CRISPR Journal最新文献_第2页

Regulatory Support for CRISPR in Ag: A Timely EU-Turn. 对农业中CRISPR的监管支持：及时的欧盟转向。

IF 4 4区生物学

CRISPR Journal Pub Date : 2026-02-01 Epub Date: 2026-02-03 DOI: 10.1177/25731599261421110

Rodolphe Barrangou

引用次数: 0

Efficient Installation of Heterozygous Mutations in Human Pluripotent Stem Cells Using Prime Editing. 利用先导编辑技术在人类多能干细胞中高效安装杂合突变。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-12-01 Epub Date: 2025-09-24 DOI: 10.1177/25731599251380122

Annabelle Suter, Alison Graham, Jia Yi Kuah, Jason Crisologo, Chathuni Gunatilake, Koula Sourris, Michael See, Fernando J Rossello, Mirana Ramialison, Katerina Vlahos, Sara E Howden

{"title":"Efficient Installation of Heterozygous Mutations in Human Pluripotent Stem Cells Using Prime Editing.","authors":"Annabelle Suter, Alison Graham, Jia Yi Kuah, Jason Crisologo, Chathuni Gunatilake, Koula Sourris, Michael See, Fernando J Rossello, Mirana Ramialison, Katerina Vlahos, Sara E Howden","doi":"10.1177/25731599251380122","DOIUrl":"10.1177/25731599251380122","url":null,"abstract":"The utility of human pluripotent stem cells (hPSCs) is greatly enhanced by the ability to introduce precise, site-specific genetic modifications with minimal off-target effects. Although Cas9 endonuclease is an exceptionally efficient gene-editing tool, its propensity for generating biallelic modifications often limits its capacity for introducing heterozygous variants. Here, we use prime editing (PE) to install heterozygous edits in over 10 distinct genetic loci, achieving knock-in efficiencies of up to 40% without the need for subsequent purification or drug selection steps. Moreover, PE enables the precise introduction of heterozygous edits in paralogous genes that are otherwise extremely challenging to achieve using endonuclease-based editing approaches. We also show that PE can be successfully combined with reprogramming to derive heterozygous induced pluripotent stem cell clones directly from human fibroblasts and peripheral blood mononuclear cells. Our findings highlight the utility of PE for generating hPSCs with complex edits and represent a powerful platform for modeling disease-associated dominant mutations and gene-dosage effects in an entirely isogenic context.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":"401-411"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BridgeRNAton: Scandalously Precise DNA Matchmaking by Non-Coding RNAs. BridgeRNAton：通过非编码rna进行惊人精确的DNA配对。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-12-01 DOI: 10.1177/25731599251404830

Lisa Lonetti

引用次数: 0

Cosmopolitan Bioethics Under Pressure: Reflections on the Global Observatory for Genome Editing International Summit. 压力下的世界性生物伦理：对基因组编辑国际峰会全球观察站的思考。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1177/25731599251387607

Nils Schütz

引用次数: 0

A Myostatin (MSTN^-/-) Knockout Buffalo Produced by CRISPR-Cas9 Mediated Genome Editing and Somatic Cell Nuclear Transfer. CRISPR-Cas9介导的基因组编辑和体细胞核转移产生的肌生长抑制素（MSTN-/-）敲除水牛

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-12-01 DOI: 10.1177/25731599251401528

Naresh L Selokar, Priyanka Singh, Bosco Jose, Devika Gautam, Kartikey Patel, Ranjeet Verma, Sacchinandan De, Manoj K Singh, Dheer Singh

{"title":"A Myostatin (MSTN-/-) Knockout Buffalo Produced by CRISPR-Cas9 Mediated Genome Editing and Somatic Cell Nuclear Transfer.","authors":"Naresh L Selokar, Priyanka Singh, Bosco Jose, Devika Gautam, Kartikey Patel, Ranjeet Verma, Sacchinandan De, Manoj K Singh, Dheer Singh","doi":"10.1177/25731599251401528","DOIUrl":"https://doi.org/10.1177/25731599251401528","url":null,"abstract":"CRISPR-Cas9 genome editing offers significant opportunities to improve livestock traits; however, its application in buffalo has been very limited, with no prior reports of live gene-edited animals. Here, we report the successful birth of a buffalo edited in the myostatin (MSTN) gene. To achieve this, five single-guide RNAs (sgRNAs) targeting the buffalo MSTN gene were designed and tested in skin-derived fibroblasts. Among these, sgRNA5 exhibited the highest editing efficiency, approaching ∼50%, as confirmed by T7 Endonuclease I assay, Tracking of Indels by Decomposition, and Inference of CRISPR Edits analyses. Single-cell cloning identified six edited fibroblast clonal populations, including one with a bi-allelic frameshift mutation predicted to severely truncate the MSTN protein. These bi-allelic clonal cells were subsequently used as nuclear donors to produce somatic cell nuclear transfer (SCNT) embryos, which were transferred into recipient buffaloes (n = 15). This effort established three pregnancies and resulted in the birth of one live MSTN knockout buffalo calf. Phenotypically, the calf displayed accelerated growth and increased muscle fiber number and size while maintaining normal meat composition. In conclusion, this study reports the world's first gene-edited buffalo generated through CRISPR-Cas9-mediated genome editing combined with SCNT. These findings provide a proof-of-concept for genome editing in buffalo and demonstrate that MSTN disruption can effectively enhance muscle growth and meat production traits.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"8 6","pages":"436-442"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145716586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas9 Single Nucleotide Editing of Tuberous Sclerosis Complex 2 Gene in Mesenchymal Stem Cells. 间充质干细胞中结节性硬化复合体2基因的CRISPR-Cas9单核苷酸编辑。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-12-01 Epub Date: 2025-08-14 DOI: 10.1177/25731599251367059

Abdallah Salemdawod, Brandon Cooper, Yajie Liang, Piotr Walczak, Hartmut Vatter, Jaroslaw Maciaczyk, Miroslaw Janowski

{"title":"CRISPR-Cas9 Single Nucleotide Editing of Tuberous Sclerosis Complex 2 Gene in Mesenchymal Stem Cells.","authors":"Abdallah Salemdawod, Brandon Cooper, Yajie Liang, Piotr Walczak, Hartmut Vatter, Jaroslaw Maciaczyk, Miroslaw Janowski","doi":"10.1177/25731599251367059","DOIUrl":"10.1177/25731599251367059","url":null,"abstract":"The tuberous sclerosis complex (TSC)2 gene regulates the mammalian target of rapamycin (mTOR) pathway, impacting cell proliferation and growth. The loss-of-function mutations, especially in mesenchymal progenitors, drive the development multiple benign and malignant tumors. TSC2 mutations in certain cancer types, e.g., breast cancer, are also associated with poorer prognosis. The databases of TSC2-mutations report point mutations as the most prevalent. We aimed to test the feasibility of inducing point mutations in mesenchymal stem cells (MSCs), targeting the most frequent point mutations of the TSC2 gene, TSC2. c.1864 C>T (p.Arg622Trp), TSC2. c.1832 G>A (p.Arg611Glu), and TSC2. c.5024 C>T (p.Pro1675Leu) using two delivery methods for CRISPR-Cas9. We report a high editing efficiency of up to 85% inducing TSC2 point mutations in hMSCs using lipofectamine-based transfection. Overall, the high editing efficiency of some TSC2 mutations enables the induction and reversal of mutations in primary hMSCs without needing resource-consuming derivation of cell lines frequently distinct from their primary counterparts.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":"412-425"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Prime Editing in Zebrafish. 优化斑马鱼的Prime编辑。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-12-01 Epub Date: 2025-09-19 DOI: 10.1177/25731599251380500

Rehman Basharat, Gina Rizzo, Josiah D Zoodsma, Lonnie P Wollmuth, Howard I Sirotkin

引用次数: 0

The Lexicon of CRISPR: When Is It Too Much? CRISPR词汇：什么时候太过了？

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-10-01 Epub Date: 2025-09-29 DOI: 10.1177/25731599251385430

Rodolphe Barrangou

引用次数: 0

Possible Reversion of CRISPR-Cas9-Edited Sequences in Octoploid Strawberry. 八倍体草莓中crispr - cas9编辑序列的可能逆转。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-10-01 Epub Date: 2025-08-11 DOI: 10.1177/25731599251361374

Xiangyi Sun, Maofu Li, Hua Wang, Yuan Yang, Yanhui Kang, Pei Sun, Jing Dong, Min Jin, Wanmei Jin

{"title":"Possible Reversion of CRISPR-Cas9-Edited Sequences in Octoploid Strawberry.","authors":"Xiangyi Sun, Maofu Li, Hua Wang, Yuan Yang, Yanhui Kang, Pei Sun, Jing Dong, Min Jin, Wanmei Jin","doi":"10.1177/25731599251361374","DOIUrl":"10.1177/25731599251361374","url":null,"abstract":"Gene editing is more challenging in octoploids due to the presence of multiple copies of each gene. However, the ability to edit genes in these plants would allow editing in commercial varieties. Here, we delivered sequences targeting FaMYB9 into octoploid strawberry \"Honeoye\" and identified several gene-edited lines. Among them, the heterozygous gene-edited line FaMYB9CR-15 had curved and wrinkled leaves at 3 months, whereas leaves of 3-month-old wild-type (WT) strawberry seedlings were elliptical with a smooth surface. At that stage, FaMYB9CR-15 leaves also had large patches of wax. We identified 11,402 differentially expressed genes, divided into four clusters, between WT and FaMYB9CR-15 seedlings at 3 months. Notably, cluster 4 genes-related to nonhomologous end joining, microhomology-mediated end joining repairs, homologous recombination, nucleotide excision repair, and mismatch repair-were more highly expressed in the gene-edited line than in the WT. Surprisingly, by 6 months of age, FaMYB9CR-15 leaves had become smooth with small patches of wax, and expression levels of cluster 4 genes were significantly lower than at 3 months. Over the same period, the percentage of FaMYB9 loci harboring the mutant allele decreased from 70.2% to 43.7%. These findings lead us to conclude that there could be reversion of mutated sequences in octoploid strawberry, emphasizing the challenges of gene editing high-ploidy materials.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":"375-389"},"PeriodicalIF":4.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144823209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A "Bare Hope of A Result": The Second CRISPR Patent Appeal. “结果的一线希望”：第二次CRISPR专利上诉。

IF 4 4区生物学

CRISPR Journal Pub Date : 2025-10-01 Epub Date: 2025-07-17 DOI: 10.1177/25731599251361362

Jacob S Sherkow

引用次数: 0