Current Research in Biotechnology最新文献

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Systematic comparisons of preparative protocols to generate various types of stone-related crystals for in vitro study of renal calculi 系统比较用于肾结石体外研究的各类结石相关晶体的制备方案
IF 3.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100239
Niracha Koeipudsa, Paleerath Peerapen, Visith Thongboonkerd
{"title":"Systematic comparisons of preparative protocols to generate various types of stone-related crystals for in vitro study of renal calculi","authors":"Niracha Koeipudsa,&nbsp;Paleerath Peerapen,&nbsp;Visith Thongboonkerd","doi":"10.1016/j.crbiot.2024.100239","DOIUrl":"10.1016/j.crbiot.2024.100239","url":null,"abstract":"<div><p><em>In vitro</em> studies of stone-related crystals and crystals-cell interactions have been extensively done to investigate cellular, molecular and pathogenic mechanisms leading to renal calculi. Effective preparation of various types of stone-related crystals is thus crucial for such studies. Nevertheless, various protocols for preparing these stone-related crystals were scatteredly reported without comparative analysis of their efficacies and yields. Herein, we systematically compared our protocols (with the suffix “-Si”) for preparing calcium oxalate (CaOx) monohydrate (COM), CaOx dihydrate (COD), magnesium ammonium phosphate (struvite), uric acid (UA), calcium phosphate dihydrate (brushite), hydroxyapatite (HAP), and calcium carbonate (CaCO<sub>3</sub>) crystals with other protocols published previously. The morphological evaluation revealed that our protocols provided the most homogeneous and most typical monoclinic prismatic, bipyramidal, coffin lid and rectangle shapes of COM, COD, struvite and UA crystals, respectively. There were comparable morphological results for brushite, HAP and CaCO<sub>3</sub> crystals generated by different protocols. Our protocols provided the greatest yield for generating brushite crystals but with lower yields for others. Chemical analysis by Fourier transform infrared (FTIR) spectroscopy revealed comparable results among different protocols to generate each crystal type. In summary, all these protocols can be used to generate each crystal type. But our protocols offer the best quality, in terms of homogeneity and typical shape, for generating COM, COD, struvite and UA crystals.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100239"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000650/pdfft?md5=dd85f9bd8d02cea1de220d5e9b696334&pid=1-s2.0-S2590262824000650-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment 用于分散式污水处理的植被活性污泥法(V-ASP)的生命周期和环境影响评估
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2023.100172
Pu Li , Xiaomeng Hu , Jiajia Yuan , Feiyun Sun , Pengfei Li , Wenyi Dong , Erdeng Du , Mingguo Peng
{"title":"Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment","authors":"Pu Li ,&nbsp;Xiaomeng Hu ,&nbsp;Jiajia Yuan ,&nbsp;Feiyun Sun ,&nbsp;Pengfei Li ,&nbsp;Wenyi Dong ,&nbsp;Erdeng Du ,&nbsp;Mingguo Peng","doi":"10.1016/j.crbiot.2023.100172","DOIUrl":"10.1016/j.crbiot.2023.100172","url":null,"abstract":"<div><p>The integrated Vegetation-Activated Sludge Process (V-ASP) has emerged as a promising solution for decentralized wastewater treatment, offering a unique combination of landscape vegetation with the traditional Anaerobic-Anoxic-Oxic process (AAO). The experiments displayed that V-ASP consistently demonstrated remarkable treatment performance, maintaining stable removal efficiency exceeding 90 % for COD, NH<sub>4</sub><sup>+</sup>-N, and TP. The vegetation growth is helpful for the removal of pollutants and the microbial community in the allocated vegetation root, while the bulk suspended sludge was changed significantly by using PCR test. To comprehensively evaluate the environmental footprint of V-ASP, a Life Cycle Assessment (LCA) was conducted. The mass balance calculation containing wastewater treatment performance, energy consumption, vegetation growth behavior, greenhouse gas (GHG) emissions, treated water, and sludge discharge was carried out to establish the life cycle inventories (LCI). SimaPro 9.0 software and the ReCiPe (H) midpoint impact assessment method were employed, which revealed that the V-ASP system boasts low GHG emissions and freshwater eutrophication potential compared to the traditional AAO process. In essence, this study provides a comprehensive understanding of the V-ASP system, especially the environmental impacts, guiding its potential for sustainable decentralized sewage treatment applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100172"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000540/pdfft?md5=b199791f2cdc6d6f47b7448ee381f95e&pid=1-s2.0-S2590262823000540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green extract surface-coated electrospun cellulose nanofibers as an efficient, reversible, and reusable smart colorimetric sensor for real-time monitoring of chicken freshness 绿色提取物表面涂层电纺纤维素纳米纤维作为一种高效、可逆、可重复使用的智能比色传感器,用于实时监测鸡肉新鲜度
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100192
Muhammad Asim Iqbal , Sabeen Gohar , Yoshinori Yabuta , Liang Fanghua , Wang Ling , Muhammad Farooq , Muhammad Nauman Sarwar , Gopiraman Mayakrishnan , Ick Soo Kim
{"title":"Green extract surface-coated electrospun cellulose nanofibers as an efficient, reversible, and reusable smart colorimetric sensor for real-time monitoring of chicken freshness","authors":"Muhammad Asim Iqbal ,&nbsp;Sabeen Gohar ,&nbsp;Yoshinori Yabuta ,&nbsp;Liang Fanghua ,&nbsp;Wang Ling ,&nbsp;Muhammad Farooq ,&nbsp;Muhammad Nauman Sarwar ,&nbsp;Gopiraman Mayakrishnan ,&nbsp;Ick Soo Kim","doi":"10.1016/j.crbiot.2024.100192","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100192","url":null,"abstract":"<div><p>Smart colorimetric sensor for the naked-eye detection of food freshness is considered as the most attractive tool in food safety. Herein, we prepared curcumin (CUR) coated electrospun regenerated cellulose nanofiber (RCA) composites (CUR-Cot), to monitor the real-time spoilage of raw chicken. The physicochemical properties of the CUR-Cot sensor were completely studied. The surface modification, morphology, crystalline nature, and thermal stability of CUR-Cot were investigated by using various spectral, analytical, and microscopic techniques. Based on the results, the successful coating of CUR on the surface of RCA was confirmed. Interestingly, the CUR-Cot showed a significant change in total color difference value (ΔE, 0 days − 0.0–14.93, after 1 day – 14.93–23.64, after 2 days – 23.64–44.78, after 3 days- 44.78–55.22, and after 4 days – 55.22–60.96, detectable by the naked eye) in the real-time monitoring for chicken freshness. In addition, the present CUR-Cot smart colorimetric sensor is reversible with change in pH, and the reversed sensor can be reused. Furthermore, the hydrophobic nature of the CUR-Cot was confirmed by water contact angle analysis (WCA, contact angle of 90 ± 3.00), which increases its application and adaptability. Good antibacterial, barrier, and optical properties of the present CUR-Cot were also found. Overall, the results confirm that the usage of CUR-Cot sensor can be highly efficient, user-friendly, and non-destructive for the real-time monitoring of chicken freshness.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100192"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000182/pdfft?md5=273c453ff8f94e676def0bd2a303fe31&pid=1-s2.0-S2590262824000182-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140042235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of a prognostic model based on cuproptosis and ferroptosis-related genes in patients with head and neck squamous cell carcinoma 根据头颈部鳞状细胞癌患者的杯突症和铁突症相关基因确定预后模型
IF 3.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100253
Yingjie Wu , Tao Zhou , Juanjuan Hu , Lisha Wu , Yao Hu , Liuqing Zhou
{"title":"Identification of a prognostic model based on cuproptosis and ferroptosis-related genes in patients with head and neck squamous cell carcinoma","authors":"Yingjie Wu ,&nbsp;Tao Zhou ,&nbsp;Juanjuan Hu ,&nbsp;Lisha Wu ,&nbsp;Yao Hu ,&nbsp;Liuqing Zhou","doi":"10.1016/j.crbiot.2024.100253","DOIUrl":"10.1016/j.crbiot.2024.100253","url":null,"abstract":"<div><h3>Background</h3><p>Head and neck squamous cell carcinoma (HNSCC) is a common invasive malignant tumor that lacks powerful predictive or prognostic biomarkers. Ferroptosis and cuproptosis are two new forms of programmed cell death. Our study was aimed at constructing a prognostic model with a combination of cuproptosis and ferroptosis-related genes (CFRGs) for the early clinical detection of HNSCC.</p></div><div><h3>Methods</h3><p>We obtained the information of CFRGs, including the RNASeq data and corresponding clinical data in HNSCC patients from the TCGA and GEO databases. We assessed 28 CFRGs, and analyzed the relationship between those genes and their clinical features and prognosis of HNSCC. The consensus cluster analysis was employed to generate three CFRGclusters. Then, we investigated the association of molecular patterns and prognostic significance in these subtypes. The clinical indicators of the prognosis-related genes were identified and prognostic CFRG_score were constructed. We then built a predictive nomogram with confirmed consistency and reliability by calibration curve analysis. At last, we verified the expression of CFRGs in HNSCC tissues by qRT-PCR and immunohistochemical results.</p></div><div><h3>Results</h3><p>The DEGs were different between the normal and HNSCC tumor tissues and we screened out 28 CFRGs related to the prognosis in HNSCC. Associations between the clinical information and prognosis were found in the molecular subtypes related to prognosis. We utilized enrichment analysis of the differential genes and showed that those DEGs were mostly enriched in the biological processes associated with the pathways of neurodegeneration-multiple diseases, Alzheimer disease, Prion disease, Parkinson disease and Amyotrophic lateral sclerosis. CFRG_score was established to predict the survival of HNSCC patients and found that higher CFRG_score suggested favorable OS for patients, indicating the prediction of better prognosis. Moreover, we created highly reliable nomogram which could predict well for the expected prognosis. In addition, we confirmed that the expression of EGFR, VEGFA, HSPA5, SLC3A2, CAV1 and CD44 were consistent with qRT-PCR and immunohistochemical analysis in HNSCC tissues by qRT-PCR.</p></div><div><h3>Conclusions</h3><p>This prognostic model based on prognostic differential CFRG_score is strongly related to clinical characteristics, prognosis, and therapy in HNSCC patients and could be used as a promising tool which is dedicated to guiding the treatment of HNSCC.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100253"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000790/pdfft?md5=3b344592f4a8895e4773a2d0fd9babcf&pid=1-s2.0-S2590262824000790-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review: Anti-obesity drug discovery from natural plant metabolites and endogenous peptides 综述:从天然植物代谢物和内源性肽中发现抗肥胖药物
IF 3.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100259
Xiaomu Zhu , Dongdong Wang , Atanas G. Atanasov
{"title":"A review: Anti-obesity drug discovery from natural plant metabolites and endogenous peptides","authors":"Xiaomu Zhu ,&nbsp;Dongdong Wang ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2024.100259","DOIUrl":"10.1016/j.crbiot.2024.100259","url":null,"abstract":"<div><div>Obesity is a condition of abnormal or excessive body fat accumulation. It is a chronic and degenerative disease that is a global health problem and causes large societal and economic burdens. However, until now, long-term pharmacotherapies to lower body weight to normal along with suitable tolerability and fewer side effects have remained a challenge. Natural products from plants are a promising source for new lead structures for drug discovery. Additionally, endogenous natural peptides have attracted increasing attention for fighting obesity recently. In this review, we first summarize recent findings on promising signaling/targets for managing body weight, including inhibition of appetite, nutrient absorption and energy expenditure. We also discuss pure natural compounds (such as betulinic acid) and endogenous peptides (such as GLP1, amylin, GIP, ghrelin) and their derivatives (such as orlistat) with anti-obesity effects.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100259"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating the anti-inflammatory and anti-arthritis effects of fucoidan from a brown seaweed 研究褐藻中褐藻糖胶的抗炎和抗关节炎作用
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100220
Preethy P. Raj , Rajesh Kanna Gopal , Elumalai Sanniyasi
{"title":"Investigating the anti-inflammatory and anti-arthritis effects of fucoidan from a brown seaweed","authors":"Preethy P. Raj ,&nbsp;Rajesh Kanna Gopal ,&nbsp;Elumalai Sanniyasi","doi":"10.1016/j.crbiot.2024.100220","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100220","url":null,"abstract":"<div><p>Severe inflammation in joints caused by the detrimental effects of the immune system is termed Rheumatoid arthritis. The unconstrained proliferation of immune cells and pro-inflammatory cytokines deteriorates Synovium which secretes synovial fluid to lubricate joints and cartilage. Non-steroidal anti-inflammatory drugs (NSAIDs) are the only therapeutics for treating rheumatoid arthritis, and long-term intake causes serious side effects on the organs. Fucoidan, a sulfated polysaccharide found on the cell walls of brown algae shows bioactive potential. In our study, fucoidan was extracted from <em>Padina pavonica</em> (PD), <em>Stoechospermum marginatum</em> (StM), <em>Spatolossum macrodontum</em> (SpM), <em>Dictyota bartayresiana</em> (DD), and <em>Turbinaria decurrens</em> (TD) and evaluated for anti-inflammatory and anti-arthritis activities. Fucoidan was extracted and evaluated for anti-inflammatory activity <em>in vitro</em> using RAW 264.7 macrophage cell lines, followed by <em>in vivo</em> anti-arthritis activity on Wistar male rats. Nitric oxide suppression was comparatively high in fucoidan from TD (IC<sub>50</sub> − 12.93 µg/mL). Purified fucoidan from TD, significantly reduced inflammation, size of paw edema, downregulated proinflammatory cytokines (IL-6, IL-1β, TNF-α), and upregulated anti-inflammatory cytokine (IL10) in CFA-induced arthritis in Wistar male rats. Biochemical parameters like SOD, CAT, GSH, GPX, and GST and haematological parameters like total-protein, albumin, haemoglobin, and RBC were upregulated, and other parameters like urea, uric acid, creatinine, bilirubin, SGOT, SGPT, ALP, WBC, ESR, RF, and CRP were downregulated. Histopathology of the liver, kidney, and ankle joints reveals that fucoidan intake restrained inflammation and tissue damage. Therefore, fucoidan extracted from TD is a potential candidate for the treatment of rheumatoid arthritis.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100220"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000467/pdfft?md5=e3746c4e2d04e126ed16f7ed27d36772&pid=1-s2.0-S2590262824000467-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection of aptamer for creatine Kinase-MM as a marker for muscular dystrophy and myocardial infarction 选择肌酸激酶-MM 的适配体作为肌肉萎缩症和心肌梗塞的标记物
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100229
Albert M Liao , Shailaja Agrawal , James Cory Benson , G. Thomas Caltagirone
{"title":"Selection of aptamer for creatine Kinase-MM as a marker for muscular dystrophy and myocardial infarction","authors":"Albert M Liao ,&nbsp;Shailaja Agrawal ,&nbsp;James Cory Benson ,&nbsp;G. Thomas Caltagirone","doi":"10.1016/j.crbiot.2024.100229","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100229","url":null,"abstract":"<div><p>Creatine kinase-MM (CK-MM) is a relatively muscle-specific enzyme with a plasma half-life of approximately 2 h. Total creatine kinase elevation is evident in several conditions associated with acute muscle injury, severe muscular exertion, and myocardial infarction. The presence of a large amount of the CK-MM enzyme in blood serum is a biomarker of muscular injuries and cardiac assault. In this study, we developed a structure-switching aptamer that can be immobilized on a sensor to detect CK-MM. CK-BB was used as a counter-target to ensure the specific targeting of CK-MM. Melting-Off SELEX was employed to develop aptamers exhibiting significant structural changes on binding. The selected aptamer shows a high affinity towards CK-MM with a K<sub>d</sub> value of 14.7 nM.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100229"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000558/pdfft?md5=f3b0eaa258043b204aeb1269ea2335fb&pid=1-s2.0-S2590262824000558-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141240229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source 以糖果工业的淀粉残渣为碳源,利用地中海卤虫生产类胡萝卜素
IF 3.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100265
Micaela Giani , Carmen Pire , Rosa María Martínez-Espinosa
{"title":"Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source","authors":"Micaela Giani ,&nbsp;Carmen Pire ,&nbsp;Rosa María Martínez-Espinosa","doi":"10.1016/j.crbiot.2024.100265","DOIUrl":"10.1016/j.crbiot.2024.100265","url":null,"abstract":"<div><div>Carotenoids are pigments attracting the attention of several industries due to their antioxidant, biological and coloring properties. Low-cost substrates, such as agro-industrial wastes, are being investigated as a viable option to reduce microbial production costs in processes in which microorganisms such as haloarchaea are used as cell factories to produce marketed compounds like carotenoids. They can grow on various agro-industrial wastes and produce the C<sub>50</sub> carotenoid bacterioruberin (BR), which is an extraordinary antioxidant compound with anticancer properties. In this study, the haloarchaeon <em>Haloferax mediterranei</em> is grown in the presence of starch residues from the<!--> <!-->candy industry to induce the production of carotenoids. Cells grew successfully with this industrial waste (max. O.D. 600 nm = 27.75 ± 0.09). Biomass production increased in the presence of higher quantities of starch up to 17.3 ± 0.2 mg/ml of cell culture. The maximum BR concentration was 97.39 ± 1.86 µg/ml. The total amount of BRs synthesized increased when cells grew with increasing concentrations of the industrial starch. The relative percentages of all-<em>trans</em>-BR, 5<em>-cis</em>-BR and a double isomeric BR rose, whereas 9-<em>cis</em>-BR and 13-<em>cis</em>-BR levels decreased.</div><div>Herein, haloarchaeal growth and carotenoid production can be enhanced using industrial waste products as the starch residues selected for this experiment which were provided by a candy company.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100265"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology 利用农杆菌介导的转化,通过合成生物学对具有生物技术价值的真菌瓦氏曲霉进行基因改造
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100178
Carolina Ropero-Pérez, Paloma Manzanares, Jose F. Marcos, Sandra Garrigues
{"title":"Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology","authors":"Carolina Ropero-Pérez,&nbsp;Paloma Manzanares,&nbsp;Jose F. Marcos,&nbsp;Sandra Garrigues","doi":"10.1016/j.crbiot.2024.100178","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100178","url":null,"abstract":"<div><p>In the last years, many research efforts have been applied for the development of filamentous fungi as hosts for heterologous protein production. <em>Aspergillus vadensis</em> CBS 113365, a close relative of the industrial workhorse <em>Aspergillus niger</em>, has been suggested as a more suitable cell factory as it does not acidify the culture medium and produces very low levels of secreted proteases. Therefore, efficient methods and tools that allow the genetic manipulation and exploitation of this biotechnologically relevant fungus are needed. To date, only protoplast-mediated transformation and classical cloning strategies have been implemented for <em>A. vadensis</em> genetic modification, which decreases the exploitation capacity of this fungus at the industrial level. In this study, we have adapted and implemented an <em>Agrobacterium tumefaciens-</em>mediated transformation protocol for <em>A. vadensis</em> for the first time, and applied the FungalBraid system to genetically modify this species by means of synthetic biology. As proof of concept, we have successfully complemented and fluorescently labelled a uridine auxotrophic <em>A. vadensis pyrA<sup>-</sup></em> strain and generated <em>A. vadensis</em> mutants carrying the <em>Penicillium expansum</em>-based expression cassette for the heterologous production of the antifungal protein PeAfpA from <em>P. expansum</em>. Even though we have yet to find the conditions that trigger PeAfpA production in this species, the implementation of the ATMT method reported here, along with the application of the FungalBraid system, will greatly aid in this task and will facilitate the exploitation of <em>A. vadensis</em> as a fungal workhorse for protein production for multiple biotechnological applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100178"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000042/pdfft?md5=0a49fe4461640e9825957bfc2ee33df5&pid=1-s2.0-S2590262824000042-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139487663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Light emitting diode (LED) lights for the improvement of plant performance and production: A comprehensive review 发光二极管 (LED) 灯用于提高工厂绩效和产量:全面回顾
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2024.100184
Saikat Sena , Soni Kumari , Vijay Kumar , Azamal Husen
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