Evaluation of safety and efficacy of Brazilian brown propolis from Araucaria sp. in preventing colon cancer.

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES
Karoline Soares de Freitas, Iara Silva Squarisi, Letícia Teixeira Marcos de Souza, Saulo Duarte Ozelin, Lucas Teixeira de Souza Oliveira, Victor Pena Ribeiro, Jairo Kenupp Bastos, Denise Crispim Tavares
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引用次数: 0

Abstract

Brazilian propolis produced by honeybees have been widely studied, but few data exist regarding the safety and pharmacological potential of this natural product. The aim of the present study was to examine the toxicity, genotoxicity, and chemoprevention effects attributed to exposure to the brown propolis hydroalcoholic extract (BPHE) of Araucaria sp. Acute oral toxicity test was conducted using Wistar Hannover rats, demonstrating that the highest dose tested (2,000 mg/kg b.w.) produced no apparent adverse effects or lethality. The micronucleus (MN) genotoxicity test was conducted using peripheral blood from Swiss mice, which also noted that BPHE did not induce significant chromosomal damage. It is of interest that BPHE at a dose of 12 mg/kg b.w. exhibited antigenotoxic effects against the doxorubicin (DXR)-induced damage. However, BPHE did not influence the depletion of reduced glutathione induced by DXR in mice. It is noteworthy that BPHE exerted chemopreventive effects at doses 6, 12, and 24 mg/kg b.w. The determination of this effect of BPHE on colon carcinogenesis was examined using aberrant crypt foci (ACF) as evidenced by histological analysis. The colons of animals treated with BPHE (12 mg/kg b.w.) exhibited a significant reduction in staining for proliferating cell nuclear antigen (PCNA) and cyclooxygenase-2 (COX-2) protein following 1,2-dimethylhydrazine (DMH)-and BPHE combined treatments. Hence, it is conceivable that the anti-inflammatory activity of the chemical constituents of BPHE are involved in its chemopreventive action against colon carcinogenesis as evidenced from ACF assay. Therefore, BHPE was found to be a safe product, without any apparent significant acute adverse risk. Further, the extract exhibited antigenotoxic and anticarcinogenic activities which may be considered for beneficial uses in colon carcinogenesis.

巴西棕蜂胶预防结肠癌的安全性和有效性评价。
由蜜蜂生产的巴西蜂胶已被广泛研究,但关于这种天然产品的安全性和药理潜力的数据很少。本研究的目的是研究暴露于褐蜂胶水醇提取物(BPHE)的毒性、遗传毒性和化学预防作用。用威斯达汉诺威大鼠进行了急性口服毒性试验,表明最高剂量(2,000 mg/kg b.w)没有产生明显的不良反应或致死。使用瑞士小鼠外周血进行微核(MN)遗传毒性试验,也注意到BPHE没有引起显着的染色体损伤。令人感兴趣的是,在剂量为12 mg/kg b.w.时,BPHE对阿霉素(DXR)诱导的损伤表现出抗原毒性作用。然而,BPHE不影响DXR诱导小鼠还原性谷胱甘肽的消耗。值得注意的是,BPHE在剂量为6、12和24 mg/kg b.w时发挥了化学预防作用。通过组织学分析,利用异常隐窝灶(ACF)检测了BPHE对结肠癌发生的影响。在1,2-二甲基肼(DMH)和BPHE联合处理后,BPHE (12 mg/kg b.w)处理的动物结肠增殖细胞核抗原(PCNA)和环氧化酶-2 (COX-2)蛋白染色显著降低。因此,可以想象BPHE化学成分的抗炎活性参与了其抗结肠癌的化学预防作用,正如ACF测定所证明的那样。因此,BHPE被认为是一种安全的产品,没有任何明显的显著急性不良风险。此外,该提取物显示出抗毒性和抗癌活性,这可能被认为在结肠癌发生中有有益的用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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