基于rRVFV-NP间接ELISA检测裂谷热病毒IgG的研究

IF 1.5 4区 医学 Q4 MICROBIOLOGY
New Microbiologica Pub Date : 2025-05-01
Arwa A Zehairy, Sayed S Sohrab, Thamir A Alandijany, Ahmed M Hassan, Aymn T Abbas, Umama A Abdel-Dayem, Awatif A Al-Judaibi, Esam I Azhar
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引用次数: 0

摘要

裂谷热病毒(RVFV)是一种人畜共患虫媒病毒,可在人类和多种家畜中引起重大传染病。2000年,沙特阿拉伯和也门发生了一次引人注目的裂谷热病毒流行,造成了严重的公共卫生后果以及人类和动物的高死亡率。尽管形势紧迫,但迄今尚未开发出经批准的疫苗或用于人类的特定抗病毒治疗。目前还没有商用的裂谷热病毒ELISA检测方法。本研究旨在利用纯化的重组RVFV核蛋白(NP)开发一种内部ELISA。利用屠宰场工人的232份血清样本对该检测方法进行优化,并针对SNT进行了验证。当使用RVFV-NP作为包被抗原时,所建立的检测方法特异性为96.64%,灵敏度为100%,临界值为0.36。相比之下,市售的基于rvfv - np的ELISA特异性为96.23%,灵敏度为100%,临界值为0.37。此外,阳性样品的吸光度值与其相应的SNT滴度呈显著相关。本研究开发的内部ELISA为裂谷热病毒提供了一种强大的诊断和筛查工具。它为早期发现、监测和控制裂谷热病毒感染提供了宝贵的机制,从而有助于有效管理和预防未来的疫情。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Developing Indirect rRVFV-NP based ELISA Protocol for Detection of IgG Against Rift Valley Fever Virus.

Rift Valley fever virus (RVFV) is a zoonotic arbovirus that causes significant infectious diseases in humans and in a wide array of domestic livestock. A notable epidemic of RVFV occurred in Saudi Arabia and Yemen in 2000, resulting in severe public health ramifications and high mortality rates among both human and animal populations. Despite the urgency of the situation, no approved vaccine or specific antiviral treatment for human use has been developed to date. There are currently no commercially available ELISA assays for RVFV. This study aims to develop an in-house ELISA utilizing purified recombinant nucleoprotein (NP) of RVFV. A total of 232 serum samples from slaughterhouse workers were employed to optimize this assay, which was validated against the SNT. The developed assay demonstrated a specificity of 96.64% and a sensitivity of 100% with a cut-off value of 0.36 when using the in-house RVFV-NP as the coating antigen. In comparison, a commercially available RVFV-NP-based ELISA exhibited a specificity of 96.23% and a sensitivity of 100% with a cut-off value of 0.37. Furthermore, the absorbance values of positive samples showed a significant correlation with their corresponding SNT titers. The in-house ELISA developed in this study offers a robust diagnostic and screening tool for RVFV. It provides a valuable mechanism for early detection, surveillance, and control of RVFV infections, thereby contributing to effective management and prevention of future outbreaks.

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来源期刊
New Microbiologica
New Microbiologica 生物-微生物学
CiteScore
2.20
自引率
5.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.
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