Current Research in Biotechnology最新文献

{"title":"Effect of inhibiting PDHα1 gene expression on the metabolism of fatty liver cells","authors":"Xiaoguang Chen,&nbsp;Qiongxia Lv,&nbsp;Haonan Li,&nbsp;Zhe Wang,&nbsp;Yuxin Chang","doi":"10.1016/j.crbiot.2023.100174","DOIUrl":"https://doi.org/10.1016/j.crbiot.2023.100174","url":null,"abstract":"<div><p>PDHα1 gene encodes catalytic subunit PDHE1α of pyruvate dehydrogenase (PDH) complex. Based on previous studies, it is hypothesized that inhibition of PDH activity prevents the entry of glycolytic pyruvate into TCA cycle, while promotes fatty acid oxidation and reduces liver triglyceride (TG) level, thereby alleviating nonalcoholic fatty liver disease (NAFLD). In this study, an in vitro model for NAFLD was established with medical fat emulsion; the most effective siRNA for PDHα1 gene was screened out by qPCR technology; the alterations in metabolism of glucose and lipid, and structure &amp; function of mitochondria in the NAFLD cells were primarily evaluated after transfecting PDHα1 siRNA. As the results showed, after inhibiting the expression of PDHα1 gene, glucose level in culture medium was time-dependently increased, and LDH activity in the cells was moderately elevated after 24 h of transfection and then returned to the normal level after 48 h; intracellular TG level was decreased while LPS activity was increased in a time-dependent manner; no significant change in mitochondrial structure was observed with or without siRNA transfection, and ATP content was obviously reduced after 24 h of transfection, followed by restoration after 48 h. It can be concluded that inhibiting PDHα1 gene in fatty liver cells enhances lipid degradation, and represses the utilization of glucose to an extent, thus reducing TG level without impacting energy generation required for cell survival.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100174"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000564/pdfft?md5=1d838338247b96776cf94a22e4247851&pid=1-s2.0-S2590262823000564-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An extensive evaluation of laser tissue welding and soldering biotechnologies: Recent advancements, progress, and applications","authors":"Yuxin Chen ,&nbsp;Kehong Wang ,&nbsp;Jun Huang ,&nbsp;Xiaopeng Li ,&nbsp;Yunfeng Rui","doi":"10.1016/j.crbiot.2024.100234","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100234","url":null,"abstract":"<div><p>In the rapidly advancing field of modern medicine, despite the availability of sophisticated techniques, suturing remains a fundamental procedure in both surgical and internal medicine operations. However, this traditional method inevitably gives rise to issues such as inflammation, fibrosis, or secondary damage. Laser tissue welding and soldering technologies offer a non-contact approach to surgical suturing with advantages including simplicity of operation, reduced fibrosis tendency, and ensured wound closure strength. Nevertheless, its applications have been attempts at clinical trials but no actual implementation. The development and implementation of this technique have the potential to revolutionize both internal and external surgery by enabling automated suture placement. This holds significant importance for ensuring suture quality and postoperative performance. This article provides a detailed summary and discussion on the current applications of this biotechnologies in various organ systems within the human body, as well as an exploration into the interaction mechanisms between tissues and lasers. Additionally, it presents a comprehensive analysis and discussion on laser types suitable for bio tissue laser soldering. Prospects for addressing tissue fibrosis resulting from laser contactless suturing are also explored. These insights will serve as valuable references and guidance for future advancements in utilizing lasers as viable alternatives in human surgical suturing.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100234"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000601/pdfft?md5=53b938ac0a82295083cb0c2ef88932ee&pid=1-s2.0-S2590262824000601-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141482831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The landscape of neoantigens and its clinical applications: From immunobiology to cancer vaccines","authors":"Chiranjib Chakraborty ,&nbsp;Anirban Majumder ,&nbsp;Manojit Bhattacharya ,&nbsp;Srijan Chatterjee ,&nbsp;Sang-Soo Lee","doi":"10.1016/j.crbiot.2024.100177","DOIUrl":"10.1016/j.crbiot.2024.100177","url":null,"abstract":"<div><p>Since millions of cancer-related deaths and diagnoses exist yearly, malignant tumors are a primary worldwide health concern. A promising method for treating cancer is tumor immunotherapy, which focuses on neoantigens. Neoantigens are tumor-specific antigens expressed on cancer cells due to genetic changes, viral infections, or other biological processes. They serve as excellent immune system targets to identify and attack cancerous cells. Neoantigens are more immunogenic than tumor-associated antigens (TAAs) because they lack central tolerance. Successful clinical trials of neoantigen-based vaccines have raised interest in individualized tumor immunotherapy. Furthermore, neoantigens represent a significant advancement in cancer immunotherapy, offering the potential for personalized and effective tumor treatments. The identification, synthesis, and application of neoantigen-based vaccines hold promise for improving patient outcomes and revolutionizing cancer treatment approaches. This review focuses on the significance of neoantigens in cancer immunotherapy, their classification and identification, the synthesis of neoantigen vaccines, clinical trials and the principles underlying their therapeutic efficacy.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100177"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000030/pdfft?md5=d8ee1d96cf8a053a52c2d34f3f1983ea&pid=1-s2.0-S2590262824000030-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139393385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of (KLAKLAK)–NH2 analogs against pathogenic microbial strains","authors":"Sirine Jaber ,&nbsp;Yana Evstatieva ,&nbsp;Veronica Nemska ,&nbsp;Dilyana Nikolova ,&nbsp;Emilia Naydenova ,&nbsp;Nelly Georgieva ,&nbsp;Dancho Danalev","doi":"10.1016/j.crbiot.2024.100236","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100236","url":null,"abstract":"<div><p>Many microorganisms pose a threat to human health due to the ever-increasing bacterial resistance to conventional drugs. Nowadays, searching for new alternatives to conventional antibiotics to fight bacterial resistance is a main task. Thus, natural molecules such as amino acids and peptides arise as possible solutions to the problem. The antimicrobial activity of targeted compounds was studied by the agar-diffusion method, using the prepared working solutions of the targeted peptides with the corresponding concentrations. The results of the antimicrobial activity against different test pathogens show specificity, as antimicrobial activity against the used test microorganisms was not found in the investigated short-chain synthetic peptides <strong>Si6</strong>, <strong>Si3</strong> and <strong>Si13</strong>. Antimicrobial activity against <em>Bacillus cereus</em>, <em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>, <em>Propionibacterium acnes</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and the yeasts <em>Malassezia furfur</em> and <em>Candida albicans</em> was established for the long-chain synthetic peptides <strong>Si1</strong>, <strong>Si5</strong> and <strong>Si16,</strong> except <strong>Si5</strong> which does not show activity against pathogenic fungal strain <em>C. albicans</em>. The compound <strong>Si16</strong> where natural Leu in (KLAKLAK)₂-NH₂ is replaced by unnatural Nle is the best candidate for medical drug due to the combined antibacterial and antiproliferative effect as well as long hydrolytic stability.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100236"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000625/pdfft?md5=07b1b34ecb7b9a414f290ceda379d2ec&pid=1-s2.0-S2590262824000625-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141594523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted steps for producing biodiesel from microalgae","authors":"Antonio Tabernero,&nbsp;Álvaro González-Garcinuño,&nbsp;Eva Martín del Valle","doi":"10.1016/j.crbiot.2024.100251","DOIUrl":"10.1016/j.crbiot.2024.100251","url":null,"abstract":"<div><p>Biodiesel production from microalgae is considered one of the main candidates to replace conventional fuels. In addition, the use of ultrasound can be crucial to enhance different steps in the industrial production of this biofuel from this type of microorganisms. This review focuses on the potential of ultrasound technology to increase lipid content in microalgae and improve biomass harvesting and lipid extraction, as well as its potential use in oil transesterification. Specifically, the use of ultrasound pulses in the stationary phase of microalgae growth can act as a stimulus to improve lipid content and can oxidise cell walls, improving lipid extraction and subsequent harvesting. Furthermore, if assisted with ultrasound, the reaction time, alcohol/oil molar ratio, separation process, and energy consumption of transesterification can be reduced compared to conventional methods due to the reduction of the interfacial area. Finally, ultrasound technology can be used if some of the previous processes (i.e., in situ transesterification) are coupled to decrease the number of steps in an industrial process. Regarding scale-up, although some ultrasonic reactors working in continuous operation mode have already been proposed, there are still some drawbacks, mainly related to the knowledge of bubble behaviour in different media and their effect on reactions (enzymatic or in situ transesterifications) as well as the energy consumption if ultrasound technology is used in more than one process simultaneously. These facts need to be studied in more detail to introduce this technology in a large-scale process.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100251"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000777/pdfft?md5=914b82cdf3057fb56cb769058368bd7b&pid=1-s2.0-S2590262824000777-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FEEDS, the Food wastE biopEptiDe claSsifier: From microbial genomes and substrates to biopeptides function","authors":"Victor Borin Centurion ,&nbsp;Edoardo Bizzotto ,&nbsp;Stefano Tonini ,&nbsp;Pasquale Filannino ,&nbsp;Raffaella Di Cagno ,&nbsp;Guido Zampieri ,&nbsp;Stefano Campanaro","doi":"10.1016/j.crbiot.2024.100186","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100186","url":null,"abstract":"<div><p>The production of biopeptides from food waste through microbial fermentation faces challenges arising from the diverse proteolytic abilities of microorganisms and substrate variability, impacting both the quality and yield of generated biopeptides. To address these challenges, preliminary in-silico bioinformatics analyses play a crucial role in evaluating suitable substrates and proteases for the fermentation process. However, existing tools lack comprehensive predictive capabilities for relevant proteases, substrate performance assessment, and final biopeptide family characterization. To overcome these limitations, we developed FEEDS (Food wastE biopEptiDe claSsifier), a novel biopeptide prediction and classification tool. FEEDS predicts biopeptide families based on microbial genome protease profiles and substrate composition during proteolysis. The tool also employs a machine learning approach for functional biopeptide classification. Results from testing on 1000 microbial genomes demonstrate the effectiveness of biopeptide classification, particularly in categorizing peptides derived from substrates like <em>Hordeum vulgare</em> and <em>Vitis vinifera</em> seed storage proteins. In addition to biopeptide classification, our study delves into the distinctive protease profiles of bacteria and yeast genomes. Bacterial genomes exhibited 60 to 100 proteases across 40–55 families. Contrastingly, yeast genomes displayed a more evenly distributed pattern with 150 to 160 protease-encoding genes across 60 to 67 families, surpassing bacterial counts. Remarkably, a substantial portion of yeast proteases (∼66 %) was secreted. Moreover, our integration of a machine learning methodology within the FEEDS pipeline proved highly effective, achieving over 80 % accuracy in predicting the function of peptides derived from seed storage proteins. Notably, longer peptide sequences exceeding 20 amino acids consistently displayed a higher probability of correct assignment compared to shorter counterparts.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100186"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000121/pdfft?md5=dc14271b17d72c293fd1596de17ef07f&pid=1-s2.0-S2590262824000121-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139699535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocarriers for inhaled delivery of RNA therapeutics","authors":"Sebastian G. Huayamares,&nbsp;Ryan Zenhausern,&nbsp;David Loughrey","doi":"10.1016/j.crbiot.2024.100200","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100200","url":null,"abstract":"<div><p>RNA therapeutics are promising for the treatment of lung diseases where small molecules and biologics have failed to have much success in the past. While siRNA and mRNA drugs are now FDA-approved for rare diseases in the liver and as vaccines for infectious diseases, they became clinically established only after suitable advances in drug delivery platforms, such as the case of lipid nanoparticles. Localized delivery of RNA therapeutics through nanocarriers optimized for inhaled administration has the potential to address respiratory diseases without toxicity concerns from off-target effects. Here, we review recent clinical and preclinical progress of nanocarriers for inhaled RNA delivery, analyze the properties of nanocarrier-mediated FDA-approved drugs for respiratory diseases, and highlight compelling efficacy endpoints anticipated from ongoing clinical programs. We also present an overview of physiological challenges and opportunities that impact delivery of inhaled RNA therapeutics to the lungs. Finally, we discuss relevant models for preclinical testing, and translational considerations that can be used to help develop future nanocarriers for inhaled administration of RNA therapeutics.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100200"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000261/pdfft?md5=834eb44220d0a5c3c2cfd946d31c9a7a&pid=1-s2.0-S2590262824000261-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140163323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DAS-ELISA-based detection and RT-PCR validation for precise and timely analysis of Citrus tristeza virus infection in Nepalese citrus species","authors":"Bikash Baral","doi":"10.1016/j.crbiot.2024.100221","DOIUrl":"10.1016/j.crbiot.2024.100221","url":null,"abstract":"<div><p>Citrus viral infections pose a substantial threat to the global citrus industry, resulting in multitudinous economic losses annually. Timely and precise identification of citrus viruses offers a promising avenue for proactive insights preemptively before the instigation of extensive infections. The present study was devised to expedite the prompt identification of the presence of <em>Citrus tristeza virus</em> (CTV) within Nepalese citrus species utilizing a Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay (DAS-ELISA), followed by its validation using reverse transcription PCR (RT-PCR). Samplings extracted from plants harvested from 74 out of the 77 administrative districts within Nepal provide substantial evidence of extensive propagation of viral contagion among citrus cultivars across 64 districts (86.48%) of the nation. A comprehensive analysis of the samples obtained from diverse geographical localities revealed that 36 districts (48.64%) exhibit mild infestation, while 9 districts (12.16%) evince moderate infestation. Moreover, samples harvested from 19 districts (25.67%) were highly infected; intriguingly, specimens from 10 districts (13.51%) manifest an absence of viral intrusion. The results have been validated with RT-PCR assay. Given the profound significance of citrus as a pivotal repository of vitamin C and its essential role in conferring advantageous health attributes to humankind, alongside its substantial contribution to the economic paradigm, a meticulous examination is imperative to safeguard these plants against an array of viral infections. Collectively, the DAS-ELISA methodology along with RT-PCR approaches constitutes an exquisitely sensitive and meticulously specific modality, poised to accelerate the expeditious detection of citrus-associated viruses within burgeoning economies like Nepal. This advancement supports timely and precise detection and diagnosis of agriculturally virulent conditions, thereby forestalling the eruption of viral epidemics and concomitantly curbing their financial repercussions.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100221"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000479/pdfft?md5=14d534248ce6dc95c1e5e1764ee7ad4d&pid=1-s2.0-S2590262824000479-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140760766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid droplets dependent or independent cytoprotective activities of unsaturated fatty acids, Lorenzo’s oil and sulfo-N-succinimidyl oleate on 7-ketocholesterol-induced oxidative stress, organelle dysfunction and cell death on 158N and ARPE-19 cells: Cell targets and benefits of sulfo-N-succinimidyl oleate","authors":"Thomas Nury ,&nbsp;Imen Ghzaiel ,&nbsp;Aziz Hichami ,&nbsp;Claudio Caccia ,&nbsp;Valerio Leoni ,&nbsp;Vivien Pires ,&nbsp;Atanas G Atanasov ,&nbsp;Amira Zarrouk ,&nbsp;Gérard Lizard ,&nbsp;Anne Vejux","doi":"10.1016/j.crbiot.2024.100195","DOIUrl":"10.1016/j.crbiot.2024.100195","url":null,"abstract":"<div><p>7-ketocholesterol is a cytotoxic oxysterol which is frequently increased in many chronic inflammatory and age-related diseases. Thus, the inhibition of the toxicity of 7-ketocholesterol is a major challenge to treat these diseases. 158N oligodendrocytes were used to evaluate the cytoprotective effects of lipids: ω-3 and ω-9 fatty acids (α-linolenic acid (C18:3n-3), eicosapentaenoic acid (C20:5n-3), docosahexaenoic acid (C22:6n-3), erucic acid (C22:1n-9) and oleic acid (C18:1n-9)), Lorenzo's oil (a mixture of oleic and erucic acid, 4:1) and sulfo-N-succinimidyl oleate (SSO, a synthetic derivative of oleic acid). On 158N cells, the ability of these molecules to inhibit 7KC-induced oxiapoptophagy (plasma membrane alteration, loss of ΔΨm, peroxisomal dysfunction, reactive oxygen species overproduction, induction of apoptosis and autophagy) were determined. ARPE-19 epithelial retinal cells were also used to evaluate the cytoprotective effect of SSO on 7KC-induced cell death. Unlike ω-3 and ω-9 fatty acids and Lorenzo's oil, sulfo-N-succinimidyl oleate had no cytotoxic effects over a wide range of concentrations. Noteworthy, unlike fatty acids and Lorenzo's oil, the cytoprotective effects of sulfo-N-succinimidyl oleate on 7KC-induced oxiapoptophagy, a caspase-dependent mode of cell death on 158N cells, were not associated with an accumulation of lipid droplets. In addition, on ARPE-19 cells, sulfo-N-succinimidyl oleate prevented 7KC-induced oxidative stress and cell death. These different characteristics of SSO make it possible to envisage its use for therapeutic purposes in diseases where 7-ketocholesterol levels are increased without eventual secondary side effects due to lipid droplets formation.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100195"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000212/pdfft?md5=1e23bfac82851964c3d64c5b12824733&pid=1-s2.0-S2590262824000212-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent trends in nanozyme research and their potential therapeutic applications","authors":"Aparajita Sen ,&nbsp;Jyoti Oswalia ,&nbsp;Sneha Yadav ,&nbsp;Meenakshi Vachher ,&nbsp;Arti Nigam","doi":"10.1016/j.crbiot.2024.100205","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100205","url":null,"abstract":"<div><p>Nanozymes are a class of nanoparticles that can mimic enzyme activity and be used for various applications in modern clinical therapy. It has recently been observed that nanozymes have multi-enzyme mimicking activities, are highly stable, versatile and easily modified. Moreover, they have high catalytic efficiency, high recovery rates, improved substrate specificity and are suitable for mass production. The catalytic mechanisms of nanozymes mainly include catalase, peroxidase, oxidase, hydrolase and superoxide dismutase-like activities, which enable nanozymes to be used as potential therapeutics against a plethora of infectious and lifestyle disorders. Nanozymes have been used as therapeutics against cancer, inflammatory diseases, neurodegenerative and neurological disorders, bacterial, fungal and viral infections, wounds and diseases associated with Reactive Oxygen Species. The purpose of writing this review is to provide a comprehensive compilation of novel research work that has taken place in the last few years regarding the use of nanozymes for therapy. We have compiled the various kinds of nanozymes and elaborated on their anti-tumorigenic, antioxidant, anti-inflammatory, antibacterial, antifungal, antiviral, and neuroprotective roles. Their modes of action and enzymatic targets have also been discussed. The types and mechanisms of synthesis of nanozymes have also been summarized, along with interactions of nanoparticles with nanozymes. Furthermore, strategies to enhance the compatibility between nanoparticles and nanozymes have also been analyzed. Major focus has been laid on therapeutic applications of nanozymes. The challenges and future perspectives of using nanozymes in clinical therapy have also been debated in the later sections.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100205"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000315/pdfft?md5=7a054637928ba074e88ce430c15940e9&pid=1-s2.0-S2590262824000315-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140347075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}