New Microbiologica最新文献_第4页

Epidemiology and molecular characterization of respiratory viruses at the end of COVID-19 pandemic in Lombardy, Northern Italy. 意大利北部伦巴第 COVID-19 大流行结束时呼吸道病毒的流行病学和分子特征。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Leonardo Sclavi, Marta Bertelli, Serena Messali, Arnaldo Caruso, Francesca Caccuri

{"title":"Epidemiology and molecular characterization of respiratory viruses at the end of COVID-19 pandemic in Lombardy, Northern Italy.","authors":"Leonardo Sclavi, Marta Bertelli, Serena Messali, Arnaldo Caruso, Francesca Caccuri","doi":"","DOIUrl":"","url":null,"abstract":"The COVID-19 pandemic forced the adoption of non-pharmaceutical interventions (NPIs) which influenced the circulation of other respiratory pathogens, such as Influenza virus (FLU), Parainfluenza virus (PIV), Respiratory Syncytial virus (RSV), Rhinovirus (RV), Enterovirus (EV), Adenovirus (AdV), Human Metapneumovirus (hMPV), and Human Coronavirus (CoV). The aim of the current study was to investigate how, with the end of the pandemic, the withdrawal of the NPIs impacted on the circulation and distribution of common respiratory viruses. The analyzed samples were collected from June 2021 to March 2023 (post-pandemic period) and compared to ones from the pandemic period. Nucleic acid detection of all respiratory viruses was performed by multiplex real time Polymerase Chain Reaction (PCR) and sequencing was conducted by Next Generation Sequencing (NGS) technique. Our analysis shows that the NPIs adopted against SARS-CoV-2 were also effective in controlling the spread of other respiratory viruses. Moreover, we documented how RV/EVs were the most commonly identified species, with the more abundant strains represented by Coxsackievirus (CV)-A/B and RV-A/C. RV/EVs were also detected in some co-infection cases; in particular, the majority of co-infections concerned CV-B/RV-A, CV-B/ECHO. Given the pandemic potential of respiratory viruses, accurate molecular screening is essential for a proper surveillance and prevention strategy.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"80-87"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lamivudine plus dolutegravir as a switch strategy in children: three case reports. 拉米夫定加多鲁曲韦作为儿童的转换策略：三份病例报告。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Laura Labate, Claudia Bartalucci, Lucia Taramasso, Giorgia Brucci, Antonio Vena, Matteo Bassetti, Antonio Di Biagio

{"title":"Lamivudine plus dolutegravir as a switch strategy in children: three case reports.","authors":"Laura Labate, Claudia Bartalucci, Lucia Taramasso, Giorgia Brucci, Antonio Vena, Matteo Bassetti, Antonio Di Biagio","doi":"","DOIUrl":"","url":null,"abstract":"Lamivudine (3TC)/dolutegravir (DTG) single tablet regimen (STR) has shown long-term efficacy and tolerability in people living with HIV (PLWH). Dolutegravir has been approved for use in children, while data on the efficacy of 3TC plus DTG in maintaining virological suppression in this population are still under evaluation. In this case series, we describe three children with perinatally acquired HIV who maintained virological suppression after switching antiretroviral therapy to DTG/3TC. We present three case reports of three children enrolled in the Italian Register for HIV Infection in Children: a 9-year-old boy, a 10-year-old girl, and a 2-year-old girl with perinatally acquired HIV who immediately started antiretroviral therapy with a three-drug regimen upon diagnosis, which occurred at delivery, after 6 months of life, and after 2 years of life, respectively. They achieved and maintain virological suppression after 1, 6, and 7 months of therapy, respectively; then a switch strategy was performed with a two-drug regimen with DTG/3TC STR at the age of 7 years for the first child and at the age of 9 years for the second, while the third was switched to a DTG plus 3TC not STR, owing to weight requirements, at the age of 2 years and 10 months. All children maintained virological suppression at last follow-up visit (January 2024), showing an excellent growth curve and maintaining good adherence and tolerability to DTG plus 3TC. A two-drug regimen with DTG/3TC demonstrated efficacy in maintaining virological suppression in a switch strategy in these children, with important advantages such as better tolerability and comfort of taking a single tablet once daily.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"111-115"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Risk Human Papilloma Virus Genotype Distribution and Correlation with Cervical Cytomorphological Data in Turkish and Immigrant Women in Mersin Province. 梅尔辛省土耳其妇女和移民妇女的高危人类乳头瘤病毒基因型分布及其与宫颈细胞形态学数据的相关性

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Murat Yaman, Hacer Ece Arslan Özcan, Ayfer Bakir

{"title":"High-Risk Human Papilloma Virus Genotype Distribution and Correlation with Cervical Cytomorphological Data in Turkish and Immigrant Women in Mersin Province.","authors":"Murat Yaman, Hacer Ece Arslan Özcan, Ayfer Bakir","doi":"","DOIUrl":"","url":null,"abstract":"Human papilloma virus (HPV) is the most common sexually transmitted viral agent in the world and the most common cause of cervical cancer. HPV prevalence and genotype distribution vary by region and demographic data. In a province in the south of Turkey that constantly receives immigration, we aimed to determine the prevalence of high-risk HPV (HR-HPV) genotypes, evaluate the compatibility between cervical Pap smear cytology results patients and HR-HPVs, and make an up-to-date contribution to the elucidation of epidemiological data. In this single-centre study, a total of 12,641 women aged 18 and over were evaluated retrospectively from January 2019 to July 2022. HPV detection and genotyping were analysed by the PCR method. Bethesda scoring was used for Pap smear cytological evaluation. The overall prevalence of HR-HPV was 12.6% (12.7% in Turkish women, 11.2% in foreign women). Among the typed HPVs that were detected, HPV-16 (31%) was found first, followed by HPV-18 (8%). The prevalence of HR-HPV was higher in women with abnormal cytology (977/1762, 55.4%) than in women with normal cytology (620/10879, 5.7%) (p<0.001). Turkey doesn't yet have a national HPV immunisation program. We think that determining the specific regional frequency of other HR-HPVs separately will be useful in the follow-up of the natural course of the type-specific infection and in vaccine studies in the future.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"88-97"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long COVID: lights and shadows on the clinical characterization of this emerging pathology. 长COVID：这一新兴病理临床特征的光与影。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Viola Cogliandro, Paolo Bonfanti

引用次数: 0

In vitro analysis of Porcine Endogenous Retroviruses in different pig cell types. 猪内源性逆转录病毒在不同猪细胞类型中的体外分析。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Andrea Cacciamali, Silvia Dotti, Riccardo Villa

{"title":"In vitro analysis of Porcine Endogenous Retroviruses in different pig cell types.","authors":"Andrea Cacciamali, Silvia Dotti, Riccardo Villa","doi":"","DOIUrl":"","url":null,"abstract":"The shortage of organs for human transplantation is a topic of extreme interest, and xenotransplantation with porcine organs has been recognized as a promising solution. However, the potential spillover linked to infectious agents present in pigs remains a concern. Among these, Pig Endogenous Retroviruses (PERVs), whose proviral DNAs are integrated in the genome of all pig breeds, represent an extremely important biological risk. This study aims to evaluate PERVs distribution in several swine cell lines and samples of domestic and feral pigs. Moreover, the capacity of PERVs to infect human and non-human primate cells and to integrate in the cellular genome was tested by Real-Time PCR and by Reverse Transcriptase assay. Results indicated a widespread diffusion of PERVs both in cell lines and samples analysed: the viral genome was found in all the established cell lines, in 40% of the primary cell lines and in 60% of the tissue samples tested. The assays indicated that the virus can be transmitted from porcine to human cells: in the specific case, infected NSK and NPTr cells allow passage to human 293 and MRC-5 cells with active production of the virus demonstrable via PCR and RT assay. In light of these aspects and also the lack of studies on PERVs, it appears clear that there are still many questions to be clarified, also by means of future studies, before xenotransplantation can be considered microbiologically safe.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"38-46"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of cases with co-infection of COVID-19 and pulmonary aspergillosis. 对同时感染 COVID-19 和肺曲霉菌病的病例进行分析。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Hui Zheng Hu, Li Bo Wu, Qian Liu, Bin Yan Wu, Yu Wang, Rui Ying Wang

引用次数: 0

Evaluation of the Pneumonia Panel on laboratory samples from an Italian pediatric center: results from a monocentric study. 对意大利一家儿科中心实验室样本中的肺炎样本进行评估：单中心研究的结果。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Cristiana de Luca, Emanuela Gallo, Gabriella Tripepi, Ornella Leone

引用次数: 0

Tenofovir disoproxil fumarate in the treatment of COVID-19: Evaluation of 78 patients. 富马酸替诺福韦酯治疗 COVID-19：对 78 名患者的评估。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Gamze Şanlıdağ İşbilen, Isabel Raika Durusoy Onmuş, Ilkin Çankayalı, Kubilay Demirağ, Mehmet Uyar, Candan Çiçek, Tansu Yamazhan, Hüsnü Pullukçu, Oğuz Reşat Sipahi

{"title":"Tenofovir disoproxil fumarate in the treatment of COVID-19: Evaluation of 78 patients.","authors":"Gamze Şanlıdağ İşbilen, Isabel Raika Durusoy Onmuş, Ilkin Çankayalı, Kubilay Demirağ, Mehmet Uyar, Candan Çiçek, Tansu Yamazhan, Hüsnü Pullukçu, Oğuz Reşat Sipahi","doi":"","DOIUrl":"","url":null,"abstract":"One of the drugs that has been suggested for the treatment of SARS-CoV-2 infection is tenofovir disoproxil (TDF). Herein, it was aimed to evaluate the outcomes of TDF receiving COVID-19 cases in terms of day 7-10 PCR negativity and day 30 survival. Patients who received TDF due to PCR-confirmed COVID-19 between 27.04.2021 and 31.12.2021 were included in our study. The primary outcome was considered to be 7-10 days of PCR negativity, while the secondary outcome was considered 30-day survival after diagnosis of COVID-19. Patients who died before completing the treatment period (7-10 days) were also considered as PCR failures. Data were analyzed both in terms of intention to treat basis and in the subgroup that survived to the end of treatment. A total of 78 patients (30 women, mean age: 61.15±18.5 years) met the inclusion criteria. In the intention to treat analysis group, one-month-mortality was 44.87% (35/78) in the overall cohort. In the end of treatment analysis group, one-month-mortality was 29.5% (18/61) in the overall cohort. Day 7-10 PCR negativity was detected in 55.7% of the overall EOT cohort. Our data suggest that TDF may be an alternative salvage treatment option in antiviral unresponsive patients. We suggest evaluating TDF in well-designed controlled trials involving treatment-naïve cases.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"47-51"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The presence of Trichomonas vaginalis in urogenital samples can affect the sensitivity of Mycoplasma hominis identification techniques, leading to an underestimation of bacterial infections. 泌尿系统样本中阴道毛滴虫的存在会影响人型支原体鉴定技术的灵敏度，导致低估细菌感染。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Valentina Margarita, Gavino Carboni, Pier Luigi Fiori, Paola Rappelli

引用次数: 0

Epidemiology and Clinical impact of single and multi-viral respiratory infections in post-pandemic era. 后流行病时代单一和多种病毒呼吸道感染的流行病学和临床影响。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Maria Vittoria Mauro, Sonia Greco, Maura Pellegrini, Tommaso Campagna, Federica Caprino, Nadia Elia, Antonio Mastroianni, Francesca Greco

{"title":"Epidemiology and Clinical impact of single and multi-viral respiratory infections in post-pandemic era.","authors":"Maria Vittoria Mauro, Sonia Greco, Maura Pellegrini, Tommaso Campagna, Federica Caprino, Nadia Elia, Antonio Mastroianni, Francesca Greco","doi":"","DOIUrl":"","url":null,"abstract":"Acute respiratory tract infections (ARI) are common diseases in children and adults and could cause severe infections in high-risk patients, like the immunocompromised and elderly, and are the leading cause of morbidity, hospitalization and mortality. This study aimed to explore the prevalence of respiratory viruses and the clinical impact of single- and multi-infection among hospitalized patients in various age groups. 3578 nasopharyngeal swabs (NPS) were analyzed for pathogen detection of acute respiratory tract infections. 930 out of 3578 NPS were diagnosed positive for at least one respiratory virus. The distribution of viral infections, prevalence and pathogen, differed significantly among age groups. Most RTI are observed in the age group over 65 years (50.6%) with a high SARS-CoV2 prevalence, following by group <5 years (25.6%), where the most frequently detected viruses were RSV, Rhinovirus, FluA-H3, MPV, and AdV. The co-infection rate also varies according to age and, in some cases, especially in older adults, could have severe clinical impact. This study emphasizes that it is important to know and analyze, in all age groups of hospitalized patients, the epidemiology of respiratory viruses, the prevalence of coinfections, and the clinical impact of various pathogens. Furthermore, in a clinical setting, the rapid diagnosis of respiratory infections by means of molecular tests is crucial not only to avoid hospital outbreaks, but also to allow early and optimal treatment to reduce morbidity and mortality.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"28-32"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0