Current Research in Biotechnology最新文献

{"title":"Deciphering the therapeutic potential of 3,4-Dihydrocoumarin in cervical cancer: A combined network pharmacology and in vitro study","authors":"Bishnu Prasad Parida ,&nbsp;Suraj Thapliyal ,&nbsp;Anjali Mishra ,&nbsp;Boinapalli Gopichand ,&nbsp;Anu Rohit Melge ,&nbsp;Nidheesh Melethadathil ,&nbsp;Megha Radhakrishnan ,&nbsp;Sunita Singh ,&nbsp;Gopeshwar Narayan","doi":"10.1016/j.crbiot.2026.100381","DOIUrl":"10.1016/j.crbiot.2026.100381","url":null,"abstract":"<div><h3>Background</h3><div>Coumarin derivatives represent a versatile scaffold for anticancer drug discovery. This study explored the therapeutic potential of 3,4-dihydrocoumarin (3,4-DHC), a major bioactive non-curcuminoid metabolite from <em>Curcuma caesia</em>, against cervical carcinoma.</div></div><div><h3>Methods</h3><div>Phytocomponents were characterized by LC-HRMS while pharmacokinetics and safety were assessed using SwissADME, Deep-pk, and ProTox-III. Network pharmacology integrating SwissTarget, GeneCards, and DisGeNET identified candidate targets followed by functional enrichment (DAVID) and hub gene prioritization (CytoNCA). Protein-ligand interactions were probed by AutoDock Vina and validated through 500 ns GROMACS simulations. Expression profiles were examined via GEPIA2 and Human Protein Atlas. <em>In vitro</em> validation employed HeLa and SiHa cells using MTT, Annexin V/PI, mitochondrial potential and ROS assays, ATP determination, and western blot analysis.</div></div><div><h3>Results</h3><div>3,4-DHC displayed favorable oral bioavailability, low toxicity, and drug-likeness. Network analysis highlighted CDK2, MMP9, and AKT1 as critical nodes among the hub genes. Docking predicted strong binding (−7.2 to −8.4 kcal/mol), corroborated by stable and energetically favorable MD interactions. Transcriptomic datasets confirmed overexpression and modulated promoter methylation of these targets in cervical tumors. Functionally, 3,4-DHC inhibited proliferation, induced apoptosis, disrupted mitochondrial homeostasis, reduced ATP levels, and suppressed ROS, indicating impaired bioenergetics.</div></div><div><h3>Conclusion</h3><div>3,4-DHC exerts antitumor activity by targeting oncogenic regulators and triggering mitochondrial dysfunction, but the high concentration range needs to be addressed. These findings suggest 3,4-DHC as a potential anticancer molecule.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100381"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147537720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and validation of Na+/H+ antiporter (NHX) gene family in finger millet under salt stress","authors":"Kasinathan Rakkammal ,&nbsp;Pandiyan Muthuramalingam ,&nbsp;Theivanayagam Maharajan ,&nbsp;Collince Omondi Awere ,&nbsp;Radhakrishnan Umamaheswari ,&nbsp;Stanislaus Antony Ceasar ,&nbsp;Hyunsuk Shin ,&nbsp;Manikandan Ramesh","doi":"10.1016/j.crbiot.2026.100365","DOIUrl":"10.1016/j.crbiot.2026.100365","url":null,"abstract":"<div><div>Salt stress poses a serious challenge to agricultural productivity, so it is important to identify stress resilient genes in underutilized cereals like finger millet (<em>Eleusine coracana</em> (L.) Gaertn.). In this study, we identified and characterized five <em>EcNHX</em> genes that encode Na⁺/H⁺ antiporters and examining their physicochemical properties, structural features and regulatory elements. Subcellular localization analysis revealed that <em>EcNHX1</em>-<em>EcNHX4</em> are located in the vacuolar (Vac), while <em>EcNHX5</em> is located in the plasma membrane (PM). Phylogenetic tree classified them into vac and PM NHX groups, with no members present in the endosomal class. Structural analysis confirmed that all the identified NHXs contain NHX protein domains. Furthermore, three-dimensional structural modeling suggested that all EcNHXs share structural features characteristic of Na⁺/H⁺ antiporters. Protein-Protein interaction networks suggest that EcNHXs interact with major ion transporters, which indicating coordinated roles in ion homeostasis. Gene expression analysis of identified <em>NHXs</em> under salt stress exhibits early upregulation of <em>EcNHX1-3</em> and late upregulation of <em>EcNHX4-5</em>. These results suggested that <em>NHX</em> genes were involved in both immediate and long-term stress responses. Overall, this study enhances our knowledge of the structural and functional variations within the <em>NHX</em> gene family in finger millet and highlights potential candidate genes for future functional validation aimed at enhancing salt tolerance through crop improvement programs.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100365"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgA/IgM chromatographic depletion enables efficient 20-nm virus nanofiltration of mini-pool caprylic-acid IgG: A proof-of-concept study","authors":"Liling Delila ,&nbsp;Daniel Strauss ,&nbsp;Thierry Burnouf","doi":"10.1016/j.crbiot.2026.100389","DOIUrl":"10.1016/j.crbiot.2026.100389","url":null,"abstract":"<div><div>Shortages of human plasma-derived immunoglobulin G (IgG) in low- and middle-income countries, driven by a lack of local plasma fractionation capacity, represent a serious obstacle to the treatment of primary and secondary immunodeficiencies, as well as other diseases for which IgG therapy is indicated. Ensuring virus safety is essential, and small-pore nanofiltration is widely used as an established virus-reduction step for IgG manufacturing. We examined whether removing immunoglobulin A (IgA) and immunoglobulin M (IgM) by anion-exchange chromatography improves the performance of 20-nm nanofiltration applied to small-pool caprylic acid-purified IgG. Cryo-poor plasma was treated with 5% caprylic acid at pH 5.5, concentrated by ultrafiltration, and processed on Fractogel TMAE to deplete IgA and IgM. The IgG flow-through was filtered sequentially through Planova 35N and 20N (or S20N) filters. Direct nanofiltration of caprylic acid–treated IgG with residual IgA and IgM led to rapid membrane clogging and low throughput. In contrast, depletion of IgA and IgM increased filtration capacity more than threefold and stabilized flux. Dynamic light scattering indicated a predominant monomeric IgG population and absence of aggregates after chromatography and nanofiltration. This proof-of-concept process integrates caprylic acid treatment and small-pore nanofiltration as orthogonal, literature-supported virus-reduction steps; however, process-specific virus validation was not performed in this study. Overall, selective IgA/IgM depletion enables robust 20-nm class nanofiltration of small-pool caprylic acid-purified IgG and supports development of scalable workflows using domestic plasma.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100389"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable ultrasound-assisted extraction of Miliusa velutina stem bark: Optimization, in vitro-in silico anti-metabolic syndrome evaluation, and life cycle assessment","authors":"Chong Kim Thien Duc ,&nbsp;Tran Chi Linh","doi":"10.1016/j.crbiot.2026.100386","DOIUrl":"10.1016/j.crbiot.2026.100386","url":null,"abstract":"<div><div>Metabolic syndrome (MetS) is associated with oxidative stress, lipid-glucose dysregulation, and chronic inflammation. <em>Miliusa velutina</em> (Annonaceae), a Southeast Asian plant, remains largely unexplored for its biological potential. This study developed a sustainable ultrasound-assisted extraction process to obtain a polyphenol- and flavonoid-rich extract from <em>M. velutina</em> stem bark and evaluated its MetS-related activities <em>in vitro</em> and <em>in silico</em>. The extraction process was optimal using response surface methodology. Optimal conditions (64 °C, 24 min, 68% ethanol, solvent-to-solid ratio of 1:21) yielded total polyphenol and flavonoid contents of 266.69 mg GAE/g and 207.75 mg QE/g. The extract exhibited strong antioxidant (IC₅₀ = 1.34 ± 0.03–73.80 ± 0.94 µg/mL), anti-inflammatory (IC₅₀ = 5.80 ± 0.04–9.55 ± 0.23 µg/mL), anti-cholesterol, enzyme inhibitory, and antibacterial activities. HPLC identified chlorogenic acid, caffeic acid, rutin, hesperidin, and quercetin. Docking analysis showed that quercetin had the highest binding affinity toward MetS-related enzymes. A life cycle assessment confirmed low environmental impact. Overall, this eco-efficient process yields a bioactive extract with promising anti-MetS potential.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100386"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and pharmacological assessment of pyrrole carboxylic acid derivatives: unexpected pro-inflammatory action of a hexanoic analogue in rats","authors":"Hristina Zlatanova-Tenisheva ,&nbsp;Stanislava Vladimirova ,&nbsp;Yordan Todorov","doi":"10.1016/j.crbiot.2026.100372","DOIUrl":"10.1016/j.crbiot.2026.100372","url":null,"abstract":"<div><div>Pyrrole-based carboxylic acid derivatives are a promising scaffold in medicinal chemistry due to their potential anti-inflammatory and analgesic properties. In this study, we synthesized two series of novel pyrrole derivatives (BB and CC) and evaluated their <em>in vivo</em> antinociceptive and anti-inflammatory activity using male Wistar rats. Compounds were administered intraperitoneally at 40 mg/kg, with metamizole and diclofenac serving as reference drugs. Thermal nociception was assessed via the plantar test, and acute inflammation was induced by carrageenan-mediated paw edema. Reference drugs produced the expected analgesic and anti-inflammatory effects, confirming model reliability. In contrast, none of the BB or CC compounds significantly altered paw withdrawal latency or edema, indicating a lack of analgesic and anti-inflammatory efficacy. Notably, <strong>CC1</strong>, a hexanoic acid derivative, caused a significant increase in paw swelling at 3–4 h post-carrageenan, suggesting a pro-inflammatory effect. Structure-activity analysis revealed that variations in side-chain length and <em>para</em>-substitution on the phenyl ring did not enhance pharmacological activity. The absence of efficacy may be due to limited tissue penetration, insufficient target engagement, or metabolic factors, while the pro-inflammatory effect of <strong>CC1</strong> may involve local accumulation, reactive oxygen species generation, and activation of pro-inflammatory signaling pathways. These findings highlight critical limitations of the current pyrrole scaffold and emphasize the need for thorough <em>in vivo</em> assessment during drug development. Overall, while pyrrole carboxylic acids remain a chemically versatile platform, substantial structural optimization is required to achieve effective analgesic or anti-inflammatory activity.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100372"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel 2-aminobenzimidazolyl-1-hydrazones with antileukemic activity: Potent alternatives to imatinib and ibrutinib","authors":"Neda Anastassova ,&nbsp;Elena Stoyanova ,&nbsp;Denitsa Yancheva ,&nbsp;Tanya Dimova ,&nbsp;Miroslav Rangelov ,&nbsp;Svetla Todinova ,&nbsp;Maya Guncheva","doi":"10.1016/j.crbiot.2026.100378","DOIUrl":"10.1016/j.crbiot.2026.100378","url":null,"abstract":"<div><div>Chronic leukemias are serious, life-threatening blood malignancies that require long-term treatment. Current drug development focuses on precise, targeted therapies to overcome the limitations of conventional chemotherapeutics, such as low efficacy and adverse side effects. In this study, we synthesized a series of novel 2-aminobenzimidazolyl-1-hydrazones through a three-step reaction and structurally characterized the compounds. Their cytotoxic activity was evaluated in K562 cells (a model of chronic myeloid leukemia) and HG-3 cells (a model of chronic lymphocytic leukemia). The mechanism of cell death, apoptosis or necrosis, was assessed by flow cytometry after 24 h of treatment with the most active compounds at their respective IC₅₀ values. To evaluate potential hepatotoxicity during early-stage drug development, cytotoxicity was also tested in HepG2 cells, a widely used hepatic model. Among the four compounds tested, 3-(2-amino-1H-benzo[d]imidazol-1-yl)-N′-(2,3-dihydroxybenzylidene)propanehydrazide (<strong>4a</strong>) and 3-(2-amino-1H-benzo[d]imidazol-1-yl)-N′-(2-hydroxy-4-methoxybenzylidene)propanehydrazide (<strong>4b</strong>) showed the most potent cytotoxic effects, matching or exceeding the activity of clinically used drugs, imatinib and ibrutinib, in their respective cell line models.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100378"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking antimicrobial potential of microalgae on food-borne bacteria: A standardized framework and future directions","authors":"Delphine Rapp ,&nbsp;Dieke Schelvis ,&nbsp;Nigel P. French ,&nbsp;Maxence Plouviez","doi":"10.1016/j.crbiot.2025.100362","DOIUrl":"10.1016/j.crbiot.2025.100362","url":null,"abstract":"<div><div>Foodborne infections are a global challenge, costing billions annually through food losses, trade restrictions, and healthcare expenses. Growing concerns over chemical antimicrobials such as antibiotics, sanitizers, and disinfectants, have driven interest in sustainable bio-control strategies for food systems. Microalgae, which produce a plethora of biomolecules including carbohydrates, lipids, proteins, and various secondary metabolites, represent a promising source of antimicrobial compounds. Despite numerous reports demonstrating antimicrobial activity in microalgal extracts, no microalgae-derived antimicrobials have yet reached commercialization.</div><div>This review focuses on some microalgal species already produced at commercial scale, including those with GRAS status (e.g., <em>Chlorella vulgaris</em> and <em>Chlamydomonas reinhardtii</em>). As for other microalgae-based products (e.g., biofuel oil), successful antimicrobial production depends on identifying key species and strains, optimizing growth conditions, and refining harvesting, cell disruption, and extraction protocols. Although research in this area is expanding, further studies are needed to improve our understanding of antimicrobials synthesis and to assess how these factors influence antimicrobial activity. Commonly used antibacterial assays such as disc diffusion and microdilution have limitations that must be considered when evaluating the antimicrobial activity of microalgal extracts. Overall, inconsistencies in testing and reporting have hindered the clear identification of microalgae as sources of effective antimicrobials. This review proposes a framework for future extract preparation and antimicrobial assessment and discusses future prospects to enhance the discovery and yield of microalgal antimicrobials.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100362"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of in silico prediction model for standardized ileal digestibility of amino acids from protein sources","authors":"Takuya Kikuchi ,&nbsp;Masamichi Takeshita ,&nbsp;Yusuke Adachi ,&nbsp;Yoji Yamada","doi":"10.1016/j.crbiot.2026.100368","DOIUrl":"10.1016/j.crbiot.2026.100368","url":null,"abstract":"<div><div>Digestibility is an important factor in the nutritional evaluation of proteins because it determines the bioavailability of the amino acids derived from them. In this study, we developed a prediction model for standardized ileal digestibility (SID) of amino acids using previously reported pig SID datasets. The prediction was performed using the random forest model with the explanatory variables, including nutrients, protein types, cooking/processing, and other ingredients. Shapley additive explanations and feature importance analysis identified the factors of SID prediction and the correlation between digestibility and nutrient data. The predictability for pig SID data was compared between the prediction model and the reported digestibility data of an existing alternative method–<em>in vitro</em> INFOGEST digestion protocol. Finally, the digestible indispensable amino acid score (DIAAS) evaluation using predicted SID showed high accuracy compared with that using observed SID.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100368"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic matter removal from landfill leachate using a biochar-enhanced microbial electrolytic cell-anaerobic digestion system at different HRT","authors":"Luqi Yuan ,&nbsp;Xin Liu ,&nbsp;Xiaoyan Cao ,&nbsp;Jiahui Gao ,&nbsp;Hang Yu ,&nbsp;Yidi Li ,&nbsp;Chongjun Chen","doi":"10.1016/j.crbiot.2026.100371","DOIUrl":"10.1016/j.crbiot.2026.100371","url":null,"abstract":"<div><div>The integration of microbial electrolytic cell with anaerobic digestion (MEC-AD) system is a promising method for improving landfill leachate treatment. Biochar addition further enhances overall process efficiency. However, the impact of varying hydraulic retention times (HRT) on operational efficiency and microbial dynamics is not well understood. This study examines the effects of different HRT on the degradation efficiency and microbial composition of biochar-amended MEC-AD system. Here, optimal performance was observed at 48 h HRT, achieving 70.67% of chemical oxygen demand (COD) removal efficiency, with a notable enrichment of functional microbial strains, which represented a 67.58% increase compared with the removal efficiency at 24 h of HRT. Furthermore, the COD removal efficiency was enhanced by approximately 4% with biochar addition under 24 h of HRT. Essentially, maintaining appreciable HRT coupled with biochar addition is a plausible strategy for enhanced landfill leachate treatment. Mechanistically, this enhanced system performance, specifically efficient organic matter degradation involved the disruption of key structural components within contaminants, including heterocyclic aromatic hydrocarbons and conjugated unsaturated bonds as indicated by GC–MS analysis. Microbial community dynamics revealed that the addition of biochar and the extension of HRT both facilitate <em>Acidobacteria</em> proliferation, while the addition of biochar particularly promoted the enrichment of functional microbes like <em>Pseudomonas</em>. Essentially, both HRT regulation and biochar addition were critical in microbial community structuring. Enrichment of acidogens including <em>Azoarcus</em> and <em>Longilinea</em>, facilitates the subsequent production of acetic acid production, optimizing carbon metabolism. Finally, investigating the scalability of biochar-amended MEC-AD systems under optimized operational parameter (HRT) conditions is urgent to ensure sustainable enhanced landfill leachate treatment and resource recovery.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100371"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailored bacterial co-cultures improve Tisochrysis lutea growth and nutrient profiles under xenic conditions: a new pathway to improve microalgal production","authors":"Tamára F. Santos ,&nbsp;Beatriz Simões ,&nbsp;Veronica Rossetto ,&nbsp;Hugo Pereira ,&nbsp;Inês B. Maia ,&nbsp;Marta Oliveira ,&nbsp;Aschwin Engelen ,&nbsp;João Navalho ,&nbsp;João Varela","doi":"10.1016/j.crbiot.2025.100361","DOIUrl":"10.1016/j.crbiot.2025.100361","url":null,"abstract":"<div><div>The marine haptophyte <em>Tisochrysis lutea</em> is a valuable source of high-value compounds, including polyunsaturated fatty acids like docosahexaenoic acid, and pigments (e.g., fucoxanthin). However, high production costs and variability remain major challenges for its large-scale application in aquaculture, pharmaceuticals, and biotechnology industries. Therefore, strategies to enhance biomass production and quality are actively explored. In natural environments, <em>T. lutea</em> establishes mutualistic interactions with bacteria to obtain essential nutrients such as vitamin B₁₂, yet the role of bacteria in industrial cultures remains poorly understood. In this study, 145 bacterial strains were isolated and taxonomically identified from industrial <em>T. lutea</em> cultures, with members of the class Gammaproteobacteria and Actinomycetia being the most prevalent. Forty isolates were screened individually in co-culture with <em>T. lutea</em> revealing strain-specific effects on growth and biochemical composition. Seven beneficial strains were used to design 21 tailored bacterial blends. Several consortia enhanced biomass production (up to 74 %) and increased key bioactive compounds, particularly methylcobalamin (up to 300 %). These findings demonstrate the potential of tailored bacterial consortia to enhance <em>T. lutea</em> productivity and nutritional quality under production-relevant xenic conditions, enabling strategic microbiome modulation for specific industrial goals.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100361"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}