Phytotoxicity and cytogenotoxic effects of extracts from the medicinal bark of Rhamnus purshiana DC. (Rhamnaceae).

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES
Kimberly Silva Souza, Antonio Rodrigues da Cunha Neto, Daniela Aparecida Chagas-Paula, Sandro Barbosa, Geraldo Alves da Silva, João Vitor Barbosa Calvelli
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引用次数: 0

Abstract

Natural products, although frequently associated with beneficial effects and considered harmless, still require thorough analysis; therefore, their bioactive compounds need to be used with caution. Biological assays using plant models represent an appropriate alternative for evaluating the cytogenotoxicity associated with these products. In this study, the phyto- cytotoxic potential of extracts from the bark of Rhamnus purshiana DC. a species widely used for treatment of constipation was investigated due to its anthraquinone content. To this end, seeds of Lactuca sativa L. were employed in phyto-cytogenotoxic assays under chronic exposure to lyophilized and spray-dried extracts. The results showed that extraction methods directly influence the phytochemical composition and biological effects of the extracts. Both extracts exhibited high anthraquinone content, expressed as cascaroside A, with concentrations ranging from 45.65 to 72.17 μg/ml. The spray-dried extract demonstrated a more potent inhibitory effect on morphological parameters, such as root elongation, while lyophilized extract exhibited higher cytotoxicity. Both extracts induced mitodepressive effects and aneugenic damage. Notably, the lyophilized extract induced a 300% increase in mitotic abnormalities compared to control, with a higher frequency of C-metaphases and stickiness. Although R. purshiana is widely used in traditional medicine, the scarcity of studies on cascaroside A raises concerns regarding socio-environmental safety and continued use of this species in dietary supplements.

鼠李药用树皮提取物的植物毒性和细胞基因毒性作用。(鼠李科)。
天然产品虽然经常与有益作用联系在一起并被认为是无害的,但仍需要进行彻底的分析;因此,它们的生物活性成分需要谨慎使用。使用植物模型的生物测定是评估与这些产品相关的细胞遗传毒性的适当替代方法。本研究对鼠李树皮提取物的植物细胞毒潜能进行了研究。研究了一种因蒽醌含量高而被广泛用于治疗便秘的植物。为此,在冻干和喷雾干燥提取物的长期暴露下,研究了乳酸种子的植物细胞基因毒性。结果表明,提取方法直接影响到提取物的植物化学成分和生物效应。两种提取物均表现出较高的蒽醌含量,其浓度范围为45.65 ~ 72.17 μg/ml。喷雾干燥提取物对根伸长等形态参数具有更强的抑制作用,而冻干提取物表现出更高的细胞毒性。两种提取物均诱导线粒体抑制作用和非优生损伤。值得注意的是,与对照组相比,冻干提取物诱导的有丝分裂异常增加了300%,c -中期和黏性的频率更高。尽管红毛豆在传统医学中被广泛使用,但对cascarcar苷A的研究的缺乏引起了人们对社会环境安全性和该物种在膳食补充剂中的持续使用的担忧。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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