Journal of Toxicology and Environmental Health-Part A-Current Issues最新文献

{"title":"Physiological response of rice to graphene oxide under cadmium chloride stress.","authors":"Raissa Tainá Puntel, Raquel Stefanello, Cristiano Rodrigo Bohn Rhoden, Lucio Strazzabosco Dorneles","doi":"10.1080/15287394.2025.2534859","DOIUrl":"https://doi.org/10.1080/15287394.2025.2534859","url":null,"abstract":"<p><p>Cadmium (Cd) is a highly toxic heavy metal commonly introduced into agricultural soils through phosphate fertilizers. Cadmium accumulation poses serious risks to human health, crop productivity, and environmental sustainability. Although the toxic effects of Cd on plant development are well documented, effective and sustainable strategies to mitigate its impact on seed germination and early plant growth remain limited. Nanomaterials such as graphene oxide (GO) demonstrated potential in alleviating abiotic stress, but application in Cd-contaminated agricultural systems requires further investigation. Therefore, the aim of this study was to examine the potential of GO as a possible mitigator of adverse Cd-initiated effects on seed germination and initial growth of rice (<i>Oryza sativa</i> L.) seedlings. Rice seeds were treated with varying concentrations of GO (0 to 500 mg/L), Cd chloride (0 to 0.4 mM), and combination of GO + Cd chloride simultaneously. The experiment was conducted on a germitest paper, where the rolls were kept for 7 days in a germination chamber (25 ± 2 °C and 12 hr light). The germination and seedling growth were subsequently assessed. Cadmium exposure inhibited seed germination and early seedling development, particularly at concentrations from 0.1 mM. Graphene oxide, on the other hand, proved to be a suitable alternative to mitigate Cd-induced stress, showing a significant enhanced germination at concentrations of up to 250 mg/L. Graphene oxide demonstrated potential as a protective agent against Cd-induced toxicity in rice seeds, suggesting its promising application in improving crop resilience in metal-contaminated environments.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-13"},"PeriodicalIF":2.3,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root nutrient uptake, photosynthetic transients, growth and leaf anatomy of <i>Citharexylum myrianthum</i> suggest tolerance to soil cadmium toxicity.","authors":"Vitor Luís Santos Silva, Martielly Santana Dos Santos, Valéria Ferreira Fernandes, Rodrigo Barbosa Kloss, Carlos Priminho Pirovani, Pedro Antônio Oliveira Mangabeira, Marcel Giovanni Costa França","doi":"10.1080/15287394.2025.2531901","DOIUrl":"https://doi.org/10.1080/15287394.2025.2531901","url":null,"abstract":"<p><p>The increase in water and soil contamination by potentially toxic elements (PTEs) constitutes a major environmental concern. Amongst these elements, cadmium (Cd) stands out due to its high mobility in both soil and plant vascular tissue. Consequently, effective strategies for PTE removal are urgently needed. This study aimed to examine the growth, anatomical and cellular traits, chlorophyll fluorescence, macro- and micronutrients content of Pau-Viola (<i>Citharexylum myrianthum</i> Cham.) seedlings exposed to different Cd concentrations 0.25, 0.5, or 0.75 μmol/kg in soil. <i>C. myrianthum</i> seedlings exposed to Cd exhibited minimal alterations in morphophysiological attributes. Cadmium was predominantly retained in the root system with limited translocation to the shoots, thereby preserving growth and nutritional balance. The lower Cd accumulation in the leaves helped maintain the integrity of the photosynthetic electron transport chain and the chlorophyll index, resulting in only minor changes in chlorophyll <i>a</i> fluorescence transients. The observed tolerance of <i>C. myrianthum</i> to Cd-initiated toxicity suggests metallic potential use in reforestation programs for Cd-contaminated areas, as the species demonstrated resilience and physiological stability under such conditions.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Myrciaria dubia (Kunth) McVaugh</i> hydroalcoholic extract with bioactive compounds, antitumor and photoprotective activity against UVB radiation.","authors":"Mariana Tonello Biffi, Ingrithy Vendruscolo, Israel Keller Silva, Sabrina Ishikawa, Patrícia Aline Bressiani, Anna Karolina Gomes Oliveira, Virginia Marcia Concato, Wander Rogério Pavanelli, Irede Angela Lucini Dalmolin, Guilherme Bertoldo, Elisângela Düsman","doi":"10.1080/15287394.2025.2532777","DOIUrl":"https://doi.org/10.1080/15287394.2025.2532777","url":null,"abstract":"<p><p>The objective of this study was to produce and characterize a hydroalcoholic extract of <i>Myrciaria dubia (Kunth) McVaugh</i> (Camu-Camu) as well as determine antitumor and photoprotective potential. Data showed that 20 min and 50% ethanol:50% water were the best conditions for extraction in terms of phenolic compounds. The antioxidant capacity of the extract was determined utilizing the DPPH method and obtained an EC₅₀ value of 100.0 ± 3.2 μg/L. The ascorbic acid value detected was 3,504.78 ± 63.5 mg/100 ml using the Balentine method. The MTT cytotoxicity test demonstrated that the extract was not cytotoxic for renal cells of <i>Macaca mulatta</i> (LLC-MK2), but in fact initiated an increase in cell viability to 239.56%. Using the same assay with tumor cells, the extract demonstrated cytotoxic effect for human liver (HuH-7.5) and renal carcinoma (786-0) resulting in a cell viability of 22 and 20%, respectively. Ultraviolet type B radiation induced mortality of LLC-MK2 cells with cytotoxic effect proportional to the concentrations employed; however, the extract of Camu-Camu was able to promote photoprotection, especially in post-treatment with a viability of 38.79% without treatment for 178.8% in the post-treatment. Data demonstrated that the extract of Camu-Camu may be considered an important substance to prevent damage generated by ultraviolet radiation and as an anti-tumor agent.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-14"},"PeriodicalIF":2.3,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive phytochemical profiling and cytotoxic assessment of <i>Caucasalia macrophylla</i> coupled with in silico docking and miRNA (miR-19b, miR-20a, miR-126, miR-155, miR-200c)-Mediated gene regulation analysis.","authors":"Zeynep Betül Sarı, Muhammet Emin Sarı, Emine Incilay Torunoğlu, Alper Durmaz, Abidin Gümrükçüoğlu, Erdi Can Aytar","doi":"10.1080/15287394.2025.2527653","DOIUrl":"https://doi.org/10.1080/15287394.2025.2527653","url":null,"abstract":"<p><p>This study aimed to identify the chemical constituents of the methanol extract of <i>Caucasalia macrophylla</i> using GC-MS and HPLC-DAD techniques. The cytotoxic activity was determined by the MTT assay using human breast adenocarcinoma cells (MDA-MB-231) and hTERT-immortalized mammary epithelial cells (hTERT-HME1). The half-maximal inhibitory concentration (IC₅₀) for MDA-MB-231 cells was found to be 1.2 mg/ml. This concentration inhibited cell proliferation in a concentration-dependent manner. In addition, the extract was tested using a miRNA Isolation Kit to assess the mRNA expression profiles in both MDA-MB-231 and hTERT-HME1 cell lines. In cancer cells (MDA-MB-231), the expression of miR-126 was significantly upregulated 2.2-fold, while miR-155 and miR-200c were significantly downregulated 0.3-fold and 0.4-fold respectively. In healthy cells (hTERT-HME1), the expression levels of miR-19b, miR-155, and miR-200c were also significantly reduced. Further a comprehensive molecular docking analysis was conducted against phosphoinositide 3-kinase (PI3K). Among the tested compounds, rutin exhibited the strongest binding affinity with a binding energy of - 9.1 kcal/mol. This compound demonstrated potential anticancer effects by modulating PI3K activity. The findings suggest that modulation of signaling pathways by natural compounds may represent a promising approach for development of novel cancer therapies.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-18"},"PeriodicalIF":2.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipids potentially contribute to exacerbated inflammatory markers in Metabolic Syndrome mice acutely following pulmonary nanoparticle exposure.","authors":"Akshada Shinde, Li Xia, Arjun Pitchai, Jenna N Swihart, Christina Ferreira, Jonathan Shannahan","doi":"10.1080/15287394.2025.2527646","DOIUrl":"10.1080/15287394.2025.2527646","url":null,"abstract":"<p><p>Metabolic Syndrome (MetS) is a combination of disturbances including dyslipidemia. Epidemiological assessments demonstrated that individuals with MetS exhibit exacerbated inflammation following particulate inhalation. The mechanisms underlying these inflammatory responses remain to be elucidated, preventing the development of strategies to protect vulnerable and prevalent populations. Lipids dysregulated in MetS are intricately involved in inflammatory regulation. Particulate exposure-induced modifications in bioactive lipids were suggested to mediate inflammation in MetS susceptible mice. To assess this hypothesis, mice were fed a control diet or a high-fat western diet for 14-weeks to generate healthy or MetS models. Mice were exposed to silver nanoparticles (AgNPs) via oropharyngeal aspiration and acute toxicity endpoints determined 4-h post-exposure. Analysis of bronchoalveolar lavage fluid demonstrated that the AgNP exposure produced neutrophilia in both healthy and MetS mouse models, which was exacerbated in MetS mice. Gene expression of chemokine ligand-1 and macrophage inflammatory protein-2 were upregulated equally in both healthy and MetS mice. Pulmonary lipids were measured utilizing an MRM profiling approach, which noted induction of pro-inflammatory lipids in MetS following AgNP exposure compared to healthy mice. Specifically, arachidonic acid, prostaglandin-E2, prostaglandin-D2, 12-hydroxyeicosatetraenoic acid, and leukotriene-B4 were elevated in MetS mice following exposure, supporting the contribution of lipids to exacerbated inflammation. Further, pulmonary gene expression demonstrated upregulation of lipid metabolism enzymes arachidonate 15-lipoxygenase and prostaglandin-endoperoxide synthase 2 in MetS mice following AgNP exposure. Overall, data suggest dysregulation of pro-inflammatory lipid mediators contributes to early exacerbations in inflammatory responses observed in MetS animals following particulate exposures that might be targeted for therapeutic interventions.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-19"},"PeriodicalIF":2.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12282671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutagenic assessment and toxicological impact of bergenin in a phenolic-enriched extract from <i>Endopleura uchi</i> (Huber) Cuatrec bark, a medicinal plant from the Amazon rainforest.","authors":"Elkejer Ribeiro da Cruz, Dione Silva Corrêa, Jéssica Machado Miri, Juliana Bondan da Silva, Jayne Torres de Sousa, Ingrid Vicente Farias, Flávio Henrique Reginatto, Juliana da Silva, Ivana Grivicich, Alexandre de Barros Falcão Ferraz, Jaqueline Nascimento Picada","doi":"10.1080/15287394.2025.2464920","DOIUrl":"10.1080/15287394.2025.2464920","url":null,"abstract":"<p><p><i>Endopleura uchi</i> bark traditionally used in folk medicine attributed to its anti-inflammatory properties is due to the presence of bergenin. This study aimed to determine the toxicological parameters associated with exposure to a phenolic-enriched extract of <i>E. uchi</i> bark and bergenin a bioactive byproduct of this compound. The total phenolic and flavonoid contents were determined through spectrometric analyses, while phenolic compounds were identified using ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-MS). Antioxidant activity was assessed in vitro using the DPPH assay. Cytotoxicity and genotoxicity were assessed via the MTT assay and comet assay, respectively, whereas mutagenic activity was examined using Salmonella/microsome assay and micronucleus (MN) test. A high content of phenolic (732.22 ± 9.48 mg/g) GAE (gallic acid equivalence) and flavonoid 252.47 ± 5.7 mg/g QE (quercetin) compounds was found in bergenin the phenolic-enriched extract byproduct as well as isomers of gallic acid, epicatechin, isoquercitrin, castalagin, punicalin, and punicalagin. The DPPH value was 23.74 ± 0.45 μg/ml. In MTT assay, the extract exhibited an IC50 of 72.5 ± 2.6 µg/ml. Both extract and bergenin displayed genotoxic activity in L929 fibroblast cells at 50 µg/ml but not mutagenic effects in the Salmonella/microsome assay or MN test. Despite the genotoxic actions, <i>E. uchi</i> bark and bergenin extract did not induce gene or chromosomal mutations, suggesting a low risk of compromising genomic stability. The presence of bioactive compounds such as bergenin and punicalagin in <i>E. uchi</i> bark demonstrates a therapeutic potential of this native tree for treating inflammatory diseases.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"495-504"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-response effect of polyphenon-60 from green tea (P60-GT) on hexavalent chromium-induced genotoxic damage and apoptosis in mice.","authors":"Lourdes Montserrat Hernández-Cortés, Víctor Manuel Mendoza-Núñez, Alda Rocío Ortiz-Muñiz, María Del Carmen García-Rodríguez","doi":"10.1080/15287394.2025.2455956","DOIUrl":"10.1080/15287394.2025.2455956","url":null,"abstract":"<p><p>This study aimed to examine the dose-response effects of polyphenon-60 derived from green tea (P60-GT) on hexavalent chromium [Cr(VI)]-induced genotoxic damage and apoptosis. Male Hsd:ICR mice were divided into 4 groups: (1) Control (vehicle only), (2) P60-GT (15, 30, or 45 mg/kg gavage), (3) Cr(VI) (20 mg/kg of CrO₃ intraperitoneally), and (4) P60-GT+CrO₃ (P60-GT administered 4 hr before CrO₃). Peripheral blood samples were collected at 24, 48, and 72 hr to assess the number of micronuclei (MN), apoptosis, and cell viability, while plasma 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels were measured at 0 and 48 hr. Cr(VI) significantly increased MN frequency, suppressed 8-OHdG repair, and reduced cell viability. Pre-treatment with P60-GT reduced MN frequency by up to 74%, with the 30 mg/kg dose demonstrating the highest efficacy. This dose restored cell viability, enhanced 8-OHdG repair, and enhanced apoptosis, suggesting activation of DNA repair and apoptotic pathways as potential antigenotoxic mechanisms. The 15 mg/kg dose exhibited anti-apoptotic effects, while the 30 and 45 mg/kg doses promoted apoptosis. However, the 45 mg/kg dose resulted in 100% lethality by 72 hr, likely due to synergistic toxicity with Cr(VI). These findings demonstrate the dose-dependent protective effects of P60-GT and emphasize the need for dosage optimization to maximize therapeutic benefits while minimizing toxicity.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"479-494"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytotoxicity and cytogenotoxic effects of extracts from the medicinal bark of <i>Rhamnus purshiana</i> DC. (Rhamnaceae).","authors":"Kimberly Silva Souza, Antonio Rodrigues da Cunha Neto, Daniela Aparecida Chagas-Paula, Sandro Barbosa, Geraldo Alves da Silva, João Vitor Barbosa Calvelli","doi":"10.1080/15287394.2025.2466665","DOIUrl":"10.1080/15287394.2025.2466665","url":null,"abstract":"<p><p>Natural products, although frequently associated with beneficial effects and considered harmless, still require thorough analysis; therefore, their bioactive compounds need to be used with caution. Biological assays using plant models represent an appropriate alternative for evaluating the cytogenotoxicity associated with these products. In this study, the phyto- cytotoxic potential of extracts from the bark of <i>Rhamnus purshiana</i> DC. a species widely used for treatment of constipation was investigated due to its anthraquinone content. To this end, seeds of <i>Lactuca sativa</i> L. were employed in phyto-cytogenotoxic assays under chronic exposure to lyophilized and spray-dried extracts. The results showed that extraction methods directly influence the phytochemical composition and biological effects of the extracts. Both extracts exhibited high anthraquinone content, expressed as cascaroside A, with concentrations ranging from 45.65 to 72.17 μg/ml. The spray-dried extract demonstrated a more potent inhibitory effect on morphological parameters, such as root elongation, while lyophilized extract exhibited higher cytotoxicity. Both extracts induced mitodepressive effects and aneugenic damage. Notably, the lyophilized extract induced a 300% increase in mitotic abnormalities compared to control, with a higher frequency of C-metaphases and stickiness. Although <i>R. purshiana</i> is widely used in traditional medicine, the scarcity of studies on cascaroside A raises concerns regarding socio-environmental safety and continued use of this species in dietary supplements.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"505-517"},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> immunotoxic evaluation of herbicides in RAW 264.7 cells.","authors":"Larissa Vivan Cestonaro, Aline Mocellin Conte, Fernanda Capitanio Goldoni, Nara Lins Meira Quintão, Solange Cristina Garcia, José Roberto Santin, Marcelo Dutra Arbo","doi":"10.1080/15287394.2025.2450418","DOIUrl":"10.1080/15287394.2025.2450418","url":null,"abstract":"<p><p>Weeds are a concern in agriculture and the use of herbicides constitutes an effective, efficient, and economical way to control their growth. Recent discoveries of herbicides are promising for the management of resistant weeds. However, there is a gap in the knowledge of the toxic effects of some herbicides previously reported on immune cells. The present study aimed to examine cellular immunotoxicity of three herbicides (clomazone, glyphosate, and sulfentrazone) after 96 hr incubation utilizing RAW 264.7 BALB/c mouse monocyte/macrophage-like cell line to elucidate the role of some toxicological pathways. Data demonstrated the herbicides clomazone, glyphosate, and sulfentrazone initiated a cytotoxic effect as evidenced by EC₅₀ values of 429.2; 53.7; 866.6 mg/L, respectively. Clomazone and sulfentrazone, at all concentrations, induced excess production of reactive oxygen (ROS) and reactive nitrogen (RNS) free radicals. An immunosuppression was observed in RAW 264.7 cells after incubation with 50 or 100 mg/L glyphosate and 500 or 1000 mg/L sulfentrazone. In addition, all herbicides produced mitochondrial depolarization and decreased tumor necrosis factor-α (TNF-α) levels. This constitutes the first report of the effects of clomazone and sulfentrazone on RAW 264.7 cells, including reduced TNF-α levels, indicating the adverse influence of herbicides on the immune system.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"433-446"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, <i>in vitro</i> and <i>in silico</i> activity of the methanolic extract derived from <i>Vassobia breviflora</i> against clinically relevant bacteria.","authors":"Altevir Rossato Viana, Erdi Can Aytar, Nickolas Pippi, Daniel Santos, Cristiano Rodrigo Bohn Rhoden, Bruno Stefanello Vizzotto, Erico Marlon Moraes Flores, André Passaglia Schuch, Luciana Maria Fontanari Krause","doi":"10.1080/15287394.2025.2453858","DOIUrl":"10.1080/15287394.2025.2453858","url":null,"abstract":"<p><p>This study aimed to identify chemical compounds derived from Vassobia breviflora methanolic extract using ESI-ToF-MS and their antioxidant potential activity utilizing the following methods: total phenols, DPPH, and ABTS^•+. The MTT assay measured cytotoxic activity, while DCFH-DA and nitric oxide assays were employed to determine reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels using African green monkey kidney (VERO) and human keratinocyte (HaCat) cell lines. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assessed in seven clinical isolates and nine ATCC strains. Biofilm inhibition was tested against four biofilm-forming strains. The antioxidant properties of the methanolic extract were identified as follows: 35.74 mg GAE/g (gallic acid equivalents)/g for total phenols, 10.5 µg/ml for DPPH, and 50.68 µmol trolox/µg for ABTS^•+. The mean inhibitory concentration (IC₅₀) values were 622.86 µg/ml (VERO) and 784.33 µg/ml (HaCat). These concentrations did not markedly alter levels of ROS and RNS. Conversely, <i>Bacillus cereus</i> β-hemolytic displayed higher sensitivity to the extract, with MIC of 64 µg/ml and MBC of 128 µg/ml. <i>Enterococcus faecium</i> exhibited the lowest biofilm formation among the tested bacteria. The studied plant exhibited activity against all bacterial strains at concentrations lower than the IC50 VERO and HaCat cells, suggesting potential for future studies. Data present a comprehensive molecular docking analysis against the HlyIIR protein (PDB ID: 2FX0) and determined antimicrobial and endocrine-modulating potentials. Notably, lancifodilactone I and nicandrin B demonstrated the strongest binding affinities, with binding energies of -9.8 kcal/mol and -8.3 kcal/mol, respectively, and demonstrated significant antimicrobial effects against B. cereus. In addition, several compounds showed potential interactions with nuclear receptors, indicating potential endocrine-modulating effects. These findings provide insights into developing target-specific antimicrobial therapies and endocrine-modulating agents.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"463-478"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}