Journal of Toxicology and Environmental Health-Part A-Current Issues最新文献_第5页

Investigation on the mancozeb toxicity in adult zebrafish (Danio rerio). 研究锰锌对成年斑马鱼（Danio rerio）的毒性。

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-08-02 Epub Date: 2024-05-09 DOI: 10.1080/15287394.2024.2352787

Ellen Jaqueline Mendes, Samara Cristina Mazon, Isabella Bodanese Marsaro, Maria Eduarda Hermes, Adrieli Sachett, Kanandra Taisa Bertoncello, Fernando Rafael de Moura, Flavio Manoel Rodrigues da Silva Júnior, Liz Girardi Müller, Cássia Alves Lima-Rezende, Anna Maria Siebel

{"title":"Investigation on the mancozeb toxicity in adult zebrafish (Danio rerio).","authors":"Ellen Jaqueline Mendes, Samara Cristina Mazon, Isabella Bodanese Marsaro, Maria Eduarda Hermes, Adrieli Sachett, Kanandra Taisa Bertoncello, Fernando Rafael de Moura, Flavio Manoel Rodrigues da Silva Júnior, Liz Girardi Müller, Cássia Alves Lima-Rezende, Anna Maria Siebel","doi":"10.1080/15287394.2024.2352787","DOIUrl":"10.1080/15287394.2024.2352787","url":null,"abstract":"Agriculture has gained increasing importance in response to the continuous growth of the world population and constant need for food. To avoid production losses, farmers commonly use pesticides. Mancozeb is a fungicide used in agriculture as this compound is effective in combating fungi that harm crops. However, this fungicide may also produce damage to non-target organisms present in soil and water. Therefore, this study aimed to investigate the influence of exposure to mancozeb on survival rate, locomotor activity, behavior, and oxidative status utilizing adult zebrafish (Danio rerio) as a model following exposure to environmentally relevant concentrations of this pesticide. The experimental groups were negative control, positive control, and mancozeb (0.3; 1.02; 3.47; 11.8 or 40 μg/L). Zebrafish were exposed to the respective treatments for 96 hr. Exposure to mancozeb did not markedly alter survival rate and oxidative status of Danio rerio. At a concentration of 11.8 μg/L, the fungicide initiated changes in locomotor pattern of the animals. The results obtained suggest that the presence of mancozeb in the environment might produce locomotor alterations in adult zebrafish, which subsequently disrupt the animals' innate defense mechanisms. In nature, this effect attributed to mancozeb on non-target organisms might result in adverse population impacts and ecological imbalance.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"616-629"},"PeriodicalIF":2.6,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enriched housing differentially alters allostatic load and cardiopulmonary responses to wildfire-related smoke in male and female mice. 高密度饲养会不同程度地改变雌雄小鼠的代谢负荷和对野火相关烟雾的心肺反应。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-07-17 Epub Date: 2024-05-09 DOI: 10.1080/15287394.2024.2346582

Michelle Fiamingo, Aleah Bailey, Sydnie Toler, Kaleb Lee, Wendy Oshiro, Brendan Yoo, Todd Krantz, Paul Evansky, David Davies, M Ian Gilmour, Aimen Farraj, Ilona Jaspers, Mehdi S Hazari

{"title":"Enriched housing differentially alters allostatic load and cardiopulmonary responses to wildfire-related smoke in male and female mice.","authors":"Michelle Fiamingo, Aleah Bailey, Sydnie Toler, Kaleb Lee, Wendy Oshiro, Brendan Yoo, Todd Krantz, Paul Evansky, David Davies, M Ian Gilmour, Aimen Farraj, Ilona Jaspers, Mehdi S Hazari","doi":"10.1080/15287394.2024.2346582","DOIUrl":"10.1080/15287394.2024.2346582","url":null,"abstract":"Living conditions are an important modifier of individual health outcomes and may lead to higher allostatic load (AL). However, housing-induced cardiovascular and immune effects contributing to altered environmental responsiveness remain understudied. This investigation was conducted to examine the influence of enriched (EH) versus depleted housing (DH) conditions on cardiopulmonary functions, systemic immune responses, and allostatic load in response to a single wildfire smoke (WS) exposure in mice. Male and female C57BL/6J mice were divided into EH or DH for 22 weeks, and cardiopulmonary assessments measured before and after exposures to either one-hr filtered air (FA) or flaming eucalyptus WS exposure. Male and female DH mice exhibited increased heart rate (HR) and left ventricular mass (LVM), as well as reduced stroke volume and end diastolic volume (EDV) one week following exposure to WS. Female DH mice displayed significantly elevated levels of IL-2, IL-17, corticosterone and hemoglobin A1c (HbA1c) following WS, while female in EH mice higher epinephrine levels were detected. Female mice exhibited higher AL than males with DH, which was potentiated post-WS exposure. Thus, DH increased susceptibility to extreme air pollution in a gender-dependent manner suggesting that living conditions need to be evaluated as a modifier of toxicological responses.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"561-578"},"PeriodicalIF":2.3,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11167957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of cytotoxicity, acute toxicity, genotoxicity and antioxidant and antigenotoxicity activities of the sarcotesta fraction of punica granatum L. rich in lectin (PgTel). 评估富含凝集素（PgTel）的石榴果肉部分的细胞毒性、急性毒性、遗传毒性以及抗氧化和抗原毒性活性。

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-07-17 Epub Date: 2024-05-07 DOI: 10.1080/15287394.2024.2351476

Leila Viviany Araújo Coêlho, Elinaldo Francisco de Lima Bento, Wêndeo Kennedy Costa, Magda Rhayanny Assunção Ferreira, Luiz Alberto Lira Soares, Márcia Vanusa da Silva, Patrícia Maria Guedes Paiva, Alisson Macário de Oliveira, Thiago Henrique Napoleão

{"title":"Evaluation of cytotoxicity, acute toxicity, genotoxicity and antioxidant and antigenotoxicity activities of the sarcotesta fraction of punica granatum L. rich in lectin (PgTel).","authors":"Leila Viviany Araújo Coêlho, Elinaldo Francisco de Lima Bento, Wêndeo Kennedy Costa, Magda Rhayanny Assunção Ferreira, Luiz Alberto Lira Soares, Márcia Vanusa da Silva, Patrícia Maria Guedes Paiva, Alisson Macário de Oliveira, Thiago Henrique Napoleão","doi":"10.1080/15287394.2024.2351476","DOIUrl":"10.1080/15287394.2024.2351476","url":null,"abstract":"Punica granatum, popularly known as pomegranate, is a fruit tree with wide worldwide distribution, containing numerous phytochemicals of great medicinal value. The aim of the present study was to determine the phytochemical profile and antioxidant potential of a protein fraction (PF) derived from P. granatum sarcotesta which is rich in lectin. In addition, the acute oral toxicity, genotoxicity and antigenotoxicity of this protein fraction (PF) from P. granatum sarcotesta was measured. The phytochemical profile of PF was determined using HPLC. The in vitro antioxidant effect was assessed using the methods of total antioxidant capacity (TAC) and DPPH and ABTS+ radical scavenging. Acute oral toxicity was determined in female Swiss mice administered a single dose of 2000 mg/kg. This PF was examined for genotoxicity and antigenotoxicity at doses of 500, 1000 and 2000 mg/kg, utilizing mouse peripheral blood cells. Phytochemical characterization detected a high content of ellagic acid and antioxidant capacity similar to that of ascorbic acid (positive control). PF was not toxic (LD50 >2000 mg/kg) and did not exert a genotoxic effect in mice. PF protected the DNA of peripheral blood cells against damage induced by cyclophosphamide. In conclusion, this PF fraction exhibited significant antioxidant activity without initiating toxic or genotoxic responses in mice.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"592-603"},"PeriodicalIF":2.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivity of hydroalcoholic extracts from tropaeolum majus L. (tropaeolaceae) on the germination, initial plant development and cell cycle of Lactuca sativa L. 水醇提取物对莴苣萌芽、植物初始发育和细胞周期的生物活性

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-07-17 Epub Date: 2024-05-06 DOI: 10.1080/15287394.2024.2349107

Fabio Eduardo Dos Santos, Marcos Schleiden Sousa Carvalho, Maria Das GraçGraçAs Cardoso, Luciane Resende Vilela, Larissa Fonseca Andrade-Vieira

{"title":"Bioactivity of hydroalcoholic extracts from tropaeolum majus L. (tropaeolaceae) on the germination, initial plant development and cell cycle of Lactuca sativa L.","authors":"Fabio Eduardo Dos Santos, Marcos Schleiden Sousa Carvalho, Maria Das GraçGraçAs Cardoso, Luciane Resende Vilela, Larissa Fonseca Andrade-Vieira","doi":"10.1080/15287394.2024.2349107","DOIUrl":"10.1080/15287394.2024.2349107","url":null,"abstract":"Natural products are usually considered harmless; however, these substances need to be consumed with caution. Biological assays with plant models are a suitable alternative for prospective studies to assess natural product-initiated toxicity. The aim of this study was to examine the toxic potential of leaf and flower extracts derived from Tropaeolum majus L. a widely used plant in traditional medicine. Seeds of Lactuca sativa L. were exposed to T. majus extracts and based upon the seedling growth curve values, the 50% Inhibition Concentration (IC50) was calculated and applied for cell cycle analysis exposure. Both extracts contained organic acids, proteins, amino acids, and terpene steroids. Sesquiterpene lactones and depside were detected in leaf extracts. The higher concentration tested exhibited a marked phytotoxic effect. The extracts induced clastogenic, aneugenic cytotoxic, and potential mutagenic effects. The possible relationships between the classes of compounds found in the extracts and effects on cells and DNA were determined.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"579-591"},"PeriodicalIF":2.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating salt stress by conditioning seeds with ultraviolet light (UV-C) in white oats (Avena sativa L.). 用紫外线（UV-C）调节白燕麦（Avena sativa L.）种子，减轻盐胁迫。

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-07-02 Epub Date: 2024-04-25 DOI: 10.1080/15287394.2024.2345878

Raquel Stefanello, Raissa Tainá Puntel, Wagner Jesus da Silva Garcia, Lucio Strazzabosco Dorneles

{"title":"Mitigating salt stress by conditioning seeds with ultraviolet light (UV-C) in white oats (Avena sativa L.).","authors":"Raquel Stefanello, Raissa Tainá Puntel, Wagner Jesus da Silva Garcia, Lucio Strazzabosco Dorneles","doi":"10.1080/15287394.2024.2345878","DOIUrl":"https://doi.org/10.1080/15287394.2024.2345878","url":null,"abstract":"Seed conditioning with ultraviolet light (UV-C) might (1) improve crop yield and quality, (2) reduce the use of agrochemicals during cultivation, and (3) increase plant survival in high salinity environments. The aim of this study was to examine the effects of UV-C conditioning of white oat seeds at two doses (0.85 and 3.42 kJ m-2) under salinity stress (100 mM NaCl). Seeds were sown on germination paper and kept in a germination chamber at 20°C. Germination and seedling growth parameters were evaluated after 5 and 10 days. Data demonstrated that excess salt reduced germination and initial growth of white oat seedlings. In all the variables analyzed, exposure of seeds to UV-C under salt stress exerted a positive effect compared to non-irradiated control. The attenuating influence of UV-C in germination was greater at 0.85 than at 3.42 kJ m-2. Thus, data indicate that conditioning white oat seeds in UV-C light produced greater tolerance to salt stress. These findings suggest that UV-C conditioning of white oat seeds may be considered as a simple and economical strategy to alleviate salt-induced stress.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":"87 13","pages":"533-540"},"PeriodicalIF":2.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of skin sensitization potential of zinc oxide, aluminum oxide, manganese oxide, and copper oxide nanoparticles through the local lymph node assay: 5-bromo-deoxyuridine flow cytometry method. 通过局部淋巴结试验评估氧化锌、氧化铝、氧化锰和氧化铜纳米粒子的皮肤致敏潜力：5-溴脱氧尿苷流式细胞仪法。

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-05-26 DOI: 10.1080/15287394.2024.2357466

Anju Maharjan, Ravi Gautam, GiYong Lee, DongYoon Kim, DaEun Lee, Manju Acharya, HyoungAh Kim, Yong Heo, ChangYul Kim

{"title":"Assessment of skin sensitization potential of zinc oxide, aluminum oxide, manganese oxide, and copper oxide nanoparticles through the local lymph node assay: 5-bromo-deoxyuridine flow cytometry method.","authors":"Anju Maharjan, Ravi Gautam, GiYong Lee, DongYoon Kim, DaEun Lee, Manju Acharya, HyoungAh Kim, Yong Heo, ChangYul Kim","doi":"10.1080/15287394.2024.2357466","DOIUrl":"https://doi.org/10.1080/15287394.2024.2357466","url":null,"abstract":"The advent of nanotechnology has significantly spurred the utilization of nanoparticles (NPs) across diverse sectors encompassing industry, agriculture, engineering, cosmetics, and medicine. Metallic oxides including zinc oxide (ZnO), copper oxide (CuO), manganese oxide (Mn2O3), and aluminum oxide (Al2O3), in their NP forms, have become prevalent in cosmetics and various dermal products. Despite the expanding consideration of these compounds for dermal applications, their potential for initiating skin sensitization (SS) has not been comprehensively examined. An in vivo assay, local lymph node assay: 5-bromo-2-deoxyuridine-flow cytometry method (LLNA: BrdU-FCM) recognized as an alternative testing method for screening SS potential was used to address these issues. Following the OECD TG 442B guidelines, NPs suspensions smaller than 50 nm size were prepared for ZnO and Al2O3 at concentrations of 10, 25, and 50%, and Mn2O3 and CuO at concentrations of 5, 10, and 25%, and applied to the dorsum of each ear of female BALB/c mice on a daily basis for 3 consecutive days. Regarding the prediction of test substance to skin sensitizer if sensitization index (SI)≥2.7, all 4 NPs were classified as non-sensitizing. The SI values were below 2.06, 1.33, 1.42, and 0.99 for ZnO, Al2O3, Mn2O3, and CuO, respectively, at all test concentrations. Although data presented were negative with respect to adverse SS potential for these 4 NPs, further confirmatory tests addressing other key events associated with SS adverse outcome pathway need to be carried out to arrive at an acceptable conclusion on the skin safety for both cosmetic and dermal applications.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-11"},"PeriodicalIF":2.6,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms underlying LncRNA SNHG1 regulation of Alzheimer's disease involve DNA methylation. LncRNA SNHG1调控阿尔茨海默病的机制涉及DNA甲基化。

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-05-18 Epub Date: 2024-03-29 DOI: 10.1080/15287394.2024.2334248

Hong Chen, Chun-Jie Zhang, Zhi-Ying Zhao, Yang-Yang Gao, Jian-Tian Zhao, Xiao-Xu Li, Ming Zhang, He Wang

{"title":"Mechanisms underlying LncRNA SNHG1 regulation of Alzheimer's disease involve DNA methylation.","authors":"Hong Chen, Chun-Jie Zhang, Zhi-Ying Zhao, Yang-Yang Gao, Jian-Tian Zhao, Xiao-Xu Li, Ming Zhang, He Wang","doi":"10.1080/15287394.2024.2334248","DOIUrl":"10.1080/15287394.2024.2334248","url":null,"abstract":"Alzheimer's disease (AD) is a neurodegenerative disease associated with long non-coding RNAs and DNA methylation; however, the mechanisms underlying the role of lncRNA small nucleolar RNA host gene 1 (lncRNA SNHG1) and subsequent involvement of DNA methylation in AD development are not known. The aim of this study was to examine the regulatory mechanisms attributed to lncRNA SNHG1 gene utilizing 2 strains of senescence-accelerated mouse prone 8 (SAMP8) model of AD and compared to senescence-accelerated mouse resistant (SAMR) considered a control. Both strains of the mouse were transfected with either blank virus, psLenti-U6-SNHG1(low gene expression) virus, and psLenti-pA-SNHG1(gene overexpression) virus via a single injection into the brains for 2 weeks. At 2 weeks mice were subjected to a Morris water maze to determine any behavioral effects followed by sacrifice to extract hippocampal tissue for Western blotting to measure protein expression of p-tau, DNMT1, DNMT3A, DNMT3B, TET1, and p-Akt. No marked alterations were noted in any parameters following blank virus transfection. In SAMP8 mice, a significant decrease was noted in protein expression of DNMT1, DNMT3A, DNMT3B, and p-Akt associated with rise in p-tau and TET1. Transfection with ps-Lenti-U6-SNHG1 alone in SAMR1 mice resulted in a significant rise in DNMTs and p-Akt and a fall in p-tau and TET1. Transfection of SAMP8 with ps-Lenti-U6-SNHG1 blocked effects on overexpression noted in this mouse strain. However, knockdown of lncRNA SNHG1 yielded the opposite results as found in SAMR1 mice. In conclusion, the knockdown of lncRNA SNHG1 enhanced DNA methylation through the PI3K/Akt signaling pathway, thereby reducing the phosphorylation levels of tau in SAMP8 AD model mice with ameliorating brain damage attributed to p-tau accumulation with consequent neuroprotection.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"428-435"},"PeriodicalIF":2.6,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-05-18 Epub Date: 2023-11-20 DOI: 10.1080/15287394.2023.2284009

引用次数: 0

Effects of schisandrin B on hypoxia-related cognitive function and protein expression in vascular dementia rats. 五味子素 B 对血管性痴呆大鼠缺氧相关认知功能和蛋白质表达的影响

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-05-18 Epub Date: 2024-03-29 DOI: 10.1080/15287394.2024.2334247

Jing-Bo He, He Zhang, Hong-Xia Zheng, Jian-Xin Jia, Yi-Chi Zhang, Xu-Sheng Yan, Xiao-Xu Li, Kai-Wen Wei, Jun Mao, Hong Chen, Jing Li, He Wang, Ming Zhang, Zhi-Ying Zhao

{"title":"Effects of schisandrin B on hypoxia-related cognitive function and protein expression in vascular dementia rats.","authors":"Jing-Bo He, He Zhang, Hong-Xia Zheng, Jian-Xin Jia, Yi-Chi Zhang, Xu-Sheng Yan, Xiao-Xu Li, Kai-Wen Wei, Jun Mao, Hong Chen, Jing Li, He Wang, Ming Zhang, Zhi-Ying Zhao","doi":"10.1080/15287394.2024.2334247","DOIUrl":"10.1080/15287394.2024.2334247","url":null,"abstract":"Vascular dementia (VD) a heterogenous group of brain disorders in which cognitive impairment is attributable to vascular risk factors and cerebrovascular disease. A common phenomenon in VD is a dysfunctional cerebral regulatory mechanism associated with insufficient cerebral blood flow, ischemia and hypoxia. Under hypoxic conditions oxygen supply to the brain results in neuronal death leading to neurodegenerative diseases including Alzheimer's (AD) and VD. In conditions of hypoxia and low oxygen perfusion, expression of hypoxia-inducible factor 1 alpha (HIF-1α) increases under conditions of low oxygen and low perfusion associated with upregulation of expression of hypoxia-upregulated mitochondrial movement regulator (HUMMR), which promotes anterograde mitochondrial transport by binding with trafficking protein kinesin 2 (TRAK2). Schisandrin B (Sch B) an active component derived from Chinese herb Wuweizi prevented β-amyloid protein induced morphological alterations and cell death using a SH-SY5Y neuronal cells considered an AD model. It was thus of interest to determine whether Sch B might also alleviate VD using a rat bilateral common carotid artery occlusion (BCAO) dementia model. The aim of this study was to examine the effects of Sch B in BCAO on cognitive functions such as Morris water maze test and underlying mechanisms involving expression of HIF-1α, TRAK2, and HUMMR levels. The results showed that Sch B improved learning and memory function of rats with VD and exerted a protective effect on the hippocampus by inhibition of protein expression of HIF-1α, TRAK2, and HUMMR factors. Evidence indicates that Sch B may be considered as an alternative in VD treatment.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"421-427"},"PeriodicalIF":2.6,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective effect and mechanism of glycosides of cistanche deserticola on rats in middle cerebral artery occlusion (MCAO) model. 肉苁蓉苷对大脑中动脉闭塞（MCAO）模型大鼠的保护作用及其机制

IF 2.6 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2024-05-18 Epub Date: 2024-04-01 DOI: 10.1080/15287394.2024.2337365

Lu Wang, Jian-Xin Jia, Shi-Bin Zhang, Wei Song, Xu-Sheng Yan, Dong-Sheng Huo, He Wang, Li-E Wu, Zhan-Jun Yang

{"title":"The protective effect and mechanism of glycosides of cistanche deserticola on rats in middle cerebral artery occlusion (MCAO) model.","authors":"Lu Wang, Jian-Xin Jia, Shi-Bin Zhang, Wei Song, Xu-Sheng Yan, Dong-Sheng Huo, He Wang, Li-E Wu, Zhan-Jun Yang","doi":"10.1080/15287394.2024.2337365","DOIUrl":"10.1080/15287394.2024.2337365","url":null,"abstract":"Cerebral ischemia-reperfusion injury (CIRI) occurs frequently clinically as a complication following cardiovascular resuscitation resulting in neuronal damage specifically to the hippocampal CA1 region with consequent cognitive impairment. Apoptosis and oxidative stress were proposed as major risk factors associated with CIRI development. Previously, glycosides obtained from Cistanche deserticola (CGs) were shown to play a key role in counteracting CIRI; however, the underlying mechanisms remain to be determined. This study aimed to investigate the neuroprotective effect of CGs on subsequent CIRI in rats. The model of CIRI was established for 2 hr and reperfusion for 24 hr by middle cerebral artery occlusion (MCAO) model. The MCAO rats were used to measure the antioxidant and anti-apoptotic effects of CGs on CIRI. Neurological function was evaluated by the Longa neurological function score test. 2,3,5-Triphenyltetrazolium chloride (TTC) staining was used to detect the area of cerebral infarction. Nissl staining was employed to observe neuronal morphology. TUNEL staining was used to detect neuronal apoptosis, while Western blot determined protein expression levels of factors for apoptosis-related and PI3K/AKT/Nrf2 signaling pathway. Data demonstrated that CGs treatment improved behavioral performance, brain injury, and enhanced antioxidant and anti-apoptosis in CIRI rats. In addition, CGs induced activation of PI3K/AKT/Nrf2 signaling pathway accompanied by inhibition of the expression of apoptosis-related factors. Evidence indicates that CGs amelioration of CIRI involves activation of the PI3K/AKT/Nrf2 signaling pathway associated with increased cellular viability suggesting these glycosides may be considered as an alternative compound for CIRI treatment.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"448-456"},"PeriodicalIF":2.6,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140337715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0