CRISPR Journal最新文献_第10页

Whole-Genome Sequencing Reveals Rare Off-Target Mutations in MC1R-Edited Pigs Generated by Using CRISPR-Cas9 and Somatic Cell Nuclear Transfer. 全基因组测序揭示了利用 CRISPR-Cas9 和体细胞核移植技术生成的 MC1R 编辑猪的罕见脱靶突变。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0034

Zhenyang Li, Jin Lan, Xuan Shi, Tong Lu, Xiaoli Hu, Xiaohong Liu, Yaosheng Chen, Zuyong He

引用次数: 0

Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai). 在养殖的太平洋鲍鱼（Haliotis discus hannai）中进行易于使用的 CRISPR-Cas9 基因组编辑。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0070

Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang

{"title":"Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai).","authors":"Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang","doi":"10.1089/crispr.2023.0070","DOIUrl":"10.1089/crispr.2023.0070","url":null,"abstract":"The Pacific abalone is an important aquaculture shellfish and serves as an important model in basic biology study. However, the study of abalone is limited by lack of highly efficient and easy-to-use gene-editing tools. In this paper, we demonstrate efficient gene knockout in Pacific abalone using CRISPR-Cas9. We developed a highly effective microinjection method by nesting fertilized eggs in a low-concentration agarose gel. We identified the cilia developmental gene β-tubulin and light-sensitive transmembrane protein r-opsin as target genes and designed highly specific sgRNAs for modifying their genomic sequences. Sanger sequencing of the genomic regions of β-tubulin and r-opsin genes from injected larvae identified various genomic long-fragment deletions. In situ hybridization showed gene expression patterns of β-tubulin and r-opsin were significantly altered in the mosaic mutants. Knocking out β-tubulin in abalone embryos efficiently affected cilia development. Scanning electron microscopy and swimming behavior assay showed defecting cilia and decreased motility. Moreover, knocking out of r-opsin in abalone embryos effectively affected the expression and development of eyespots. Overall, this work developed an easy-to-use mosaic gene knockout protocol for abalone, which will allow researchers to utilize CRISPR-Cas9 approaches to study unexploited abalone biology and will lead to novel breeding methods for this aquaculture species.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"7 1","pages":"41-52"},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors. 使用基于CRISPR-Cas9的胞嘧啶和腺嘌呤碱基编辑器编辑果蝇的种系。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-02 DOI: 10.1089/crispr.2023.0026

Nirav Thakkar, Adela Hejzlarova, Vaclav Brabec, David Dolezel

引用次数: 0

Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISPR Journal, 2023;6(5):462-472; DOI: 10.1089/crispr.2023.0021. 更正：CRISPR定位编辑的基因分型多重测序（GMUSCLE）：张等人分析CRISPR编辑细胞的实验和计算方法。CRISPR期刊，2023；6（5）:462-472；DOI:10.1089/crispr.2023.0021。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-08 DOI: 10.1089/crispr.2023.0021.correx

引用次数: 0

Special Issue: CRISPR Trials. 特刊：CRISPR 试验。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 DOI: 10.1089/crispr.2023.29166.cfp2

Fyodor Urnov

引用次数: 0

The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene. 利用Addgene扩大CRISPR质粒的传播和分布模式。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-22 DOI: 10.1089/crispr.2023.0059

Brook Pyhtila, Seth Kasowitz, Rachel Leeson, Rodolphe Barrangou

{"title":"The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene.","authors":"Brook Pyhtila, Seth Kasowitz, Rachel Leeson, Rodolphe Barrangou","doi":"10.1089/crispr.2023.0059","DOIUrl":"10.1089/crispr.2023.0059","url":null,"abstract":"CRISPR-based technologies have rapidly enabled the democratization of genome editing in academic institutions through distribution by Addgene over the past decade. Recently, several distribution milestones have been reached, with a collection of >15,000 plasmids deposited by >1,000 laboratories spanning ∼40 countries now shipped 300,000 times to ∼5,000 organizations traversing ∼100 countries. Yet, both deposits of and requests for CRISPR plasmids continue to rise for this disruptive technology. Distribution patterns revealed robust demand for three distinct classes of CRISPR effectors, namely nucleases (e.g., Cas9 and Cas12), modulators (deactivated CRISPR nucleases fused to transcriptional regulators and epigenome modifiers), and chimeric effectors (Cas proteins fused to enzymes carrying out other activities such as deamination, reverse transcription, transposition, and integration). Yearly deposits over the past decade are requested in near-even proportions, reflecting continuous technological development and requests for novel constructs. Though it is unclear whether the slowing rate of requests is inherent to a pandemic operational lag or a transition from emerging to mature technology, it is noteworthy that the relative proportion of requests from plasmids deposited in the previous year remains stable, suggesting robust development of novel tools concurrent with continued adoption of editing, base editing, prime editing, and more. Predictably, most requested plasmids are designed for mammalian genome manipulation, presumably for medical research and human health pursuits, reflecting investments in therapeutic applications. Concurrently, requests for plant and microbial constructs are on the rise, especially in regions of the world more reliant on local agricultural inputs and focused on food and feed applications, illustrating continued diversification of genome editing applications.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":"493-501"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10753985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Antiviral Sensor Targets Infected Mosquitoes. 工程抗病毒传感器锁定受感染的蚊子

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 DOI: 10.1089/crispr.2023.0056

Elena Dalla Benetta, Adam J López-Denman, Hsing-Han Li, Reem A Masri, Daniel J Brogan, Michelle Bui, Ting Yang, Ming Li, Michael Dunn, Melissa J Klein, Sarah Jackson, Kyle Catalan, Kim R Blasdell, Priscilla Tng, Igor Antoshechkin, Luke S Alphey, Prasad N Paradkar, Omar S Akbari

{"title":"Engineered Antiviral Sensor Targets Infected Mosquitoes.","authors":"Elena Dalla Benetta, Adam J López-Denman, Hsing-Han Li, Reem A Masri, Daniel J Brogan, Michelle Bui, Ting Yang, Ming Li, Michael Dunn, Melissa J Klein, Sarah Jackson, Kyle Catalan, Kim R Blasdell, Priscilla Tng, Igor Antoshechkin, Luke S Alphey, Prasad N Paradkar, Omar S Akbari","doi":"10.1089/crispr.2023.0056","DOIUrl":"10.1089/crispr.2023.0056","url":null,"abstract":"Escalating vector disease burdens pose significant global health risks, as such innovative tools for targeting mosquitoes are critical. CRISPR-Cas technologies have played a crucial role in developing powerful tools for genome manipulation in various eukaryotic organisms. Although considerable efforts have focused on utilizing class II type II CRISPR-Cas9 systems for DNA targeting, these modalities are unable to target RNA molecules, limiting their utility against RNA viruses. Recently, the Cas13 family has emerged as an efficient tool for RNA targeting; however, the application of this technique in mosquitoes, particularly Aedes aegypti, has yet to be fully realized. In this study, we engineered an antiviral strategy termed REAPER (vRNA Expression Activates Poisonous Effector Ribonuclease) that leverages the programmable RNA-targeting capabilities of CRISPR-Cas13 and its potent collateral activity. REAPER remains concealed within the mosquito until an infectious blood meal is uptaken. Upon target viral RNA infection, REAPER activates, triggering programmed destruction of its target arbovirus such as chikungunya. Consequently, Cas13-mediated RNA targeting significantly reduces viral replication and viral prevalence of infection, and its promiscuous collateral activity can even kill infected mosquitoes within a few days. This innovative REAPER technology adds to an arsenal of effective molecular genetic tools to combat mosquito virus transmission.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"6 6","pages":"543-556"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11085028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138807971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa. CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa.

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 DOI: 10.1089/crispr.2023.0039

Erin R Burnight, Luke A Wiley, Nathaniel K Mullin, Malavika K Adur, Mallory J Lang, Cathryn M Cranston, Chunhua Jiao, Stephen R Russell, Elliot H Sohn, Ian C Han, Jason W Ross, Edwin M Stone, Robert F Mullins, Budd A Tucker

{"title":"CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa.","authors":"Erin R Burnight, Luke A Wiley, Nathaniel K Mullin, Malavika K Adur, Mallory J Lang, Cathryn M Cranston, Chunhua Jiao, Stephen R Russell, Elliot H Sohn, Ian C Han, Jason W Ross, Edwin M Stone, Robert F Mullins, Budd A Tucker","doi":"10.1089/crispr.2023.0039","DOIUrl":"10.1089/crispr.2023.0039","url":null,"abstract":"Rhodopsin (RHO) mutations such as Pro23His are the leading cause of dominantly inherited retinitis pigmentosa in North America. As with other dominant retinal dystrophies, these mutations lead to production of a toxic protein product, and treatment will require knockdown of the mutant allele. The purpose of this study was to develop a CRISPR-Cas9-mediated transcriptional repression strategy using catalytically inactive Staphylococcus aureus Cas9 (dCas9) fused to the Krüppel-associated box (KRAB) transcriptional repressor domain. Using a reporter construct carrying green fluorescent protein (GFP) cloned downstream of the RHO promoter fragment (nucleotides -1403 to +73), we demonstrate a ∼74-84% reduction in RHO promoter activity in RHOpCRISPRi-treated versus plasmid-only controls. After subretinal transduction of human retinal explants and transgenic Pro23His mutant pigs, significant knockdown of rhodopsin protein was achieved. Suppression of mutant transgene in vivo was associated with a reduction in endoplasmic reticulum (ER) stress and apoptosis markers and preservation of photoreceptor cell layer thickness.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"6 6","pages":"502-513"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138807791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors. 一种荧光报告小鼠，用于在体内评估使用多种 Cas 核酸酶和主编辑器进行基因组编辑的效果。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 DOI: 10.1089/crispr.2023.0048

Zexiang Chen, Suet-Yan Kwan, Aamir Mir, Max Hazeltine, Minwook Shin, Shun-Qing Liang, Io Long Chan, Karen Kelly, Krishna S Ghanta, Nicholas Gaston, Yueying Cao, Jun Xie, Guangping Gao, Wen Xue, Erik J Sontheimer, Jonathan K Watts

引用次数: 0

First Drug Approval Rises the CRISPR Tide. 首次药物批准掀起 CRISPR 浪潮。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-12-05 DOI: 10.1089/crispr.2023.29168.editorial

Rodolphe Barrangou

引用次数: 0