Current Research in Biotechnology最新文献

{"title":"Workflow for evaluating enzyme immobilization and performance for continuous flow manufacturing","authors":"Emily M. Luteran,&nbsp;Marc R. Aloisi,&nbsp;Wendi L. Akerley,&nbsp;Robert D. Gilbertson","doi":"10.1016/j.crbiot.2025.100327","DOIUrl":"10.1016/j.crbiot.2025.100327","url":null,"abstract":"<div><div>Enzymes have shown promise in various industries due to their functional specificity, catalytic efficiency, and environmental sustainability. These biological catalysts can be a pivotal component of manufacturing pipelines like continuous flow chemistry. For this, there exists a need to robustly immobilize enzymes on solid supports and assess the effects of the solid supports on catalytic performance and stability. Here, we use an industrially relevant model enzyme, <em>C. ensiformis</em> (Jack bean) urease, to demonstrate immobilization and assess performance in the context of continuous flow manufacturing. Various immobilization strategies were screened focusing on immobilization efficiency, protocol simplicity, and urease biocatalyst kinetics. Based on this, CDI-agarose and NHS-agarose resins were identified as the best-performing immobilization strategies for urease. CDI-agarose-urease and NHS-agarose-urease were then scaled up and applied to a large-scale continuous flow reactor to evaluate product yields, operational stability, and long-term stability. These experiments identified differences in stability and performance depending on the immobilization method tested. This highlights the importance of screening immobilization methods and subsequent enzyme performance for each candidate biocatalyst used in manufacturing to promote optimal performance and stability. As such, this work provides a framework for evaluating enzyme biocatalyst immobilization approaches to improve performance and enable transition into industrial processes.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100327"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and biological study of novel FELL analogs containing L- or d-tyr instead of l-phe in the N-terminus","authors":"Boryana Borisova ,&nbsp;Hristina Nocheva ,&nbsp;Hristina Zlatanova-Tenisheva ,&nbsp;Marie Laronze-Cochard ,&nbsp;Stéphane Gérard ,&nbsp;Dancho Danalev","doi":"10.1016/j.crbiot.2025.100332","DOIUrl":"10.1016/j.crbiot.2025.100332","url":null,"abstract":"<div><div>The high prevalence of pain affecting millions of people worldwide made it a major health problem. At the same time often, inflammation is closely associated to the pain. Thus, creation of molecules with a double effect is a good alternative to the currently existing in the medicinal practice non-steroidal medications. Herein, some modifications in the N- and C-terminus of the tetrapeptide FELL with proven anti-inflammatory properties are performed and the newly synthesized compounds are tested for both analgesic and anti-inflammatory potential. The analgesic and anti-inflammatory activity of the newly synthesized molecules was investigated using Paw-pressure and Carrageenan-Induced Paw Edema tests, respectively, on experimental animals. The results showed a certain “discrepancy” between the analgesic and the anti-inflammatory effects of the newly synthesized substances. Newly synthesized <strong>BB9</strong> and <strong>BB15</strong>, L-Tyr containing C-terminal amide and free acid, respectively, exhibit a more significant analgesic activity compared to those of parent molecules <strong>BB1</strong> and <strong>BB11</strong>, containing L-Phe in the N-terminus. Moreover, taking into account the obtained results for the compounds <strong>BB10</strong> and <strong>BB16</strong>, the preferable substitution is L-Tyr, instead of D-Tyr. It could be concluded that the aromatic OH-function is probably important for the connection with the opioid and cannabinoid receptors. However, the designed structural modifications did not lead to improvement in anti-inflammatory effect compared to those of parent molecules. Moreover, in a context of the positive finding is that all modifications done save the hydrolytic stability of the molecules.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100332"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dark interruption with red or blue LEDs mitigates powdery mildew and enhances bioactive compound accumulation in organically grown strawberries","authors":"Samar Khalil ,&nbsp;Beatrix W. Alsanius ,&nbsp;Most Tahera Naznin","doi":"10.1016/j.crbiot.2025.100313","DOIUrl":"10.1016/j.crbiot.2025.100313","url":null,"abstract":"<div><div>Disease attack of powdery mildew (Podosphaera aphanis) is a major concern in organic strawberry production in tunnels or greenhouses production. We examined the impact of dark period interruption with light from blue and red LEDs on the occurrence of powdery mildew in organically grown strawberries, plant performance and on berry quality. Strawberry cultivars (Fragaria × ananassa cvs. Honeye and Faith) were grown in a climate chamber for two months in pots filled with peat based growing media certified for organic production. The plants were drip irrigated and fertilized with liquid organic fertilizers. They were exposed to a control treatment with 18 h light using white (polychromatic) LEDs and 6 h darkness or with treatments involving white LED treatment for 14 h, followed by darkness, interrupted after 2 h by 4 h of monochromatic blue or red LED exposure and additional 4 h of darkness. A three-factorial experiment including strawberry cultivars, light regimes and pathogen inoculation was performed using six replicates (pots) per cultivar and treatment. Light regime influenced the biomass of strawberry plants irrespective of cultivar, and dark period interruption with using red or blue LEDs promoted fresh biomass of the canopy and roots as compared to the control regime. Dark interruption using blue LEDs enhanced the accumulated berry yield, increased the antioxidant activities and reduced the disease incidence as compared to the control regime and the dark period interruption using red LEDs. Blue LED treatment favored the performance of the strawberry cultivars. The obtained results are of interest for organic strawberry production to be implemented in integrated control strategies for powdery mildew with potential to replace the use of pesticides and enhance product quality.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100313"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaporized hydrogen peroxide sterilization of powered air-purifying respirators: Efficacy and safety assessment","authors":"Yeerzati Tuluhongtayi ,&nbsp;Wenjun He ,&nbsp;Kun Cai ,&nbsp;Weifang Han ,&nbsp;Sheng Zhang ,&nbsp;Jun Liu ,&nbsp;Wenwen Lei ,&nbsp;Zongxing Zhang ,&nbsp;Jiancheng Qi ,&nbsp;Guizhen Wu","doi":"10.1016/j.crbiot.2025.100312","DOIUrl":"10.1016/j.crbiot.2025.100312","url":null,"abstract":"<div><div>The COVID-19 pandemic increased demand for personal protective equipment, necessitating exploration of reprocessing methods. This study evaluates the efficacy and safety of vaporized hydrogen peroxide (VHP) sterilization for potential reuse of three powered air purifying respirator (PAPR) brands (3 M, Kebiao, Honeywell). <em>Geobacillus stearothermophilus</em> spores (ATCC 7953) served as biological indicators, while performance parameters (filtration, airflow, battery) and material integrity were monitored throughout 60 cycles, with H₂O₂ residual concentrations measured after 30 cycles. VHP achieved 100 % sterilization efficacy under both unloaded (48/48 biological indicators) and fully-loaded (132/132 biological indicators) conditions, demonstrating complete microbial inactivation across all sampling sites. However, brand-specific sterilization varied across operational conditions. 3 M units achieved complete sterilization in respiratory conduits when powered off but showed reduced filter efficacy. Kebiao units demonstrated complete sterilization when powered off but reduced efficacy during operation at internal filtration sites. Honeywell units with activated carbon showed poor VHP compatibility, while non-activated carbon units performed better when powered off. After 60 cycles, all PAPRs maintained filtration efficiency &gt; 99.97 % and airflow rates &gt; 120 L/min, with functional battery performance. H₂O₂ residual concentrations significantly exceeded safety thresholds (&lt;1 ppm): 3.2 (2.0,4.6) ppm for 3 M, 6.1 (4.3,7.4) ppm for Kebiao, and 6.5 (4.1,8.2) ppm for Honeywell units, with 3 M showing significantly lower residuals (P &lt; 0.05). This study provides critical evidence supporting VHP as a promising approach for PAPR sterilization. However, brand-specific protocols and extended ventilation procedures are required to ensure effective sterilization and reduce H₂O₂ residues to safe level.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100312"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating biotechnology into diagnostic labs: Innovations in PCR and molecular procedures","authors":"Muhammad Faran Tahir ,&nbsp;Sidra Ishtiaq ,&nbsp;Muhammad Anas ,&nbsp;Ayesha Irfan ,&nbsp;Mariusz Jaremko ,&nbsp;Shah Fahad ,&nbsp;Sezai Ercişli ,&nbsp;Hanan Almahasheer ,&nbsp;Nader R. Abdelsalam","doi":"10.1016/j.crbiot.2025.100325","DOIUrl":"10.1016/j.crbiot.2025.100325","url":null,"abstract":"<div><div>The field of biotechnology has significantly contributed to progress in diagnostic laboratories, especially in the early identification of genetic disorders. Timely detection plays a vital role in the treatment and management of such conditions, and numerous molecular approaches have been established to identify disease markers with high accuracy and sensitivity. The polymerase chain reaction (PCR), a cornerstone technique in molecular biology, has seen various advancements, establishing it as an essential method in diagnostic settings for recognizing genetic abnormalities. PCR-based approaches have transformed the diagnostic process by amplifying trace amounts of DNA, allowing for the identification of mutations linked to a wide range of inherited conditions. This review explores the role of biotechnology in diagnostic labs, emphasizing genetic disease detection through molecular procedures. Special attention is given to innovations in PCR, highlighting new PCR-based techniques such as digital PCR, multiplex PCR, and reverse-transcription PCR, which have improved sensitivity and versatility. Additionally, various PCR methodologies, their diagnostic applications, and inherent limitations are discussed. The role of molecular tools in identifying diseases such as cancer, hereditary disorders, and infectious infections is also explored. The review further outlines the evolution of PCR technologies, their integration into molecular diagnostics, and their potential to improve diagnostic accuracy and enable early disease detection. Ongoing advancements in PCR technologies are transforming diagnostic practices by enabling more accurate, faster, and widely accessible molecular testing. These innovations promise enhanced disease management and better clinical outcomes, heralding a new era in healthcare.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100325"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the innovation landscape of ginger: Insights from patent documentation analysis","authors":"Maima Matin ,&nbsp;Bhupinder Kapoor ,&nbsp;Monica Gulati ,&nbsp;Michel-Edwar Mickael ,&nbsp;Farhan Bin Matin ,&nbsp;Agnieszka Kamińska ,&nbsp;Joanna Bensz ,&nbsp;Dariusz Sołdacki ,&nbsp;Agnieszka Wierzbicka ,&nbsp;Monika Marcinkowska-Lesiak ,&nbsp;Ahmed Fatimi ,&nbsp;Hong-Yan Liu ,&nbsp;Ren-You Gan ,&nbsp;Olga Adamska ,&nbsp;Artur Stolarczyk ,&nbsp;Jarosław Olav Horbańczuk ,&nbsp;Artur Jóźwik ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100299","DOIUrl":"10.1016/j.crbiot.2025.100299","url":null,"abstract":"<div><div><em>Zingiber officinale</em>, commonly known as ginger, holds a prominent place in both culinary and medicinal practices worldwide. This study is the first to present a comprehensive analysis of ginger-related patents, aiming to elucidate patterns and trends in innovation surrounding this versatile plant. The analysis, conducted using The Lens database, encompasses 7193 patent documents retrieved on April 26, 2025. Annual patent counts illustrate a significant increase in ginger-related patents over the years, with a notable rise observed since 2010. Analysis of the Cooperative Patent Classification system provides a structured framework for organizing these patents, revealing predominant covered categories such as food compositions, plant extracts, and tea substitutes. Additionally, the top-cited patents underscore the diverse applications of ginger, ranging from therapeutic formulations to food and beverage additives. Furthermore, examination of frequently mentioned diseases in ginger patents identifies respiratory ailments, digestive disorders, and skin conditions as key therapeutic areas. These findings show the multifaceted nature of ginger’s pharmacological properties and its potential in addressing various health challenges. Overall, by focusing on ginger as a key food and medicine plant, this study highlights the significance of patent analysis in understanding the innovation landscape of medicinal plants and nutraceuticals, and is specifically opening the ways for future research and development in ginger utilization for global health promotion and sustainable development.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine-derived secondary metabolites in oncology: A comprehensive review","authors":"Samah S. Abuzahrah ,&nbsp;Serag Eldin I. Elbehairi ,&nbsp;Tahani Bakhsh ,&nbsp;Ahmed Atwa ,&nbsp;Nouf Juaid ,&nbsp;Reham Hassan Mekky","doi":"10.1016/j.crbiot.2025.100300","DOIUrl":"10.1016/j.crbiot.2025.100300","url":null,"abstract":"<div><div>Marine-derived secondary metabolites are emerging as promising anticancer agents due to their structural diversity and potent bioactivity. Within the scope of this all-encompassing study, the most recent advancements in the identification, characterization, and therapeutic applications of marine-derived compounds in oncology are studied. In this review, we discuss various types of bioactive metabolites that are significant, such as macrolactins, polyketides, terpenes, and peptides that are derived from diverse marine organisms <em>viz</em>., bacteria, fungi, actinomycetes, microalgae, macroalgae, mangroves, sponges, coral reefs, mollusks, and echinodermata Unique compounds from marine organisms exhibit diverse mechanisms that selectively target cancer cells. This minimizes harm to healthy tissues and reduces side effects.“These metabolites interfere with key cancer progression pathways such as immune modulation, apoptosis, angiogenesis, metastasis, and cell cycle regulation. Additionally, they enhance conventional treatments like chemotherapy and immune therapy by overcoming drug resistance, particularly multidrug resistance (MDR) and the persistence of cancer stem cells (CSCs), major contributors to therapy failure. CSCs, characterized by self-renewal and therapy resistance, play a central role in tumor recurrence and drug resistance. This review highlights the novelty of marine metabolites, providing a comprehensive inventory of their clinical and commercial applications while emphasizing their role in advancing green medicine through sustainable drug discovery practices. This review shows the promise of marine-derived secondary metabolites in building the future of cancer therapies by incorporating insights from current studies. It also inspires further exploration of the development of these metabolites as clinically practicable treatments.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100300"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the impact of electrode and membrane modification in stacked microbial fuel cells for wastewater treatment","authors":"Aritro Banerjee,&nbsp;Rajnish Kaur Calay,&nbsp;Somil Thakur,&nbsp;Mohamad Y. Mustafa","doi":"10.1016/j.crbiot.2025.100278","DOIUrl":"10.1016/j.crbiot.2025.100278","url":null,"abstract":"<div><div>This study investigates the efficacy of treating wastewater using microbial fuel cell (MFC) technology to the safe limits for discharge in the environment. It has been demonstrated that MFC directly converts organic matter present in wastewater into energy. The present study uses a cell design based on simple plate geometry, carbon felt electrodes and Nafion117 as proton exchange membrane separating the anode and cathode chambers. The anode was then modified with heat and acid treatment and PEM was treated with PVDF to improve the performance of the cell. Synthetic dairy wastewater with initial COD of 2412 mg/l was used to test the operation of stack consisting of four cells which were hydraulically connected in series. The stack operated with continuous flow of wastewater. COD removal of the feed water was tested in successive cells to achieve the permissible limits for safe discharge of the effluent. COD decreased from 2412 mg/l to 126 mg/l after the fourth cell. For the power output each cell was treated individually. The power density of each cell was directly proportional to the COD of the influent. The power density of the first cell that has the highest COD was measured at 77.9 mW/m², which is two times that for the cell with unmodified anode and membrane. For the first cell COD removal was the highest at 57 % and 2.6 times more than the cell with the unmodified anode and membrane. These results suggest that targeted modifications to the anode and membrane can significantly boost the MFC performance both in terms of COD removal and corresponding power output. Secondly, up to 93.66 % COD removal may be achieved by four cells hydraulically connected in series. The paper offers some insights for stacking options for implementing at scale up of the MFC technology for wastewater treatment plants.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100278"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the UCP1-dependent thermogenesis pathway with CRISPR/Cas9: a new approach to obesity management","authors":"Esmail Karami ,&nbsp;Fatemeh Rostamkhani ,&nbsp;Maasoume Abdollahi ,&nbsp;Mohamadreza Ahmadifard","doi":"10.1016/j.crbiot.2025.100295","DOIUrl":"10.1016/j.crbiot.2025.100295","url":null,"abstract":"<div><div>Obesity is a complex, multifactorial disease characterized by excessive body fat accumulation, which negatively impacts health. Its increasing prevalence has led to a global epidemic, emphasizing the urgent need for innovative and effective treatment strategies. This study aims to explore the potential of CRISPR/Cas9-mediated gene editing to enhance UCP1-dependent thermogenesis, offering a novel approach to obesity management. Uncoupling protein 1 (UCP1), primarily located in the inner mitochondrial membrane of brown adipose tissue (BAT), plays a crucial role in thermogenesis and energy expenditure. By converting stored energy into heat, UCP1 activation enhances calorie burning, helping to regulate body temperature and mitigate obesity-related health risks. Recent advancements in genome editing technologies, particularly CRISPR/Cas9, provide a precise method to modify genes involved in UCP1 expression and activity. This approach holds significant promise for sustainable obesity management by enhancing metabolic efficiency and energy expenditure. This study examines the feasibility of using CRISPR/Cas9 to target the UCP1-dependent thermogenesis pathway for obesity treatment. It explores the mechanisms of CRISPR/Cas9, the role of UCP1 in energy regulation, and potential strategies to enhance thermogenic activity. Our findings highlight the promise of CRISPR-based interventions in metabolic regulation. However, further research is necessary to optimize safety, efficacy, and regulatory considerations before translating these findings into clinical applications.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100295"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bromelain as a multifunctional anticancer agent: targeting proliferation, migration, and apoptosis in breast and liver cancer cells","authors":"Nehad A. Shaer,&nbsp;Nouf S. Al-Abbas","doi":"10.1016/j.crbiot.2025.100341","DOIUrl":"10.1016/j.crbiot.2025.100341","url":null,"abstract":"<div><div>This study investigates the anticancer potential of bromelain, a natural proteolytic enzyme derived from pineapple stems, against two of the most prevalent cancers in Saudi Arabia: MCF-7 breast cancer and HepG-2 liver cancer cells. Cell viability was assessed by MTT assay following treatment with increasing concentrations of bromelain for 24 and 48 h, with Taxol serving as a positive control. The anti-migratory effect was evaluated by wound-healing assays, while apoptosis was quantified using Annexin V/7-AAD staining and flow cytometry. Expression of apoptosis-related genes (BAX and BCL2) was analyzed by real-time PCR. Bromelain significantly reduced cancer cell proliferation and migration in a dose- and time-dependent manner, induced both apoptotic and necrotic cell death, and shifted the BAX/BCL2 ratio toward apoptosis. Collectively, these findings demonstrate that bromelain exerts multi-targeted anticancer effects and highlight its potential as a safe, natural adjunct or alternative to conventional chemotherapy in breast and liver cancers.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100341"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}