and exposure duration. The aim of this study was to examine the influence of Al and of UV-C light on the germination and initial growth of white oats. Seeds were sown on germitest paper in a solution of 100, 200, 300, 400, or 500 mg/L of aluminum chloride and kept in a germination chamber at 20°C for a 12-hr photoperiod. Germination and seedling growth parameters were determined after 5 and 10 days. The seeds were also exposed to two doses of UV-C (0.85 and 3.42 kJ m^-2) under aluminum chloride stress (200 mg/L). Data demonstrated that treatment with aluminum chloride significantly decrease in germination at 200 mg/L and total seedling length at 100 mg/L. Exposure of seeds to UV-C light under excess Al (200 mg/L) did not show a significant effect on germination and growth compared to control (non-irradiated). Results indicated that exposure to high concentration of Al in the medium adversely altered germination and initial growth of white oat seedlings. Although UV-C light alone was not detrimental to the germination process, treatment with UV-C light also failed to mitigate the toxic effects of Al.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"989-998"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142301233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing metal concentrations in hair and nails during longitudinal follow-up of apprentice welders.","authors":"Jairo Buitrago-Cortes, Philippe Sarazin, Denis Dieme, Naïma El Majidi, Michèle Bouchard","doi":"10.1080/15287394.2024.2410283","DOIUrl":"10.1080/15287394.2024.2410283","url":null,"abstract":"<p><p>The aim of this study was to determine factors influencing observed increased metal biomarkers of exposure levels in a group of 116 Quebec apprentice welders during a longitudinal follow-up of exposure. Analysis of 14 metals was carried out in hair, fingernail, and toenail samples taken from participants over the course of their welding curriculum at 6 different times. Personal and socio-demographic characteristics, lifestyle habits, and other potential confounding factors were documented by questionnaire. Multivariate linear mixed-effect models were used to assess main predictors of metal concentrations in each biological matrix including increasing time of exposure throughout the curriculum (defined as the repeated measure \"time\" variable\"). Significant associations between repeated measure \"time\" variable and metal levels in hair, fingernails, and toenails were found for chromium, iron, manganese and nickel. Significant associations with \"time\" were also noted for arsenic levels in hair and fingernails, and for barium, cobalt and vanadium levels in fingernails and toenails. The repeated measure \"time\" variable, hence increasing time of exposure throughout the curriculum, was the predominant predictor of elevated biological metal levels. Reduced spaces and simultaneous activities such as oxyfuel-cutting and welding in the same welding room were suspected to contribute to higher metal levels. Age, ethnicity, and annual household income exerted an effect on metal levels and considered as confounders in the models. Variations observed in metal levels between hair and nails of apprentice welders also emphasized the relevance and importance of performing multi-matrix and multi-element biomonitoring to assess temporal variations in biological metal concentrations during welding curriculum.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1030-1049"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of anthranilic diamide insecticides on metamorphosis in the common toad <i>Rhinella arenarum</i> (Hensel, 1867) at concentrations found in aquatic environments.","authors":"Shirley Vivian Daniela Fonseca Peña, Julie Céline Brodeur","doi":"10.1080/15287394.2024.2407479","DOIUrl":"10.1080/15287394.2024.2407479","url":null,"abstract":"<p><p>Anthranilic diamides (AD) are a modern class of insecticides used as alternatives to pyrethroids and neonicotinoids, particularly against lepidopteran pests. Despite their widespread use and presence in surface waters, little is known regarding their effects on amphibians. The aim of this study was to examine the effects of environmentally-relevant concentrations of AD insecticides chlorantraniliprole (CHLO) and cyantraniliprole (CYAN) on metamorphosis of the toad <i>Rhinella arenarum</i>. Tadpoles were exposed to CHLO or CYAN at concentrations ranging from 5 and 5000 µg/L from stage 27 until metamorphosis completion. Both insecticides produced a non-monotonic acceleration of the time required for individuals to progress through development and a decrease in the proportion of individuals completing metamorphosis, although a delay in metamorphosis was also observed at 5 µg/L of CHLO. Snout-vent length and body weight of metamorphosed toads were not markedly affected by either insecticide. CHLO was more toxic than CYAN, with a lowest observed effect concentration (LOEC) for CHLO on time to metamorphosis defined as 5 µg/L compared to 5000 µg/L for CYAN. The LOEC for reduced metamorphic success defined as 50 µg/L for CHLO compared to 500 µg/L for CYAN. As most effects occurred after stage 39, when metamorphosis depends upon thyroid hormones, it is conceivable that that AD insecticides act as endocrine disruptors. These findings suggest that contamination of surface waters with CHLO and CYAN may disrupt amphibian development in the wild and warrant further research to investigate the possibility of endocrine-disruption by ADs.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1015-1029"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selected micro- and macro-element associations with oxidative status markers in common carp (<i>Cyprinus carpio</i>) blood serum and ejaculate: a correlation study.","authors":"Marek Helczman, Marian Tomka, Julius Arvay, Eva Tvrda, Jaroslav Andreji, Martin Fik, Marek Snirc, Tomas Jambor, Peter Massanyi, Anton Kovacik","doi":"10.1080/15287394.2024.2406429","DOIUrl":"10.1080/15287394.2024.2406429","url":null,"abstract":"<p><p>The aim of this study was to (1) determine complex interactions between macro- and micro-elements present in blood serum and ejaculate of common carp (<i>Cyprinus carpio</i>), and (2) examine the association between alterations in these macro- and micro-elements with markers of oxidative stress. Blood and ejaculate from 10 male carp were collected in the summer period on the experimental pond in Kolíňany (West Slovak Lowland). Reactive oxygen species (ROS), total antioxidant capacity (TAC), protein carbonyls (PC), and malondialdehyde (MDA) levels were measured in blood serum and ejaculate using spectrophotometric methods. The amounts of elements (Ag, Al, Ba, Co, Li, Mo, Ca, K, Na, and Mg) in all samples were quantified using inductively coupled plasma optical emission spectrophotometry. Data demonstrated significant differences in elemental concentrations between blood and ejaculate, specifically significantly higher ejaculate levels were detected for Ag, Al, Ba, Co, Li, Mo, K, and Mg. Potassium was the most abundant macro-element in the ejaculate, while sodium was the most abundant in blood serum. Among the micro-elements, Al was predominant in both types of samples. It is noteworthy that oxidative status markers including ROS, TAC, and MDA were significantly higher in ejaculate indicating the presence of oxidative stress in <i>C. carpio</i> reproductive tissue. The positive correlations between Mg and Ca in blood serum and ejaculate suggest these elements play a functional role in metabolic and physiological processes. In contrast, the positive correlations of Ba and Al with markers of oxidative stress indicated the association of these metals with induction of oxidative stress. Our findings provide insights into the association of metals with biomarkers of physiological function as well as adverse effects in <i>C. carpio</i>.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"999-1014"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cocoa (<i>Theobroma cacao</i> L.) photobiological effect on human fibroblast cells (HFF-1) exposed to ultraviolet B (UVB) radiation.","authors":"Josiéle da Silva Prade, Camila Medianeira da Silva D'Ávila, Thayline Correia da Silva, Altevir Rossato Viana, André Passaglia Schuch, Isadora Cassel Livinalli, Ana Clara Zanini Bertoncelli, Fernanda Krapf Saccol, Tallys de Oliveira Mendes, Jean Lucas Gutknecht da Silva, Daniela Bitencourt Rosa Leal, Natielen Jacques Schuch, Gabriela Hass de Mello, Mariana Fernandes Ribeiro, Patrícia Gomes, Francine Carla Cadoná","doi":"10.1080/15287394.2024.2439535","DOIUrl":"https://doi.org/10.1080/15287394.2024.2439535","url":null,"abstract":"<p><p>This study aimed to investigate the <i>in vitro</i> photobiological action of cocoa solution on a human fibroblast cell line (HFF-1) exposed to ultraviolet B (UVB) radiation. Three experimental models were utilized, where fibroblast cells were treated with different concentrations of cocoa as follows: 50; 100; 250; 500; 750; 1000 or 1500 µg/ml and concomitantly exposed to UVB 7 kJ/m² for 10 min. The following parameters were examined 1) analysis of the pre-treatment action of cocoa; 2) investigation of the co-treatment activity of cocoa at the time of exposure; and 3) study the effect of cocoa in the post-treatment of the damage initiated by UVB. Cocoa exhibited biological action only in the post-treatment model at almost all tested concentrations compared to cells exposed to UVB alone. Further, fibroblast cells treated only with cocoa displayed higher levels of proliferation as evidenced by a decrease in the levels of basal reactive oxygen species (ROS) and nitric oxide (NO). In addition, cocoa also modulated the cell cycle of cells in the absence of UVB exposure by increasing the G2/M phase, although this did not generate significant changes in cells exposed concomitantly to cocoa and UVB. Therefore, data demonstrated that exposure to cocoa improved fibroblast cell-based control rates and exhibited an important reparative activity against damage initiated by UVB in human fibroblasts. Cocoa may thus be considered as a potential beneficial agent to be utilized in UVB-damaged skin cells.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}