Current Research in Biotechnology最新文献_第2页

Corrigendum to “Synthesis and analgesic activity of new analogs of FELL tetrapeptide containing D-Phe in the first position” [Curr. Res. Biotechnol. 8 (2024) 100249] “含d -苯丙氨酸的新型FELL四肽类似物的合成和镇痛活性”的更正[Curr.]。生物技术学报，8 (2024)100249 [j]

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100283

Boryana Borisova , Hristina Nocheva , Ivan Iliev , Marie Laronze-Cochard , Stéphane Gérard , Stoyko Petrin , Dancho Danalev

引用次数: 0

Insights from antiaging-related X discussions: A six-year #Longevity hashtag analysis study 来自与抗衰老相关的X讨论的见解：一项为期六年的#长寿标签分析研究

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100280

Joanna Bensz , Dariusz Sołdacki , Zilin Ma , Farhan Bin Matin , Maima Matin , Atanas G. Atanasov

{"title":"Insights from antiaging-related X discussions: A six-year #Longevity hashtag analysis study","authors":"Joanna Bensz , Dariusz Sołdacki , Zilin Ma , Farhan Bin Matin , Maima Matin , Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100280","DOIUrl":"10.1016/j.crbiot.2025.100280","url":null,"abstract":"<div><div>As social media platforms continue to play an increasingly significant role in shaping public discourse and disseminating scientific information, understanding how longevity and aging-related topics are discussed online has become crucial for researchers and healthcare professionals. This study investigates the global discourse on longevity and aging through the analysis of the hashtag #Longevity on the social media platform X (formerly Twitter) over a six-year period from August 1, 2018, to August 1, 2024. A total of 382 032 posts were shared by 109 935 users across 200 countries. The analysis focused on revealing key themes, geographical distribution, sentiment analysis, and the most frequently mentioned supplements and drugs related to longevity. The results show a high level of engagement with the hashtag, primarily driven by users from the United States, followed by the United Kingdom and Canada. Sentiment analysis revealed predominantly positive attitudes towards longevity-related topics, with a slight but statistically significant (p < 0.0001) decline during and after the COVID-19 pandemic. The study identified nicotinamide mononucleotide, rapamycin, and green tea as the most frequently mentioned supplements or drugs in longevity discussions. Notably, there was a significant increase in discussions about niacin derivatives, particularly nicotinamide mononucleotide, during and after the pandemic period. This study highlights the importance of social media as a tool for gauging public interest and sentiment towards scientific topics like longevity, providing valuable insights for researchers, healthcare professionals, and policymakers to enhance science communication and public engagement.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100280"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a thermal stress microfluidic platform to screen stability of therapeutic proteins in pharmaceutical formulations 热应力微流控平台的设计以筛选药物配方中治疗性蛋白的稳定性

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100273

O. Bortone , S. Fiorenza , M. Baldassarre , N. Falco , M. Amidi , T. Markkula , P.A. Netti , E. Torino

{"title":"Design of a thermal stress microfluidic platform to screen stability of therapeutic proteins in pharmaceutical formulations","authors":"O. Bortone , S. Fiorenza , M. Baldassarre , N. Falco , M. Amidi , T. Markkula , P.A. Netti , E. Torino","doi":"10.1016/j.crbiot.2025.100273","DOIUrl":"10.1016/j.crbiot.2025.100273","url":null,"abstract":"<div><div>Therapeutic proteins have great potentialities for the care of a wide spectrum of diseases, for which other small synthetic drugs result ineffective. Due to challenges related to their immunogenicity, the journey of biologics into clinics still faces obstacles. Among the causes of protein immunogenicity, their natural propensity to aggregation is crucial, indeed, to study their stability, pharmaceutical formulations are generally exposed to diverse environmental physicochemical conditions. Traditional approaches to explore protein behavior are effort-demanding, lengthy and expensive, resulting in a limited knowledge of biomolecule stability. There is an urgent need to develop faster and more cost-effective technologies for biological formulation development. In this work, the conceptualization, design and implementation of a modular and automated microfluidic platform to provide thermal stress to highly concentrated and viscous pharmaceutical formulations is presented. The microfluidic platform validity in terms of reliability and comparability to a forced degradation batch-wise stimulation is demonstrated by thermally stimulating and analyzing through SE-HPLC (Size Exclusion – High Performance Liquid Chromatography) different high concentration (> 100 mg/ml) therapeutic nanobody-based formulations. Remarkably, the ranking of the formulations returned by the microfluidic thermal stress platform follows the same trend obtained through well-established industrial in-batch stimulations. Furthermore, data coming from microfluidic stimulations well correlates to outcomes coming from industrial methodologies for storage and accelerated stability studies.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100273"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of the Oligo-Mouse-Microbiota OMM12 in the peristaltic mixed tubular bioreactor PETR PETR蠕动混合管式生物反应器中寡聚小鼠微生物群OMM12的培养

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100294

David Vorländer, Kristin Hoffmann, Katrin Dohnt

{"title":"Cultivation of the Oligo-Mouse-Microbiota OMM12 in the peristaltic mixed tubular bioreactor PETR","authors":"David Vorländer, Kristin Hoffmann, Katrin Dohnt","doi":"10.1016/j.crbiot.2025.100294","DOIUrl":"10.1016/j.crbiot.2025.100294","url":null,"abstract":"<div><div>The intestinal microbiota plays a crucial role in human health and disease, and is therefore of great interest in various research areas. However, studies with humans are limited and difficult to control, leading to a growing demand for sophisticated bioreactor systems that can mimic human intestinal conditions <em>in vitro</em>. The main objective of this study is to compare the <em>in vitro</em> growth of a defined microbiota in the recently published PEristaltic mixed Tubular bioReactor (PETR) with previously published <em>in vivo</em> data for the same microbiota. PETR simulates various colonic conditions, including peristaltic mixing, dialytic water and metabolite removal, and a temporally constant and longitudinally progressive pH gradient in a continuously operated tubular bioreactor. The Oligo-Mouse-Microbiota OMM<sup>12</sup> was chosen as model microbiota and consists of 12 bacteria representing the major phyla of the mouse intestine. During 10d continuous cultivation in PETR, community composition was regularly analyzed using strain-specific qPCR. The results were consistent with the formation of organic acids measured by HPLC. After approximately 6d, the optical density, concentrations of organic acids, and the microbiota composition remained relatively stable. Despite the different intestinal conditions of humans and mice, several similarities between reactor cultivation and gnotobiotic mouse model confirm PETR as a suitable system for microbiota research.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100294"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogenic silver nanoparticles produced by Trichoderma reesei inhibit SARS-CoV-2 infection, reduce lung viral load and ameliorate acute pulmonary inflammation 里氏木霉产生的生物源银纳米颗粒抑制SARS-CoV-2感染，降低肺部病毒载量，改善急性肺部炎症

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100277

Marcus V.M.V. Amaral , Cláudia B. Carraro , Amanda C.C. Antoniêto , Mariana N. Costa , Thais F.C. Fraga-Silva , Ualter G. Cipriano , Rodrigo P.F. Abuná , Tamara S. Rodrigues , Ronaldo B. Martins , Andreia M. Luzenti , Glaucia R. Caruso , Priscyla D. Marcato , Vania L.D. Bonato , Dario S. Zamboni , Bergman M. Ribeiro , Sônia N. Báo , Joao S. da Silva , Flávio P. Veras , Roberto N. Silva

{"title":"Biogenic silver nanoparticles produced by Trichoderma reesei inhibit SARS-CoV-2 infection, reduce lung viral load and ameliorate acute pulmonary inflammation","authors":"Marcus V.M.V. Amaral , Cláudia B. Carraro , Amanda C.C. Antoniêto , Mariana N. Costa , Thais F.C. Fraga-Silva , Ualter G. Cipriano , Rodrigo P.F. Abuná , Tamara S. Rodrigues , Ronaldo B. Martins , Andreia M. Luzenti , Glaucia R. Caruso , Priscyla D. Marcato , Vania L.D. Bonato , Dario S. Zamboni , Bergman M. Ribeiro , Sônia N. Báo , Joao S. da Silva , Flávio P. Veras , Roberto N. Silva","doi":"10.1016/j.crbiot.2025.100277","DOIUrl":"10.1016/j.crbiot.2025.100277","url":null,"abstract":"<div><div>The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), posed a significant global health challenge and still demands efforts to develop new therapies. In this study, we investigated the potential of biogenic silver nanoparticles (AgNPs) synthesized by the fungus <em>Trichoderma reesei</em> to combat SARS-CoV-2 infection. In silico studies showed that AgNPs, ranging from 7 nm to 50 nm, have high affinity for spike protein from different variant of SARS-CoV-2. Our findings show that AgNPs effectively do not affect cell viability in Calu-3 cells, inhibit viral infection in Vero-E6 cells and progression of infection <em>in vitro</em>. Additionally, AgNPs impair caspase-1 activation, lactate dehydrogenase release and IL-1β production by human monocytes. Moreover, our study reveals that AgNPs treatment significantly alleviated acute lung injury induced by SARS-CoV-2 infection in Syrian hamsters. This suggests that AgNPs treatment effectively impairs viral replication or propagation within lung tissue, highlighting its potential as an antiviral agent against SARS-CoV-2. Further investigations are warranted to elucidate the underlying mechanisms of action of AgNPs and to assess their safety and efficacy in clinical settings. Nonetheless, our findings offer promising insights into the development of novel therapeutic strategies for combating COVID-19 and reducing its associated morbidity and mortality.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100277"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of substrate type and its constituents on overall performance of microbial fuel cells 基质类型及其成分对微生物燃料电池整体性能的重要性

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100272

Somil Thakur, Rajnish Kaur Calay, Mohamad Y. Mustafa, Fasil E. Eregno, Ravindra R. Patil

{"title":"Importance of substrate type and its constituents on overall performance of microbial fuel cells","authors":"Somil Thakur, Rajnish Kaur Calay, Mohamad Y. Mustafa, Fasil E. Eregno, Ravindra R. Patil","doi":"10.1016/j.crbiot.2025.100272","DOIUrl":"10.1016/j.crbiot.2025.100272","url":null,"abstract":"<div><div>Microbial Fuel Cells (MFC) have emerged as a potential wastewater treatment technology that utilizes metabolic processes of microorganisms present in the wastewater to disintegrate organic substrates and harness direct electricity. This paper reviews the potential of different wastewater types as a suitable substrate for microbial activities in MFCs. Substrate composition (carbon source, nutrient content and inhibitory compounds) directly affects the microbial growth, wastewater treatment potential, electron transfer rate and power harvested. Readily biodegradable substrates such as acetate and glucose promote microbial metabolism and electron transport, thus resulting in enhanced power generation. Substrates such as municipal or agricultural wastewater that constitute both simple and complex organic matter require longer breakdown durations but can provide MFCs with long term operational stability. On the other hand, substrates such as leachate from landfills, mining wastewater etc. are generally unsuitable for regular operations. The paper discusses the challenges such as suitability of various wastewaters, unpredictability of composition, presence of inhibitory chemicals like heavy metals or toxic organics that can subdue the microbial activity and reduce efficiency of a MFC reactor. It aims to identify the relationship between the substrate characteristics and MFC performance in order to select the substrate for achieving optimal output from MFC technology. The suitable substrates that exhibit optimal performance in terms of current and power output, coulombic efficiency, and reduction in chemical oxygen demand are identified.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100272"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptides and peptidomimetics in the development of hydrogels towards the treatment of diabetic wounds 水凝胶在糖尿病伤口治疗中的应用

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100292

Ana Gomes, Paula Gomes

{"title":"Peptides and peptidomimetics in the development of hydrogels towards the treatment of diabetic wounds","authors":"Ana Gomes, Paula Gomes","doi":"10.1016/j.crbiot.2025.100292","DOIUrl":"10.1016/j.crbiot.2025.100292","url":null,"abstract":"<div><div>Diabetic foot ulcers are a major complication of diabetes and a particularly complex type of chronic wounds, as most diabetes-related physiological dysregulations impair healing and increase the risk of infection. Despite many health literacy initiatives have been deployed to promote both the prevention and the proper management of diabetic foot ulcers, these remain a serious healthcare problem whose incidence is rising. This demands not only for a continuous updating of guidelines for adequate interventions in healthcare facilities, but also for new topical treatments able to improve the health and well-being of the patients. In this later case, current options include topical oxygen therapy, negative pressure wound therapy, wound dressings of different types, and topical gels. Due to the tremendous potential of hydrogel-based dressings for diabetic wound treatment, several hydrogels from either natural or synthetic origin have been explored. The hydrogel polymeric matrices can be loaded with many diverse molecules (cargoes) eventually combining distinct therapeutic actions. In this context, peptides are gaining relevance, which is not limited to those used as bioactive cargoes, since self-assembling peptides able to form hydrogels are also under the spotlight. This review focuses on the studies reported over the last decade where peptide-loaded or peptide-derived hydrogels have been tested <em>in vivo</em> for topical treatment of diabetic wounds and further explores the potential of peptidomimetics to expand the portfolio of hydrogel-based treatments for diabetic foot ulcers.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100292"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of targeting GPR77+CD10+ lipid nanoparticles and validation of targeting capability in vitro and in vivo 靶向GPR77+CD10+脂质纳米颗粒的构建及体外和体内靶向能力的验证

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100291

Junyue Fang , Qiongchao Jiang , Xinyu Yang , Weifan Li , Li Lin , Meng Zhang , Phei Er Saw , Xiaoyun Xiao

{"title":"Construction of targeting GPR77+CD10+ lipid nanoparticles and validation of targeting capability in vitro and in vivo","authors":"Junyue Fang , Qiongchao Jiang , Xinyu Yang , Weifan Li , Li Lin , Meng Zhang , Phei Er Saw , Xiaoyun Xiao","doi":"10.1016/j.crbiot.2025.100291","DOIUrl":"10.1016/j.crbiot.2025.100291","url":null,"abstract":"<div><div>Dual-receptor targeting strategies hold promise for improving nanocarrier specificity in complex tumor microenvironments. Herein, we engineered lipid nanoparticles (LNPs) functionalized with GPR77 and CD10 antibodies to exploit receptor co-expression as a mechanism for enhanced targeting. To rigorously validate this approach, we developed a GPR77<sup>+</sup>CD10<sup>+</sup> overexpressing CHO cell model, which served as a controlled system to dissect ligand-receptor interactions. The dual-targeting LNPs (DOPE(GPR77/CD10)) exhibited significantly higher cellular uptake in receptor-positive CHO cells compared to single-targeted or non-targeted formulations, demonstrating synergistic binding efficacy. These LNPs also showed excellent drug encapsulation and prolonged circulation. In a CHO xenograft model, dual-targeting LNPs achieved higher tumor accumulation than non-targeted controls, with minimal off-target organ retention. Biosafety assessments confirmed negligible hemolysis and no hepatorenal toxicity. While this study focused on mechanistic validation in a simplified model, our findings establish a generalizable platform for dual-receptor targeting, with potential applications in stromal or tumor cell-specific drug delivery.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100291"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review on Indian Acacia species 文章题目印度金合欢种的系统综述

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100274

Bharat Singh , Jagdish Prasad , Ram Avtar Sharma

{"title":"A systematic review on Indian Acacia species","authors":"Bharat Singh , Jagdish Prasad , Ram Avtar Sharma","doi":"10.1016/j.crbiot.2025.100274","DOIUrl":"10.1016/j.crbiot.2025.100274","url":null,"abstract":"<div><div>Acacia plants are evergreen, perennial, short, flat-crowned to long and straight-bole trees. A total of 34 species of Acacia genus are distributed in different states of India. Acacia plants are used in the treatment of chronic dysentery, diarrhoea, throat troubles, conjunctivitis and stomatitis. The critical data of ethnopharmacological properties, phytochemical and pharmacological attributes of 27 species were obtained through extensive review of relevant reference books, M. Sc./M. Tech dissertations, PhD theses, Web of Science, PubMed, Google Scholar, ScienceDirect, Wiley, Taylors & Francis, Bentham, Thieme, Springer, and eFloras. The flavone glycosides, flavonoids, lignans, anthraquinones, monoterpenes, and alkaloids have been identified from 20 species of Indian Acacia species. Indian Acacia species possess antimicrobial, antidiabetic, antiarthritic, antistress, anti-inflammatory, antioxidant, anticancer, and wound healing properties. However, various reports are available in literature on phytochemistry and biological properties of 27 Indian Acacia species but, still 7 species have not been evaluated for their pharmacological studies. The review discusses updated knowledge of ethnopharmacological applications, phytochemical and pharmacological attributes, and pharmacokinetic profiles of Indian Acacia species.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100274"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes 饲粮中添加姜黄素可提高水牛血清碳水化合物降解酶的活性

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100276

Maima Matin , Natalia Ksepka , Kamil Wysocki , Michel-Edwar Mickael , Alpana Pande , Jarosław Olav Horbańczuk , Artur Jóźwik , Olga Adamska , Artur Stolarczyk , Laszlo Barna Iantovics , Yordanka Ilieva , Pencho Penchev , Atanas G. Atanasov

{"title":"Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes","authors":"Maima Matin , Natalia Ksepka , Kamil Wysocki , Michel-Edwar Mickael , Alpana Pande , Jarosław Olav Horbańczuk , Artur Jóźwik , Olga Adamska , Artur Stolarczyk , Laszlo Barna Iantovics , Yordanka Ilieva , Pencho Penchev , Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100276","DOIUrl":"10.1016/j.crbiot.2025.100276","url":null,"abstract":"<div><div>Curcumin, the major bioactive compound found in turmeric (<em>Curcuma longa</em>) is a subject of intensive research, due to its multiple bioactivities. Previous studies have investigated metabolic effects of curcumin in humans or established animal research models such as mice, but studies with farm animals have been scarce. In this work, we aimed to study the effects of curcumin supplementation in Bulgarian Murrah buffaloes (<em>Bubalus bubalis</em>), on the serum activities of seven important carbohydrate-metabolizing enzymes, β-glucuronidase, α-galactosidase, α-glucosidase, β-glucosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase. Curcumin was supplemented at a dose of 50 g per animal, per day, and serum activities of the studied enzymes were determined at the start (day 0), middle (day 14), and end (day 28) of the supplementation period. A tendency for increased activity of all studied enzymes was observed upon supplementation with curcumin, with the activity levels of five of the enzymes displaying statistically significant upregulation at day 28 (β-glucuronidase, α-galactosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase). These upregulated activities are in general indicative of increased carbohydrates turnover and detoxification processes, and might provide clues for a better understanding of the mechanisms of action of curcumin <em>in vivo</em>, as well as for novel approaches to rationally impact animal or human health and wellbeing through targeted supplementation.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0