Current Research in Biotechnology最新文献

{"title":"Effects of physical properties on the heavy metal adsorption of biochar via a robust approach","authors":"Mahran Al-Zyoud ,&nbsp;Salama A. Mostafa ,&nbsp;Ibrahim Khersan ,&nbsp;Gowrishankar J ,&nbsp;Prabhat Kumar Sahu ,&nbsp;Siya Singla ,&nbsp;Sardor Sabirov ,&nbsp;Islom. Khudayberganov ,&nbsp;Samim Sherzod","doi":"10.1016/j.crbiot.2026.100367","DOIUrl":"10.1016/j.crbiot.2026.100367","url":null,"abstract":"<div><div>Heavy‑metal contamination of soils and aqueous environments poses critical ecological and health risks, necessitating efficient sorbents for remediation. This study addresses the problem of unpredictable adsorption behavior of biochar by developing a comprehensive machine‑learning approach that relates its physicochemical attributes to metal‑uptake efficiency. A robust dataset of 380 experiments encompassing diverse biomass origins and preparation conditions was assembled to quantify this relationship using descriptors including elemental ratios, pH, cation‑exchange capacity (CEC), surface area, and structural charge. Eight algorithms (Decision Tree, AdaBoost, Random Forest, K‑Nearest Neighbor, Ensemble Learning, Convolutional Neural Network, Support Vector Regression, and Multilayer Perceptron) were evaluated through 5‑fold cross‑validation and optimized by hyperparameter tuning. Statistical indicators (R², MSE, AARE%) and graphical diagnostics confirmed the CNN model as the most reliable predictor (R² = 0.991, MSE = 0.00148), capturing nonlinear physicochemical patterns with minimal overfitting. SHAP interpretation revealed C₀ and CEC as dominant determinants, while surface pH exerted inverse influence on adsorption. The hierarchical feature effects emphasize charge‑controlled and diffusion‑dependent mechanisms rather than morphological properties. The approach provides interpretable, transferable insight into how compositional and activation parameters govern heavy‑metal retention by biochars under varying conditions. Hence, the developed predictive framework not only advances modeling precision but also supports rational design of tailored biochars for environmental detoxification applications.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100367"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyapatite for food packaging applications: translating evidence from biomedical and environmental research","authors":"Qurratu Aini Alya Adzkia ,&nbsp;Atiek Rostika Noviyanti ,&nbsp;Yana Cahyana ,&nbsp;Yaya Rukayadi","doi":"10.1016/j.crbiot.2026.100370","DOIUrl":"10.1016/j.crbiot.2026.100370","url":null,"abstract":"<div><div>The increasing demand for sustainable, functional food packaging has heightened interest in bio-based additives that can enhance material performance while maintaining consumer safety. Hydroxyapatite (HA), extensively studied in biomedical and environmental contexts for its physicochemical stability, ion-exchange capacity, and biocompatibility, has recently been proposed as a prospective additive for food packaging systems. This review critically examines the feasibility of translating insights from biomedical and environmental studies to packaging applications by systematically analyzing peer-reviewed literature published between 2021 and 2025 following PRISMA guidelines. The synthesized evidence indicates that HA incorporation, typically at low filler loadings, has been reported to improve tensile strength and thermal stability, with polymer permeability improvements of up to several tens of percent observed in previous studies. Environmental studies further demonstrate HA’s high adsorption capacity and ion-exchange behavior, whereas biomedical research provides indirect evidence of biological compatibility and controlled interaction with active agents. Nevertheless, direct experimental evidence in food packaging remains limited, including polymer–filler incompatibility, nanoparticle migration risks, and regulatory compliance. This review, therefore, positions hydroxyapatite as a prospective rather than validated packaging additive and outlines critical research priorities, including quantitative migration testing, formulation optimization, and regulatory evaluation, required to assess its practical feasibility in safe and sustainable food packaging applications.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100370"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of microencapsulated Ocimum basilicum essential oil on Cisplatin-Induced hepatic Damage: Modulation of oxidative stress and liver enzymes","authors":"Seyedeh Fatemeh Khalilollahi Ghouchan Atigh ,&nbsp;Viana Khojasteh ,&nbsp;Amirhossein Hajizadeh ,&nbsp;Niknaz Najib ,&nbsp;Farnaz Bemani ,&nbsp;Negin Shafaei ,&nbsp;Ehsan Oskoueian ,&nbsp;Ehsan Karimi","doi":"10.1016/j.crbiot.2026.100377","DOIUrl":"10.1016/j.crbiot.2026.100377","url":null,"abstract":"<div><div>Cisplatin (CP) is a widely utilized chemotherapeutic agent; however, its clinical effectiveness is often limited by dose-dependent hepatotoxicity, which arises from oxidative stress and inflammation. In contrast, bioactive compounds derived from medicinal plants exhibit promising hepatoprotective properties, demonstrating potent antioxidant and anti-inflammatory effects with fewer side effects. This study explored the hepatoprotective potential of microcapsules loaded with <em>Ocimum basilicum</em> essential oil (MLOB) against cisplatin-induced liver injury in mice, specifically examining the modulation of oxidative stress and liver function biomarkers. In this investigation, twenty-four male Balb/c mice were randomly divided into four groups. The control group (T1) received a standard regimen, while the remaining three groups (T2, T3, and T4) were administered CP via intraperitoneal injections to induce hepatotoxicity. Group T2 received normal food, whereas groups T3 and T4 were treated with MLOB at concentrations of 50 mg/kg and 100 mg/kg, respectively. The results indicated that the synthesized microcapsules exhibited suitable particle size, polydispersity index, and zeta potential measurements of 349.3 nm, 0.65, and −31.6 mV, respectively. Administration of cisplatin led to a significant decrease in weight gain, along with elevated liver enzyme levels and oxidative stress markers, coupled with a dysregulation of hepatic antioxidant defense mechanisms such as CAT, SOD, and GPx. Conversely, treatment with MLOB notably mitigated cisplatin-induced hepatotoxicity, as evidenced by improvements in body weight, normalization of serum biochemical parameters, and enhancement of antioxidant enzyme activity. Furthermore, MLOB down-regulated the expression of iNOS and IFN-γ in the liver while up-regulating SOD expression. These findings underscore that microencapsulation significantly enhances the protective efficacy of <em>O. basilicum</em> essential oil, emphasizing its potential as a natural hepatoprotective strategy against liver damage induced by chemotherapy.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100377"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting for reproducing the tumor microenvironment in cancer research models","authors":"Jessy Aoun ,&nbsp;Hugo de Oliveira ,&nbsp;Shane Fennell ,&nbsp;Mona Diab-Assaf ,&nbsp;Florence Caldefie-Chezet ,&nbsp;Laetitia Delort","doi":"10.1016/j.crbiot.2026.100385","DOIUrl":"10.1016/j.crbiot.2026.100385","url":null,"abstract":"<div><div>The tumor microenvironment (TME) is an intricate network of extracellular matrix (ECM) components with different stromal cells, notably immune cells, adipocytes, fibroblasts, endothelial cells that influence cancer progression and therapeutic response. Traditional 2D models fail to replicate the spatial and biomechanical side of the tumor while animal models often lack translational accuracy and pose ethical challenges. 3D bioprinting is a novel technology that recently emerged as an alternative in cancer research, enabling controlled positioning of bioink-encapsulated cells, composed of biomaterials that replicate the TME’s architecture and physiology. This review focuses on depicting some major bioprinting techniques and bioinks used with a focus on their biochemical properties. We further discuss applications of 3D bioprinting in the recreation of the TME in various cancers including breast, ovarian, brain, skin, colorectal, liver, pancreatic and lung cancers. The discussed models emphasize their use for drug screening, study of tumor-stroma interaction and study of the TME in tumor progression. Beyond advancing cancer biology research, these models provide promising tools for drug evaluation and personalized treatment strategies. Despite advances, challenges remain in standardizing bioink formulations, achieving long-term culture stability, and integrating vascular and immune components to fully replicate native tumor complexity. Addressing these issues will be critical to advancing 3D bioprinted tumor models toward clinical and translational applications.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100385"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147706943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of a lignin-degrading Escherichia sp. strain SFD3 and its potential for lignocellulosic biomass valorization","authors":"Amir Safdarianghomsheh ,&nbsp;Mohammad Ali Asadollahi ,&nbsp;Zahra Etemadifar ,&nbsp;Hamid Amiri","doi":"10.1016/j.crbiot.2026.100396","DOIUrl":"10.1016/j.crbiot.2026.100396","url":null,"abstract":"<div><div>In this study, a lignin-degrading bacterial strain, designated SFD3, was isolated from soil and assigned to the genus <em>Escherichia</em>. Molecular identification based on 16S rRNA gene sequencing revealed 98.46% similarity to <em>Escherichia coli</em> and a maximum similarity of 98.66% to <em>Escherichia fergusonii</em>, indicating that precise species-level classification is inconclusive. Therefore, the isolate was conservatively designated as <em>Escherichia</em> sp. SFD3. Phenotypic characterization on eosin methylene blue (EMB) agar showed green metallic sheen colonies, consistent with characteristics commonly associated with <em>E. coli</em>, supporting its affiliation within the genus. The corresponding sequencing information was registered in the NCBI database under accession number PQ368848. To evaluate ligninolytic potential, primers (ECF &amp; ECR) targeting the laccase-like multicopper oxidase (CueO) gene were designed, confirming its presence (accession number PQ493743). This enzyme showed a great capability in oxidizing 3,4-dimethoxy benzyl alcohol (veratryl alcohol), 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and 2,6-dimethoxyphenol (2,6-DMP) with the highest activities of 340 U L⁻¹, 4850 U L⁻¹, and 70 U L⁻¹, respectively. Low-molecular-weight compounds, including different alkanes and phenols, produced by the strain SFD3 during the delignification process were assessed and identified by GC–MS and FT-IR, and the lignin removal rate by this isolate was measured at 44% after 8 days of incubation. Overall, the discovery of a native <em>Escherichia</em> strain with inherent lignin-degrading capability is noteworthy, as members of this genus are not typically associated with ligninolytic activity. This finding suggests the potential of <em>Escherichia</em> sp. SFD3 as a promising candidate in biorefinery platforms for the valorization of lignocellulosic biomass.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100396"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148193489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensified recombinant production of pre-miR-29b-1 through continuous perfusion cultivations","authors":"Francesco Iannacci,&nbsp;Antonio Salza,&nbsp;Fani Sousa,&nbsp;Mattia Sponchioni","doi":"10.1016/j.crbiot.2026.100409","DOIUrl":"10.1016/j.crbiot.2026.100409","url":null,"abstract":"<div><div>Nowadays, RNA therapies represent a cutting-edge therapeutic modality for several human conditions, due to their targeting of the genetic cause of the disease. Within this group, increasing interest is converging onto microRNAs, short non-coding RNA sequences playing a crucial role in cellular processes. In this framework, recent studies assessed the direct correlation between the downregulation of microRNA-29b (miR-29b) and the progression of Alzheimer’s disease, postulating the administration of this oligonucleotide as a treatment for this condition. Despite the great potential, the current manufacturing approaches mainly based on <em>in vitro</em> synthesis are featured by scalability issues, large environmental footprint, and a final product characterized by high immunogenic responses when administrated. These issues may be alleviated by the recombinant production of pre-miR-29b-1. However, this has been reported so far at very small scales and titers, definitely not translatable to a manufacturing scale. In this work, continuous perfusion cultures of the transformed marine bacterium <em>Rhodovulum sulfidophilum</em> were established for the recombinant production of pre-miR-29b-1 with high throughput. Starting from batch liter-scale cultures, the study assessed the role of sodium chloride content in the expansion medium, highlighting a trade-off between microbial growth and product release at increasing salt concentration. From here, we developed the first example of perfusion process for pre-miR-29b-1 biomanufacturing, which allowed to increase the volumetric productivity by 4 times compared to batch operations, in the best-case scenario. These outcomes underline the potential combination of recombinant technology and perfusion cultures to improve process throughput in a sustainable manner.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100409"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148500876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-genome editing: Revolutionizing beneficial Bacteria and Fungi for sustainable agriculture","authors":"Gabriel Moura Mascarin,&nbsp;Maicon Sérgio Nascimento dos Santos,&nbsp;Marcio Vinícius de Carvalho Barros Côrtes","doi":"10.1016/j.crbiot.2026.100404","DOIUrl":"10.1016/j.crbiot.2026.100404","url":null,"abstract":"<div><div>The manipulation of beneficial microorganisms transcends conventional biotechnology to become the biogenic infrastructure of a resilient agriculture. Although the CRISPR/Cas9 system has established itself as a superior genome editing tool, mainly due to its precision, simplicity, and cost-effectiveness, its full translation into agromicrobial ecosystems faces critical biological and technical bottlenecks. This review comprehensively evaluates the implementation of CRISPR systems in fundamental taxa, such as entomopathogenic fungi, <em>Trichoderma</em>, <em>Bacillus</em>, and diazotrophic bacteria, confronting systemic challenges that include the cytotoxicity of Cas9 overexpression, the frequency of off-target mutations, and structural instability in organisms. Additionally, Brazilian bioinnovation has subverted these limitations through the development of marker-free genomic architectures and the optimization of virulence factors in biocontrol agents, establishing a new paradigm of microbial engineering. The evolution of these tools requires not only the improvement of delivery methods, currently inefficient in several species, but also a radical modernization of regulatory frameworks. This paper discusses how the legal classification of these organisms under Brazilian biosafety will shape the future of intellectual property (IP) and the commercial viability of next-generation microbial biopesticides and inoculants. Ultimately, it proposes that overcoming these technical obstacles and changing public perception are imperative for genome editing to catalyze a sustainable revolution in global agricultural productivity.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100404"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148453074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic and technological properties of lactic acid bacteria isolated from palm wine (Bandji) of Côte D'ivoire: An in vitro assessment","authors":"Hanzi Karen Kouamé,&nbsp;Audrey Herbert Yépié,&nbsp;Moussan Désirée Francine Aké,&nbsp;Marina Christelle Assohoun-Djéni,&nbsp;N'Dédé Théodore Djéni,&nbsp;Marcellin Koffi Djè","doi":"10.1016/j.crbiot.2026.100428","DOIUrl":"10.1016/j.crbiot.2026.100428","url":null,"abstract":"<div><div>Technological properties and probiotic aptitude analysis of 10 Lactic Acid Bacteria (LAB) strains, including growth tests, indicated that all strains could survive at 3% NaCl, with optimal growth for strains <em>Fructobacillus durionis</em>_S128 and <em>Lactococcus lactis</em>_S7 at 6.5% NaCl. None grew at 4 °C, but strains <em>Fructobacillus durionis</em>_S24, <em>Fructobacillus durionis</em>_S28, <em>Leuconostoc pseudomesenteroides</em>_S135, and S7 demonstrated abundant growth at 45 °C. pH tolerance varied, with some strains growing at pH 2 and robust growth at pH 6.5 and 8. Enzymatic profiling revealed seven enzymes with varied activity across strains, notably leucine arylamidase and valine arylamidase. Strains <em>Lactococcus lactis</em>_S97, <em>Leuconostoc mesenteroides</em>_S159, S128, and <em>Lacticaseibacillus paracasei</em>_S43 showed favourable enzyme profiles. Antimicrobial tests showed effective inhibition against <em>E. coli, B. cereus</em>, <em>P. aeruginosa</em>, <em>S. aureus</em> and <em>S. typhi</em>, with all LAB strains sensitive to streptomycin and amoxicillin, exhibiting varying susceptibility to other antibiotics. Notably, all strains were resistant to penicillin and several to vancomycin; this resistance is not innocuous and represents a potential safety concern that requires genomic verification (screening for transferable resistance genes) before any application. Cell hydrophobicity indicates that three strains S28 (62.98%), S43 (59.68%), and S97 (58.88%) display hydrophobicity percentages greater than 50%. Concerning bile salt resistance, LAB strains showed high viability rates (50–98%), with the <em>Fructobacillus durionis</em>_S89 strain demonstrating the best resistance (97.67%) and the S97 strain the lowest (51.27%). The investigation for pepsin resistance revealed that all strains can withstand a 0.3% (<em>w</em>/<em>v</em>) pepsin solution at pH 3, maintaining viability rates between 38.40% and 48.5% after 3 h. In the same way, four strains were markedly more resistant than the others at pH 2: S97 (77.45%), S24 (67.52%), S135 (54,94%) and S43 (51.24%). The viability test of LAB strains exposed to a 0.5% pancreatin solution for 4 h at pH 8 showed over 50% resistance, with viability rates ranging from 75% to 95%. The S43 strain exhibited the highest rate at 94.86%, while 80% of the strains tested, including S128 (92.69%), S97 (90.43%), S7 (90.11%), S24 (88.71%), S28 (82.57%), S135 (82.39%) and S89 (82.26%) surpassed 80% viability.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100428"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm of Oenococcus oeni on yeast derivatives: An innovation to trigger malolactic fermentation and modulate the molecular fingerprint of wines","authors":"Tiffany Bellanger ,&nbsp;Estelle Fontaine ,&nbsp;Rémy Romanet ,&nbsp;Yves Gosselin ,&nbsp;Evelyne Fonchy-Penot ,&nbsp;Arnaud Delaherche ,&nbsp;Stéphanie Weidmann","doi":"10.1016/j.crbiot.2026.100375","DOIUrl":"10.1016/j.crbiot.2026.100375","url":null,"abstract":"<div><div>The wine industry is undergoing a major transformation and faces a number of challenges. One of these is climate change, which will significantly increase the alcohol content of wines over the coming decades. This rise in alcohol content will be particularly detrimental to the yeasts and bacteria involved in fermentation, especially those responsible for malolactic fermentation (MLF). To remedy this situation, winemakers are already adopting various strategies, such as using ferments selected for their stress resistance and fermentation efficiency. Among these solutions, the use of ferments under the form of single (<em>O. oeni</em>) or mixte biofilm combining <em>Saccharomyces cerevisiae</em> and <em>Oenococcus oeni</em>, have demonstrated their ability to trigger MLF and influence the organoleptic characteristics of the wine. In<!--> <!-->this context, the aim of this research is to design new, innovative and easy-to-use ferments, by developing an <em>O. oeni</em> biofilm on a biotic support consisting of inactivated yeast in the form of bark. The effectiveness of these formulations was evaluated in microvinification, analyzing their impact on MLF and on the molecular diversity of wines (metabolome and volatilome). The results show an acceleration of MLF, attributed to the presence of yeast derivatives, as well as a complexification of the wine’s chemical properties, favored by the effect of biofilm and yeast derivatives. These new starters thus represent a promising solution for optimizing vinification and enriching the sensory profile of wines.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100375"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence and machine learning across vaccine lifecycle: a systematic review","authors":"Olalekan John Okesanya ,&nbsp;Mulki Mukhtar Hassan ,&nbsp;Uthman Okikiola Adebayo ,&nbsp;Ifrah Ali ,&nbsp;Mohamed Mustaf Ahmed ,&nbsp;Tolutope Adebimpe Oso ,&nbsp;Zhinya Kawa Othman ,&nbsp;Edgar G. Cue ,&nbsp;Aaron Duarte Espinas ,&nbsp;Don Eliseo Lucero-Prisno III","doi":"10.1016/j.crbiot.2026.100379","DOIUrl":"10.1016/j.crbiot.2026.100379","url":null,"abstract":"<div><h3>Introduction</h3><div>Recurrent global health emergencies have intensified interest in the application of artificial intelligence (AI) and machine learning (ML) to all stages of the vaccine life cycle. This systematic review synthesizes the available evidence and maps the current breakthroughs, persistent gaps, and future opportunities for AI- and ML-enabled vaccinology.</div></div><div><h3>Methods</h3><div>Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines, we searched PubMed, Scopus, Embase, ScienceDirect, Cochrane Library, and Google Scholar for peer-reviewed articles published from January 2010 to April 2025. Eligible studies reported any AI/ML application in vaccine discovery, development, manufacturing, supply chain management, or equitable deployment. Two reviewers independently screened the titles, abstracts, and full texts, extracted the data, and assessed the risk of bias using Joanna Briggs Institute tools. The findings were collated using qualitative synthesis.</div></div><div><h3>Results</h3><div>A total of 119 studies examined AI/ML applications across the vaccine lifecycle, including innovation and discovery, development, supply chain optimization, and equitable deployment. AI/ML approaches have improved epitope mapping, candidate screening, safety prediction, demand forecasting, and delivery efficiency, demonstrating their potential to accelerate vaccine development and enhance equitable access.</div></div><div><h3>Conclusion</h3><div>AI/ML tools are transforming the vaccine lifecycle by accelerating epitope prediction, candidate screening, demand forecasting, and equitable allocation; however, their full potential is limited by data silos, algorithmic bias, and uneven validation across populations and settings. Recognizing their impact requires equity-focused strategies, cross-disciplinary collaboration, ethical governance, open data standards, and research on implementation, cost-effectiveness, and explainable AI to ensure equitable and trustworthy vaccine systems.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100379"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}