Plasma Medicine最新文献_第7页

Comparative Study of Cancer Treatment Potential Effects of Tumor-Treating Fields and Cold Atmospheric Plasma 肿瘤治疗场与低温大气等离子体治疗肿瘤潜在效果的比较研究

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2020033045

X. Yao, Isaac Goldstein, Li Lin, J. Sherman, M. Keidar

引用次数: 4

Cold Atmospheric Helium Plasma Induces Apoptosis by Increasing Intracellular Reactive Oxygen and Nitrogen Species 低温大气氦等离子体通过增加细胞内活性氧和活性氮诱导细胞凋亡

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/PLASMAMED.2021036830

Latha Ramireddy, Chih-Ho Lai, B. S. Low, Chuan Li, J. Hsieh, Jyh-Wei Lee, Hui-Yu Wu

{"title":"Cold Atmospheric Helium Plasma Induces Apoptosis by Increasing Intracellular Reactive Oxygen and Nitrogen Species","authors":"Latha Ramireddy, Chih-Ho Lai, B. S. Low, Chuan Li, J. Hsieh, Jyh-Wei Lee, Hui-Yu Wu","doi":"10.1615/PLASMAMED.2021036830","DOIUrl":"https://doi.org/10.1615/PLASMAMED.2021036830","url":null,"abstract":"Cold atmospheric pressure plasma (CAPP) has been proposed as a novel method for anticancer therapy. This field gained much interest in the last decade, with biological applications such as wound healing, bacterial sterilization, and cancer treatment. However, the mechanism at the basis of plasma-cell interaction remains unclear. Here, we studied the effect of helium (He) gas CAPP on oral squamous cell carcinoma (OSCC) in vitro. CAPP treatment was performed under different treatment time conditions: 1, 3, and 5 min. Results showed that CAPP treatment induces cell death in OSCC cells in a dose-dependent manner. He-CAPP also induces cell death and G1 cell cycle arrest associated with the ATM/P53 pathway. Furthermore, CAPP activates the mitochondria-mediated apoptosis pathway by enhancing Bax expression and of the Bcl-2 protein suppression. Hydrogen peroxide (H2O2) generation increased immediately after He plasma treatment but reached basal level after 3 h. Further studies showed that CAPP increases intracellular ROS and RNS and reverts after a long period of plasma treatment. Taken together, these results indicated that He-CAPP induces cell death and cell cycle arrest and activates mitochondria-mediated apoptosis by increasing intracellular reactive oxygen and nitrogen species (ROS and RNS) in OSCC cells. Our study provides deep understanding of He-CAPP’s effect on OSCC cells. We suggest that CAPP could be a potential therapeutic and clinical research tool for oral cancer treatment.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development of Niobium Based Coatings Prepared by Ion-Plasma Vacuum-Arc Deposition 离子等离子体真空电弧沉积铌基涂层的研究进展

Plasma Medicine Pub Date : 2020-01-01 DOI: 10.1615/plasmamed.2020034060

V. Taran, I. Garkusha, O. Tymoshenko, A. Taran, I. O. Misiruk, T. Skoblo, S. Romaniuk, V. Starikov, A. Baturin, G. Nikolaychuk

{"title":"Development of Niobium Based Coatings Prepared by Ion-Plasma Vacuum-Arc Deposition","authors":"V. Taran, I. Garkusha, O. Tymoshenko, A. Taran, I. O. Misiruk, T. Skoblo, S. Romaniuk, V. Starikov, A. Baturin, G. Nikolaychuk","doi":"10.1615/plasmamed.2020034060","DOIUrl":"https://doi.org/10.1615/plasmamed.2020034060","url":null,"abstract":"Comparative studies of niobium carbide and niobium carbonitride coatings deposited on AISI 430 stainless steel have been presented. The NbC and NbCN coatings have been deposited by vacuum-arc evaporation in Bulat-type device by using the pulsed biasing mode with repetition frequency 50 kHz, allowing decreasing the micro-arcs formation. An additional magnetic coil for plasma flow focusing was used, allowing one to enhance deposition rate up to 35 μm/h. The phase composition of the obtained coatings was analyzed via X-ray diffraction. The surface morphology was monitored by scanning electron microscopy; whereas, chemical composition was examined by using energy dispersive X-ray analysis. X-ray fluorescent analysis was used to evaluate the thickness of the coatings. The reflectance R(λ) of the obtained coatings in the wavelength 300–625 nm at normal incidence was measured. The XRD data from NbC coating revealed the existence of the niobium carbide phase with a NaCl-type lattice with fine-crystalline grains ranging from 14 to 16 nm. For the NbCN coating, the two-phase state with c-NbC and hexagonal NbN0.95 phases was monitored. The average grain size for c-NbC phase comprised 16–17 nm; whereas, for NbN0.95 the average grain size was only 1–2 nm, confirming formation of a nanocrystalline structure. Surface nanomechanical behavior under nanoindentation of NbC and NbCN was studied. It was revealed that nanohardness for a NbC coating was varied from 30 to 43 GPa; whereas, for NbCN the data spread comprised 30–48 GPa. It was established that the surface of the grown coatings was very smooth with an extremely low amount of macroparticles.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1615/plasmamed.2020034060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Plasma-Activated Water on Soaking, Germination, and Vigor of Erythrina velutina Seeds 血浆活化水对绒红草种子浸泡、萌发及活力的影响

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/plasmamed.2019031667

C. A. Júnior, F. L. D. Menezes, J. O. Vitoriano, D. L. D. Silva

引用次数: 5

Radiofrequency Field-Induced Radiosensitization Is Not Correlated with Induction of Reactive Oxygen Species 射频场诱导的放射敏化与活性氧的诱导无关

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/plasmamed.2020033041

Angela Chinhengo, A. Serafin, J. Akudugu

引用次数: 0

Comparison of Cellular Sensitivity to a Split Radiation Dose and a Combination of a Single Radiation Dose and Electromagnetic Field Exposure 细胞对分裂辐射剂量和单次辐射剂量与电磁场照射组合的敏感性的比较

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/PLASMAMED.2018027349

Angela Chinhengo, A. Serafin, J. Akudugu

引用次数: 3

Chemistry of Air, N2, and O2 Reverse Vortex Gliding Arc Plasma System 空气、N2和O2反向涡旋滑行电弧等离子体系统的化学性质

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/PLASMAMED.2019029026

Zhengduo Wang, Qiang Chen, Yen Wei, A. Fridman, G. Fridman, H. Ji

引用次数: 1

Radiofrequency Field-Induced Radiosensitization Is Related to Reductions in Metabolic Activity 射频场诱导的放射致敏与代谢活性降低有关

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/plasmamed.2020032850

Angela Chinhengo, A. Serafin, J. Akudugu

{"title":"Radiofrequency Field-Induced Radiosensitization Is Related to Reductions in Metabolic Activity","authors":"Angela Chinhengo, A. Serafin, J. Akudugu","doi":"10.1615/plasmamed.2020032850","DOIUrl":"https://doi.org/10.1615/plasmamed.2020032850","url":null,"abstract":"Although radiofrequency fields (RFFs) have been found to exhibit both radiosensitizing (enhancement of radiation) and radioprotective (mitigation of radiation) effects, mechanisms underlying these phenomena have not been clearly elucidated. Here, we use four human cell lines, namely, MeWo and Be11 (melanomas), DU145 (prostate carcinoma), and L132 (normal lung fibroblasts), to assess the role of RFF modulation of cellular metabolic activity in altering radiosensitivity. We measure radiosensitivity and metabolic activity using colony-forming and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, respectively. Cell lines that are more radiosensitized by RFF exposure show larger reductions in metabolic activity, relative to radiation treatment alone, regardless of whether RFF exposure occurs before or after X-ray irradiation. The finding that surviving cells maintain elevated metabolic activity when treated with a combination of RFFs and X-rays suggests that changes in metabolic activity may be triggered by RFFs to support processes such as DNA repair and alteration of long-term cell survival. Modulation of cellular metabolic activity by RFFs may have important ramifications for moderating ionizing radiation–induced effects. This must be carefully considered if RFFs are to be applied as adjuvants in radiotherapy.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1615/plasmamed.2020032850","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiofrequency Fields Preferentially Enhance In Vitro Cellular Radiosensitivity to Large Fractional Doses in a p53-Dependent Manner 射频场以p53依赖的方式优先增强体外细胞对大分数剂量的放射敏感性

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/plasmamed.2020032818

Angela Chinhengo, A. Serafin, J. Akudugu

{"title":"Radiofrequency Fields Preferentially Enhance In Vitro Cellular Radiosensitivity to Large Fractional Doses in a p53-Dependent Manner","authors":"Angela Chinhengo, A. Serafin, J. Akudugu","doi":"10.1615/plasmamed.2020032818","DOIUrl":"https://doi.org/10.1615/plasmamed.2020032818","url":null,"abstract":"Hypofractionated radiotherapy, which employs large fractions of ionizing radiation, is an effective treatment modality for most superficial cancers, but may result in severe side effects from normal tissue toxicity. It is, therefore, desirable to identify radiation modifying agents that potentiate the tumor inactivating effects of ionizing radiation and thereby lead to a reduction in radiation dose and prevent normal tissue toxicity. This study assessed the effect of radiofrequency fields (RFF), modulated at 100 and 1000 Hz, on the radiosensitivity of four human cell lines: MeWo (melanoma; p53 mutant), Be11 (melanoma; p53 wild-type), DU145 (prostate cancer; p53 mutant), and L132 (normal lung fibroblasts; p53 wild-type), using the colony assay. The magnetic flux densities that were induced in cell cultures ranged from 6.74 to 22.43 μT. The data demonstrate that RFF are more efficient in modulating large fractional doses of X-rays in a frequencyand cell-type-dependent manner. Their effects on radiosensitivity also appear to be linked to p53 status, with cells with mutant p53 being less sensitized than those that are p53 wildtype. These findings suggest that RFF could find application in hypofractionated radiotherapy as adjuvants, and can have a positive impact on the treatment of superficial tumors, and specifically tumors that are p53 wild-type.","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1615/plasmamed.2020032818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimum Power of Low-Temperature Plasma Selectivity for Human Melanoma Cell Treatment 低温等离子体选择性治疗人类黑色素瘤细胞的最佳功率

Plasma Medicine Pub Date : 2019-01-01 DOI: 10.1615/PLASMAMED.2019030124

Sarut Chaisrisawadisuk, D. Boonyawan, Apirag Chuangsuwanit

{"title":"Optimum Power of Low-Temperature Plasma Selectivity for Human Melanoma Cell Treatment","authors":"Sarut Chaisrisawadisuk, D. Boonyawan, Apirag Chuangsuwanit","doi":"10.1615/PLASMAMED.2019030124","DOIUrl":"https://doi.org/10.1615/PLASMAMED.2019030124","url":null,"abstract":"The number of new cases of melanoma is increasing every year. ity of treatment, plasma medicine has been claimed to be a novel adjunctive procedure. We evalu-temperature plasma (LTP) device for selective tumor eradication, comparing between melanoma and normal keratinocyte cell lines. Human melanoma (G-361) and normal keratinocyte (HaCaT) cell lines were subjected to LTP treatment. levels and exposure durations of LTP were compared in both groups. After the treatment, a MTT assay was used to determine cell viability. The physical plasma characteristics of the three powers were also evaluated. It was found that low power (intensity 1 with frequency 10 Hz) was 0.25 watts, while medium power (intensity 5 with frequency 50 Hz) and high power (intensity 10, with frequency 100 Hz) were 0.94 and 3.0 watts, respectively. Higher powers and longer durations of LTP treatment increased the incidence of cell death in both the HaCaT and G-361 cell lines. LTP eliminated slightly more G-361 than HaCaT cells at 10 and 30 seconds of exposure. At 60 seconds or more, LTP showed a decrease of more than 50% in both the HaCaT and G-361 survival. Medium LTP power with 10between HaCaT and G-361 viabilities (77.1% and 66.3%, respectively; P = 0.01). LTP treatment at medium power (intensity 5 with frequency 50 Hz, equal to 0.94 watts) at 10-second duration .","PeriodicalId":53607,"journal":{"name":"Plasma Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1615/PLASMAMED.2019030124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67348654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0