低温大气压等离子体作为控制包括人间充质干细胞在内的多种哺乳动物细胞增殖的工具用于再生医学

Q3 Physics and Astronomy

Plasma Medicine Pub Date : 2020-01-01 DOI:10.1615/plasmamed.2021036029

Jeongyeon Park, K. Song

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引用次数: 0

摘要

细胞增殖是发育、组织再生和伤口愈合的关键过程之一，与细胞分化和迁移密切相关。同时，细胞增殖的调控对于组织再生和培养体系的完善至关重要，这就需要一种有效的体内外细胞增殖调控工具。近年来，低温大气压等离子体(CAP)已被证明对细胞增殖有相当大的影响，这归因于活性氧(ROS)和活性氮(RNS)的产生。许多关于CAP应用的研究表明，CAP可以作为一种有效的工具，在体外激活细胞增殖，用于个性化细胞治疗，在体内用于伤口愈合。本文综述了CAP在多种哺乳动物细胞增殖调控中的应用及其生物医学应用的分子机制。在我们的研究中，我们证明了来自CAP的一氧化氮(NO)是激活人间充质干细胞增殖的主要因素。当暴露于CAP时，许多不同类型的人细胞在体外和体内均表现出细胞因子和生长因子的高度表达，这强烈表明CAP诱导的细胞增殖有共同的机制和成分。此外，CAP诱导人间充质干细胞发生表观遗传修饰，提高细胞因子和生长因子的表达，从而促进细胞增殖。在培养和损伤组织中，CAP的NO和表观遗传修饰是CAP的共同作用机制，有待进一步研究证实。此外，还需要进一步研究CAP诱导表观遗传修饰的分子机制，以使CAP成为再生医学和伤口愈合的有效工具。

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Cold Atmospheric Pressure Plasma as a Tool to Control the Proliferation of Various Mammalian Cells Including Human Mesenchymal Stem Cells for Regenerative Medicine

Cell proliferation is one of the most critical processes for development, tissue regeneration, and wound healing, and is tightly linked with cell differentiation and migration. Also, the regulation of cell proliferation is essential for appropriate tissue regeneration and improved culture system, which requires an effective control tool for cell proliferation both in vivo and in vitro. Recently, cold atmospheric pressure plasma (CAP) has been shown to have considerable effects on cell proliferation that have been attributed to the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Many studies on CAP application suggest that CAP can be developed as an efficient tool to activate proliferation in vitro for personalized cell therapies and in vivo for wound healing. In this review, we discuss how CAP has been applied to control proliferation in various mammalian cells and its molecular mechanisms for biomedical applications. In our study, we demonstrated that nitric oxide (NO) from CAP is the major factor for activating the proliferation of human mesenchymal stem cells. When exposed to CAP, many different types of human cells displayed highly increased expression of cytokines and growth factors both in vitro and in vivo, strongly suggesting that common mechanisms and components are involved in CAPinduced cell proliferation. Furthermore, CAP induced epigenetic modifications in human mesenchymal stem cells to boost the expression of cytokines and growth factors, thereby promoting cell proliferation. Further studies are needed to verify that NO of the CAP and epigenetic modifications are the common mechanism of action of CAP in culture and in wounded tissues. In addition, the molecular mechanism of the epigenetic modifications induced by CAP treatment should be investigated to develop CAP as an applicable tool for regenerative medicine and wound healing.

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来源期刊

Plasma Medicine Physics and Astronomy-Physics and Astronomy (all)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Technology has always played an important role in medicine and there are many journals today devoted to medical applications of ionizing radiation, lasers, ultrasound, magnetic resonance and others. Plasma technology is a relative newcomer to the field of medicine. Experimental work conducted at several major universities, research centers and companies around the world over the recent decade demonstrates that plasma can be used in variety of medical applications. It is already widely used surgeries and endoscopic procedures. It has been shown to control properties of cellular and tissue matrices, including biocompatibility of various substrates. Non-thermal plasma has been demonstrated to deactivate dangerous pathogens and to stop bleeding without damaging healthy tissue. It can be used to promote wound healing and to treat cancer. Understanding of various mechanisms by which plasma can interact with living systems, including effects of reactive oxygen species, reactive nitrogen species and charges, has begun to emerge recently. The aim of the Plasma Medicine journal will be to provide a forum where the above topics as well as topics closely related to them can be presented and discussed. Existing journals on plasma science and technology are aimed for audiences with primarily engineering and science background. The field of Plasma Medicine, on the other hand, is highly interdisciplinary. Some of prospective readers and contributors of the Plasma Medicine journal are expected to have background in medicine and biology. Others might be more familiar with plasma science. The goal of the proposed Plasma Medicine journal is to bridge the gap between audiences with such different backgrounds, without sacrificing the quality of the papers be their emphasis on medicine, biology or plasma science and technology.