利用Addgene扩大CRISPR质粒的传播和分布模式。

IF 3.7 4区 生物学 Q2 GENETICS & HEREDITY
CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-22 DOI:10.1089/crispr.2023.0059
Brook Pyhtila, Seth Kasowitz, Rachel Leeson, Rodolphe Barrangou
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引用次数: 0

摘要

在过去的十年里,通过Addgene的分销,基于crispr的技术迅速实现了学术机构基因组编辑的民主化。最近,已经达到了几个分销里程碑,跨越~ 40个国家的>1,000个实验室存放的>15,000个质粒现已运送30万次到跨越~ 100个国家的~ 5,000个组织。然而,对CRISPR质粒的储备和需求都在继续增加,因为这项颠覆性的技术。分布模式显示了对三种不同类型的CRISPR效应物的强劲需求,即核酸酶(例如Cas9和Cas12),调节剂(失活的CRISPR核酸酶融合到转录调节剂和表观基因组修饰剂中)和嵌合效应物(Cas蛋白融合到执行其他活动的酶中,如脱氨、逆转录、转位和整合)。在过去的十年中,每年的存款要求几乎均匀,反映了持续的技术发展和对新结构的要求。虽然尚不清楚请求速度放缓是大流行操作滞后还是新兴技术向成熟技术的过渡所固有的,但值得注意的是,上一年沉积的质粒请求的相对比例保持稳定,这表明在继续采用编辑、碱基编辑、主要编辑等方法的同时,新工具的强劲发展。可以预见的是,大多数要求的质粒是为哺乳动物基因组操作而设计的,可能是为了医学研究和人类健康追求,反映了对治疗应用的投资。与此同时,对植物和微生物构建物的需求也在增加,特别是在世界上更依赖当地农业投入并侧重于食品和饲料应用的地区,这表明基因组编辑应用的持续多样化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene.

CRISPR-based technologies have rapidly enabled the democratization of genome editing in academic institutions through distribution by Addgene over the past decade. Recently, several distribution milestones have been reached, with a collection of >15,000 plasmids deposited by >1,000 laboratories spanning ∼40 countries now shipped 300,000 times to ∼5,000 organizations traversing ∼100 countries. Yet, both deposits of and requests for CRISPR plasmids continue to rise for this disruptive technology. Distribution patterns revealed robust demand for three distinct classes of CRISPR effectors, namely nucleases (e.g., Cas9 and Cas12), modulators (deactivated CRISPR nucleases fused to transcriptional regulators and epigenome modifiers), and chimeric effectors (Cas proteins fused to enzymes carrying out other activities such as deamination, reverse transcription, transposition, and integration). Yearly deposits over the past decade are requested in near-even proportions, reflecting continuous technological development and requests for novel constructs. Though it is unclear whether the slowing rate of requests is inherent to a pandemic operational lag or a transition from emerging to mature technology, it is noteworthy that the relative proportion of requests from plasmids deposited in the previous year remains stable, suggesting robust development of novel tools concurrent with continued adoption of editing, base editing, prime editing, and more. Predictably, most requested plasmids are designed for mammalian genome manipulation, presumably for medical research and human health pursuits, reflecting investments in therapeutic applications. Concurrently, requests for plant and microbial constructs are on the rise, especially in regions of the world more reliant on local agricultural inputs and focused on food and feed applications, illustrating continued diversification of genome editing applications.

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来源期刊
CRISPR Journal
CRISPR Journal Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.30
自引率
2.70%
发文量
76
期刊介绍: In recognition of this extraordinary scientific and technological era, Mary Ann Liebert, Inc., publishers recently announced the creation of The CRISPR Journal -- an international, multidisciplinary peer-reviewed journal publishing outstanding research on the myriad applications and underlying technology of CRISPR. Debuting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a high-profile venue for groundbreaking research, as well as lively and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the Mary Ann Liebert, Inc. portfolio, which includes GEN (Genetic Engineering & Biotechnology News) and more than 80 leading peer-reviewed journals.
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