Journal of Biosafety and Biosecurity最新文献

{"title":"Towards an attitude of responsibility for safety in life science education","authors":"Hetty Huijs ,&nbsp;Enrique Asin-Garcia ,&nbsp;Zoë Robaey ,&nbsp;Vitor A.P. Martins dos Santos","doi":"10.1016/j.jobb.2025.10.002","DOIUrl":"10.1016/j.jobb.2025.10.002","url":null,"abstract":"<div><div>The rapid pace of innovation in the life sciences raises new questions about the safety and security of emerging technologies and processes. Therefore, it is important to investigate what is taught about safety and how it is taught during the early stages of scientific training in higher education. In this study, we conducted a gap analysis based on an inventory of learning content covering different dimensions and elements of safety, including understanding and reasoning related to the Safe-by-Design concept. Each topic was evaluated using two three-level scales: one describing how frequently it appeared in the curriculum and another reflecting the depth of learning, from cognitive knowledge to skill and attitude development. Using Wageningen University as a case study, we conducted a series of qualitative interviews with programme directors and lecturers from various life science divisions. Our results show that the technical aspects of safety received the most attention, particularly when teaching about the development phase of innovations. At the same time, skills related to anticipating and managing risks and other responsibility-related elements needed to be strengthened in the curricula. Notably, the frequency with which a safety element is taught was always higher than the extent to which the corresponding skills were developed. This highlights the need to equip students with the skills needed to improve their own judgment of safety in their future careers.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 4","pages":"Pages 159-167"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145571788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adherence to biosafety standard operating procedures – Knowledge, attitudes, and practices of medical laboratory science students at an autonomous university in Davao City, Philippines","authors":"Maria Bea C. Lao ,&nbsp;Yana S. Usop ,&nbsp;Claire Nicole L. Melendez ,&nbsp;Marianne Felicity Bojo ,&nbsp;Fare Valerie Delgado ,&nbsp;Girly Larceña ,&nbsp;Luchie Mae B. Pacabis ,&nbsp;Clyde S. Baltazar ,&nbsp;Rvin John T. Servillon ,&nbsp;Cynthia V. Dayoan ,&nbsp;April Joy D. Parilla ,&nbsp;Alfredo A. Hinay Jr","doi":"10.1016/j.jobb.2025.10.001","DOIUrl":"10.1016/j.jobb.2025.10.001","url":null,"abstract":"<div><div>Laboratory biosafety in the Philippines was given critical attention during the COVID-19 pandemic, which revealed gaps in the established safety practices and the need for strict protocol adherence. Considering this, the present study was conducted to evaluate the biosafety knowledge, attitudes, and practices (KAP) of medical laboratory science students (N = 262) at an autonomous university in Davao City, Philippines, using a validated questionnaire. The results revealed the students’ high attitudes towards biosafety, with strong compliance in using personal protective equipment (PPE) (mean = 4.89 ± 0.55) and aseptic techniques (mean = 4.84 ± 0.57) and following hand hygiene and laboratory protocols (compliance mean = 4.53 ± 0.20; routine disinfection mean = 4.85 ± 0.60). Attitude scores ranged from 3.61 ± 0.10 to 4.90 ± 1.39, indicating a significant emphasis on biosafety (4.90 ± 0.53). Statistically significant differences (<em>p</em> &lt; 0.05) in knowledge and practices were observed across demographic variables, with female students and those in higher year levels demonstrating greater adherence and understanding. In sum, the study findings highlight the effectiveness of current biosafety education and the need for ongoing training to maintain a strong safety culture that can serve as a model for other institutions.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 4","pages":"Pages 151-158"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145420227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WHO revises global framework for monitoring nonpharmaceutical interventions in health emergencies","authors":"","doi":"10.1016/j.jobb.2025.12.001","DOIUrl":"10.1016/j.jobb.2025.12.001","url":null,"abstract":"","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 4","pages":"Page 180"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of commercial disinfectants Virkon®S and Microchem Plus against poliovirus","authors":"Leesa Bruggink,&nbsp;David Bagnara,&nbsp;Yi Nong,&nbsp;Matthew Kaye,&nbsp;Linda Hobday,&nbsp;Jade McKenzie,&nbsp;Ann Cornish,&nbsp;Bruce Thorley,&nbsp;Chuan Kok Lim","doi":"10.1016/j.jobb.2025.08.002","DOIUrl":"10.1016/j.jobb.2025.08.002","url":null,"abstract":"<div><h3>Introduction</h3><div>The WHO Global Action Plan for Poliovirus Containment, 4th edition (GAPIV) describes the necessary conditions for the containment of poliovirus material in poliovirus-essential facilities (PEFs). PEF status is achieved by successfully undergoing the process of certification by the Global Commission for the Certification of the Eradication of Poliomyelitis in compliance with GAPIV requirements. The GAPIV guidelines cover many topics, including waste management, decontamination, disinfection and sterilization. This element of GAPIV requires PEFs to demonstrate in-house validation of decontamination methods with supporting data.</div></div><div><h3>Methods</h3><div>The method used for validation of chemical products for poliovirus decontamination in this study was based on processes outlined in European Standard EN14476, as recommended by the WHO GAPIV guidelines. In this study, the disinfectants 5 % Microchem Plus and 1 % Virkon®S were evaluated for efficacy against high titre poliovirus in solutions with and without high organic load using an endpoint dilution assay. A poliovirus Sabin 1 strain was used as the reference virus with the titre 8.33 log₁₀ CCID₅₀ (50 % cell culture infectious dose) in 0.1 ml.</div></div><div><h3>Results</h3><div>The poliovirus titre after Microchem Plus treatment was calculated as 5.5 (representing a 2.8 CCID₅₀ titre reduction), indicating only partial inactivation. In contrast, poliovirus in solutions with and without high organic load achieved &gt;4 log₁₀ CCID₅₀ titre reduction (≥6.8 and ≥5.8 reduction respectively), after Virkon®S treatment. The Virkon®S treatment results were at the limit of detection of the endpoint dilution assay, indicating complete inactivation, or as near complete that can be demonstrated with the assay. The virus culture results were confirmed by a molecular assay for the detection of enterovirus RNA.</div></div><div><h3>Conclusion</h3><div>Microchem Plus only partially inactivated poliovirus and cannot be relied upon as the sole decontaminant. In contrast, Virkon®S performed well for both high titre poliovirus in solutions with and without high organic load, achieving &gt;4 log₁₀ titre reduction for both, making Virkon®S a suitable disinfectant for poliovirus.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 3","pages":"Pages 114-119"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling and simulation of periodic lockdowns on COVID-19 dynamics using an adaptive SIRV model with daily-varying rates","authors":"Benny Yong ,&nbsp;Arief Anbiya","doi":"10.1016/j.jobb.2025.08.004","DOIUrl":"10.1016/j.jobb.2025.08.004","url":null,"abstract":"<div><div>We introduce a model for analyzing periodic short-term COVID-19 lockdowns featuring short, alternating periods of <em>W</em> working days and <em>L</em> lockdown days with a weekly cycle: <span><math><mrow><mi>W</mi><mo>+</mo><mi>L</mi><mo>=</mo><mn>7</mn></mrow></math></span>. A modified adaptive SIR epidemic model with vaccination (A-SIRV) with a smooth damping function is used to account for periodic suppression of the transmission rate. We use real-world COVID-19 data from Indonesia, United States, Singapore, and Israel to estimate the non-constant transmission and recovery rates using the Method of Variational Imbedding (MVI). The calculated rates reveal that the results of the simulation using A-SIRV models without a damping function approximate the real-world data accurately enough, with low RMSE and MAE values and high values for the coefficient of determination. We also validate the transmission rates with phases of Delta and Omicron variants and obtain agreeable results. We then simulate various scenarios of periodic short-term lockdown using the damped A-SIRV model, finding that implementing five working days and two lockdown days throughout the entire simulation period is sufficient for consistently flattening the number of active cases in the models for Indonesia and United States. However, for the Singapore and Israel models, the use of five working days and two lockdown days only flattens the number of active cases for several years before a blow-up, an extremely high peak of active cases, appears despite the periodic lockdown remaining in place. We then perform additional numerical simulations for Singapore and Israel models and find that the sudden extreme peak can be suppressed by three scenarios: increasing the share of vaccination to the susceptibles, reducing the number of working days per week to three, or using a dynamic policy of five working days per week followed by a policy shift to only two working days per week when sudden extreme peaks start to form. Our study finds that preventive vaccination should be emphasized while allowing partial economic activity during periodic lockdowns.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 3","pages":"Pages 120-136"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145049120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upgraded hands-on BSL-3 training model significantly improves quality of biosafety personnel training","authors":"","doi":"10.1016/j.jobb.2025.09.002","DOIUrl":"10.1016/j.jobb.2025.09.002","url":null,"abstract":"","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 3","pages":"Pages 148-149"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental pathways and drivers of antimicrobial resistance in waterborne Escherichia coli in Pakistan: a one health perspective","authors":"Adnan Khan ,&nbsp;Shahbaz Ahmad Zakki ,&nbsp;Ijaz ul Haq ,&nbsp;Muhammad Shahzad ,&nbsp;Nauman Khan","doi":"10.1016/j.jobb.2025.08.001","DOIUrl":"10.1016/j.jobb.2025.08.001","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance (AMR) is a global health crisis requiring a One Health approach to integrate surveillance across sectors, particularly in water sources, to control its spread. Considering socioeconomic and environmental factors, this study explored <em>Escherichia coli</em> and AMR patterns in water using a One Health approach.</div></div><div><h3>Methodology</h3><div>This cross-sectional study assessed <em>E. coli</em> and AMR in drinking and sewerage water across three districts in Khyber Pakhtunkhwa, Pakistan. Multi-stage random sampling yielded 840 water samples from 420 households, along with socio-demographic and AMR awareness data. Microbiological analyses identified <em>E. coli</em>, CRE, and ESBL. The resistance profiles were determined using the disk diffusion method, and the association between socio-demographics and awareness was analyzed.</div></div><div><h3>Results</h3><div>The prevalence of <em>E. coli</em> in drinking water was 44 %, with 40 % ESBL, 21 % CRE, and 15 % co-occurrence of ESBL and CRE. Swat had the highest <em>E. coli</em> prevalence in sewerage (78 %) and drinking water (58 %), while Bannu had the highest ESBL levels. Tetracycline resistance was widespread (78 % sewerage and 69 % drinking water). The MAR index showed that 20.5 % of the isolates were resistant to six antibiotics, varying by district. Location influenced the contamination level (<em>p</em> = 0.002). Access to health (OR = 0.497), veterinary services (OR = 0.881), and frequent medical advice (OR = 1.557) lowered <em>E. coli</em> contamination. Education (<em>p</em> = 0.002) and locality (<em>p</em> &lt; 0.001) affected AMR knowledge. Animal area cleaning was significantly correlated with AMR awareness.</div></div><div><h3>Conclusion</h3><div>High <em>E. coli</em> and AMR levels in water pose public health risks because they are driven by poor sanitation, limited water access, and low awareness. Better water management, awareness, and antimicrobial control are essential to mitigate AMR spread.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 3","pages":"Pages 140-147"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening national biosafety capabilities and enhancing global biosafety governance","authors":"Chao Shan,&nbsp;Yuan Zhiming","doi":"10.1016/j.jobb.2025.09.001","DOIUrl":"10.1016/j.jobb.2025.09.001","url":null,"abstract":"","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 3","pages":"Pages 137-139"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between TMPRSS2 gene rs2070788 and rs12329760 variants and COVID-19 susceptibility and severity: A study from Jordan","authors":"Sara S.H. Abu Zaid ,&nbsp;Omar F. Khabour ,&nbsp;Sawsan Abuhammad","doi":"10.1016/j.jobb.2025.08.003","DOIUrl":"10.1016/j.jobb.2025.08.003","url":null,"abstract":"<div><div>Symptoms of coronavirus disease of 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), range from asymptomatic to life-threatening, affecting the respiratory system and many other organs, and involved in SARS-CoV-2 pathogenesis, particularly its viral entry, is the enzyme transmembrane protease serine 2 (TMPRSS2). In response, in this study, polymorphisms in the <em>TMPRSS2</em> gene (rs2070788 and rs12329760) were examined for their association with COVID-19 susceptibility and severity in Jordanians. The study participants (n = 861) included 427 individuals who had never contracted COVID-19 (control group) and 434 who had experienced the disease (case group). The case group was subsequently divided into two subgroups: severe (n = 166) and non-severe (n = 268) COVID-19. The results show that rs2070788 is associated with disease susceptibility in all inheritance models (P &lt; 0.05), as well as an association between rs12329760 and COVID-19 disease severity in both the dominant and over-dominant models (P &lt; 0.05). Further, a higher body mass index (BMI) and older age were associated with disease severity (p &lt; 0.05), though females were less likely to develop severe COVID-19 and to be hospitalized. In conclusion, polymorphisms in the <em>TMPRSS2</em> gene may be considered a potential predictor of COVID-19 outcomes in the Jordanian population. The study findings could be used to manage similar viral infections in Jordan in the future.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 3","pages":"Pages 107-113"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientometric analysis of research on deliberate biosecurity threats reveals North-Transatlantic dominance","authors":"Suryesh Kumar Namdeo,&nbsp;Chandan GN","doi":"10.1016/j.jobb.2025.04.002","DOIUrl":"10.1016/j.jobb.2025.04.002","url":null,"abstract":"<div><div>Academic research is crucial to understanding and preparing for the fight against deliberate biological threats; yet, existing efforts have been fragmented, necessitating a more comprehensive examination of the field’s evolution. This study presents a scientometric analysis of deliberate biosecurity threats over the last 20 years, focusing on publication patterns, collaboration dynamics, research priorities, and leading publication venues. We analyzed 791 articles published between 2004 and 2023 using the Web of Science and Scopus databases. Results indicate a notable increase in publications dominated by North-Transatlantic countries, particularly the United States and the United Kingdom, which prioritize such areas as dual-use technology, synthetic biology, and virus-related biosecurity challenges. A comparative analysis of G7 and BRICS collaborations underscores substantial disparities in research output, with the G7 leading in international biosecurity research collaborations. Furthermore, the study reveals limited biosecurity research and collaboration in the Global South. Therefore, we encourage further exploration of biosecurity research to promote inclusive and collaborative global efforts. This study provides valuable insights for researchers and policymakers regarding biosecurity research priorities related to deliberate threats, and it highlights the need to address global disparities.</div></div>","PeriodicalId":52875,"journal":{"name":"Journal of Biosafety and Biosecurity","volume":"7 2","pages":"Pages 69-78"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}