Current Research in Biotechnology最新文献

{"title":"Marine-derived secondary metabolites in oncology: A comprehensive review","authors":"Samah S. Abuzahrah ,&nbsp;Serag Eldin I. Elbehairi ,&nbsp;Tahani Bakhsh ,&nbsp;Ahmed Atwa ,&nbsp;Nouf Juaid ,&nbsp;Reham Hassan Mekky","doi":"10.1016/j.crbiot.2025.100300","DOIUrl":"10.1016/j.crbiot.2025.100300","url":null,"abstract":"<div><div>Marine-derived secondary metabolites are emerging as promising anticancer agents due to their structural diversity and potent bioactivity. Within the scope of this all-encompassing study, the most recent advancements in the identification, characterization, and therapeutic applications of marine-derived compounds in oncology are studied. In this review, we discuss various types of bioactive metabolites that are significant, such as macrolactins, polyketides, terpenes, and peptides that are derived from diverse marine organisms <em>viz</em>., bacteria, fungi, actinomycetes, microalgae, macroalgae, mangroves, sponges, coral reefs, mollusks, and echinodermata Unique compounds from marine organisms exhibit diverse mechanisms that selectively target cancer cells. This minimizes harm to healthy tissues and reduces side effects.“These metabolites interfere with key cancer progression pathways such as immune modulation, apoptosis, angiogenesis, metastasis, and cell cycle regulation. Additionally, they enhance conventional treatments like chemotherapy and immune therapy by overcoming drug resistance, particularly multidrug resistance (MDR) and the persistence of cancer stem cells (CSCs), major contributors to therapy failure. CSCs, characterized by self-renewal and therapy resistance, play a central role in tumor recurrence and drug resistance. This review highlights the novelty of marine metabolites, providing a comprehensive inventory of their clinical and commercial applications while emphasizing their role in advancing green medicine through sustainable drug discovery practices. This review shows the promise of marine-derived secondary metabolites in building the future of cancer therapies by incorporating insights from current studies. It also inspires further exploration of the development of these metabolites as clinically practicable treatments.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100300"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the innovation landscape of ginger: Insights from patent documentation analysis","authors":"Maima Matin ,&nbsp;Bhupinder Kapoor ,&nbsp;Monica Gulati ,&nbsp;Michel-Edwar Mickael ,&nbsp;Farhan Bin Matin ,&nbsp;Agnieszka Kamińska ,&nbsp;Joanna Bensz ,&nbsp;Dariusz Sołdacki ,&nbsp;Agnieszka Wierzbicka ,&nbsp;Monika Marcinkowska-Lesiak ,&nbsp;Ahmed Fatimi ,&nbsp;Hong-Yan Liu ,&nbsp;Ren-You Gan ,&nbsp;Olga Adamska ,&nbsp;Artur Stolarczyk ,&nbsp;Jarosław Olav Horbańczuk ,&nbsp;Artur Jóźwik ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100299","DOIUrl":"10.1016/j.crbiot.2025.100299","url":null,"abstract":"<div><div><em>Zingiber officinale</em>, commonly known as ginger, holds a prominent place in both culinary and medicinal practices worldwide. This study is the first to present a comprehensive analysis of ginger-related patents, aiming to elucidate patterns and trends in innovation surrounding this versatile plant. The analysis, conducted using The Lens database, encompasses 7193 patent documents retrieved on April 26, 2025. Annual patent counts illustrate a significant increase in ginger-related patents over the years, with a notable rise observed since 2010. Analysis of the Cooperative Patent Classification system provides a structured framework for organizing these patents, revealing predominant covered categories such as food compositions, plant extracts, and tea substitutes. Additionally, the top-cited patents underscore the diverse applications of ginger, ranging from therapeutic formulations to food and beverage additives. Furthermore, examination of frequently mentioned diseases in ginger patents identifies respiratory ailments, digestive disorders, and skin conditions as key therapeutic areas. These findings show the multifaceted nature of ginger’s pharmacological properties and its potential in addressing various health challenges. Overall, by focusing on ginger as a key food and medicine plant, this study highlights the significance of patent analysis in understanding the innovation landscape of medicinal plants and nutraceuticals, and is specifically opening the ways for future research and development in ginger utilization for global health promotion and sustainable development.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaporized hydrogen peroxide sterilization of powered air-purifying respirators: Efficacy and safety assessment","authors":"Yeerzati Tuluhongtayi ,&nbsp;Wenjun He ,&nbsp;Kun Cai ,&nbsp;Weifang Han ,&nbsp;Sheng Zhang ,&nbsp;Jun Liu ,&nbsp;Wenwen Lei ,&nbsp;Zongxing Zhang ,&nbsp;Jiancheng Qi ,&nbsp;Guizhen Wu","doi":"10.1016/j.crbiot.2025.100312","DOIUrl":"10.1016/j.crbiot.2025.100312","url":null,"abstract":"<div><div>The COVID-19 pandemic increased demand for personal protective equipment, necessitating exploration of reprocessing methods. This study evaluates the efficacy and safety of vaporized hydrogen peroxide (VHP) sterilization for potential reuse of three powered air purifying respirator (PAPR) brands (3 M, Kebiao, Honeywell). <em>Geobacillus stearothermophilus</em> spores (ATCC 7953) served as biological indicators, while performance parameters (filtration, airflow, battery) and material integrity were monitored throughout 60 cycles, with H₂O₂ residual concentrations measured after 30 cycles. VHP achieved 100 % sterilization efficacy under both unloaded (48/48 biological indicators) and fully-loaded (132/132 biological indicators) conditions, demonstrating complete microbial inactivation across all sampling sites. However, brand-specific sterilization varied across operational conditions. 3 M units achieved complete sterilization in respiratory conduits when powered off but showed reduced filter efficacy. Kebiao units demonstrated complete sterilization when powered off but reduced efficacy during operation at internal filtration sites. Honeywell units with activated carbon showed poor VHP compatibility, while non-activated carbon units performed better when powered off. After 60 cycles, all PAPRs maintained filtration efficiency &gt; 99.97 % and airflow rates &gt; 120 L/min, with functional battery performance. H₂O₂ residual concentrations significantly exceeded safety thresholds (&lt;1 ppm): 3.2 (2.0,4.6) ppm for 3 M, 6.1 (4.3,7.4) ppm for Kebiao, and 6.5 (4.1,8.2) ppm for Honeywell units, with 3 M showing significantly lower residuals (P &lt; 0.05). This study provides critical evidence supporting VHP as a promising approach for PAPR sterilization. However, brand-specific protocols and extended ventilation procedures are required to ensure effective sterilization and reduce H₂O₂ residues to safe level.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100312"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dark interruption with red or blue LEDs mitigates powdery mildew and enhances bioactive compound accumulation in organically grown strawberries","authors":"Samar Khalil ,&nbsp;Beatrix W. Alsanius ,&nbsp;Most Tahera Naznin","doi":"10.1016/j.crbiot.2025.100313","DOIUrl":"10.1016/j.crbiot.2025.100313","url":null,"abstract":"<div><div>Disease attack of powdery mildew (Podosphaera aphanis) is a major concern in organic strawberry production in tunnels or greenhouses production. We examined the impact of dark period interruption with light from blue and red LEDs on the occurrence of powdery mildew in organically grown strawberries, plant performance and on berry quality. Strawberry cultivars (Fragaria × ananassa cvs. Honeye and Faith) were grown in a climate chamber for two months in pots filled with peat based growing media certified for organic production. The plants were drip irrigated and fertilized with liquid organic fertilizers. They were exposed to a control treatment with 18 h light using white (polychromatic) LEDs and 6 h darkness or with treatments involving white LED treatment for 14 h, followed by darkness, interrupted after 2 h by 4 h of monochromatic blue or red LED exposure and additional 4 h of darkness. A three-factorial experiment including strawberry cultivars, light regimes and pathogen inoculation was performed using six replicates (pots) per cultivar and treatment. Light regime influenced the biomass of strawberry plants irrespective of cultivar, and dark period interruption with using red or blue LEDs promoted fresh biomass of the canopy and roots as compared to the control regime. Dark interruption using blue LEDs enhanced the accumulated berry yield, increased the antioxidant activities and reduced the disease incidence as compared to the control regime and the dark period interruption using red LEDs. Blue LED treatment favored the performance of the strawberry cultivars. The obtained results are of interest for organic strawberry production to be implemented in integrated control strategies for powdery mildew with potential to replace the use of pesticides and enhance product quality.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100313"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Favipiravir vs. Deferiprone: tautomeric, photophysical, in vitro biological studies, and binding interactions with SARS-Cov-2-MPro/ACE2” [Curr. Res. Biotechnol. 7 (2024) 100176]","authors":"Nikolay T. Tzvetkov ,&nbsp;Martina I. Peeva ,&nbsp;Maya G. Georgieva ,&nbsp;Vera Deneva ,&nbsp;Aneliya A. Balacheva ,&nbsp;Ivan P. Bogdanov ,&nbsp;Maria Ponticelli ,&nbsp;Luigi Milella ,&nbsp;Kiril Kirilov ,&nbsp;Maima Matin ,&nbsp;Hans-Georg Stammler ,&nbsp;Atanas G. Atanasov ,&nbsp;Liudmil Antonov","doi":"10.1016/j.crbiot.2025.100286","DOIUrl":"10.1016/j.crbiot.2025.100286","url":null,"abstract":"","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100286"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species","authors":"Naheem Adekilekun Tijani ,&nbsp;Joseph Hokello ,&nbsp;Emmanuel Eilu ,&nbsp;Saheed Adekunle Akinola ,&nbsp;Abdullateef Opeyemi Afolabi ,&nbsp;Ibrahim Ntulume ,&nbsp;Ismail Abiola Adebayo","doi":"10.1016/j.crbiot.2025.100279","DOIUrl":"10.1016/j.crbiot.2025.100279","url":null,"abstract":"<div><div>Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild <em>Termitomyces</em> species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic <em>Candida</em> species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the <em>Candida</em> isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild <em>Termitomyces</em> mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant <em>Candida</em> pathogens.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation","authors":"Gui-yu Li ,&nbsp;Ji-yong Lin","doi":"10.1016/j.crbiot.2024.100270","DOIUrl":"10.1016/j.crbiot.2024.100270","url":null,"abstract":"<div><h3>Objective</h3><div>Hepatocellular carcinoma <strong>(</strong>HCC) is the most common type of liver cancer and is one of the most common global cancers. <em>Curcuma zedoaria (Christm.) Roscoe</em> is a traditional Chinese herb that has been used for thousands of years in China to treat various types of cancer. Curcumol is one of its primary bioactive sesquiterpenes and has been reported to possess antitumor properties; however, the underlying mechanisms in hepatocellular carcinoma (HCC) are largely unknown. The aim of this study was to reveal the mechanism of curcumol treating HCC based on the network pharmacology and experimental verification.</div></div><div><h3>Materials and Methods</h3><div>Targets of HCC and curcumol were identified. The drugs and disease targets were intersected by <em>Venn Diagram.</em> The KEGG pathway enrichment analysis of curcumol treating HCC was analyzed through the R 3.6.1 software. The effects of curcumol on the inhibition of HCC cell line HepG2 growth and its pro-apoptotic activity were evaluated by cell counting kit-8 and flow cytometry. The expression of microRNA-124 (miRNA-124) mRNA was detected by quantitative real-time PCR. HepG2 cells were transfected with a miRNA mimic and inhibitor. The expression of STAT3 and its phosphorylation were induced by IL-6 and detected by western blotting.</div></div><div><h3>Results</h3><div>MicroRNAs in cancer is a significant enrichment signaling pathway for curcumol treating HCC, according to the KEGG pathway analysis. Curcumol effectively inhibited HepG2 cell growth at 50–150 μg/ml, while it had low toxicity to normal LO2 cells. Using flow cytometry, curcumol strongly promoted apoptosis in HepG2 cells and was more potent than the miRNA-124 mimic, whereas the miRNA-124 inhibitor reduced the pro-apoptotic effect of curcumol. Western blotting revealed that curcumol significantly downregulated the overexpression of STAT3 and its phosphorylation in interleukin-6 induced HepG2 cells, whereas an increased level of STAT3 was observed in the miRNA-124 inhibitor transfected cells after curcumol treatment compared to untransfected cells. The level of miRNA-124 was changed up to 5.87-fold by curcumol treatment.</div></div><div><h3>Conclusions</h3><div>The mechanism underlying the effect of curcumol on inhibition and pro-apoptosis of HepG2 cell growth is possibly related to the miRNA-124/STAT3 pathway.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100270"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity","authors":"Taru Koitto ,&nbsp;Deepika Dahiya ,&nbsp;Martina Huusela ,&nbsp;Merja Penttilä ,&nbsp;Emma Master","doi":"10.1016/j.crbiot.2025.100296","DOIUrl":"10.1016/j.crbiot.2025.100296","url":null,"abstract":"<div><div>Expansins and expansin-like proteins are found in plants and microbes, and can disrupt the cellulosic microfibril network of plant cell walls. While plant expansins play a role in cell wall formation, microbial expansin-like proteins reportedly enhance the activity of lignocellulolytic enzymes. Herein, two novel fungal expansin-like proteins, <em>Ama</em>EXLX1 from <em>Allomyces macrogynus</em> and <em>Apu</em>EXLX1 from <em>Aureobasidium pullulans</em>, were recombinantly produced in <em>Pichia pastoris</em>. While both <em>Ama</em>EXLX1 and <em>Apu</em>EXLX1 retain typical expansin structure, they share low sequence identity (22.5 %) and different predicted pI values (5.8 and 8.8, respectively), which was expected to impact their action on cellulosic substrates. Accordingly, adsorption of the proteins on cellulose nanofibrils (CNF) and the impact of the expansin-like proteins on the hydrolysis of CNF by an endoglucanase (Cel7B) was investigated using quartz crystal microbalance with dissipation (QCM-D). <em>Ama</em>EXLX1 showed higher affinity towards cellulose compared to <em>Apu</em>EXLX1, which was correlated to missing key aromatic residues in the polysaccharide binding surface of <em>Apu</em>EXLX1. The pretreatment of a CNF film with <em>Ama</em>EXLX1 and <em>Apu</em>EXLX1 increased the initial rate of Cel7B activity. This study underscores similarities between the impacts that bacterial and fungal expansin-like proteins can have on the enzymatic deconstruction of cellulose, and sequence properties that could impact expansin performance.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible domestic animal blood products for biomedical and disease treatment applications","authors":"Zhi Cao ,&nbsp;Imran Mahmood Khan ,&nbsp;Xiangrui Niu ,&nbsp;Yang Hu ,&nbsp;Zhuang Duan ,&nbsp;Qiang Fang ,&nbsp;Zhouping Wang ,&nbsp;Junsong Yang","doi":"10.1016/j.crbiot.2025.100308","DOIUrl":"10.1016/j.crbiot.2025.100308","url":null,"abstract":"<div><div>Edible blood products from domestic livestock are high-value derivatives produced during slaughter, with significant potential in biomedicine and disease treatment. Livestock blood is rich in proteins, peptides, iron, vitamins and other bioactive substances. Among them, functional proteins and peptides have been widely studied for their prominent roles in drug delivery, immune regulation, anti-infection and antioxidation. They significantly impact cancer treatment and immune regulation, providing valuable resources for innovation in the food and pharmaceutical industries. The development and utilization of livestock blood products offer innovative solutions for disease treatment and promote the sustainable development of the food and pharmaceutical industries by reducing the environmental burden of blood waste. However, challenges such as the complexity of blood composition, the variability of bioactive compound effectiveness, and the need for advanced purification techniques limit their broader applications. Additionally, regulatory and safety concerns must be addressed for therapeutic suitability. This review highlights the recent advancements in the biomedical applications of livestock blood products in biomedicine, addressing these challenges and emphasizing their potential to reduce environmental waste and promote sustainable development. This review consolidates current knowledge to provide a foundation for future research, fostering innovation in disease intervention and therapy while addressing utilization barriers.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100308"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the cis and trans isomers of two hydantoin compounds with anticonvulsant potency","authors":"Jana Tchekalarova ,&nbsp;Tsveta Stoyanova ,&nbsp;Petar Todorov","doi":"10.1016/j.crbiot.2025.100317","DOIUrl":"10.1016/j.crbiot.2025.100317","url":null,"abstract":"<div><div>Hydantoins are a class of compounds renowned for their diverse medical applications, particularly in neurology. Phenytoin (Dilantin) is one of the most well-known hydantoins, primarily used to stabilize neuronal membranes and prevent the spread of seizure activity. Recently, our team synthesized and studied the physicochemical properties of two compounds, 3-amino-5,5′-dimethylhydantoin Schiff Bases (<strong>SB1</strong> and <strong>SB2</strong>). We hypothesized that <strong>SB1</strong> and <strong>SB2</strong> possess anticonvulsant properties, with the cis forms expected to be more active than their trans forms. We conducted experiments using the maximal electroshock (MES) test and corneal kindling in mice to test this hypothesis. The results showed that the cis isomers of <strong>SB1</strong> and <strong>SB2</strong> exhibited more potent activity against seizure spread than their trans isomers, and this was observed at doses that did not induce a sedative effect. Furthermore, both trans and cis isomers of <strong>SB1</strong> but not <strong>SB2</strong> compound demonstrated the ability to suppress seizures in the corneal kindling test. Our findings suggest that the trans-cis conversion of the hydantoin compounds <strong>SB1</strong> and <strong>SB2</strong> is crucial for their effectiveness in preventing seizure spread in the MES test and that of <strong>SB1</strong> in the corneal kindling mouse model.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100317"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}