Plasma Medicine最新文献_第6页

Effect of Plasma Discharge on Epidermal Layer Structure in Pig Skin 等离子体放电对猪表皮层结构的影响

Plasma Medicine Pub Date : 2021-01-01 DOI: 10.1615/plasmamed.2021036925

A. Yahaya, J. Krištof, M. Blajan, Fariha Mustafa, K. Shimizu

引用次数: 0

The Potential Use of a Cold Atmospheric Plasma Jet for Decontamination of Hospital Surfaces. A Pilot Study 低温大气等离子体射流在医院表面净化中的潜在应用。一项初步研究

Plasma Medicine Pub Date : 2021-01-01 DOI: 10.1615/PLASMAMED.2021037267

M. Fallon, Sarah Kennedy, Sharath Kumar, S. Daniels, H. Humphreys

{"title":"The Potential Use of a Cold Atmospheric Plasma Jet for Decontamination of Hospital Surfaces. A Pilot Study","authors":"M. Fallon, Sarah Kennedy, Sharath Kumar, S. Daniels, H. Humphreys","doi":"10.1615/PLASMAMED.2021037267","DOIUrl":"https://doi.org/10.1615/PLASMAMED.2021037267","url":null,"abstract":"Hospital surfaces are a source of potential infection due to contamination by microorganisms such as bacteria and viruses. Bacterial biofilms are of particular importance, due to their persistence in the hospital environment. Cold atmospheric pressure plasma (CAPP) is a novel potential method of hospital surface decontamination. In this study, we aimed to show the potential use of a CAPP jet for hospital decontamination. Bacterial biofilms were grown on common hospital surface materials and treated with an air-driven CAPP jet. Viable bacteria in treated biofilms were measured. Confocal microscopy and staining were used to gather images of CAPP-treated biofilms. The effect of CAPP inactivation was also assessed alongside the use of a detergent. CAPP inactivated clinically relevant bacteria both in planktonic and biofilm structures. A 90 s CAPP treatment led to an average of 82% decrease in Escherichia coli (E. coli) and 70% decrease in methicillin-resistant Staphylococcus aureus (MRSA) biofilm viability grown on polysterene. Confocal microscopy was used to confirm CAPP-induced inactivation of bacterial cells within mature growth on glass. CAPP treatment was also compared to treatment with detergent commonly used in hospitals. It was found that biofilm inactivation was greatest when treated with both detergent and CAPP. When in combination with detergent, viability of E. coli and MRSA biofilms grown on polysterene were decreased by 97% and 94%, respectively. A CAPP jet was shown to inactivate biofilms on hospital surfaces, particularly when used alongside detergent. This may suggest a role for CAPP in hospital decontamination.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Nonthermal Atmospheric Pressure Plasma Jet on Human Dental Pulp Stem Cells 非热大气压等离子体射流对人牙髓干细胞的影响

Plasma Medicine Pub Date : 2021-01-01 DOI: 10.1615/plasmamed.2021040860

Seyedeh Fatemeh Peyro Mousavi, A. Ganjovi, A. Eskandarizadeh, M. Parirokh, A. Derakhshani, A. Saidi, A. Falahat

{"title":"Effects of Nonthermal Atmospheric Pressure Plasma Jet on Human Dental Pulp Stem Cells","authors":"Seyedeh Fatemeh Peyro Mousavi, A. Ganjovi, A. Eskandarizadeh, M. Parirokh, A. Derakhshani, A. Saidi, A. Falahat","doi":"10.1615/plasmamed.2021040860","DOIUrl":"https://doi.org/10.1615/plasmamed.2021040860","url":null,"abstract":"The effects of a nonthermal atmospheric plasma jet with argon and argon/oxygen (Ar/O2) gases on live human dental pulp stem cells (hDPSCs) were studied to verify tooth vitality in the pulp-capping process. Using MTT assay, the surviving hDPSCs after plasma jet exposure for 1, 2, and 3 min were counted. First, for the pulsed plasma jet the frequency varied from 10 to 50 kHz for 5 groups with Ar and 5 groups with Ar/O2 plasma jet. Later, with the alternating current (AC) plasma jet, the voltage was changed between 5.5 and 16.05 kV for 13 groups. The cells in the control group were only exposed to Ar and Ar/O2 gases. To control thermal damage to hDPSCs, optical emission spectroscopy was used. For data analysis, the ANOVA repeated measure was used. There were significant differences between the various frequencies and exposure durations (p < 0.05) for the Ar plasma. For the Ar/O2 gas, there were significant differences between frequencies (p < 0.001). For the AC plasma jet with Ar gas, there were significant differences between voltages (p < 0.001). At the higher pulsed jet frequencies, temperatures increased. Eventually, the suitable durations for root canal disinfection and hDPSC survival with the Ar pulsed jet were 2 and 3 min at 10 and 30 kHz, respectively. Since cold plasma disinfection keeps hDPSCs alive, it can be helpful in promoting tooth survival and durability for pulp capping in clinical treatments.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67349062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold atmospheric plasma treatment: a novel method for diabetes mellitus therapy; a basic study 低温常压等离子体治疗糖尿病的新方法基础研究

Plasma Medicine Pub Date : 2021-01-01 DOI: 10.1615/plasmamed.2021040177

A. Rezaeinezhad, Marjan Mahdavi-Gharavia, Mohammad Talebi-Khoshmehra, H. Mirmiranpour, H. Ghomi

引用次数: 2

Growth Comparison Between Lymphocyte BW5147 T-cells and EL4 T-cells Using Atmospheric Oxygen Plasma Irradiation 大气氧等离子体辐照下淋巴细胞BW5147 t细胞与EL4 t细胞生长的比较

Plasma Medicine Pub Date : 2021-01-01 DOI: 10.1615/plasmamed.2021038540

Sitti Subaedah, Haruka Uematsu, N. Hayashi

引用次数: 0

From Precancers to Skin Rejuvenation-A Review of the Wide Spectrum of Current Applications and Future Possibilities for Plasma Dermatology 从癌前病变到皮肤再生——血浆皮肤病学的广泛应用和未来可能性综述

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2020036898

P. C. Friedman

引用次数: 0

Plasma Jet Effectiveness Alteration in Acute Wound Healing by Binahong (Anredera cordifolia) Extract 滨拿红提取物对急性创面愈合等离子体射流效能的影响

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2021037264

S. Darmawati, N. Nasruddin, P. Kurniasiwi, A. Mukaromah, A. Iswara, G. S. A. Putri, H. Rahayu, E. S. Wahyuningtyas, H. Lutfiyati, A. Kartikadewi, S. Rejeki, T. Ishijima, T. Nakatani, J. Sugama

{"title":"Plasma Jet Effectiveness Alteration in Acute Wound Healing by Binahong (Anredera cordifolia) Extract","authors":"S. Darmawati, N. Nasruddin, P. Kurniasiwi, A. Mukaromah, A. Iswara, G. S. A. Putri, H. Rahayu, E. S. Wahyuningtyas, H. Lutfiyati, A. Kartikadewi, S. Rejeki, T. Ishijima, T. Nakatani, J. Sugama","doi":"10.1615/plasmamed.2021037264","DOIUrl":"https://doi.org/10.1615/plasmamed.2021037264","url":null,"abstract":"An atmospheric pressure plasma jet (APPJ) using medical-grade argon gas as the carrier gas was developed and used to investigate wound healing in small animals combined with Binahong (Anredera cordifolia) leaf extract treatment. The experiment was divided into 4 treatment groups, control (C), plasma jet (P), Binahong leaf extract (B), and Binahong leaf extract followed by plasma jet (PB). Plasma jet treatment for wounds was applied in different styles based on the treatment day. Both P and PB treatments were contact-style for days 0–4 (5-mm distance for 1-min duration) and noncontact style for days 5–13 (20 mm for 3 min). The total period of wound observation was 14 days. Histological evaluation using hematoxylin-eosin (HE) staining was performed on days 7, 11, and 14 to evaluate wound reepithelialization. Meanwhile, the number of neutrophil cells was counted using a hematology analyzer on days 11 and 14, and malondialdehyde (MDA) levels were examined on days 7 and 14. This research revealed that plasma jet successfully improved wound healing; on the other hand, plasma jet treatment preceded by Binahong leaf extract treatment tended to impede wound healing. It is hypothesized that Binahong may reduce plasma jet effectiveness in","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Plasma Treatment Effect on the Paramagnetic Species of Barley Seed Radical's Intensity: An EPR Study 等离子体处理对大麦种子顺磁性自由基强度的影响:EPR研究

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2020036353

P. Attri, Anan Teruki, R. Arita, T. Okumura, Hayate Tanaka, D. Yamashita, Kayo Matsuo, N. Itagaki, K. Kamataki, K. Koga, M. Shiratani, K. Kuchitsu, Yushi Ishibashi

引用次数: 2

Effect of Plasma-Activated Medium and Water on Replication and Extracellular Virions of Herpes Simplex Virus-1 血浆活化介质和水对单纯疱疹病毒1型复制和细胞外病毒粒子的影响

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2020033626

V. Tsvetkov, A. Hinkov, D. Todorov, E. Benova, I. Tsonev, T. Bogdanov, S. Shishkov, K. Shishkova

{"title":"Effect of Plasma-Activated Medium and Water on Replication and Extracellular Virions of Herpes Simplex Virus-1","authors":"V. Tsvetkov, A. Hinkov, D. Todorov, E. Benova, I. Tsonev, T. Bogdanov, S. Shishkov, K. Shishkova","doi":"10.1615/plasmamed.2020033626","DOIUrl":"https://doi.org/10.1615/plasmamed.2020033626","url":null,"abstract":"АBSTRACT: We use a surface-wave sustained discharge (SWD) in argon at atmospheric pressure (using a plasma torch) in these experiments. The plasma torch is sustained using a 2.45-GHz electromagnetic wave with applied microwave powers of 13, 15, and 20 W. At these discharge conditions, the length of the plasma torch outside of the quartz tube is ~1–1.5 cm, and the gas plasma temperature does not exceed 40°C. This allows direct treatment of samples using the active zone of the discharge. In the cytotoxicity study, only two of the experimental settings achieve up to 50% survival of the cell monolayer after adding plasma-treated medium. Examining the effect of the plasma torch treatment media on herpes simplex virus-1 replication, we found that none of the applied experimental assays show significant protection on the cell monolayer. In a study of the virucidal action of a plasma-treated viral suspension diluted with sterile water at a ratio of 1:2 that was treated for 300 s at 13-W wave power, a decrease in the viral sample titer occurred unlike in the 1.67 log10 control. Using optical emission spectroscopy, we found that OH intensity increases at the contact point between plasma and the water surface. Intensity of NO-γ also increases to the contact point with applied power. We also monitored the amount of peroxide radicals in plasma-treated water and nutrient medium in the presence of lucigenin.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1615/plasmamed.2020033626","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cold Atmospheric Pressure Plasma as a Tool to Control the Proliferation of Various Mammalian Cells Including Human Mesenchymal Stem Cells for Regenerative Medicine 低温大气压等离子体作为控制包括人间充质干细胞在内的多种哺乳动物细胞增殖的工具用于再生医学

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2021036029

Jeongyeon Park, K. Song

{"title":"Cold Atmospheric Pressure Plasma as a Tool to Control the Proliferation of Various Mammalian Cells Including Human Mesenchymal Stem Cells for Regenerative Medicine","authors":"Jeongyeon Park, K. Song","doi":"10.1615/plasmamed.2021036029","DOIUrl":"https://doi.org/10.1615/plasmamed.2021036029","url":null,"abstract":"Cell proliferation is one of the most critical processes for development, tissue regeneration, and wound healing, and is tightly linked with cell differentiation and migration. Also, the regulation of cell proliferation is essential for appropriate tissue regeneration and improved culture system, which requires an effective control tool for cell proliferation both in vivo and in vitro. Recently, cold atmospheric pressure plasma (CAP) has been shown to have considerable effects on cell proliferation that have been attributed to the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Many studies on CAP application suggest that CAP can be developed as an efficient tool to activate proliferation in vitro for personalized cell therapies and in vivo for wound healing. In this review, we discuss how CAP has been applied to control proliferation in various mammalian cells and its molecular mechanisms for biomedical applications. In our study, we demonstrated that nitric oxide (NO) from CAP is the major factor for activating the proliferation of human mesenchymal stem cells. When exposed to CAP, many different types of human cells displayed highly increased expression of cytokines and growth factors both in vitro and in vivo, strongly suggesting that common mechanisms and components are involved in CAPinduced cell proliferation. Furthermore, CAP induced epigenetic modifications in human mesenchymal stem cells to boost the expression of cytokines and growth factors, thereby promoting cell proliferation. Further studies are needed to verify that NO of the CAP and epigenetic modifications are the common mechanism of action of CAP in culture and in wounded tissues. In addition, the molecular mechanism of the epigenetic modifications induced by CAP treatment should be investigated to develop CAP as an applicable tool for regenerative medicine and wound healing.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0