Current Research in Biotechnology最新文献

{"title":"Gut health improvement by locally isolated probiotics and histomorphometric analysis in Wistar rats","authors":"Zuhra Bibi ,&nbsp;Dilara Abbas Bukhari ,&nbsp;Muhammad Qadeer Sarwar ,&nbsp;Arifullah ,&nbsp;Samina Younas ,&nbsp;Tayyab Manzoor ,&nbsp;Abdul Rehman","doi":"10.1016/j.crbiot.2024.100271","DOIUrl":"10.1016/j.crbiot.2024.100271","url":null,"abstract":"<div><div>In the present investigation, lab-isolated probiotics <em>Weisella confusa</em> MZ735961.1, <em>Lactiplantibacillus plantarum</em> MZ707748.1<em>, L. plantarum</em> MZ710117.1<em>,</em> and <em>L. plantarum</em> MZ735961 were used separately and in combinations to evaluate their effect on gut morphology of Wistar rats. Synergistic groups were formed by 1:1 and labeled as G1 (<em>L. plantarum</em> MZ707748.1 and <em>L. plantarum</em> MZ729681.1), G2 (<em>W. confusa</em> MZ735961.1 and <em>L. plantarum</em> MZ727611.1), G3 (<em>L. plantarum</em> MZ729681.1, <em>W. confusa</em> MZ735961.1, and <em>Lactobacillus acidophilus</em> La-14), G4 (all above mentioned probiotics). Rats were gavage-fed with probiotics according to their colony-forming unit (CFU). The experiment was carried out for 35 days. The bacteria were re-isolated from the gut and identified by biochemical tests which confirmed the administration and re-isolation of different <em>Lactobacillus</em> strains from the gut. Molecular characterization was done through 16S rRNA by using universal primers. After sequencing eight <em>Lactobacillus</em> strains were identified. Histopathology of rats’ intestines was done, and different parameters were examined. Villus height, crypt height, crypt width, mucosa, and sub-mucosa of jejunum were significantly (p = 0.00) increased in the G3 synergetic probiotic group compared to 0-day and negative control. However, the villus width showed non-significant (p &gt; 0.05) variations in both genders. Mucosa tunic, muscle tunic, total wall, and crypt depth were significantly increased (p = 0.00) in the G4 group of medial colon. The study concluded that gut morphology improves as probiotics adhere better to the intestinal epithelium, excluding pathogens, reducing inflammation, enhancing nutrient absorption, and stimulating mucosal growth. This results in improved villus structure and gut wall integrity.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100271"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the impact of electrode and membrane modification in stacked microbial fuel cells for wastewater treatment","authors":"Aritro Banerjee,&nbsp;Rajnish Kaur Calay,&nbsp;Somil Thakur,&nbsp;Mohamad Y. Mustafa","doi":"10.1016/j.crbiot.2025.100278","DOIUrl":"10.1016/j.crbiot.2025.100278","url":null,"abstract":"<div><div>This study investigates the efficacy of treating wastewater using microbial fuel cell (MFC) technology to the safe limits for discharge in the environment. It has been demonstrated that MFC directly converts organic matter present in wastewater into energy. The present study uses a cell design based on simple plate geometry, carbon felt electrodes and Nafion117 as proton exchange membrane separating the anode and cathode chambers. The anode was then modified with heat and acid treatment and PEM was treated with PVDF to improve the performance of the cell. Synthetic dairy wastewater with initial COD of 2412 mg/l was used to test the operation of stack consisting of four cells which were hydraulically connected in series. The stack operated with continuous flow of wastewater. COD removal of the feed water was tested in successive cells to achieve the permissible limits for safe discharge of the effluent. COD decreased from 2412 mg/l to 126 mg/l after the fourth cell. For the power output each cell was treated individually. The power density of each cell was directly proportional to the COD of the influent. The power density of the first cell that has the highest COD was measured at 77.9 mW/m², which is two times that for the cell with unmodified anode and membrane. For the first cell COD removal was the highest at 57 % and 2.6 times more than the cell with the unmodified anode and membrane. These results suggest that targeted modifications to the anode and membrane can significantly boost the MFC performance both in terms of COD removal and corresponding power output. Secondly, up to 93.66 % COD removal may be achieved by four cells hydraulically connected in series. The paper offers some insights for stacking options for implementing at scale up of the MFC technology for wastewater treatment plants.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100278"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation","authors":"Gui-yu Li ,&nbsp;Ji-yong Lin","doi":"10.1016/j.crbiot.2024.100270","DOIUrl":"10.1016/j.crbiot.2024.100270","url":null,"abstract":"<div><h3>Objective</h3><div>Hepatocellular carcinoma <strong>(</strong>HCC) is the most common type of liver cancer and is one of the most common global cancers. <em>Curcuma zedoaria (Christm.) Roscoe</em> is a traditional Chinese herb that has been used for thousands of years in China to treat various types of cancer. Curcumol is one of its primary bioactive sesquiterpenes and has been reported to possess antitumor properties; however, the underlying mechanisms in hepatocellular carcinoma (HCC) are largely unknown. The aim of this study was to reveal the mechanism of curcumol treating HCC based on the network pharmacology and experimental verification.</div></div><div><h3>Materials and Methods</h3><div>Targets of HCC and curcumol were identified. The drugs and disease targets were intersected by <em>Venn Diagram.</em> The KEGG pathway enrichment analysis of curcumol treating HCC was analyzed through the R 3.6.1 software. The effects of curcumol on the inhibition of HCC cell line HepG2 growth and its pro-apoptotic activity were evaluated by cell counting kit-8 and flow cytometry. The expression of microRNA-124 (miRNA-124) mRNA was detected by quantitative real-time PCR. HepG2 cells were transfected with a miRNA mimic and inhibitor. The expression of STAT3 and its phosphorylation were induced by IL-6 and detected by western blotting.</div></div><div><h3>Results</h3><div>MicroRNAs in cancer is a significant enrichment signaling pathway for curcumol treating HCC, according to the KEGG pathway analysis. Curcumol effectively inhibited HepG2 cell growth at 50–150 μg/ml, while it had low toxicity to normal LO2 cells. Using flow cytometry, curcumol strongly promoted apoptosis in HepG2 cells and was more potent than the miRNA-124 mimic, whereas the miRNA-124 inhibitor reduced the pro-apoptotic effect of curcumol. Western blotting revealed that curcumol significantly downregulated the overexpression of STAT3 and its phosphorylation in interleukin-6 induced HepG2 cells, whereas an increased level of STAT3 was observed in the miRNA-124 inhibitor transfected cells after curcumol treatment compared to untransfected cells. The level of miRNA-124 was changed up to 5.87-fold by curcumol treatment.</div></div><div><h3>Conclusions</h3><div>The mechanism underlying the effect of curcumol on inhibition and pro-apoptosis of HepG2 cell growth is possibly related to the miRNA-124/STAT3 pathway.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100270"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species","authors":"Naheem Adekilekun Tijani ,&nbsp;Joseph Hokello ,&nbsp;Emmanuel Eilu ,&nbsp;Saheed Adekunle Akinola ,&nbsp;Abdullateef Opeyemi Afolabi ,&nbsp;Ibrahim Ntulume ,&nbsp;Ismail Abiola Adebayo","doi":"10.1016/j.crbiot.2025.100279","DOIUrl":"10.1016/j.crbiot.2025.100279","url":null,"abstract":"<div><div>Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild <em>Termitomyces</em> species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic <em>Candida</em> species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the <em>Candida</em> isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild <em>Termitomyces</em> mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant <em>Candida</em> pathogens.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ranking economic and environmental performance of feedstocks used in bio-based production systems","authors":"Dania Muhieddine Orfali ,&nbsp;Samir Meramo ,&nbsp;Sumesh Sukumara","doi":"10.1016/j.crbiot.2025.100275","DOIUrl":"10.1016/j.crbiot.2025.100275","url":null,"abstract":"<div><div>Biotechnology offers renewable alternatives for producing food, materials, and numerous functional compounds. While rampant human activities are disrupting planets’ geophysical flows, it is urgent to develop sustainable solutions with novel feedstocks and innovative valorization pathways. With the need to reduce greenhouse gas emissions and enhance circularity, new raw materials termed the next-generation feedstocks (<em>NGFs</em>), such as carbon dioxide, methane, methanol, formic acid, and acetic acid, have emerged as potential feedstocks for bio-based processes. So far, no such review exists that compares the performance of conventional, sugar, lignocellulosic, algae-based feedstocks, and <em>NGFs</em>, which biotechnology could upcycle into a wide range of products. In this review, the economic and environmental performances of the feedstocks are analyzed, and quantifications are presented and standardized based on techno-economic analysis and life cycle assessment models. The main parameters for comparison included the geographical location, unit production cost, and environmental impact categories. The results show that the economic and environmental performances are highly variable among the different feedstocks and their processing routes, also depicting evident tradeoffs. Carbon dioxide, sugar cane molasses and glycerol from waste streams are performing better on assessed indicators overall than other potential feedstocks. Nonetheless, this designed data source is the first step for reliable feedstock selection based on sustainability criteria.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100275"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a thermal stress microfluidic platform to screen stability of therapeutic proteins in pharmaceutical formulations","authors":"O. Bortone ,&nbsp;S. Fiorenza ,&nbsp;M. Baldassarre ,&nbsp;N. Falco ,&nbsp;M. Amidi ,&nbsp;T. Markkula ,&nbsp;P.A. Netti ,&nbsp;E. Torino","doi":"10.1016/j.crbiot.2025.100273","DOIUrl":"10.1016/j.crbiot.2025.100273","url":null,"abstract":"<div><div>Therapeutic proteins have great potentialities for the care of a wide spectrum of diseases, for which other small synthetic drugs result ineffective. Due to challenges related to their immunogenicity, the journey of biologics into clinics still faces obstacles. Among the causes of protein immunogenicity, their natural propensity to aggregation is crucial, indeed, to study their stability, pharmaceutical formulations are generally exposed to diverse environmental physicochemical conditions. Traditional approaches to explore protein behavior are effort-demanding, lengthy and expensive, resulting in a limited knowledge of biomolecule stability. There is an urgent need to develop faster and more cost-effective technologies for biological formulation development. In this work, the conceptualization, design and implementation of a modular and automated microfluidic platform to provide thermal stress to highly concentrated and viscous pharmaceutical formulations is presented. The microfluidic platform validity in terms of reliability and comparability to a forced degradation batch-wise stimulation is demonstrated by thermally stimulating and analyzing through SE-HPLC (Size Exclusion – High Performance Liquid Chromatography) different high concentration (&gt; 100 mg/ml) therapeutic nanobody-based formulations. Remarkably, the ranking of the formulations returned by the microfluidic thermal stress platform follows the same trend obtained through well-established industrial in-batch stimulations. Furthermore, data coming from microfluidic stimulations well correlates to outcomes coming from industrial methodologies for storage and accelerated stability studies.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100273"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights from antiaging-related X discussions: A six-year #Longevity hashtag analysis study","authors":"Joanna Bensz ,&nbsp;Dariusz Sołdacki ,&nbsp;Zilin Ma ,&nbsp;Farhan Bin Matin ,&nbsp;Maima Matin ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100280","DOIUrl":"10.1016/j.crbiot.2025.100280","url":null,"abstract":"<div><div>As social media platforms continue to play an increasingly significant role in shaping public discourse and disseminating scientific information, understanding how longevity and aging-related topics are discussed online has become crucial for researchers and healthcare professionals. This study investigates the global discourse on longevity and aging through the analysis of the hashtag #Longevity on the social media platform X (formerly Twitter) over a six-year period from August 1, 2018, to August 1, 2024. A total of 382 032 posts were shared by 109 935 users across 200 countries. The analysis focused on revealing key themes, geographical distribution, sentiment analysis, and the most frequently mentioned supplements and drugs related to longevity. The results show a high level of engagement with the hashtag, primarily driven by users from the United States, followed by the United Kingdom and Canada. Sentiment analysis revealed predominantly positive attitudes towards longevity-related topics, with a slight but statistically significant (p &lt; 0.0001) decline during and after the COVID-19 pandemic. The study identified nicotinamide mononucleotide, rapamycin, and green tea as the most frequently mentioned supplements or drugs in longevity discussions. Notably, there was a significant increase in discussions about niacin derivatives, particularly nicotinamide mononucleotide, during and after the pandemic period. This study highlights the importance of social media as a tool for gauging public interest and sentiment towards scientific topics like longevity, providing valuable insights for researchers, healthcare professionals, and policymakers to enhance science communication and public engagement.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100280"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of substrate type and its constituents on overall performance of microbial fuel cells","authors":"Somil Thakur,&nbsp;Rajnish Kaur Calay,&nbsp;Mohamad Y. Mustafa,&nbsp;Fasil E. Eregno,&nbsp;Ravindra R. Patil","doi":"10.1016/j.crbiot.2025.100272","DOIUrl":"10.1016/j.crbiot.2025.100272","url":null,"abstract":"<div><div>Microbial Fuel Cells (MFC) have emerged as a potential wastewater treatment technology that utilizes metabolic processes of microorganisms present in the wastewater to disintegrate organic substrates and harness direct electricity. This paper reviews the potential of different wastewater types as a suitable substrate for microbial activities in MFCs. Substrate composition (carbon source, nutrient content and inhibitory compounds) directly affects the microbial growth, wastewater treatment potential, electron transfer rate and power harvested. Readily biodegradable substrates such as acetate and glucose promote microbial metabolism and electron transport, thus resulting in enhanced power generation. Substrates such as municipal or agricultural wastewater that constitute both simple and complex organic matter require longer breakdown durations but can provide MFCs with long term operational stability. On the other hand, substrates such as leachate from landfills, mining wastewater etc. are generally unsuitable for regular operations. The paper discusses the challenges such as suitability of various wastewaters, unpredictability of composition, presence of inhibitory chemicals like heavy metals or toxic organics that can subdue the microbial activity and reduce efficiency of a MFC reactor. It aims to identify the relationship between the substrate characteristics and MFC performance in order to select the substrate for achieving optimal output from MFC technology. The suitable substrates that exhibit optimal performance in terms of current and power output, coulombic efficiency, and reduction in chemical oxygen demand are identified.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100272"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic silver nanoparticles produced by Trichoderma reesei inhibit SARS-CoV-2 infection, reduce lung viral load and ameliorate acute pulmonary inflammation","authors":"Marcus V.M.V. Amaral ,&nbsp;Cláudia B. Carraro ,&nbsp;Amanda C.C. Antoniêto ,&nbsp;Mariana N. Costa ,&nbsp;Thais F.C. Fraga-Silva ,&nbsp;Ualter G. Cipriano ,&nbsp;Rodrigo P.F. Abuná ,&nbsp;Tamara S. Rodrigues ,&nbsp;Ronaldo B. Martins ,&nbsp;Andreia M. Luzenti ,&nbsp;Glaucia R. Caruso ,&nbsp;Priscyla D. Marcato ,&nbsp;Vania L.D. Bonato ,&nbsp;Dario S. Zamboni ,&nbsp;Bergman M. Ribeiro ,&nbsp;Sônia N. Báo ,&nbsp;Joao S. da Silva ,&nbsp;Flávio P. Veras ,&nbsp;Roberto N. Silva","doi":"10.1016/j.crbiot.2025.100277","DOIUrl":"10.1016/j.crbiot.2025.100277","url":null,"abstract":"<div><div>The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), posed a significant global health challenge and still demands efforts to develop new therapies. In this study, we investigated the potential of biogenic silver nanoparticles (AgNPs) synthesized by the fungus <em>Trichoderma reesei</em> to combat SARS-CoV-2 infection. In silico studies showed that AgNPs, ranging from 7 nm to 50 nm, have high affinity for spike protein from different variant of SARS-CoV-2. Our findings show that AgNPs effectively do not affect cell viability in Calu-3 cells, inhibit viral infection in Vero-E6 cells and progression of infection <em>in vitro</em>. Additionally, AgNPs impair caspase-1 activation, lactate dehydrogenase release and IL-1β production by human monocytes. Moreover, our study reveals that AgNPs treatment significantly alleviated acute lung injury induced by SARS-CoV-2 infection in Syrian hamsters. This suggests that AgNPs treatment effectively impairs viral replication or propagation within lung tissue, highlighting its potential as an antiviral agent against SARS-CoV-2. Further investigations are warranted to elucidate the underlying mechanisms of action of AgNPs and to assess their safety and efficacy in clinical settings. Nonetheless, our findings offer promising insights into the development of novel therapeutic strategies for combating COVID-19 and reducing its associated morbidity and mortality.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100277"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes","authors":"Maima Matin ,&nbsp;Natalia Ksepka ,&nbsp;Kamil Wysocki ,&nbsp;Michel-Edwar Mickael ,&nbsp;Alpana Pande ,&nbsp;Jarosław Olav Horbańczuk ,&nbsp;Artur Jóźwik ,&nbsp;Olga Adamska ,&nbsp;Artur Stolarczyk ,&nbsp;Laszlo Barna Iantovics ,&nbsp;Yordanka Ilieva ,&nbsp;Pencho Penchev ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100276","DOIUrl":"10.1016/j.crbiot.2025.100276","url":null,"abstract":"<div><div>Curcumin, the major bioactive compound found in turmeric (<em>Curcuma longa</em>) is a subject of intensive research, due to its multiple bioactivities. Previous studies have investigated metabolic effects of curcumin in humans or established animal research models such as mice, but studies with farm animals have been scarce. In this work, we aimed to study the effects of curcumin supplementation in Bulgarian Murrah buffaloes (<em>Bubalus bubalis</em>), on the serum activities of seven important carbohydrate-metabolizing enzymes, β-glucuronidase, α-galactosidase, α-glucosidase, β-glucosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase. Curcumin was supplemented at a dose of 50 g per animal, per day, and serum activities of the studied enzymes were determined at the start (day 0), middle (day 14), and end (day 28) of the supplementation period. A tendency for increased activity of all studied enzymes was observed upon supplementation with curcumin, with the activity levels of five of the enzymes displaying statistically significant upregulation at day 28 (β-glucuronidase, α-galactosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase). These upregulated activities are in general indicative of increased carbohydrates turnover and detoxification processes, and might provide clues for a better understanding of the mechanisms of action of curcumin <em>in vivo</em>, as well as for novel approaches to rationally impact animal or human health and wellbeing through targeted supplementation.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}