New Microbiologica最新文献_第3页

Non-O1 and non-O139 Vibrio cholerae Septicemia and Cellulitis: a Case Report. 非 O1 和非 O139 霍乱弧菌败血症和蜂窝组织炎：病例报告。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Marcella Negri, Floriana Gona, Chiara Tassan Din, Angelo Roberto Raccagni, Costanza Bertoni, Matteo Moro, Laura Villa, Massimo Locatelli, Antonella Castagna, Monica Guffanti

{"title":"Non-O1 and non-O139 Vibrio cholerae Septicemia and Cellulitis: a Case Report.","authors":"Marcella Negri, Floriana Gona, Chiara Tassan Din, Angelo Roberto Raccagni, Costanza Bertoni, Matteo Moro, Laura Villa, Massimo Locatelli, Antonella Castagna, Monica Guffanti","doi":"","DOIUrl":"","url":null,"abstract":"Non-O1 and non-O139 Vibrio cholerae (NOVC) are serogroups that do not produce cholera toxin and are not responsible for epidemics. Even though rarely encountered in clinical practice, they can cause a spectrum of different conditions ranging from mild gastrointestinal syndrome to extraintestinal diseases, of which bacteremia and wound infections are the most severe. Risk factors for severe disease are cirrhosis, neoplasms, and diabetes mellitus. The mortality rate of NOVC bacteremia in hospitalized patients ranges from 24 to 61.5%. Incidence of NOVC infections is still rare, and consensus recommendations on treatment are not available. We report a case of NOVC bacteremia associated with severe cellulitis in an immunocompetent 75-year-old man who had eaten raw seafood in a location by the northern Adriatic Sea (Italy). Twenty-four hours after intake, he developed a high fever and vomiting. Afterwards, he started noticing the appearance of cellulitis in his right leg, which worsened in a matter of hours. The patient had a history of compensated type 2 diabetes mellitus. NOVC was isolated from both blood cultures and the leg ulcer. The non-O1, non-O139 serogroup was confirmed, and the detection of the cholera toxin gene was negative. Both tests were performed by the Reference National Laboratory of Istituto Superiore di Sanità (ISS). Multiple antimicrobial regimens were administered, with complete recovery. In conclusion, considering the severity of NOVC-associated manifestations, it is of pivotal importance to reach etiological diagnosis for a target antimicrobial therapy and to consider V. cholerae infection in the differential diagnosis in the presence of risk factors and potential exposure.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"190-193"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatitis C Virus (HCV) genotypes distribution in South-Eastern Tuscany: a ten-year retrospective study. 托斯卡纳东南部的丙型肝炎病毒 (HCV) 基因型分布：一项为期十年的回顾性研究。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Agostino Ognibene, Giulio Camarlinghi, Maria Lorubbio, Manuela Mafucci, Alessandro Saracini, Alessandro Pancrazzi, Silvana Gervino, Gianluca Viti, Augusto Maddalena, Danilo Tacconi, Eva Maria Parisio, Francesca Borgia, Giampiero Caldarelli, Simona Dei, Assunta De Luca, Antonio D'Urso

{"title":"Hepatitis C Virus (HCV) genotypes distribution in South-Eastern Tuscany: a ten-year retrospective study.","authors":"Agostino Ognibene, Giulio Camarlinghi, Maria Lorubbio, Manuela Mafucci, Alessandro Saracini, Alessandro Pancrazzi, Silvana Gervino, Gianluca Viti, Augusto Maddalena, Danilo Tacconi, Eva Maria Parisio, Francesca Borgia, Giampiero Caldarelli, Simona Dei, Assunta De Luca, Antonio D'Urso","doi":"","DOIUrl":"","url":null,"abstract":"Italy is recognized as having the highest Hepatitis C virus (HCV) prevalence in Europe. The Tuscany region, where the prevalence of HCV infection is approximately 0.8%, implemented two programs for the control of chronic hepatitis C in Tuscany from 2018 to 2022. This retrospective study aims to investigate the incidence of HCV in a population screened in Southeastern Tuscany from 2013 to 2022. The study population included 246,137 patients from the provincial area of Arezzo and Grosseto, Tuscany, spanning from January 2013 to October 2022. Among the subjects included in the study, 3,190 (1.29%) tested positive for anti-HCV antibodies. Of this population, 2,119 patients (66.43%) also tested positive for HCV-RNA quantification, leading to their enrolment for subsequent viral genotyping. 1,106 patients had genotype (GT) 1 (52.2%), 484 had GT 3 (22.8%), 371 had GT 2 (17.5%), and 158 had GT 4 (7.5%). Our study underscores the prevalence of HCV GTs 1 and 3 as the most predominant GTs in the Southeast Tuscany region. We also observe a correlation between age, sex and HCV genotypic distribution.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"172-179"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peritoneal dialysis-related peritonitis caused by Stephanoascus ciferrii: A Case Report. Stephanoascus ciferrii 引起的腹膜透析相关腹膜炎：病例报告。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Wenchen Guo, Ting Guo, Yaowen Wang, Wanxiang Li

{"title":"Peritoneal dialysis-related peritonitis caused by Stephanoascus ciferrii: A Case Report.","authors":"Wenchen Guo, Ting Guo, Yaowen Wang, Wanxiang Li","doi":"","DOIUrl":"","url":null,"abstract":"Stephanoascus ciferrii, a conditional pathogenic fungus prevalent in nature, is more frequently encountered in patients with compromised immunity. However, the literature rarely reports infections caused by Stephanoascus ciferrii in peritoneal dialysis patients. Here, we detail the case of a 66-year-old female suffering from renal failure who experienced catheter-related infection during peritoneal dialysis. Dialysate turbidity prompted the detection of Stephanoascus ciferrii in both peritoneal dialysate and tubes through microbiological cultures. Subsequent treatment involved antifungal drugs and a transition to hemodialysis, resulting in the disappearance of peritonitis symptoms and the patient's discharge. In recent years, fungal infections, particularly dialysis-related infections, are on the rise. This marks the first reported case of catheter-related peritonitis infection caused by Stephanoascus ciferrii. Compared to bacterial infections, fungal infections pose challenges due to limited drug options, significant side effects, and prolonged treatment durations. Hence, prompt pathogen diagnosis and drug sensitivity testing are crucial for effective clinical treatment. In essence, this scientific case report underscores the uncommon occurrence of catheter-related peritonitis attributed to Stephanoascus ciferrii in a peritoneal dialysis patient with renal failure, emphasizing the distinctive management challenges and underscoring the critical significance of prompt diagnosis and suitable intervention in such instances.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"186-189"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Etiological structure and antibiotic resistance pattern of burn wound infections in hospitalized patients - a one-center study in Varna, Bulgaria. 住院患者烧伤创面感染的病因结构和抗生素耐药性模式--保加利亚瓦尔纳的一项单中心研究。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Gergana Nedelcheva, Dajana Dobreva, Teofan Kuyumdzhiev, Gabriela Tsankova, Tatina Todorova

{"title":"Etiological structure and antibiotic resistance pattern of burn wound infections in hospitalized patients - a one-center study in Varna, Bulgaria.","authors":"Gergana Nedelcheva, Dajana Dobreva, Teofan Kuyumdzhiev, Gabriela Tsankova, Tatina Todorova","doi":"","DOIUrl":"","url":null,"abstract":"In the present retrospective study, we have evaluated bacterial pathogens isolated from patients admitted to the Burn Care Unit at the Military Medical Academy, Varna, Bulgaria over a three-year period (January 2019 - December 2021). We also tried to summarize the corresponding antibiotic resistance pattern of the isolated infectious agents. A total of 1030 isolates were obtained from 1912 burn wound samples investigated. There were 553 Gram-positive (53.7%) and 477 Gram-negative (46.3%) isolates. The most common isolates for the study period were coagulase-negative staphylococci (CoNS) (25%), Pseudomonas aeruginosa (17.7%), Staphylococcus aureus (16.6%), Acinetobacter baumannii (7.7%), Enterobacter spp. (7.1%), Escherichia coli (4.4%), Proteus spp. (3.4%), and Klebsiella spp. (2.9%). Glycopeptide antibiotics and linezolid were the most effective drugs against gram-positive isolates, followed by amikacin (for synergistic combinations), whereas colistin, imipenem, meropenem, cefoperazon/sulbactam, and piperacillin/tazobactam were the most active drugs against Gram-negative isolates, and colistin, ampicillin/sulbactam - against A. baumannii.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"146-151"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rethinking Combination Therapy for S. aureus bacteremia: A Fading Paradigm. 反思金黄色葡萄球菌菌血症的联合疗法：渐行渐远的范例。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Sergio Venturini, Ingrid Reffo, Manuela Avolio, Giancarlo Basaglia, Giovanni Del Fabro, Astrid Callegari, Igor Bramuzzo, Maurizio Tonizzo, Riccardo Lucis, Elisa Pontoni, Laura De Santi, Massimo Crapis

引用次数: 0

Aerobic vaginitis: antibiotic resistance trend and future actions of antimicrobial diagnostic stewardship. 需氧性阴道炎：抗生素耐药性趋势和抗菌诊断管理的未来行动。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-07-01

Francesco Foglia, Maria Teresa Della Rocca, Fortunato Montella, Maria Vasco, Annalisa Chianese, Carla Zannella, Anna De Filippis, Emiliana Finamore, Massimiliano Galdiero

{"title":"Aerobic vaginitis: antibiotic resistance trend and future actions of antimicrobial diagnostic stewardship.","authors":"Francesco Foglia, Maria Teresa Della Rocca, Fortunato Montella, Maria Vasco, Annalisa Chianese, Carla Zannella, Anna De Filippis, Emiliana Finamore, Massimiliano Galdiero","doi":"","DOIUrl":"","url":null,"abstract":"The study objective is to examine epidemiological and microbiological aspects of aerobic vaginitis in female patients admitted to University Hospital of Campania \"L. Vanvitelli\" over five years. The most represented strains were E. coli (n = 153), Citrobacter spp. increasing from 2020, E. faecalis (n = 149), S. haemolitycus (n = 61), and Candida albicans (n = 87). The susceptibility patterns of a selection of gram-negative and gram-positive representative bacterial isolates were examined. Carbapenems, aminoglycosides, and fosfomycin were most effective against gram-negative bacteria, whereas vancomycin, daptomycin, and linezolid exhibited greater efficacy against gram-positive bacteria. None of the E. coli and Citrobacter spp. isolates produced extended-spectrum beta-lactamases, and the S. haemolyticus strains were methicillin-resistant. In gram-positive isolates, gentamicin susceptibility increased in 2020 and 2021 compared to clindamycin; erythromycin showed high resistance rates in 2020. Our findings indicate that integrating proper microbiological cultures into clinical practice could improve the management of aerobic vaginitis. Moreover, they highlight the necessity of establishing a nationwide surveillance guideline to mitigate antimicrobial resistance. Improvement actions in antimicrobial diagnostic stewardship must be considered when seeking the appropriate diagnosis and treatment for aerobic vaginitis.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 2","pages":"164-171"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141635890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Whole Blood and Plasma for Monitoring Cytomegalovirus and Epstein-Barr Virus. 比较全血和血浆在监测巨细胞病毒和 Epstein-Barr 病毒方面的作用。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Yuto Fukuda, Yuka Torii, Ken-Ichi Iwata, Kazunori Haruta, Makoto Yamaguchi, Takako Suzuki, Atsushi Narita, Hideki Muramatsu, Yasuhiro Ogura, Yoshiyuki Takahashi, Yoshinori Ito, Jun-Ichi Kawada

{"title":"Comparison of Whole Blood and Plasma for Monitoring Cytomegalovirus and Epstein-Barr Virus.","authors":"Yuto Fukuda, Yuka Torii, Ken-Ichi Iwata, Kazunori Haruta, Makoto Yamaguchi, Takako Suzuki, Atsushi Narita, Hideki Muramatsu, Yasuhiro Ogura, Yoshiyuki Takahashi, Yoshinori Ito, Jun-Ichi Kawada","doi":"","DOIUrl":"","url":null,"abstract":"Monitoring Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infection after transplantation is recommended to enable preemptive therapy. However, the most suitable sample type remains unclear. Patients who underwent hematopoietic stem cell or liver transplantation were included in this study. Viral loads in sequential whole-blood and plasma samples were retrospectively analyzed. EBV DNA was detected more frequently in whole blood (55%) than in plasma (18%). The detection rate of CMV DNA was similar between the two sample types. The correlation of viral loads between the two sample types were 0.515 and 0.688 for EBV and CMV, respectively. Among paired samples in which EBV DNA was detected in whole blood, the plasma EBV detection rate was significantly higher in patients who underwent hematopoietic stem cell transplantation than in those who underwent liver transplantation. The viral DNA load in whole blood and plasma showed similar trends. The EBV detection rate was higher in whole blood, and a high correlation was observed between CMV DNA loads and whole blood and plasma. These results indicate that whole blood is more sensitive for monitoring both EBV and CMV, whereas plasma is a potential alternative sample for monitoring CMV.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"52-59"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRESTIGIO RING "a 59-year-old man with multidrug resistant HIV-1 infection failing a regimen including dolutegravir, rilpivirine, atazanavir/cobicistat: successful treatment tailoring based on genotypic and phenotypic resistance tests". PRESTIGIO RING："一名 59 岁的 HIV-1 多药耐药感染者，在使用多鲁曲韦、利匹韦林、阿扎那韦/可比司他治疗方案后失败：根据基因型和表型耐药性测试成功定制了治疗方案"。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Francesca Panza, Francesco Saladini, Niccolò Bartolini, Federica Gianmarino, Francesca Montagnani, Vincenzo Spagnuolo, Mario Tumbarello, Maria Mercedes Santoro, Antonella Castagna, Maurizio Zazzi, Massimiliano Fabbiani

{"title":"PRESTIGIO RING \"a 59-year-old man with multidrug resistant HIV-1 infection failing a regimen including dolutegravir, rilpivirine, atazanavir/cobicistat: successful treatment tailoring based on genotypic and phenotypic resistance tests\".","authors":"Francesca Panza, Francesco Saladini, Niccolò Bartolini, Federica Gianmarino, Francesca Montagnani, Vincenzo Spagnuolo, Mario Tumbarello, Maria Mercedes Santoro, Antonella Castagna, Maurizio Zazzi, Massimiliano Fabbiani","doi":"","DOIUrl":"","url":null,"abstract":"Management of virological failure in heavily treatment-experienced people with multidrug-resistant (MDR) HIV infection is a serious clinical challenge. New drugs with novel mechanisms of action have recently been approved, and their use has improved the outcome of subjects with limited treatment options (LTO). In this setting, the choice of antiretroviral therapy (ART) should be tailored based on the pattern of resistance, treatment history and patients' individual characteristics. While genotypic resistance testing is the reference method for analysing residual drug susceptibility, phenotypic resistance testing can provide additional support when facing LTO. Herein, we present the case of a patient with MDR HIV-1 infection on virological failure enrolled in the PRESTIGIO Registry. The salvage ART regimen, which included drugs with novel mechanisms of action (MoA), was tailored to the patient's clinical characteristics and on the resistance pattern explored with genotypic and phenotypic investigation, allowing the achievement of viro-immunological success. The use of recently approved drugs with novel MoA, combined with an optimized background regimen, may also achieve virological suppression in people with LTO.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"116-122"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of an automated rapid phenotypic antimicrobial susceptibility testing (ASTar, Q-linea AB) applied directly on blood cultures bottles positive for Gram-negative pathogens. 对直接用于革兰氏阴性病原体阳性血培养瓶的自动快速表型抗菌药物敏感性测试（ASTar，Q-linea AB）进行评估。

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-05-01

Isabella Banchini, Eva Caterina Borgatti, Claudio Foschi, Tiziana Lazzarotto, Simone Ambretti

引用次数: 0

Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells. 呼吸道合胞病毒和鼻病毒双重感染会增加人原发性支气管上皮细胞中 IFN-λ1 和 CXCL10 的表达。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-05-01

Nipaporn Sankuntaw, Nuntaya Punyadee, Wasun Chantratita, Viraphong Lulitanond

{"title":"Coinfection with respiratory syncytial virus and rhinovirus increases IFN-λ1 and CXCL10 expression in human primary bronchial epithelial cells.","authors":"Nipaporn Sankuntaw, Nuntaya Punyadee, Wasun Chantratita, Viraphong Lulitanond","doi":"","DOIUrl":"","url":null,"abstract":"Acute respiratory tract infection (ARTI) is common in all age groups, especially in children and the elderly. About 85% of children who present with bronchiolitis are infected with respiratory syncytial virus (RSV); however, nearly one-third are coinfected with another respiratory virus, such as human rhinovirus (HRV). Therefore, it is necessary to explore the immune response to coinfection to better understand the molecular and cellular pathways involving virus-virus interactions that might be modulated by innate immunity and additional host cell response mechanisms. This study aims to investigate the host innate immune response against RSV-HRV coinfection compared with monoinfection. Human primary bronchial/tracheal epithelial cells (HPECs) were infected with RSV, HRV, or coinfected with both viruses, and the infected cells were collected at 48 and 72 hours. Gene expression profiles of IL-6, CCL5, TNF-α, IFN-β, IFN-λ1, CXCL10, IL-10, IL-13, IRF3, and IRF7 were investigated using real-time quantitative PCR, which revealed that RSV-infected cells exhibited increased expression of IL-10, whereas HRV infection increased the expression of CXCL10, IL-10, and CCL5. IFN-λ1 and CXCL10 expression was significantly different between the coinfection and monoinfection groups. In conclusion, our study revealed that two important cytokines, IFN-λ1 and CXCL10, exhibited increased expression during coinfection.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"60-67"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0