Journal of Toxicology and Environmental Health-Part A-Current Issues最新文献_第4页

Genotoxicity of pure compounds and commercial formulations of the maize herbicides mesotrione and s-metolachlor in Vicia faba L. 玉米除草剂甲三酮和s-异甲草胺纯化合物和商业配方对蚕豆的遗传毒性。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-05-12 DOI: 10.1080/15287394.2025.2502965

Larissa Fonseca Andrade-Vieira, Sylvie Cotelle

{"title":"Genotoxicity of pure compounds and commercial formulations of the maize herbicides mesotrione and s-metolachlor in Vicia faba L.","authors":"Larissa Fonseca Andrade-Vieira, Sylvie Cotelle","doi":"10.1080/15287394.2025.2502965","DOIUrl":"https://doi.org/10.1080/15287394.2025.2502965","url":null,"abstract":"Herbicides containing the active components mesotrione and S-metolachlor persist in the environment and adversely affect non-target organisms. Therefore, it was of interest to examine the toxicological risk associated with these compounds. This study aimed to investigate the toxic effects of the active pure compounds mesotrione and S-metolachlor and their respective commercial formulations as evidenced by MN bioassays utilizing Vicia faba L. (broad bean) as a model. The experiments followed the 48-hr protocol of ISO 29200. Dilutions of the active components and their commercial formulations, ranging from 1.56 to 50 mg/L, were tested. Hoagland's solution was used as a negative control, and maleic hydrazide as a positive control. A decrease in mitotic index (MI) was observed with the pure compounds, where mesotrione significantly reduced the frequency of cell division at concentrations >12.5 mg/L, and S-metolachlor initiated a significant fall at only the highest concentration (50 mg/L). In addition, the MN frequency was markedly increased at treatments of 3.125 mg/L mesotrione and 6.25 to 25 mg/L S-metolachlor. However, herbicide treatments with the commercial formulation containing the same concentrations of active compounds did not significantly damage plant DNA. Taken together, the findings demonstrated that in commercial formulations, the combined effects of the pure compounds and solvents or other substances included in the product might occur and consequently interfere with the genotoxic potential of the active compounds. Although the cytotoxicity was of the same order of magnitude for both S-metolachlor and mesotrione, mesotrione alone appears to be more genotoxic than S-metolachlor.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-13"},"PeriodicalIF":2.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuating effects of vitamin C on lead (Pb)-induced toxicological and endocrine responses in male Wistar rats. 维生素C对铅诱导的雄性Wistar大鼠毒理学和内分泌反应的减弱作用。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-05-06 DOI: 10.1080/15287394.2025.2501016

Ukam U Uno, Oju R Ibor, Utip B Ekaluo, Monday M Onakpa, Augustine Arukwe

{"title":"Attenuating effects of vitamin C on lead (Pb)-induced toxicological and endocrine responses in male Wistar rats.","authors":"Ukam U Uno, Oju R Ibor, Utip B Ekaluo, Monday M Onakpa, Augustine Arukwe","doi":"10.1080/15287394.2025.2501016","DOIUrl":"https://doi.org/10.1080/15287394.2025.2501016","url":null,"abstract":"The aim of this study was to investigate the attenuating effect of vitamin C (VC) on lead (Pb)-induced toxicity in male Wistar rats. Twenty-four mature male rats were divided into 4 groups consisting of 6 animals using a completely randomized design. Rats were treated orally with either 60 mg/kg body weight (BW) Pb acetate or 100 mg/kg BW VC singly or combination. Controls group received water (vehicle control). After 65 days exposure, testes and epididymis of the animals were measured for testicular weight, epididymal weight and sperm estimations, while blood and liver samples were collected for liver function, hormonal assays, and oxidative stress responses including transcript expression using real-time PCR. Lead initiated significant increases in expression and activities of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD,) glutathione peroxidase (Gpx) and levels of malondialdehyde (MDA) and nitric oxide (NO). Liver function enzyme activities for alkaline phosphatase (ALP,) aspartate aminotransferase (AST), and alanine aminotransferase (ALP) were also elevated in Pb exposed rats. In contrast, a significant decrease was noted in reproductive hormones testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), sperm variables including motility, viability and counts, as well as testes and epididymis weights following Pb treatment. However, the oxidative stress responses and liver function enzymes were reduced in the combined Pb and VC exposure group, while reproductive hormones and sperm profile increased significantly compared to Pb alone. Data indicate that VC exerts an attenuating effect. Our findings showed the attenuating potential of VC on Pb-induced toxicity of male rats.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between long-term exposure to fine particulate air pollution and colorectal cancer mortality in Taiwan. 台湾地区长期暴露于空气细颗粒物污染与结直肠癌死亡率之关系

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-24 DOI: 10.1080/15287394.2025.2497074

ChunYuh Yang

{"title":"Relationship between long-term exposure to fine particulate air pollution and colorectal cancer mortality in Taiwan.","authors":"ChunYuh Yang","doi":"10.1080/15287394.2025.2497074","DOIUrl":"https://doi.org/10.1080/15287394.2025.2497074","url":null,"abstract":"The International Agency for Research on Cancer classified fine particulate matter (PM2.5) air pollution as carcinogenic to humans (Group I). Although PM2.5 exposure has been associated with lung cancer occurrence, few studies investigated this association with non-lung cancer. Colorectal cancer (CRC) is the third leading cause of cancer deaths both among men and women. In Taiwan, deaths attributed to CRC vary considerably across townships, suggesting involvement of the environment. The aim of this study was to examine the association between long-term ambient PM2.5 exposure and deaths attributed to CRC in 66 municipal areas across Taiwan. Annual PM2.5 levels were compared against age-standardized CRC mortality rates in male and female residents of these municipalities from 2012 to 2021. Annual PM2.5 levels of different municipalities were sub-divided into tertiles. Adjusted risk ratio (RR) was calculated by multiple regression analyses, controlling for municipal lung cancer deaths, urbanization level, annual average household income, and density of physicians in the municipal areas. For males, adjusted RRs for CRC death were 1.1 (95% CI = 1.05-1.15) for municipalities with PM2.5 levels ranging from 18.96 to 25.19 µg/m3and 1.15 (95% CI = 1.1-1.21) for levels ranging from 25.2 to 29.48 µg/m3, respectively, compared to those areas belonging to the lowest tertiles. Our analysis of trend suggested that risk of CRC-related death paralleled increases PM2.5 levels in males. For females, adjusted RRs were 1.18 (95% CI = 1.12-1.25) and 1.12 (95% CI = 1.06-1.19), respectively. Evidence indicated that long-term exposure to PM2.5 may elevate the risk of CRC-related death in both men and women in Taiwan.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-10"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144058362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MC-LR induced apoptosis in human embryonic kidney (HEK293) cells through activation of TNF-R1/RIPK1 pathway. MC-LR通过激活TNF-R1/RIPK1通路诱导人胚胎肾（HEK293）细胞凋亡。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-18 Epub Date: 2024-12-20 DOI: 10.1080/15287394.2024.2439532

Yaqi Wang, Yue Yang, Mei Yuan, Fei Yang, Wen Zeng, Jun Liu, Chunhua Zhan

{"title":"MC-LR induced apoptosis in human embryonic kidney (HEK293) cells through activation of TNF-R1/RIPK1 pathway.","authors":"Yaqi Wang, Yue Yang, Mei Yuan, Fei Yang, Wen Zeng, Jun Liu, Chunhua Zhan","doi":"10.1080/15287394.2024.2439532","DOIUrl":"10.1080/15287394.2024.2439532","url":null,"abstract":"In recent years, the outbreak of cyanobacterial blooms has become increasingly frequent. Microcystin-LR (MC-LR), a metabolite of cyanobacteria, poses a significant threat to the ecosystem and human health. Several studies have demonstrated that MC-LR might induce renal cell apoptosis, as a consequence of tissue damage. However, the molecular mechanisms underlying MC-LR-initiated renal injury remain to be determined. This investigation aimed to determine the role of apoptosis in MC-LR-induced kidney damage and its potential underlying mechanisms using the human embryonic kidney (HEK293) cell line. The results of TUNEL and immunofluorescence assays indicated that MC-LR induced increased apoptosis in HEK293 cells. Compared to control, the mRNA expression levels of RIPK1, caspase-8, and TNF-α were elevated following incubation with MC-LR, while the mRNA expression level of Bcl-2/Bax was decreased. The protein levels of RIPK1, TNF-R1, and caspase-8 were elevated in the MC-LR-treated HEK293 cells. Data demonstrated that MC-LR induced renal cell apoptosis through activation of the TNF-R1/RIPK1 pathway, providing new insights into understanding the toxic mechanisms attributed to MC-LR.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"339-348"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of 2,5-hexanedione on rat ovarian granulosa cell apoptosis involves endoplasmic reticulum stress-dependent m-TOR signaling pathway. 2,5-己二酮对大鼠卵巢颗粒细胞凋亡的影响涉及内质网应激依赖的m-TOR信号通路。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-18 Epub Date: 2024-12-12 DOI: 10.1080/15287394.2024.2438832

Lemei Zhu, Yue Yang, Jingsi Tan, Yibo Lin, Jiaqi Qing, Xin Li, Lingfeng Zeng

{"title":"Effect of 2,5-hexanedione on rat ovarian granulosa cell apoptosis involves endoplasmic reticulum stress-dependent m-TOR signaling pathway.","authors":"Lemei Zhu, Yue Yang, Jingsi Tan, Yibo Lin, Jiaqi Qing, Xin Li, Lingfeng Zeng","doi":"10.1080/15287394.2024.2438832","DOIUrl":"10.1080/15287394.2024.2438832","url":null,"abstract":"Occupational exposure to N-hexane/2,5-hexanedione (2,5-HD) was found to adversely affect reproductive functions in females. However, there are few studies regarding the mechanisms underlying reproductive system damage initiated by 2,5-HD. Several studies demonstrated that 2,5-HD exerts hormonal dysfunctions in females by promoting apoptosis using rat ovarian granulosa cells (GCs) as a model. The endoplasmic reticulum (ER) plays a key role in cellular processes such as protein folding and modification, Ca2+ storage, and lipid synthesis, which are known to involve the activation of stress (ERS)-dependent m-TOR signaling pathway. Thus, the aim of this study was to examine the effects of 2,5-HD on ER and the associated activation of stress (ERS)-dependent m-TOR signaling pathway resulting in consequent apoptosis of ovarian GCs. Data demonstrated that after intraperitoneal treatment with 100, 200, or 400 mg/kg 2,5-HD for 6 consecutive weeks, 5 times per week, a decrease in body weight, ovarian weight, and relative ovary weight was found. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay showed that 2,5-HD promoted apoptosis of ovarian GCs, which involved enhanced relative protein expression levels of m-TOR/p-mTOR. Our findings demonstrated that 2,5-HD (1) elevated expression levels of pro-apoptosis-related genes Bax and Caspase 3, (2) decreased expression levels of the anti-apoptosis gene Bcl-2, and (3) activated the protein expression of glucose-regulatory protein 78 (GRP78), inositol-requiring enzyme-1 (IRE1), and c-Jun terminal kinase (JNK) associated with increased apoptosis. Evidence indicates that chronic exposure to 2,5-HD induced apoptosis of ovarian GCs, and the possible mechanism underlying this effect involves the ERS-dependent m-TOR signaling pathway.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"319-328"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of a native bacterium Citrobacter farmeri against microcystin-LR in anaerobic environments. 厌氧环境中农柠檬酸杆菌抗微囊藻毒素- lr的分离鉴定。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-18 Epub Date: 2024-12-30 DOI: 10.1080/15287394.2024.2438837

Rong Zhang, Lili Yang, Sihong Long, Shengyu Zhang, Jia Wei, Fei Yang

{"title":"Isolation and identification of a native bacterium Citrobacter farmeri against microcystin-LR in anaerobic environments.","authors":"Rong Zhang, Lili Yang, Sihong Long, Shengyu Zhang, Jia Wei, Fei Yang","doi":"10.1080/15287394.2024.2438837","DOIUrl":"10.1080/15287394.2024.2438837","url":null,"abstract":"Microcystin-LR (MC-LR), produced by cyanobacterial harmful algal blooms, poses a serious threat to aquatic ecosystems and human health. Biodegradation is an important method for MC-LR elimination. Aerobic biodegradation has been extensively studied and many bacteria were identified. However, few MC-degrading bacteria have been isolated from anaerobic environments, and these degradation mechanisms are poorly understood. The aim of this study was to collect anaerobic MC-degrading bacteria from lake sediments in Lake Taihu using acclimation culture to assess biodegradation. Five strains with MC-LR degradation ability were isolated, with strain A4 belonging to Citrobacter farmeri (C. farmeri). exhibiting the highest efficiency at 0.486 μg/ml/d. High-performance liquid chromatography (HPLC) identified two novel MC-LR degradation products. Further polymerase chain reaction (PCR) analysis suggested that C. farmeri A4 did not possess the known MC-degrading gene mlrABCD, suggesting the involvement of an mlrABCD-independent anaerobic degradation pathway. Data demonstrated that the bacterial strain C. farmeri A4 found in Lake Taihu exhibited high anaerobic MC-LR degradation properties, which indicated that anaerobic biodegradation may constitute an important biological method for MC-LR removal in natural environment.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":"88 8","pages":"329-338"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of ambient particulate matter (PM_2.5) with reduced sex ratio at birth after the implementation of a rapid transit system in Taipei, Taiwan. 台北市快速交通系统实施后，环境颗粒物（PM2.5）与出生性别比降低的关系。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-03 Epub Date: 2024-12-04 DOI: 10.1080/15287394.2024.2436992

Shang-Shyue Tsai, Yi-Hao Weng, Chun-Yuh Yang

{"title":"Association of ambient particulate matter (PM2.5) with reduced sex ratio at birth after the implementation of a rapid transit system in Taipei, Taiwan.","authors":"Shang-Shyue Tsai, Yi-Hao Weng, Chun-Yuh Yang","doi":"10.1080/15287394.2024.2436992","DOIUrl":"10.1080/15287394.2024.2436992","url":null,"abstract":"Sex ratio at birth (SRB) is a simple, noninvasive way to monitor the reproductive health of a population. Apparently only a few studies have assessed the relationship between ambient air pollution and SRBs. The studies that investigated this relationship have yielded inconsistent results. Ambient air pollution levels, including particulate matter (PM2.5) levels, have decreased in Taipei city after a mass rapid transit (MRT) system commenced operations in 1996. The aim of this study was to determine whether the changes in levels of PM2.5 after the implementation of the MRT system in Taipei may have influenced the concurrent alterations noted in the SRB. In order to assess this potential association, annual numbers of male and female births were obtained from Taiwan's Department of Household Registration, Ministry of Interior Affairs from 1992 to 2023. Mean yearly SRBs were calculated for 4 time periods, 1992-1995, 1996-2004, 2005-2015, and 2016-2023. Linear logistic regression was employed to evaluate the variation in officially reported PM2.5 levels during those periods and alterations in the ratios. The ratios for time periods 1 to 4 were 52.61%, 52.22%, 51.78%, and 51.67%, respectively. Compared to Period 1 (pre-MRT), the odds ratios for Periods 2, 3, and 4 were 0.98 (0.97-0.99), 0.97 (0.96-0.98), and 0.96 (0.95-0.98), respectively. The trend toward the decreased SRB was found to be gradual but significant.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"310-317"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conditioning rice seeds with chitosan to mitigate salt stress. 壳聚糖调节水稻种子以减轻盐胁迫。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-03 Epub Date: 2024-12-24 DOI: 10.1080/15287394.2024.2434656

Raquel Stefanello, Raissa Tainá Puntel, Daiane Balconi Bevilaqua, Wagner Jesus da Silva Garcia, Cristiano Rodrigo Bohn Rhoden, Lucio Strazzabosco Dorneles

{"title":"Conditioning rice seeds with chitosan to mitigate salt stress.","authors":"Raquel Stefanello, Raissa Tainá Puntel, Daiane Balconi Bevilaqua, Wagner Jesus da Silva Garcia, Cristiano Rodrigo Bohn Rhoden, Lucio Strazzabosco Dorneles","doi":"10.1080/15287394.2024.2434656","DOIUrl":"10.1080/15287394.2024.2434656","url":null,"abstract":"Rice is considered to be moderately salt-tolerant during germination, development, and ripening stages, and environmentally sensitive during seedling and reproductive stages, which affects seedling emergence and growth, resulting in significant yield losses. Seed conditioning with chitosan has been employed as a useful tool in high-salinity environments with the aim of increasing crop productivity and quality, as well as promoting more sustainable agricultural practices. Therefore, this study aimed to examine the effect of seed conditioning with chitosan on seed germination and rice seedling growth under salinity stress. The experiment consisted of three seeds conditioning and 4 salinity levels, arranged in a completely randomized design with 4 replications. Seeds were sown on germitest paper, and the rolls were placed in a germination chamber (25 ± 2°C and 12 hr photoperiod). Germination and seedling growth parameters were determined. The high salt concentration resulted in reduced growth of rice seedlings, and exogenous application of chitosan at different concentrations and soaking times exerted no apparent adverse effect on germination and growth variables. The attenuating effect of chitosan was observed in the length of the seedlings at all the concentrations utilized. Therefore, evidence indicates that conditioning rice seeds with chitosan might serve as an alternative to mitigate the adverse effects of exposure to stress induced by high salt concentrations.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"279-290"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure to MC-LR activates the RAF/ERK signaling pathway, leading to renal inflammation and tissue damage in mice. 暴露于MC-LR会激活RAF/ERK信号通路，导致小鼠肾脏炎症和组织损伤。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-03 Epub Date: 2024-12-23 DOI: 10.1080/15287394.2024.2435632

Yiwen Hou, Ying Liu, Yue Yang, Shuaishuai Xu, Fei Yang

{"title":"Exposure to MC-LR activates the RAF/ERK signaling pathway, leading to renal inflammation and tissue damage in mice.","authors":"Yiwen Hou, Ying Liu, Yue Yang, Shuaishuai Xu, Fei Yang","doi":"10.1080/15287394.2024.2435632","DOIUrl":"10.1080/15287394.2024.2435632","url":null,"abstract":"Exposure to microcysatin-LR (MC-LR) is known to result in kidney damage, however the underlying mechanisms involved in MC-LR-initiated renal injury are not known. Thus, the aim of this study was to examine the effects of exposure to MC-LR on human embryo kidney (HEK 293) cell in vitro and male C57BL/6 in vivo. In the in vitro study, HEK 293 cells were incubated with MC-LR (20 µM) for 24 hr. Treatment with MC-LR significantly increased the protein expression of RAF and ERK as well as mRNA expression levels of inflammatory cytokines TNF-α, IL-6, and IL-1β. These findings were confirmed when HEK 293 cells were co-incubated with ERK inhibitor U0126 and MC-LR demonstrating a decrease in protein expression of RAF, ERK, and mRNA levels of pro-inflammatory cytokines. Male C57BL/6 mice were intraperitoneally (ip) injected with MC-LR (20 µg/kg) daily for 21 days. Histopathological analysis demonstrated significant glomerular and tubular damage with inflammatory infiltration. The expression levels of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β were significantly elevated following MC-LR treatment. Administration of MC-LR asignificantly enhanced the protein phosphorylation levels of RAF and ERK. Data demonstrated that exposure to MC-LR induced morphological tissue damage and renal inflammatory reactions by activating the RAF/ERK signaling pathway.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"301-309"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of chronic low-dose microcystin-LR exposure on jejunum apoptosis via RAF/ERK signaling pathway in mouse. 慢性低剂量微囊藻毒素lr通过RAF/ERK信号通路对小鼠空肠细胞凋亡的影响。

IF 2.3 4区医学

Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2025-04-03 Epub Date: 2024-12-12 DOI: 10.1080/15287394.2024.2435631

Sihong Long, Cong Wen, Wen Zeng, Yue Yang, Fei Yang

{"title":"Effect of chronic low-dose microcystin-LR exposure on jejunum apoptosis via RAF/ERK signaling pathway in mouse.","authors":"Sihong Long, Cong Wen, Wen Zeng, Yue Yang, Fei Yang","doi":"10.1080/15287394.2024.2435631","DOIUrl":"10.1080/15287394.2024.2435631","url":null,"abstract":"Microcystin-LR (MC-LR), a class of cyclic heptapeptide compounds synthesized by cyanobacterial species, presents a significant risk to ecological systems and public health. Exposure to MC-LR was found to induce damage to various organs. One of the target organ systems affected by MC-LR is the gastrointestinal tract (GIT). However, the majority of studies regarding GIT focused on colorectal toxicity, with little attention paid to small intestinal toxic injuries, in particular jejunum. Thus, the aim of this study was to investigate the effects attributed to MC-LR exposure on apoptosis and underlying mechanisms utilizing a mouse jejunum injury model following chronic low-dose MC-LR treatment. A total of 40 C57BL/6 male mice were randomly divided into 4 groups with each group receiving drinking water containing 0, 1, 60, or 120 µg/L MC-LR for a duration of 12 months. Results indicated that exposure to MC-LR induced pathological alterations in jejunal tissue as evidenced by abnormal villous serration, crypt disorganization, and lymphocyte infiltration. TUNEL assays demonstrated a significant increase in apoptotic cell count in the 60 and 120 µg/L groups. The 60 and 120 µg/L MC-LR treatment groups exhibited elevated mRNA expression of Bax accompanied by significant reduction in mRNA expression of Bcl-2. The protein levels of cleaved caspase-3 were markedly elevated in the 60 and 120 µg/L MC-LR groups. The protein expression levels of p-RAF and p-ERK were significantly increased in the 60 and 120 µg/L MC-LR treatment groups. Data demonstrated suggest that the RAF/ERK signaling pathway may be involved in MC-LR- induced jejunal apoptosis.","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"291-300"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0