Mass spectrometry (MS)-based metabolomics of plasma and urine in dry eye disease (DED)-induced rat model.

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES
Hyang Yeon Kim, Jung Dae Lee, HongYoon Kim, YuJin Kim, Jin Ju Park, Soo Bean Oh, Hyeyoon Goo, Kyong Jin Cho, Kyu-Bong Kim
{"title":"Mass spectrometry (MS)-based metabolomics of plasma and urine in dry eye disease (DED)-induced rat model.","authors":"Hyang Yeon Kim, Jung Dae Lee, HongYoon Kim, YuJin Kim, Jin Ju Park, Soo Bean Oh, Hyeyoon Goo, Kyong Jin Cho, Kyu-Bong Kim","doi":"10.1080/15287394.2024.2393770","DOIUrl":null,"url":null,"abstract":"<p><p>Dry eye disease (DED) is an ophthalmic disease associated with poor quality and quantity of tears, and the number of patients is steadily increasing. The aim of this study was to determine plasma and urine metabolites obtained from DED scopolamine animal model where dry eye conditions (DRY) are induced. It was also of interest to examine whether DED (scopolamine) rat model was exacerbated by treatment with benzalkonium chloride (BAC). Subsequently, plasma and urine metabolites were analyzed using liquid chromatography (LC) and gas chromatography (GC)-mass spectrometry (MS), respectively. Data demonstrated that DED indicators such as tear volume, tear breakup time (TBUT), and corneal damage in the DED groups (DRY and BAC group) differed from those of control (CON). Similar results were noted in inflammatory factors such as interleukin (IL-1β), IL-6, and tumor necrosis factor (TNF)-α. In the partial least squares-discriminant analysis (PLS-DA) score plots, the three groups were distinctly separated from each other. In addition, the related metabolites were also associated with these distinct separations as evidenced by 9 and 14 in plasma and urine, respectively. Almost all of the selected metabolites were decreased in the DRY group compared to CON, and the BAC group was lower than the DRY. In plasma and urine, lysophosphatidylcholine/lysophosphatidylethanolamine, organic acids, amino acids, and sugars varied between three groups, and these metabolites were related to inflammation and oxidative stress. Data suggest that treatment with scopolamine with/without BAC-induced DED and affected the level of systemic metabolites involved in inflammation and oxidative stress.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-14"},"PeriodicalIF":2.3000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Toxicology and Environmental Health-Part A-Current Issues","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/15287394.2024.2393770","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Dry eye disease (DED) is an ophthalmic disease associated with poor quality and quantity of tears, and the number of patients is steadily increasing. The aim of this study was to determine plasma and urine metabolites obtained from DED scopolamine animal model where dry eye conditions (DRY) are induced. It was also of interest to examine whether DED (scopolamine) rat model was exacerbated by treatment with benzalkonium chloride (BAC). Subsequently, plasma and urine metabolites were analyzed using liquid chromatography (LC) and gas chromatography (GC)-mass spectrometry (MS), respectively. Data demonstrated that DED indicators such as tear volume, tear breakup time (TBUT), and corneal damage in the DED groups (DRY and BAC group) differed from those of control (CON). Similar results were noted in inflammatory factors such as interleukin (IL-1β), IL-6, and tumor necrosis factor (TNF)-α. In the partial least squares-discriminant analysis (PLS-DA) score plots, the three groups were distinctly separated from each other. In addition, the related metabolites were also associated with these distinct separations as evidenced by 9 and 14 in plasma and urine, respectively. Almost all of the selected metabolites were decreased in the DRY group compared to CON, and the BAC group was lower than the DRY. In plasma and urine, lysophosphatidylcholine/lysophosphatidylethanolamine, organic acids, amino acids, and sugars varied between three groups, and these metabolites were related to inflammation and oxidative stress. Data suggest that treatment with scopolamine with/without BAC-induced DED and affected the level of systemic metabolites involved in inflammation and oxidative stress.

基于质谱(MS)技术的干眼病(DED)诱导大鼠模型血浆和尿液代谢组学研究。
干眼症(DED)是一种与泪液质量和数量低下有关的眼科疾病,患者人数正在稳步增加。本研究旨在确定从诱导干眼症(DRY)的 DED 东莨菪碱动物模型中获得的血浆和尿液代谢物。此外,研究人员还想了解 DED(东莨菪碱)大鼠模型是否会因使用苯扎氯铵(BAC)治疗而恶化。随后,分别使用液相色谱法(LC)和气相色谱-质谱法(MS)对血浆和尿液中的代谢物进行了分析。数据显示,DED 组(DRY 和 BAC 组)的泪液量、泪液破裂时间(TBUT)和角膜损伤等 DED 指标与对照组(CON)不同。白细胞介素(IL-1β)、IL-6和肿瘤坏死因子(TNF)-α等炎症因子也有类似结果。在偏最小二乘判别分析(PLS-DA)得分图中,三组之间有明显的区分。此外,血浆和尿液中的 9 种和 14 种相关代谢物也与这些明显的分离有关。与对照组相比,DRY 组几乎所有选定的代谢物都有所下降,而 BAC 组则低于 DRY 组。血浆和尿液中的溶血磷脂酰胆碱/溶血磷脂酰乙醇胺、有机酸、氨基酸和糖在三组之间存在差异,这些代谢物与炎症和氧化应激有关。数据表明,东莨菪碱加/不加BAC治疗诱发DED,并影响全身代谢物水平,这些代谢物与炎症和氧化应激有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信