Current Research in Biotechnology最新文献

{"title":"Probiotics improves sepsis-induced acute lung injury in association with intestinal microbiota and pulmonary concentrations of Th17 and Treg","authors":"Meng Zhang ,&nbsp;Tonghua Chen ,&nbsp;Haochen Mai ,&nbsp;Jiwei Zhao ,&nbsp;Xueru Chen ,&nbsp;Phei Er Saw ,&nbsp;Zhengfei Yang","doi":"10.1016/j.crbiot.2024.100238","DOIUrl":"10.1016/j.crbiot.2024.100238","url":null,"abstract":"<div><p>This study aims to assess the immune status of septic acute lung injury (ALI) at various stages and to investigate whether probiotics can mitigate ALI in septic rats. Rats were randomly assigned to sham, caecal ligation and puncture (CLP), and probiotic treatment after CLP (CLP+PT) group. Intestinal contents, bronchoalveolar lavage fluid (BALF), and lung tissue were collected from rats at 6, 24 and 48 h after CLP. Results showed that Th17 level at 24 h in BALF is significantly higher than that at 6 and 48 h. The Treg level increased after CLP and reached its highest point at 48 h. Pathological damage in the alveolar interstitium intensified over time. At 24 h, Th17 percentage of BALF significantly increased in the CLP group while it significantly decreased in the CLP+PT group, and the Treg percentage of BALF at 48 h showed the same alteration trend. Alveolar interstitial pathological damage in the CLP+PT group was reduced at all time points compared to the CLP group. The abundance of Firmicutes was higher and Bacteroidetes lower in the sepsis group compared to the sham group. However, the abundance of these bacteria was similar between the probiotic and pseudo-probiotic groups. Actinomycetes levels differed significantly among the three groups, with the probiotic group exhibiting significantly higher levels. Bifidobacteria levels were significantly higher in the probiotic group compared to the sepsis group. Phenotype prediction indicated a higher proportion of biofilm formation in the probiotic group compared to the sepsis group. In conclusions, significant alterations in Th17 and Treg levels in BALF of septic rats suggest a shift in the distribution of important T lymphocytes in the lungs during sepsis. Probiotics modulated the gut microbiota of septic rats, downregulated the highest value of Th17 and Treg percentage in BAFL, resulting in mitigated lung injury in rats.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100238"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000649/pdfft?md5=b22f5c8a77972b0f8faeaaa5a8a5d20a&pid=1-s2.0-S2590262824000649-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141954588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of GABA (Gamma-aminobutyric acid) supplementation on biomass, pigments, lipid and protein content of Pseudochlorella pringsheimii under salinity stress","authors":"Pooja Bhatnagar ,&nbsp;Prateek Gururani ,&nbsp;Jyoti Rawat ,&nbsp;Krishna Kumar Jaiswal ,&nbsp;Pankaj Gautam ,&nbsp;Manisha Nanda ,&nbsp;P.K. Chauhan ,&nbsp;Mikhail S. Vlaskin ,&nbsp;Vinod Kumar","doi":"10.1016/j.crbiot.2024.100223","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100223","url":null,"abstract":"<div><p>Microalgae synthesize valuable compounds like pigments, proteins, lipids and carbohydrates under stress conditions, which make them an interesting feedstock for different industries. The effect of GABA (Gamma-aminobutyric acid) supplementation under salinity stress conditions was investigated on growth, biomass, pigments, lipid and protein content of <em>Pseudochlorella pringsheimii</em>. Between the varying treatments, 5 g/L NaCl combined with 2.5 mM GABA was observed as the best concentration for stimulating the production of bioactive compounds in microalga <em>P. pringsheimii</em>. The results revealed a significant increase in biomass content by 93.24 %, compared with the control. Moreover, a significant increase was also observed in the production of chlorophyll (chl) a, chlorophyll (chl) b, total carotenoids (car), protein and lipid content by 112.15 %, 84 %, 29.94 %, 23.08 % and 35.63 %, respectively. However, as compared to control and salinity stress alone, the total carbohydrate content was sharply declined by 52.30 % and 101.79 % in GABA supplemented groups. In addition, the presence of fatty acids and different biomolecules were also confirmed by GC–MS and FTIR analysis. The existence of different essential and non-essential amino acids in substantial amounts was validated by UHPLC analysis. Collectively, the present work contributes to a novel strategy for boosting the coproduction of biomass, lipids, proteins and pigments in <em>P. pringsheimii</em> under unfavorable conditions.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100223"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000492/pdfft?md5=02dcfd93316f7fb66470a8dc9aeaf897&pid=1-s2.0-S2590262824000492-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140816924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring metal and metal-oxide nanoparticles for nanosensing and biotic stress management in plant systems","authors":"Vijay Rani Rajpal ,&nbsp;Yashika Dhingra ,&nbsp;Lisha Khungar ,&nbsp;Sahil Mehta ,&nbsp;Tatiana Minkina ,&nbsp;Vishnu D. Rajput ,&nbsp;Azamal Husen","doi":"10.1016/j.crbiot.2024.100219","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100219","url":null,"abstract":"<div><p>Nanotechnology offers promising solutions for climate-resilient agriculture, countering challenges like stagnant yields, emerging pests, and environmental stresses posed by changing global climates. Nanoparticles (NPs) possess unique properties and biological interactions. Metal-based NPs have been tailored for functions like antimicrobial activity, insecticidal properties, and weed inhibition and hold promise for combating biotic stresses and offer the potential for plant pest control, disease detection and management, stress resilience, weed control, and enhancing biomass and crop yield. Metallic NPs repel pests, exhibit larvicidal and ovicidal properties, combat plant pathogens, deliver agrochemicals precisely, and prevent weed growth, eventually boosting agricultural productivity. Numerous NP-based metal and metal oxide nanoproducts, including nanocarriers for nanofertilizers and nanopesticides, nanobiosensors for early pathogen detection, and nanoclays for weed control have flooded the market. Though, mechanistic details of NPs action in mitigating biotic stresses are poorly accounted for, metallic NPs combat pathogens by incurring DNA damage and generating reactive oxygen species (ROS). They fortify plant defense by activation of regulatory factors, induction of antioxidant systems, activation of stress-related genes, and modulation of the metabolic pathways to enhance plant growth. Nevertheless, nanotechnology in agriculture is in its infancy yet, necessitating further research to comprehend its merits and demerits. The potential toxicological effects of NPs underscore the importance of optimizing their dosage to maximize benefits while minimizing negative impacts. Further, redressal of regulatory and safety concerns associated with NPs application in agriculture is essential to ensure their safe and sustainable usage. Clear universal guidelines and standardized testing protocols need to be mandated to uphold their global implementation to transform agriculture.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100219"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000455/pdfft?md5=2d0b0e513db8f205984a8c0c0af91cb5&pid=1-s2.0-S2590262824000455-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140893874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AVPI analogs and conjugates: Molecular docking studies and in vitro biological evaluation","authors":"Maya G. Georgieva ,&nbsp;Silvia G. Mihaylova ,&nbsp;Aneliya A. Balacheva ,&nbsp;Antoaneta Z. Tsvetkova ,&nbsp;Tamara I. Pajpanova ,&nbsp;Nikolay T. Tzvetkov","doi":"10.1016/j.crbiot.2024.100246","DOIUrl":"10.1016/j.crbiot.2024.100246","url":null,"abstract":"<div><p>In recent years, small peptide and non-peptide AVPI-/Smac-mimetics have been developed as IAP antagonists and are in clinical trials to overcome resistance to apoptosis in various cancer types. In this study, we present molecular modeling studies and <em>in vitro</em> biological evaluation of a set of AVPI-mimetics, including parent AVPI, tetrapeptide AVPI-mimetics and AVPI-conjugates.</p><p>Combined molecular modeling studies and HYDE analyses provided valuable information regarding the protein–ligand interactions within the binding site of cIAP1-BIR3 and XIAP-BIR3 domains, showing that the binding part of both domains (cIAP1- and XIAP-BIR3) are formed from 22 amino acid residues, and their active part of 11 AAs. Moreover, 5 amino acids are defined common for both targets, namely Lys299, Gly306, Leu307, Trp310, and Trp323. Based on the observed docking models, six amino acid residues for cIAP1-BIR3 and five amino acids for XIAP-BIR3 are recognized actively involved in the formation of H-bonds with the respective ligand. The amino acid sequence 308 (Arg308 in cIAP1-BIR3, Thr308 in XIAP-BIR3), simultaneously forming two H-hydrogen bonds, seems to plays a key role in improvement of binding affinity.</p><p>Apart from docking results the synthesized set of AVPI-mimetics was tested <em>in vitro</em> using cell biology (MTT assay) and parallel artificial membrane permeability assay (PAMPA). The results showed that the double modification of AVPI <em>via</em> substitution of Pro³ with Hyp³, as well as elongation of AVPI’s C-terminus by its conjugation with RGD-analogs, significantly increase the antiproliferative effects of AVPI-conjugates on all tested cancer cell lines (MDA-MB-231, MCF-7, HepG2 and HT-29 cells) compared to the parent AVPI peptide. SARs analysis defined this modification beneficial for the overall biological activity of the AVPI-mimetics and pointed out AVHypI-AgbGD as the most active conjugate with an IC₅₀ of 348 µM for MDA-MB-231, 457 µM for MCF-7, 399 µM for HepG2, and 578 µM for HT-29 cells. Though the calculated IC₅₀ values were still high, we consider AVHypI-AgbGD peptide as a good basis for further modifications. In addition, PAMPA results showed that substitution of Pro with Hyp improved the BBB permeability of AVHypI peptide compared to its parent molecule.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100246"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000728/pdfft?md5=4e153c7f5b30b9819d64079e3e92313a&pid=1-s2.0-S2590262824000728-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient phytoremediation of Cd-contaminated soils by Tagetes patula L.: Greenhouse experiment, field study and meta-analysis","authors":"Lizhu Yuan ,&nbsp;Xuemei Zhong ,&nbsp;Jianbo Liao ,&nbsp;Ling Zheng ,&nbsp;Xiaowu Huang","doi":"10.1016/j.crbiot.2024.100212","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100212","url":null,"abstract":"<div><p>Applying (hyper)accumulators to remediate Cd-contaminated agricultural soils is vital to safeguard food safety and human health. <em>Tagetes patula</em> L. (Marigold) − an omnipresent hyperaccumulator − has been extensively explored with artificially Cd-contaminated soils. Little is known about its feasibility and potential for remediating naturally Cd-contaminated soils and field applications. In this study, the Cd remediation potential of Marigold was assessed with greenhouse and field studies, with the control groups of <em>Solanum nigrum</em> L. (Black nightshade), <em>Amaranthus Hypochondriacus</em> L. (Amaranth) and <em>Pennisetum purpureum</em> K. Schumach. × <em>P. thyphoideum</em> Rich. (King grass). The results of greenhouse experiment showed that Marigold obtained the highest shoot Cd (4.69 mg·kg⁻¹), Cd uptake amount (93.47 μg·pot⁻¹), translocation factor (TF, 2.80) and bioconcentration factor (BCF, 2.67) while remediating naturally Cd-contaminated soils. Moreover, the field study validated its superior phytoremediation potential of Cd – Marigold achieved 150.80 g·ha⁻¹ while treating a Cd-contaminated farmland (1.72 mg·kg⁻¹). Further, the <em>meta</em>-analysis corroborated Marigold’s strength over other control plants in remediating Cd-contaminated soils, holding the mean effect size of BCF and TF of 1.54 and 0.61, respectively. Taken together, Marigold is promising for the remediation of Cd-contaminated fields. Knowledge gleaned from this study provides an effective approach for the practical phytoremediation of Cd-contaminated soils.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100212"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000388/pdfft?md5=864e198780209cfa564ee1b891c5735c&pid=1-s2.0-S2590262824000388-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling electrokinetic remediation with ryegrass for the synergistic remediation of Cd and C14 alkane co-contaminated soil","authors":"Lizhu Yuan ,&nbsp;Shuhai Guo ,&nbsp;Yang Wu ,&nbsp;Bo Wu ,&nbsp;Fenglian Cheng ,&nbsp;Jinghao Gao ,&nbsp;Xiaowu Huang","doi":"10.1016/j.crbiot.2024.100202","DOIUrl":"10.1016/j.crbiot.2024.100202","url":null,"abstract":"<div><p>Electrokinetic coupled with phytoremediation (EKPR) is an innovative technology to remediate heavy metals or petroleum hydrocarbons polluted soils. However, the feasibility and mechanism of EKPR remediation heavy metals and petroleum hydrocarbons co-contaminated soil are still unknown. This study evaluated the feasibility and potency of coupling EK remediation with <em>Lolium perenne</em> L. (ryegrass) (EKRG) in remediating the cadmium (Cd) and C14 alkane co-contaminated soil. The ryegrass biomass, Cd uptake amount, C14 alkane removal and soil bacterial community were determined for different soil sections of the EKRG treatment. Results indicated that the polarity reversal direct current electric field increased the total Cd uptake amount of ryegrass to 17.95 μg, most likely due to an increase of 41.7 % of the ryegrass shoot biomass. The ryegrass shoots, in the initial anode section of EKRG treatment, acquired an increment in biomass by 82.1 % and Cd uptake amount by 67.1 %, corresponding to 1.02 g and 7.76 μg, respectively. The EKRG treatment boosted the removal of C14 alkane in comparison with the mono-treatment of ryegrass or EK, i.e., increasing by 28.4 % and 36.5 %, respectively. Microbial community analysis suggested that the EKRG treatment promoted the relative abundances of hydrocarbons degrading bacteria, potentially responding to the enhanced removal of C14 alkane. Further, the correlation analysis showed that the removal of C14 alkane was positively correlated with the ryegrass biomass and Cd uptake amount in the EKRG treatment, which again validated that the addition of electric field benefited the remediation of Cd and the removal of C14 alkane. These results together indicated that the electric field assisted phytoremediation would be an effective approach for remediating the Cd and C14 alkane co-contaminated soils.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100202"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000285/pdfft?md5=3d9cdfd6e56be63942453b78183bad43&pid=1-s2.0-S2590262824000285-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in chemical composition and fermentation profile in silages from rehydrated grains of maize hybrids during prolonged air exposure","authors":"Kristina Kljak,&nbsp;Veronika Gunjević,&nbsp;Tea Kavčić,&nbsp;Dora Zurak,&nbsp;Marija Duvnjak,&nbsp;Goran Kiš,&nbsp;Darko Grbeša","doi":"10.1016/j.crbiot.2024.100206","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100206","url":null,"abstract":"<div><p>Most studies investigated the effects of air exposure during the ensiling of whole maize silage and high-moisture maize grains on the chemical composition and fermentation profile, but not of rehydrated grain. The aim of the present study was to investigate the effect of the hybrid and the duration of air exposure on the pH and the content of moisture, main nutrients, fermentation products and nitrogen fractions in silages from three commercial maize hybrids. The grains were rehydrated to 32 % of moisture and ensiled for 63 days with the addition of an inoculant (BIO-SIL®, Dr Pieper Technologie und Produktentwicklung GmbH). After opening, the silages were stored at room temperature for 10 days and samples were taken after 0, 1, 3, 5 and 10 days of air exposure. With increasing duration of air exposure, the content of moisture, lactic and acetic acid and ethanol decreased, while the pH value and the content of water-soluble carbohydrates and ammonia increased. The hybrid affected all the determined properties of the silages except the pH and the ethanol content. There were distinct differences in the contents of moisture, total sugars, water-soluble carbohydrates, lactic, acetic, propionic and isobutyric acids, methanol and ammonia, suggesting that some hybrids may be more susceptible to aerobic spoilage. The majority of the properties remained similar until the fifth day of air exposure, suggesting that the silages of all three hybrids were stable during this period. The only property that increased immediately after air exposure was the content of nitrogen fractions suggesting that proteolysis was occurring, even though pH and lactic acid content remained unchanged. Overall, the results of the study indicate that the behaviour of rehydrated maize silage after exposure to air should be taken into account when selecting maize hybrids.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100206"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000327/pdfft?md5=d02d509710cac9ca886a3ffd5fa3e494&pid=1-s2.0-S2590262824000327-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140345164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From circadian sleep disruption to Neuroprotection: The potential of VIP/PACAP in Alzheimer’s disease treatment","authors":"Artur Galushkin,&nbsp;Illana Gozes","doi":"10.1016/j.crbiot.2024.100254","DOIUrl":"10.1016/j.crbiot.2024.100254","url":null,"abstract":"<div><p>Alzheimer’s Disease (AD) represents a significant neurodegenerative challenge with current therapeutic strategies primarily focused on symptomatic management. This review explores the relationship between disrupted circadian rhythms, AD and the critical involvement of vasoactive intestinal peptide (VIP) and adenylate cyclase-activating polypeptide (PACAP) signaling pathways. These pathways hold promise for new drug development and provide insights into the complex pathogenesis of AD. Enhancement of brain bioavailability through advanced drug delivery systems is proposed and reviewed.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100254"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000807/pdfft?md5=f7e237b1900fb54ad5808871e2f3de13&pid=1-s2.0-S2590262824000807-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142228723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new frontier in neuropharmacology: Recent progress in natural products research for blood–brain barrier crossing","authors":"Ureña-Vacas Isabel ,&nbsp;Aznar de la Riera M. Belén ,&nbsp;Serrano Dolores R ,&nbsp;González-Burgos Elena","doi":"10.1016/j.crbiot.2024.100235","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100235","url":null,"abstract":"<div><p>Neuropharmacology faces challenges due to the intricate nervous system, diverse neurological disorders, and existence of the blood–brain barrier (BBB), which hinder the development of effective treatments. Although the primary function of the BBB is to expel toxins and pathogens, this structure also prevents optimal drug delivery. Natural products, with their chemical diversity and sustainability, have long been recognized as potential neuroprotective compounds, making BBB permeability studies mandatory. Over the last ten years, biotechnological advances in two-dimensional <em>in vitro</em> BBB models (monoculture and co-culture), <em>in vivo</em> imaging techniques, and pharmacokinetic modeling have contributed to expanding our current knowledge. In this study, we have reviewed the BBB crossing of natural products such as different terpenoids, polyphenolic compounds, and alkaloids. The findings, obtained through <em>in vitro</em>, <em>in vivo</em>, and <em>silico</em> methods, revealed moderate to high permeability for many of these natural products. However, other compounds showed not to be able to reach the brain. To better understand the behavior of natural products in humans and improve their ability to pass across the blood-brainier, the development of new three-dimensional and dynamic models of the BBB, new nanosystems complexes for encapsulation or in-depth studies of the transport mechanism are current and future lines of research.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100235"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000613/pdfft?md5=cf81d235ff82a6bac0aa417759e0a697&pid=1-s2.0-S2590262824000613-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible, controlled-release remdesivir-loaded liposomes tackling the telomerase activity of Non-Small cell lung cancer cells: Preparation, characterization, in vitro biological evaluation, and molecular docking analysis","authors":"Hadeer M. Soudan ,&nbsp;Mahmoud E. Soliman ,&nbsp;Ahmed M.R. Fath El-Bab ,&nbsp;Mohamed A. Ghazy ,&nbsp;Sherif F. Hammad","doi":"10.1016/j.crbiot.2024.100256","DOIUrl":"10.1016/j.crbiot.2024.100256","url":null,"abstract":"<div><div>Non-small cell lung cancer (NSCLC) is a global leading cause of cancer mortality. Herein, remdesivir (RDV) was loaded into biocompatible liposomes (RDV-Lips) composed of 1,2-dipalmitoyl-sn‑glycero-3-phosphocholine (DPPC), cholesterol, and polyethylene glycol hexadecyl ether (Brij-58) to enhance its solubility and anticancer efficiency. The study highlighted the possible RDV-induced underlying events, emphasizing its inhibitory potential of telomerase activity through in-silico docking and in vitro studies. RDV-Lips were developed using thin-film hydration and then subjected to physicochemical characterizations. The selected formulations were evaluated for their stability, in vitro release, and in vitro anticancer activity. The size range of RDV-Lips was 83.8–157.9 nm with a polydispersity index (PDI) lower than 0.23 and entrapment exceeded 93%. The cholesterol content of RDV-Lips offered a control point of RDV release, where high and low concentrations exerted slow and fast release patterns, respectively. RDV-Lips showed enhanced anticancer activity and selectivity. They inhibited colony formation, increased lipid peroxidation, induced apoptosis, and inhibited the telomerase activity in a dose-dependent manner. In conclusion, RDV-Lips overcame RDV solubility problems and enhanced its anticancer efficiency. RDV could be a potential therapy against NSCLC via induction of oxidative stress and inhibition of the telomerase activity, which, in turn, restricts unlimited cellular proliferation and apoptosis induction.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100256"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}