绿色列表v2.0：自定义CRISPR屏幕的流线型设计的Web应用程序。

IF 4 4区生物学 Q2 GENETICS & HEREDITY

CRISPR Journal Pub Date : 2025-06-01 Epub Date: 2025-05-07 DOI:10.1089/crispr.2025.0023

Esbjörn Henkel, Zhaojun Li, Daniel Uvehag, Bernhard Schmierer, Martin Henkel, Fredrik Wermeling

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引用次数: 0

摘要

定制CRISPR屏幕是快速、假设驱动的发现的强大工具，但它们的设计通常很复杂且耗时。Green Listed v2.0通过设计自定义CRISPR间隔器库的直观工作流程简化了这一过程，并支持所有用户的下游分析，无论他们的计算经验如何。该web应用程序具有用户友好的图形界面，可在https://greenlisted.cmm.se免费访问。2.0版本包含了基于用户反馈实现的对2016年原始版本的重大升级。这包括一个新的基因同义词工具，扩展的库选项，优化的输出列表，性能改进，以及用于合理设计自定义CRISPR筛选基因集的链接脚本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Green Listed v2.0: A Web Application for Streamlined Design of Custom CRISPR Screens.

Custom CRISPR screens are powerful tools for rapid, hypothesis-driven discovery, but their design is often complex and time-consuming. Green Listed v2.0 simplifies this process with an intuitive workflow for designing custom CRISPR spacer libraries and supports downstream analysis for all users, irrespective of their computational experience. The web application features a user-friendly graphical interface freely accessible at https://greenlisted.cmm.se. Version 2.0 includes significant upgrades to the original 2016 version that were implemented based on user feedback. This includes a new gene synonym tool, expanded library options, optimized output lists, performance improvements, and linked scripts for the rational design of custom CRISPR screen gene sets.

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来源期刊

CRISPR Journal Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.30

自引率

2.70%

发文量

期刊介绍： In recognition of this extraordinary scientific and technological era, Mary Ann Liebert, Inc., publishers recently announced the creation of The CRISPR Journal -- an international, multidisciplinary peer-reviewed journal publishing outstanding research on the myriad applications and underlying technology of CRISPR. Debuting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a high-profile venue for groundbreaking research, as well as lively and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the Mary Ann Liebert, Inc. portfolio, which includes GEN (Genetic Engineering & Biotechnology News) and more than 80 leading peer-reviewed journals.