CRISPR Journal最新文献

The Lexicon of CRISPR: When Is It Too Much? CRISPR词汇：什么时候太过了？

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-09-29 DOI: 10.1177/25731599251385430

Rodolphe Barrangou

引用次数: 0

A Phage Variable Region Encodes Anti-CRISPR Proteins Inhibiting All Streptococcus thermophilus CRISPR Immune Systems. 噬菌体可变区编码抗CRISPR蛋白抑制所有嗜热链球菌CRISPR免疫系统。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-09-29 DOI: 10.1177/25731599251369720

Katie A Johnson, Clare Cooper, Cécile Philippe, Ryan J Catchpole, Shakela Mitchell, Michael P Terns

{"title":"A Phage Variable Region Encodes Anti-CRISPR Proteins Inhibiting All Streptococcus thermophilus CRISPR Immune Systems.","authors":"Katie A Johnson, Clare Cooper, Cécile Philippe, Ryan J Catchpole, Shakela Mitchell, Michael P Terns","doi":"10.1177/25731599251369720","DOIUrl":"https://doi.org/10.1177/25731599251369720","url":null,"abstract":"Bacteria and archaea utilize CRISPR-Cas systems to defend against invading mobile genetic elements (MGEs) such as phages and plasmids. In turn, MGEs have evolved anti-CRISPR (Acr) proteins to counteract these defenses. While several type II-A Acrs have been identified in Streptococcus thermophilus (Sth) phages, a more comprehensive understanding of Acr diversity in Sth phages has yet to be explored. Guided by the genomic context of known Acrs, we systematically screened uncharacterized phage proteins and identified several novel Acrs that inhibit type I-E, type II-A or type III-A Sth CRISPR-Cas systems. These acr genes are clustered within a variable phage genomic region, indicating a hotspot for anti-defense activity. We also identified neighboring proteins with predicted enzymatic or structural domains that may modulate phage-host interactions through Acr-independent mechanisms. Together, our findings expand the known repertoire of Sth Acrs and highlight the phage variable region as a key reservoir of immune-modulating factors.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient CRISPR-Cas9-Mediated Genome Editing of the Cane Toad (Rhinella marina). 高效crispr - cas9介导的甘蔗蟾蜍基因组编辑

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-09-26 DOI: 10.1177/25731599251382427

Michael B Clark, Alexander T Funk, Alex Paporakis, Gregory P Brown, Samuel J Beach, Aidan Tay, Stephanie Deering, Caitlin Cooper, Mark Tizard, Chris J Jolly, Georgia Ward-Fear, Anthony W Waddle, Richard Shine, Maciej Maselko

{"title":"Efficient CRISPR-Cas9-Mediated Genome Editing of the Cane Toad (Rhinella marina).","authors":"Michael B Clark, Alexander T Funk, Alex Paporakis, Gregory P Brown, Samuel J Beach, Aidan Tay, Stephanie Deering, Caitlin Cooper, Mark Tizard, Chris J Jolly, Georgia Ward-Fear, Anthony W Waddle, Richard Shine, Maciej Maselko","doi":"10.1177/25731599251382427","DOIUrl":"https://doi.org/10.1177/25731599251382427","url":null,"abstract":"Invasive species inflict major ecological, economic, and cultural harm worldwide, highlighting the urgent need for innovative control strategies. Genome editing offers exciting possibilities for targeted control methods for invasive species. Here, we demonstrate CRISPR-Cas9 genome editing in the cane toad (Rhinella marina), one of Australia's most notorious invasive species, by targeting the tyrosinase gene to produce albino phenotypes as visual markers for assessing editing efficiency. Microinjection of Cas9 protein and guide RNAs into one-cell zygotes resulted in 87.6% of mosaic larvae displaying nearly complete albinism, with 2.3% exhibiting complete albinism. For completely albino individuals, genomic analysis confirmed predominantly frameshift mutations or large deletions at the target site, with no wild-type alleles detected. Germline transmission rates reflected the extent of albinism in the mosaic adult, with maternal transmission approaching 100%. This first application of CRISPR-Cas9 in the Bufonidae family opens possibilities for exploring basic research questions and population control strategies.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Installation of Heterozygous Mutations in Human Pluripotent Stem Cells Using Prime Editing. 利用先导编辑技术在人类多能干细胞中高效安装杂合突变。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-09-24 DOI: 10.1177/25731599251380122

Annabelle Suter, Alison Graham, Jia Yi Kuah, Jason Crisologo, Chathuni Gunatilake, Koula Sourris, Michael See, Fernando J Rossello, Mirana Ramialison, Katerina Vlahos, Sara E Howden

{"title":"Efficient Installation of Heterozygous Mutations in Human Pluripotent Stem Cells Using Prime Editing.","authors":"Annabelle Suter, Alison Graham, Jia Yi Kuah, Jason Crisologo, Chathuni Gunatilake, Koula Sourris, Michael See, Fernando J Rossello, Mirana Ramialison, Katerina Vlahos, Sara E Howden","doi":"10.1177/25731599251380122","DOIUrl":"https://doi.org/10.1177/25731599251380122","url":null,"abstract":"The utility of human pluripotent stem cells (hPSCs) is greatly enhanced by the ability to introduce precise, site-specific genetic modifications with minimal off-target effects. Although Cas9 endonuclease is an exceptionally efficient gene-editing tool, its propensity for generating biallelic modifications often limits its capacity for introducing heterozygous variants. Here, we use prime editing (PE) to install heterozygous edits in over 10 distinct genetic loci, achieving knock-in efficiencies of up to 40% without the need for subsequent purification or drug selection steps. Moreover, PE enables the precise introduction of heterozygous edits in paralogous genes that are otherwise extremely challenging to achieve using endonuclease-based editing approaches. We also show that PE can be successfully combined with reprogramming to derive heterozygous induced pluripotent stem cell clones directly from human fibroblasts and peripheral blood mononuclear cells. Our findings highlight the utility of PE for generating hPSCs with complex edits and represent a powerful platform for modeling disease-associated dominant mutations and gene-dosage effects in an entirely isogenic context.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Prime Editing in Zebrafish. 优化斑马鱼的Prime编辑。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-09-19 DOI: 10.1177/25731599251380500

Rehman Basharat, Gina Rizzo, Josiah D Zoodsma, Lonnie P Wollmuth, Howard I Sirotkin

引用次数: 0

CRISPR-Cas9 Single Nucleotide Editing of Tuberous Sclerosis Complex 2 Gene in Mesenchymal Stem Cells. 间充质干细胞中结节性硬化复合体2基因的CRISPR-Cas9单核苷酸编辑。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-08-14 DOI: 10.1177/25731599251367059

Abdallah Salemdawod, Brandon Cooper, Yajie Liang, Piotr Walczak, Hartmut Vatter, Jaroslaw Maciaczyk, Miroslaw Janowski

{"title":"CRISPR-Cas9 Single Nucleotide Editing of Tuberous Sclerosis Complex 2 Gene in Mesenchymal Stem Cells.","authors":"Abdallah Salemdawod, Brandon Cooper, Yajie Liang, Piotr Walczak, Hartmut Vatter, Jaroslaw Maciaczyk, Miroslaw Janowski","doi":"10.1177/25731599251367059","DOIUrl":"https://doi.org/10.1177/25731599251367059","url":null,"abstract":"The tuberous sclerosis complex (TSC)2 gene regulates the mammalian target of rapamycin (mTOR) pathway, impacting cell proliferation and growth. The loss-of-function mutations, especially in mesenchymal progenitors, drive the development multiple benign and malignant tumors. TSC2 mutations in certain cancer types, e.g., breast cancer, are also associated with poorer prognosis. The databases of TSC2-mutations report point mutations as the most prevalent. We aimed to test the feasibility of inducing point mutations in mesenchymal stem cells (MSCs), targeting the most frequent point mutations of the TSC2 gene, TSC2. c.1864 C>T (p.Arg622Trp), TSC2. c.1832 G>A (p.Arg611Glu), and TSC2. c.5024 C>T (p.Pro1675Leu) using two delivery methods for CRISPR-Cas9. We report a high editing efficiency of up to 85% inducing TSC2 point mutations in hMSCs using lipofectamine-based transfection. Overall, the high editing efficiency of some TSC2 mutations enables the induction and reversal of mutations in primary hMSCs without needing resource-consuming derivation of cell lines frequently distinct from their primary counterparts.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible Reversion of CRISPR-Cas9-Edited Sequences in Octoploid Strawberry. 八倍体草莓中crispr - cas9编辑序列的可能逆转。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-08-11 DOI: 10.1177/25731599251361374

Xiangyi Sun, Maofu Li, Hua Wang, Yuan Yang, Yanhui Kang, Pei Sun, Jing Dong, Min Jin, Wanmei Jin

{"title":"Possible Reversion of CRISPR-Cas9-Edited Sequences in Octoploid Strawberry.","authors":"Xiangyi Sun, Maofu Li, Hua Wang, Yuan Yang, Yanhui Kang, Pei Sun, Jing Dong, Min Jin, Wanmei Jin","doi":"10.1177/25731599251361374","DOIUrl":"https://doi.org/10.1177/25731599251361374","url":null,"abstract":"Gene editing is more challenging in octoploids due to the presence of multiple copies of each gene. However, the ability to edit genes in these plants would allow editing in commercial varieties. Here, we delivered sequences targeting FaMYB9 into octoploid strawberry \"Honeoye\" and identified several gene-edited lines. Among them, the heterozygous gene-edited line FaMYB9CR-15 had curved and wrinkled leaves at 3 months, whereas leaves of 3-month-old wild-type (WT) strawberry seedlings were elliptical with a smooth surface. At that stage, FaMYB9CR-15 leaves also had large patches of wax. We identified 11,402 differentially expressed genes, divided into four clusters, between WT and FaMYB9CR-15 seedlings at 3 months. Notably, cluster 4 genes-related to nonhomologous end joining, microhomology-mediated end joining repairs, homologous recombination, nucleotide excision repair, and mismatch repair-were more highly expressed in the gene-edited line than in the WT. Surprisingly, by 6 months of age, FaMYB9CR-15 leaves had become smooth with small patches of wax, and expression levels of cluster 4 genes were significantly lower than at 3 months. Over the same period, the percentage of FaMYB9 loci harboring the mutant allele decreased from 70.2% to 43.7%. These findings lead us to conclude that there could be reversion of mutated sequences in octoploid strawberry, emphasizing the challenges of gene editing high-ploidy materials.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnologies in the World: On Global Asymmetries and the Need for Cosmopolitanism. 世界上的生物技术：关于全球不对称和世界主义的需要。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-08-01 Epub Date: 2025-05-21 DOI: 10.1089/crispr.2025.0054

Kaushik Sunder Rajan

引用次数: 0

Science with Society at Science Gallery Bengaluru. 科学与社会在班加罗尔科学画廊。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-08-01 Epub Date: 2025-05-20 DOI: 10.1089/crispr.2025.0052

Jahnavi Phalkey

引用次数: 0

Summitting CRISPR for Human Heritable Genome Editing. 人类可遗传基因组编辑CRISPR峰会。