New Microbiologica最新文献

Pharmacological determinants of BIC/FTC/TAF to achieve long term success: potency, genetic barrier and forgiveness.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Stefano Bonora

引用次数: 0

Identification of Candida glabrata complex strains isolated from clinical specimens at the species level by molecular methods and determination of their susceptibilities to antifungals.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Yasemin Ayse Ucar, Ozlem Guven, Gonca Erkose Genc

{"title":"Identification of Candida glabrata complex strains isolated from clinical specimens at the species level by molecular methods and determination of their susceptibilities to antifungals.","authors":"Yasemin Ayse Ucar, Ozlem Guven, Gonca Erkose Genc","doi":"","DOIUrl":"","url":null,"abstract":"Candida glabrata can exhibit resistance or reduced susceptibility to fluconazole, and there is an increasing frequency of resistance to echinocandins. Candida nivariensis and Candida bracarensis, newly described in 2005 and 2006, respectively, belong to the C. glabrata complex along with C. glabrata sensu stricto. C. nivariensis and C. bracarensis have been shown to exhibit higher resistance and virulence compared to C. glabrata sensu stricto. These three species cannot be reliably distinguished based on phenotypic characteristics or biochemical-based identification systems. In this study, 40 strains initially identified as C. glabrata using biochemical-based systems were re-identified to the species level through ITS region sequencing. Antifungal susceptibility testing was conducted using the Sensititre YeastOne susceptibility panel. All strains were identified as C. glabrata sensu stricto and showed dose-dependent susceptibility to fluconazole. Of the 40 strains, 39 (97.5%) were susceptible to caspofungin, anidulafungin, and micafungin, while one (2.5%) was resistant. Further research is needed on the epidemiology, virulence factors, and resistance profiles of C. nivariensis and C. bracarensis. Accurate species-level identification and antifungal susceptibility testing of C. glabrata complex isolates are significant.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"344-349"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Epidemiology of Vancomycin-Resistant Enterococci during 2021 in a tertiary care hospital in Greece.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Eleni Panidou-Tsoulou, Georgios Meletis, Charalampos Kotzamanidis, Esmeralda Dushku, Virginia Giantzi, Areti Tychala, Angeliki Kassomenaki, Paraskevi Mantzana, Lemonia Skoura, Efthymia Protonotariou

{"title":"Molecular Epidemiology of Vancomycin-Resistant Enterococci during 2021 in a tertiary care hospital in Greece.","authors":"Eleni Panidou-Tsoulou, Georgios Meletis, Charalampos Kotzamanidis, Esmeralda Dushku, Virginia Giantzi, Areti Tychala, Angeliki Kassomenaki, Paraskevi Mantzana, Lemonia Skoura, Efthymia Protonotariou","doi":"","DOIUrl":"","url":null,"abstract":"The present study aimed to determine the resistance mechanisms and clonal relationships among vancomycin-resistant Enterococci (VRE) isolated in AHEPA University Hospital during the pandemic year 2021. Overall, 140 clinical VRE were isolated during the study period and 44 were randomly selected for molecular analysis. A multiplex PCR was employed to detect vancomycin resistance genes (vanA, vanB, vanC, vanD, vanE, vanG) using specific primers. Additionally, pulsed-field gel electrophoresis (PFGE) was performed to assess the clonal relatedness of the selected isolates. The multiplex PCR showed that 36 of the 44 studied strains (81.9%) harbored the vanA gene whereas, 7 of 44 (15.9%) co-harbored the vanA and vanB resistance genes; one isolate had a negative PCR result. PFGE analysis unveiled 37 distinct electrophoretic patterns among the 44 VRE isolates with a similarity threshold of 75%. These patterns were clustered into 4 distinct branches. Our findings indicate a polyclonal distribution of vanA genes among the studied isolates and the notable concomitant presence of vanA/vanB genotypes. Furthermore, these results highlight the worsening that took place during the COVID-19 pandemic period regarding antibiotic resistance rates, underscoring the imperative need for stringent infection control measures and active surveillance.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"350-354"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candida krusei pericarditis: A case report and review of the literature.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Ahmed Qaedi, Umran Elbahr, Gülcan Abalı, Burcu Çakar, Orhan Şencan, Suha Hejres, Oguz Resat Sipahi

{"title":"Candida krusei pericarditis: A case report and review of the literature.","authors":"Ahmed Qaedi, Umran Elbahr, Gülcan Abalı, Burcu Çakar, Orhan Şencan, Suha Hejres, Oguz Resat Sipahi","doi":"","DOIUrl":"","url":null,"abstract":"A 66-year-old female, a known case of endometrial serous carcinoma, presented with a history of poor oral intake. Elevated C-reactive protein was identified, and an echocardiogram showed evidence of large circumferential pericardial fluid with maximum diameter measuring 4.4 cm. Further analysis of the pericardial fluid revealed growth of Candida krusei. Treatment was initiated with caspofungin 150mg intravenously once daily for 28 days, followed by oral maintenance therapy with voriconazole for 10 days. No relapse or re-infection was observed during 10 days of follow-up. A literature search was performed, which demonstrated that Candida albicans is the most encountered Candida spp. associated with acute pericarditis. Additionally, the following risk factors were observed, including history of major surgery, COVID-19 infection, and use of broad-spectrum antibiotics. This article presents a unique case of acute pericarditis caused by C. krusei. It highlights the importance of identifying the etiology in immunocompromised cohorts and emphasizes the role of establishing formative treatment guidelines for the management of Candida spp. pericarditis.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"355-357"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and survival of extended-spectrum β-lactamase-producing clinical strains of Klebsiella pneumoniae isolated in Mostar, Bosnia and Herzegovina.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Tanja Petrović, Marija Tonkić, Ana Maravić, Mia Dželalija, Željana Fredotović, Jasna Hrenović, Sanja Jakovac, Doris Martinović Rizikalo, Ivana Goić-Barišić

{"title":"Molecular characterization and survival of extended-spectrum β-lactamase-producing clinical strains of Klebsiella pneumoniae isolated in Mostar, Bosnia and Herzegovina.","authors":"Tanja Petrović, Marija Tonkić, Ana Maravić, Mia Dželalija, Željana Fredotović, Jasna Hrenović, Sanja Jakovac, Doris Martinović Rizikalo, Ivana Goić-Barišić","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this retrospective study was to investigate the molecular characteristics and survival ability of clinical isolates of extended-spectrum β-lactamase (ESBL)-producing strains of Klebsiella pneumoniae (K. pneumoniae) in a hospital setting. ESBL-producing K. pneumoniae clinical isolates were collected from hospitalized patients at the University Clinical Hospital of Mostar, Bosnia and Herzegovina. Identification and antibiotic susceptibility tests were performed according to established laboratory methods and Clinical and Laboratory Standards Institute standards. Phenotypic identification of the isolates was confirmed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Minimum inhibitory concentrations were determined using the Vitek® 2 Compact System. Clonal relationships between the different isolates were studied using pulsed-field gel electrophoresis. Molecular characterization of ESBL-coding genes was performed using multiplex polymerase chain reaction. The survival and pellicle formation abilities of ESBL-producing K. pneumoniae isolates were also investigated. Among 471 isolated K. pneumoniae strains, 149 isolates (31.6%) were ESBL-producing. The bla genes encoding CTX-M-1-group ESBLs were identified in almost all isolates, with the majority carrying blaCTX-M-15. Two isolates contained blaCTX-M-3. All isolates carried blaTEM-1. The blaSHV genes identified were blaSHV-1, blaSHV-5, blaSHV-11, and blaSHV-28. Six isolates harbored blaOXA-1. ESBL-producing K. pneumoniae strains survived on dry cotton for up to 49 days.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"337-343"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV cure-related research: where we stand and where we go.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Martina Ranzenigo, Antonella Castagna, Camilla Muccini

{"title":"HIV cure-related research: where we stand and where we go.","authors":"Martina Ranzenigo, Antonella Castagna, Camilla Muccini","doi":"","DOIUrl":"","url":null,"abstract":"This review outlines the progress and challenges in HIV cure research, with a particular focus on gene therapy, latency-reversing agents (LRAs), immunotherapy, and stem cell transplants. The objective of these advances is to achieve either a sterilizing or a functional cure, whereby the virus is either eradicated or controlled without the need for ongoing treatment. Gene editing tools, such as CRISPR-Cas9, have demonstrated the potential to remove HIV DNA from cells. LRAs, on the other hand, aim to reactivate latent virus, thereby rendering it susceptible to treatment or immune clearance. Immunotherapy, including therapeutic vaccines and broadly neutralizing antibodies, aims to enhance the immune response to HIV. However, achieving long-term suppression without antiretroviral therapy remains a significant challenge. Stem cell transplants have resulted in instances of remission; however, the methodology is intricate, perilous, and viable solely for patients with particular medical requirements. Each approach encounters challenges pertaining to safety, administration, and accessibility. In sum, while considerable advancement has been achieved, a combination of therapeutic strategies and further innovations are imperative for the development of a sustainable, widely accessible HIV cure.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"303-310"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A serious hepatitis E case in the city of Rieti (Lazio, Italy): correlation with a 2019 cluster suggests a resident hepatitis E virus strain; a case report.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Mauro Zaccarelli, Claudia Minosse, Silvia Pauciullo, Massimiliano Scuderi, Fabio Gemelli, Giuseppe Tacchi, Giovanna Giacchetti, Mauro Marchili, Valentina D'Ovidio, Anna Rosa Garbuglia

引用次数: 0

Clarithromycin-resistant Helicobacter pylori in Ankara, Turkey: Detection by real-time PCR in formalin-fixed paraffin-embedded gastric biopsy samples.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Betül Yüzügüldü, Yusuf Üstün, Sehbal Arslankoz, Eylül Beren Tanık, Ayfer Bakır, Murat Alper

{"title":"Clarithromycin-resistant Helicobacter pylori in Ankara, Turkey: Detection by real-time PCR in formalin-fixed paraffin-embedded gastric biopsy samples.","authors":"Betül Yüzügüldü, Yusuf Üstün, Sehbal Arslankoz, Eylül Beren Tanık, Ayfer Bakır, Murat Alper","doi":"","DOIUrl":"","url":null,"abstract":"Choosing the appropriate treatment regimen for Helicobacter pylori (Hp) depends on knowing or predicting regional antibiotic resistance rates. In this study, we aimed to determine the frequency of Hp and clarithromycin (Cla) resistance rates by PCR analysis in formalin-fixed paraffin-embedded (FFPE) gastric biopsies of patients diagnosed with gastritis. Hp DNA and Hp Cla resistance (ClaR) were analyzed by PCR method. Diagnostic examination of gastric biopsy samples was evaluated according to the Updated Sydney System. Hp positivity was detected in 68 of 149 samples (45.6%). ClaR was detected in 29 Hp-positive samples (42.6%). A decrease in the resistance rate to Cla in HP was observed with increasing age (p = 0.08). The risk of resistance was higher in women (OR 1.1, 95% CI: 0.41-2.90, p = 0.86). The ClaR was higher in the 18-40 age group than in the over-60 age group (OR 5.5, 95% CI: 1.16-26.14, p = 0.03). The ClaR rate was found to be 42.6% by molecular method, indicating that classical triple therapy containing Cla is not a suitable option. Since resistance may vary according to geographical regions and years, regional studies with large populations are needed to determine antibiotic resistance rates before treatment planning.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"324-329"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical analysis of meningitis in neonates infected with ESBL-producing Escherichia coli: a retrospective study.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Weitang Huang, Liuqing Ye, Guangming Chen, Qiongdan Fan, Hanbing Yu

{"title":"Clinical analysis of meningitis in neonates infected with ESBL-producing Escherichia coli: a retrospective study.","authors":"Weitang Huang, Liuqing Ye, Guangming Chen, Qiongdan Fan, Hanbing Yu","doi":"","DOIUrl":"","url":null,"abstract":"Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are widely distributed in the community. Notably, ESBL-producing E. coli have high-frequency early mother-to-child transmission. The current study determined the treatment and intervention measures among neonates with ESBL-producing E. coli meningitis. Five neonates (4 males and 1 female) with meningitis caused by ESBL-producing E. coli admitted to our center from June 2020 to July 2023 were retrospectively reviewed. The clinical characteristics and treatment effects were analyzed. There were 4 cases of early- onset sepsis and 1 case of a catheter-related bloodstream infection. Four of 5 cases were premature infants with gestational ages between 27 and 33 weeks. The clinical manifestations included fevers (n=5), lethargy (n=5), dyspnea (n=4), and microcirculation disorders (n=4). Meropenem was infused intravenously in 2 patients with a median duration of 120.5 d (range, 98-143 d) at an early dose of 40 mg/kg every 12 h and an extended infusion. Three patients were infused with 40 mg/kg every 8 h for a median duration of 28 d (range, 21-61 d). Two neonates had recurrent illnesses, three neonates developed hydrocephalus, one neonate had a cerebral hemorrhage, and one neonate had convulsions during treatment. The follow-up results showed 4 neonates had normal neuromotor development and 1 neonate had a 2-month lag in neuromotor development. Maternal perinatal infections, premature delivery, and catheter-related bloodstream infections are high-risk factors for ESBL-producing E. coli meningitis. Children with fevers and lethargy should be monitored closely. ESBL-producing E. coli infections in children with meningitis are prone to recurrences and secondary hydrocephalus. Attention should be paid to ensuring the correct high dose of the antibiotic.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 4","pages":"330-336"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Status of Alkhurma Hemorrhagic Fever Virus Infection: Future Concerns and Considerations.

IF 1.5 4区医学

New Microbiologica Pub Date : 2024-12-01

Nada Mohammed Aljuaid, Magdah Ali Ganash, Sayed Sartaj Sohrab, Esam Ibraheem Azhar

引用次数: 0