Current Research in Biotechnology最新文献

{"title":"Exploring the innovation landscape of ginger: Insights from patent documentation analysis","authors":"Maima Matin ,&nbsp;Bhupinder Kapoor ,&nbsp;Monica Gulati ,&nbsp;Michel-Edwar Mickael ,&nbsp;Farhan Bin Matin ,&nbsp;Agnieszka Kamińska ,&nbsp;Joanna Bensz ,&nbsp;Dariusz Sołdacki ,&nbsp;Agnieszka Wierzbicka ,&nbsp;Monika Marcinkowska-Lesiak ,&nbsp;Ahmed Fatimi ,&nbsp;Hong-Yan Liu ,&nbsp;Ren-You Gan ,&nbsp;Olga Adamska ,&nbsp;Artur Stolarczyk ,&nbsp;Jarosław Olav Horbańczuk ,&nbsp;Artur Jóźwik ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100299","DOIUrl":"10.1016/j.crbiot.2025.100299","url":null,"abstract":"<div><div><em>Zingiber officinale</em>, commonly known as ginger, holds a prominent place in both culinary and medicinal practices worldwide. This study is the first to present a comprehensive analysis of ginger-related patents, aiming to elucidate patterns and trends in innovation surrounding this versatile plant. The analysis, conducted using The Lens database, encompasses 7193 patent documents retrieved on April 26, 2025. Annual patent counts illustrate a significant increase in ginger-related patents over the years, with a notable rise observed since 2010. Analysis of the Cooperative Patent Classification system provides a structured framework for organizing these patents, revealing predominant covered categories such as food compositions, plant extracts, and tea substitutes. Additionally, the top-cited patents underscore the diverse applications of ginger, ranging from therapeutic formulations to food and beverage additives. Furthermore, examination of frequently mentioned diseases in ginger patents identifies respiratory ailments, digestive disorders, and skin conditions as key therapeutic areas. These findings show the multifaceted nature of ginger’s pharmacological properties and its potential in addressing various health challenges. Overall, by focusing on ginger as a key food and medicine plant, this study highlights the significance of patent analysis in understanding the innovation landscape of medicinal plants and nutraceuticals, and is specifically opening the ways for future research and development in ginger utilization for global health promotion and sustainable development.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Favipiravir vs. Deferiprone: tautomeric, photophysical, in vitro biological studies, and binding interactions with SARS-Cov-2-MPro/ACE2” [Curr. Res. Biotechnol. 7 (2024) 100176]","authors":"Nikolay T. Tzvetkov ,&nbsp;Martina I. Peeva ,&nbsp;Maya G. Georgieva ,&nbsp;Vera Deneva ,&nbsp;Aneliya A. Balacheva ,&nbsp;Ivan P. Bogdanov ,&nbsp;Maria Ponticelli ,&nbsp;Luigi Milella ,&nbsp;Kiril Kirilov ,&nbsp;Maima Matin ,&nbsp;Hans-Georg Stammler ,&nbsp;Atanas G. Atanasov ,&nbsp;Liudmil Antonov","doi":"10.1016/j.crbiot.2025.100286","DOIUrl":"10.1016/j.crbiot.2025.100286","url":null,"abstract":"","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100286"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species","authors":"Naheem Adekilekun Tijani ,&nbsp;Joseph Hokello ,&nbsp;Emmanuel Eilu ,&nbsp;Saheed Adekunle Akinola ,&nbsp;Abdullateef Opeyemi Afolabi ,&nbsp;Ibrahim Ntulume ,&nbsp;Ismail Abiola Adebayo","doi":"10.1016/j.crbiot.2025.100279","DOIUrl":"10.1016/j.crbiot.2025.100279","url":null,"abstract":"<div><div>Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild <em>Termitomyces</em> species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic <em>Candida</em> species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the <em>Candida</em> isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild <em>Termitomyces</em> mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant <em>Candida</em> pathogens.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation","authors":"Gui-yu Li ,&nbsp;Ji-yong Lin","doi":"10.1016/j.crbiot.2024.100270","DOIUrl":"10.1016/j.crbiot.2024.100270","url":null,"abstract":"<div><h3>Objective</h3><div>Hepatocellular carcinoma <strong>(</strong>HCC) is the most common type of liver cancer and is one of the most common global cancers. <em>Curcuma zedoaria (Christm.) Roscoe</em> is a traditional Chinese herb that has been used for thousands of years in China to treat various types of cancer. Curcumol is one of its primary bioactive sesquiterpenes and has been reported to possess antitumor properties; however, the underlying mechanisms in hepatocellular carcinoma (HCC) are largely unknown. The aim of this study was to reveal the mechanism of curcumol treating HCC based on the network pharmacology and experimental verification.</div></div><div><h3>Materials and Methods</h3><div>Targets of HCC and curcumol were identified. The drugs and disease targets were intersected by <em>Venn Diagram.</em> The KEGG pathway enrichment analysis of curcumol treating HCC was analyzed through the R 3.6.1 software. The effects of curcumol on the inhibition of HCC cell line HepG2 growth and its pro-apoptotic activity were evaluated by cell counting kit-8 and flow cytometry. The expression of microRNA-124 (miRNA-124) mRNA was detected by quantitative real-time PCR. HepG2 cells were transfected with a miRNA mimic and inhibitor. The expression of STAT3 and its phosphorylation were induced by IL-6 and detected by western blotting.</div></div><div><h3>Results</h3><div>MicroRNAs in cancer is a significant enrichment signaling pathway for curcumol treating HCC, according to the KEGG pathway analysis. Curcumol effectively inhibited HepG2 cell growth at 50–150 μg/ml, while it had low toxicity to normal LO2 cells. Using flow cytometry, curcumol strongly promoted apoptosis in HepG2 cells and was more potent than the miRNA-124 mimic, whereas the miRNA-124 inhibitor reduced the pro-apoptotic effect of curcumol. Western blotting revealed that curcumol significantly downregulated the overexpression of STAT3 and its phosphorylation in interleukin-6 induced HepG2 cells, whereas an increased level of STAT3 was observed in the miRNA-124 inhibitor transfected cells after curcumol treatment compared to untransfected cells. The level of miRNA-124 was changed up to 5.87-fold by curcumol treatment.</div></div><div><h3>Conclusions</h3><div>The mechanism underlying the effect of curcumol on inhibition and pro-apoptosis of HepG2 cell growth is possibly related to the miRNA-124/STAT3 pathway.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100270"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity","authors":"Taru Koitto ,&nbsp;Deepika Dahiya ,&nbsp;Martina Huusela ,&nbsp;Merja Penttilä ,&nbsp;Emma Master","doi":"10.1016/j.crbiot.2025.100296","DOIUrl":"10.1016/j.crbiot.2025.100296","url":null,"abstract":"<div><div>Expansins and expansin-like proteins are found in plants and microbes, and can disrupt the cellulosic microfibril network of plant cell walls. While plant expansins play a role in cell wall formation, microbial expansin-like proteins reportedly enhance the activity of lignocellulolytic enzymes. Herein, two novel fungal expansin-like proteins, <em>Ama</em>EXLX1 from <em>Allomyces macrogynus</em> and <em>Apu</em>EXLX1 from <em>Aureobasidium pullulans</em>, were recombinantly produced in <em>Pichia pastoris</em>. While both <em>Ama</em>EXLX1 and <em>Apu</em>EXLX1 retain typical expansin structure, they share low sequence identity (22.5 %) and different predicted pI values (5.8 and 8.8, respectively), which was expected to impact their action on cellulosic substrates. Accordingly, adsorption of the proteins on cellulose nanofibrils (CNF) and the impact of the expansin-like proteins on the hydrolysis of CNF by an endoglucanase (Cel7B) was investigated using quartz crystal microbalance with dissipation (QCM-D). <em>Ama</em>EXLX1 showed higher affinity towards cellulose compared to <em>Apu</em>EXLX1, which was correlated to missing key aromatic residues in the polysaccharide binding surface of <em>Apu</em>EXLX1. The pretreatment of a CNF film with <em>Ama</em>EXLX1 and <em>Apu</em>EXLX1 increased the initial rate of Cel7B activity. This study underscores similarities between the impacts that bacterial and fungal expansin-like proteins can have on the enzymatic deconstruction of cellulose, and sequence properties that could impact expansin performance.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible domestic animal blood products for biomedical and disease treatment applications","authors":"Zhi Cao ,&nbsp;Imran Mahmood Khan ,&nbsp;Xiangrui Niu ,&nbsp;Yang Hu ,&nbsp;Zhuang Duan ,&nbsp;Qiang Fang ,&nbsp;Zhouping Wang ,&nbsp;Junsong Yang","doi":"10.1016/j.crbiot.2025.100308","DOIUrl":"10.1016/j.crbiot.2025.100308","url":null,"abstract":"<div><div>Edible blood products from domestic livestock are high-value derivatives produced during slaughter, with significant potential in biomedicine and disease treatment. Livestock blood is rich in proteins, peptides, iron, vitamins and other bioactive substances. Among them, functional proteins and peptides have been widely studied for their prominent roles in drug delivery, immune regulation, anti-infection and antioxidation. They significantly impact cancer treatment and immune regulation, providing valuable resources for innovation in the food and pharmaceutical industries. The development and utilization of livestock blood products offer innovative solutions for disease treatment and promote the sustainable development of the food and pharmaceutical industries by reducing the environmental burden of blood waste. However, challenges such as the complexity of blood composition, the variability of bioactive compound effectiveness, and the need for advanced purification techniques limit their broader applications. Additionally, regulatory and safety concerns must be addressed for therapeutic suitability. This review highlights the recent advancements in the biomedical applications of livestock blood products in biomedicine, addressing these challenges and emphasizing their potential to reduce environmental waste and promote sustainable development. This review consolidates current knowledge to provide a foundation for future research, fostering innovation in disease intervention and therapy while addressing utilization barriers.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100308"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ranking economic and environmental performance of feedstocks used in bio-based production systems","authors":"Dania Muhieddine Orfali ,&nbsp;Samir Meramo ,&nbsp;Sumesh Sukumara","doi":"10.1016/j.crbiot.2025.100275","DOIUrl":"10.1016/j.crbiot.2025.100275","url":null,"abstract":"<div><div>Biotechnology offers renewable alternatives for producing food, materials, and numerous functional compounds. While rampant human activities are disrupting planets’ geophysical flows, it is urgent to develop sustainable solutions with novel feedstocks and innovative valorization pathways. With the need to reduce greenhouse gas emissions and enhance circularity, new raw materials termed the next-generation feedstocks (<em>NGFs</em>), such as carbon dioxide, methane, methanol, formic acid, and acetic acid, have emerged as potential feedstocks for bio-based processes. So far, no such review exists that compares the performance of conventional, sugar, lignocellulosic, algae-based feedstocks, and <em>NGFs</em>, which biotechnology could upcycle into a wide range of products. In this review, the economic and environmental performances of the feedstocks are analyzed, and quantifications are presented and standardized based on techno-economic analysis and life cycle assessment models. The main parameters for comparison included the geographical location, unit production cost, and environmental impact categories. The results show that the economic and environmental performances are highly variable among the different feedstocks and their processing routes, also depicting evident tradeoffs. Carbon dioxide, sugar cane molasses and glycerol from waste streams are performing better on assessed indicators overall than other potential feedstocks. Nonetheless, this designed data source is the first step for reliable feedstock selection based on sustainability criteria.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100275"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Synthesis and analgesic activity of new analogs of FELL tetrapeptide containing D-Phe in the first position” [Curr. Res. Biotechnol. 8 (2024) 100249]","authors":"Boryana Borisova ,&nbsp;Hristina Nocheva ,&nbsp;Ivan Iliev ,&nbsp;Marie Laronze-Cochard ,&nbsp;Stéphane Gérard ,&nbsp;Stoyko Petrin ,&nbsp;Dancho Danalev","doi":"10.1016/j.crbiot.2025.100283","DOIUrl":"10.1016/j.crbiot.2025.100283","url":null,"abstract":"","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100283"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights from antiaging-related X discussions: A six-year #Longevity hashtag analysis study","authors":"Joanna Bensz ,&nbsp;Dariusz Sołdacki ,&nbsp;Zilin Ma ,&nbsp;Farhan Bin Matin ,&nbsp;Maima Matin ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100280","DOIUrl":"10.1016/j.crbiot.2025.100280","url":null,"abstract":"<div><div>As social media platforms continue to play an increasingly significant role in shaping public discourse and disseminating scientific information, understanding how longevity and aging-related topics are discussed online has become crucial for researchers and healthcare professionals. This study investigates the global discourse on longevity and aging through the analysis of the hashtag #Longevity on the social media platform X (formerly Twitter) over a six-year period from August 1, 2018, to August 1, 2024. A total of 382 032 posts were shared by 109 935 users across 200 countries. The analysis focused on revealing key themes, geographical distribution, sentiment analysis, and the most frequently mentioned supplements and drugs related to longevity. The results show a high level of engagement with the hashtag, primarily driven by users from the United States, followed by the United Kingdom and Canada. Sentiment analysis revealed predominantly positive attitudes towards longevity-related topics, with a slight but statistically significant (p &lt; 0.0001) decline during and after the COVID-19 pandemic. The study identified nicotinamide mononucleotide, rapamycin, and green tea as the most frequently mentioned supplements or drugs in longevity discussions. Notably, there was a significant increase in discussions about niacin derivatives, particularly nicotinamide mononucleotide, during and after the pandemic period. This study highlights the importance of social media as a tool for gauging public interest and sentiment towards scientific topics like longevity, providing valuable insights for researchers, healthcare professionals, and policymakers to enhance science communication and public engagement.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100280"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a thermal stress microfluidic platform to screen stability of therapeutic proteins in pharmaceutical formulations","authors":"O. Bortone ,&nbsp;S. Fiorenza ,&nbsp;M. Baldassarre ,&nbsp;N. Falco ,&nbsp;M. Amidi ,&nbsp;T. Markkula ,&nbsp;P.A. Netti ,&nbsp;E. Torino","doi":"10.1016/j.crbiot.2025.100273","DOIUrl":"10.1016/j.crbiot.2025.100273","url":null,"abstract":"<div><div>Therapeutic proteins have great potentialities for the care of a wide spectrum of diseases, for which other small synthetic drugs result ineffective. Due to challenges related to their immunogenicity, the journey of biologics into clinics still faces obstacles. Among the causes of protein immunogenicity, their natural propensity to aggregation is crucial, indeed, to study their stability, pharmaceutical formulations are generally exposed to diverse environmental physicochemical conditions. Traditional approaches to explore protein behavior are effort-demanding, lengthy and expensive, resulting in a limited knowledge of biomolecule stability. There is an urgent need to develop faster and more cost-effective technologies for biological formulation development. In this work, the conceptualization, design and implementation of a modular and automated microfluidic platform to provide thermal stress to highly concentrated and viscous pharmaceutical formulations is presented. The microfluidic platform validity in terms of reliability and comparability to a forced degradation batch-wise stimulation is demonstrated by thermally stimulating and analyzing through SE-HPLC (Size Exclusion – High Performance Liquid Chromatography) different high concentration (&gt; 100 mg/ml) therapeutic nanobody-based formulations. Remarkably, the ranking of the formulations returned by the microfluidic thermal stress platform follows the same trend obtained through well-established industrial in-batch stimulations. Furthermore, data coming from microfluidic stimulations well correlates to outcomes coming from industrial methodologies for storage and accelerated stability studies.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100273"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}